AlfalfaGEDB | Msa0295840

M. sativa cultivar zhongmu-4 / Msa0295840

KEGG: K13348
GO: GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005778 GO:0005779 GO:0016020 GO:0016021 GO:0031090 GO:0031224 GO:0031231 GO:0031300 GO:0031301 GO:0031903 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0098588 GO:0098805
PFAM: PF04117.15

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0295840	XP_013464759.1	94.851	369	13	3	1	366	1	366	0.0	680

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0295840	sp\|Q6CIY7\|SYM1_KLULA	28.655	171	116	3	176	342	6	174	9.19e-16	79.0

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
No data available in table

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0000220	Msa0295840	0.849550	3.009161e-60	-8.615850e-47
Msa0005160	Msa0295840	0.839644	1.401129e-57	-8.615850e-47
Msa0006820	Msa0295840	0.815447	9.202022e-52	-8.615850e-47
Msa0008850	Msa0295840	0.845973	2.906619e-59	-8.615850e-47
Msa0012740	Msa0295840	0.802452	5.572244e-49	-8.615850e-47
Msa0016830	Msa0295840	0.804365	2.236726e-49	-8.615850e-47
Msa0017120	Msa0295840	0.818781	1.638568e-52	-8.615850e-47
Msa0018870	Msa0295840	0.802628	5.126278e-49	-8.615850e-47
Msa0021710	Msa0295840	0.822221	2.659650e-53	-8.615850e-47
Msa0030000	Msa0295840	0.813615	2.340013e-51	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0295840	MtrunA17_Chr2g0318131	94.851	369	13	3	1	366	1	366	0.0	680
Msa0295840	MtrunA17_Chr2g0324001	73.663	243	64	0	123	365	69	311	2.44e-137	396
Msa0295840	MtrunA17_Chr6g0465041	23.810	168	128	0	180	347	56	223	3.38e-15	74.7
Msa0295840	MtrunA17_Chr1g0187331	29.032	186	120	6	177	359	9	185	1.38e-14	72.0
Msa0295840	MtrunA17_Chr1g0207521	27.273	176	125	2	180	353	85	259	2.53e-14	72.8

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0295840	AT1G52870.2	64.463	363	110	6	2	359	4	352	1.80e-163	465
Msa0295840	AT4G03410.2	74.597	248	62	1	121	368	72	318	3.88e-142	410
Msa0295840	AT4G03410.1	75.949	237	57	0	121	357	72	308	2.33e-140	404
Msa0295840	AT1G52870.1	59.688	320	106	7	2	316	4	305	7.18e-128	372
Msa0295840	AT5G43140.1	24.713	174	130	1	180	353	82	254	8.43e-14	71.6
Msa0295840	AT4G33905.1	26.111	180	128	2	180	359	86	260	1.12e-13	71.2
Msa0295840	AT2G14860.1	26.901	171	124	1	180	350	77	246	3.39e-13	69.7

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Find 108 sgRNAs with CRISPR-Local

Find 208 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACTGCATGGTCGGCAGTT+TGG	0.384594	2_3:+48620603	Msa0295840:CDS
AAATTGAAGGGAGGAAAGTG+TGG	0.672436	2_3:-48618822	None:intergenic
AACCCCACTAATCACCATCT+TGG	0.505082	2_3:-48620180	None:intergenic
AACTCACCAACTCACTTCAA+TGG	0.515712	2_3:+48618774	Msa0295840:exon
AATATTACAGACCAGCAAGA+AGG	0.568875	2_3:+48618988	Msa0295840:CDS
AATGATGAGAATTTCTCAAT+TGG	0.415255	2_3:+48622164	Msa0295840:three_prime_UTR
AATGGATTTGGAGCGAATGA+AGG	0.492577	2_3:+48619078	Msa0295840:CDS
AATTCATTGAAAATATTGAT+TGG	0.360409	2_3:-48620671	None:intergenic
ACACAACTGGTTAGCTTATG+AGG	0.392465	2_3:+48620130	Msa0295840:CDS
ACGACGCAAGTGAATGGATT+TGG	0.380452	2_3:+48619066	Msa0295840:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TAATGAGAATGAAGAGAATG+TGG	+	chr2_3:48619146-48619165	Msa0295840:CDS	30.0%
GATTAAATTTGGTTGTACTG+TGG	+	chr2_3:48619344-48619363	Msa0295840:intron	30.0%
AAAATAAGTCAATCCAAACC+CGG	-	chr2_3:48619407-48619426	None:intergenic	30.0%
AAAAACTAAGCCAATCCAAA+CGG	+	chr2_3:48619642-48619661	Msa0295840:intron	30.0%
TAGACCAACTTTAAATTAGC+AGG	-	chr2_3:48619941-48619960	None:intergenic	30.0%
AGCTAATCTAAATTAGCAAC+TGG	-	chr2_3:48620004-48620023	None:intergenic	30.0%
GGAAAAGAACATTGTTCATA+TGG	-	chr2_3:48620458-48620477	None:intergenic	30.0%
ATTCAAGAACAATGCAAGAA+AGG	-	chr2_3:48620479-48620498	None:intergenic	30.0%
TTCTTGAATTGTCTGACTTA+TGG	+	chr2_3:48620490-48620509	Msa0295840:intron	30.0%
AGCATTTACTATACAGTTGT+GGG	+	chr2_3:48620629-48620648	Msa0295840:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_3	gene	48618650	48622217	48618650	ID=Msa0295840;Name=Msa0295840
chr2_3	mRNA	48618650	48622217	48618650	ID=Msa0295840-mRNA-1;Parent=Msa0295840;Name=Msa0295840-mRNA-1;_AED=0.05;_eAED=0.05;_QI=143\|0.83\|0.85\|1\|0.83\|0.85\|7\|210\|442
chr2_3	exon	48618650	48619275	48618650	ID=Msa0295840-mRNA-1:exon:15257;Parent=Msa0295840-mRNA-1
chr2_3	exon	48620092	48620232	48620092	ID=Msa0295840-mRNA-1:exon:15258;Parent=Msa0295840-mRNA-1
chr2_3	exon	48620336	48620449	48620336	ID=Msa0295840-mRNA-1:exon:15259;Parent=Msa0295840-mRNA-1
chr2_3	exon	48620548	48620721	48620548	ID=Msa0295840-mRNA-1:exon:15260;Parent=Msa0295840-mRNA-1
chr2_3	exon	48620853	48620968	48620853	ID=Msa0295840-mRNA-1:exon:15261;Parent=Msa0295840-mRNA-1
chr2_3	exon	48621606	48621689	48621606	ID=Msa0295840-mRNA-1:exon:15262;Parent=Msa0295840-mRNA-1
chr2_3	exon	48621791	48622217	48621791	ID=Msa0295840-mRNA-1:exon:15263;Parent=Msa0295840-mRNA-1
chr2_3	five_prime_UTR	48618650	48618792	48618650	ID=Msa0295840-mRNA-1:five_prime_utr;Parent=Msa0295840-mRNA-1
chr2_3	CDS	48618793	48619275	48618793	ID=Msa0295840-mRNA-1:cds;Parent=Msa0295840-mRNA-1
chr2_3	CDS	48620092	48620232	48620092	ID=Msa0295840-mRNA-1:cds;Parent=Msa0295840-mRNA-1
chr2_3	CDS	48620336	48620449	48620336	ID=Msa0295840-mRNA-1:cds;Parent=Msa0295840-mRNA-1
chr2_3	CDS	48620548	48620721	48620548	ID=Msa0295840-mRNA-1:cds;Parent=Msa0295840-mRNA-1
chr2_3	CDS	48620853	48620968	48620853	ID=Msa0295840-mRNA-1:cds;Parent=Msa0295840-mRNA-1
chr2_3	CDS	48621606	48621689	48621606	ID=Msa0295840-mRNA-1:cds;Parent=Msa0295840-mRNA-1
chr2_3	CDS	48621791	48622007	48621791	ID=Msa0295840-mRNA-1:cds;Parent=Msa0295840-mRNA-1
chr2_3	three_prime_UTR	48622008	48622217	48622008	ID=Msa0295840-mRNA-1:three_prime_utr;Parent=Msa0295840-mRNA-1

Gene Sequence

>Msa0295840

ATGGCTTCCGTTCACAGCACCGTACCTCACCACACTTTCCTCCCTTCAATTTCCAAACCAAAATCAAAATCCATTTCCAAGCCACAAAACTTTCCAGTATCAAGATTCTCCACTAATCCGAATTTCACAAAACGTGCAGCGGTAGTGACGAAAGTATCAACGGAGGAGTTCGACGTAATAGCAGTACAGAGCAACAATATTACAGACCAGCAAGAAGGTTTGATAGTGAGCCGTGTGGAGATGGAAGGTGAAGATGGTGAATTAGCGGCGACGACGACGCAAGTGAATGGATTTGGAGCGAATGAAGGTTTGTTATCGTTGGAAGGTTTTTCGAGTGCTTCGGGTTTGGTTGGTAATGAGAATGAAGAGAATGTGGAGAGTGTGGAGAAGCTTCTAGATAGAAGTATTAATGCTAGTATTGTTCTTGTTGCTGGTACTTTTGCTCTTACGAAGCTTCTTACTATTGATAGTGATTATTGGCATGGATGGACAATCTATGAGATAGTAAGATATGCACCTCTACACAACTGGTTAGCTTATGAGGAAGCTCTTAAGACAAATCCGGTTTTTGCCAAGATGGTGATTAGTGGGGTTGTTTATTCCGTAGGAGACTGGATTGCACAGTGCTTCGAAGGAAAGCCTCTTTTCGAGTTTGATCGTGCACGGATGTTCAGATCAGGGCTTGTTGGATTTGCTCTACATGGCTCTCTTTCTCATTACTATTACCAGTTTTGTGAGGAGCTATTTCCTTACAAAGGATGGTGGGTAGTTCCTGTCAAAGTTGCCTTCGATCAAACTGCATGGTCGGCAGTTTGGAACAGCATTTACTATACAGTTGTGGGGATCCTGCGTTTTGATTCTCCAATCAATATTTTCAATGAATTGAAGGCAACATTTTTCCCCATGTTGACTGCAGGATGGAAGTTGTGGCCATTTGCTCATCTAATAACTTACGGTGTAATACCAGTTGAGCAAAGACTTCTTTGGGTAGATATGATAGAGCTTATTTGGGTGACCATACTTTCAACATATTCAAATGAAAAATCAGAAGCAAGAAGCTCTTCAGAGTCTATAGAAGCGAAATCCACTACATCTGAGGTATGTGGCTTCTGGAGTGAAAAAGAAGAGATATGTGATAGAAAATATTATTATAATGGAATGAAGTTCACGGTGCGCTGGACCTTAATTTGGTTTGGACATAGATTTGTAATGCAGAATATTGAAGAAGCTGATTGTATATGTGTATATGTTGTATCCCTAAGAAATTCATGTATGTTAGGCAACATTATCAACTCTCCTGTAGCCATTACACAATGTAATTGTTCATAA

Protein sequence

>Msa0295840

MASVHSTVPHHTFLPSISKPKSKSISKPQNFPVSRFSTNPNFTKRAAVVTKVSTEEFDVIAVQSNNITDQQEGLIVSRVEMEGEDGELAATTTQVNGFGANEGLLSLEGFSSASGLVGNENEENVESVEKLLDRSINASIVLVAGTFALTKLLTIDSDYWHGWTIYEIVRYAPLHNWLAYEEALKTNPVFAKMVISGVVYSVGDWIAQCFEGKPLFEFDRARMFRSGLVGFALHGSLSHYYYQFCEELFPYKGWWVVPVKVAFDQTAWSAVWNSIYYTVVGILRFDSPINIFNELKATFFPMLTAGWKLWPFAHLITYGVIPVEQRLLWVDMIELIWVTILSTYSNEKSEARSSSESIEAKSTTSEVCGFWSEKEEICDRKYYYNGMKFTVRWTLIWFGHRFVMQNIEEADCICVYVVSLRNSCMLGNIINSPVAITQCNCS*