AlfalfaGEDB | Msa0310490

M. sativa cultivar zhongmu-4 / Msa0310490

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0310490	XP_003593210.1	92.347	196	11	1	1	196	183	374	5.02e-126	370

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0012260	Msa0310490	0.806420	8.297118e-50	-8.615850e-47
Msa0030030	Msa0310490	0.803916	2.773660e-49	-8.615850e-47
Msa0039200	Msa0310490	0.802380	5.767422e-49	-8.615850e-47
Msa0060970	Msa0310490	0.812027	5.211102e-51	-8.615850e-47
Msa0077330	Msa0310490	0.807226	5.605020e-50	-8.615850e-47
Msa0086820	Msa0310490	0.801576	8.435053e-49	-8.615850e-47
Msa0133670	Msa0310490	0.806011	1.012008e-49	-8.615850e-47
Msa0133880	Msa0310490	0.816418	5.586931e-52	-8.615850e-47
Msa0139740	Msa0310490	0.805952	1.041081e-49	-8.615850e-47
Msa0149810	Msa0310490	0.816958	4.229135e-52	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0310490	MtrunA17_Chr2g0279241	92.347	196	11	1	1	196	183	374	4.62e-130	370

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0310490	AT5G64230.1	52.717	184	84	2	10	192	197	378	1.12e-58	188
Msa0310490	AT3G19920.1	40.000	165	88	3	28	191	262	416	2.15e-33	123
Msa0310490	AT3G19920.2	40.000	165	88	3	28	191	303	457	4.45e-33	123

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Find 139 sgRNAs with CRISPR-Local

Find 207 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGAAAATAACCAAAATG+GGG	0.656227	2_4:+5318720	Msa0310490:five_prime_UTR
AAAGGGAAGAGATTAGGGTT+CGG	0.546803	2_4:-5318799	None:intergenic
AACAAGGTGATGGAGGAGAG+TGG	0.587077	2_4:+5319256	Msa0310490:five_prime_UTR
AACAGCAAGAACCAACATAG+TGG	0.577713	2_4:-5321345	None:intergenic
AACGGCGGTGGTGGTGAAAA+GGG	0.514502	2_4:-5318816	None:intergenic
AAGAACAGGTTCTTTCTCTT+TGG	0.297051	2_4:+5320990	Msa0310490:CDS
AAGAAGGAATGAAACGGAGG+AGG	0.508172	2_4:-5318925	None:intergenic
AAGGTGAAGGGAGGTGAGGG+TGG	0.541781	2_4:+5319321	Msa0310490:CDS
AAGTGAGGATGATGAAACGG+AGG	0.593185	2_4:-5318892	None:intergenic
AAGTTCTAAGTTATATCAAC+AGG	0.453490	2_4:+5321195	Msa0310490:three_prime_UTR

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CCAAGTAACTTTGATAAAGA+TGG	-	chr2_4:5319672-5319691	None:intergenic	30.0%
CCATCTTTATCAAAGTTACT+TGG	+	chr2_4:5319669-5319688	Msa0310490:intron	30.0%
TTTCAAACCACCTATATTCT+CGG	-	chr2_4:5319695-5319714	None:intergenic	30.0%
AAAATATTAGCTCGAGAATG+TGG	+	chr2_4:5319712-5319731	Msa0310490:intron	30.0%
TTTAAGCCTTTGTACTCATT+AGG	+	chr2_4:5320050-5320069	Msa0310490:intron	30.0%
AACTTTGGATATAATTTCCC+GGG	-	chr2_4:5320096-5320115	None:intergenic	30.0%
TCTTGTCTCCAAGTTTATAA+TGG	+	chr2_4:5320299-5320318	Msa0310490:intron	30.0%
TGGCCAATAAACTTATTATG+GGG	+	chr2_4:5320319-5320338	Msa0310490:intron	30.0%
AAGCCCCATAATAAGTTTAT+TGG	-	chr2_4:5320325-5320344	None:intergenic	30.0%
TAAATAACTTCCAAGGATTG+TGG	+	chr2_4:5320457-5320476	Msa0310490:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_4	gene	5318632	5321387	5318632	ID=Msa0310490;Name=Msa0310490
chr2_4	mRNA	5318632	5321387	5318632	ID=Msa0310490-mRNA-1;Parent=Msa0310490;Name=Msa0310490-mRNA-1;_AED=0.01;_eAED=0.01;_QI=653\|1\|1\|1\|1\|1\|2\|264\|196
chr2_4	exon	5318632	5319522	5318632	ID=Msa0310490-mRNA-1:exon:1875;Parent=Msa0310490-mRNA-1
chr2_4	exon	5320771	5321387	5320771	ID=Msa0310490-mRNA-1:exon:1876;Parent=Msa0310490-mRNA-1
chr2_4	five_prime_UTR	5318632	5319284	5318632	ID=Msa0310490-mRNA-1:five_prime_utr;Parent=Msa0310490-mRNA-1
chr2_4	CDS	5319285	5319522	5319285	ID=Msa0310490-mRNA-1:cds;Parent=Msa0310490-mRNA-1
chr2_4	CDS	5320771	5321123	5320771	ID=Msa0310490-mRNA-1:cds;Parent=Msa0310490-mRNA-1
chr2_4	three_prime_UTR	5321124	5321387	5321124	ID=Msa0310490-mRNA-1:three_prime_utr;Parent=Msa0310490-mRNA-1

Gene Sequence

>Msa0310490

ATGGTGAGGAGGAGGGTGTGGAGGGTGGTGGGGTGGAAGGTGAAGGGAGGTGAGGGTGGTTTAAGTGGTGGTGTTCCGGCGGAGAAATTGGCAGCGGAGTTGGTTTGGATGGTGAAGAAGATGGTGGAGTGTGGTTGTGCTGATGAAGCTCTTCAGAGATGGGCGGTGGCGGATAATTTAGGGTTTCTTGCTCTTACTGCTGATCCTCGTTTGCAGGCTTCGCTTGTTAAACTTGCAGCATTTCTGTTCAAAGAAGCCAAAGATTTTGGCGCAGACGATATTGAGGAAAGCAAGATGAAGCAATGCATGCAGGTAAAGTTAAAGATGTTACAGACATGGCTGCCATTGTTGTGCAGGGCCAGTAATGGCATCGATGCCCCAGCATTGAGCATCAATGAGAGGGCTGAGTTGGAGAGGGTGTTGGAAAGCATTATAGAGGGGTTGGAACAAGCGAAGCAAGAACAGGTTCTTTCTCTTTGGCTCCACCATTTTACACATTGCTCATCCTCTGACTGGCCAAACCTCCAATCATGTTATGCTCGCTGGTGCTGTAAATCTCGCAAGCAGTTGCTGCTTTCGAGTGAAAATTGA

Protein sequence

>Msa0310490

MVRRRVWRVVGWKVKGGEGGLSGGVPAEKLAAELVWMVKKMVECGCADEALQRWAVADNLGFLALTADPRLQASLVKLAAFLFKEAKDFGADDIEESKMKQCMQVKLKMLQTWLPLLCRASNGIDAPALSINERAELERVLESIIEGLEQAKQEQVLSLWLHHFTHCSSSDWPNLQSCYARWCCKSRKQLLLSSEN*