AlfalfaGEDB | Msa0789100

M. sativa cultivar zhongmu-4 / Msa0789100

KEGG: K21843
GO: GO:0003674 GO:0005488 GO:0005515 GO:0005516
PFAM: PF14559.9

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0789100	sp\|Q8GZN1\|NPG1_ARATH	68.990	703	199	4	12	711	18	704	0.0	951

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0789100	G7K6T7	94.515	711	24	1	1	711	1	696	0.0	1373

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0000050	Msa0789100	0.806992	6.282062e-50	-8.615850e-47
Msa0000880	Msa0789100	0.816583	5.131059e-52	-8.615850e-47
Msa0001960	Msa0789100	0.803836	2.882769e-49	-8.615850e-47
Msa0002710	Msa0789100	0.800029	1.745704e-48	-8.615850e-47
Msa0024710	Msa0789100	0.813681	2.263698e-51	-8.615850e-47
Msa0025720	Msa0789100	0.814209	1.730987e-51	-8.615850e-47
Msa0027820	Msa0789100	0.826851	2.162180e-54	-8.615850e-47
Msa0035120	Msa0789100	0.841708	4.033889e-58	-8.615850e-47
Msa0035130	Msa0789100	0.829984	3.790392e-55	-8.615850e-47
Msa0051020	Msa0789100	0.818583	1.817300e-52	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0789100	MtrunA17_Chr5g0409291	96.839	601	19	0	1	601	1	601	0.0	1199
Msa0789100	MtrunA17_Chr8g0369551	75.497	604	142	1	1	604	2	599	0.0	934
Msa0789100	MtrunA17_Chr1g0183461	47.605	689	338	6	26	707	40	712	0.0	597
Msa0789100	MtrunA17_Chr1g0187271	44.476	715	358	12	8	707	41	731	0.0	568
Msa0789100	MtrunA17_Chr7g0261581	46.043	695	355	5	17	707	49	727	0.0	560
Msa0789100	MtrunA17_Chr1g0152501	40.000	720	401	8	2	707	14	716	5.13e-160	479
Msa0789100	MtrunA17_Chr5g0409281	95.327	107	5	0	605	711	8	114	4.89e-67	216
Msa0789100	MtrunA17_Chr8g0369541	72.973	111	29	1	601	711	16	125	5.64e-48	165

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0789100	AT2G43040.2	68.990	703	199	4	12	711	18	704	0.0	951
Msa0789100	AT2G43040.1	68.990	703	199	4	12	711	18	704	0.0	951
Msa0789100	AT4G28600.1	44.176	704	361	7	18	707	53	738	3.80e-177	524
Msa0789100	AT4G28600.2	44.176	704	361	7	18	707	53	738	3.80e-177	524
Msa0789100	AT4G28600.3	43.724	709	362	7	18	707	53	743	1.79e-176	522
Msa0789100	AT1G27460.1	39.944	716	384	10	2	707	14	693	1.47e-161	483
Msa0789100	AT1G27460.3	39.944	716	380	10	2	707	14	689	1.17e-160	480
Msa0789100	AT1G27460.2	42.407	540	287	7	2	531	14	539	8.64e-131	398

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Find 166 sgRNAs with CRISPR-Local

Find 424 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACGAGGGTTGCAACCGT+TGG	0.559120	5_2:+76818587	None:intergenic
AAAATGGGATCCTTCAATCA+TGG	0.509467	5_2:-76817689	Msa0789100:CDS
AACAAGATCTAAGGGACACT+TGG	0.560208	5_2:+76820781	None:intergenic
AACCGACCAATCGTATGGCT+TGG	0.538779	5_2:-76815290	Msa0789100:CDS
AACGGAAGTTTGGCTACATA+TGG	0.398438	5_2:+76817645	None:intergenic
AACTCTGGAATCATTGGAGG+AGG	0.552489	5_2:-76817260	Msa0789100:CDS
AAGAATCATTAAAAGCAAAA+TGG	0.376723	5_2:+76817738	None:intergenic
AAGACCCAAAGGATTTCCTC+TGG	0.493206	5_2:+76816903	None:intergenic
AAGATTTCCAGAAGCTGAAG+TGG	0.541223	5_2:-76817065	Msa0789100:CDS
AAGCCCAGAGGAAATCCTTT+GGG	0.452553	5_2:-76816907	Msa0789100:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AACAACAACAACAACAACAT+AGG	-	chr5_2:76814826-76814845	Msa0789100:three_prime_UTR	30.0%
GTGAAATCAAACAAGATCTA+AGG	+	chr5_2:76814883-76814902	None:intergenic	30.0%
AAATTAGCAGATCTTGGAAA+TGG	+	chr5_2:76814997-76815016	None:intergenic	30.0%
AACTCATTATTCACGAAATG+GGG	+	chr5_2:76815097-76815116	None:intergenic	30.0%
AAAAAAAACTCCATTTCCCA+CGG	+	chr5_2:76815165-76815184	None:intergenic	30.0%
TGAGTTTGATAAAATCATGG+TGG	-	chr5_2:76815261-76815280	Msa0789100:CDS	30.0%
ATTCAATTCAAACATGCACT+TGG	-	chr5_2:76815367-76815386	Msa0789100:CDS	30.0%
TGAAACTTATGTGAAAAGTG+TGG	-	chr5_2:76815454-76815473	Msa0789100:CDS	30.0%
TCAATTGATTGCTCCAAAAT+GGG	+	chr5_2:76815808-76815827	None:intergenic	30.0%
TTCAATTGATTGCTCCAAAA+TGG	+	chr5_2:76815809-76815828	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_2	gene	76814717	76820957	76814717	ID=Msa0789100;Name=Msa0789100
chr5_2	mRNA	76814717	76820957	76814717	ID=Msa0789100-mRNA-1;Parent=Msa0789100;Name=Msa0789100-mRNA-1;_AED=0.15;_eAED=0.15;_QI=248\|0.8\|0.83\|1\|0.8\|0.66\|6\|453\|711
chr5_2	exon	76814717	76815492	76814717	ID=Msa0789100-mRNA-1:exon:23386;Parent=Msa0789100-mRNA-1
chr5_2	exon	76815741	76815933	76815741	ID=Msa0789100-mRNA-1:exon:23385;Parent=Msa0789100-mRNA-1
chr5_2	exon	76816886	76818096	76816886	ID=Msa0789100-mRNA-1:exon:23384;Parent=Msa0789100-mRNA-1
chr5_2	exon	76818452	76818704	76818452	ID=Msa0789100-mRNA-1:exon:23383;Parent=Msa0789100-mRNA-1
chr5_2	exon	76820014	76820187	76820014	ID=Msa0789100-mRNA-1:exon:23382;Parent=Msa0789100-mRNA-1
chr5_2	exon	76820728	76820957	76820728	ID=Msa0789100-mRNA-1:exon:23381;Parent=Msa0789100-mRNA-1
chr5_2	five_prime_UTR	76820728	76820957	76820728	ID=Msa0789100-mRNA-1:five_prime_utr;Parent=Msa0789100-mRNA-1
chr5_2	five_prime_UTR	76820170	76820187	76820170	ID=Msa0789100-mRNA-1:five_prime_utr;Parent=Msa0789100-mRNA-1
chr5_2	CDS	76820014	76820169	76820014	ID=Msa0789100-mRNA-1:cds;Parent=Msa0789100-mRNA-1
chr5_2	CDS	76818452	76818704	76818452	ID=Msa0789100-mRNA-1:cds;Parent=Msa0789100-mRNA-1
chr5_2	CDS	76816886	76818096	76816886	ID=Msa0789100-mRNA-1:cds;Parent=Msa0789100-mRNA-1
chr5_2	CDS	76815741	76815933	76815741	ID=Msa0789100-mRNA-1:cds;Parent=Msa0789100-mRNA-1
chr5_2	CDS	76815170	76815492	76815170	ID=Msa0789100-mRNA-1:cds;Parent=Msa0789100-mRNA-1
chr5_2	three_prime_UTR	76814717	76815169	76814717	ID=Msa0789100-mRNA-1:three_prime_utr;Parent=Msa0789100-mRNA-1

Gene Sequence

>Msa0789100

ATGGAGTCCTCTGGTGAGCATGAAAGGTTGTCAGTTAGAGAGGTTCATGCAAATGGAAGCCGCATGGCTGCATCAGAAGTTGAGGCTAAGCTCGATGAAGGAAATATTCAAGAAGCAGAATCTGCATTGCGAGAAGGGTTGTCACTCAATTTTGAGGAAGCAAGGGCTCTTCTTGGGAAGTTGGAGTATCAGAGAGGTAATGTAGAAGGTGCTCTTCGTGTCTTTGATGGGATCGACCTCCATGCTGCCATCCAACGGTTGCAACCCTCGTTTTCTGAAAAATCGCCTGTCAAAAAAGGTCGTACTCGCACAGAATCACCTTCTTCAGTCTCTCAGCATGCTGCTACTTTGGTGCTTGAAGCCATCTACTTAAAAGCCAAGTCTCAACAAAAACTGGGGAAATTTACTGAAGCTGCTAATGACTGTAAACGTATTCTTGATGCTGTTGAGAAGATATTTGATCAGGGCATACCTGATTTTCAAGTGGACGGTAAACTGCAAGAGATAGTCAGCCATGCAGTAGAGCTCCTTCCAGAGCTTTGGAAACAAGGAGGTTGCTATGACGAGGCAATATCTGCTTATAGGCGTGCCCTTCTTAGTCAATGGAACCTCGATAATGATTGCTGTTCAAGAATTCAGAAATCATTTGTTGTTTTTCTGTTGTACAGTGGTGTGGAGGCAAGTCCTCCTAGTTTAGCAGTTCAGGTTGATGGTTCTTATGTGCCTAAAAATAATCTCGAAGAGGCCATTTTGCTTTTAATGATTCTTATAAGAAAGTTTCGTCATGGTAAGATAAAATGGGATCCTTCAATCATGGAGCACCTAACTTTTGCACTTTCCATATGTAGCCAAACTTCCGTTTTAGCAATGCAATTTGAAGACTTGATGCCTGGAGTATACCATCGTATCGATCGTTGGAATTCTTTAGCTTTATGTCATTGTGCTGCCGGACAAAATGCCAGTGCTTTGAATCTGCTAAGGAAGTCCTTGCACAAACACGAACGACCAGATGACCTTACATCGTTATTATTAGCTGCTAAGATCTGCAGTGAGAATACCTATCTTGCCGGTGAGGGAGTCAGCCATGCGCAGAGGGCAATCAAGAATGCTCATGGTCCAAATGAGCATTTAAAGGGTGTTGCTCTTCGCATGTTGGGACTTTGTCTTGGAAAGCAAGCTAAAGTTGCTTCCTCTGACTTCGAGAGGTCTCGGTTTCAGTCCAAAACTCTGGAATCATTGGAGGAGGCAACTAGATTGGAGAAAAATAATTCTGATCTGATTTTTGAGTTGGCGGTTCAGTACGCTGAGCACCGTAATCTGACCGCTGCTTTGCGGTCTGCGCGGCATTTCTTCAACGAAACTGGCGGCTCAATAGTGAAAGCTTGGATATTGCTTGCTCTGATTTTGTCGGCACAACAAAGATTTCCAGAAGCTGAAGTGGTGACAGATGCTGCTTTAGATCAGACTGTGAGATGGGAGCAGGGGCCTCTGCTTAAGCTTAAAGCGAAGCTGAAGATCGCCCAATCAAAACCGATGGATGCTATTGAAACCTATCGCTACCTTCTTGCATTGGTTCAAGCCCAGAGGAAATCCTTTGGGTCTTACAAAATTTGTTCAAAGGTTGAAGATGATAAAGTTAATGAATTTGATATTTGGCATGGTCTTGCAAATTTATATGCCAGCCTTTCTCGTTGGAACGATGCTGAAATCTGTTTGCAAAAGGCCAGAGAGTTGAAGGAGCACTCTGCAGAAATTTTGCGCACTGAAGGCAAGACTAAATCCTTTGTGCTGTTTAAGTTTTTCGTTCTAGCATGTATTTTGTTTGAAGGACGCGGGCAAAATCAAGAAGCTCTCAACGCTACTATTAATGCTATACTACTTGAACCAAACCATGTTCCAAGCAAAATCTTAATGAGTGCTTTGATTCATAAAATGGGCTCAAAGGCTTTGCCTGCTGCTGCGAGAAGCATGCTGTCTGATGCGCTTAGAATCGAACCGACCAATCGTATGGCTTGGTATTACTTGGGATTAATTCACAAGCACGATGGCCGAATGGGTGATGCTGCGGACTGCTTCCAGGCAGCTTCCATGCTGGAAGAATTTGATCCCATCGAAAGTTTCAGCTCCATACTTTGA

Protein sequence

>Msa0789100

MESSGEHERLSVREVHANGSRMAASEVEAKLDEGNIQEAESALREGLSLNFEEARALLGKLEYQRGNVEGALRVFDGIDLHAAIQRLQPSFSEKSPVKKGRTRTESPSSVSQHAATLVLEAIYLKAKSQQKLGKFTEAANDCKRILDAVEKIFDQGIPDFQVDGKLQEIVSHAVELLPELWKQGGCYDEAISAYRRALLSQWNLDNDCCSRIQKSFVVFLLYSGVEASPPSLAVQVDGSYVPKNNLEEAILLLMILIRKFRHGKIKWDPSIMEHLTFALSICSQTSVLAMQFEDLMPGVYHRIDRWNSLALCHCAAGQNASALNLLRKSLHKHERPDDLTSLLLAAKICSENTYLAGEGVSHAQRAIKNAHGPNEHLKGVALRMLGLCLGKQAKVASSDFERSRFQSKTLESLEEATRLEKNNSDLIFELAVQYAEHRNLTAALRSARHFFNETGGSIVKAWILLALILSAQQRFPEAEVVTDAALDQTVRWEQGPLLKLKAKLKIAQSKPMDAIETYRYLLALVQAQRKSFGSYKICSKVEDDKVNEFDIWHGLANLYASLSRWNDAEICLQKARELKEHSAEILRTEGKTKSFVLFKFFVLACILFEGRGQNQEALNATINAILLEPNHVPSKILMSALIHKMGSKALPAAARSMLSDALRIEPTNRMAWYYLGLIHKHDGRMGDAADCFQAASMLEEFDPIESFSSIL*