AlfalfaGEDB | Msa0271300

M. sativa cultivar zhongmu-4 / Msa0271300

KEGG: K03841
GO: -
PFAM: PF00316.23

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0271300	XP_003593121.1	98.538	342	5	0	1	342	1	342	0.0	696

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0271300	sp\|Q42649\|F16P2_BETVU	82.353	340	60	0	1	340	1	340	0.0	596

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0271300	G7IL84	98.538	342	5	0	1	342	1	342	0.0	696

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TFs/TRs:

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Gene ID	Type	Classification
Msa0271300	TF	C3H

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0029010	Msa0271300	0.818056	2.392203e-52	-8.615850e-47
Msa0059880	Msa0271300	0.805174	1.516119e-49	-8.615850e-47
Msa0064990	Msa0271300	0.801791	7.621357e-49	-8.615850e-47
Msa0076640	Msa0271300	0.837817	4.156253e-57	-8.615850e-47
Msa0076650	Msa0271300	0.803089	4.117307e-49	-8.615850e-47
Msa0087900	Msa0271300	0.803213	3.881041e-49	-8.615850e-47
Msa0146210	Msa0271300	0.835429	1.686901e-56	-8.615850e-47
Msa0150910	Msa0271300	0.814054	1.872915e-51	-8.615850e-47
Msa0169050	Msa0271300	0.800748	1.245830e-48	-8.615850e-47
Msa0224210	Msa0271300	0.814906	1.213780e-51	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0271300	MtrunA17_Chr2g0278581	98.538	342	5	0	1	342	1	342	0.0	696
Msa0271300	MtrunA17_Chr6g0463901	84.776	335	51	0	1	335	1	335	0.0	601
Msa0271300	MtrunA17_Chr7g0241071	50.585	342	151	2	11	335	71	411	1.19e-116	343
Msa0271300	MtrunA17_Chr4g0049821	42.258	310	156	6	32	331	87	383	6.13e-76	239
Msa0271300	MtrunA17_Chr3g0113101	30.961	281	173	8	67	342	116	380	4.07e-23	99.0

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0271300	AT1G43670.1	80.826	339	65	0	1	339	1	339	0.0	584
Msa0271300	AT3G54050.2	50.588	340	152	3	11	335	79	417	1.87e-116	343
Msa0271300	AT3G54050.1	50.588	340	152	3	11	335	79	417	1.87e-116	343
Msa0271300	AT5G64380.1	40.881	318	168	8	32	339	89	396	7.16e-74	234
Msa0271300	AT3G55800.1	31.449	283	161	10	67	338	123	383	4.59e-23	99.4

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Find 69 sgRNAs with CRISPR-Local

Find 179 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGATTTATTCTGTGAATG+AGG	0.439092	2_3:+2605214	Msa0271300:CDS
AAAGCCCAAATGGAAAGCTA+AGG	0.593648	2_3:+2605599	Msa0271300:CDS
AACAAAACTCACTTGAACAT+TGG	0.470224	2_3:-2603318	None:intergenic
AACAATGTGATTGATGAGAA+TGG	0.568531	2_3:-2603111	None:intergenic
AACTCCACAATCAATGTTTG+AGG	0.474075	2_3:-2604586	None:intergenic
AAGATTTATTCTGTGAATGA+GGG	0.501287	2_3:+2605215	Msa0271300:CDS
AAGCTTTCACAGGAAAACAA+AGG	0.624169	2_3:+2606018	Msa0271300:CDS
AATATGAACTCTCCAAGGGA+AGG	0.611205	2_3:-2605046	None:intergenic
AATGCATGTACCTTGATGTT+TGG	0.391145	2_3:-2605076	None:intergenic
ACTGACTTGCCACGCTGTGC+TGG	0.263816	2_3:-2604446	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AACAATGTGATTGATGAGAA+TGG	-	chr2_3:2603114-2603133	None:intergenic	30.0%
TGTTTGTTCTTCTGTTAACA+AGG	+	chr2_3:2603148-2603167	Msa0271300:CDS	30.0%
AACAAAACTCACTTGAACAT+TGG	-	chr2_3:2603321-2603340	None:intergenic	30.0%
AAATACTTCCAAAACATGTG+CGG	+	chr2_3:2603756-2603775	Msa0271300:intron	30.0%
AACTTGATGTAACAGAGATA+AGG	+	chr2_3:2603826-2603845	Msa0271300:intron	30.0%
ACATTTCACAAAGTTTCAAG+AGG	+	chr2_3:2604116-2604135	Msa0271300:intron	30.0%
TCTATTCACCACTATTTAAG+GGG	+	chr2_3:2604172-2604191	Msa0271300:intron	30.0%
ACAAAATAAAGCAAACTCGA+GGG	-	chr2_3:2604281-2604300	None:intergenic	30.0%
AACAAAATAAAGCAAACTCG+AGG	-	chr2_3:2604282-2604301	None:intergenic	30.0%
ATTGATTGTGGAGTTTCAAT+AGG	+	chr2_3:2604594-2604613	Msa0271300:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_3	gene	2603011	2606419	2603011	ID=Msa0271300;Name=Msa0271300
chr2_3	mRNA	2603011	2606419	2603011	ID=Msa0271300-mRNA-1;Parent=Msa0271300;Name=Msa0271300-mRNA-1;_AED=0.12;_eAED=0.12;_QI=0\|0.18\|0.08\|1\|1\|1\|12\|0\|342
chr2_3	exon	2603011	2603169	2603011	ID=Msa0271300-mRNA-1:exon:1310;Parent=Msa0271300-mRNA-1
chr2_3	exon	2603281	2603328	2603281	ID=Msa0271300-mRNA-1:exon:1311;Parent=Msa0271300-mRNA-1
chr2_3	exon	2604192	2604266	2604192	ID=Msa0271300-mRNA-1:exon:1312;Parent=Msa0271300-mRNA-1
chr2_3	exon	2604401	2604462	2604401	ID=Msa0271300-mRNA-1:exon:1313;Parent=Msa0271300-mRNA-1
chr2_3	exon	2604554	2604620	2604554	ID=Msa0271300-mRNA-1:exon:1314;Parent=Msa0271300-mRNA-1
chr2_3	exon	2604725	2604838	2604725	ID=Msa0271300-mRNA-1:exon:1315;Parent=Msa0271300-mRNA-1
chr2_3	exon	2605001	2605087	2605001	ID=Msa0271300-mRNA-1:exon:1316;Parent=Msa0271300-mRNA-1
chr2_3	exon	2605200	2605270	2605200	ID=Msa0271300-mRNA-1:exon:1317;Parent=Msa0271300-mRNA-1
chr2_3	exon	2605380	2605445	2605380	ID=Msa0271300-mRNA-1:exon:1318;Parent=Msa0271300-mRNA-1
chr2_3	exon	2605534	2605620	2605534	ID=Msa0271300-mRNA-1:exon:1319;Parent=Msa0271300-mRNA-1
chr2_3	exon	2605965	2606040	2605965	ID=Msa0271300-mRNA-1:exon:1320;Parent=Msa0271300-mRNA-1
chr2_3	exon	2606303	2606419	2606303	ID=Msa0271300-mRNA-1:exon:1321;Parent=Msa0271300-mRNA-1
chr2_3	CDS	2603011	2603169	2603011	ID=Msa0271300-mRNA-1:cds;Parent=Msa0271300-mRNA-1
chr2_3	CDS	2603281	2603328	2603281	ID=Msa0271300-mRNA-1:cds;Parent=Msa0271300-mRNA-1
chr2_3	CDS	2604192	2604266	2604192	ID=Msa0271300-mRNA-1:cds;Parent=Msa0271300-mRNA-1
chr2_3	CDS	2604401	2604462	2604401	ID=Msa0271300-mRNA-1:cds;Parent=Msa0271300-mRNA-1
chr2_3	CDS	2604554	2604620	2604554	ID=Msa0271300-mRNA-1:cds;Parent=Msa0271300-mRNA-1
chr2_3	CDS	2604725	2604838	2604725	ID=Msa0271300-mRNA-1:cds;Parent=Msa0271300-mRNA-1
chr2_3	CDS	2605001	2605087	2605001	ID=Msa0271300-mRNA-1:cds;Parent=Msa0271300-mRNA-1
chr2_3	CDS	2605200	2605270	2605200	ID=Msa0271300-mRNA-1:cds;Parent=Msa0271300-mRNA-1
chr2_3	CDS	2605380	2605445	2605380	ID=Msa0271300-mRNA-1:cds;Parent=Msa0271300-mRNA-1
chr2_3	CDS	2605534	2605620	2605534	ID=Msa0271300-mRNA-1:cds;Parent=Msa0271300-mRNA-1
chr2_3	CDS	2605965	2606040	2605965	ID=Msa0271300-mRNA-1:cds;Parent=Msa0271300-mRNA-1
chr2_3	CDS	2606303	2606419	2606303	ID=Msa0271300-mRNA-1:cds;Parent=Msa0271300-mRNA-1

Gene Sequence

>Msa0271300

ATGGATCACCAAGCAGATGCTAACAGAACTGATTTGATGACAATAACTAGGTTTGTTCTGAATGAGCAATCAAAGTATGCTGAATCACGTGGAGATTTCACCATTCTCATCAATCACATTGTTCTTGGCTGCAAATTTGTTTGTTCTTCTGTTAACAAGGCTGGATTGGCAAAAATCCTAGGACTTGCTGGAGACACCAATGTTCAAGGGGAGGAGCAAAAGAAATTGGATGTGATTTCTAATGAAGTATTTGTGAAGGCCTTAATCAGCAGCGGACGCACATGCCTTCTTGTTTCAGAAGAGGTTGACGATGCTATCATTGTGCCCCCAGCACAGCGTGGCAAATACATAGTTGTGTTTGATCCTTTGGATGGTTCCTCAAACATTGATTGTGGAGTTTCAATAGGAACTATCTTTGGTATTTACACAGTGAAAGATACAACTGAAGTTAGCGTTGAAGATGCATTGCAACCTGGTAGCAGTTTGGTAGCAGCTGGTTACTGCATGTATGGAAGCTCTTGCACGTTTGTGCTTAGCACAGGAAATGGAGTGAATGGTTTTACACTTGATCCTTCCCTTGGAGAGTTCATATTGACTCACCCAAACATCAAGATACCAAAGAAAGGAAAGATTTATTCTGTGAATGAGGGAAACGCCAAAAACTGGGATGAACCAACGGCCAAATACGTAGAAAATTGCAAGTTTCCACAAGACAGTTCACCACCAAAGTCTCTTAGGTATATTGGCAGTATGGTAGCCGATGTCCATAGAACATTGCTATATGGTGGTATATTCATGTACCCTGCTGATAAGAAAAGCCCAAATGGAAAGCTAAGGGTTCTGTATGAAGTTTTTCCAATGTCATATTTGATGGAGCAAGCAGGAGGTCAAGCTTTCACAGGAAAACAAAGGGCACTTGACTTGGTTCCTAAGAAAATACATGATCGATCGCCAATATTCCTTGGAAGCTACGATGAAATTGAGCATATAAAAGAGCTTTATGCAGAATCCAAAGAAAATGGTGCATGA

Protein sequence

>Msa0271300

MDHQADANRTDLMTITRFVLNEQSKYAESRGDFTILINHIVLGCKFVCSSVNKAGLAKILGLAGDTNVQGEEQKKLDVISNEVFVKALISSGRTCLLVSEEVDDAIIVPPAQRGKYIVVFDPLDGSSNIDCGVSIGTIFGIYTVKDTTEVSVEDALQPGSSLVAAGYCMYGSSCTFVLSTGNGVNGFTLDPSLGEFILTHPNIKIPKKGKIYSVNEGNAKNWDEPTAKYVENCKFPQDSSPPKSLRYIGSMVADVHRTLLYGGIFMYPADKKSPNGKLRVLYEVFPMSYLMEQAGGQAFTGKQRALDLVPKKIHDRSPIFLGSYDEIEHIKELYAESKENGA*