Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0005690 | Msa0076650 | -0.804764 | 1.846700e-49 | -8.615850e-47 |
| Msa0012780 | Msa0076650 | 0.826650 | 2.415218e-54 | -8.615850e-47 |
| Msa0029010 | Msa0076650 | 0.909764 | 3.969122e-82 | -8.615850e-47 |
| Msa0029020 | Msa0076650 | 0.946473 | 4.317101e-105 | -8.615850e-47 |
| Msa0040550 | Msa0076650 | 0.806554 | 7.776266e-50 | -8.615850e-47 |
| Msa0059880 | Msa0076650 | 0.826069 | 3.321188e-54 | -8.615850e-47 |
| Msa0076640 | Msa0076650 | 0.833059 | 6.628083e-56 | -8.615850e-47 |
| Msa0076650 | Msa0090040 | 0.829509 | 4.948269e-55 | -8.615850e-47 |
| Msa0076650 | Msa0096510 | -0.802981 | 4.334729e-49 | -8.615850e-47 |
| Msa0076650 | Msa0106110 | 0.825986 | 3.475312e-54 | -8.615850e-47 |
| Msa0076650 | Msa0109270 | 0.819165 | 1.340013e-52 | -8.615850e-47 |
| Msa0076650 | Msa0137120 | 0.801705 | 7.938349e-49 | -8.615850e-47 |
| Msa0076650 | Msa0150910 | 0.816997 | 4.144143e-52 | -8.615850e-47 |
| Msa0076650 | Msa0152640 | 0.804428 | 2.170818e-49 | -8.615850e-47 |
| Msa0076650 | Msa0152650 | 0.809353 | 1.972850e-50 | -8.615850e-47 |
| Msa0076650 | Msa0152740 | 0.802758 | 4.819846e-49 | -8.615850e-47 |
| Msa0076650 | Msa0169050 | 0.896881 | 2.408156e-76 | -8.615850e-47 |
| Msa0076650 | Msa0216810 | 0.821673 | 3.562139e-53 | -8.615850e-47 |
| Msa0076650 | Msa0258700 | 0.805477 | 1.310060e-49 | -8.615850e-47 |
| Msa0076650 | Msa0264940 | 0.860029 | 2.745689e-63 | -8.615850e-47 |
| Msa0076650 | Msa0265930 | 0.802866 | 4.577907e-49 | -8.615850e-47 |
| Msa0076650 | Msa0271300 | 0.803089 | 4.117307e-49 | -8.615850e-47 |
| Msa0076650 | Msa0291920 | 0.843397 | 1.437073e-58 | -8.615850e-47 |
| Msa0076650 | Msa0297950 | 0.808626 | 2.824360e-50 | -8.615850e-47 |
| Msa0076650 | Msa0302990 | 0.858044 | 1.079121e-62 | -8.615850e-47 |
| Msa0076650 | Msa0329890 | 0.810310 | 1.228315e-50 | -8.615850e-47 |
| Msa0076650 | Msa0335890 | 0.830279 | 3.210963e-55 | -8.615850e-47 |
| Msa0076650 | Msa0343200 | -0.800001 | 1.768836e-48 | -8.615850e-47 |
| Msa0076650 | Msa0381630 | 0.801178 | 1.017631e-48 | -8.615850e-47 |
| Msa0076650 | Msa0405250 | 0.810605 | 1.060577e-50 | -8.615850e-47 |
| Msa0076650 | Msa0423690 | 0.807502 | 4.897891e-50 | -8.615850e-47 |
| Msa0076650 | Msa0434890 | 0.809085 | 2.252854e-50 | -8.615850e-47 |
| Msa0076650 | Msa0441780 | 0.822758 | 1.995905e-53 | -8.615850e-47 |
| Msa0076650 | Msa0453940 | 0.821249 | 4.463880e-53 | -8.615850e-47 |
| Msa0076650 | Msa0525460 | 0.801466 | 8.887546e-49 | -8.615850e-47 |
| Msa0076650 | Msa0535020 | -0.833183 | 6.172254e-56 | -8.615850e-47 |
| Msa0076650 | Msa0535050 | -0.837462 | 5.125747e-57 | -8.615850e-47 |
| Msa0076650 | Msa0591780 | -0.831763 | 1.388160e-55 | -8.615850e-47 |
| Msa0076650 | Msa0747500 | 0.825220 | 5.277459e-54 | -8.615850e-47 |
| Msa0076650 | Msa0766100 | 0.802303 | 5.980910e-49 | -8.615850e-47 |
| Msa0076650 | Msa0882070 | 0.805373 | 1.377740e-49 | -8.615850e-47 |
| Msa0076650 | Msa0882430 | 0.818423 | 1.975711e-52 | -8.615850e-47 |
| Msa0076650 | Msa0882880 | 0.819917 | 9.030360e-53 | -8.615850e-47 |
| Msa0076650 | Msa0887000 | -0.825330 | 4.971747e-54 | -8.615850e-47 |
| Msa0076650 | Msa0896990 | 0.818897 | 1.542478e-52 | -8.615850e-47 |
| Msa0076650 | Msa0910250 | 0.820685 | 6.019117e-53 | -8.615850e-47 |
| Msa0076650 | Msa0948050 | 0.844819 | 5.967730e-59 | -8.615850e-47 |
| Msa0076650 | Msa0949410 | 0.805931 | 1.051901e-49 | -8.615850e-47 |
| Msa0076650 | Msa0951720 | -0.802771 | 4.788756e-49 | -8.615850e-47 |
| Msa0076650 | Msa0984460 | 0.854574 | 1.124040e-61 | -8.615850e-47 |
| Msa0076650 | Msa0986160 | 0.804427 | 2.171908e-49 | -8.615850e-47 |
| Msa0076650 | Msa0989490 | 0.812525 | 4.058325e-51 | -8.615850e-47 |
| Msa0076650 | Msa0996940 | 0.812931 | 3.306437e-51 | -8.615850e-47 |
| Msa0076650 | Msa1004260 | -0.805785 | 1.128566e-49 | -8.615850e-47 |
| Msa0076650 | Msa1018020 | 0.805652 | 1.203820e-49 | -8.615850e-47 |
| Msa0076650 | Msa1018720 | -0.807739 | 4.363910e-50 | -8.615850e-47 |
| Msa0076650 | Msa1117280 | -0.816636 | 4.991946e-52 | -8.615850e-47 |
| Msa0076650 | Msa1120090 | 0.801755 | 7.751605e-49 | -8.615850e-47 |
| Msa0076650 | Msa1147980 | 0.802827 | 4.663702e-49 | -8.615850e-47 |
| Msa0076650 | Msa1194110 | 0.807018 | 6.202538e-50 | -8.615850e-47 |
| Msa0076650 | Msa1212740 | -0.802115 | 6.538253e-49 | -8.615850e-47 |
| Msa0076650 | Msa1215000 | 0.830412 | 2.980688e-55 | -8.615850e-47 |
| Msa0076650 | Msa1238480 | -0.802245 | 6.147325e-49 | -8.615850e-47 |
| Msa0076650 | Msa1249370 | 0.810852 | 9.378954e-51 | -8.615850e-47 |
| Msa0076650 | Msa1287090 | 0.816282 | 5.993515e-52 | -8.615850e-47 |
| Msa0076650 | Msa1300930 | 0.808013 | 3.814407e-50 | -8.615850e-47 |
| Msa0076650 | Msa1323160 | 0.804222 | 2.396389e-49 | -8.615850e-47 |
| Msa0076650 | Msa1326290 | 0.806275 | 8.901396e-50 | -8.615850e-47 |
| Msa0076650 | Msa1331950 | 0.814831 | 1.261000e-51 | -8.615850e-47 |
| Msa0076650 | Msa1371080 | 0.828846 | 7.163533e-55 | -8.615850e-47 |
| Msa0076650 | Msa1373950 | -0.801683 | 8.021437e-49 | -8.615850e-47 |
| Msa0076650 | Msa1431240 | -0.807465 | 4.989157e-50 | -8.615850e-47 |
| Msa0076650 | Msa1432180 | 0.813772 | 2.161488e-51 | -8.615850e-47 |
| Msa0076650 | Msa1445430 | 0.804991 | 1.656122e-49 | -8.615850e-47 |
| Msa0076650 | Msa1451780 | -0.805942 | 1.046139e-49 | -8.615850e-47 |
| Msa0076650 | Msa1460310 | 0.857950 | 1.151151e-62 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 47 sgRNAs with CRISPR-Local
Find 55 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTGGGACTAGTGGGGTTCTT+TGG | 0.257108 | 1_2:+52826687 | None:intergenic |
| TTATCAATCCAATCCTCTAA+TGG | 0.328408 | 1_2:-52826425 | Msa0076650:CDS |
| GGTTGATTTGAGCTTTGTAT+TGG | 0.360990 | 1_2:+52826708 | None:intergenic |
| ACTGGGAGAACTAGGGCTTT+TGG | 0.362155 | 1_2:+52826755 | None:intergenic |
| TCCTGGGTTGAAGCCATTAG+AGG | 0.369624 | 1_2:+52826412 | None:intergenic |
| ATCACCACAGCCGTCGGAAT+TGG | 0.371692 | 1_2:+52826553 | None:intergenic |
| CGGAATTGGCGAGGGAGCAT+TGG | 0.411697 | 1_2:+52826567 | None:intergenic |
| CAGGTGTTGTTGTTGCAGCC+AGG | 0.418057 | 1_2:+52826512 | None:intergenic |
| GGAATTGGCGAGGGAGCATT+GGG | 0.432376 | 1_2:+52826568 | None:intergenic |
| GCTGGTGGCGGTGTGGGTGA+TGG | 0.441452 | 1_2:+52826469 | None:intergenic |
| TGGGCTGAGCGACACCGTGC+AGG | 0.443405 | 1_2:+52826587 | None:intergenic |
| TGTGGGTGATGGAATTGTCC+GGG | 0.446715 | 1_2:+52826480 | None:intergenic |
| TTGTTGTTGCAGCCAGGCTT+TGG | 0.449409 | 1_2:+52826518 | None:intergenic |
| GGTTGAAGCCATTAGAGGAT+TGG | 0.451183 | 1_2:+52826417 | None:intergenic |
| GTGTGGGTGATGGAATTGTC+CGG | 0.454258 | 1_2:+52826479 | None:intergenic |
| CTCGCCAATTCCGACGGCTG+TGG | 0.458653 | 1_2:-52826557 | Msa0076650:CDS |
| GAAACTGCGACATTTCGTCC+TGG | 0.459544 | 1_2:+52826395 | None:intergenic |
| AGCAAGTTCACCGCTGGTGG+CGG | 0.469786 | 1_2:+52826457 | None:intergenic |
| TTCACCGCTGGTGGCGGTGT+GGG | 0.483515 | 1_2:+52826463 | None:intergenic |
| TGTTTCTCATCACCAAAGCC+TGG | 0.494972 | 1_2:-52826530 | Msa0076650:CDS |
| TCCTCTAATGGCTTCAACCC+AGG | 0.495018 | 1_2:-52826413 | Msa0076650:CDS |
| AAACTGCGACATTTCGTCCT+GGG | 0.498880 | 1_2:+52826396 | None:intergenic |
| AAGTTGGTGCACATGAGAAT+AGG | 0.499306 | 1_2:+52826366 | None:intergenic |
| CTGAACGTCATCGTCGATCT+CGG | 0.517558 | 1_2:-52826665 | Msa0076650:intron |
| ACACCGTGCAGGGCGGTATG+TGG | 0.520199 | 1_2:+52826598 | None:intergenic |
| GTTCTCCCAGTCACAAAAGA+TGG | 0.534725 | 1_2:-52826743 | Msa0076650:CDS |
| TGACGTTCAGTGGGACTAGT+GGG | 0.536843 | 1_2:+52826678 | None:intergenic |
| ATTGTCCGGGGTGACACCAC+AGG | 0.542125 | 1_2:+52826493 | None:intergenic |
| GGGCTGAGCGACACCGTGCA+GGG | 0.548753 | 1_2:+52826588 | None:intergenic |
| ATCTTCAGCAAGTTCACCGC+TGG | 0.549080 | 1_2:+52826451 | None:intergenic |
| TTCAGCAAGTTCACCGCTGG+TGG | 0.561694 | 1_2:+52826454 | None:intergenic |
| GTTCACCGCTGGTGGCGGTG+TGG | 0.562370 | 1_2:+52826462 | None:intergenic |
| AGATCGACGATGACGTTCAG+TGG | 0.573829 | 1_2:+52826668 | None:intergenic |
| TGCTCCCTCGCCAATTCCGA+CGG | 0.580900 | 1_2:-52826563 | Msa0076650:CDS |
| CACAGCCGTCGGAATTGGCG+AGG | 0.587987 | 1_2:+52826558 | None:intergenic |
| CCTCCACATACCGCCCTGCA+CGG | 0.588163 | 1_2:-52826601 | Msa0076650:CDS |
| ACAGCCGTCGGAATTGGCGA+GGG | 0.612804 | 1_2:+52826559 | None:intergenic |
| CAACACCTGTGGTGTCACCC+CGG | 0.614141 | 1_2:-52826498 | Msa0076650:CDS |
| ATGACGTTCAGTGGGACTAG+TGG | 0.614911 | 1_2:+52826677 | None:intergenic |
| CCGTGCAGGGCGGTATGTGG+AGG | 0.617989 | 1_2:+52826601 | None:intergenic |
| ATCACCCACACCGCCACCAG+CGG | 0.641652 | 1_2:-52826467 | Msa0076650:CDS |
| GATCGACGATGACGTTCAGT+GGG | 0.650404 | 1_2:+52826669 | None:intergenic |
| GACGTTCAGTGGGACTAGTG+GGG | 0.659322 | 1_2:+52826679 | None:intergenic |
| GAAACAATCACCACAGCCGT+CGG | 0.679467 | 1_2:+52826547 | None:intergenic |
| GTGGGTGATGGAATTGTCCG+GGG | 0.682973 | 1_2:+52826481 | None:intergenic |
| GGCTGCAACAACAACACCTG+TGG | 0.687479 | 1_2:-52826509 | Msa0076650:CDS |
| CTGAGCGACACCGTGCAGGG+CGG | 0.729304 | 1_2:+52826591 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TTATCAATCCAATCCTCTAA+TGG | - | chr1_2:52826688-52826707 | Msa0076650:CDS | 30.0% | |
| !! | GGTTGATTTGAGCTTTGTAT+TGG | + | chr1_2:52826408-52826427 | None:intergenic | 35.0% |
| CACAATCAACCCATAGAAAT+TGG | + | chr1_2:52826477-52826496 | None:intergenic | 35.0% | |
| ! | TTTTGTGACTGGGAGAACTA+GGG | + | chr1_2:52826368-52826387 | None:intergenic | 40.0% |
| !! | AAGTTGGTGCACATGAGAAT+AGG | + | chr1_2:52826750-52826769 | None:intergenic | 40.0% |
| ! | CTTTTGTGACTGGGAGAACT+AGG | + | chr1_2:52826369-52826388 | None:intergenic | 45.0% |
| GTTCTCCCAGTCACAAAAGA+TGG | - | chr1_2:52826370-52826389 | Msa0076650:CDS | 45.0% | |
| ! | TAGCGCCATCTTTTGTGACT+GGG | + | chr1_2:52826378-52826397 | None:intergenic | 45.0% |
| ATCTCGGTGGCCAATTTCTA+TGG | - | chr1_2:52826464-52826483 | Msa0076650:CDS | 45.0% | |
| TCTCGGTGGCCAATTTCTAT+GGG | - | chr1_2:52826465-52826484 | Msa0076650:CDS | 45.0% | |
| TGTTTCTCATCACCAAAGCC+TGG | - | chr1_2:52826583-52826602 | Msa0076650:CDS | 45.0% | |
| GGTTGAAGCCATTAGAGGAT+TGG | + | chr1_2:52826699-52826718 | None:intergenic | 45.0% | |
| AAACTGCGACATTTCGTCCT+GGG | + | chr1_2:52826720-52826739 | None:intergenic | 45.0% | |
| ! | ACTGGGAGAACTAGGGCTTT+TGG | + | chr1_2:52826361-52826380 | None:intergenic | 50.0% |
| ! | GTAGCGCCATCTTTTGTGAC+TGG | + | chr1_2:52826379-52826398 | None:intergenic | 50.0% |
| ! | TGACGTTCAGTGGGACTAGT+GGG | + | chr1_2:52826438-52826457 | None:intergenic | 50.0% |
| ATGACGTTCAGTGGGACTAG+TGG | + | chr1_2:52826439-52826458 | None:intergenic | 50.0% | |
| GATCGACGATGACGTTCAGT+GGG | + | chr1_2:52826447-52826466 | None:intergenic | 50.0% | |
| AGATCGACGATGACGTTCAG+TGG | + | chr1_2:52826448-52826467 | None:intergenic | 50.0% | |
| CTGAACGTCATCGTCGATCT+CGG | - | chr1_2:52826448-52826467 | Msa0076650:CDS | 50.0% | |
| GAAACAATCACCACAGCCGT+CGG | + | chr1_2:52826569-52826588 | None:intergenic | 50.0% | |
| ! | TTGTTGTTGCAGCCAGGCTT+TGG | + | chr1_2:52826598-52826617 | None:intergenic | 50.0% |
| !! | TGTGGGTGATGGAATTGTCC+GGG | + | chr1_2:52826636-52826655 | None:intergenic | 50.0% |
| !! | GTGTGGGTGATGGAATTGTC+CGG | + | chr1_2:52826637-52826656 | None:intergenic | 50.0% |
| ATCTTCAGCAAGTTCACCGC+TGG | + | chr1_2:52826665-52826684 | None:intergenic | 50.0% | |
| TCCTCTAATGGCTTCAACCC+AGG | - | chr1_2:52826700-52826719 | Msa0076650:CDS | 50.0% | |
| ! | TCCTGGGTTGAAGCCATTAG+AGG | + | chr1_2:52826704-52826723 | None:intergenic | 50.0% |
| GAAACTGCGACATTTCGTCC+TGG | + | chr1_2:52826721-52826740 | None:intergenic | 50.0% | |
| ! | GTGGGACTAGTGGGGTTCTT+TGG | + | chr1_2:52826429-52826448 | None:intergenic | 55.0% |
| ! | GACGTTCAGTGGGACTAGTG+GGG | + | chr1_2:52826437-52826456 | None:intergenic | 55.0% |
| AACGTCATCGTCGATCTCGG+TGG | - | chr1_2:52826451-52826470 | Msa0076650:CDS | 55.0% | |
| !! | GGAATTGGCGAGGGAGCATT+GGG | + | chr1_2:52826548-52826567 | None:intergenic | 55.0% |
| ATCACCACAGCCGTCGGAAT+TGG | + | chr1_2:52826563-52826582 | None:intergenic | 55.0% | |
| !! | CAGGTGTTGTTGTTGCAGCC+AGG | + | chr1_2:52826604-52826623 | None:intergenic | 55.0% |
| GGCTGCAACAACAACACCTG+TGG | - | chr1_2:52826604-52826623 | Msa0076650:CDS | 55.0% | |
| !! | GTGGGTGATGGAATTGTCCG+GGG | + | chr1_2:52826635-52826654 | None:intergenic | 55.0% |
| TTCAGCAAGTTCACCGCTGG+TGG | + | chr1_2:52826662-52826681 | None:intergenic | 55.0% | |
| ! | CGGAATTGGCGAGGGAGCAT+TGG | + | chr1_2:52826549-52826568 | None:intergenic | 60.0% |
| TGCTCCCTCGCCAATTCCGA+CGG | - | chr1_2:52826550-52826569 | Msa0076650:CDS | 60.0% | |
| ACAGCCGTCGGAATTGGCGA+GGG | + | chr1_2:52826557-52826576 | None:intergenic | 60.0% | |
| !! | CAACACCTGTGGTGTCACCC+CGG | - | chr1_2:52826615-52826634 | Msa0076650:intron | 60.0% |
| ATTGTCCGGGGTGACACCAC+AGG | + | chr1_2:52826623-52826642 | None:intergenic | 60.0% | |
| AGCAAGTTCACCGCTGGTGG+CGG | + | chr1_2:52826659-52826678 | None:intergenic | 60.0% | |
| CCTCCACATACCGCCCTGCA+CGG | - | chr1_2:52826512-52826531 | Msa0076650:CDS | 65.0% | |
| ACACCGTGCAGGGCGGTATG+TGG | + | chr1_2:52826518-52826537 | None:intergenic | 65.0% | |
| CACAGCCGTCGGAATTGGCG+AGG | + | chr1_2:52826558-52826577 | None:intergenic | 65.0% | |
| CTCGCCAATTCCGACGGCTG+TGG | - | chr1_2:52826556-52826575 | Msa0076650:CDS | 65.0% | |
| ATCACCCACACCGCCACCAG+CGG | - | chr1_2:52826646-52826665 | Msa0076650:intron | 65.0% | |
| !! | TTCACCGCTGGTGGCGGTGT+GGG | + | chr1_2:52826653-52826672 | None:intergenic | 65.0% |
| CCGTGCAGGGCGGTATGTGG+AGG | + | chr1_2:52826515-52826534 | None:intergenic | 70.0% | |
| CTGAGCGACACCGTGCAGGG+CGG | + | chr1_2:52826525-52826544 | None:intergenic | 70.0% | |
| GGGCTGAGCGACACCGTGCA+GGG | + | chr1_2:52826528-52826547 | None:intergenic | 70.0% | |
| TGGGCTGAGCGACACCGTGC+AGG | + | chr1_2:52826529-52826548 | None:intergenic | 70.0% | |
| !! | GCTGGTGGCGGTGTGGGTGA+TGG | + | chr1_2:52826647-52826666 | None:intergenic | 70.0% |
| !! | GTTCACCGCTGGTGGCGGTG+TGG | + | chr1_2:52826654-52826673 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1_2 | gene | 52826357 | 52826778 | 52826357 | ID=Msa0076650;Name=Msa0076650 |
| chr1_2 | mRNA | 52826357 | 52826778 | 52826357 | ID=Msa0076650-mRNA-1;Parent=Msa0076650;Name=Msa0076650-mRNA-1;_AED=0.43;_eAED=1.00;_QI=0|0|0|1|0|0|2|0|128 |
| chr1_2 | exon | 52826666 | 52826778 | 52826666 | ID=Msa0076650-mRNA-1:exon:16349;Parent=Msa0076650-mRNA-1 |
| chr1_2 | exon | 52826357 | 52826630 | 52826357 | ID=Msa0076650-mRNA-1:exon:16348;Parent=Msa0076650-mRNA-1 |
| chr1_2 | CDS | 52826666 | 52826778 | 52826666 | ID=Msa0076650-mRNA-1:cds;Parent=Msa0076650-mRNA-1 |
| chr1_2 | CDS | 52826357 | 52826630 | 52826357 | ID=Msa0076650-mRNA-1:cds;Parent=Msa0076650-mRNA-1 |
| Gene Sequence |
| Protein sequence |
