AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0329430

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0329430 MtrunA17_Chr2g0311291 95.600 250 8 1 1 250 1 247 3.34e-176 484
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0329430 AT1G79160.1 52.830 265 80 11 1 250 5 239 1.25e-70 217
Msa0329430 AT1G16500.1 45.645 287 91 13 1 250 1 259 2.71e-59 188

Find 58 sgRNAs with CRISPR-Local

Find 220 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATAAATTTAGTTTGGTTTA+AGG 0.123859 2_4:+42853509 Msa0329430:three_prime_UTR
TCAATTTAATAAGTGGTTTA+AGG 0.137788 2_4:+42853412 Msa0329430:exon
CTGAAGTTAGCTCTCTTGTT+AGG 0.176532 2_4:+42851595 Msa0329430:CDS
CTTTCTTCTTCTTGATGATT+TGG 0.191020 2_4:-42853235 None:intergenic
AGCTCTCTTGTTAGGGTTCT+AGG 0.222073 2_4:+42851603 Msa0329430:CDS
ATAAGTGGTTTAAGGATAAT+TGG 0.226069 2_4:+42853420 Msa0329430:exon
AGAATTGGATCTTGATTTAC+AGG 0.260293 2_4:+42851752 Msa0329430:CDS
CTAAATTTGATTCATCAAAT+AGG 0.294108 2_4:-42853027 None:intergenic
TTCAATTGAATCCCAAGAAT+TGG 0.301025 2_4:+42851737 Msa0329430:CDS
AATGATAGATAAATTTAGTT+TGG 0.306367 2_4:+42853502 Msa0329430:three_prime_UTR
AAATTCAAGTCTTGAAGTTT+TGG 0.307088 2_4:-42852974 None:intergenic
ATCCTAGGTTTCTTCATAGT+TGG 0.320748 2_4:-42853376 None:intergenic
GTTCTAGGTGGTGGTGGTGG+TGG 0.321938 2_4:+42851618 Msa0329430:CDS
GTAAATCAAGATCCAATTCT+TGG 0.341520 2_4:-42851749 None:intergenic
GATTCATCAAATAGGTTCAA+TGG 0.342241 2_4:-42853019 None:intergenic
TTTGTTCAGAAATTGGTGAT+TGG 0.359020 2_4:-42852928 None:intergenic
GGAACATTTGTTTGTGTTGA+TGG 0.359845 2_4:-42852998 None:intergenic
TTGTTCAGAAATTGGTGATT+GGG 0.387101 2_4:-42852927 None:intergenic
GGTTTCTTCATAGTTGGAAT+TGG 0.391404 2_4:-42853370 None:intergenic
GAGAGAGAAAATAGAGATAG+AGG 0.402785 2_4:-42851533 None:intergenic
ATTTACAGGTTCCTAGTGGC+TGG 0.409368 2_4:+42851766 Msa0329430:CDS
TAATATTTCAATTTAATAAG+TGG 0.409427 2_4:+42853405 Msa0329430:exon
TGAAGTTAGCTCTCTTGTTA+GGG 0.437373 2_4:+42851596 Msa0329430:CDS
ACACTTTGGTAATTGCTTGT+TGG 0.440772 2_4:-42853076 None:intergenic
AGGGTTCTAGGTGGTGGTGG+TGG 0.444252 2_4:+42851615 Msa0329430:CDS
CCAATGGCTGCAGGGTGTCC+TGG 0.445132 2_4:+42853283 Msa0329430:CDS
TAAATCAAGATCCAATTCTT+GGG 0.456351 2_4:-42851748 None:intergenic
CTTGTTAGGGTTCTAGGTGG+TGG 0.457284 2_4:+42851609 Msa0329430:CDS
ATTTCATCACCAATGGCTGC+AGG 0.458385 2_4:+42853274 Msa0329430:CDS
ATTTAGTTTGGTTTAAGGAT+AGG 0.463760 2_4:+42853514 Msa0329430:three_prime_UTR
GTTAGGGTTCTAGGTGGTGG+TGG 0.474763 2_4:+42851612 Msa0329430:CDS
AGAATAATCCTAAGTGTCCT+AGG 0.485771 2_4:+42853332 Msa0329430:CDS
GAAATATTAAGATCAATCCT+AGG 0.490407 2_4:-42853391 None:intergenic
CCAGGACACCCTGCAGCCAT+TGG 0.515852 2_4:-42853283 None:intergenic
GAACATTTGTTTGTGTTGAT+GGG 0.520707 2_4:-42852997 None:intergenic
GAGTTACACCTAGGACACTT+AGG 0.522057 2_4:-42853340 None:intergenic
TGTCAAGAGTGCATACACTT+TGG 0.525717 2_4:-42853090 None:intergenic
TTCTTGGGATTCAATTGAAG+AGG 0.536073 2_4:-42851733 None:intergenic
ATAGAGGAAGAAGATGAGAA+AGG 0.538057 2_4:-42851517 None:intergenic
ATTACAAGAGACTTGCTTGG+TGG 0.546683 2_4:+42851708 Msa0329430:CDS
TTCATTGAGAAACTAAGCAA+TGG 0.551761 2_4:+42851569 Msa0329430:exon
TTTCAATTTACATTAAACCC+TGG 0.552076 2_4:-42853559 None:intergenic
GAAAATGATTTCATCACCAA+TGG 0.554457 2_4:+42853267 Msa0329430:CDS
CTGATTACAAGAGACTTGCT+TGG 0.555890 2_4:+42851705 Msa0329430:CDS
TTACAAGAGACTTGCTTGGT+GGG 0.576143 2_4:+42851709 Msa0329430:CDS
TTTACAGGTTCCTAGTGGCT+GGG 0.577425 2_4:+42851767 Msa0329430:CDS
GAAGAGCAACATCGAACGGT+AGG 0.589713 2_4:+42851651 Msa0329430:CDS
CTTGATTTACAGGTTCCTAG+TGG 0.589849 2_4:+42851762 Msa0329430:CDS
GATGAAGAAGAAGAATATGA+AGG 0.593006 2_4:-42853199 None:intergenic
TTTGAAGGGGATATTGGCCA+GGG 0.600705 2_4:+42853542 Msa0329430:three_prime_UTR
CAAAGAAGAGCAACATCGAA+CGG 0.609358 2_4:+42851647 Msa0329430:CDS
ACGGTAGGTAATGATTCTCA+TGG 0.626022 2_4:+42851666 Msa0329430:CDS
TTTCATCACCAATGGCTGCA+GGG 0.626120 2_4:+42853275 Msa0329430:CDS
TTCCAACTATGAAGAAACCT+AGG 0.628247 2_4:+42853374 Msa0329430:CDS
TTCTCATGGTGAGAAATCAA+CGG 0.650661 2_4:+42851680 Msa0329430:CDS
AATACATAAGATAAGCAACC+AGG 0.660615 2_4:-42853301 None:intergenic
TCTCTTGTTAGGGTTCTAGG+TGG 0.667514 2_4:+42851606 Msa0329430:CDS
GGTACAATAGAGTTACACCT+AGG 0.708224 2_4:-42853349 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTAAAATTCAATTTGTTT+AGG + chr2_4:42852256-42852275 Msa0329430:intron 10.0%
!! CTAAATGAAATAAAATTAAA+TGG - chr2_4:42852612-42852631 None:intergenic 10.0%
!! TAATATTTCAATTTAATAAG+TGG + chr2_4:42853405-42853424 Msa0329430:exon 10.0%
!!! ATTTAAAATTCAATTTGTTT+AGG + chr2_4:42852256-42852275 Msa0329430:intron 10.0%
!! CTAAATGAAATAAAATTAAA+TGG - chr2_4:42852612-42852631 None:intergenic 10.0%
!! TAATATTTCAATTTAATAAG+TGG + chr2_4:42853405-42853424 Msa0329430:exon 10.0%
!!! GAAAATATAAATTTTCTCTT+TGG + chr2_4:42851834-42851853 Msa0329430:intron 15.0%
!! TATGTTTACTTATGATTATT+AGG + chr2_4:42851890-42851909 Msa0329430:intron 15.0%
!! AACTTAGATATCAAAAAATT+TGG - chr2_4:42852482-42852501 None:intergenic 15.0%
!! TATCTAAGTTAATGAAAATA+TGG + chr2_4:42852492-42852511 Msa0329430:intron 15.0%
!! AGTCTTTATGATATATTTAT+GGG - chr2_4:42852561-42852580 None:intergenic 15.0%
!! AAGTCTTTATGATATATTTA+TGG - chr2_4:42852562-42852581 None:intergenic 15.0%
!!! ACCAATTTACATTTTTATTA+CGG + chr2_4:42852741-42852760 Msa0329430:intron 15.0%
!! AATGATAGATAAATTTAGTT+TGG + chr2_4:42853502-42853521 Msa0329430:three_prime_UTR 15.0%
!!! GAAAATATAAATTTTCTCTT+TGG + chr2_4:42851834-42851853 Msa0329430:intron 15.0%
!! TATGTTTACTTATGATTATT+AGG + chr2_4:42851890-42851909 Msa0329430:intron 15.0%
!! AACTTAGATATCAAAAAATT+TGG - chr2_4:42852482-42852501 None:intergenic 15.0%
!! TATCTAAGTTAATGAAAATA+TGG + chr2_4:42852492-42852511 Msa0329430:intron 15.0%
!! AGTCTTTATGATATATTTAT+GGG - chr2_4:42852561-42852580 None:intergenic 15.0%
!! AAGTCTTTATGATATATTTA+TGG - chr2_4:42852562-42852581 None:intergenic 15.0%
!!! ACCAATTTACATTTTTATTA+CGG + chr2_4:42852741-42852760 Msa0329430:intron 15.0%
!! AATGATAGATAAATTTAGTT+TGG + chr2_4:42853502-42853521 Msa0329430:three_prime_UTR 15.0%
!!! TATATTTTCTTTTTGTTTCC+CGG + chr2_4:42851811-42851830 Msa0329430:intron 20.0%
!!! AGAGAAAATTTATATTTTCC+CGG - chr2_4:42851833-42851852 None:intergenic 20.0%
!!! TGTTTGTGTTTTTTTTACAA+TGG + chr2_4:42851978-42851997 Msa0329430:intron 20.0%
!!! TCTTTTTGATTTTTTACATG+AGG + chr2_4:42852084-42852103 Msa0329430:intron 20.0%
!!! TTTTGTTTTGCTATTTTGAT+TGG + chr2_4:42852162-42852181 Msa0329430:intron 20.0%
!! TATGATATATTTATGGGTAA+GGG - chr2_4:42852555-42852574 None:intergenic 20.0%
!! TTATGATATATTTATGGGTA+AGG - chr2_4:42852556-42852575 None:intergenic 20.0%
!! ACCGTAATAAAAATGTAAAT+TGG - chr2_4:42852745-42852764 None:intergenic 20.0%
!!! GTAAAACACTTATTTTTACT+TGG - chr2_4:42852836-42852855 None:intergenic 20.0%
!!! TGATTTTTTTGTTCAGAAAT+TGG - chr2_4:42852938-42852957 None:intergenic 20.0%
!! ATTTCTGAACAAAAAAATCA+AGG + chr2_4:42852938-42852957 Msa0329430:CDS 20.0%
!! CTAAATTTGATTCATCAAAT+AGG - chr2_4:42853030-42853049 None:intergenic 20.0%
!! TCAATTTAATAAGTGGTTTA+AGG + chr2_4:42853412-42853431 Msa0329430:exon 20.0%
!!! GATAAATTTAGTTTGGTTTA+AGG + chr2_4:42853509-42853528 Msa0329430:three_prime_UTR 20.0%
!!! TATATTTTCTTTTTGTTTCC+CGG + chr2_4:42851811-42851830 Msa0329430:intron 20.0%
!!! AGAGAAAATTTATATTTTCC+CGG - chr2_4:42851833-42851852 None:intergenic 20.0%
!!! TGTTTGTGTTTTTTTTACAA+TGG + chr2_4:42851978-42851997 Msa0329430:intron 20.0%
!!! TCTTTTTGATTTTTTACATG+AGG + chr2_4:42852084-42852103 Msa0329430:intron 20.0%
!!! TTTTGTTTTGCTATTTTGAT+TGG + chr2_4:42852162-42852181 Msa0329430:intron 20.0%
!! TATGATATATTTATGGGTAA+GGG - chr2_4:42852555-42852574 None:intergenic 20.0%
!! TTATGATATATTTATGGGTA+AGG - chr2_4:42852556-42852575 None:intergenic 20.0%
!! ACCGTAATAAAAATGTAAAT+TGG - chr2_4:42852745-42852764 None:intergenic 20.0%
!!! GTAAAACACTTATTTTTACT+TGG - chr2_4:42852836-42852855 None:intergenic 20.0%
!!! TGATTTTTTTGTTCAGAAAT+TGG - chr2_4:42852938-42852957 None:intergenic 20.0%
!! ATTTCTGAACAAAAAAATCA+AGG + chr2_4:42852938-42852957 Msa0329430:CDS 20.0%
!! CTAAATTTGATTCATCAAAT+AGG - chr2_4:42853030-42853049 None:intergenic 20.0%
!! TCAATTTAATAAGTGGTTTA+AGG + chr2_4:42853412-42853431 Msa0329430:exon 20.0%
!!! GATAAATTTAGTTTGGTTTA+AGG + chr2_4:42853509-42853528 Msa0329430:three_prime_UTR 20.0%
! TAAATCAAGATCCAATTCTT+GGG - chr2_4:42851751-42851770 None:intergenic 25.0%
! CAAAAAGAAAATATACCTGA+AGG - chr2_4:42851806-42851825 None:intergenic 25.0%
!!! ATATTTTCTTTTTGTTTCCC+GGG + chr2_4:42851812-42851831 Msa0329430:intron 25.0%
!! GAGAAAATTTATATTTTCCC+GGG - chr2_4:42851832-42851851 None:intergenic 25.0%
!!! CAGATTTTTTATGTTGTCTA+TGG + chr2_4:42852110-42852129 Msa0329430:intron 25.0%
! TTGTTTGTGTTTCATTTGTA+TGG + chr2_4:42852333-42852352 Msa0329430:intron 25.0%
! ATGATATATTTATGGGTAAG+GGG - chr2_4:42852554-42852573 None:intergenic 25.0%
!!! ATTTTGTTTTTTATGCAGTC+AGG + chr2_4:42852872-42852891 Msa0329430:intron 25.0%
!!! TTTTGTTTTTTATGCAGTCA+GGG + chr2_4:42852873-42852892 Msa0329430:intron 25.0%
!! AAATTCAAGTCTTGAAGTTT+TGG - chr2_4:42852977-42852996 None:intergenic 25.0%
! GAAATATTAAGATCAATCCT+AGG - chr2_4:42853394-42853413 None:intergenic 25.0%
! ATAAGTGGTTTAAGGATAAT+TGG + chr2_4:42853420-42853439 Msa0329430:exon 25.0%
!!! ATTTAGTTTGGTTTAAGGAT+AGG + chr2_4:42853514-42853533 Msa0329430:three_prime_UTR 25.0%
! TTTCAATTTACATTAAACCC+TGG - chr2_4:42853562-42853581 None:intergenic 25.0%
! TAAATCAAGATCCAATTCTT+GGG - chr2_4:42851751-42851770 None:intergenic 25.0%
! CAAAAAGAAAATATACCTGA+AGG - chr2_4:42851806-42851825 None:intergenic 25.0%
!!! ATATTTTCTTTTTGTTTCCC+GGG + chr2_4:42851812-42851831 Msa0329430:intron 25.0%
!! GAGAAAATTTATATTTTCCC+GGG - chr2_4:42851832-42851851 None:intergenic 25.0%
!!! CAGATTTTTTATGTTGTCTA+TGG + chr2_4:42852110-42852129 Msa0329430:intron 25.0%
! TTGTTTGTGTTTCATTTGTA+TGG + chr2_4:42852333-42852352 Msa0329430:intron 25.0%
! ATGATATATTTATGGGTAAG+GGG - chr2_4:42852554-42852573 None:intergenic 25.0%
!!! ATTTTGTTTTTTATGCAGTC+AGG + chr2_4:42852872-42852891 Msa0329430:intron 25.0%
!!! TTTTGTTTTTTATGCAGTCA+GGG + chr2_4:42852873-42852892 Msa0329430:intron 25.0%
!! AAATTCAAGTCTTGAAGTTT+TGG - chr2_4:42852977-42852996 None:intergenic 25.0%
! GAAATATTAAGATCAATCCT+AGG - chr2_4:42853394-42853413 None:intergenic 25.0%
! ATAAGTGGTTTAAGGATAAT+TGG + chr2_4:42853420-42853439 Msa0329430:exon 25.0%
!!! ATTTAGTTTGGTTTAAGGAT+AGG + chr2_4:42853514-42853533 Msa0329430:three_prime_UTR 25.0%
! TTTCAATTTACATTAAACCC+TGG - chr2_4:42853562-42853581 None:intergenic 25.0%
TTCATTGAGAAACTAAGCAA+TGG + chr2_4:42851569-42851588 Msa0329430:exon 30.0%
TTCAATTGAATCCCAAGAAT+TGG + chr2_4:42851737-42851756 Msa0329430:CDS 30.0%
GTAAATCAAGATCCAATTCT+TGG - chr2_4:42851752-42851771 None:intergenic 30.0%
AGAATTGGATCTTGATTTAC+AGG + chr2_4:42851752-42851771 Msa0329430:CDS 30.0%
!! TTGCATTTTAATGTAACAGC+AGG + chr2_4:42852373-42852392 Msa0329430:intron 30.0%
GACATAAATACAGAGTACAT+AGG - chr2_4:42852423-42852442 None:intergenic 30.0%
! TTGTTCAGAAATTGGTGATT+GGG - chr2_4:42852930-42852949 None:intergenic 30.0%
! TTTGTTCAGAAATTGGTGAT+TGG - chr2_4:42852931-42852950 None:intergenic 30.0%
! GAACATTTGTTTGTGTTGAT+GGG - chr2_4:42853000-42853019 None:intergenic 30.0%
GATTCATCAAATAGGTTCAA+TGG - chr2_4:42853022-42853041 None:intergenic 30.0%
! AAGCAGATGAAACAAGTTTT+AGG - chr2_4:42853054-42853073 None:intergenic 30.0%
AAAAAGAACCCATAAGGAAA+AGG + chr2_4:42853140-42853159 Msa0329430:CDS 30.0%
! AATGATGACCTTTTCCTTAT+GGG - chr2_4:42853151-42853170 None:intergenic 30.0%
GATGAAGAAGAAGAATATGA+AGG - chr2_4:42853202-42853221 None:intergenic 30.0%
! CTTTCTTCTTCTTGATGATT+TGG - chr2_4:42853238-42853257 None:intergenic 30.0%
GAAAATGATTTCATCACCAA+TGG + chr2_4:42853267-42853286 Msa0329430:CDS 30.0%
AATACATAAGATAAGCAACC+AGG - chr2_4:42853304-42853323 None:intergenic 30.0%
! TAAGGATAGGATCATTTTGA+AGG + chr2_4:42853527-42853546 Msa0329430:three_prime_UTR 30.0%
! AAGGATAGGATCATTTTGAA+GGG + chr2_4:42853528-42853547 Msa0329430:three_prime_UTR 30.0%
TTCATTGAGAAACTAAGCAA+TGG + chr2_4:42851569-42851588 Msa0329430:exon 30.0%
TTCAATTGAATCCCAAGAAT+TGG + chr2_4:42851737-42851756 Msa0329430:CDS 30.0%
GTAAATCAAGATCCAATTCT+TGG - chr2_4:42851752-42851771 None:intergenic 30.0%
AGAATTGGATCTTGATTTAC+AGG + chr2_4:42851752-42851771 Msa0329430:CDS 30.0%
!! TTGCATTTTAATGTAACAGC+AGG + chr2_4:42852373-42852392 Msa0329430:intron 30.0%
GACATAAATACAGAGTACAT+AGG - chr2_4:42852423-42852442 None:intergenic 30.0%
! TTGTTCAGAAATTGGTGATT+GGG - chr2_4:42852930-42852949 None:intergenic 30.0%
! TTTGTTCAGAAATTGGTGAT+TGG - chr2_4:42852931-42852950 None:intergenic 30.0%
! GAACATTTGTTTGTGTTGAT+GGG - chr2_4:42853000-42853019 None:intergenic 30.0%
GATTCATCAAATAGGTTCAA+TGG - chr2_4:42853022-42853041 None:intergenic 30.0%
! AAGCAGATGAAACAAGTTTT+AGG - chr2_4:42853054-42853073 None:intergenic 30.0%
AAAAAGAACCCATAAGGAAA+AGG + chr2_4:42853140-42853159 Msa0329430:CDS 30.0%
! AATGATGACCTTTTCCTTAT+GGG - chr2_4:42853151-42853170 None:intergenic 30.0%
GATGAAGAAGAAGAATATGA+AGG - chr2_4:42853202-42853221 None:intergenic 30.0%
! CTTTCTTCTTCTTGATGATT+TGG - chr2_4:42853238-42853257 None:intergenic 30.0%
GAAAATGATTTCATCACCAA+TGG + chr2_4:42853267-42853286 Msa0329430:CDS 30.0%
AATACATAAGATAAGCAACC+AGG - chr2_4:42853304-42853323 None:intergenic 30.0%
! TAAGGATAGGATCATTTTGA+AGG + chr2_4:42853527-42853546 Msa0329430:three_prime_UTR 30.0%
! AAGGATAGGATCATTTTGAA+GGG + chr2_4:42853528-42853547 Msa0329430:three_prime_UTR 30.0%
ATAGAGGAAGAAGATGAGAA+AGG - chr2_4:42851520-42851539 None:intergenic 35.0%
GAGAGAGAAAATAGAGATAG+AGG - chr2_4:42851536-42851555 None:intergenic 35.0%
! TGAAGTTAGCTCTCTTGTTA+GGG + chr2_4:42851596-42851615 Msa0329430:CDS 35.0%
TTCTCATGGTGAGAAATCAA+CGG + chr2_4:42851680-42851699 Msa0329430:CDS 35.0%
TTCTTGGGATTCAATTGAAG+AGG - chr2_4:42851736-42851755 None:intergenic 35.0%
GATGCATAGTTCTTCAATCA+TGG + chr2_4:42852009-42852028 Msa0329430:intron 35.0%
ATATATTTATGGGTAAGGGG+CGG - chr2_4:42852551-42852570 None:intergenic 35.0%
TAATCTTTGCTTATGCGTAG+AGG - chr2_4:42852808-42852827 None:intergenic 35.0%
GGAACATTTGTTTGTGTTGA+TGG - chr2_4:42853001-42853020 None:intergenic 35.0%
! ACACTTTGGTAATTGCTTGT+TGG - chr2_4:42853079-42853098 None:intergenic 35.0%
! GAATGATGACCTTTTCCTTA+TGG - chr2_4:42853152-42853171 None:intergenic 35.0%
AGAATAATCCTAAGTGTCCT+AGG + chr2_4:42853332-42853351 Msa0329430:CDS 35.0%
GGTTTCTTCATAGTTGGAAT+TGG - chr2_4:42853373-42853392 None:intergenic 35.0%
TTCCAACTATGAAGAAACCT+AGG + chr2_4:42853374-42853393 Msa0329430:CDS 35.0%
ATCCTAGGTTTCTTCATAGT+TGG - chr2_4:42853379-42853398 None:intergenic 35.0%
!! AGGATAGGATCATTTTGAAG+GGG + chr2_4:42853529-42853548 Msa0329430:three_prime_UTR 35.0%
!! GATCATTTTGAAGGGGATAT+TGG + chr2_4:42853536-42853555 Msa0329430:three_prime_UTR 35.0%
ATAGAGGAAGAAGATGAGAA+AGG - chr2_4:42851520-42851539 None:intergenic 35.0%
GAGAGAGAAAATAGAGATAG+AGG - chr2_4:42851536-42851555 None:intergenic 35.0%
! TGAAGTTAGCTCTCTTGTTA+GGG + chr2_4:42851596-42851615 Msa0329430:CDS 35.0%
TTCTCATGGTGAGAAATCAA+CGG + chr2_4:42851680-42851699 Msa0329430:CDS 35.0%
TTCTTGGGATTCAATTGAAG+AGG - chr2_4:42851736-42851755 None:intergenic 35.0%
GATGCATAGTTCTTCAATCA+TGG + chr2_4:42852009-42852028 Msa0329430:intron 35.0%
ATATATTTATGGGTAAGGGG+CGG - chr2_4:42852551-42852570 None:intergenic 35.0%
TAATCTTTGCTTATGCGTAG+AGG - chr2_4:42852808-42852827 None:intergenic 35.0%
GGAACATTTGTTTGTGTTGA+TGG - chr2_4:42853001-42853020 None:intergenic 35.0%
! ACACTTTGGTAATTGCTTGT+TGG - chr2_4:42853079-42853098 None:intergenic 35.0%
! GAATGATGACCTTTTCCTTA+TGG - chr2_4:42853152-42853171 None:intergenic 35.0%
AGAATAATCCTAAGTGTCCT+AGG + chr2_4:42853332-42853351 Msa0329430:CDS 35.0%
GGTTTCTTCATAGTTGGAAT+TGG - chr2_4:42853373-42853392 None:intergenic 35.0%
TTCCAACTATGAAGAAACCT+AGG + chr2_4:42853374-42853393 Msa0329430:CDS 35.0%
ATCCTAGGTTTCTTCATAGT+TGG - chr2_4:42853379-42853398 None:intergenic 35.0%
!! AGGATAGGATCATTTTGAAG+GGG + chr2_4:42853529-42853548 Msa0329430:three_prime_UTR 35.0%
!! GATCATTTTGAAGGGGATAT+TGG + chr2_4:42853536-42853555 Msa0329430:three_prime_UTR 35.0%
! CTGAAGTTAGCTCTCTTGTT+AGG + chr2_4:42851595-42851614 Msa0329430:CDS 40.0%
CAAAGAAGAGCAACATCGAA+CGG + chr2_4:42851647-42851666 Msa0329430:CDS 40.0%
ACGGTAGGTAATGATTCTCA+TGG + chr2_4:42851666-42851685 Msa0329430:CDS 40.0%
! CTGATTACAAGAGACTTGCT+TGG + chr2_4:42851705-42851724 Msa0329430:CDS 40.0%
! ATTACAAGAGACTTGCTTGG+TGG + chr2_4:42851708-42851727 Msa0329430:CDS 40.0%
! TTACAAGAGACTTGCTTGGT+GGG + chr2_4:42851709-42851728 Msa0329430:CDS 40.0%
CTTGATTTACAGGTTCCTAG+TGG + chr2_4:42851762-42851781 Msa0329430:CDS 40.0%
ATCTGATCAAAATGGCGTCA+TGG - chr2_4:42852521-42852540 None:intergenic 40.0%
GTGGAGCAATCTGATCAAAA+TGG - chr2_4:42852529-42852548 None:intergenic 40.0%
! TGTCAAGAGTGCATACACTT+TGG - chr2_4:42853093-42853112 None:intergenic 40.0%
GAGCTGAAAAAGAACCCATA+AGG + chr2_4:42853134-42853153 Msa0329430:CDS 40.0%
GGTACAATAGAGTTACACCT+AGG - chr2_4:42853352-42853371 None:intergenic 40.0%
! CTGAAGTTAGCTCTCTTGTT+AGG + chr2_4:42851595-42851614 Msa0329430:CDS 40.0%
CAAAGAAGAGCAACATCGAA+CGG + chr2_4:42851647-42851666 Msa0329430:CDS 40.0%
ACGGTAGGTAATGATTCTCA+TGG + chr2_4:42851666-42851685 Msa0329430:CDS 40.0%
! CTGATTACAAGAGACTTGCT+TGG + chr2_4:42851705-42851724 Msa0329430:CDS 40.0%
! ATTACAAGAGACTTGCTTGG+TGG + chr2_4:42851708-42851727 Msa0329430:CDS 40.0%
! TTACAAGAGACTTGCTTGGT+GGG + chr2_4:42851709-42851728 Msa0329430:CDS 40.0%
CTTGATTTACAGGTTCCTAG+TGG + chr2_4:42851762-42851781 Msa0329430:CDS 40.0%
ATCTGATCAAAATGGCGTCA+TGG - chr2_4:42852521-42852540 None:intergenic 40.0%
GTGGAGCAATCTGATCAAAA+TGG - chr2_4:42852529-42852548 None:intergenic 40.0%
! TGTCAAGAGTGCATACACTT+TGG - chr2_4:42853093-42853112 None:intergenic 40.0%
GAGCTGAAAAAGAACCCATA+AGG + chr2_4:42853134-42853153 Msa0329430:CDS 40.0%
GGTACAATAGAGTTACACCT+AGG - chr2_4:42853352-42853371 None:intergenic 40.0%
AGCTCTCTTGTTAGGGTTCT+AGG + chr2_4:42851603-42851622 Msa0329430:CDS 45.0%
!! TCTCTTGTTAGGGTTCTAGG+TGG + chr2_4:42851606-42851625 Msa0329430:CDS 45.0%
ATTTACAGGTTCCTAGTGGC+TGG + chr2_4:42851766-42851785 Msa0329430:CDS 45.0%
TTTACAGGTTCCTAGTGGCT+GGG + chr2_4:42851767-42851786 Msa0329430:CDS 45.0%
GGAAAAACGTCTTGACCTTC+AGG + chr2_4:42851788-42851807 Msa0329430:CDS 45.0%
ACGAAAACTCGAGTAACTGC+AGG - chr2_4:42852399-42852418 None:intergenic 45.0%
ATTTCATCACCAATGGCTGC+AGG + chr2_4:42853274-42853293 Msa0329430:CDS 45.0%
TTTCATCACCAATGGCTGCA+GGG + chr2_4:42853275-42853294 Msa0329430:CDS 45.0%
GAGTTACACCTAGGACACTT+AGG - chr2_4:42853343-42853362 None:intergenic 45.0%
!! TTTTGAAGGGGATATTGGCC+AGG + chr2_4:42853541-42853560 Msa0329430:three_prime_UTR 45.0%
TTTGAAGGGGATATTGGCCA+GGG + chr2_4:42853542-42853561 Msa0329430:three_prime_UTR 45.0%
AGCTCTCTTGTTAGGGTTCT+AGG + chr2_4:42851603-42851622 Msa0329430:CDS 45.0%
!! TCTCTTGTTAGGGTTCTAGG+TGG + chr2_4:42851606-42851625 Msa0329430:CDS 45.0%
ATTTACAGGTTCCTAGTGGC+TGG + chr2_4:42851766-42851785 Msa0329430:CDS 45.0%
TTTACAGGTTCCTAGTGGCT+GGG + chr2_4:42851767-42851786 Msa0329430:CDS 45.0%
GGAAAAACGTCTTGACCTTC+AGG + chr2_4:42851788-42851807 Msa0329430:CDS 45.0%
ACGAAAACTCGAGTAACTGC+AGG - chr2_4:42852399-42852418 None:intergenic 45.0%
ATTTCATCACCAATGGCTGC+AGG + chr2_4:42853274-42853293 Msa0329430:CDS 45.0%
TTTCATCACCAATGGCTGCA+GGG + chr2_4:42853275-42853294 Msa0329430:CDS 45.0%
GAGTTACACCTAGGACACTT+AGG - chr2_4:42853343-42853362 None:intergenic 45.0%
!! TTTTGAAGGGGATATTGGCC+AGG + chr2_4:42853541-42853560 Msa0329430:three_prime_UTR 45.0%
TTTGAAGGGGATATTGGCCA+GGG + chr2_4:42853542-42853561 Msa0329430:three_prime_UTR 45.0%
!! AAAAATTATATTAAAAATGT+AGG - chr2_4:42853460-42853479 None:intergenic 5.0%
!! AAAAATTATATTAAAAATGT+AGG - chr2_4:42853460-42853479 None:intergenic 5.0%
!! CTTGTTAGGGTTCTAGGTGG+TGG + chr2_4:42851609-42851628 Msa0329430:CDS 50.0%
GAAGAGCAACATCGAACGGT+AGG + chr2_4:42851651-42851670 Msa0329430:CDS 50.0%
! AGACGTTTTTCCCAGCCACT+AGG - chr2_4:42851780-42851799 None:intergenic 50.0%
TATTTATGGGTAAGGGGCGG+TGG - chr2_4:42852548-42852567 None:intergenic 50.0%
! GCGTAGAGGCATATGAGTAC+TGG - chr2_4:42852794-42852813 None:intergenic 50.0%
!! CTTGTTAGGGTTCTAGGTGG+TGG + chr2_4:42851609-42851628 Msa0329430:CDS 50.0%
GAAGAGCAACATCGAACGGT+AGG + chr2_4:42851651-42851670 Msa0329430:CDS 50.0%
! AGACGTTTTTCCCAGCCACT+AGG - chr2_4:42851780-42851799 None:intergenic 50.0%
TATTTATGGGTAAGGGGCGG+TGG - chr2_4:42852548-42852567 None:intergenic 50.0%
! GCGTAGAGGCATATGAGTAC+TGG - chr2_4:42852794-42852813 None:intergenic 50.0%
!! GTTAGGGTTCTAGGTGGTGG+TGG + chr2_4:42851612-42851631 Msa0329430:CDS 55.0%
!! GTTAGGGTTCTAGGTGGTGG+TGG + chr2_4:42851612-42851631 Msa0329430:CDS 55.0%
!! AGGGTTCTAGGTGGTGGTGG+TGG + chr2_4:42851615-42851634 Msa0329430:CDS 60.0%
!! GTTCTAGGTGGTGGTGGTGG+TGG + chr2_4:42851618-42851637 Msa0329430:CDS 60.0%
!! AGGGTTCTAGGTGGTGGTGG+TGG + chr2_4:42851615-42851634 Msa0329430:CDS 60.0%
!! GTTCTAGGTGGTGGTGGTGG+TGG + chr2_4:42851618-42851637 Msa0329430:CDS 60.0%
!! CTAGGTGGTGGTGGTGGTGG+TGG + chr2_4:42851621-42851640 Msa0329430:CDS 65.0%
CCAGGACACCCTGCAGCCAT+TGG - chr2_4:42853286-42853305 None:intergenic 65.0%
! CCAATGGCTGCAGGGTGTCC+TGG + chr2_4:42853283-42853302 Msa0329430:CDS 65.0%
!! CTAGGTGGTGGTGGTGGTGG+TGG + chr2_4:42851621-42851640 Msa0329430:CDS 65.0%
CCAGGACACCCTGCAGCCAT+TGG - chr2_4:42853286-42853305 None:intergenic 65.0%
! CCAATGGCTGCAGGGTGTCC+TGG + chr2_4:42853283-42853302 Msa0329430:CDS 65.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 42851513 42853586 42851513 ID=Msa0329430;Name=Msa0329430
chr2_4 mRNA 42851513 42853586 42851513 ID=Msa0329430-mRNA-1;Parent=Msa0329430;Name=Msa0329430-mRNA-1;_AED=0.02;_eAED=0.02;_QI=75|1|1|1|1|1|2|166|250
chr2_4 exon 42851513 42851809 42851513 ID=Msa0329430-mRNA-1:exon:13161;Parent=Msa0329430-mRNA-1
chr2_4 exon 42852890 42853586 42852890 ID=Msa0329430-mRNA-1:exon:13162;Parent=Msa0329430-mRNA-1
chr2_4 five_prime_UTR 42851513 42851587 42851513 ID=Msa0329430-mRNA-1:five_prime_utr;Parent=Msa0329430-mRNA-1
chr2_4 CDS 42851588 42851809 42851588 ID=Msa0329430-mRNA-1:cds;Parent=Msa0329430-mRNA-1
chr2_4 CDS 42852890 42853420 42852890 ID=Msa0329430-mRNA-1:cds;Parent=Msa0329430-mRNA-1
chr2_4 three_prime_UTR 42853421 42853586 42853421 ID=Msa0329430-mRNA-1:three_prime_utr;Parent=Msa0329430-mRNA-1
Gene Sequence

>Msa0329430

ATGGCTGCTGAAGTTAGCTCTCTTGTTAGGGTTCTAGGTGGTGGTGGTGGTGGTGGATACAAAGAAGAGCAACATCGAACGGTAGGTAATGATTCTCATGGTGAGAAATCAACGGCTCTGATTACAAGAGACTTGCTTGGTGGGTCCTCTTCAATTGAATCCCAAGAATTGGATCTTGATTTACAGGTTCCTAGTGGCTGGGAAAAACGTCTTGACCTTCAGTCAGGGAAAGTTTATATTCAAAGATGCAACACATTAACCCAATCACCAATTTCTGAACAAAAAAATCAAGGAAAATCAACAACACCAAAACTTCAAGACTTGAATTTCCCATCAACACAAACAAATGTTCCATTGAACCTATTTGATGAATCAAATTTAGACCTAAAACTTGTTTCATCTGCTTTACCAACAAGCAATTACCAAAGTGTATGCACTCTTGACAAAGTGAAATCAGCACTTGAAAGAGCTGAAAAAGAACCCATAAGGAAAAGGTCATCATTCTTGAAATCGTCGATTTCAGCAACATCGCCTTCATATTCTTCTTCTTCATCGTCGTTAAGAGAACCAAATCATCAAGAAGAAGAAAGTGATCAAGAGAAAATGATTTCATCACCAATGGCTGCAGGGTGTCCTGGTTGCTTATCTTATGTATTGATTATGAAGAATAATCCTAAGTGTCCTAGGTGTAACTCTATTGTACCAATTCCAACTATGAAGAAACCTAGGATTGATCTTAATATTTCAATTTAA
Protein sequence

>Msa0329430

MAAEVSSLVRVLGGGGGGGYKEEQHRTVGNDSHGEKSTALITRDLLGGSSSIESQELDLDLQVPSGWEKRLDLQSGKVYIQRCNTLTQSPISEQKNQGKSTTPKLQDLNFPSTQTNVPLNLFDESNLDLKLVSSALPTSNYQSVCTLDKVKSALERAEKEPIRKRSSFLKSSISATSPSYSSSSSSLREPNHQEEESDQEKMISSPMAAGCPGCLSYVLIMKNNPKCPRCNSIVPIPTMKKPRIDLNISI*