AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0678380

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0678380 MtrunA17_Chr4g0023621 97.500 160 4 0 1 160 1 160 1.57e-112 316
Msa0678380 MtrunA17_Chr2g0323081 71.519 158 45 0 1 158 1 158 1.65e-80 235
Msa0678380 MtrunA17_Chr4g0049001 55.696 158 70 0 1 158 1 158 9.91e-68 202
Msa0678380 MtrunA17_Chr2g0280491 55.063 158 71 0 1 158 1 158 2.90e-59 182
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0678380 AT2G32280.1 63.750 160 58 0 1 160 1 160 1.39e-77 228
Msa0678380 AT2G32280.2 63.750 160 58 0 1 160 1 160 1.39e-77 228
Msa0678380 AT4G21310.1 63.291 158 58 0 1 158 1 158 1.81e-72 215
Msa0678380 AT4G21310.2 60.000 120 48 0 39 158 1 120 1.37e-51 161
Msa0678380 AT1G11500.1 39.375 160 90 1 6 158 6 165 1.43e-33 117
Msa0678380 AT1G11500.3 39.375 160 90 1 6 158 11 170 2.17e-33 117
Msa0678380 AT1G11500.2 42.105 114 66 0 45 158 24 137 2.14e-27 100
Msa0678380 AT1G05291.1 40.000 145 80 3 12 153 23 163 6.62e-24 92.8

Find 61 sgRNAs with CRISPR-Local

Find 270 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCTATTATGGCCCCTGTTT+TGG 0.142899 4_4:-34056869 Msa0678380:three_prime_UTR
GTTTCAAAATGTTGCGATTT+TGG 0.145111 4_4:-34057002 Msa0678380:three_prime_UTR
TTCTCAACAAGTTGCTATTA+TGG 0.198733 4_4:-34056881 Msa0678380:three_prime_UTR
GGCAAAAGTGACCCATATTT+AGG 0.206565 4_4:-34056962 Msa0678380:three_prime_UTR
ATAGAAAACACAAATATTTC+AGG 0.228751 4_4:+34060016 None:intergenic
AGAGCATGTTTCCCTAAATA+TGG 0.237050 4_4:+34056950 None:intergenic
GATGTTGCAGCTGGTATTCT+TGG 0.255223 4_4:-34059940 Msa0678380:CDS
GATCATATTTCCCTTATGTT+AGG 0.278447 4_4:+34056919 None:intergenic
GTTGGGTGCAGCAGTGCTAT+TGG 0.296588 4_4:-34059703 Msa0678380:CDS
TTGGGTGCAGCAGTGCTATT+GGG 0.315421 4_4:-34059702 Msa0678380:CDS
CAATTGTGTTTGTTCTCAAC+AGG 0.330421 4_4:-34059643 Msa0678380:CDS
ATTGCAACCACCTAACAAAT+TGG 0.332978 4_4:+34059662 None:intergenic
CCTATTACCAGGTTAAAATA+AGG 0.345231 4_4:+34059577 None:intergenic
AGTCATGATGCCTTCATGTT+GGG 0.345983 4_4:-34059726 Msa0678380:CDS
GACGCCACGTGTCCCAAAAC+AGG 0.355530 4_4:+34056856 None:intergenic
GGATTGTCCATGCTGGTGAT+AGG 0.356876 4_4:-34057348 Msa0678380:CDS
GCAAAAGTGACCCATATTTA+GGG 0.373498 4_4:-34056961 Msa0678380:three_prime_UTR
CATTGTTACCACTGCTTCCA+TGG 0.385326 4_4:-34057214 Msa0678380:CDS
ATCATATTTCCCTTATGTTA+GGG 0.389772 4_4:+34056920 None:intergenic
CACCATCACTTTCTGTCCAT+TGG 0.397413 4_4:-34057282 Msa0678380:CDS
GATGCCTTCATGTTGGGGTT+GGG 0.408304 4_4:-34059720 Msa0678380:CDS
AAAGTACTGAGAGTTGTCTA+TGG 0.408478 4_4:+34059601 None:intergenic
ATCAGGGTTGTAGTGGCTGT+TGG 0.413714 4_4:-34057369 Msa0678380:intron
AGCTGGTATTCTTGGCTTTG+AGG 0.413890 4_4:-34059932 Msa0678380:CDS
TTGTCTCTTAATTGTTATCA+TGG 0.414420 4_4:-34059962 Msa0678380:CDS
TGATGCCTTCATGTTGGGGT+TGG 0.426974 4_4:-34059721 Msa0678380:CDS
GATTGTCCATGCTGGTGATA+GGG 0.456865 4_4:-34057347 Msa0678380:CDS
ATCACTTTCTGTCCATTGGT+GGG 0.474245 4_4:-34057278 Msa0678380:CDS
AAAACAGGTTAAGCATTTGA+AGG 0.491275 4_4:-34059775 Msa0678380:intron
CATCACTTTCTGTCCATTGG+TGG 0.493082 4_4:-34057279 Msa0678380:CDS
GAGCATGTTTCCCTAAATAT+GGG 0.497346 4_4:+34056951 None:intergenic
AGCAACTTGTTGAGAAGATA+AGG 0.497476 4_4:+34056888 None:intergenic
TAGAAAACACAAATATTTCA+GGG 0.504564 4_4:+34060017 None:intergenic
TACAAAATGGCTAGAGTAGG+AGG 0.525528 4_4:-34059994 Msa0678380:exon
TAGGGGCTATGTCAAACAAC+AGG 0.531371 4_4:-34057329 Msa0678380:CDS
AACATGAAGGCATCATGACT+TGG 0.534290 4_4:+34059729 None:intergenic
GTCATGATGCCTTCATGTTG+GGG 0.535583 4_4:-34059725 Msa0678380:CDS
GGCTGTTGGATTGTCCATGC+TGG 0.538448 4_4:-34057355 Msa0678380:CDS
TTCTCAACAGGAATTTGAGA+AGG 0.542952 4_4:-34059631 Msa0678380:CDS
CATATTTCCCTTATGTTAGG+GGG 0.548916 4_4:+34056922 None:intergenic
TCTTACAAAATACTAATCCA+TGG 0.550482 4_4:+34057197 None:intergenic
AGGTTAAGCATTTGAAGGTG+TGG 0.557434 4_4:-34059770 Msa0678380:CDS
CTATACAAAATGGCTAGAGT+AGG 0.569931 4_4:-34059997 Msa0678380:exon
GTTGTTGATCAGGGTTGTAG+TGG 0.569996 4_4:-34057376 Msa0678380:intron
AAGTCATGATGCCTTCATGT+TGG 0.571287 4_4:-34059727 Msa0678380:CDS
GTTATCATGGATGTTGCAGC+TGG 0.584347 4_4:-34059949 Msa0678380:CDS
AAGTACTGAGAGTTGTCTAT+GGG 0.588754 4_4:+34059602 None:intergenic
AAAACATGAAATCCCACCAA+TGG 0.590722 4_4:+34057266 None:intergenic
ATACTAATCCATGGAAGCAG+TGG 0.591196 4_4:+34057206 None:intergenic
ATTGTCCATGCTGGTGATAG+GGG 0.591559 4_4:-34057346 Msa0678380:CDS
TATGTCAAACAACAGGTCAA+AGG 0.592422 4_4:-34057322 Msa0678380:CDS
ACGCCACGTGTCCCAAAACA+GGG 0.593408 4_4:+34056857 None:intergenic
TGCACCCAACCCCAACATGA+AGG 0.603813 4_4:+34059716 None:intergenic
TACTGAGAGTTGTCTATGGG+AGG 0.604411 4_4:+34059605 None:intergenic
TCATATTTCCCTTATGTTAG+GGG 0.608027 4_4:+34056921 None:intergenic
AACAGGTCAAAGGATTCTTG+TGG 0.609881 4_4:-34057312 Msa0678380:CDS
GGCTGAAATAGCACAAAACA+AGG 0.619758 4_4:-34059911 Msa0678380:intron
AGTTCTAAGATTAGAAACAG+TGG 0.646953 4_4:+34060078 None:intergenic
CATAGCCCCTATCACCAGCA+TGG 0.647824 4_4:+34057341 None:intergenic
CACCAATGGACAGAAAGTGA+TGG 0.668068 4_4:+34057280 None:intergenic
CGCCACGTGTCCCAAAACAG+GGG 0.699563 4_4:+34056858 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTGAAATAATTAATAACA+AGG - chr4_4:34057102-34057121 Msa0678380:three_prime_UTR 10.0%
!!! AAATAGTACTTTTTTTAATT+GGG - chr4_4:34059416-34059435 Msa0678380:intron 10.0%
!!! TTTTTTATTTTCAACAAAAC+AGG - chr4_4:34057150-34057169 Msa0678380:three_prime_UTR 15.0%
!!! TCCAAATAAAATATTTTACA+GGG + chr4_4:34058532-34058551 None:intergenic 15.0%
!!! ATCCAAATAAAATATTTTAC+AGG + chr4_4:34058533-34058552 None:intergenic 15.0%
!! TTGCAATTAAGAAAAAAAAA+AGG + chr4_4:34058851-34058870 None:intergenic 15.0%
!! AAAATGTTTGAATATCTAAT+CGG - chr4_4:34059037-34059056 Msa0678380:intron 15.0%
!!! CAAATAGTACTTTTTTTAAT+TGG - chr4_4:34059415-34059434 Msa0678380:intron 15.0%
!!! AATAGTACTTTTTTTAATTG+GGG - chr4_4:34059417-34059436 Msa0678380:intron 15.0%
!! ATATTTAGTGAATGATTATT+TGG + chr4_4:34059524-34059543 None:intergenic 15.0%
!!! TTTTGCCTTTTTTTTAATAA+GGG + chr4_4:34059965-34059984 None:intergenic 15.0%
!! TAGAAAACACAAATATTTCA+GGG + chr4_4:34056926-34056945 None:intergenic 20.0%
!! ATAGAAAACACAAATATTTC+AGG + chr4_4:34056927-34056946 None:intergenic 20.0%
!!! TTTGTGTTTTCTATACAAAA+TGG - chr4_4:34056933-34056952 Msa0678380:three_prime_UTR 20.0%
!!! TGTTTTTTTGAATCAAATGT+TGG - chr4_4:34058048-34058067 Msa0678380:intron 20.0%
!!! TCAATGTTTTGAAGTTTATT+TGG - chr4_4:34058087-34058106 Msa0678380:intron 20.0%
!! AAAAAAATCACAAAACATCA+TGG + chr4_4:34058204-34058223 None:intergenic 20.0%
!!! CCAAATAAAATATTTTACAG+GGG + chr4_4:34058531-34058550 None:intergenic 20.0%
!! CAAATAATCTTCAAATTACA+AGG + chr4_4:34058561-34058580 None:intergenic 20.0%
!! AAAATTTGCACAAATTAACA+GGG + chr4_4:34058673-34058692 None:intergenic 20.0%
!! ATTGTACAAAATCAATGATA+TGG + chr4_4:34058708-34058727 None:intergenic 20.0%
!!! ATCCAAAAAGAAAATTTTAC+AGG - chr4_4:34058778-34058797 Msa0678380:intron 20.0%
!!! TCCAAAAAGAAAATTTTACA+GGG - chr4_4:34058779-34058798 Msa0678380:intron 20.0%
!! AAATGTTTGAATATCTAATC+GGG - chr4_4:34059038-34059057 Msa0678380:intron 20.0%
!!! ATTTTAAAGAAAAGTGAAAG+TGG - chr4_4:34059113-34059132 Msa0678380:intron 20.0%
!!! ACAATTAGGTCATTTTAATT+CGG - chr4_4:34059232-34059251 Msa0678380:intron 20.0%
!! TATTGGATCAAAATACATTT+AGG + chr4_4:34059464-34059483 None:intergenic 20.0%
!!! CTTTTGCCTTTTTTTTAATA+AGG + chr4_4:34059966-34059985 None:intergenic 20.0%
! TTGTCTCTTAATTGTTATCA+TGG - chr4_4:34056978-34056997 Msa0678380:three_prime_UTR 25.0%
!!! CTTATTTTAACCTGGTAATA+GGG - chr4_4:34057364-34057383 Msa0678380:CDS 25.0%
!! CTATTCTATGAAGTATTAGT+CGG + chr4_4:34057562-34057581 None:intergenic 25.0%
!! ATAACCTGACATTTTTCATT+AGG + chr4_4:34057617-34057636 None:intergenic 25.0%
! AGAATAAAGTCATGAGAAAA+AGG + chr4_4:34057686-34057705 None:intergenic 25.0%
! AAAATAGAAAAGTGGTTGTT+GGG + chr4_4:34057733-34057752 None:intergenic 25.0%
!! AAAGTTGCATTTATTTCTCT+GGG + chr4_4:34057889-34057908 None:intergenic 25.0%
!! AAAAGTTGCATTTATTTCTC+TGG + chr4_4:34057890-34057909 None:intergenic 25.0%
!! TTTTGTATGAATATTGCTAC+TGG - chr4_4:34058117-34058136 Msa0678380:intron 25.0%
! TTGGAATAACAAAACTAGTT+AGG - chr4_4:34058430-34058449 Msa0678380:intron 25.0%
!! CCCCTGTAAAATATTTTATT+TGG - chr4_4:34058528-34058547 Msa0678380:intron 25.0%
! GAAATTTCCTAAAATTACAG+GGG + chr4_4:34058611-34058630 None:intergenic 25.0%
! CGAAATTTCCTAAAATTACA+GGG + chr4_4:34058612-34058631 None:intergenic 25.0%
!!! CCTGTAATTTTAGGAAATTT+CGG - chr4_4:34058610-34058629 Msa0678380:intron 25.0%
! AAATTTGCACAAATTAACAG+GGG + chr4_4:34058672-34058691 None:intergenic 25.0%
! CAAAATTTGCACAAATTAAC+AGG + chr4_4:34058674-34058693 None:intergenic 25.0%
!!! CTGTTAATTTGTGCAAATTT+TGG - chr4_4:34058672-34058691 Msa0678380:intron 25.0%
!!! AATTTGAAGTCTTTTGGTTT+TGG + chr4_4:34058747-34058766 None:intergenic 25.0%
!! CCAAAAAGAAAATTTTACAG+GGG - chr4_4:34058780-34058799 Msa0678380:intron 25.0%
! TGGAAATGACATATATTACA+GGG - chr4_4:34058809-34058828 Msa0678380:intron 25.0%
! TTGTATCATTGTGATACTTA+TGG + chr4_4:34058880-34058899 None:intergenic 25.0%
! GTATCACAATGATACAATTT+AGG - chr4_4:34058883-34058902 Msa0678380:intron 25.0%
! ACGTAAATTTGGAGAAAATT+GGG + chr4_4:34058933-34058952 None:intergenic 25.0%
! AACGTAAATTTGGAGAAAAT+TGG + chr4_4:34058934-34058953 None:intergenic 25.0%
! AATGTTTGAATATCTAATCG+GGG - chr4_4:34059039-34059058 Msa0678380:intron 25.0%
!!! CAATTAGGTCATTTTAATTC+GGG - chr4_4:34059233-34059252 Msa0678380:intron 25.0%
! TATGTTTCCGAATAAAACTA+TGG + chr4_4:34059369-34059388 None:intergenic 25.0%
! TGTCAGTTACATTTAGTTAT+TGG + chr4_4:34059481-34059500 None:intergenic 25.0%
! TCTTACAAAATACTAATCCA+TGG + chr4_4:34059746-34059765 None:intergenic 25.0%
!! CTTTTGTAATAATTGTGCTA+AGG - chr4_4:34059856-34059875 Msa0678380:intron 25.0%
! TTGGTCCCTTATTAAAAAAA+AGG - chr4_4:34059957-34059976 Msa0678380:CDS 25.0%
! ATCATATTTCCCTTATGTTA+GGG + chr4_4:34060023-34060042 None:intergenic 25.0%
!! AGTTCTAAGATTAGAAACAG+TGG + chr4_4:34056865-34056884 None:intergenic 30.0%
AAAACAGGTTAAGCATTTGA+AGG - chr4_4:34057165-34057184 Msa0678380:three_prime_UTR 30.0%
!!! CATGTTTTAGCCAATTTGTT+AGG - chr4_4:34057268-34057287 Msa0678380:CDS 30.0%
!!! TACTTTGCCTTATTTTAACC+TGG - chr4_4:34057356-34057375 Msa0678380:CDS 30.0%
CCTATTACCAGGTTAAAATA+AGG + chr4_4:34057366-34057385 None:intergenic 30.0%
!!! CCTTATTTTAACCTGGTAAT+AGG - chr4_4:34057363-34057382 Msa0678380:CDS 30.0%
!! GTAATTCGGATTAGCAAAAA+CGG - chr4_4:34057471-34057490 Msa0678380:intron 30.0%
! CTGACATTTTTCATTAGGAT+GGG + chr4_4:34057612-34057631 None:intergenic 30.0%
!! AAAAAGGAGTTTTAGCATGT+GGG + chr4_4:34057670-34057689 None:intergenic 30.0%
GAAAATAGAAAAGTGGTTGT+TGG + chr4_4:34057734-34057753 None:intergenic 30.0%
GAAGAGAGAAAATAGAAAAG+TGG + chr4_4:34057741-34057760 None:intergenic 30.0%
ATCTGTTCAATTAAAGCTTG+TGG - chr4_4:34057917-34057936 Msa0678380:intron 30.0%
! GAATCAAATGTTGGTTGTAT+AGG - chr4_4:34058057-34058076 Msa0678380:intron 30.0%
TGGATGATAAGCTTAATCAA+TGG - chr4_4:34058137-34058156 Msa0678380:intron 30.0%
! TTGTTATTCCAAAACAAGAC+AGG + chr4_4:34058422-34058441 None:intergenic 30.0%
! ATATTTTACAGGGGAAAAAC+CGG + chr4_4:34058522-34058541 None:intergenic 30.0%
! ATTTTGTCCCCTGTAATTTT+AGG - chr4_4:34058601-34058620 Msa0678380:intron 30.0%
CCGAAATTTCCTAAAATTAC+AGG + chr4_4:34058613-34058632 None:intergenic 30.0%
! TGAAATTTGCTCAAATTGCA+GGG + chr4_4:34058643-34058662 None:intergenic 30.0%
AATTTGCACAAATTAACAGG+GGG + chr4_4:34058671-34058690 None:intergenic 30.0%
ACAAAATCAATGATATGGCA+GGG + chr4_4:34058703-34058722 None:intergenic 30.0%
TACAAAATCAATGATATGGC+AGG + chr4_4:34058704-34058723 None:intergenic 30.0%
!! CCTTGTAATTTGAAGTCTTT+TGG + chr4_4:34058753-34058772 None:intergenic 30.0%
CCAAAAGACTTCAAATTACA+AGG - chr4_4:34058750-34058769 Msa0678380:intron 30.0%
AAGACTTCAAATTACAAGGA+CGG - chr4_4:34058754-34058773 Msa0678380:intron 30.0%
! CCCCTGTAAAATTTTCTTTT+TGG + chr4_4:34058783-34058802 None:intergenic 30.0%
! AAATTTTACAGGGGAAAAAC+TGG - chr4_4:34058789-34058808 Msa0678380:intron 30.0%
CTGGAAATGACATATATTAC+AGG - chr4_4:34058808-34058827 Msa0678380:intron 30.0%
GGAAATGACATATATTACAG+GGG - chr4_4:34058810-34058829 Msa0678380:intron 30.0%
GAAATGACATATATTACAGG+GGG - chr4_4:34058811-34058830 Msa0678380:intron 30.0%
AATTTGGAGAAAATTGGGAT+GGG + chr4_4:34058928-34058947 None:intergenic 30.0%
AAATTTGGAGAAAATTGGGA+TGG + chr4_4:34058929-34058948 None:intergenic 30.0%
ATGTCATCTCAACGTAAATT+TGG + chr4_4:34058944-34058963 None:intergenic 30.0%
GTATTAAAGAATAGCCACAT+TGG + chr4_4:34058998-34059017 None:intergenic 30.0%
! TGTCACTTCAGTGAAAAATT+TGG - chr4_4:34059168-34059187 Msa0678380:intron 30.0%
ATTGCACAATCATGATACTT+AGG - chr4_4:34059200-34059219 Msa0678380:intron 30.0%
TTGCACAATCATGATACTTA+GGG - chr4_4:34059201-34059220 Msa0678380:intron 30.0%
! TTAGGGTCAAAAGTACAATT+AGG - chr4_4:34059218-34059237 Msa0678380:intron 30.0%
!!! CTACGTACCATAGTTTTATT+CGG - chr4_4:34059359-34059378 Msa0678380:intron 30.0%
GATCAACAACACAATCAAAA+GGG + chr4_4:34059554-34059573 None:intergenic 30.0%
TGATCAACAACACAATCAAA+AGG + chr4_4:34059555-34059574 None:intergenic 30.0%
!! TTTTGATTGTGTTGTTGATC+AGG - chr4_4:34059554-34059573 Msa0678380:intron 30.0%
! TTTGATTGTGTTGTTGATCA+GGG - chr4_4:34059555-34059574 Msa0678380:intron 30.0%
!!! GTTCATGTTTTTAGTGCTTT+TGG - chr4_4:34059788-34059807 Msa0678380:intron 30.0%
!!! GCAACATTTTGAAACTTATG+GGG + chr4_4:34059933-34059952 None:intergenic 30.0%
!!! CGCAACATTTTGAAACTTAT+GGG + chr4_4:34059934-34059953 None:intergenic 30.0%
!!! TCGCAACATTTTGAAACTTA+TGG + chr4_4:34059935-34059954 None:intergenic 30.0%
! GTTTCAAAATGTTGCGATTT+TGG - chr4_4:34059938-34059957 Msa0678380:CDS 30.0%
TCATATTTCCCTTATGTTAG+GGG + chr4_4:34060022-34060041 None:intergenic 30.0%
GATCATATTTCCCTTATGTT+AGG + chr4_4:34060024-34060043 None:intergenic 30.0%
! TTCTCAACAAGTTGCTATTA+TGG - chr4_4:34060059-34060078 Msa0678380:five_prime_UTR 30.0%
! GATTTTCTTTCTGTTCTAGC+TGG + chr4_4:34056904-34056923 None:intergenic 35.0%
CTATACAAAATGGCTAGAGT+AGG - chr4_4:34056943-34056962 Msa0678380:three_prime_UTR 35.0%
!!! GTTTTAGCCAATTTGTTAGG+TGG - chr4_4:34057271-34057290 Msa0678380:CDS 35.0%
ATTGCAACCACCTAACAAAT+TGG + chr4_4:34057281-34057300 None:intergenic 35.0%
CAATTGTGTTTGTTCTCAAC+AGG - chr4_4:34057297-34057316 Msa0678380:CDS 35.0%
TTCTCAACAGGAATTTGAGA+AGG - chr4_4:34057309-34057328 Msa0678380:CDS 35.0%
!! AAGTACTGAGAGTTGTCTAT+GGG + chr4_4:34057341-34057360 None:intergenic 35.0%
!! AAAGTACTGAGAGTTGTCTA+TGG + chr4_4:34057342-34057361 None:intergenic 35.0%
! TCTATGAAGTATTAGTCGGT+CGG + chr4_4:34057558-34057577 None:intergenic 35.0%
! CCTGACATTTTTCATTAGGA+TGG + chr4_4:34057613-34057632 None:intergenic 35.0%
CCATCCTAATGAAAAATGTC+AGG - chr4_4:34057610-34057629 Msa0678380:intron 35.0%
!! AAAAGGAGTTTTAGCATGTG+GGG + chr4_4:34057669-34057688 None:intergenic 35.0%
!! GAAAAAGGAGTTTTAGCATG+TGG + chr4_4:34057671-34057690 None:intergenic 35.0%
AGAAGAAAAGGAAGAACTGT+GGG + chr4_4:34057824-34057843 None:intergenic 35.0%
GATAGAGTTTGGAGAAGAAA+AGG + chr4_4:34057836-34057855 None:intergenic 35.0%
GGTATGCATATGATAGAGTT+TGG + chr4_4:34057847-34057866 None:intergenic 35.0%
GGTTGAAGCAACAAAAGAAT+GGG + chr4_4:34057868-34057887 None:intergenic 35.0%
ACTCATGTCAAATCTACTTG+TGG + chr4_4:34057991-34058010 None:intergenic 35.0%
AGAGATATATTACCAGGTAG+CGG + chr4_4:34058393-34058412 None:intergenic 35.0%
! GTGTTTTACCCCTGTAATAA+AGG - chr4_4:34058490-34058509 Msa0678380:intron 35.0%
GGAAATGACCTTTATTACAG+GGG + chr4_4:34058501-34058520 None:intergenic 35.0%
CGGAAATGACCTTTATTACA+GGG + chr4_4:34058502-34058521 None:intergenic 35.0%
CCTGTAATAAAGGTCATTTC+CGG - chr4_4:34058500-34058519 Msa0678380:intron 35.0%
! GGGGACAAAATTTTTCATGA+AGG + chr4_4:34058592-34058611 None:intergenic 35.0%
! GTGAAATTTGCTCAAATTGC+AGG + chr4_4:34058644-34058663 None:intergenic 35.0%
CAAAATCAATGATATGGCAG+GGG + chr4_4:34058702-34058721 None:intergenic 35.0%
ATGGGGACTATAAAGCATAA+GGG + chr4_4:34058910-34058929 None:intergenic 35.0%
ATTTGGAGAAAATTGGGATG+GGG + chr4_4:34058927-34058946 None:intergenic 35.0%
CACTTCAGTGAAAAATTTGG+AGG - chr4_4:34059171-34059190 Msa0678380:intron 35.0%
ACTATGGTACGTAGTCTAAT+AGG + chr4_4:34059353-34059372 None:intergenic 35.0%
TATGTCAAACAACAGGTCAA+AGG - chr4_4:34059618-34059637 Msa0678380:CDS 35.0%
AAAACATGAAATCCCACCAA+TGG + chr4_4:34059677-34059696 None:intergenic 35.0%
!! GGGATTTCATGTTTTGTTCA+TGG - chr4_4:34059682-34059701 Msa0678380:CDS 35.0%
GCAAAAGTGACCCATATTTA+GGG - chr4_4:34059979-34059998 Msa0678380:CDS 35.0%
GAGCATGTTTCCCTAAATAT+GGG + chr4_4:34059992-34060011 None:intergenic 35.0%
AGAGCATGTTTCCCTAAATA+TGG + chr4_4:34059993-34060012 None:intergenic 35.0%
CATATTTCCCTTATGTTAGG+GGG + chr4_4:34060021-34060040 None:intergenic 35.0%
!! AGCAACTTGTTGAGAAGATA+AGG + chr4_4:34060055-34060074 None:intergenic 35.0%
TACAAAATGGCTAGAGTAGG+AGG - chr4_4:34056946-34056965 Msa0678380:three_prime_UTR 40.0%
GGCTGAAATAGCACAAAACA+AGG - chr4_4:34057029-34057048 Msa0678380:three_prime_UTR 40.0%
AGGTTAAGCATTTGAAGGTG+TGG - chr4_4:34057170-34057189 Msa0678380:three_prime_UTR 40.0%
!! AACATGAAGGCATCATGACT+TGG + chr4_4:34057214-34057233 None:intergenic 40.0%
AAGTCATGATGCCTTCATGT+TGG - chr4_4:34057213-34057232 Msa0678380:CDS 40.0%
AGTCATGATGCCTTCATGTT+GGG - chr4_4:34057214-34057233 Msa0678380:CDS 40.0%
! TTTTAACCTGGTAATAGGGC+TGG - chr4_4:34057368-34057387 Msa0678380:intron 40.0%
! TTAGCAAAAACGGACTTGGA+TGG - chr4_4:34057481-34057500 Msa0678380:intron 40.0%
! TAGCAAAAACGGACTTGGAT+GGG - chr4_4:34057482-34057501 Msa0678380:intron 40.0%
AAGTATTAGTCGGTCGGATA+TGG + chr4_4:34057552-34057571 None:intergenic 40.0%
!! AAAGGAGTTTTAGCATGTGG+GGG + chr4_4:34057668-34057687 None:intergenic 40.0%
GGAAGAGAGAAGAAAGAGAA+GGG + chr4_4:34057776-34057795 None:intergenic 40.0%
TGGAAGAGAGAAGAAAGAGA+AGG + chr4_4:34057777-34057796 None:intergenic 40.0%
AAGGAAAACAGGAAGGAGTT+TGG + chr4_4:34057797-34057816 None:intergenic 40.0%
GAAGAAAAGGAAGAACTGTG+GGG + chr4_4:34057823-34057842 None:intergenic 40.0%
GAGAAGAAAAGGAAGAACTG+TGG + chr4_4:34057825-34057844 None:intergenic 40.0%
GGGTTGAAGCAACAAAAGAA+TGG + chr4_4:34057869-34057888 None:intergenic 40.0%
AGAGGCAGAGATATATTACC+AGG + chr4_4:34058399-34058418 None:intergenic 40.0%
ATTCCAAAACAAGACAGGAG+AGG + chr4_4:34058417-34058436 None:intergenic 40.0%
CCGGAAATGACCTTTATTAC+AGG + chr4_4:34058503-34058522 None:intergenic 40.0%
! AATTTGCTCAAATTGCAGGG+AGG + chr4_4:34058640-34058659 None:intergenic 40.0%
TGGGGACTATAAAGCATAAG+GGG + chr4_4:34058909-34058928 None:intergenic 40.0%
GATGGGGACTATAAAGCATA+AGG + chr4_4:34058911-34058930 None:intergenic 40.0%
AACAGGTCAAAGGATTCTTG+TGG - chr4_4:34059628-34059647 Msa0678380:CDS 40.0%
ATCACTTTCTGTCCATTGGT+GGG - chr4_4:34059662-34059681 Msa0678380:CDS 40.0%
ATACTAATCCATGGAAGCAG+TGG + chr4_4:34059737-34059756 None:intergenic 40.0%
GGCAAAAGTGACCCATATTT+AGG - chr4_4:34059978-34059997 Msa0678380:CDS 40.0%
! TCTTTTGACCCCCTAACATA+AGG - chr4_4:34060010-34060029 Msa0678380:exon 40.0%
! CTTTTGACCCCCTAACATAA+GGG - chr4_4:34060011-34060030 Msa0678380:five_prime_UTR 40.0%
GTTATCATGGATGTTGCAGC+TGG - chr4_4:34056991-34057010 Msa0678380:three_prime_UTR 45.0%
GATGTTGCAGCTGGTATTCT+TGG - chr4_4:34057000-34057019 Msa0678380:three_prime_UTR 45.0%
!! AGCTGGTATTCTTGGCTTTG+AGG - chr4_4:34057008-34057027 Msa0678380:three_prime_UTR 45.0%
GTCATGATGCCTTCATGTTG+GGG - chr4_4:34057215-34057234 Msa0678380:CDS 45.0%
TACTGAGAGTTGTCTATGGG+AGG + chr4_4:34057338-34057357 None:intergenic 45.0%
!! CGGATTAGCAAAAACGGACT+TGG - chr4_4:34057477-34057496 Msa0678380:intron 45.0%
GGACTATATTCTACCAACCG+CGG - chr4_4:34058311-34058330 Msa0678380:intron 45.0%
! TACCTTTCGTTTGGGGTTCT+CGG - chr4_4:34058335-34058354 Msa0678380:intron 45.0%
! GATATATTACCAGGTAGCGG+TGG + chr4_4:34058390-34058409 None:intergenic 45.0%
GGTGTCAGCTTAGTAGAGTT+TGG + chr4_4:34059332-34059351 None:intergenic 45.0%
GTTGTTGATCAGGGTTGTAG+TGG - chr4_4:34059564-34059583 Msa0678380:intron 45.0%
GATTGTCCATGCTGGTGATA+GGG - chr4_4:34059593-34059612 Msa0678380:CDS 45.0%
ATTGTCCATGCTGGTGATAG+GGG - chr4_4:34059594-34059613 Msa0678380:CDS 45.0%
TAGGGGCTATGTCAAACAAC+AGG - chr4_4:34059611-34059630 Msa0678380:CDS 45.0%
CACCATCACTTTCTGTCCAT+TGG - chr4_4:34059658-34059677 Msa0678380:CDS 45.0%
CACCAATGGACAGAAAGTGA+TGG + chr4_4:34059663-34059682 None:intergenic 45.0%
CATCACTTTCTGTCCATTGG+TGG - chr4_4:34059661-34059680 Msa0678380:CDS 45.0%
CATTGTTACCACTGCTTCCA+TGG - chr4_4:34059726-34059745 Msa0678380:CDS 45.0%
! TGCTATTATGGCCCCTGTTT+TGG - chr4_4:34060071-34060090 Msa0678380:five_prime_UTR 45.0%
! GCTATTATGGCCCCTGTTTT+GGG - chr4_4:34060072-34060091 Msa0678380:five_prime_UTR 45.0%
TGATGCCTTCATGTTGGGGT+TGG - chr4_4:34057219-34057238 Msa0678380:CDS 50.0%
GATGCCTTCATGTTGGGGTT+GGG - chr4_4:34057220-34057239 Msa0678380:CDS 50.0%
! TTGGGTGCAGCAGTGCTATT+GGG - chr4_4:34057238-34057257 Msa0678380:CDS 50.0%
TGTTGTCCAGCCCTATTACC+AGG + chr4_4:34057377-34057396 None:intergenic 50.0%
TCATTCGAAACGGGCGGTTT+GGG + chr4_4:34057413-34057432 None:intergenic 50.0%
TGCACACGGGTCATTCGAAA+CGG + chr4_4:34057423-34057442 None:intergenic 50.0%
! GGTCGGATATGGATTGTCGT+GGG + chr4_4:34057541-34057560 None:intergenic 50.0%
GGGGAAGAAGGAAAACAGGA+AGG + chr4_4:34057804-34057823 None:intergenic 50.0%
CTGTGGGGAAGAAGGAAAAC+AGG + chr4_4:34057808-34057827 None:intergenic 50.0%
AGGAAGAACTGTGGGGAAGA+AGG + chr4_4:34057816-34057835 None:intergenic 50.0%
TCGTCGGAATGCGGTTTATG+CGG + chr4_4:34058249-34058268 None:intergenic 50.0%
AAACGAAAGGTAACCGCGGT+TGG + chr4_4:34058327-34058346 None:intergenic 50.0%
AACCGCGGTTACCTTTCGTT+TGG - chr4_4:34058326-34058345 Msa0678380:intron 50.0%
ACCGCGGTTACCTTTCGTTT+GGG - chr4_4:34058327-34058346 Msa0678380:intron 50.0%
!!! CTGCCTCTCCTGTCTTGTTT+TGG - chr4_4:34058411-34058430 Msa0678380:intron 50.0%
CCACATTGGCCAAGAGAGTT+GGG + chr4_4:34058984-34059003 None:intergenic 50.0%
ATCAGGGTTGTAGTGGCTGT+TGG - chr4_4:34059571-34059590 Msa0678380:intron 50.0%
GGATTGTCCATGCTGGTGAT+AGG - chr4_4:34059592-34059611 Msa0678380:CDS 50.0%
TGCACCCAACCCCAACATGA+AGG + chr4_4:34057227-34057246 None:intergenic 55.0%
GTTGGGTGCAGCAGTGCTAT+TGG - chr4_4:34057237-34057256 Msa0678380:CDS 55.0%
GTCATTCGAAACGGGCGGTT+TGG + chr4_4:34057414-34057433 None:intergenic 55.0%
GCACACGGGTCATTCGAAAC+GGG + chr4_4:34057422-34057441 None:intergenic 55.0%
TCGAATGACCCGTGTGCAAG+CGG - chr4_4:34057425-34057444 Msa0678380:intron 55.0%
TAAACCGACCCGCTTGCACA+CGG + chr4_4:34057437-34057456 None:intergenic 55.0%
CGGACTTGGATGGGTGCTAA+CGG - chr4_4:34057491-34057510 Msa0678380:intron 55.0%
CTTGGATGGGTGCTAACGGT+CGG - chr4_4:34057495-34057514 Msa0678380:intron 55.0%
! CGGTCGGATATGGATTGTCG+TGG + chr4_4:34057542-34057561 None:intergenic 55.0%
TTTATGCGGTCGGTGGACAC+GGG + chr4_4:34058235-34058254 None:intergenic 55.0%
GTTTATGCGGTCGGTGGACA+CGG + chr4_4:34058236-34058255 None:intergenic 55.0%
AATGCGGTTTATGCGGTCGG+TGG + chr4_4:34058242-34058261 None:intergenic 55.0%
CGGAATGCGGTTTATGCGGT+CGG + chr4_4:34058245-34058264 None:intergenic 55.0%
!! TTGACGGGTTCGTCGGAATG+CGG + chr4_4:34058258-34058277 None:intergenic 55.0%
TCCATGCGAACGCTCGGTTA+CGG + chr4_4:34058284-34058303 None:intergenic 55.0%
TAACCGAGCGTTCGCATGGA+CGG - chr4_4:34058284-34058303 Msa0678380:intron 55.0%
TTTCCGTCCATGCGAACGCT+CGG + chr4_4:34058290-34058309 None:intergenic 55.0%
AGCGTTCGCATGGACGGAAA+CGG - chr4_4:34058290-34058309 Msa0678380:intron 55.0%
CCCCAAACGAAAGGTAACCG+CGG + chr4_4:34058331-34058350 None:intergenic 55.0%
CCGCGGTTACCTTTCGTTTG+GGG - chr4_4:34058328-34058347 Msa0678380:intron 55.0%
GTCCGAGAACCCCAAACGAA+AGG + chr4_4:34058340-34058359 None:intergenic 55.0%
! TACCAGGTAGCGGTGGACAA+CGG + chr4_4:34058383-34058402 None:intergenic 55.0%
GCCAAGAGAGTTGGGAGTCA+CGG + chr4_4:34058976-34058995 None:intergenic 55.0%
CCCAACTCTCTTGGCCAATG+TGG - chr4_4:34058981-34059000 Msa0678380:intron 55.0%
GCCACATTGGCCAAGAGAGT+TGG + chr4_4:34058985-34059004 None:intergenic 55.0%
GGCTGTTGGATTGTCCATGC+TGG - chr4_4:34059585-34059604 Msa0678380:CDS 55.0%
CATAGCCCCTATCACCAGCA+TGG + chr4_4:34059602-34059621 None:intergenic 55.0%
! TTCGAAACGGGCGGTTTGGG+CGG + chr4_4:34057410-34057429 None:intergenic 60.0%
CACGGGTCATTCGAAACGGG+CGG + chr4_4:34057419-34057438 None:intergenic 60.0%
CGAATGACCCGTGTGCAAGC+GGG - chr4_4:34057426-34057445 Msa0678380:intron 60.0%
AAACCGACCCGCTTGCACAC+GGG + chr4_4:34057436-34057455 None:intergenic 60.0%
! GTGTGCAAGCGGGTCGGTTT+AGG - chr4_4:34057436-34057455 Msa0678380:intron 60.0%
! TGCAAGCGGGTCGGTTTAGG+CGG - chr4_4:34057439-34057458 Msa0678380:intron 60.0%
TGGGTGCTAACGGTCGGTTG+CGG - chr4_4:34057501-34057520 Msa0678380:intron 60.0%
TGCTAACGGTCGGTTGCGGT+TGG - chr4_4:34057505-34057524 Msa0678380:intron 60.0%
GCTAACGGTCGGTTGCGGTT+GGG - chr4_4:34057506-34057525 Msa0678380:intron 60.0%
GTCGGTGGACACGGGTATGA+AGG + chr4_4:34058227-34058246 None:intergenic 60.0%
ATGCGAACGCTCGGTTACGG+CGG + chr4_4:34058281-34058300 None:intergenic 60.0%
! GGTCGGGTTCGGTCGGAATT+CGG + chr4_4:34058362-34058381 None:intergenic 60.0%
! AGGTAGCGGTGGACAACGGT+CGG + chr4_4:34058379-34058398 None:intergenic 60.0%
GCCGTGACTCCCAACTCTCT+TGG - chr4_4:34058972-34058991 Msa0678380:intron 60.0%
TGACCCGTGTGCAAGCGGGT+CGG - chr4_4:34057430-34057449 Msa0678380:intron 65.0%
! GCAAGCGGGTCGGTTTAGGC+GGG - chr4_4:34057440-34057459 Msa0678380:intron 65.0%
! GTCGGTTTAGGCGGGTCGCT+CGG - chr4_4:34057448-34057467 Msa0678380:intron 65.0%
TCGGTTTAGGCGGGTCGCTC+GGG - chr4_4:34057449-34057468 Msa0678380:intron 65.0%
GGCGGGTCGCTCGGGTAATT+CGG - chr4_4:34057457-34057476 Msa0678380:intron 65.0%
!! ACGGCGGTTGACGGGTTCGT+CGG + chr4_4:34058265-34058284 None:intergenic 65.0%
GCTCGGTTACGGCGGTTGAC+GGG + chr4_4:34058273-34058292 None:intergenic 65.0%
CGCTCGGTTACGGCGGTTGA+CGG + chr4_4:34058274-34058293 None:intergenic 65.0%
GCCGTAACCGAGCGTTCGCA+TGG - chr4_4:34058280-34058299 Msa0678380:intron 65.0%
! GGACAACGGTCGGGTTCGGT+CGG + chr4_4:34058369-34058388 None:intergenic 65.0%
CGGTGGACAACGGTCGGGTT+CGG + chr4_4:34058373-34058392 None:intergenic 65.0%
! GGTAGCGGTGGACAACGGTC+GGG + chr4_4:34058378-34058397 None:intergenic 65.0%
GACCGTTGTCCACCGCTACC+TGG - chr4_4:34058378-34058397 Msa0678380:intron 65.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 34056861 34060101 34056861 ID=Msa0678380;Name=Msa0678380
chr4_4 mRNA 34056861 34060101 34056861 ID=Msa0678380-mRNA-1;Parent=Msa0678380;Name=Msa0678380-mRNA-1;_AED=0.26;_eAED=0.26;_QI=91|1|1|1|1|1|3|348|160
chr4_4 exon 34056861 34057386 34056861 ID=Msa0678380-mRNA-1:exon:8205;Parent=Msa0678380-mRNA-1
chr4_4 exon 34059585 34059790 34059585 ID=Msa0678380-mRNA-1:exon:8204;Parent=Msa0678380-mRNA-1
chr4_4 exon 34059912 34060101 34059912 ID=Msa0678380-mRNA-1:exon:8203;Parent=Msa0678380-mRNA-1
chr4_4 five_prime_UTR 34060011 34060101 34060011 ID=Msa0678380-mRNA-1:five_prime_utr;Parent=Msa0678380-mRNA-1
chr4_4 CDS 34059912 34060010 34059912 ID=Msa0678380-mRNA-1:cds;Parent=Msa0678380-mRNA-1
chr4_4 CDS 34059585 34059790 34059585 ID=Msa0678380-mRNA-1:cds;Parent=Msa0678380-mRNA-1
chr4_4 CDS 34057209 34057386 34057209 ID=Msa0678380-mRNA-1:cds;Parent=Msa0678380-mRNA-1
chr4_4 three_prime_UTR 34056861 34057208 34056861 ID=Msa0678380-mRNA-1:three_prime_utr;Parent=Msa0678380-mRNA-1
Gene Sequence

>Msa0678380

ATGGCTAGAGTAGGAGGCATTGTTGTTTGTCTCTTAATTGTTATCATGGATGTTGCAGCTGGTATTCTTGGCTTTGAGGCTGAAATAGCACAAAACAAGGTTAAGCATTTGAAGGTGTGGATATTTGAGTGTAGAGAACCAAGTCATGATGCCTTCATGTTGGGGTTGGGTGCAGCAGTGCTATTGGGACTATCACATGTTTTAGCCAATTTGTTAGGTGGTTGCAATTGTGTTTGTTCTCAACAGGAATTTGAGAAGGCTTCCTCCCATAGACAACTCTCAGTACTTTGCCTTATTTTAACCTGGGTTGTAGTGGCTGTTGGATTGTCCATGCTGGTGATAGGGGCTATGTCAAACAACAGGTCAAAGGATTCTTGTGGTTTCTCACACCATCACTTTCTGTCCATTGGTGGGATTTCATGTTTTGTTCATGGTCTCTTCTCTGTTATATATTACATTGTTACCACTGCTTCCATGGATTAG
Protein sequence

>Msa0678380

MARVGGIVVCLLIVIMDVAAGILGFEAEIAQNKVKHLKVWIFECREPSHDAFMLGLGAAVLLGLSHVLANLLGGCNCVCSQQEFEKASSHRQLSVLCLILTWVVVAVGLSMLVIGAMSNNRSKDSCGFSHHHFLSIGGISCFVHGLFSVIYYIVTTASMD*