AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1061930

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1061930 MtrunA17_Chr7g0240771 85.258 407 36 9 1 400 1 390 0.0 630
Msa1061930 MtrunA17_Chr4g0008811 29.428 367 172 17 51 400 9 305 8.07e-21 92.0
Msa1061930 MtrunA17_Chr5g0436151 30.867 392 189 19 44 400 18 362 4.26e-16 79.0
Msa1061930 MtrunA17_Chr1g0185611 37.008 127 60 4 280 400 80 192 1.63e-14 71.6
Msa1061930 MtrunA17_Chr2g0328681 39.535 129 50 6 279 400 214 321 1.93e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1061930 AT3G54000.1 30.714 420 192 20 5 400 8 352 1.12e-21 95.9
Msa1061930 AT3G54000.3 32.479 351 158 17 5 331 8 303 6.41e-21 93.2
Msa1061930 AT3G54000.2 30.606 330 151 16 5 311 8 282 1.26e-13 71.6

Find 107 sgRNAs with CRISPR-Local

Find 201 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAGAAAATGTGAAGGGATTT+TGG 0.181897 7_2:+52405750 None:intergenic
CTATATAGTCTTCAAGCTTT+AGG 0.234041 7_2:+52404099 None:intergenic
CTCTCCCATGTTTCGCATTT+CGG 0.242451 7_2:-52405837 Msa1061930:five_prime_UTR
CAATCAACCCTATGTGCTTT+TGG 0.244653 7_2:-52405240 Msa1061930:CDS
ACTGATATCACTGCAATTTC+AGG 0.266164 7_2:+52403408 None:intergenic
GTCTTCAAGCTTTAGGTTAA+GGG 0.268229 7_2:+52404106 None:intergenic
TGATGAACCTTTGCGGCATT+TGG 0.275294 7_2:+52405209 None:intergenic
CTGAAAGGCTGAACACAATA+AGG 0.279562 7_2:-52404850 Msa1061930:CDS
TCCACCTCTAATGTTGAAAA+TGG 0.300073 7_2:-52404038 Msa1061930:intron
CTGTAATACCACTGCAAATC+TGG 0.300724 7_2:+52403207 None:intergenic
GCGGGTATGCAGAAGCAAAA+TGG 0.310089 7_2:-52404827 Msa1061930:CDS
AGTCTTCAAGCTTTAGGTTA+AGG 0.313871 7_2:+52404105 None:intergenic
TGCTGCTGGCTGAGGCCTGC+TGG 0.319876 7_2:+52403758 None:intergenic
TAATAATAATAGAAGATAAA+TGG 0.327212 7_2:-52403510 Msa1061930:three_prime_UTR
AACAACAACGTTGCTGGTTT+AGG 0.336273 7_2:-52404740 Msa1061930:CDS
GCATTCTCACAAACACCATT+AGG 0.337817 7_2:+52405174 None:intergenic
ATTTGGATGACGGTGAGTTC+TGG 0.349881 7_2:-52405660 Msa1061930:CDS
TAGGTTTGTCATCATCTTCA+TGG 0.352410 7_2:-52404721 Msa1061930:CDS
GGAAATTGAAGTGAAAGAAA+AGG 0.359094 7_2:+52405725 None:intergenic
ATTTCATTGCTCACTGCTGC+TGG 0.359817 7_2:+52403744 None:intergenic
AGGGTTGAGGAGCTTGAAAT+TGG 0.368641 7_2:+52404672 None:intergenic
CGGGTATGCAGAAGCAAAAT+GGG 0.371790 7_2:-52404826 Msa1061930:CDS
TTGCTCACTGCTGCTGGCTG+AGG 0.373008 7_2:+52403750 None:intergenic
GTTATGATTCTCAGAGGCTT+TGG 0.379710 7_2:+52405390 None:intergenic
AAACACCATTAGGGCTTCCT+TGG 0.391799 7_2:+52405184 None:intergenic
TTGTTCATCATCGTCACTTT+CGG 0.392470 7_2:+52405465 None:intergenic
GTTGAATTTCTTGATTCAAC+AGG 0.398365 7_2:+52404581 None:intergenic
CATTCTCACAAACACCATTA+GGG 0.402675 7_2:+52405175 None:intergenic
GTTTCGCATTTCGGTAATCA+TGG 0.406161 7_2:-52405828 Msa1061930:five_prime_UTR
AGCGAAAGCGAAGAAGGTTT+CGG 0.413750 7_2:+52405583 None:intergenic
GAAATTGAAGTGAAAGAAAA+GGG 0.418507 7_2:+52405726 None:intergenic
AATAATAATAAATTGGTTTG+TGG 0.433439 7_2:-52403263 Msa1061930:three_prime_UTR
AATGGTTATGGTAGCAGTAC+TGG 0.435118 7_2:-52404626 Msa1061930:CDS
TCAAGCCAAGGAAGCCCTAA+TGG 0.437674 7_2:-52405189 Msa1061930:CDS
TTGTTGCACCTCCAAGGCAT+AGG 0.440174 7_2:-52403824 Msa1061930:CDS
CTTGGCTTGATGAACCTTTG+CGG 0.440983 7_2:+52405202 None:intergenic
AGGGGATTTGATGTAACAAA+AGG 0.449526 7_2:-52403617 Msa1061930:three_prime_UTR
GGGGAAGAAGAATTTCTTTG+AGG 0.452557 7_2:+52405072 None:intergenic
AAATAATGTTAGGAGCCTCT+TGG 0.453899 7_2:-52403464 Msa1061930:three_prime_UTR
AAAGCTTGAAGACTATATAG+TGG 0.470534 7_2:-52404096 Msa1061930:CDS
ACATTATTACCCCTATGCCT+TGG 0.482077 7_2:+52403813 None:intergenic
TTGAAAAGCGAAAGCGAAGA+AGG 0.482963 7_2:+52405577 None:intergenic
TATTCATAATAAGTAAACTA+AGG 0.483363 7_2:+52403158 None:intergenic
GGAGGAAGAAATGTTGTTGA+TGG 0.488016 7_2:-52404776 Msa1061930:CDS
TTATTACCCCTATGCCTTGG+AGG 0.489609 7_2:+52403816 None:intergenic
TGTTGAGCATGTTGTAGTGT+TGG 0.499882 7_2:+52404698 None:intergenic
TGTTGCACCTCCAAGGCATA+GGG 0.500195 7_2:-52403823 Msa1061930:CDS
GTCATTTCCAAAAGCACATA+GGG 0.503258 7_2:+52405233 None:intergenic
TCAGAACAAGCGAATCCATA+AGG 0.503685 7_2:+52405529 None:intergenic
ATGATGAAAATGATATGAAA+TGG 0.513671 7_2:-52403688 Msa1061930:three_prime_UTR
AAATGGTCCTTTGATTTGCT+TGG 0.515224 7_2:-52403671 Msa1061930:three_prime_UTR
TTTCAGCAAAGACAAACAAA+TGG 0.515645 7_2:-52404797 Msa1061930:CDS
TGTAGCTTGTGCAACTGCAT+TGG 0.518913 7_2:-52404150 Msa1061930:intron
TTTCGCATTTCGGTAATCAT+GGG 0.519762 7_2:-52405827 Msa1061930:five_prime_UTR
GATTACCGAAATGCGAAACA+TGG 0.528517 7_2:+52405832 None:intergenic
GTCATCCAAATTCTCAGCCA+TGG 0.531206 7_2:+52405672 None:intergenic
TGAAAGGCTGAACACAATAA+GGG 0.531358 7_2:-52404849 Msa1061930:CDS
ATAAGTAAACTAAGGTGGAC+AGG 0.532141 7_2:+52403166 None:intergenic
GAATCATAACAACAATAACT+TGG 0.540471 7_2:-52405377 Msa1061930:intron
AAGCTTGAAGACTATATAGT+GGG 0.550779 7_2:-52404095 Msa1061930:CDS
AGTCATTTCCAAAAGCACAT+AGG 0.552956 7_2:+52405232 None:intergenic
GGACAGGTTGGCATCAAAGA+AGG 0.554332 7_2:+52403182 None:intergenic
AGGAAAACAGCTCTCATGTA+GGG 0.557045 7_2:+52404653 None:intergenic
CAGAGGCTTTGGAATCAACA+TGG 0.562511 7_2:+52405401 None:intergenic
TTATTATGAATAATAATACA+AGG 0.563828 7_2:-52403147 None:intergenic
ACAGCTCTCATGTAGGGTTG+AGG 0.564250 7_2:+52404659 None:intergenic
TGCTACCATAACCATTTCCA+AGG 0.565265 7_2:+52404633 None:intergenic
TGCATTGGTTCCAGCTAGAG+TGG 0.566049 7_2:-52404135 Msa1061930:CDS
AAAAGGGAGAGAAAATGTGA+AGG 0.566760 7_2:+52405742 None:intergenic
AAGGAAAACAGCTCTCATGT+AGG 0.571063 7_2:+52404652 None:intergenic
TTTCTTGATTCAACAGGACG+AGG 0.572729 7_2:+52404587 None:intergenic
TAGCTGTTGTTGCACCTCCA+AGG 0.575430 7_2:-52403830 Msa1061930:CDS
AAAGGGAGAGAAAATGTGAA+GGG 0.576120 7_2:+52405743 None:intergenic
AAATCAAGCTTCCACAGGAG+TGG 0.577332 7_2:-52403725 Msa1061930:CDS
TGTGTCATCACCGTCGTCGT+CGG 0.577857 7_2:+52405618 None:intergenic
GCAGCAGCAACAATACCAGC+AGG 0.578532 7_2:-52403773 Msa1061930:CDS
AGCTTTGACCAGATTTGCAG+TGG 0.580640 7_2:-52403215 Msa1061930:three_prime_UTR
CGCAAAGGTTCATCAAGCCA+AGG 0.586184 7_2:-52405201 Msa1061930:CDS
GTAAACTAAGGTGGACAGGT+TGG 0.586976 7_2:+52403170 None:intergenic
CAATGAAATCAAGCTTCCAC+AGG 0.589243 7_2:-52403730 Msa1061930:CDS
AATGCTACCAAGCAAATCAA+AGG 0.589835 7_2:+52403664 None:intergenic
TGATAATGAGAAAGTGATTG+TGG 0.597158 7_2:+52405000 None:intergenic
TATGGTAGCAGTACTGGCAC+TGG 0.599660 7_2:-52404620 Msa1061930:CDS
AATGACTCCAAATGCCGCAA+AGG 0.600691 7_2:-52405216 Msa1061930:CDS
CATCAGTACGTCCACTCCTG+TGG 0.602987 7_2:+52403714 None:intergenic
GATGCAAGTGTTGTTGCGGC+TGG 0.606152 7_2:+52404069 None:intergenic
AGGCTGAACACAATAAGGGC+GGG 0.606490 7_2:-52404845 Msa1061930:CDS
CAGCAAAGACAAACAAATGG+AGG 0.610080 7_2:-52404794 Msa1061930:CDS
GGTCATTAGAAAGTCTCTCA+AGG 0.612790 7_2:-52403362 Msa1061930:three_prime_UTR
TCATAATAAGTAAACTAAGG+TGG 0.614460 7_2:+52403161 None:intergenic
ATTACCGAAATGCGAAACAT+GGG 0.619209 7_2:+52405833 None:intergenic
ATTGTTGTTATGATTCTCAG+AGG 0.625609 7_2:+52405384 None:intergenic
TTTGGAATCAACATGGAGAG+AGG 0.627829 7_2:+52405408 None:intergenic
GGCAACAACAAATTGCTGAA+AGG 0.630304 7_2:-52404865 Msa1061930:CDS
GAAGCAGCAAGAGAATCGAT+TGG 0.636063 7_2:+52405496 None:intergenic
TTTGGAAGGTAAAGTAACAG+GGG 0.638650 7_2:+52405053 None:intergenic
GGGTATGCAGAAGCAAAATG+GGG 0.639834 7_2:-52404825 Msa1061930:CDS
CAGTTTCTCTCCGACGACGA+CGG 0.644936 7_2:-52405628 Msa1061930:CDS
AAACGATGCAAGTGTTGTTG+CGG 0.650274 7_2:+52404065 None:intergenic
AAAGCACATAGGGTTGATTG+TGG 0.656361 7_2:+52405243 None:intergenic
ATGGCTGAGAATTTGGATGA+CGG 0.669713 7_2:-52405670 Msa1061930:CDS
AAGGCTGAACACAATAAGGG+CGG 0.671077 7_2:-52404846 Msa1061930:CDS
GGTATGCAGAAGCAAAATGG+GGG 0.674755 7_2:-52404824 Msa1061930:CDS
GTTGCACCTCCAAGGCATAG+GGG 0.688695 7_2:-52403822 Msa1061930:CDS
AGCTTGAAGACTATATAGTG+GGG 0.692039 7_2:-52404094 Msa1061930:CDS
TCGTCGGAGAGAAACTGCGG+CGG 0.707718 7_2:+52405634 None:intergenic
TCGTCGTCGGAGAGAAACTG+CGG 0.728597 7_2:+52405631 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATAATAATAATAATAAAT+TGG - chr7_2:52405738-52405757 Msa1061930:five_prime_UTR 0.0%
!!! GCATTTTTTTTTTTTTTTTT+TGG - chr7_2:52403657-52403676 Msa1061930:three_prime_UTR 10.0%
!! TGTTTATTTATTTAATCAAA+AGG - chr7_2:52405111-52405130 Msa1061930:CDS 10.0%
!! GTTTATTTATTTAATCAAAA+GGG - chr7_2:52405112-52405131 Msa1061930:CDS 10.0%
!! TTTATTTATTTAATCAAAAG+GGG - chr7_2:52405113-52405132 Msa1061930:CDS 10.0%
!! TAATAATAATAGAAGATAAA+TGG - chr7_2:52405498-52405517 Msa1061930:CDS 10.0%
!!! TTTTGTTTATTTTTTTTGTT+TGG - chr7_2:52405698-52405717 Msa1061930:five_prime_UTR 10.0%
!! AAATTATTAAATATCACAAC+CGG + chr7_2:52404748-52404767 None:intergenic 15.0%
!! CAACTTAATTAATGTAATTA+AGG + chr7_2:52405014-52405033 None:intergenic 15.0%
!!! AAACAAAATCTAGTTTATAA+GGG + chr7_2:52405064-52405083 None:intergenic 15.0%
!!! AAAACAAAATCTAGTTTATA+AGG + chr7_2:52405065-52405084 None:intergenic 15.0%
!! TTATTTATTTAATCAAAAGG+GGG - chr7_2:52405114-52405133 Msa1061930:CDS 15.0%
!!! TTTTTTTGTTTGGTTTTATT+AGG - chr7_2:52405708-52405727 Msa1061930:five_prime_UTR 15.0%
!!! TTTTTTGTTTGGTTTTATTA+GGG - chr7_2:52405709-52405728 Msa1061930:five_prime_UTR 15.0%
!!! AATAATAATAAATTGGTTTG+TGG - chr7_2:52405745-52405764 Msa1061930:five_prime_UTR 15.0%
!! TATTCATAATAAGTAAACTA+AGG + chr7_2:52405853-52405872 None:intergenic 15.0%
!! AAAAATAATAAAACCCCAAA+AGG + chr7_2:52403688-52403707 None:intergenic 20.0%
!! AAAATTAACATCATCATCAT+GGG + chr7_2:52404481-52404500 None:intergenic 20.0%
!!! AATGTTTTAATGCATTTAAG+AGG - chr7_2:52404553-52404572 Msa1061930:intron 20.0%
!!! ATGTTTTAATGCATTTAAGA+GGG - chr7_2:52404554-52404573 Msa1061930:intron 20.0%
!!! TTTAAAATGTTACACAACAA+TGG - chr7_2:52404603-52404622 Msa1061930:CDS 20.0%
!!! TTTTAATTGTTTTGAATGTG+TGG - chr7_2:52404721-52404740 Msa1061930:CDS 20.0%
!!! AACTTTACCAATTTTTTTTG+TGG - chr7_2:52404825-52404844 Msa1061930:CDS 20.0%
!! ACAAAAACCACAAAAAAAAT+TGG + chr7_2:52404835-52404854 None:intergenic 20.0%
!! ATGATGAAAATGATATGAAA+TGG - chr7_2:52405320-52405339 Msa1061930:intron 20.0%
!!! AGTTTTATAGAAGAAACAAT+AGG - chr7_2:52405419-52405438 Msa1061930:CDS 20.0%
!!! TTTTTTGGTTGTTAAAATGA+AGG - chr7_2:52405444-52405463 Msa1061930:CDS 20.0%
!!! TTGTTAAAATGAAGGTTTTT+TGG - chr7_2:52405452-52405471 Msa1061930:CDS 20.0%
! CAAAAGAAAAAGAGATTATG+TGG + chr7_2:52403212-52403231 None:intergenic 25.0%
! GAAATTGAAGTGAAAGAAAA+GGG + chr7_2:52403285-52403304 None:intergenic 25.0%
! GAATCATAACAACAATAACT+TGG - chr7_2:52403631-52403650 Msa1061930:three_prime_UTR 25.0%
!!! TTTTTTTTGGGTTTTCCTTT+TGG - chr7_2:52403670-52403689 Msa1061930:three_prime_UTR 25.0%
!!! TTTTTTTGGGTTTTCCTTTT+GGG - chr7_2:52403671-52403690 Msa1061930:three_prime_UTR 25.0%
! AGAAATTCAACAAAAAAACC+AGG - chr7_2:52404439-52404458 Msa1061930:intron 25.0%
! CAAAATTAACATCATCATCA+TGG + chr7_2:52404482-52404501 None:intergenic 25.0%
!!! AAGAAACAATAGGTCTTTTT+TGG - chr7_2:52405429-52405448 Msa1061930:CDS 25.0%
! AAGAGGCTAAAAATAATGTT+AGG - chr7_2:52405534-52405553 Msa1061930:CDS 25.0%
!!! TGTTTTTTTCTTAATCCAAG+AGG + chr7_2:52405562-52405581 None:intergenic 25.0%
!! ACTTTTTGTTCTTTTTGATG+AGG - chr7_2:52405624-52405643 Msa1061930:CDS 25.0%
!! CTTTTTGTTCTTTTTGATGA+GGG - chr7_2:52405625-52405644 Msa1061930:CDS 25.0%
! GACAGAAAAAATTAAGAAGA+AGG - chr7_2:52405770-52405789 Msa1061930:five_prime_UTR 25.0%
! TCATAATAAGTAAACTAAGG+TGG + chr7_2:52405850-52405869 None:intergenic 25.0%
AGAAAAAGAGATTATGTGGA+GGG + chr7_2:52403208-52403227 None:intergenic 30.0%
AAGAAAAAGAGATTATGTGG+AGG + chr7_2:52403209-52403228 None:intergenic 30.0%
GGAAATTGAAGTGAAAGAAA+AGG + chr7_2:52403286-52403305 None:intergenic 30.0%
ACAGAGAGAAAAAAAAACAG+AGG + chr7_2:52403307-52403326 None:intergenic 30.0%
! ATTGTTGTTATGATTCTCAG+AGG + chr7_2:52403627-52403646 None:intergenic 30.0%
!!! TTTTTTGGGTTTTCCTTTTG+GGG - chr7_2:52403672-52403691 Msa1061930:three_prime_UTR 30.0%
!!! GTTTTTATTGTCGTTTTGCA+GGG - chr7_2:52403735-52403754 Msa1061930:CDS 30.0%
!! TTTTGGAAGGTAAAGTAACA+GGG + chr7_2:52403959-52403978 None:intergenic 30.0%
!! TTTTTGGAAGGTAAAGTAAC+AGG + chr7_2:52403960-52403979 None:intergenic 30.0%
! TGATAATGAGAAAGTGATTG+TGG + chr7_2:52404011-52404030 None:intergenic 30.0%
TTTCAGCAAAGACAAACAAA+TGG - chr7_2:52404211-52404230 Msa1061930:intron 30.0%
GTTGAATTTCTTGATTCAAC+AGG + chr7_2:52404430-52404449 None:intergenic 30.0%
GGAAAAAAAAAGTAATCACC+TGG + chr7_2:52404460-52404479 None:intergenic 30.0%
!!! TGTACGTTTTTTTGCGTTTT+TGG - chr7_2:52404526-52404545 Msa1061930:intron 30.0%
! TTTTAATGCATTTAAGAGGG+TGG - chr7_2:52404557-52404576 Msa1061930:intron 30.0%
CTAAGTCATAAACGAAAAAC+GGG + chr7_2:52404671-52404690 None:intergenic 30.0%
CCTAAGTCATAAACGAAAAA+CGG + chr7_2:52404672-52404691 None:intergenic 30.0%
! TTAATGTACGTTTTTGCTAC+TGG - chr7_2:52404698-52404717 Msa1061930:CDS 30.0%
CTATATAGTCTTCAAGCTTT+AGG + chr7_2:52404912-52404931 None:intergenic 30.0%
AAAGCTTGAAGACTATATAG+TGG - chr7_2:52404912-52404931 Msa1061930:intron 30.0%
AAGCTTGAAGACTATATAGT+GGG - chr7_2:52404913-52404932 Msa1061930:intron 30.0%
! AGTACCATTTTCAACATTAG+AGG + chr7_2:52404977-52404996 None:intergenic 30.0%
!! ATTTGCTTGGTAGCATTTTA+AGG - chr7_2:52405350-52405369 Msa1061930:intron 30.0%
!! TTTGCTTGGTAGCATTTTAA+GGG - chr7_2:52405351-52405370 Msa1061930:intron 30.0%
!!! ATGGTTTTTAGTTTAGCAAG+AGG - chr7_2:52405517-52405536 Msa1061930:CDS 30.0%
ATTACCGAAATGCGAAACAT+GGG + chr7_2:52403178-52403197 None:intergenic 35.0%
TTTCGCATTTCGGTAATCAT+GGG - chr7_2:52403181-52403200 Msa1061930:three_prime_UTR 35.0%
!!! GGATTTTGGTTAGGTTTGTT+AGG + chr7_2:52403247-52403266 None:intergenic 35.0%
!!! AATGTGAAGGGATTTTGGTT+AGG + chr7_2:52403256-52403275 None:intergenic 35.0%
! GAGAAAATGTGAAGGGATTT+TGG + chr7_2:52403261-52403280 None:intergenic 35.0%
AAAGGGAGAGAAAATGTGAA+GGG + chr7_2:52403268-52403287 None:intergenic 35.0%
AAAAGGGAGAGAAAATGTGA+AGG + chr7_2:52403269-52403288 None:intergenic 35.0%
! CTCTCTGTTTTCATTTTCCA+TGG - chr7_2:52403319-52403338 Msa1061930:three_prime_UTR 35.0%
! ATTTTCCATGGCTGAGAATT+TGG - chr7_2:52403331-52403350 Msa1061930:three_prime_UTR 35.0%
AATCCATAAGGAAAAAGCAG+AGG + chr7_2:52403470-52403489 None:intergenic 35.0%
! TTGTTCATCATCGTCACTTT+CGG + chr7_2:52403546-52403565 None:intergenic 35.0%
!!! CGTTTTTATTGTCGTTTTGC+AGG - chr7_2:52403734-52403753 Msa1061930:CDS 35.0%
! GTCATTTCCAAAAGCACATA+GGG + chr7_2:52403778-52403797 None:intergenic 35.0%
! AGTCATTTCCAAAAGCACAT+AGG + chr7_2:52403779-52403798 None:intergenic 35.0%
CATTCTCACAAACACCATTA+GGG + chr7_2:52403836-52403855 None:intergenic 35.0%
TTTGGAAGGTAAAGTAACAG+GGG + chr7_2:52403958-52403977 None:intergenic 35.0%
CAAAAAAGCTCAAATCAGCA+GGG + chr7_2:52403986-52404005 None:intergenic 35.0%
!!! GTTGATGATTTTAGTTGCAG+AGG - chr7_2:52404116-52404135 Msa1061930:CDS 35.0%
!!! GATTTTAGTTGCAGAGGATT+AGG - chr7_2:52404122-52404141 Msa1061930:CDS 35.0%
TGAAAGGCTGAACACAATAA+GGG - chr7_2:52404159-52404178 Msa1061930:intron 35.0%
TAGGTTTGTCATCATCTTCA+TGG - chr7_2:52404287-52404306 Msa1061930:intron 35.0%
! GTTTTCCTTGGAAATGGTTA+TGG - chr7_2:52404370-52404389 Msa1061930:intron 35.0%
!! CCGTTTTTCGTTTATGACTT+AGG - chr7_2:52404669-52404688 Msa1061930:CDS 35.0%
!!! ATTGTTTTGAATGTGTGGAC+CGG - chr7_2:52404726-52404745 Msa1061930:CDS 35.0%
!! GTCTTCAAGCTTTAGGTTAA+GGG + chr7_2:52404905-52404924 None:intergenic 35.0%
!! AGTCTTCAAGCTTTAGGTTA+AGG + chr7_2:52404906-52404925 None:intergenic 35.0%
AGCTTGAAGACTATATAGTG+GGG - chr7_2:52404914-52404933 Msa1061930:intron 35.0%
TCCACCTCTAATGTTGAAAA+TGG - chr7_2:52404970-52404989 Msa1061930:intron 35.0%
! ACCATTTTCAACATTAGAGG+TGG + chr7_2:52404974-52404993 None:intergenic 35.0%
!! AAATGGTCCTTTGATTTGCT+TGG - chr7_2:52405337-52405356 Msa1061930:intron 35.0%
AATGCTACCAAGCAAATCAA+AGG + chr7_2:52405347-52405366 None:intergenic 35.0%
!! TGGTAGCATTTTAAGGGATT+TGG - chr7_2:52405357-52405376 Msa1061930:intron 35.0%
!! GGTAGCATTTTAAGGGATTT+GGG - chr7_2:52405358-52405377 Msa1061930:intron 35.0%
!! GTAGCATTTTAAGGGATTTG+GGG - chr7_2:52405359-52405378 Msa1061930:intron 35.0%
!! TAGCATTTTAAGGGATTTGG+GGG - chr7_2:52405360-52405379 Msa1061930:intron 35.0%
AGGGGATTTGATGTAACAAA+AGG - chr7_2:52405391-52405410 Msa1061930:CDS 35.0%
!!! GGTTTTTTGGATGAAGATGT+AGG - chr7_2:52405465-52405484 Msa1061930:CDS 35.0%
AAATAATGTTAGGAGCCTCT+TGG - chr7_2:52405544-52405563 Msa1061930:CDS 35.0%
ACTGATATCACTGCAATTTC+AGG + chr7_2:52405603-52405622 None:intergenic 35.0%
CTCTCAAGGAAAGACAAAAA+TGG - chr7_2:52405660-52405679 Msa1061930:CDS 35.0%
TCAAGGAAAGACAAAAATGG+TGG - chr7_2:52405663-52405682 Msa1061930:CDS 35.0%
ATAAGTAAACTAAGGTGGAC+AGG + chr7_2:52405845-52405864 None:intergenic 35.0%
GATTACCGAAATGCGAAACA+TGG + chr7_2:52403179-52403198 None:intergenic 40.0%
GTTTCGCATTTCGGTAATCA+TGG - chr7_2:52403180-52403199 Msa1061930:three_prime_UTR 40.0%
ATGGCTGAGAATTTGGATGA+CGG - chr7_2:52403338-52403357 Msa1061930:three_prime_UTR 40.0%
TTGAAAAGCGAAAGCGAAGA+AGG + chr7_2:52403434-52403453 None:intergenic 40.0%
!! TCGCTTTCGCTTTTCAACAA+TGG - chr7_2:52403437-52403456 Msa1061930:three_prime_UTR 40.0%
!!! GATCCTCTGCTTTTTCCTTA+TGG - chr7_2:52403464-52403483 Msa1061930:three_prime_UTR 40.0%
TCAGAACAAGCGAATCCATA+AGG + chr7_2:52403482-52403501 None:intergenic 40.0%
! TTTGGAATCAACATGGAGAG+AGG + chr7_2:52403603-52403622 None:intergenic 40.0%
GTTATGATTCTCAGAGGCTT+TGG + chr7_2:52403621-52403640 None:intergenic 40.0%
!!! TTTATTGTCGTTTTGCAGGG+AGG - chr7_2:52403738-52403757 Msa1061930:CDS 40.0%
! AAAGCACATAGGGTTGATTG+TGG + chr7_2:52403768-52403787 None:intergenic 40.0%
! CAATCAACCCTATGTGCTTT+TGG - chr7_2:52403768-52403787 Msa1061930:CDS 40.0%
GCATTCTCACAAACACCATT+AGG + chr7_2:52403837-52403856 None:intergenic 40.0%
GGGGAAGAAGAATTTCTTTG+AGG + chr7_2:52403939-52403958 None:intergenic 40.0%
!! CAAATCAGCAGGGTTGTTTT+TGG + chr7_2:52403976-52403995 None:intergenic 40.0%
GCAAAAAAGCTCAAATCAGC+AGG + chr7_2:52403987-52404006 None:intergenic 40.0%
GGCAACAACAAATTGCTGAA+AGG - chr7_2:52404143-52404162 Msa1061930:CDS 40.0%
CTGAAAGGCTGAACACAATA+AGG - chr7_2:52404158-52404177 Msa1061930:intron 40.0%
CAGCAAAGACAAACAAATGG+AGG - chr7_2:52404214-52404233 Msa1061930:intron 40.0%
GGAGGAAGAAATGTTGTTGA+TGG - chr7_2:52404232-52404251 Msa1061930:intron 40.0%
AACAACAACAACAACGTTGC+TGG - chr7_2:52404262-52404281 Msa1061930:intron 40.0%
!! AACAACAACGTTGCTGGTTT+AGG - chr7_2:52404268-52404287 Msa1061930:intron 40.0%
TGTTGAGCATGTTGTAGTGT+TGG + chr7_2:52404313-52404332 None:intergenic 40.0%
AGGAAAACAGCTCTCATGTA+GGG + chr7_2:52404358-52404377 None:intergenic 40.0%
AAGGAAAACAGCTCTCATGT+AGG + chr7_2:52404359-52404378 None:intergenic 40.0%
!! TACATGAGAGCTGTTTTCCT+TGG - chr7_2:52404358-52404377 Msa1061930:intron 40.0%
!! AGAGCTGTTTTCCTTGGAAA+TGG - chr7_2:52404364-52404383 Msa1061930:intron 40.0%
TGCTACCATAACCATTTCCA+AGG + chr7_2:52404378-52404397 None:intergenic 40.0%
AATGGTTATGGTAGCAGTAC+TGG - chr7_2:52404382-52404401 Msa1061930:intron 40.0%
TTTCTTGATTCAACAGGACG+AGG + chr7_2:52404424-52404443 None:intergenic 40.0%
! AAACGATGCAAGTGTTGTTG+CGG + chr7_2:52404946-52404965 None:intergenic 40.0%
ACATTATTACCCCTATGCCT+TGG + chr7_2:52405198-52405217 None:intergenic 40.0%
CAATGAAATCAAGCTTCCAC+AGG - chr7_2:52405278-52405297 Msa1061930:intron 40.0%
!!! GGATTTGGGGGAGTATTTTA+GGG - chr7_2:52405372-52405391 Msa1061930:intron 40.0%
!!! GATTTGGGGGAGTATTTTAG+GGG - chr7_2:52405373-52405392 Msa1061930:intron 40.0%
GGTCATTAGAAAGTCTCTCA+AGG - chr7_2:52405646-52405665 Msa1061930:CDS 40.0%
AGGAAAGACAAAAATGGTGG+TGG - chr7_2:52405666-52405685 Msa1061930:CDS 40.0%
!! AAGACAAAAATGGTGGTGGA+TGG - chr7_2:52405670-52405689 Msa1061930:CDS 40.0%
!! AGACAAAAATGGTGGTGGAT+GGG - chr7_2:52405671-52405690 Msa1061930:CDS 40.0%
CTGTAATACCACTGCAAATC+TGG + chr7_2:52405804-52405823 None:intergenic 40.0%
CTCTCCCATGTTTCGCATTT+CGG - chr7_2:52403171-52403190 Msa1061930:three_prime_UTR 45.0%
GTCATCCAAATTCTCAGCCA+TGG + chr7_2:52403339-52403358 None:intergenic 45.0%
! ATTTGGATGACGGTGAGTTC+TGG - chr7_2:52403348-52403367 Msa1061930:three_prime_UTR 45.0%
!! AGAAGGTTTCGGTTTTGTGG+TGG + chr7_2:52403417-52403436 None:intergenic 45.0%
! CGAAGAAGGTTTCGGTTTTG+TGG + chr7_2:52403420-52403439 None:intergenic 45.0%
AGCGAAAGCGAAGAAGGTTT+CGG + chr7_2:52403428-52403447 None:intergenic 45.0%
GAAGCAGCAAGAGAATCGAT+TGG + chr7_2:52403515-52403534 None:intergenic 45.0%
!! CAGAGGCTTTGGAATCAACA+TGG + chr7_2:52403610-52403629 None:intergenic 45.0%
AATGACTCCAAATGCCGCAA+AGG - chr7_2:52403792-52403811 Msa1061930:CDS 45.0%
TGATGAACCTTTGCGGCATT+TGG + chr7_2:52403802-52403821 None:intergenic 45.0%
! CTTGGCTTGATGAACCTTTG+CGG + chr7_2:52403809-52403828 None:intergenic 45.0%
AAACACCATTAGGGCTTCCT+TGG + chr7_2:52403827-52403846 None:intergenic 45.0%
!!! TCAGCAGGGTTGTTTTTGGA+AGG + chr7_2:52403972-52403991 None:intergenic 45.0%
AAGGCTGAACACAATAAGGG+CGG - chr7_2:52404162-52404181 Msa1061930:intron 45.0%
CGGGTATGCAGAAGCAAAAT+GGG - chr7_2:52404182-52404201 Msa1061930:intron 45.0%
GGGTATGCAGAAGCAAAATG+GGG - chr7_2:52404183-52404202 Msa1061930:intron 45.0%
GGTATGCAGAAGCAAAATGG+GGG - chr7_2:52404184-52404203 Msa1061930:intron 45.0%
AGGGTTGAGGAGCTTGAAAT+TGG + chr7_2:52404339-52404358 None:intergenic 45.0%
! TGTAGCTTGTGCAACTGCAT+TGG - chr7_2:52404858-52404877 Msa1061930:CDS 45.0%
TTATTACCCCTATGCCTTGG+AGG + chr7_2:52405195-52405214 None:intergenic 45.0%
ATTTCATTGCTCACTGCTGC+TGG + chr7_2:52405267-52405286 None:intergenic 45.0%
AAATCAAGCTTCCACAGGAG+TGG - chr7_2:52405283-52405302 Msa1061930:intron 45.0%
!!! GGGATTTGGGGGAGTATTTT+AGG - chr7_2:52405371-52405390 Msa1061930:intron 45.0%
! AGCTTTGACCAGATTTGCAG+TGG - chr7_2:52405793-52405812 Msa1061930:five_prime_UTR 45.0%
GTAAACTAAGGTGGACAGGT+TGG + chr7_2:52405841-52405860 None:intergenic 45.0%
!!! CATTTTTTTTTTTTTTTTTT+GGG - chr7_2:52403658-52403677 Msa1061930:three_prime_UTR 5.0%
!!! TTTATTATTTTTTTACTTTT+AGG - chr7_2:52403696-52403715 Msa1061930:three_prime_UTR 5.0%
!!! TTATTATTTTTTTACTTTTA+GGG - chr7_2:52403697-52403716 Msa1061930:exon 5.0%
!!! AATTTCATATTTTTTAATAA+TGG - chr7_2:52404066-52404085 Msa1061930:CDS 5.0%
!!! ATTTCATATTTTTTAATAAT+GGG - chr7_2:52404067-52404086 Msa1061930:CDS 5.0%
CGCAAAGGTTCATCAAGCCA+AGG - chr7_2:52403807-52403826 Msa1061930:CDS 50.0%
TCAAGCCAAGGAAGCCCTAA+TGG - chr7_2:52403819-52403838 Msa1061930:CDS 50.0%
AGGCTGAACACAATAAGGGC+GGG - chr7_2:52404163-52404182 Msa1061930:intron 50.0%
GCGGGTATGCAGAAGCAAAA+TGG - chr7_2:52404181-52404200 Msa1061930:intron 50.0%
ACAGCTCTCATGTAGGGTTG+AGG + chr7_2:52404352-52404371 None:intergenic 50.0%
!! TATGGTAGCAGTACTGGCAC+TGG - chr7_2:52404388-52404407 Msa1061930:intron 50.0%
!! TGCATTGGTTCCAGCTAGAG+TGG - chr7_2:52404873-52404892 Msa1061930:CDS 50.0%
TAGCTGTTGTTGCACCTCCA+AGG - chr7_2:52405178-52405197 Msa1061930:CDS 50.0%
TTGTTGCACCTCCAAGGCAT+AGG - chr7_2:52405184-52405203 Msa1061930:CDS 50.0%
TGTTGCACCTCCAAGGCATA+GGG - chr7_2:52405185-52405204 Msa1061930:CDS 50.0%
! GGACAGGTTGGCATCAAAGA+AGG + chr7_2:52405829-52405848 None:intergenic 50.0%
TCGTCGTCGGAGAGAAACTG+CGG + chr7_2:52403380-52403399 None:intergenic 55.0%
CAGTTTCTCTCCGACGACGA+CGG - chr7_2:52403380-52403399 Msa1061930:three_prime_UTR 55.0%
TGTGTCATCACCGTCGTCGT+CGG + chr7_2:52403393-52403412 None:intergenic 55.0%
! GATGCAAGTGTTGTTGCGGC+TGG + chr7_2:52404942-52404961 None:intergenic 55.0%
GTTGCACCTCCAAGGCATAG+GGG - chr7_2:52405186-52405205 Msa1061930:CDS 55.0%
GCAGCAGCAACAATACCAGC+AGG - chr7_2:52405235-52405254 Msa1061930:CDS 55.0%
CATCAGTACGTCCACTCCTG+TGG + chr7_2:52405297-52405316 None:intergenic 55.0%
TCGTCGGAGAGAAACTGCGG+CGG + chr7_2:52403377-52403396 None:intergenic 60.0%
! TTGCTCACTGCTGCTGGCTG+AGG + chr7_2:52405261-52405280 None:intergenic 60.0%
AGGGCATGCGCCACTCTAGC+TGG + chr7_2:52404886-52404905 None:intergenic 65.0%
! TGCTGCTGGCTGAGGCCTGC+TGG + chr7_2:52405253-52405272 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr7_2 gene 52403148 52405882 52403148 ID=Msa1061930;Name=Msa1061930
chr7_2 mRNA 52403148 52405882 52403148 ID=Msa1061930-mRNA-1;Parent=Msa1061930;Name=Msa1061930-mRNA-1;_AED=0.12;_eAED=0.12;_QI=190|1|1|1|0.8|0.66|6|545|400
chr7_2 exon 52403148 52403556 52403148 ID=Msa1061930-mRNA-1:exon:9480;Parent=Msa1061930-mRNA-1
chr7_2 exon 52403580 52403852 52403580 ID=Msa1061930-mRNA-1:exon:9479;Parent=Msa1061930-mRNA-1
chr7_2 exon 52404039 52404167 52404039 ID=Msa1061930-mRNA-1:exon:9478;Parent=Msa1061930-mRNA-1
chr7_2 exon 52404570 52404900 52404570 ID=Msa1061930-mRNA-1:exon:9477;Parent=Msa1061930-mRNA-1
chr7_2 exon 52404984 52405274 52404984 ID=Msa1061930-mRNA-1:exon:9476;Parent=Msa1061930-mRNA-1
chr7_2 exon 52405378 52405882 52405378 ID=Msa1061930-mRNA-1:exon:9475;Parent=Msa1061930-mRNA-1
chr7_2 five_prime_UTR 52405693 52405882 52405693 ID=Msa1061930-mRNA-1:five_prime_utr;Parent=Msa1061930-mRNA-1
chr7_2 CDS 52405378 52405692 52405378 ID=Msa1061930-mRNA-1:cds;Parent=Msa1061930-mRNA-1
chr7_2 CDS 52404984 52405274 52404984 ID=Msa1061930-mRNA-1:cds;Parent=Msa1061930-mRNA-1
chr7_2 CDS 52404570 52404900 52404570 ID=Msa1061930-mRNA-1:cds;Parent=Msa1061930-mRNA-1
chr7_2 CDS 52404039 52404167 52404039 ID=Msa1061930-mRNA-1:cds;Parent=Msa1061930-mRNA-1
chr7_2 CDS 52403716 52403852 52403716 ID=Msa1061930-mRNA-1:cds;Parent=Msa1061930-mRNA-1
chr7_2 three_prime_UTR 52403580 52403715 52403580 ID=Msa1061930-mRNA-1:three_prime_utr;Parent=Msa1061930-mRNA-1
chr7_2 three_prime_UTR 52403148 52403556 52403148 ID=Msa1061930-mRNA-1:three_prime_utr;Parent=Msa1061930-mRNA-1
Gene Sequence

>Msa1061930

ATGGCTGAGAATTTGGATGACGGTGAGTTCTGGCTTCCGCCGCAGTTTCTCTCCGACGACGACGGTGATGACACAACCACCACAAAACCGAAACCTTCTTCGCTTTCGCTTTTCAACAATGGTGAAGATCCTCTGCTTTTTCCTTATGGATTCGCTTGTTCTGATCTCAGTTCACCAATCGATTCTCTTGCTGCTTCAAGCGAAACCGAAAGTGACGATGATGAACAACTCTTTGCTGAGTTAACTCGTTGCTTGTCTCGTTCCTCTCTCCATGTTGATTCCAAAGCCTCTGAGAATCATAACAACAATAACTTGGGAGGTTCACCACAATCAACCCTATGTGCTTTTGGAAATGACTCCAAATGCCGCAAAGGTTCATCAAGCCAAGGAAGCCCTAATGGTGTTTGTGAGAATGCTTCATCGAATTCTACTTTGAATCTGTTGCATGCTGCTGCTGATGAAGTTGAAACTTTGAGATTGAATCAACAAACTGTTCCTCAAAGAAATTCTTCTTCCCCTGTTACTTTACCTTCCAAAAACAACCCTGCTGATTTGAGCTTTTTTGCTCCACAATCACTTTCTCATTATCAATTGCAAATTGCTAATTTGCAGAGGATTAGGCAACAACAAATTGCTGAAAGGCTGAACACAATAAGGGCGGGTATGCAGAAGCAAAATGGGGGTGCATTTCAGCAAAGACAAACAAATGGAGGAAGAAATGTTGTTGATGGAAGAAACAACAACAACAACAACGTTGCTGGTTTAGGTTTGTCATCATCTTCATGGCCAACACTACAACATGCTCAACAAAACCAATTTCAAGCTCCTCAACCCTACATGAGAGCTGTTTTCCTTGGAAATGGTTATGGTAGCAGTACTGGCACTGGTGTGTTCTTACCTCGTCCTGTTGAATCAAGAAATTCAACAAAAAAACCAGCTTGTGCAACTGCATTGGTTCCAGCTAGAGTGGCGCATGCCCTTAACCTAAAGCTTGAAGACTATATAGTGGGGTGCCAGCCGCAACAACACTTGCATCGTTTTAATTCCACCTCTAATGTTGAAAATGTAGCTGTTGTTGCACCTCCAAGGCATAGGGGTAATAATGTCAATTCACAAAAGAAACGCAGCAGCAACAATACCAGCAGGCCTCAGCCAGCAGCAGTGAGCAATGAAATCAAGCTTCCACAGGAGTGGACGTACTGA
Protein sequence

>Msa1061930

MAENLDDGEFWLPPQFLSDDDGDDTTTTKPKPSSLSLFNNGEDPLLFPYGFACSDLSSPIDSLAASSETESDDDEQLFAELTRCLSRSSLHVDSKASENHNNNNLGGSPQSTLCAFGNDSKCRKGSSSQGSPNGVCENASSNSTLNLLHAAADEVETLRLNQQTVPQRNSSSPVTLPSKNNPADLSFFAPQSLSHYQLQIANLQRIRQQQIAERLNTIRAGMQKQNGGAFQQRQTNGGRNVVDGRNNNNNNVAGLGLSSSSWPTLQHAQQNQFQAPQPYMRAVFLGNGYGSSTGTGVFLPRPVESRNSTKKPACATALVPARVAHALNLKLEDYIVGCQPQQHLHRFNSTSNVENVAVVAPPRHRGNNVNSQKKRSSNNTSRPQPAAVSNEIKLPQEWTY*