Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1078000 | AES79914.2 | 93.750 | 112 | 7 | 0 | 1 | 112 | 1 | 112 | 4.79e-60 | 198 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1078000 | sp|Q9ZVI9|PECT1_ARATH | 85.714 | 63 | 9 | 0 | 50 | 112 | 50 | 112 | 2.54e-34 | 125 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1078000 | G7KZ98 | 93.750 | 112 | 7 | 0 | 1 | 112 | 1 | 112 | 2.29e-60 | 198 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0004540 | Msa1078000 | 0.807106 | 5.943610e-50 | -8.615850e-47 |
| Msa0005000 | Msa1078000 | -0.807199 | 5.679612e-50 | -8.615850e-47 |
| Msa0041690 | Msa1078000 | 0.805592 | 1.239272e-49 | -8.615850e-47 |
| Msa0098390 | Msa1078000 | -0.810081 | 1.376169e-50 | -8.615850e-47 |
| Msa0639120 | Msa1078000 | 0.805683 | 1.186127e-49 | -8.615850e-47 |
| Msa0658480 | Msa1078000 | 0.821036 | 4.999131e-53 | -8.615850e-47 |
| Msa0687230 | Msa1078000 | 0.828212 | 1.019347e-54 | -8.615850e-47 |
| Msa0734960 | Msa1078000 | 0.833496 | 5.157789e-56 | -8.615850e-47 |
| Msa0749400 | Msa1078000 | 0.801766 | 7.712627e-49 | -8.615850e-47 |
| Msa0756930 | Msa1078000 | 0.816031 | 6.817315e-52 | -8.615850e-47 |
| Msa0771090 | Msa1078000 | 0.800163 | 1.639761e-48 | -8.615850e-47 |
| Msa0826510 | Msa1078000 | 0.811385 | 7.189698e-51 | -8.615850e-47 |
| Msa0836500 | Msa1078000 | 0.808568 | 2.904930e-50 | -8.615850e-47 |
| Msa0127040 | Msa1078000 | 0.801355 | 9.365256e-49 | -8.615850e-47 |
| Msa0130240 | Msa1078000 | 0.818680 | 1.727240e-52 | -8.615850e-47 |
| Msa0229940 | Msa1078000 | 0.804570 | 2.027171e-49 | -8.615850e-47 |
| Msa0432070 | Msa1078000 | 0.802522 | 5.390260e-49 | -8.615850e-47 |
| Msa0478580 | Msa1078000 | 0.801470 | 8.868674e-49 | -8.615850e-47 |
| Msa0980940 | Msa1078000 | 0.831896 | 1.287019e-55 | -8.615850e-47 |
| Msa1051600 | Msa1078000 | 0.806123 | 9.581948e-50 | -8.615850e-47 |
| Msa1078000 | Msa1097230 | 0.800747 | 1.246325e-48 | -8.615850e-47 |
| Msa1078000 | Msa1123350 | 0.804646 | 1.954888e-49 | -8.615850e-47 |
| Msa1078000 | Msa1127540 | 0.828834 | 7.213932e-55 | -8.615850e-47 |
| Msa1078000 | Msa1154540 | 0.802240 | 6.162097e-49 | -8.615850e-47 |
| Msa1078000 | Msa1169720 | 0.801317 | 9.533602e-49 | -8.615850e-47 |
| Msa1078000 | Msa1201830 | 0.808666 | 2.768448e-50 | -8.615850e-47 |
| Msa1078000 | Msa1294840 | 0.805723 | 1.163215e-49 | -8.615850e-47 |
| Msa1078000 | Msa1356610 | 0.817113 | 3.901366e-52 | -8.615850e-47 |
| Msa1078000 | Msa1393800 | 0.812904 | 3.352633e-51 | -8.615850e-47 |
| Msa1078000 | Msa1438450 | 0.820533 | 6.524215e-53 | -8.615850e-47 |
| Msa1078000 | Msa1438640 | 0.809090 | 2.246979e-50 | -8.615850e-47 |
| Msa1078000 | Msa1459910 | 0.811048 | 8.504678e-51 | -8.615850e-47 |
| Msa1078000 | Msa1463470 | 0.804514 | 2.082413e-49 | -8.615850e-47 |
| Msa0322590 | Msa1078000 | 0.821046 | 4.971896e-53 | -8.615850e-47 |
| Msa0343190 | Msa1078000 | 0.808961 | 2.393998e-50 | -8.615850e-47 |
| Msa0484290 | Msa1078000 | 0.805928 | 1.053237e-49 | -8.615850e-47 |
| Msa0516120 | Msa1078000 | 0.805940 | 1.047343e-49 | -8.615850e-47 |
| Msa0529320 | Msa1078000 | 0.830254 | 3.256841e-55 | -8.615850e-47 |
| Msa0549790 | Msa1078000 | 0.816307 | 5.915166e-52 | -8.615850e-47 |
| Msa0560470 | Msa1078000 | 0.810225 | 1.281510e-50 | -8.615850e-47 |
| Msa0850150 | Msa1078000 | 0.806508 | 7.951362e-50 | -8.615850e-47 |
| Msa0865620 | Msa1078000 | 0.807413 | 5.116037e-50 | -8.615850e-47 |
| Msa0869010 | Msa1078000 | 0.807540 | 4.808510e-50 | -8.615850e-47 |
| Msa0888940 | Msa1078000 | 0.827841 | 1.251617e-54 | -8.615850e-47 |
| Msa0943170 | Msa1078000 | 0.801091 | 1.060202e-48 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1078000 | MtrunA17_Chr7g0245591 | 93.750 | 112 | 7 | 0 | 1 | 112 | 1 | 112 | 7.47e-64 | 199 |
| Msa1078000 | MtrunA17_Chr8g0389111 | 68.966 | 58 | 18 | 0 | 55 | 112 | 65 | 122 | 7.50e-25 | 97.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1078000 | AT2G38670.1 | 85.714 | 63 | 9 | 0 | 50 | 112 | 50 | 112 | 2.58e-35 | 125 |
Find 40 sgRNAs with CRISPR-Local
Find 52 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAACCGATTCGTGTTTATA+TGG | 0.236649 | 7_2:+76867522 | Msa1078000:CDS |
| TGATGAAATTATTGCTAATA+AGG | 0.259012 | 7_2:+76867639 | Msa1078000:CDS |
| TATTCATCTTCTGGGCCATT+TGG | 0.267914 | 7_2:+76867466 | Msa1078000:CDS |
| AATTGAACCTTTCATGTAAA+GGG | 0.279295 | 7_2:-76867675 | None:intergenic |
| TCATCGGTGAATGAACAAAA+TGG | 0.285598 | 7_2:+76867357 | Msa1078000:exon |
| CTTCTGGGCCATTTGGGTTG+TGG | 0.345993 | 7_2:+76867473 | Msa1078000:CDS |
| ATCGTTGGTGTATCAGTATT+AGG | 0.355171 | 7_2:+76867439 | Msa1078000:CDS |
| ATTCATCTTCTGGGCCATTT+GGG | 0.367495 | 7_2:+76867467 | Msa1078000:CDS |
| CTTTCATGTAAAGGGGTAAC+AGG | 0.373536 | 7_2:-76867667 | None:intergenic |
| AATGGGGAGGAATAAGAAAA+AGG | 0.380455 | 7_2:-76867243 | None:intergenic |
| CTTGGTGATCAATTGATTGT+TGG | 0.388238 | 7_2:+76867604 | Msa1078000:CDS |
| GTGAAAAGAAGCAAAATATT+CGG | 0.391402 | 7_2:-76867305 | None:intergenic |
| AAATTGAACCTTTCATGTAA+AGG | 0.427115 | 7_2:-76867676 | None:intergenic |
| TTGCGTCAAGCTCGTGCTCT+TGG | 0.428384 | 7_2:+76867586 | Msa1078000:CDS |
| CATCGGTGAATGAACAAAAT+GGG | 0.428841 | 7_2:+76867358 | Msa1078000:exon |
| AATTGAATTGAATTGATGAA+TGG | 0.442664 | 7_2:-76867261 | None:intergenic |
| CCGATTCGTGTTTATATGGA+TGG | 0.449331 | 7_2:+76867526 | Msa1078000:CDS |
| GATGAAATTATTGCTAATAA+GGG | 0.453969 | 7_2:+76867640 | Msa1078000:CDS |
| TCAACAATAATAACCTGAAA+AGG | 0.456418 | 7_2:+76867198 | Msa1078000:five_prime_UTR |
| ATTGAATTGAATTGATGAAT+GGG | 0.473853 | 7_2:-76867260 | None:intergenic |
| CTGTTACCCCTTTACATGAA+AGG | 0.486145 | 7_2:+76867668 | Msa1078000:CDS |
| AACAACGATGTCTGTATCAA+TGG | 0.491664 | 7_2:+76867152 | Msa1078000:five_prime_UTR |
| CCATCCATATAAACACGAAT+CGG | 0.508010 | 7_2:-76867526 | None:intergenic |
| TTGGTCGGAGTTATCATCGT+TGG | 0.530391 | 7_2:+76867424 | Msa1078000:CDS |
| ACGTTTATTCCCTTCGAATG+AGG | 0.532059 | 7_2:+76867326 | Msa1078000:five_prime_UTR |
| CATACGATGGGCTGCCTGTG+TGG | 0.550562 | 7_2:+76867117 | None:intergenic |
| ATCGGTGAATGAACAAAATG+GGG | 0.578092 | 7_2:+76867359 | Msa1078000:exon |
| ATTGAACCTTTCATGTAAAG+GGG | 0.588992 | 7_2:-76867674 | None:intergenic |
| AATCTAATGCGGCGGTGAAG+TGG | 0.605093 | 7_2:+76867401 | Msa1078000:CDS |
| GCGGCGGTGAAGTGGTTGGT+CGG | 0.606525 | 7_2:+76867409 | Msa1078000:CDS |
| TTGAATTGAATTGATGAATG+GGG | 0.607933 | 7_2:-76867259 | None:intergenic |
| TAATGCGGCGGTGAAGTGGT+TGG | 0.623357 | 7_2:+76867405 | Msa1078000:CDS |
| AATTGAATTGATGAATGGGG+AGG | 0.650249 | 7_2:-76867256 | None:intergenic |
| CAGTGTTGAATCTAATGCGG+CGG | 0.663209 | 7_2:+76867393 | Msa1078000:CDS |
| GAATGAGGAAGAAAACTCAT+CGG | 0.665554 | 7_2:+76867341 | Msa1078000:five_prime_UTR |
| CGGTGAATGAACAAAATGGG+GGG | 0.680827 | 7_2:+76867361 | Msa1078000:exon |
| TCATGTAAAGGGGTAACAGG+AGG | 0.682525 | 7_2:-76867664 | None:intergenic |
| TCGGTGAATGAACAAAATGG+GGG | 0.684672 | 7_2:+76867360 | Msa1078000:exon |
| TAGCAGTGTTGAATCTAATG+CGG | 0.691391 | 7_2:+76867390 | Msa1078000:CDS |
| TTGAGACATACAGACCACAC+AGG | 0.735120 | 7_2:-76867131 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTTTTTTTATTTCCTTTTC+AGG | - | chr7_2:76867214-76867233 | None:intergenic | 15.0% |
| !!! | TATATTTAATTTACCATTTC+TGG | + | chr7_2:76867725-76867744 | Msa1078000:intron | 15.0% |
| !! | AGAGTGAAAAAAAATAGAAA+TGG | - | chr7_2:76867179-76867198 | None:intergenic | 20.0% |
| !! | ATTGAATTGAATTGATGAAT+GGG | - | chr7_2:76867263-76867282 | None:intergenic | 20.0% |
| !! | AATTGAATTGAATTGATGAA+TGG | - | chr7_2:76867264-76867283 | None:intergenic | 20.0% |
| !! | TGATGAAATTATTGCTAATA+AGG | + | chr7_2:76867639-76867658 | Msa1078000:CDS | 20.0% |
| !! | GATGAAATTATTGCTAATAA+GGG | + | chr7_2:76867640-76867659 | Msa1078000:CDS | 20.0% |
| ! | TCAACAATAATAACCTGAAA+AGG | + | chr7_2:76867198-76867217 | Msa1078000:five_prime_UTR | 25.0% |
| ! | TTGAATTGAATTGATGAATG+GGG | - | chr7_2:76867262-76867281 | None:intergenic | 25.0% |
| ! | GTGAAAAGAAGCAAAATATT+CGG | - | chr7_2:76867308-76867327 | None:intergenic | 25.0% |
| !! | TTAGGTTTTTATTCATCTTC+TGG | + | chr7_2:76867457-76867476 | Msa1078000:CDS | 25.0% |
| !! | TAGGTTTTTATTCATCTTCT+GGG | + | chr7_2:76867458-76867477 | Msa1078000:CDS | 25.0% |
| !!! | TGTTTTGATATGATGCATTA+TGG | + | chr7_2:76867550-76867569 | Msa1078000:CDS | 25.0% |
| ! | AATTGAACCTTTCATGTAAA+GGG | - | chr7_2:76867678-76867697 | None:intergenic | 25.0% |
| ! | AAATTGAACCTTTCATGTAA+AGG | - | chr7_2:76867679-76867698 | None:intergenic | 25.0% |
| !!! | ATATTCGGTTTTGTTTGGAT+TGG | - | chr7_2:76867293-76867312 | None:intergenic | 30.0% |
| !!! | GCAAAATATTCGGTTTTGTT+TGG | - | chr7_2:76867298-76867317 | None:intergenic | 30.0% |
| !! | AAAACCGATTCGTGTTTATA+TGG | + | chr7_2:76867522-76867541 | Msa1078000:CDS | 30.0% |
| ATTGAACCTTTCATGTAAAG+GGG | - | chr7_2:76867677-76867696 | None:intergenic | 30.0% | |
| ! | AACAGCTTTTTATCCAGAAA+TGG | - | chr7_2:76867741-76867760 | None:intergenic | 30.0% |
| AACAACGATGTCTGTATCAA+TGG | + | chr7_2:76867152-76867171 | Msa1078000:five_prime_UTR | 35.0% | |
| AATGGGGAGGAATAAGAAAA+AGG | - | chr7_2:76867246-76867265 | None:intergenic | 35.0% | |
| AATTGAATTGATGAATGGGG+AGG | - | chr7_2:76867259-76867278 | None:intergenic | 35.0% | |
| ! | AGTTTTCTTCCTCATTCGAA+GGG | - | chr7_2:76867338-76867357 | None:intergenic | 35.0% |
| GAATGAGGAAGAAAACTCAT+CGG | + | chr7_2:76867341-76867360 | Msa1078000:five_prime_UTR | 35.0% | |
| TCATCGGTGAATGAACAAAA+TGG | + | chr7_2:76867357-76867376 | Msa1078000:exon | 35.0% | |
| CATCGGTGAATGAACAAAAT+GGG | + | chr7_2:76867358-76867377 | Msa1078000:exon | 35.0% | |
| ATCGGTGAATGAACAAAATG+GGG | + | chr7_2:76867359-76867378 | Msa1078000:exon | 35.0% | |
| ! | TAGCAGTGTTGAATCTAATG+CGG | + | chr7_2:76867390-76867409 | Msa1078000:CDS | 35.0% |
| ATCGTTGGTGTATCAGTATT+AGG | + | chr7_2:76867439-76867458 | Msa1078000:CDS | 35.0% | |
| ! | TTCTTTTTCCACAACCCAAA+TGG | - | chr7_2:76867484-76867503 | None:intergenic | 35.0% |
| !! | TTTGGGTTGTGGAAAAAGAA+TGG | + | chr7_2:76867484-76867503 | Msa1078000:CDS | 35.0% |
| GGAAAAAGAATGGTAGAAGA+AGG | + | chr7_2:76867494-76867513 | Msa1078000:CDS | 35.0% | |
| CCATCCATATAAACACGAAT+CGG | - | chr7_2:76867529-76867548 | None:intergenic | 35.0% | |
| ! | CTTGGTGATCAATTGATTGT+TGG | + | chr7_2:76867604-76867623 | Msa1078000:CDS | 35.0% |
| ACGTTTATTCCCTTCGAATG+AGG | + | chr7_2:76867326-76867345 | Msa1078000:five_prime_UTR | 40.0% | |
| ! | GAGTTTTCTTCCTCATTCGA+AGG | - | chr7_2:76867339-76867358 | None:intergenic | 40.0% |
| TCGGTGAATGAACAAAATGG+GGG | + | chr7_2:76867360-76867379 | Msa1078000:exon | 40.0% | |
| TATTCATCTTCTGGGCCATT+TGG | + | chr7_2:76867466-76867485 | Msa1078000:CDS | 40.0% | |
| ATTCATCTTCTGGGCCATTT+GGG | + | chr7_2:76867467-76867486 | Msa1078000:CDS | 40.0% | |
| ! | CCGATTCGTGTTTATATGGA+TGG | + | chr7_2:76867526-76867545 | Msa1078000:CDS | 40.0% |
| CTTTCATGTAAAGGGGTAAC+AGG | - | chr7_2:76867670-76867689 | None:intergenic | 40.0% | |
| CTGTTACCCCTTTACATGAA+AGG | + | chr7_2:76867668-76867687 | Msa1078000:CDS | 40.0% | |
| CGGTGAATGAACAAAATGGG+GGG | + | chr7_2:76867361-76867380 | Msa1078000:exon | 45.0% | |
| ! | CAGTGTTGAATCTAATGCGG+CGG | + | chr7_2:76867393-76867412 | Msa1078000:CDS | 45.0% |
| TTGGTCGGAGTTATCATCGT+TGG | + | chr7_2:76867424-76867443 | Msa1078000:CDS | 45.0% | |
| TCATGTAAAGGGGTAACAGG+AGG | - | chr7_2:76867667-76867686 | None:intergenic | 45.0% | |
| AATCTAATGCGGCGGTGAAG+TGG | + | chr7_2:76867401-76867420 | Msa1078000:CDS | 50.0% | |
| TAATGCGGCGGTGAAGTGGT+TGG | + | chr7_2:76867405-76867424 | Msa1078000:CDS | 55.0% | |
| !! | CTTCTGGGCCATTTGGGTTG+TGG | + | chr7_2:76867473-76867492 | Msa1078000:CDS | 55.0% |
| TTGCGTCAAGCTCGTGCTCT+TGG | + | chr7_2:76867586-76867605 | Msa1078000:CDS | 55.0% | |
| GCGGCGGTGAAGTGGTTGGT+CGG | + | chr7_2:76867409-76867428 | Msa1078000:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_2 | gene | 76867132 | 76867818 | 76867132 | ID=Msa1078000;Name=Msa1078000 |
| chr7_2 | mRNA | 76867132 | 76867818 | 76867132 | ID=Msa1078000-mRNA-1;Parent=Msa1078000;Name=Msa1078000-mRNA-1;_AED=0.12;_eAED=0.12;_QI=235|0|0.5|0.5|1|1|2|0|115 |
| chr7_2 | exon | 76867132 | 76867689 | 76867132 | ID=Msa1078000-mRNA-1:exon:18686;Parent=Msa1078000-mRNA-1 |
| chr7_2 | exon | 76867794 | 76867818 | 76867794 | ID=Msa1078000-mRNA-1:exon:18687;Parent=Msa1078000-mRNA-1 |
| chr7_2 | five_prime_UTR | 76867132 | 76867366 | 76867132 | ID=Msa1078000-mRNA-1:five_prime_utr;Parent=Msa1078000-mRNA-1 |
| chr7_2 | CDS | 76867367 | 76867689 | 76867367 | ID=Msa1078000-mRNA-1:cds;Parent=Msa1078000-mRNA-1 |
| chr7_2 | CDS | 76867794 | 76867818 | 76867794 | ID=Msa1078000-mRNA-1:cds;Parent=Msa1078000-mRNA-1 |
| Gene Sequence |
| Protein sequence |
