Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1463470 | AES91364.1 | 88.462 | 78 | 8 | 1 | 1 | 78 | 1 | 77 | 8.66e-37 | 140 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1463470 | sp|Q9SGE9|SYFB_ARATH | 75.000 | 72 | 17 | 1 | 1 | 72 | 1 | 71 | 1.92e-27 | 106 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1463470 | A0A2P2MTQ4 | 82.051 | 78 | 14 | 0 | 1 | 78 | 1 | 78 | 1.17e-39 | 138 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0003220 | Msa1463470 | 0.841080 | 5.900848e-58 | -8.615850e-47 |
| Msa0003270 | Msa1463470 | 0.830414 | 2.976283e-55 | -8.615850e-47 |
| Msa0010770 | Msa1463470 | 0.827932 | 1.190546e-54 | -8.615850e-47 |
| Msa0041690 | Msa1463470 | 0.814060 | 1.867601e-51 | -8.615850e-47 |
| Msa0047250 | Msa1463470 | 0.805515 | 1.286367e-49 | -8.615850e-47 |
| Msa0068430 | Msa1463470 | 0.808406 | 3.146890e-50 | -8.615850e-47 |
| Msa0096580 | Msa1463470 | 0.801579 | 8.426194e-49 | -8.615850e-47 |
| Msa0101180 | Msa1463470 | 0.800504 | 1.397350e-48 | -8.615850e-47 |
| Msa0108870 | Msa1463470 | 0.802423 | 5.648971e-49 | -8.615850e-47 |
| Msa1344850 | Msa1463470 | 0.805408 | 1.354571e-49 | -8.615850e-47 |
| Msa1393800 | Msa1463470 | 0.852353 | 4.881770e-61 | -8.615850e-47 |
| Msa1397940 | Msa1463470 | 0.811456 | 6.937516e-51 | -8.615850e-47 |
| Msa1398860 | Msa1463470 | 0.809991 | 1.439274e-50 | -8.615850e-47 |
| Msa1426910 | Msa1463470 | 0.803453 | 3.460413e-49 | -8.615850e-47 |
| Msa1438450 | Msa1463470 | 0.841772 | 3.879159e-58 | -8.615850e-47 |
| Msa1458030 | Msa1463470 | 0.804610 | 1.989144e-49 | -8.615850e-47 |
| Msa1463470 | Msa1464290 | 0.809622 | 1.727752e-50 | -8.615850e-47 |
| Msa1463470 | Msa1466590 | 0.823981 | 1.033482e-53 | -8.615850e-47 |
| Msa0628500 | Msa1463470 | 0.806777 | 6.976453e-50 | -8.615850e-47 |
| Msa0646330 | Msa1463470 | 0.820800 | 5.664574e-53 | -8.615850e-47 |
| Msa0659990 | Msa1463470 | 0.811060 | 8.454878e-51 | -8.615850e-47 |
| Msa0679100 | Msa1463470 | 0.807604 | 4.661172e-50 | -8.615850e-47 |
| Msa0687230 | Msa1463470 | 0.826275 | 2.966180e-54 | -8.615850e-47 |
| Msa0687450 | Msa1463470 | 0.808196 | 3.487429e-50 | -8.615850e-47 |
| Msa0734960 | Msa1463470 | 0.834320 | 3.208295e-56 | -8.615850e-47 |
| Msa0743530 | Msa1463470 | 0.800803 | 1.214481e-48 | -8.615850e-47 |
| Msa0749400 | Msa1463470 | 0.823319 | 1.476491e-53 | -8.615850e-47 |
| Msa0756930 | Msa1463470 | 0.801892 | 7.264345e-49 | -8.615850e-47 |
| Msa0788120 | Msa1463470 | 0.805626 | 1.219179e-49 | -8.615850e-47 |
| Msa0795660 | Msa1463470 | 0.812291 | 4.564112e-51 | -8.615850e-47 |
| Msa0126230 | Msa1463470 | 0.809290 | 2.035941e-50 | -8.615850e-47 |
| Msa0127040 | Msa1463470 | 0.820348 | 7.193070e-53 | -8.615850e-47 |
| Msa0127850 | Msa1463470 | 0.800438 | 1.441544e-48 | -8.615850e-47 |
| Msa0144780 | Msa1463470 | 0.809035 | 2.308906e-50 | -8.615850e-47 |
| Msa0174000 | Msa1463470 | 0.812104 | 5.013733e-51 | -8.615850e-47 |
| Msa0179000 | Msa1463470 | 0.809090 | 2.247261e-50 | -8.615850e-47 |
| Msa0183420 | Msa1463470 | 0.801157 | 1.027750e-48 | -8.615850e-47 |
| Msa0202830 | Msa1463470 | 0.800157 | 1.644303e-48 | -8.615850e-47 |
| Msa0210260 | Msa1463470 | 0.800372 | 1.486227e-48 | -8.615850e-47 |
| Msa0215360 | Msa1463470 | 0.802241 | 6.159616e-49 | -8.615850e-47 |
| Msa1227320 | Msa1463470 | 0.813364 | 2.657766e-51 | -8.615850e-47 |
| Msa1227690 | Msa1463470 | 0.803638 | 3.167752e-49 | -8.615850e-47 |
| Msa1259640 | Msa1463470 | 0.807171 | 5.757164e-50 | -8.615850e-47 |
| Msa1294840 | Msa1463470 | 0.820340 | 7.221980e-53 | -8.615850e-47 |
| Msa1296300 | Msa1463470 | 0.831978 | 1.228725e-55 | -8.615850e-47 |
| Msa1299360 | Msa1463470 | 0.812466 | 4.179323e-51 | -8.615850e-47 |
| Msa1327490 | Msa1463470 | 0.801525 | 8.643169e-49 | -8.615850e-47 |
| Msa1329450 | Msa1463470 | 0.815312 | 9.858852e-52 | -8.615850e-47 |
| Msa1329790 | Msa1463470 | 0.802480 | 5.500435e-49 | -8.615850e-47 |
| Msa1338250 | Msa1463470 | 0.811422 | 7.055576e-51 | -8.615850e-47 |
| Msa0381000 | Msa1463470 | 0.826079 | 3.303354e-54 | -8.615850e-47 |
| Msa0381080 | Msa1463470 | 0.807624 | 4.614505e-50 | -8.615850e-47 |
| Msa0384670 | Msa1463470 | 0.808603 | 2.855328e-50 | -8.615850e-47 |
| Msa0403190 | Msa1463470 | 0.815582 | 8.585911e-52 | -8.615850e-47 |
| Msa0431410 | Msa1463470 | 0.805616 | 1.225072e-49 | -8.615850e-47 |
| Msa0432070 | Msa1463470 | 0.807070 | 6.047222e-50 | -8.615850e-47 |
| Msa0434570 | Msa1463470 | 0.803676 | 3.112225e-49 | -8.615850e-47 |
| Msa0434770 | Msa1463470 | 0.809151 | 2.180368e-50 | -8.615850e-47 |
| Msa0446900 | Msa1463470 | 0.825641 | 4.196062e-54 | -8.615850e-47 |
| Msa0449820 | Msa1463470 | 0.801137 | 1.037682e-48 | -8.615850e-47 |
| Msa0474700 | Msa1463470 | 0.813238 | 2.832590e-51 | -8.615850e-47 |
| Msa0478580 | Msa1463470 | 0.805004 | 1.645760e-49 | -8.615850e-47 |
| Msa0980940 | Msa1463470 | 0.809023 | 2.322019e-50 | -8.615850e-47 |
| Msa0981430 | Msa1463470 | 0.813141 | 2.974905e-51 | -8.615850e-47 |
| Msa1003440 | Msa1463470 | 0.823800 | 1.139329e-53 | -8.615850e-47 |
| Msa1036740 | Msa1463470 | 0.831970 | 1.234043e-55 | -8.615850e-47 |
| Msa1051600 | Msa1463470 | 0.808760 | 2.643097e-50 | -8.615850e-47 |
| Msa1070390 | Msa1463470 | 0.814561 | 1.446934e-51 | -8.615850e-47 |
| Msa1078000 | Msa1463470 | 0.804514 | 2.082413e-49 | -8.615850e-47 |
| Msa1083880 | Msa1463470 | 0.819210 | 1.309143e-52 | -8.615850e-47 |
| Msa1098890 | Msa1463470 | 0.800702 | 1.273410e-48 | -8.615850e-47 |
| Msa0322590 | Msa1463470 | 0.848372 | 6.392547e-60 | -8.615850e-47 |
| Msa1113590 | Msa1463470 | 0.835600 | 1.526705e-56 | -8.615850e-47 |
| Msa1122780 | Msa1463470 | 0.817415 | 3.337154e-52 | -8.615850e-47 |
| Msa1127430 | Msa1463470 | 0.806753 | 7.059243e-50 | -8.615850e-47 |
| Msa1127540 | Msa1463470 | 0.817923 | 2.562695e-52 | -8.615850e-47 |
| Msa1148580 | Msa1463470 | 0.807561 | 4.759326e-50 | -8.615850e-47 |
| Msa1163870 | Msa1463470 | 0.803243 | 3.826029e-49 | -8.615850e-47 |
| Msa1178050 | Msa1463470 | 0.800417 | 1.455245e-48 | -8.615850e-47 |
| Msa1181170 | Msa1463470 | -0.817497 | 3.198730e-52 | -8.615850e-47 |
| Msa1192850 | Msa1463470 | 0.800272 | 1.558204e-48 | -8.615850e-47 |
| Msa1199710 | Msa1463470 | 0.813936 | 1.988789e-51 | -8.615850e-47 |
| Msa1201810 | Msa1463470 | 0.814321 | 1.635348e-51 | -8.615850e-47 |
| Msa1201830 | Msa1463470 | 0.820272 | 7.486256e-53 | -8.615850e-47 |
| Msa1205480 | Msa1463470 | 0.804606 | 1.992806e-49 | -8.615850e-47 |
| Msa0518960 | Msa1463470 | 0.815099 | 1.099650e-51 | -8.615850e-47 |
| Msa0537770 | Msa1463470 | 0.805436 | 1.336375e-49 | -8.615850e-47 |
| Msa0578090 | Msa1463470 | 0.803401 | 3.548446e-49 | -8.615850e-47 |
| Msa0869010 | Msa1463470 | 0.820599 | 6.299540e-53 | -8.615850e-47 |
| Msa0898660 | Msa1463470 | 0.802275 | 6.059828e-49 | -8.615850e-47 |
| Msa0976330 | Msa1463470 | 0.805269 | 1.448470e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1463470 | MtrunA17_Chr4g0061991 | 88.462 | 78 | 8 | 1 | 1 | 78 | 1 | 77 | 1.07e-40 | 140 |
| Msa1463470 | MtrunA17_Chr5g0434271 | 93.590 | 78 | 5 | 0 | 1 | 78 | 1 | 78 | 3.22e-38 | 126 |
| Msa1463470 | MtrunA17_Chr8g0376591 | 93.590 | 78 | 5 | 0 | 1 | 78 | 1 | 78 | 2.93e-36 | 127 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1463470 | AT1G72550.2 | 75.000 | 72 | 17 | 1 | 1 | 72 | 1 | 71 | 1.94e-28 | 106 |
| Msa1463470 | AT1G72550.1 | 75.000 | 72 | 17 | 1 | 1 | 72 | 1 | 71 | 1.96e-28 | 106 |
Find 22 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCTAAACTCAACCCAAATTT+TGG | 0.066996 | tig0043319:-8540 | Msa1463470:intergenic |
| CGCACTTCTTAAACAACTTA+TGG | 0.119920 | tig0043319:-8588 | Msa1463470:intergenic |
| AGCTAACTTTGTTTCATTTC+AGG | 0.181501 | tig0043319:+8560 | Msa1463470:five_prime_UTR |
| GAAGACCTATGCTTCAGTTT+TGG | 0.314391 | tig0043319:+8797 | Msa1463470:CDS |
| AGCATGCCTACCGTGAGTTT+AGG | 0.345299 | tig0043319:+8643 | Msa1463470:exon |
| GCATGCCTACCGTGAGTTTA+GGG | 0.375297 | tig0043319:+8644 | Msa1463470:exon |
| GCACTTCTTAAACAACTTAT+GGG | 0.386235 | tig0043319:-8587 | Msa1463470:intergenic |
| AAATTGAAATTCCTGCTAAT+AGG | 0.389907 | tig0043319:+9009 | Msa1463470:CDS |
| ATTGCAGGGACTATAATGAT+AGG | 0.397822 | tig0043319:-9040 | Msa1463470:intergenic |
| TTGCAGGGACTATAATGATA+GGG | 0.511070 | tig0043319:-9039 | Msa1463470:intergenic |
| AGGGGTGGGTACCTATTAGC+AGG | 0.533127 | tig0043319:-9020 | Msa1463470:intergenic |
| GCCTACCGTGAGTTTAGGGC+GGG | 0.550193 | tig0043319:+8648 | Msa1463470:CDS |
| CAATTCCAAAACTGAAGCAT+AGG | 0.560611 | tig0043319:-8802 | Msa1463470:intergenic |
| TGCTGTTCTTCGCAAAATCG+CGG | 0.567136 | tig0043319:-8624 | Msa1463470:intergenic |
| AGAACAAGAAGAAGCTGATG+AGG | 0.587439 | tig0043319:+8968 | Msa1463470:CDS |
| GATCGACTGTTTGCAGCTCT+AGG | 0.594041 | tig0043319:+8670 | Msa1463470:CDS |
| TGCAGGGACTATAATGATAG+GGG | 0.627939 | tig0043319:-9038 | Msa1463470:intergenic |
| TCGATCCCGCCCTAAACTCA+CGG | 0.638719 | tig0043319:-8653 | Msa1463470:intergenic |
| AGGGACTATAATGATAGGGG+TGG | 0.640922 | tig0043319:-9035 | Msa1463470:intergenic |
| TGCCTACCGTGAGTTTAGGG+CGG | 0.643150 | tig0043319:+8647 | Msa1463470:CDS |
| GGGACTATAATGATAGGGGT+GGG | 0.646255 | tig0043319:-9034 | Msa1463460:intergenic |
| TCCCGCCCTAAACTCACGGT+AGG | 0.675464 | tig0043319:-8649 | Msa1463460:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTATTATGATGATAATTAGT+TGG | - | tig0043319:8872-8891 | Msa1463470:intergenic | 15.0% |
| !! | AAAAAAACTAACCCAAAATT+TGG | + | tig0043319:8528-8547 | Msa1463470:five_prime_UTR | 20.0% |
| !! | AAAAAACTAACCCAAAATTT+GGG | + | tig0043319:8529-8548 | Msa1463470:five_prime_UTR | 20.0% |
| ! | TAGATAGAAGATTGATTGAA+AGG | - | tig0043319:8727-8746 | Msa1463470:intergenic | 25.0% |
| ! | AAATTGAAATTCCTGCTAAT+AGG | + | tig0043319:9009-9028 | Msa1463470:CDS | 25.0% |
| ! | CTAAACTCAACCCAAATTTT+GGG | - | tig0043319:8542-8561 | Msa1463470:intergenic | 30.0% |
| ! | AGCTAACTTTGTTTCATTTC+AGG | + | tig0043319:8560-8579 | Msa1463470:five_prime_UTR | 30.0% |
| GCACTTCTTAAACAACTTAT+GGG | - | tig0043319:8590-8609 | Msa1463470:intergenic | 30.0% | |
| AGATTGATTGAAAGGAGAAA+TGG | - | tig0043319:8719-8738 | Msa1463470:intergenic | 30.0% | |
| GTTGGAGTTGGAAATAAAAA+GGG | - | tig0043319:8854-8873 | Msa1463470:intergenic | 30.0% | |
| AGTTGGAGTTGGAAATAAAA+AGG | - | tig0043319:8855-8874 | Msa1463470:intergenic | 30.0% | |
| TGATGATAATTAGTTGGAGT+TGG | - | tig0043319:8866-8885 | Msa1463470:intergenic | 30.0% | |
| ! | GCTAAACTCAACCCAAATTT+TGG | - | tig0043319:8543-8562 | Msa1463470:intergenic | 35.0% |
| CGCACTTCTTAAACAACTTA+TGG | - | tig0043319:8591-8610 | Msa1463470:intergenic | 35.0% | |
| TGATTGAAAGGAGAAATGGA+AGG | - | tig0043319:8715-8734 | Msa1463470:intergenic | 35.0% | |
| TCTTCAAATTCATCTTGTGC+TGG | - | tig0043319:8782-8801 | Msa1463470:intergenic | 35.0% | |
| CAATTCCAAAACTGAAGCAT+AGG | - | tig0043319:8805-8824 | Msa1463470:intergenic | 35.0% | |
| TTCATCTTGTGCTGGAGAAA+TGG | - | tig0043319:8774-8793 | Msa1463470:intergenic | 40.0% | |
| ! | GAAGACCTATGCTTCAGTTT+TGG | + | tig0043319:8797-8816 | Msa1463470:CDS | 40.0% |
| AGAACAAGAAGAAGCTGATG+AGG | + | tig0043319:8968-8987 | Msa1463470:CDS | 40.0% | |
| TGCTGTTCTTCGCAAAATCG+CGG | - | tig0043319:8627-8646 | Msa1463470:intergenic | 45.0% | |
| AGCATGCCTACCGTGAGTTT+AGG | + | tig0043319:8643-8662 | Msa1463470:exon | 50.0% | |
| GCATGCCTACCGTGAGTTTA+GGG | + | tig0043319:8644-8663 | Msa1463470:exon | 50.0% | |
| GATCGACTGTTTGCAGCTCT+AGG | + | tig0043319:8670-8689 | Msa1463470:CDS | 50.0% | |
| TGCCTACCGTGAGTTTAGGG+CGG | + | tig0043319:8647-8666 | Msa1463470:CDS | 55.0% | |
| TCGATCCCGCCCTAAACTCA+CGG | - | tig0043319:8656-8675 | Msa1463470:intergenic | 55.0% | |
| AGGGGTGGGTACCTATTAGC+AGG | - | tig0043319:9023-9042 | Msa1463470:intergenic | 55.0% | |
| ! | GCCTACCGTGAGTTTAGGGC+GGG | + | tig0043319:8648-8667 | Msa1463470:CDS | 60.0% |
| TCCCGCCCTAAACTCACGGT+AGG | - | tig0043319:8652-8671 | Msa1463470:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0043319 | gene | 8518 | 9052 | 8518 | ID=Msa1463470;Name=Msa1463470 |
| tig0043319 | mRNA | 8518 | 9052 | 8518 | ID=Msa1463470-mRNA-1;Parent=Msa1463470;Name=Msa1463470-mRNA-1;_AED=0.11;_eAED=0.11;_QI=128|1|0.66|1|1|1|3|0|78 |
| tig0043319 | exon | 8518 | 8703 | 8518 | ID=Msa1463470-mRNA-1:exon:24058;Parent=Msa1463470-mRNA-1 |
| tig0043319 | exon | 8783 | 8838 | 8783 | ID=Msa1463470-mRNA-1:exon:24059;Parent=Msa1463470-mRNA-1 |
| tig0043319 | exon | 8930 | 9052 | 8930 | ID=Msa1463470-mRNA-1:exon:24060;Parent=Msa1463470-mRNA-1 |
| tig0043319 | five_prime_UTR | 8518 | 8645 | 8518 | ID=Msa1463470-mRNA-1:five_prime_utr;Parent=Msa1463470-mRNA-1 |
| tig0043319 | CDS | 8646 | 8703 | 8646 | ID=Msa1463470-mRNA-1:cds;Parent=Msa1463470-mRNA-1 |
| tig0043319 | CDS | 8783 | 8838 | 8783 | ID=Msa1463470-mRNA-1:cds;Parent=Msa1463470-mRNA-1 |
| tig0043319 | CDS | 8930 | 9052 | 8930 | ID=Msa1463470-mRNA-1:cds;Parent=Msa1463470-mRNA-1 |
| Gene Sequence |
| Protein sequence |
