AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0850150


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0850150 MtrunA17_Chr4g0072411 100.000 103 0 0 1 103 59 161 5.39e-69 203
Msa0850150 MtrunA17_Chr5g0425951 100.000 103 0 0 1 103 1 103 2.26e-68 199
Msa0850150 MtrunA17_Chr4g0030431 100.000 103 0 0 1 103 1 103 2.26e-68 199
Msa0850150 MtrunA17_Chr3g0100121 100.000 103 0 0 1 103 1 103 2.26e-68 199
Msa0850150 MtrunA17_Chr3g0113681 100.000 103 0 0 1 103 1 103 2.26e-68 199
Msa0850150 MtrunA17_Chr2g0327641 100.000 103 0 0 1 103 1 103 2.26e-68 199
Msa0850150 MtrunA17_Chr3g0103351 100.000 103 0 0 1 103 1 103 2.26e-68 199
Msa0850150 MtrunA17_Chr8g0352791 100.000 103 0 0 1 103 1 103 2.26e-68 199
Msa0850150 MtrunA17_Chr3g0103821 100.000 103 0 0 1 103 1 103 2.26e-68 199
Msa0850150 MtrunA17_Chr3g0104031 100.000 103 0 0 1 103 1 103 2.26e-68 199
Msa0850150 MtrunA17_Chr7g0225731 100.000 103 0 0 1 103 1 103 2.26e-68 199
Msa0850150 MtrunA17_Chr3g0103801 100.000 103 0 0 1 103 1 103 2.26e-68 199
Msa0850150 MtrunA17_Chr3g0103881 100.000 103 0 0 1 103 1 103 2.26e-68 199
Msa0850150 MtrunA17_Chr7g0262791 100.000 103 0 0 1 103 1 103 2.26e-68 199
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0850150 AT3G53730.1 100.000 103 0 0 1 103 1 103 3.11e-68 199
Msa0850150 AT1G07820.2 100.000 103 0 0 1 103 1 103 3.11e-68 199
Msa0850150 AT1G07820.1 100.000 103 0 0 1 103 1 103 3.11e-68 199
Msa0850150 AT5G59690.1 100.000 103 0 0 1 103 1 103 3.11e-68 199
Msa0850150 AT3G46320.1 100.000 103 0 0 1 103 1 103 3.11e-68 199
Msa0850150 AT2G28740.1 100.000 103 0 0 1 103 1 103 3.11e-68 199
Msa0850150 AT1G07660.1 100.000 103 0 0 1 103 1 103 3.11e-68 199
Msa0850150 AT3G45930.1 100.000 103 0 0 1 103 1 103 3.11e-68 199
Msa0850150 AT5G59970.1 100.000 103 0 0 1 103 1 103 3.11e-68 199
Msa0850150 AT5G59970.2 100.000 103 0 0 1 103 1 103 3.11e-68 199
Msa0850150 AT1G07660.2 83.495 103 0 1 1 103 1 86 4.52e-51 155

Find 26 sgRNAs with CRISPR-Local

Find 28 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAAGGACCCTTTATGGTTT+TGG 0.243182 5_4:+38755960 Msa0850150:CDS
AGACAAGGAAGGACCCTTTA+TGG 0.291085 5_4:+38755954 Msa0850150:CDS
AGGAAAAGGAGGAAAGGGTC+TGG 0.345687 5_4:+38755689 Msa0850150:CDS
GGAAAAGGAGGAAAGGGTCT+GGG 0.428161 5_4:+38755690 Msa0850150:CDS
GTTCTTCGAGATAACATTCA+AGG 0.428481 5_4:+38755741 Msa0850150:CDS
AAGACGACGAATCGCAGGTT+TGG 0.443598 5_4:-38755769 None:intergenic
GGAGGAAAGGGTCTGGGAAA+GGG 0.463171 5_4:+38755696 Msa0850150:CDS
AGGAGGAAAGGGTCTGGGAA+AGG 0.465557 5_4:+38755695 Msa0850150:CDS
GGAAAGGGTCTGGGAAAGGG+TGG 0.472991 5_4:+38755699 Msa0850150:CDS
ATCTTGCAATCAACAATGTC+AGG 0.473641 5_4:+38755663 None:intergenic
TCGCAAGACGGTTACTGCTA+TGG 0.479581 5_4:+38755911 Msa0850150:CDS
ACAATGTCAGGAAGAGGAAA+AGG 0.493795 5_4:+38755675 None:intergenic
GGAAGAGGAAAAGGAGGAAA+GGG 0.523720 5_4:+38755684 Msa0850150:CDS
CTGCGATTCGTCGTCTTGCA+AGG 0.537712 5_4:+38755775 Msa0850150:CDS
CAATCAACAATGTCAGGAAG+AGG 0.542220 5_4:+38755669 None:intergenic
AGGAAGAGGAAAAGGAGGAA+AGG 0.557567 5_4:+38755683 Msa0850150:CDS
CTTGCAAGACGACGAATCGC+AGG 0.572639 5_4:-38755774 None:intergenic
CGTCGTCTTGCAAGGCGTGG+TGG 0.615268 5_4:+38755783 Msa0850150:CDS
ATTCGTCGTCTTGCAAGGCG+TGG 0.619919 5_4:+38755780 Msa0850150:CDS
ATGTCAGGAAGAGGAAAAGG+AGG 0.630961 5_4:+38755678 Msa0850150:CDS
GTTTATGCACTCAAGAGACA+AGG 0.649593 5_4:+38755939 Msa0850150:CDS
TCGTGATGCTGTAACCTACA+CGG 0.656428 5_4:+38755878 Msa0850150:CDS
GGTGGTGTGAAGCGTATCAG+TGG 0.661880 5_4:+38755801 Msa0850150:CDS
GGAACACGCTCGTCGCAAGA+CGG 0.672406 5_4:+38755899 Msa0850150:CDS
ATGCACTCAAGAGACAAGGA+AGG 0.704360 5_4:+38755943 Msa0850150:CDS
CTGATCTATGAAGAGACTCG+TGG 0.706611 5_4:+38755825 Msa0850150:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
GTTCTTCGAGATAACATTCA+AGG + chr5_4:38755741-38755760 Msa0850150:CDS 35.0%
GTTTATGCACTCAAGAGACA+AGG + chr5_4:38755939-38755958 Msa0850150:CDS 40.0%
! GAAGGACCCTTTATGGTTTT+GGG + chr5_4:38755961-38755980 Msa0850150:CDS 40.0%
!! AAGGACCCTTTATGGTTTTG+GGG + chr5_4:38755962-38755981 Msa0850150:CDS 40.0%
CTAACCCCCAAAACCATAAA+GGG - chr5_4:38755970-38755989 None:intergenic 40.0%
ATGTCAGGAAGAGGAAAAGG+AGG + chr5_4:38755678-38755697 Msa0850150:CDS 45.0%
AGGAAGAGGAAAAGGAGGAA+AGG + chr5_4:38755683-38755702 Msa0850150:CDS 45.0%
GGAAGAGGAAAAGGAGGAAA+GGG + chr5_4:38755684-38755703 Msa0850150:CDS 45.0%
CTGATCTATGAAGAGACTCG+TGG + chr5_4:38755825-38755844 Msa0850150:CDS 45.0%
TCGTGATGCTGTAACCTACA+CGG + chr5_4:38755878-38755897 Msa0850150:CDS 45.0%
ATGCACTCAAGAGACAAGGA+AGG + chr5_4:38755943-38755962 Msa0850150:CDS 45.0%
AGACAAGGAAGGACCCTTTA+TGG + chr5_4:38755954-38755973 Msa0850150:CDS 45.0%
! GGAAGGACCCTTTATGGTTT+TGG + chr5_4:38755960-38755979 Msa0850150:CDS 45.0%
!!! AGGACCCTTTATGGTTTTGG+GGG + chr5_4:38755963-38755982 Msa0850150:CDS 45.0%
AGGAAAAGGAGGAAAGGGTC+TGG + chr5_4:38755689-38755708 Msa0850150:CDS 50.0%
GGAAAAGGAGGAAAGGGTCT+GGG + chr5_4:38755690-38755709 Msa0850150:CDS 50.0%
AAGACGACGAATCGCAGGTT+TGG - chr5_4:38755772-38755791 None:intergenic 50.0%
TCGCAAGACGGTTACTGCTA+TGG + chr5_4:38755911-38755930 Msa0850150:CDS 50.0%
AGGAGGAAAGGGTCTGGGAA+AGG + chr5_4:38755695-38755714 Msa0850150:CDS 55.0%
GGAGGAAAGGGTCTGGGAAA+GGG + chr5_4:38755696-38755715 Msa0850150:CDS 55.0%
CTTGCAAGACGACGAATCGC+AGG - chr5_4:38755777-38755796 None:intergenic 55.0%
CTGCGATTCGTCGTCTTGCA+AGG + chr5_4:38755775-38755794 Msa0850150:CDS 55.0%
ATTCGTCGTCTTGCAAGGCG+TGG + chr5_4:38755780-38755799 Msa0850150:CDS 55.0%
GGTGGTGTGAAGCGTATCAG+TGG + chr5_4:38755801-38755820 Msa0850150:CDS 55.0%
GGAAAGGGTCTGGGAAAGGG+TGG + chr5_4:38755699-38755718 Msa0850150:CDS 60.0%
GGAACACGCTCGTCGCAAGA+CGG + chr5_4:38755899-38755918 Msa0850150:CDS 60.0%
CGTCGTCTTGCAAGGCGTGG+TGG + chr5_4:38755783-38755802 Msa0850150:CDS 65.0%
GCGACGAGCGTGTTCCGTGT+AGG - chr5_4:38755895-38755914 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 38755678 38755989 38755678 ID=Msa0850150;Name=Msa0850150
chr5_4 mRNA 38755678 38755989 38755678 ID=Msa0850150-mRNA-1;Parent=Msa0850150;Name=Msa0850150-mRNA-1;_AED=0.25;_eAED=0.25;_QI=0|-1|0|1|-1|1|1|0|103
chr5_4 exon 38755678 38755989 38755678 ID=Msa0850150-mRNA-1:exon:12412;Parent=Msa0850150-mRNA-1
chr5_4 CDS 38755678 38755989 38755678 ID=Msa0850150-mRNA-1:cds;Parent=Msa0850150-mRNA-1
Gene Sequence

>Msa0850150

ATGTCAGGAAGAGGAAAAGGAGGAAAGGGTCTGGGAAAGGGTGGCGCAAAGCGTCATCGCAAAGTTCTTCGAGATAACATTCAAGGCATCACCAAACCTGCGATTCGTCGTCTTGCAAGGCGTGGTGGTGTGAAGCGTATCAGTGGTCTGATCTATGAAGAGACTCGTGGTGTTCTCAAGATATTTCTCGAGAATGTGATTCGTGATGCTGTAACCTACACGGAACACGCTCGTCGCAAGACGGTTACTGCTATGGATGTTGTTTATGCACTCAAGAGACAAGGAAGGACCCTTTATGGTTTTGGGGGTTAG

Protein sequence

>Msa0850150

MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVVYALKRQGRTLYGFGG*