AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1393800


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1393800 MtrunA17_Chr1g0213491 98.450 129 2 0 3 131 10 138 7.29e-85 253
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1393800 AT4G10430.4 53.968 126 55 1 6 131 2 124 2.11e-35 124
Msa1393800 AT4G10430.6 53.968 126 55 1 6 131 2 124 2.11e-35 124
Msa1393800 AT4G10430.9 53.968 126 55 1 6 131 2 124 2.11e-35 124
Msa1393800 AT4G10430.5 53.968 126 55 1 6 131 2 124 2.11e-35 124
Msa1393800 AT4G10430.7 53.968 126 55 1 6 131 2 124 2.87e-35 124
Msa1393800 AT4G10430.1 53.968 126 55 1 6 131 2 124 3.91e-35 125
Msa1393800 AT4G10430.3 53.968 126 55 1 6 131 2 124 3.91e-35 125
Msa1393800 AT1G33230.1 57.391 115 46 1 17 131 14 125 8.11e-34 121
Msa1393800 AT1G33230.2 75.000 52 13 0 80 131 81 132 3.00e-23 93.6
Msa1393800 AT4G10430.8 68.750 48 15 0 84 131 1 48 1.96e-17 76.3
Msa1393800 AT4G10430.2 68.750 48 15 0 84 131 1 48 2.65e-17 76.3

Find 31 sgRNAs with CRISPR-Local

Find 116 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGAGAGGCACGAACATTAAT+TGG 0.209868 tig0021759:+453 None:intergenic
AGAAGAAGCTAAAGAGATTC+AGG 0.250609 tig0021759:-892 Msa1393800:CDS
AAGAGGAATACAACAGTTAC+AGG 0.348052 tig0021759:-407 Msa1393800:CDS
AAGAAAAGAAAAGAGTTGAT+TGG 0.376177 tig0021759:-1002 Msa1393800:five_prime_UTR
TCAACCATCTTTAGCTGATA+AGG 0.392804 tig0021759:-739 Msa1393800:intron
GATGCTTCTTACCTCCTTCC+TGG 0.396954 tig0021759:-603 Msa1393800:CDS
GAAGAGAAGAAGAGGTTCAA+TGG 0.405539 tig0021759:-955 Msa1393800:exon
GCCAGATGCATCATCGTTGA+TGG 0.420149 tig0021759:-627 Msa1393800:CDS
TGATGGCGAATTCGAGAATT+CGG 0.437774 tig0021759:+168 None:intergenic
CTCCTTCCTGGACATGCTCA+AGG 0.460262 tig0021759:-591 Msa1393800:intron
GCCATCAACGATGATGCATC+TGG 0.467300 tig0021759:+626 None:intergenic
GAAGAAGAATCAAGGGAACT+TGG 0.513739 tig0021759:-191 Msa1393800:CDS
CATCAGAGGAAGAAGAATCA+AGG 0.518685 tig0021759:-199 Msa1393800:CDS
TCGAATTCGCCATCAGTGAC+AGG 0.523051 tig0021759:-160 Msa1393800:CDS
AGGTTCAATGGAAGAATCAA+AGG 0.539036 tig0021759:-943 Msa1393800:exon
CGTACCTTATCAGCTAAAGA+TGG 0.544500 tig0021759:+735 None:intergenic
TGAGAAGAGAAGAGAAGAAG+AGG 0.550186 tig0021759:-963 Msa1393800:five_prime_UTR
AAGAAACCTTGAGCATGTCC+AGG 0.557316 tig0021759:+585 None:intergenic
TCGCAAGGATGTTCAGCTCA+AGG 0.568890 tig0021759:-433 Msa1393800:CDS
ATCAGAGGAAGAAGAATCAA+GGG 0.570978 tig0021759:-198 Msa1393800:CDS
TAATGTTCGTGCCTCTCGCA+AGG 0.587007 tig0021759:-448 Msa1393800:CDS
TAATGTCTTTGAGATGTGAA+CGG 0.599008 tig0021759:+860 None:intergenic
CTTGAGCATGTCCAGGAAGG+AGG 0.603322 tig0021759:+592 None:intergenic
AACCTTGAGCATGTCCAGGA+AGG 0.613692 tig0021759:+589 None:intergenic
ACAAGGGCACGCTGACGAAG+TGG 0.615895 tig0021759:+825 None:intergenic
ACTACATGCCTAGGCATCAG+AGG 0.621344 tig0021759:-213 Msa1393800:intron
GTGACCATGCCTGTCACTGA+TGG 0.638463 tig0021759:+151 None:intergenic
TTCGCCATCAGTGACAGGCA+TGG 0.647646 tig0021759:-155 None:intergenic
AGAGGAATACAACAGTTACA+GGG 0.648843 tig0021759:-406 Msa1393800:intron
GAGCTGAACATCCTTGCGAG+AGG 0.657636 tig0021759:+437 None:intergenic
TGTTCAGCTCAAGGTCAAAG+AGG 0.660588 tig0021759:-424 Msa1393800:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAAATAAAGAAATTGAAAA+TGG + tig0021759:500-519 None:intergenic 10.0%
!!! TTTTCAATTTCTTTATTTAA+TGG - tig0021759:500-519 Msa1393800:intron 10.0%
!!! TTTTTTTTTTTTTTACTGTT+AGG - tig0021759:850-869 Msa1393800:CDS 10.0%
!! TTAAATAAAGAAATTGAAAA+TGG + tig0021759:500-519 None:intergenic 10.0%
!!! TTTTCAATTTCTTTATTTAA+TGG - tig0021759:500-519 Msa1393800:intron 10.0%
!!! TTTTTTTTTTTTTTACTGTT+AGG - tig0021759:850-869 Msa1393800:CDS 10.0%
!!! ACTTAAATTTTAGGTAATTA+TGG + tig0021759:893-912 None:intergenic 15.0%
!! GTTATTATTATTAATCAAAC+TGG - tig0021759:911-930 Msa1393800:CDS 15.0%
!!! ACTTAAATTTTAGGTAATTA+TGG + tig0021759:893-912 None:intergenic 15.0%
!! GTTATTATTATTAATCAAAC+TGG - tig0021759:911-930 Msa1393800:CDS 15.0%
!! AAAAAAAGATTAACATGCAA+GGG + tig0021759:831-850 None:intergenic 20.0%
!! AAAAAAAAGATTAACATGCA+AGG + tig0021759:832-851 None:intergenic 20.0%
!!! TTTTTTTTTACTGTTAGGAA+AGG - tig0021759:855-874 Msa1393800:CDS 20.0%
!! TTATTATTAATCAAACTGGA+AGG - tig0021759:915-934 Msa1393800:CDS 20.0%
!!! TTGTTATTGACTTTTTAACT+AGG + tig0021759:957-976 None:intergenic 20.0%
!! AAAAAAAGATTAACATGCAA+GGG + tig0021759:831-850 None:intergenic 20.0%
!! AAAAAAAAGATTAACATGCA+AGG + tig0021759:832-851 None:intergenic 20.0%
!!! TTTTTTTTTACTGTTAGGAA+AGG - tig0021759:855-874 Msa1393800:CDS 20.0%
!! TTATTATTAATCAAACTGGA+AGG - tig0021759:915-934 Msa1393800:CDS 20.0%
!!! TTGTTATTGACTTTTTAACT+AGG + tig0021759:957-976 None:intergenic 20.0%
!! TAAAAAAGTGATGAGTTGAA+TGG - tig0021759:163-182 Msa1393800:CDS 25.0%
!! AAAAAAGTGATGAGTTGAAT+GGG - tig0021759:164-183 Msa1393800:CDS 25.0%
!! AAGAAAAGAAAAGAGTTGAT+TGG - tig0021759:187-206 Msa1393800:CDS 25.0%
!! TAAAAAAGTGATGAGTTGAA+TGG - tig0021759:163-182 Msa1393800:CDS 25.0%
!! AAAAAAGTGATGAGTTGAAT+GGG - tig0021759:164-183 Msa1393800:CDS 25.0%
!! AAGAAAAGAAAAGAGTTGAT+TGG - tig0021759:187-206 Msa1393800:CDS 25.0%
!! TAATGTCTTTGAGATGTGAA+CGG + tig0021759:332-351 None:intergenic 30.0%
!!! GATTTTAGAATCGAGAACAA+GGG + tig0021759:383-402 None:intergenic 30.0%
!!! TGATTTTAGAATCGAGAACA+AGG + tig0021759:384-403 None:intergenic 30.0%
GAAAATGGAGAATAAAGAGT+TGG + tig0021759:485-504 None:intergenic 30.0%
CATCTAACTGCGATAATAAA+CGG + tig0021759:528-547 None:intergenic 30.0%
!! TAAGTTTCTGAGGATGTTTT+TGG - tig0021759:711-730 Msa1393800:intron 30.0%
!! AAGTTTCTGAGGATGTTTTT+GGG - tig0021759:712-731 Msa1393800:intron 30.0%
!! TAATGTCTTTGAGATGTGAA+CGG + tig0021759:332-351 None:intergenic 30.0%
!!! GATTTTAGAATCGAGAACAA+GGG + tig0021759:383-402 None:intergenic 30.0%
!!! TGATTTTAGAATCGAGAACA+AGG + tig0021759:384-403 None:intergenic 30.0%
GAAAATGGAGAATAAAGAGT+TGG + tig0021759:485-504 None:intergenic 30.0%
CATCTAACTGCGATAATAAA+CGG + tig0021759:528-547 None:intergenic 30.0%
!! TAAGTTTCTGAGGATGTTTT+TGG - tig0021759:711-730 Msa1393800:intron 30.0%
!! AAGTTTCTGAGGATGTTTTT+GGG - tig0021759:712-731 Msa1393800:intron 30.0%
AGGTTCAATGGAAGAATCAA+AGG - tig0021759:246-265 Msa1393800:intron 35.0%
AGAAGAAGCTAAAGAGATTC+AGG - tig0021759:297-316 Msa1393800:intron 35.0%
TCAACCATCTTTAGCTGATA+AGG - tig0021759:450-469 Msa1393800:CDS 35.0%
AAGAGGAATACAACAGTTAC+AGG - tig0021759:782-801 Msa1393800:CDS 35.0%
AGAGGAATACAACAGTTACA+GGG - tig0021759:783-802 Msa1393800:CDS 35.0%
AAAAGATTAACATGCAAGGG+AGG + tig0021759:828-847 None:intergenic 35.0%
TCAATAACAACTACATGCCT+AGG - tig0021759:967-986 Msa1393800:five_prime_UTR 35.0%
ATCAGAGGAAGAAGAATCAA+GGG - tig0021759:991-1010 Msa1393800:five_prime_UTR 35.0%
AGGTTCAATGGAAGAATCAA+AGG - tig0021759:246-265 Msa1393800:intron 35.0%
AGAAGAAGCTAAAGAGATTC+AGG - tig0021759:297-316 Msa1393800:intron 35.0%
TCAACCATCTTTAGCTGATA+AGG - tig0021759:450-469 Msa1393800:CDS 35.0%
AAGAGGAATACAACAGTTAC+AGG - tig0021759:782-801 Msa1393800:CDS 35.0%
AGAGGAATACAACAGTTACA+GGG - tig0021759:783-802 Msa1393800:CDS 35.0%
AAAAGATTAACATGCAAGGG+AGG + tig0021759:828-847 None:intergenic 35.0%
TCAATAACAACTACATGCCT+AGG - tig0021759:967-986 Msa1393800:five_prime_UTR 35.0%
ATCAGAGGAAGAAGAATCAA+GGG - tig0021759:991-1010 Msa1393800:five_prime_UTR 35.0%
TGAGAAGAGAAGAGAAGAAG+AGG - tig0021759:226-245 Msa1393800:intron 40.0%
GAAGAGAAGAAGAGGTTCAA+TGG - tig0021759:234-253 Msa1393800:intron 40.0%
CGTACCTTATCAGCTAAAGA+TGG + tig0021759:457-476 None:intergenic 40.0%
TCTTTAGCTGATAAGGTACG+AGG - tig0021759:457-476 Msa1393800:CDS 40.0%
! TTCATTCATTTTCGTCCCCA+CGG - tig0021759:680-699 Msa1393800:intron 40.0%
AAACTTACCTAAAAGCCGTG+GGG + tig0021759:698-717 None:intergenic 40.0%
GAAACTTACCTAAAAGCCGT+GGG + tig0021759:699-718 None:intergenic 40.0%
AGAAACTTACCTAAAAGCCG+TGG + tig0021759:700-719 None:intergenic 40.0%
!! GGCTTTTAGGTAAGTTTCTG+AGG - tig0021759:701-720 Msa1393800:intron 40.0%
CATCAGAGGAAGAAGAATCA+AGG - tig0021759:990-1009 Msa1393800:five_prime_UTR 40.0%
GAAGAAGAATCAAGGGAACT+TGG - tig0021759:998-1017 Msa1393800:five_prime_UTR 40.0%
! TGATGGCGAATTCGAGAATT+CGG + tig0021759:1024-1043 None:intergenic 40.0%
TGAGAAGAGAAGAGAAGAAG+AGG - tig0021759:226-245 Msa1393800:intron 40.0%
GAAGAGAAGAAGAGGTTCAA+TGG - tig0021759:234-253 Msa1393800:intron 40.0%
CGTACCTTATCAGCTAAAGA+TGG + tig0021759:457-476 None:intergenic 40.0%
TCTTTAGCTGATAAGGTACG+AGG - tig0021759:457-476 Msa1393800:CDS 40.0%
! TTCATTCATTTTCGTCCCCA+CGG - tig0021759:680-699 Msa1393800:intron 40.0%
AAACTTACCTAAAAGCCGTG+GGG + tig0021759:698-717 None:intergenic 40.0%
GAAACTTACCTAAAAGCCGT+GGG + tig0021759:699-718 None:intergenic 40.0%
AGAAACTTACCTAAAAGCCG+TGG + tig0021759:700-719 None:intergenic 40.0%
!! GGCTTTTAGGTAAGTTTCTG+AGG - tig0021759:701-720 Msa1393800:intron 40.0%
CATCAGAGGAAGAAGAATCA+AGG - tig0021759:990-1009 Msa1393800:five_prime_UTR 40.0%
GAAGAAGAATCAAGGGAACT+TGG - tig0021759:998-1017 Msa1393800:five_prime_UTR 40.0%
! TGATGGCGAATTCGAGAATT+CGG + tig0021759:1024-1043 None:intergenic 40.0%
AAGAAACCTTGAGCATGTCC+AGG + tig0021759:607-626 None:intergenic 45.0%
! CGAGAGGCACGAACATTAAT+TGG + tig0021759:739-758 None:intergenic 45.0%
TGTTCAGCTCAAGGTCAAAG+AGG - tig0021759:765-784 Msa1393800:CDS 45.0%
TTCTTCTTCCTCTGATGCCT+AGG + tig0021759:987-1006 None:intergenic 45.0%
AAGAAACCTTGAGCATGTCC+AGG + tig0021759:607-626 None:intergenic 45.0%
! CGAGAGGCACGAACATTAAT+TGG + tig0021759:739-758 None:intergenic 45.0%
TGTTCAGCTCAAGGTCAAAG+AGG - tig0021759:765-784 Msa1393800:CDS 45.0%
TTCTTCTTCCTCTGATGCCT+AGG + tig0021759:987-1006 None:intergenic 45.0%
!!! ATAATAATAACTTAAATTTT+AGG + tig0021759:902-921 None:intergenic 5.0%
!!! ATAATAATAACTTAAATTTT+AGG + tig0021759:902-921 None:intergenic 5.0%
GCCAGATGCATCATCGTTGA+TGG - tig0021759:562-581 Msa1393800:intron 50.0%
GCCATCAACGATGATGCATC+TGG + tig0021759:566-585 None:intergenic 50.0%
GATGCTTCTTACCTCCTTCC+TGG - tig0021759:586-605 Msa1393800:intron 50.0%
AACCTTGAGCATGTCCAGGA+AGG + tig0021759:603-622 None:intergenic 50.0%
! TTTTCGTCCCCACGGCTTTT+AGG - tig0021759:688-707 Msa1393800:intron 50.0%
TAATGTTCGTGCCTCTCGCA+AGG - tig0021759:741-760 Msa1393800:CDS 50.0%
TCGCAAGGATGTTCAGCTCA+AGG - tig0021759:756-775 Msa1393800:CDS 50.0%
ACTACATGCCTAGGCATCAG+AGG - tig0021759:976-995 Msa1393800:five_prime_UTR 50.0%
TCGAATTCGCCATCAGTGAC+AGG - tig0021759:1029-1048 Msa1393800:five_prime_UTR 50.0%
GCCAGATGCATCATCGTTGA+TGG - tig0021759:562-581 Msa1393800:intron 50.0%
GCCATCAACGATGATGCATC+TGG + tig0021759:566-585 None:intergenic 50.0%
GATGCTTCTTACCTCCTTCC+TGG - tig0021759:586-605 Msa1393800:intron 50.0%
AACCTTGAGCATGTCCAGGA+AGG + tig0021759:603-622 None:intergenic 50.0%
! TTTTCGTCCCCACGGCTTTT+AGG - tig0021759:688-707 Msa1393800:intron 50.0%
TAATGTTCGTGCCTCTCGCA+AGG - tig0021759:741-760 Msa1393800:CDS 50.0%
TCGCAAGGATGTTCAGCTCA+AGG - tig0021759:756-775 Msa1393800:CDS 50.0%
ACTACATGCCTAGGCATCAG+AGG - tig0021759:976-995 Msa1393800:five_prime_UTR 50.0%
TCGAATTCGCCATCAGTGAC+AGG - tig0021759:1029-1048 Msa1393800:five_prime_UTR 50.0%
CTTGAGCATGTCCAGGAAGG+AGG + tig0021759:600-619 None:intergenic 55.0%
CTCCTTCCTGGACATGCTCA+AGG - tig0021759:598-617 Msa1393800:CDS 55.0%
GAGCTGAACATCCTTGCGAG+AGG + tig0021759:755-774 None:intergenic 55.0%
CTTGAGCATGTCCAGGAAGG+AGG + tig0021759:600-619 None:intergenic 55.0%
CTCCTTCCTGGACATGCTCA+AGG - tig0021759:598-617 Msa1393800:CDS 55.0%
GAGCTGAACATCCTTGCGAG+AGG + tig0021759:755-774 None:intergenic 55.0%
! ACAAGGGCACGCTGACGAAG+TGG + tig0021759:367-386 None:intergenic 60.0%
! ACAAGGGCACGCTGACGAAG+TGG + tig0021759:367-386 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
tig0021759 gene 157 1054 157 ID=Msa1393800;Name=Msa1393800
tig0021759 mRNA 157 1054 157 ID=Msa1393800-mRNA-1;Parent=Msa1393800;Name=Msa1393800-mRNA-1;_AED=0.15;_eAED=0.15;_QI=96|0.33|0.25|0.5|1|1|4|0|153
tig0021759 exon 157 222 157 ID=Msa1393800-mRNA-1:exon:14031;Parent=Msa1393800-mRNA-1
tig0021759 exon 407 501 407 ID=Msa1393800-mRNA-1:exon:14030;Parent=Msa1393800-mRNA-1
tig0021759 exon 592 670 592 ID=Msa1393800-mRNA-1:exon:14029;Parent=Msa1393800-mRNA-1
tig0021759 exon 740 1054 740 ID=Msa1393800-mRNA-1:exon:14028;Parent=Msa1393800-mRNA-1
tig0021759 five_prime_UTR 959 1054 959 ID=Msa1393800-mRNA-1:five_prime_utr;Parent=Msa1393800-mRNA-1
tig0021759 CDS 740 958 740 ID=Msa1393800-mRNA-1:cds;Parent=Msa1393800-mRNA-1
tig0021759 CDS 592 670 592 ID=Msa1393800-mRNA-1:cds;Parent=Msa1393800-mRNA-1
tig0021759 CDS 407 501 407 ID=Msa1393800-mRNA-1:cds;Parent=Msa1393800-mRNA-1
tig0021759 CDS 157 222 157 ID=Msa1393800-mRNA-1:cds;Parent=Msa1393800-mRNA-1
Gene Sequence

>Msa1393800

ATGGAAGAATCAAAGGAGATAGAAGAATCAGTTGTAAGCGTATTAGAAGAAGCTAAAGAGATTCAGGATTCAGTTTCCGTTCACATCTCAAAGACATTAAGCGATGAACAACCACTTCGTCAGCGTGCCCTTGTTCTCGATTCTAAAATCATTTCTCTTCGTTCTTCTCTTTATTCTCTTCTTTCTAATAAACTCATTCAACCATCTTTAGCTGATAAGTTAGATGAAGATTTGCAAAGAGCCAGATGCATCATCGTTGATGGCGATGCTTCTTACCTCCTTCCTGGACATGCTCAAGGTAAGTTTCTGAGGATGTTTTTGGGTCCAATTAATGTTCGTGCCTCTCGCAAGGATGTTCAGCTCAAGGTCAAAGAGGAATACAACAGTTACAGGGCATCAGAGGAAGAAGAATCAAGGGAACTTGGCCGAATTCTCGAATTCGCCATCAGTGACAGGCAT

Protein sequence

>Msa1393800

MEESKEIEESVVSVLEEAKEIQDSVSVHISKTLSDEQPLRQRALVLDSKIISLRSSLYSLLSNKLIQPSLADKLDEDLQRARCIIVDGDASYLLPGHAQGKFLRMFLGPINVRASRKDVQLKVKEEYNSYRASEEEESRELGRILEFAISDRH