AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1140150

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1140150 MtrunA17_Chr7g0221371 96.970 231 7 0 1 231 1 231 4.82e-169 464
Msa1140150 MtrunA17_Chr4g0020161 94.220 173 10 0 59 231 1 173 5.25e-122 343
Msa1140150 MtrunA17_Chr7g0270171 73.057 193 47 2 44 231 1 193 6.67e-102 293
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1140150 AT5G23710.1 37.209 215 128 3 21 231 19 230 2.57e-47 156

Find 44 sgRNAs with CRISPR-Local

Find 282 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGATGACAAGTACTGGATA+TGG 0.326445 7_4:-11545785 Msa1140150:CDS
CGGCGAAGTGTGACTCCAAA+TGG 0.352806 7_4:+11547439 None:intergenic
ATGAAACGTTTGCAAGAATC+TGG 0.362818 7_4:-11546277 Msa1140150:CDS
GATGTTTGGAGTGGGTTAAA+AGG 0.362955 7_4:-11545889 Msa1140150:CDS
AAGCTGTGTATATAACAAAT+AGG 0.381521 7_4:+11547494 None:intergenic
GGCACTTATAAACAAGAGTA+TGG 0.381632 7_4:-11546098 Msa1140150:CDS
GAGCCTTCCAAGGATATAAC+TGG 0.383383 7_4:-11546016 Msa1140150:CDS
TGAAAATAGACACATGTTGT+TGG 0.391859 7_4:+11545623 None:intergenic
TTCCACATGTGATGGATGTT+TGG 0.402495 7_4:-11545903 Msa1140150:CDS
GTGCTTGCAATGCATTTCAT+TGG 0.426796 7_4:-11545933 Msa1140150:CDS
GAAATGGTTGGATTTCTGAA+TGG 0.445730 7_4:-11545600 Msa1140150:exon
TAGAGTATACGCTCTTCATC+TGG 0.446970 7_4:+11546217 None:intergenic
TGTTTATTAAGCGCAGCAAT+AGG 0.447212 7_4:+11546127 None:intergenic
ATGCCCACCAGTTATATCCT+TGG 0.459091 7_4:+11546009 None:intergenic
TGGAGTGGGTTAAAAGGAGT+GGG 0.480413 7_4:-11545883 Msa1140150:CDS
AGATGACAAGTACTGGATAT+GGG 0.485810 7_4:-11545784 Msa1140150:CDS
AAATGGTTGGATTTCTGAAT+GGG 0.494317 7_4:-11545599 Msa1140150:exon
CAAAGGTATAAAGATCTACA+TGG 0.502338 7_4:-11546050 Msa1140150:CDS
ATAATGCAGATGACAAGTAC+TGG 0.506988 7_4:-11545791 Msa1140150:CDS
GGCGAAGTGTGACTCCAAAT+GGG 0.521369 7_4:+11547440 None:intergenic
ATACTTAGCAAGGAAAACTC+AGG 0.536886 7_4:-11546187 Msa1140150:CDS
AGCGACCACCGATTCTCGCC+GGG 0.543848 7_4:+11547401 None:intergenic
TATAAACAAGAGTATGGTAA+AGG 0.546896 7_4:-11546092 Msa1140150:CDS
CATTTGGAGTCACACTTCGC+CGG 0.552787 7_4:-11547438 Msa1140150:five_prime_UTR
TTGGAGTGGGTTAAAAGGAG+TGG 0.562912 7_4:-11545884 Msa1140150:CDS
AACAGAATTTGAGCCTTCCA+AGG 0.568058 7_4:-11546026 Msa1140150:CDS
TATTAAGCGCAGCAATAGGA+AGG 0.568531 7_4:+11546131 None:intergenic
GAGAGAGTTTCCACATGTGA+TGG 0.576485 7_4:-11545911 Msa1140150:CDS
TTGTTACATATGCAAAAGCA+AGG 0.588438 7_4:-11545732 Msa1140150:CDS
GTCATCACAACTCAAGTCAC+GGG 0.589333 7_4:-11545860 Msa1140150:CDS
TGCGCTTAATAAACACATGA+AGG 0.598707 7_4:-11546119 Msa1140150:CDS
CTATGACCTTATACTTAGCA+AGG 0.601401 7_4:-11546197 Msa1140150:CDS
CCACCAGTTATATCCTTGGA+AGG 0.602371 7_4:+11546013 None:intergenic
GGTCATCACAACTCAAGTCA+CGG 0.608686 7_4:-11545861 Msa1140150:CDS
CCTTCCAAGGATATAACTGG+TGG 0.618508 7_4:-11546013 Msa1140150:CDS
CTCCAAACATCCATCACATG+TGG 0.618959 7_4:+11545901 None:intergenic
TACTTAGCAAGGAAAACTCA+GGG 0.624253 7_4:-11546186 Msa1140150:CDS
GATGACAAGTACTGGATATG+GGG 0.631855 7_4:-11545783 Msa1140150:CDS
GGAGTGGGTTAAAAGGAGTG+GGG 0.639610 7_4:-11545882 Msa1140150:CDS
CATGTGATGGATGTTTGGAG+TGG 0.639686 7_4:-11545898 Msa1140150:CDS
ATGTGATGGATGTTTGGAGT+GGG 0.644389 7_4:-11545897 Msa1140150:CDS
TGTTACATATGCAAAAGCAA+GGG 0.651007 7_4:-11545731 Msa1140150:CDS
AAAGGTATAAAGATCTACAT+GGG 0.652631 7_4:-11546049 Msa1140150:CDS
CTTCCAAGGATATAACTGGT+GGG 0.673279 7_4:-11546012 Msa1140150:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAATTTATAACTTCATTTTA+AGG - chr7_4:11546656-11546675 Msa1140150:intron 10.0%
!! AATTAATTATATCATTCAAA+TGG + chr7_4:11546738-11546757 None:intergenic 10.0%
!!! TAATTTATAACTTCATTTTA+AGG - chr7_4:11546656-11546675 Msa1140150:intron 10.0%
!! AATTAATTATATCATTCAAA+TGG + chr7_4:11546738-11546757 None:intergenic 10.0%
!! TAAACAATTAATCAATTGTG+AGG + chr7_4:11545828-11545847 None:intergenic 20.0%
!! TTAGAAAATTGTTTATGCTA+TGG - chr7_4:11545857-11545876 Msa1140150:CDS 20.0%
!! AATAGAATAACTTACTTTCT+AGG + chr7_4:11545915-11545934 None:intergenic 20.0%
!! CAGATCTAAACATTATATAT+TGG + chr7_4:11546080-11546099 None:intergenic 20.0%
!!! ACTTTATCGCTTATAAATTT+TGG - chr7_4:11546274-11546293 Msa1140150:CDS 20.0%
!!! TAACATTTTCCAAACAAAAT+AGG + chr7_4:11546393-11546412 None:intergenic 20.0%
!!! CTTTATACCTTTGTTTTTAA+TGG + chr7_4:11547011-11547030 None:intergenic 20.0%
!! TAAACAATTAATCAATTGTG+AGG + chr7_4:11545828-11545847 None:intergenic 20.0%
!! TTAGAAAATTGTTTATGCTA+TGG - chr7_4:11545857-11545876 Msa1140150:CDS 20.0%
!! AATAGAATAACTTACTTTCT+AGG + chr7_4:11545915-11545934 None:intergenic 20.0%
!! CAGATCTAAACATTATATAT+TGG + chr7_4:11546080-11546099 None:intergenic 20.0%
!!! ACTTTATCGCTTATAAATTT+TGG - chr7_4:11546274-11546293 Msa1140150:CDS 20.0%
!!! TAACATTTTCCAAACAAAAT+AGG + chr7_4:11546393-11546412 None:intergenic 20.0%
!!! CTTTATACCTTTGTTTTTAA+TGG + chr7_4:11547011-11547030 None:intergenic 20.0%
! AAGCTGTGTATATAACAAAT+AGG + chr7_4:11545577-11545596 None:intergenic 25.0%
!! GTACTGTTGTTTATAGTTTA+TGG - chr7_4:11545879-11545898 Msa1140150:CDS 25.0%
! CTAAACATTATATATTGGTG+TGG + chr7_4:11546075-11546094 None:intergenic 25.0%
!!! CTTTTGGTGTTTTTATTCAA+AGG - chr7_4:11546242-11546261 Msa1140150:CDS 25.0%
!!! TGTTTTTATTCAAAGGTTTC+TGG - chr7_4:11546249-11546268 Msa1140150:CDS 25.0%
! GAACATGGAAAAAACAAAAT+GGG + chr7_4:11546579-11546598 None:intergenic 25.0%
!! TTTGACATTTTTCTCTTGTA+AGG - chr7_4:11546767-11546786 Msa1140150:intron 25.0%
!! TATAAACAAGAGTATGGTAA+AGG - chr7_4:11546976-11546995 Msa1140150:intron 25.0%
!!! ATACCTTTGTTTTTAATGGA+AGG + chr7_4:11547007-11547026 None:intergenic 25.0%
!! AAAGGTATAAAGATCTACAT+GGG - chr7_4:11547019-11547038 Msa1140150:intron 25.0%
! AAGCTGTGTATATAACAAAT+AGG + chr7_4:11545577-11545596 None:intergenic 25.0%
!! GTACTGTTGTTTATAGTTTA+TGG - chr7_4:11545879-11545898 Msa1140150:CDS 25.0%
! CTAAACATTATATATTGGTG+TGG + chr7_4:11546075-11546094 None:intergenic 25.0%
!!! CTTTTGGTGTTTTTATTCAA+AGG - chr7_4:11546242-11546261 Msa1140150:CDS 25.0%
!!! TGTTTTTATTCAAAGGTTTC+TGG - chr7_4:11546249-11546268 Msa1140150:CDS 25.0%
! GAACATGGAAAAAACAAAAT+GGG + chr7_4:11546579-11546598 None:intergenic 25.0%
!! TTTGACATTTTTCTCTTGTA+AGG - chr7_4:11546767-11546786 Msa1140150:intron 25.0%
!! TATAAACAAGAGTATGGTAA+AGG - chr7_4:11546976-11546995 Msa1140150:intron 25.0%
!!! ATACCTTTGTTTTTAATGGA+AGG + chr7_4:11547007-11547026 None:intergenic 25.0%
!! AAAGGTATAAAGATCTACAT+GGG - chr7_4:11547019-11547038 Msa1140150:intron 25.0%
AACATTATATATTGGTGTGG+CGG + chr7_4:11546072-11546091 None:intergenic 30.0%
!! TGCTATTTCATCATGATTCA+TGG - chr7_4:11546219-11546238 Msa1140150:CDS 30.0%
AGGAAACCAAATATAGCATA+AGG - chr7_4:11546438-11546457 Msa1140150:intron 30.0%
CAAAGTCCTTATGCTATATT+TGG + chr7_4:11546447-11546466 None:intergenic 30.0%
CAAATATAGCATAAGGACTT+TGG - chr7_4:11546445-11546464 Msa1140150:intron 30.0%
TCTGGTTAAGGAAACAAAAA+AGG + chr7_4:11546529-11546548 None:intergenic 30.0%
GGAACATGGAAAAAACAAAA+TGG + chr7_4:11546580-11546599 None:intergenic 30.0%
! AGTTTTCCTTGCTAAGTATA+AGG + chr7_4:11546880-11546899 None:intergenic 30.0%
CTTCCTTCCATTAAAAACAA+AGG - chr7_4:11547001-11547020 Msa1140150:intron 30.0%
! CAAAGGTATAAAGATCTACA+TGG - chr7_4:11547018-11547037 Msa1140150:intron 30.0%
! AGAAGAGATTTTGCAAACTT+TGG - chr7_4:11547240-11547259 Msa1140150:intron 30.0%
TTGTTACATATGCAAAAGCA+AGG - chr7_4:11547336-11547355 Msa1140150:intron 30.0%
TGTTACATATGCAAAAGCAA+GGG - chr7_4:11547337-11547356 Msa1140150:intron 30.0%
TGACAAGAAAAACATGGAAA+GGG + chr7_4:11547388-11547407 None:intergenic 30.0%
TTGACAAGAAAAACATGGAA+AGG + chr7_4:11547389-11547408 None:intergenic 30.0%
! TTCCATGTTTTTCTTGTCAA+CGG - chr7_4:11547389-11547408 Msa1140150:intron 30.0%
TGAAAATAGACACATGTTGT+TGG + chr7_4:11547448-11547467 None:intergenic 30.0%
! CATGTGTCTATTTTCAGAAA+TGG - chr7_4:11547452-11547471 Msa1140150:five_prime_UTR 30.0%
! TGTCTATTTTCAGAAATGGT+TGG - chr7_4:11547456-11547475 Msa1140150:five_prime_UTR 30.0%
AAATGGTTGGATTTCTGAAT+GGG - chr7_4:11547469-11547488 Msa1140150:five_prime_UTR 30.0%
AACATTATATATTGGTGTGG+CGG + chr7_4:11546072-11546091 None:intergenic 30.0%
!! TGCTATTTCATCATGATTCA+TGG - chr7_4:11546219-11546238 Msa1140150:CDS 30.0%
AGGAAACCAAATATAGCATA+AGG - chr7_4:11546438-11546457 Msa1140150:intron 30.0%
CAAAGTCCTTATGCTATATT+TGG + chr7_4:11546447-11546466 None:intergenic 30.0%
CAAATATAGCATAAGGACTT+TGG - chr7_4:11546445-11546464 Msa1140150:intron 30.0%
TCTGGTTAAGGAAACAAAAA+AGG + chr7_4:11546529-11546548 None:intergenic 30.0%
GGAACATGGAAAAAACAAAA+TGG + chr7_4:11546580-11546599 None:intergenic 30.0%
! AGTTTTCCTTGCTAAGTATA+AGG + chr7_4:11546880-11546899 None:intergenic 30.0%
CTTCCTTCCATTAAAAACAA+AGG - chr7_4:11547001-11547020 Msa1140150:intron 30.0%
! CAAAGGTATAAAGATCTACA+TGG - chr7_4:11547018-11547037 Msa1140150:intron 30.0%
! AGAAGAGATTTTGCAAACTT+TGG - chr7_4:11547240-11547259 Msa1140150:intron 30.0%
TTGTTACATATGCAAAAGCA+AGG - chr7_4:11547336-11547355 Msa1140150:intron 30.0%
TGTTACATATGCAAAAGCAA+GGG - chr7_4:11547337-11547356 Msa1140150:intron 30.0%
TGACAAGAAAAACATGGAAA+GGG + chr7_4:11547388-11547407 None:intergenic 30.0%
TTGACAAGAAAAACATGGAA+AGG + chr7_4:11547389-11547408 None:intergenic 30.0%
! TTCCATGTTTTTCTTGTCAA+CGG - chr7_4:11547389-11547408 Msa1140150:intron 30.0%
TGAAAATAGACACATGTTGT+TGG + chr7_4:11547448-11547467 None:intergenic 30.0%
! CATGTGTCTATTTTCAGAAA+TGG - chr7_4:11547452-11547471 Msa1140150:five_prime_UTR 30.0%
! TGTCTATTTTCAGAAATGGT+TGG - chr7_4:11547456-11547475 Msa1140150:five_prime_UTR 30.0%
AAATGGTTGGATTTCTGAAT+GGG - chr7_4:11547469-11547488 Msa1140150:five_prime_UTR 30.0%
!!! TGGGTTTTTGAGACAACAAA+GGG + chr7_4:11545612-11545631 None:intergenic 35.0%
!!! ATGGGTTTTTGAGACAACAA+AGG + chr7_4:11545613-11545632 None:intergenic 35.0%
TGTTGTCTCAAAAACCCATT+TGG - chr7_4:11545614-11545633 Msa1140150:CDS 35.0%
ATTTAGCGTGAACAAAGAGA+GGG + chr7_4:11545717-11545736 None:intergenic 35.0%
AATTTAGCGTGAACAAAGAG+AGG + chr7_4:11545718-11545737 None:intergenic 35.0%
! CTTCTTTGGTTTCTTTGTGA+TGG - chr7_4:11546002-11546021 Msa1140150:CDS 35.0%
AATTGATCAATGCCTTACCA+GGG + chr7_4:11546104-11546123 None:intergenic 35.0%
AAATTGATCAATGCCTTACC+AGG + chr7_4:11546105-11546124 None:intergenic 35.0%
! TCATCATGATTCATGGCTTT+TGG - chr7_4:11546226-11546245 Msa1140150:CDS 35.0%
! GTCATAGTGCCTATTTTGTT+TGG - chr7_4:11546381-11546400 Msa1140150:intron 35.0%
CCATGTTCCAAAATTTGAGA+CGG - chr7_4:11546591-11546610 Msa1140150:intron 35.0%
! TTCCAAAATTTGAGACGGAT+AGG - chr7_4:11546596-11546615 Msa1140150:intron 35.0%
! TCCAAAATTTGAGACGGATA+GGG - chr7_4:11546597-11546616 Msa1140150:intron 35.0%
ATGAAACGTTTGCAAGAATC+TGG - chr7_4:11546791-11546810 Msa1140150:intron 35.0%
!! TTTTTGTCGTTTCTGTTGAG+AGG + chr7_4:11546819-11546838 None:intergenic 35.0%
ACAGAAACGACAAAAACTGT+CGG - chr7_4:11546823-11546842 Msa1140150:intron 35.0%
CTATGACCTTATACTTAGCA+AGG - chr7_4:11546871-11546890 Msa1140150:intron 35.0%
ATACTTAGCAAGGAAAACTC+AGG - chr7_4:11546881-11546900 Msa1140150:intron 35.0%
TACTTAGCAAGGAAAACTCA+GGG - chr7_4:11546882-11546901 Msa1140150:intron 35.0%
TGTTTATTAAGCGCAGCAAT+AGG + chr7_4:11546944-11546963 None:intergenic 35.0%
TGCGCTTAATAAACACATGA+AGG - chr7_4:11546949-11546968 Msa1140150:intron 35.0%
GGCACTTATAAACAAGAGTA+TGG - chr7_4:11546970-11546989 Msa1140150:intron 35.0%
ATAATGCAGATGACAAGTAC+TGG - chr7_4:11547277-11547296 Msa1140150:intron 35.0%
!! AGATGACAAGTACTGGATAT+GGG - chr7_4:11547284-11547303 Msa1140150:intron 35.0%
! GATTTTGCATCTATTCCTGT+TGG - chr7_4:11547307-11547326 Msa1140150:intron 35.0%
! ATGTAACAAGTTTTGCCAAC+AGG + chr7_4:11547325-11547344 None:intergenic 35.0%
ATCCGTTGACAAGAAAAACA+TGG + chr7_4:11547394-11547413 None:intergenic 35.0%
GAAATGGTTGGATTTCTGAA+TGG - chr7_4:11547468-11547487 Msa1140150:five_prime_UTR 35.0%
!!! TGGGTTTTTGAGACAACAAA+GGG + chr7_4:11545612-11545631 None:intergenic 35.0%
!!! ATGGGTTTTTGAGACAACAA+AGG + chr7_4:11545613-11545632 None:intergenic 35.0%
TGTTGTCTCAAAAACCCATT+TGG - chr7_4:11545614-11545633 Msa1140150:CDS 35.0%
ATTTAGCGTGAACAAAGAGA+GGG + chr7_4:11545717-11545736 None:intergenic 35.0%
AATTTAGCGTGAACAAAGAG+AGG + chr7_4:11545718-11545737 None:intergenic 35.0%
! CTTCTTTGGTTTCTTTGTGA+TGG - chr7_4:11546002-11546021 Msa1140150:CDS 35.0%
AATTGATCAATGCCTTACCA+GGG + chr7_4:11546104-11546123 None:intergenic 35.0%
AAATTGATCAATGCCTTACC+AGG + chr7_4:11546105-11546124 None:intergenic 35.0%
! TCATCATGATTCATGGCTTT+TGG - chr7_4:11546226-11546245 Msa1140150:CDS 35.0%
! GTCATAGTGCCTATTTTGTT+TGG - chr7_4:11546381-11546400 Msa1140150:intron 35.0%
CCATGTTCCAAAATTTGAGA+CGG - chr7_4:11546591-11546610 Msa1140150:intron 35.0%
! TTCCAAAATTTGAGACGGAT+AGG - chr7_4:11546596-11546615 Msa1140150:intron 35.0%
! TCCAAAATTTGAGACGGATA+GGG - chr7_4:11546597-11546616 Msa1140150:intron 35.0%
ATGAAACGTTTGCAAGAATC+TGG - chr7_4:11546791-11546810 Msa1140150:intron 35.0%
!! TTTTTGTCGTTTCTGTTGAG+AGG + chr7_4:11546819-11546838 None:intergenic 35.0%
ACAGAAACGACAAAAACTGT+CGG - chr7_4:11546823-11546842 Msa1140150:intron 35.0%
CTATGACCTTATACTTAGCA+AGG - chr7_4:11546871-11546890 Msa1140150:intron 35.0%
ATACTTAGCAAGGAAAACTC+AGG - chr7_4:11546881-11546900 Msa1140150:intron 35.0%
TACTTAGCAAGGAAAACTCA+GGG - chr7_4:11546882-11546901 Msa1140150:intron 35.0%
TGTTTATTAAGCGCAGCAAT+AGG + chr7_4:11546944-11546963 None:intergenic 35.0%
TGCGCTTAATAAACACATGA+AGG - chr7_4:11546949-11546968 Msa1140150:intron 35.0%
GGCACTTATAAACAAGAGTA+TGG - chr7_4:11546970-11546989 Msa1140150:intron 35.0%
ATAATGCAGATGACAAGTAC+TGG - chr7_4:11547277-11547296 Msa1140150:intron 35.0%
!! AGATGACAAGTACTGGATAT+GGG - chr7_4:11547284-11547303 Msa1140150:intron 35.0%
! GATTTTGCATCTATTCCTGT+TGG - chr7_4:11547307-11547326 Msa1140150:intron 35.0%
! ATGTAACAAGTTTTGCCAAC+AGG + chr7_4:11547325-11547344 None:intergenic 35.0%
ATCCGTTGACAAGAAAAACA+TGG + chr7_4:11547394-11547413 None:intergenic 35.0%
GAAATGGTTGGATTTCTGAA+TGG - chr7_4:11547468-11547487 Msa1140150:five_prime_UTR 35.0%
GAGACAACAAAGGGGAAAAA+CGG + chr7_4:11545603-11545622 None:intergenic 40.0%
!!! GGGTTTTTGAGACAACAAAG+GGG + chr7_4:11545611-11545630 None:intergenic 40.0%
!! GAAATAGGTTCTGAATGTGC+AGG + chr7_4:11545969-11545988 None:intergenic 40.0%
AGAACCTATTTCCCTTCCTA+TGG - chr7_4:11545977-11545996 Msa1140150:CDS 40.0%
!! AGAAACCAAAGAAGCACCAT+AGG + chr7_4:11545996-11546015 None:intergenic 40.0%
ATAATGTTTAGATCTGCCCC+TGG - chr7_4:11546084-11546103 Msa1140150:CDS 40.0%
ATTGATCAATGCCTTACCAG+GGG + chr7_4:11546103-11546122 None:intergenic 40.0%
AAAATGTTAACTAGTGCCCC+CGG - chr7_4:11546404-11546423 Msa1140150:intron 40.0%
!!! CCGTCTCAAATTTTGGAACA+TGG + chr7_4:11546594-11546613 None:intergenic 40.0%
! ACCCTATCCGTCTCAAATTT+TGG + chr7_4:11546601-11546620 None:intergenic 40.0%
TAGAGTATACGCTCTTCATC+TGG + chr7_4:11546854-11546873 None:intergenic 40.0%
TATTAAGCGCAGCAATAGGA+AGG + chr7_4:11546940-11546959 None:intergenic 40.0%
AACAGAATTTGAGCCTTCCA+AGG - chr7_4:11547042-11547061 Msa1140150:intron 40.0%
CTTCCAAGGATATAACTGGT+GGG - chr7_4:11547056-11547075 Msa1140150:intron 40.0%
GTGCTTGCAATGCATTTCAT+TGG - chr7_4:11547135-11547154 Msa1140150:intron 40.0%
!! TTCCACATGTGATGGATGTT+TGG - chr7_4:11547165-11547184 Msa1140150:intron 40.0%
!! ATGTGATGGATGTTTGGAGT+GGG - chr7_4:11547171-11547190 Msa1140150:intron 40.0%
!! GATGTTTGGAGTGGGTTAAA+AGG - chr7_4:11547179-11547198 Msa1140150:intron 40.0%
!! CAGATGACAAGTACTGGATA+TGG - chr7_4:11547283-11547302 Msa1140150:intron 40.0%
!! GATGACAAGTACTGGATATG+GGG - chr7_4:11547285-11547304 Msa1140150:intron 40.0%
! ACGGATGAGCTTTTGTTCAA+CGG - chr7_4:11547408-11547427 Msa1140150:five_prime_UTR 40.0%
! ATGAGCTTTTGTTCAACGGA+TGG - chr7_4:11547412-11547431 Msa1140150:five_prime_UTR 40.0%
GAGACAACAAAGGGGAAAAA+CGG + chr7_4:11545603-11545622 None:intergenic 40.0%
!!! GGGTTTTTGAGACAACAAAG+GGG + chr7_4:11545611-11545630 None:intergenic 40.0%
!! GAAATAGGTTCTGAATGTGC+AGG + chr7_4:11545969-11545988 None:intergenic 40.0%
AGAACCTATTTCCCTTCCTA+TGG - chr7_4:11545977-11545996 Msa1140150:CDS 40.0%
!! AGAAACCAAAGAAGCACCAT+AGG + chr7_4:11545996-11546015 None:intergenic 40.0%
ATAATGTTTAGATCTGCCCC+TGG - chr7_4:11546084-11546103 Msa1140150:CDS 40.0%
ATTGATCAATGCCTTACCAG+GGG + chr7_4:11546103-11546122 None:intergenic 40.0%
AAAATGTTAACTAGTGCCCC+CGG - chr7_4:11546404-11546423 Msa1140150:intron 40.0%
!!! CCGTCTCAAATTTTGGAACA+TGG + chr7_4:11546594-11546613 None:intergenic 40.0%
! ACCCTATCCGTCTCAAATTT+TGG + chr7_4:11546601-11546620 None:intergenic 40.0%
TAGAGTATACGCTCTTCATC+TGG + chr7_4:11546854-11546873 None:intergenic 40.0%
TATTAAGCGCAGCAATAGGA+AGG + chr7_4:11546940-11546959 None:intergenic 40.0%
AACAGAATTTGAGCCTTCCA+AGG - chr7_4:11547042-11547061 Msa1140150:intron 40.0%
CTTCCAAGGATATAACTGGT+GGG - chr7_4:11547056-11547075 Msa1140150:intron 40.0%
GTGCTTGCAATGCATTTCAT+TGG - chr7_4:11547135-11547154 Msa1140150:intron 40.0%
!! TTCCACATGTGATGGATGTT+TGG - chr7_4:11547165-11547184 Msa1140150:intron 40.0%
!! ATGTGATGGATGTTTGGAGT+GGG - chr7_4:11547171-11547190 Msa1140150:intron 40.0%
!! GATGTTTGGAGTGGGTTAAA+AGG - chr7_4:11547179-11547198 Msa1140150:intron 40.0%
!! CAGATGACAAGTACTGGATA+TGG - chr7_4:11547283-11547302 Msa1140150:intron 40.0%
!! GATGACAAGTACTGGATATG+GGG - chr7_4:11547285-11547304 Msa1140150:intron 40.0%
! ACGGATGAGCTTTTGTTCAA+CGG - chr7_4:11547408-11547427 Msa1140150:five_prime_UTR 40.0%
! ATGAGCTTTTGTTCAACGGA+TGG - chr7_4:11547412-11547431 Msa1140150:five_prime_UTR 40.0%
! CCGGGAAAAATGGAGTTTTC+CGG + chr7_4:11545652-11545671 None:intergenic 45.0%
!! CCGGAAAACTCCATTTTTCC+CGG - chr7_4:11545649-11545668 Msa1140150:CDS 45.0%
AGCACCATAGGAAGGGAAAT+AGG + chr7_4:11545984-11546003 None:intergenic 45.0%
!! CCAAAGAAGCACCATAGGAA+GGG + chr7_4:11545991-11546010 None:intergenic 45.0%
!! ACCAAAGAAGCACCATAGGA+AGG + chr7_4:11545992-11546011 None:intergenic 45.0%
CTGTGGCTTCATGGTTACAA+TGG - chr7_4:11546185-11546204 Msa1140150:CDS 45.0%
AAATGTTAACTAGTGCCCCC+GGG - chr7_4:11546405-11546424 Msa1140150:intron 45.0%
GAGCCTTCCAAGGATATAAC+TGG - chr7_4:11547052-11547071 Msa1140150:intron 45.0%
CCACCAGTTATATCCTTGGA+AGG + chr7_4:11547058-11547077 None:intergenic 45.0%
CCTTCCAAGGATATAACTGG+TGG - chr7_4:11547055-11547074 Msa1140150:intron 45.0%
ATGCCCACCAGTTATATCCT+TGG + chr7_4:11547062-11547081 None:intergenic 45.0%
! GTGGGCATTTTTACAGCGAA+GGG - chr7_4:11547074-11547093 Msa1140150:intron 45.0%
GAGAGAGTTTCCACATGTGA+TGG - chr7_4:11547157-11547176 Msa1140150:intron 45.0%
CTCCAAACATCCATCACATG+TGG + chr7_4:11547170-11547189 None:intergenic 45.0%
!! CATGTGATGGATGTTTGGAG+TGG - chr7_4:11547170-11547189 Msa1140150:intron 45.0%
!! TTGGAGTGGGTTAAAAGGAG+TGG - chr7_4:11547184-11547203 Msa1140150:intron 45.0%
!! TGGAGTGGGTTAAAAGGAGT+GGG - chr7_4:11547185-11547204 Msa1140150:intron 45.0%
GGTCATCACAACTCAAGTCA+CGG - chr7_4:11547207-11547226 Msa1140150:intron 45.0%
GTCATCACAACTCAAGTCAC+GGG - chr7_4:11547208-11547227 Msa1140150:intron 45.0%
! CCGGGAAAAATGGAGTTTTC+CGG + chr7_4:11545652-11545671 None:intergenic 45.0%
!! CCGGAAAACTCCATTTTTCC+CGG - chr7_4:11545649-11545668 Msa1140150:CDS 45.0%
AGCACCATAGGAAGGGAAAT+AGG + chr7_4:11545984-11546003 None:intergenic 45.0%
!! CCAAAGAAGCACCATAGGAA+GGG + chr7_4:11545991-11546010 None:intergenic 45.0%
!! ACCAAAGAAGCACCATAGGA+AGG + chr7_4:11545992-11546011 None:intergenic 45.0%
CTGTGGCTTCATGGTTACAA+TGG - chr7_4:11546185-11546204 Msa1140150:CDS 45.0%
AAATGTTAACTAGTGCCCCC+GGG - chr7_4:11546405-11546424 Msa1140150:intron 45.0%
GAGCCTTCCAAGGATATAAC+TGG - chr7_4:11547052-11547071 Msa1140150:intron 45.0%
CCACCAGTTATATCCTTGGA+AGG + chr7_4:11547058-11547077 None:intergenic 45.0%
CCTTCCAAGGATATAACTGG+TGG - chr7_4:11547055-11547074 Msa1140150:intron 45.0%
ATGCCCACCAGTTATATCCT+TGG + chr7_4:11547062-11547081 None:intergenic 45.0%
! GTGGGCATTTTTACAGCGAA+GGG - chr7_4:11547074-11547093 Msa1140150:intron 45.0%
GAGAGAGTTTCCACATGTGA+TGG - chr7_4:11547157-11547176 Msa1140150:intron 45.0%
CTCCAAACATCCATCACATG+TGG + chr7_4:11547170-11547189 None:intergenic 45.0%
!! CATGTGATGGATGTTTGGAG+TGG - chr7_4:11547170-11547189 Msa1140150:intron 45.0%
!! TTGGAGTGGGTTAAAAGGAG+TGG - chr7_4:11547184-11547203 Msa1140150:intron 45.0%
!! TGGAGTGGGTTAAAAGGAGT+GGG - chr7_4:11547185-11547204 Msa1140150:intron 45.0%
GGTCATCACAACTCAAGTCA+CGG - chr7_4:11547207-11547226 Msa1140150:intron 45.0%
GTCATCACAACTCAAGTCAC+GGG - chr7_4:11547208-11547227 Msa1140150:intron 45.0%
GGCGAAGTGTGACTCCAAAT+GGG + chr7_4:11545631-11545650 None:intergenic 50.0%
! CATTTGGAGTCACACTTCGC+CGG - chr7_4:11545630-11545649 Msa1140150:CDS 50.0%
!! CCATTTTTCCCGGCGAGAAT+CGG - chr7_4:11545659-11545678 Msa1140150:CDS 50.0%
GCGTGAACAAAGAGAGGGAA+AGG + chr7_4:11545712-11545731 None:intergenic 50.0%
CCCTTCCTATGGTGCTTCTT+TGG - chr7_4:11545988-11546007 Msa1140150:CDS 50.0%
GTTTAGATCTGCCCCTGGTA+AGG - chr7_4:11546089-11546108 Msa1140150:CDS 50.0%
! GCACATGACTGTGACTAGTC+CGG - chr7_4:11546157-11546176 Msa1140150:CDS 50.0%
GTAACCATGAAGCCACAGTG+CGG + chr7_4:11546183-11546202 None:intergenic 50.0%
AATGTTAACTAGTGCCCCCG+GGG - chr7_4:11546406-11546425 Msa1140150:intron 50.0%
TTCCTTAACCAGACCAGTGC+CGG - chr7_4:11546536-11546555 Msa1140150:intron 50.0%
! GGTGGGCATTTTTACAGCGA+AGG - chr7_4:11547073-11547092 Msa1140150:intron 50.0%
!! GGAGTGGGTTAAAAGGAGTG+GGG - chr7_4:11547186-11547205 Msa1140150:intron 50.0%
GGCGAAGTGTGACTCCAAAT+GGG + chr7_4:11545631-11545650 None:intergenic 50.0%
! CATTTGGAGTCACACTTCGC+CGG - chr7_4:11545630-11545649 Msa1140150:CDS 50.0%
!! CCATTTTTCCCGGCGAGAAT+CGG - chr7_4:11545659-11545678 Msa1140150:CDS 50.0%
GCGTGAACAAAGAGAGGGAA+AGG + chr7_4:11545712-11545731 None:intergenic 50.0%
CCCTTCCTATGGTGCTTCTT+TGG - chr7_4:11545988-11546007 Msa1140150:CDS 50.0%
GTTTAGATCTGCCCCTGGTA+AGG - chr7_4:11546089-11546108 Msa1140150:CDS 50.0%
! GCACATGACTGTGACTAGTC+CGG - chr7_4:11546157-11546176 Msa1140150:CDS 50.0%
GTAACCATGAAGCCACAGTG+CGG + chr7_4:11546183-11546202 None:intergenic 50.0%
AATGTTAACTAGTGCCCCCG+GGG - chr7_4:11546406-11546425 Msa1140150:intron 50.0%
TTCCTTAACCAGACCAGTGC+CGG - chr7_4:11546536-11546555 Msa1140150:intron 50.0%
! GGTGGGCATTTTTACAGCGA+AGG - chr7_4:11547073-11547092 Msa1140150:intron 50.0%
!! GGAGTGGGTTAAAAGGAGTG+GGG - chr7_4:11547186-11547205 Msa1140150:intron 50.0%
CGGCGAAGTGTGACTCCAAA+TGG + chr7_4:11545632-11545651 None:intergenic 55.0%
CCGATTCTCGCCGGGAAAAA+TGG + chr7_4:11545662-11545681 None:intergenic 55.0%
! TTTTTCCCGGCGAGAATCGG+TGG - chr7_4:11545662-11545681 Msa1140150:CDS 55.0%
TTTCCTTAACCAGTGCCCCG+GGG + chr7_4:11546424-11546443 None:intergenic 55.0%
GTTTCCTTAACCAGTGCCCC+GGG + chr7_4:11546425-11546444 None:intergenic 55.0%
GGTTTCCTTAACCAGTGCCC+CGG + chr7_4:11546426-11546445 None:intergenic 55.0%
TCCTTAACCAGACCAGTGCC+GGG - chr7_4:11546537-11546556 Msa1140150:intron 55.0%
!! GGTCTTGTTAACCAGTGCCC+CGG + chr7_4:11546558-11546577 None:intergenic 55.0%
CGGCGAAGTGTGACTCCAAA+TGG + chr7_4:11545632-11545651 None:intergenic 55.0%
CCGATTCTCGCCGGGAAAAA+TGG + chr7_4:11545662-11545681 None:intergenic 55.0%
! TTTTTCCCGGCGAGAATCGG+TGG - chr7_4:11545662-11545681 Msa1140150:CDS 55.0%
TTTCCTTAACCAGTGCCCCG+GGG + chr7_4:11546424-11546443 None:intergenic 55.0%
GTTTCCTTAACCAGTGCCCC+GGG + chr7_4:11546425-11546444 None:intergenic 55.0%
GGTTTCCTTAACCAGTGCCC+CGG + chr7_4:11546426-11546445 None:intergenic 55.0%
TCCTTAACCAGACCAGTGCC+GGG - chr7_4:11546537-11546556 Msa1140150:intron 55.0%
!! GGTCTTGTTAACCAGTGCCC+CGG + chr7_4:11546558-11546577 None:intergenic 55.0%
!! AAGCGACCACCGATTCTCGC+CGG + chr7_4:11545671-11545690 None:intergenic 60.0%
TTCCTTAACCAGTGCCCCGG+GGG + chr7_4:11546423-11546442 None:intergenic 60.0%
CCTTAACCAGACCAGTGCCG+GGG - chr7_4:11546538-11546557 Msa1140150:intron 60.0%
!! AAGCGACCACCGATTCTCGC+CGG + chr7_4:11545671-11545690 None:intergenic 60.0%
TTCCTTAACCAGTGCCCCGG+GGG + chr7_4:11546423-11546442 None:intergenic 60.0%
CCTTAACCAGACCAGTGCCG+GGG - chr7_4:11546538-11546557 Msa1140150:intron 60.0%
!! AGCGACCACCGATTCTCGCC+GGG + chr7_4:11545670-11545689 None:intergenic 65.0%
! TGACTAGTCCGGCCGCACTG+TGG - chr7_4:11546168-11546187 Msa1140150:CDS 65.0%
CCATGAAGCCACAGTGCGGC+CGG + chr7_4:11546179-11546198 None:intergenic 65.0%
!! CCCCGGCACTGGTCTGGTTA+AGG + chr7_4:11546541-11546560 None:intergenic 65.0%
GTTAACCAGTGCCCCGGCAC+TGG + chr7_4:11546552-11546571 None:intergenic 65.0%
!! AGCGACCACCGATTCTCGCC+GGG + chr7_4:11545670-11545689 None:intergenic 65.0%
! TGACTAGTCCGGCCGCACTG+TGG - chr7_4:11546168-11546187 Msa1140150:CDS 65.0%
CCATGAAGCCACAGTGCGGC+CGG + chr7_4:11546179-11546198 None:intergenic 65.0%
!! CCCCGGCACTGGTCTGGTTA+AGG + chr7_4:11546541-11546560 None:intergenic 65.0%
GTTAACCAGTGCCCCGGCAC+TGG + chr7_4:11546552-11546571 None:intergenic 65.0%
CCGGCCGCACTGTGGCTTCA+TGG - chr7_4:11546176-11546195 Msa1140150:CDS 70.0%
AACTAGTGCCCCCGGGGCAC+TGG - chr7_4:11546412-11546431 Msa1140150:intron 70.0%
!! TGCCCCCGGGGCACTGGTTA+AGG - chr7_4:11546418-11546437 Msa1140150:intron 70.0%
CCGGCCGCACTGTGGCTTCA+TGG - chr7_4:11546176-11546195 Msa1140150:CDS 70.0%
AACTAGTGCCCCCGGGGCAC+TGG - chr7_4:11546412-11546431 Msa1140150:intron 70.0%
!! TGCCCCCGGGGCACTGGTTA+AGG - chr7_4:11546418-11546437 Msa1140150:intron 70.0%
!! CCAGTGCCCCGGCACTGGTC+TGG + chr7_4:11546547-11546566 None:intergenic 75.0%
! CCAGACCAGTGCCGGGGCAC+TGG - chr7_4:11546544-11546563 Msa1140150:intron 75.0%
!! CCAGTGCCCCGGCACTGGTC+TGG + chr7_4:11546547-11546566 None:intergenic 75.0%
! CCAGACCAGTGCCGGGGCAC+TGG - chr7_4:11546544-11546563 Msa1140150:intron 75.0%
Chromosome Type Strat End Strand Name
chr7_4 gene 11545567 11547523 11545567 ID=Msa1140150;Name=Msa1140150
chr7_4 mRNA 11545567 11547523 11545567 ID=Msa1140150-mRNA-1;Parent=Msa1140150;Name=Msa1140150-mRNA-1;_AED=0.00;_eAED=0.00;_QI=134|0|0.5|1|0|0.5|2|37|231
chr7_4 exon 11545567 11546299 11545567 ID=Msa1140150-mRNA-1:exon:2739;Parent=Msa1140150-mRNA-1
chr7_4 exon 11547390 11547523 11547390 ID=Msa1140150-mRNA-1:exon:2738;Parent=Msa1140150-mRNA-1
chr7_4 five_prime_UTR 11547390 11547523 11547390 ID=Msa1140150-mRNA-1:five_prime_utr;Parent=Msa1140150-mRNA-1
chr7_4 CDS 11545604 11546299 11545604 ID=Msa1140150-mRNA-1:cds;Parent=Msa1140150-mRNA-1
chr7_4 three_prime_UTR 11545567 11545603 11545567 ID=Msa1140150-mRNA-1:three_prime_utr;Parent=Msa1140150-mRNA-1
Gene Sequence

>Msa1140150

ATGAAACGTTTGCAAGAATCTGGTTCCTCTCAACAGAAACGACAAAAACTGTCGGCGCTGCCAGATGAAGAGCGTATACTCTATGACCTTATACTTAGCAAGGAAAACTCAGGGATCTCAAAAACAGACATGAGAAGAGAAACAAACCTTCCTATTGCTGCGCTTAATAAACACATGAAGGCACTTATAAACAAGAGTATGGTAAAGGAACTTCCTTCCATTAAAAACAAAGGTATAAAGATCTACATGGGAACAGAATTTGAGCCTTCCAAGGATATAACTGGTGGGCATTTTTACAGCGAAGGGAGTCTTGATACGCTTTTTATAGACGCTCTAAAGACAGTGTGCTTGCAATGCATTTCATTGGAGAGAGTTTCCACATGTGATGGATGTTTGGAGTGGGTTAAAAGGAGTGGGGTCATCACAACTCAAGTCACGGGAGAACAAATAGAAGAGATTTTGCAAACTTTGGTTTTAGATGATGAGATAATGCAGATGACAAGTACTGGATATGGGGATTTTGCATCTATTCCTGTTGGCAAAACTTGTTACATATGCAAAAGCAAGGGTGAAAAGAAATCTGCTGACTCGACTCCCTTTCCATGTTTTTCTTGTCAACGGATGAGCTTTTGTTCAACGGATGGCGTTGTCTCTCCAACAACATGTGTCTATTTTCAGAAATGGTTGGATTTCTGA
Protein sequence

>Msa1140150

MKRLQESGSSQQKRQKLSALPDEERILYDLILSKENSGISKTDMRRETNLPIAALNKHMKALINKSMVKELPSIKNKGIKIYMGTEFEPSKDITGGHFYSEGSLDTLFIDALKTVCLQCISLERVSTCDGCLEWVKRSGVITTQVTGEQIEEILQTLVLDDEIMQMTSTGYGDFASIPVGKTCYICKSKGEKKSADSTPFPCFSCQRMSFCSTDGVVSPTTCVYFQKWLDF*