AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1422230


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1422230 MtrunA17_Chr3g0094671 96.000 225 6 2 1 222 1 225 2.97e-157 434
Msa1422230 MtrunA17_Chr5g0441951 60.090 223 72 4 1 220 1 209 8.50e-87 255
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1422230 AT5G12340.1 44.385 187 94 5 33 209 22 208 1.26e-45 151
Msa1422230 AT5G12340.2 44.385 187 94 5 33 209 25 211 1.29e-45 151
Msa1422230 AT1G28190.1 27.094 203 129 6 9 203 25 216 1.36e-12 65.5

Find 32 sgRNAs with CRISPR-Local

Find 44 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCTAAGCTTGAAACTATTT+TGG 0.091162 tig0025352:+70362 Msa1422230:CDS
CGTCAGCTAAGCTTATATTT+TGG 0.226292 tig0025352:+69872 Msa1422230:CDS
AACAAGTCAACAATCAAATT+TGG 0.260285 tig0025352:+70138 Msa1422230:CDS
TTGCATGAAAAGAGATCAAT+AGG 0.299018 tig0025352:-70060 None:intergenic
TTCATCACAAGGCTTGTTAA+TGG 0.314435 tig0025352:+70225 Msa1422230:CDS
GCGTGACAAACCATCGTCTC+TGG 0.356966 tig0025352:-69955 None:intergenic
TGAAATGCTTGTGAAGTCTA+AGG 0.382530 tig0025352:+70326 Msa1422230:CDS
CTTGTTAATGGAGATAGATC+AGG 0.402456 tig0025352:+70237 Msa1422230:CDS
CAATCCTTCAAATACTCAAA+AGG 0.411864 tig0025352:-70168 None:intergenic
TCCAAAATAGTTTCAAGCTT+AGG 0.413585 tig0025352:-70363 None:intergenic
TTAGAAGACGAACTCTTTCA+AGG 0.441583 tig0025352:+70018 Msa1422230:CDS
GCTTGTGAAGTCTAAGGATC+AGG 0.441885 tig0025352:+70332 Msa1422230:CDS
TATCTAGCATACACAGTTTG+TGG 0.453920 tig0025352:-70432 None:intergenic
TCGTCTTCTAAATCCAAACT+AGG 0.463904 tig0025352:-70006 None:intergenic
TGATGGAAAAGTGTTGATAA+AGG 0.464603 tig0025352:+70191 Msa1422230:CDS
AAACAAGGTTAGACTTAGAA+AGG 0.491346 tig0025352:+70386 Msa1422230:CDS
CTAAATCCAAACTAGGGATC+GGG 0.491708 tig0025352:-69999 None:intergenic
AATCCTTCAAATACTCAAAA+GGG 0.496327 tig0025352:-70167 None:intergenic
TGAAGTCTAAGGATCAGGTT+TGG 0.505035 tig0025352:+70337 Msa1422230:CDS
TCTAAATCCAAACTAGGGAT+CGG 0.527145 tig0025352:-70000 None:intergenic
ACAAGCCTTGTGATGAACTC+TGG 0.550575 tig0025352:-70219 None:intergenic
CGTGACAAACCATCGTCTCT+GGG 0.573900 tig0025352:-69954 None:intergenic
GGTTCCACGAGATTGCTAAA+CGG 0.584296 tig0025352:+69898 Msa1422230:CDS
CGTCTTCTAAATCCAAACTA+GGG 0.587535 tig0025352:-70005 None:intergenic
TTGTTAATGGAGATAGATCA+GGG 0.611228 tig0025352:+70238 Msa1422230:CDS
TCAACTCAGGGGTACTACAA+AGG 0.612009 tig0025352:-70293 None:intergenic
GAGTATTTGAAGGATTGTGA+TGG 0.613286 tig0025352:+70174 Msa1422230:CDS
TGCATGAAAAGAGATCAATA+GGG 0.622739 tig0025352:-70059 None:intergenic
AATATAAGCTTAGCTGACGA+TGG 0.633636 tig0025352:-69868 None:intergenic
CTTACCGTTTAGCAATCTCG+TGG 0.635041 tig0025352:-69902 None:intergenic
GTTCGAGTACCCAGAGACGA+TGG 0.655665 tig0025352:+69945 Msa1422230:CDS
TTATGCCAGAGTTCATCACA+AGG 0.712497 tig0025352:+70214 Msa1422230:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GATGATAATTTTCTGTTTTG+AGG - tig0025352:69823-69842 None:intergenic 25.0%
! AACAAGTCAACAATCAAATT+TGG + tig0025352:70138-70157 Msa1422230:CDS 25.0%
! AATCCTTCAAATACTCAAAA+GGG - tig0025352:70170-70189 None:intergenic 25.0%
!!! ATAATGTTTTTTCAACTCAG+GGG - tig0025352:70307-70326 None:intergenic 25.0%
!!! CATAATGTTTTTTCAACTCA+GGG - tig0025352:70308-70327 None:intergenic 25.0%
!!! TCATAATGTTTTTTCAACTC+AGG - tig0025352:70309-70328 None:intergenic 25.0%
!!! TGAAACTATTTTGGAAAACA+AGG + tig0025352:70371-70390 Msa1422230:CDS 25.0%
TGCATGAAAAGAGATCAATA+GGG - tig0025352:70062-70081 None:intergenic 30.0%
TTGCATGAAAAGAGATCAAT+AGG - tig0025352:70063-70082 None:intergenic 30.0%
CAATCCTTCAAATACTCAAA+AGG - tig0025352:70171-70190 None:intergenic 30.0%
!! TGATGGAAAAGTGTTGATAA+AGG + tig0025352:70191-70210 Msa1422230:CDS 30.0%
TTGTTAATGGAGATAGATCA+GGG + tig0025352:70238-70257 Msa1422230:CDS 30.0%
! TCCTAAGCTTGAAACTATTT+TGG + tig0025352:70362-70381 Msa1422230:CDS 30.0%
TCCAAAATAGTTTCAAGCTT+AGG - tig0025352:70366-70385 None:intergenic 30.0%
AAACAAGGTTAGACTTAGAA+AGG + tig0025352:70386-70405 Msa1422230:CDS 30.0%
AAAGGAGAGAAAAAGAGAAA+AGG + tig0025352:70404-70423 Msa1422230:CDS 30.0%
!!! CTGTTTTGAGGATGAAAACA+TGG - tig0025352:69811-69830 None:intergenic 35.0%
AATATAAGCTTAGCTGACGA+TGG - tig0025352:69871-69890 None:intergenic 35.0%
! CGTCAGCTAAGCTTATATTT+TGG + tig0025352:69872-69891 Msa1422230:CDS 35.0%
!!! GCTAAGCTTATATTTTGGCA+AGG + tig0025352:69877-69896 Msa1422230:CDS 35.0%
TCTAAATCCAAACTAGGGAT+CGG - tig0025352:70003-70022 None:intergenic 35.0%
CGTCTTCTAAATCCAAACTA+GGG - tig0025352:70008-70027 None:intergenic 35.0%
TCGTCTTCTAAATCCAAACT+AGG - tig0025352:70009-70028 None:intergenic 35.0%
TTAGAAGACGAACTCTTTCA+AGG + tig0025352:70018-70037 Msa1422230:CDS 35.0%
GAGTATTTGAAGGATTGTGA+TGG + tig0025352:70174-70193 Msa1422230:CDS 35.0%
!! TTCATCACAAGGCTTGTTAA+TGG + tig0025352:70225-70244 Msa1422230:CDS 35.0%
CTTGTTAATGGAGATAGATC+AGG + tig0025352:70237-70256 Msa1422230:CDS 35.0%
TGAAATGCTTGTGAAGTCTA+AGG + tig0025352:70326-70345 Msa1422230:CDS 35.0%
TATCTAGCATACACAGTTTG+TGG - tig0025352:70435-70454 None:intergenic 35.0%
CTAAATCCAAACTAGGGATC+GGG - tig0025352:70002-70021 None:intergenic 40.0%
!! GTGCCCTTTTGAGTATTTGA+AGG + tig0025352:70164-70183 Msa1422230:CDS 40.0%
TTATGCCAGAGTTCATCACA+AGG + tig0025352:70214-70233 Msa1422230:CDS 40.0%
TGAAGTCTAAGGATCAGGTT+TGG + tig0025352:70337-70356 Msa1422230:CDS 40.0%
GGTTCCACGAGATTGCTAAA+CGG + tig0025352:69898-69917 Msa1422230:CDS 45.0%
CTTACCGTTTAGCAATCTCG+TGG - tig0025352:69905-69924 None:intergenic 45.0%
!!! CACGCTGACTCGTTTTTCAT+CGG + tig0025352:69973-69992 Msa1422230:CDS 45.0%
! ACAAGCCTTGTGATGAACTC+TGG - tig0025352:70222-70241 None:intergenic 45.0%
!! TCAACTCAGGGGTACTACAA+AGG - tig0025352:70296-70315 None:intergenic 45.0%
GCTTGTGAAGTCTAAGGATC+AGG + tig0025352:70332-70351 Msa1422230:CDS 45.0%
CTAAACGGTAAGCACATCGC+TGG + tig0025352:69913-69932 Msa1422230:CDS 50.0%
CGTGACAAACCATCGTCTCT+GGG - tig0025352:69957-69976 None:intergenic 50.0%
GTTCGAGTACCCAGAGACGA+TGG + tig0025352:69945-69964 Msa1422230:CDS 55.0%
GCGTGACAAACCATCGTCTC+TGG - tig0025352:69958-69977 None:intergenic 55.0%
CGGACACCCGATCCCTAGTT+TGG + tig0025352:69993-70012 Msa1422230:CDS 60.0%
Chromosome Type Strat End Strand Name
tig0025352 gene 69787 70455 69787 ID=Msa1422230;Name=Msa1422230
tig0025352 mRNA 69787 70455 69787 ID=Msa1422230-mRNA-1;Parent=Msa1422230;Name=Msa1422230-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|-1|0|1|-1|1|1|0|222
tig0025352 exon 69787 70455 69787 ID=Msa1422230-mRNA-1:exon:2452;Parent=Msa1422230-mRNA-1
tig0025352 CDS 69787 70455 69787 ID=Msa1422230-mRNA-1:cds;Parent=Msa1422230-mRNA-1
Gene Sequence

>Msa1422230

ATGGGAAATTTAGTAGTGACTCCATGTTTTCATCCTCAAAACAGAAAATTATCATCTTCATCGTCGTCGTCATCGTTGTTGCCATCGTCAGCTAAGCTTATATTTTGGCAAGGTTCCACGAGATTGCTAAACGGTAAGCACATCGCTGGCGAGATCTTGTTCGAGTACCCAGAGACGATGGTTTGTCACGCTGACTCGTTTTTCATCGGACACCCGATCCCTAGTTTGGATTTAGAAGACGAACTCTTTCAAGGAGAGACTTATTTTGTTCTCCCTATTGATCTCTTTTCATGCAAAACACTCTCAGTTTCTTCACTCTTATCATTTAGATCACATAATAATGATAGTAACAACAAGTCAACAATCAAATTTGGAGAGTGCCCTTTTGAGTATTTGAAGGATTGTGATGGAAAAGTGTTGATAAAGGTTATGCCAGAGTTCATCACAAGGCTTGTTAATGGAGATAGATCAGGGAATGATTGTTGCATTAGTAGTAAAAGTAGCTTCCTTTGTAGTACCCCTGAGTTGAAAAAACATTATGAAATGCTTGTGAAGTCTAAGGATCAGGTTTGGTCTCCTAAGCTTGAAACTATTTTGGAAAACAAGGTTAGACTTAGAAAGGAGAGAAAAAGAGAAAAGGCACAACCACAAACTGTGTATGCTAGATAG

Protein sequence

>Msa1422230

MGNLVVTPCFHPQNRKLSSSSSSSSLLPSSAKLIFWQGSTRLLNGKHIAGEILFEYPETMVCHADSFFIGHPIPSLDLEDELFQGETYFVLPIDLFSCKTLSVSSLLSFRSHNNDSNNKSTIKFGECPFEYLKDCDGKVLIKVMPEFITRLVNGDRSGNDCCISSKSSFLCSTPELKKHYEMLVKSKDQVWSPKLETILENKVRLRKERKREKAQPQTVYAR*