AlfalfaGEDB | Msa1222350

M. sativa cultivar zhongmu-4 / Msa1222350

KEGG: K09285
GO: -
PFAM: PF00847.23

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1222350	RHN64847.1	98.596	356	4	1	1	355	1	356	0.0	728

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1222350	sp\|Q94AN4\|AP2L1_ARATH	54.416	351	122	6	7	346	16	339	7.39e-106	317

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

TFs/TRs:

Gene ID	Type	Classification
Msa1222350	TF	AP2/ERF-AP2

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0005890	Msa1222350	0.815575	8.618253e-52	-8.615850e-47
Msa0028420	Msa1222350	0.813142	2.973020e-51	-8.615850e-47
Msa0081520	Msa1222350	0.824369	8.377348e-54	-8.615850e-47
Msa0614000	Msa1222350	0.819150	1.350706e-52	-8.615850e-47
Msa0626450	Msa1222350	0.837563	4.828122e-57	-8.615850e-47
Msa0626490	Msa1222350	0.837509	4.985551e-57	-8.615850e-47
Msa0638480	Msa1222350	0.830549	2.758991e-55	-8.615850e-47
Msa0644800	Msa1222350	0.805750	1.147869e-49	-8.615850e-47
Msa0663270	Msa1222350	0.806685	7.293909e-50	-8.615850e-47
Msa0664090	Msa1222350	0.843834	1.097829e-58	-8.615850e-47
Msa0664150	Msa1222350	0.820381	7.067463e-53	-8.615850e-47
Msa0664600	Msa1222350	0.835844	1.324272e-56	-8.615850e-47
Msa0683570	Msa1222350	0.824423	8.136967e-54	-8.615850e-47
Msa0795870	Msa1222350	0.801911	7.199796e-49	-8.615850e-47
Msa0833650	Msa1222350	0.801042	1.084893e-48	-8.615850e-47
Msa0835960	Msa1222350	0.800704	1.272036e-48	-8.615850e-47
Msa0173890	Msa1222350	0.813040	3.130180e-51	-8.615850e-47
Msa0225860	Msa1222350	0.806187	9.289681e-50	-8.615850e-47
Msa1005870	Msa1222350	0.813558	2.408734e-51	-8.615850e-47
Msa1044470	Msa1222350	0.827795	1.284484e-54	-8.615850e-47
Msa1092010	Msa1222350	0.812546	4.016178e-51	-8.615850e-47
Msa0267110	Msa1222350	0.802102	6.579198e-49	-8.615850e-47
Msa0300270	Msa1222350	0.823641	1.241557e-53	-8.615850e-47
Msa0300320	Msa1222350	0.814948	1.187727e-51	-8.615850e-47
Msa0337700	Msa1222350	0.814897	1.219152e-51	-8.615850e-47
Msa0339500	Msa1222350	0.810296	1.236721e-50	-8.615850e-47
Msa1132840	Msa1222350	0.806554	7.776731e-50	-8.615850e-47
Msa1157870	Msa1222350	0.811003	8.698114e-51	-8.615850e-47
Msa1217210	Msa1222350	0.842647	2.275485e-58	-8.615850e-47
Msa1222350	Msa1237740	0.819894	9.139860e-53	-8.615850e-47
Msa1222350	Msa1264710	0.896160	4.817053e-76	-8.615850e-47
Msa1222350	Msa1318170	0.817133	3.861744e-52	-8.615850e-47
Msa1222350	Msa1337900	0.908991	9.327135e-82	-8.615850e-47
Msa1222350	Msa1369150	0.809796	1.584816e-50	-8.615850e-47
Msa1222350	Msa1373100	0.802212	6.243598e-49	-8.615850e-47
Msa1222350	Msa1388600	0.825097	5.644909e-54	-8.615850e-47
Msa1222350	Msa1422950	0.801711	7.916229e-49	-8.615850e-47
Msa1222350	Msa1437940	0.804022	2.637012e-49	-8.615850e-47
Msa1222350	Msa1440960	0.823901	1.079386e-53	-8.615850e-47
Msa1222350	Msa1446810	0.810771	9.764173e-51	-8.615850e-47
Msa0527820	Msa1222350	0.832336	1.002186e-55	-8.615850e-47
Msa0580370	Msa1222350	0.807160	5.789220e-50	-8.615850e-47
Msa0582480	Msa1222350	0.849852	2.478403e-60	-8.615850e-47
Msa0586220	Msa1222350	0.849862	2.461509e-60	-8.615850e-47
Msa0597790	Msa1222350	0.831826	1.339191e-55	-8.615850e-47
Msa0861510	Msa1222350	0.806634	7.477845e-50	-8.615850e-47
Msa0861600	Msa1222350	0.804344	2.259426e-49	-8.615850e-47
Msa0881090	Msa1222350	0.976704	2.451310e-142	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1222350	MtrunA17_Chr4g0073521	98.596	356	4	1	1	355	1	356	0.0	728
Msa1222350	MtrunA17_Chr4g0002011	61.656	326	85	7	38	350	28	326	9.33e-128	369
Msa1222350	MtrunA17_Chr7g0216601	70.833	192	52	2	41	229	60	250	4.00e-98	296
Msa1222350	MtrunA17_Chr8g0355191	48.949	333	151	6	9	334	23	343	6.96e-98	296
Msa1222350	MtrunA17_Chr8g0362541	52.038	319	115	10	45	335	68	376	7.42e-94	286
Msa1222350	MtrunA17_Chr6g0452911	48.534	307	121	6	50	325	65	365	1.35e-93	284
Msa1222350	MtrunA17_Chr8g0355181	76.437	174	41	0	45	218	55	228	3.16e-92	281
Msa1222350	MtrunA17_Chr4g0072291	71.598	169	45	1	45	210	158	326	3.38e-87	272
Msa1222350	MtrunA17_Chr8g0343851	69.832	179	51	1	35	210	234	412	1.13e-85	270
Msa1222350	MtrunA17_Chr2g0328971	70.482	166	49	0	45	210	166	331	9.09e-85	266
Msa1222350	MtrunA17_Chr7g0249621	65.803	193	61	2	21	210	236	426	1.88e-84	269
Msa1222350	MtrunA17_Chr7g0249621	40.000	85	43	1	136	212	250	334	3.49e-11	64.7
Msa1222350	MtrunA17_Chr4g0032631	64.249	193	60	3	22	210	143	330	3.65e-84	265
Msa1222350	MtrunA17_Chr3g0135161	71.084	166	48	0	45	210	309	474	6.82e-83	265
Msa1222350	MtrunA17_Chr4g0054351	60.829	217	72	3	45	248	309	525	8.25e-83	264
Msa1222350	MtrunA17_Chr1g0152161	62.255	204	65	2	19	210	273	476	9.57e-83	264
Msa1222350	MtrunA17_Chr5g0400871	69.822	169	48	1	45	210	248	416	1.36e-81	258
Msa1222350	MtrunA17_Chr2g0305821	82.166	157	25	2	1	155	1	156	1.88e-75	230
Msa1222350	MtrunA17_Chr5g0412151	69.822	169	48	1	45	210	223	391	1.94e-71	231
Msa1222350	MtrunA17_Chr5g0412151	41.463	82	40	1	142	215	221	302	9.13e-11	63.2
Msa1222350	MtrunA17_Chr2g0305831	58.049	205	65	7	152	343	1	197	6.42e-66	207
Msa1222350	MtrunA17_Chr8g0367311	67.188	128	42	0	83	210	22	149	1.19e-57	187
Msa1222350	MtrunA17_Chr7g0257361	48.472	229	99	5	4	223	10	228	6.93e-53	180
Msa1222350	MtrunA17_Chr4g0053181	46.970	198	94	4	50	247	177	363	3.87e-40	148
Msa1222350	MtrunA17_Chr7g0263581	46.703	182	87	4	50	231	140	311	3.18e-39	145
Msa1222350	MtrunA17_Chr5g0401891	47.802	182	83	4	50	231	153	322	1.87e-38	143
Msa1222350	MtrunA17_Chr4g0030191	49.689	161	73	3	50	210	132	284	1.66e-37	140
Msa1222350	MtrunA17_Chr2g0325911	42.857	182	81	4	50	231	135	293	1.03e-30	121
Msa1222350	MtrunA17_Chr3g0126151	48.387	93	47	1	94	186	47	138	1.63e-22	92.0
Msa1222350	MtrunA17_Chr8g0367321	61.702	47	18	0	36	82	145	191	1.15e-14	72.0
Msa1222350	MtrunA17_Chr1g0170761	35.393	178	103	6	22	198	112	278	1.42e-14	74.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1222350	AT1G16060.1	54.416	351	122	6	7	346	16	339	7.52e-107	317
Msa1222350	AT1G79700.2	51.791	363	109	8	1	350	1	310	2.54e-99	296
Msa1222350	AT3G54320.3	74.242	198	39	3	30	215	37	234	1.56e-92	283
Msa1222350	AT3G54320.1	74.242	198	39	3	30	215	37	234	1.85e-92	283
Msa1222350	AT1G79700.1	49.036	363	109	9	1	350	1	300	9.98e-88	266
Msa1222350	AT5G57390.2	71.598	169	45	1	45	210	81	249	1.08e-86	268
Msa1222350	AT5G57390.1	71.598	169	45	1	45	210	199	367	7.37e-86	270
Msa1222350	AT1G51190.1	59.906	212	76	3	2	210	149	354	1.90e-84	266
Msa1222350	AT5G17430.2	66.667	189	55	2	25	210	129	312	1.62e-83	263
Msa1222350	AT5G17430.1	66.667	189	55	2	25	210	191	374	2.90e-83	264
Msa1222350	AT1G72570.1	66.304	184	56	2	33	210	204	387	3.33e-83	259
Msa1222350	AT3G20840.1	67.456	169	52	1	45	210	177	345	3.06e-81	258
Msa1222350	AT4G37750.1	68.047	169	51	1	45	210	279	447	2.35e-77	248
Msa1222350	AT5G65510.4	69.231	169	49	1	45	210	169	337	1.75e-72	232
Msa1222350	AT5G10510.2	69.277	166	51	0	45	210	226	391	2.26e-72	234
Msa1222350	AT5G65510.2	69.231	169	49	1	45	210	227	395	3.12e-72	233
Msa1222350	AT5G65510.1	69.231	169	49	1	45	210	227	395	3.12e-72	233
Msa1222350	AT5G10510.7	68.047	169	51	1	45	210	226	394	3.47e-70	229
Msa1222350	AT5G10510.5	68.047	169	51	1	45	210	226	394	3.47e-70	229
Msa1222350	AT5G10510.1	68.047	169	51	1	45	210	249	417	6.58e-70	229
Msa1222350	AT5G10510.6	68.047	169	51	1	45	210	264	432	7.01e-70	229
Msa1222350	AT5G10510.4	68.047	169	51	1	45	210	264	432	7.01e-70	229
Msa1222350	AT1G16060.3	52.941	272	93	5	83	346	25	269	2.17e-67	214
Msa1222350	AT1G79700.4	50.545	275	76	6	83	350	7	228	9.03e-66	208
Msa1222350	AT1G79700.3	50.545	275	76	6	83	350	13	234	1.18e-65	208
Msa1222350	AT3G54320.4	80.451	133	26	0	83	215	36	168	1.79e-65	211
Msa1222350	AT3G54320.5	80.451	133	26	0	83	215	36	168	1.79e-65	211
Msa1222350	AT5G10510.3	60.317	189	52	1	45	210	264	452	1.86e-65	218
Msa1222350	AT5G65510.3	68.345	139	41	1	45	180	169	307	4.57e-65	209
Msa1222350	AT1G16060.2	50.000	232	81	5	123	346	1	205	7.20e-53	174
Msa1222350	AT1G16060.4	50.000	232	81	5	123	346	1	205	7.20e-53	174
Msa1222350	AT1G16060.6	50.000	232	81	5	123	346	1	205	7.20e-53	174
Msa1222350	AT1G16060.5	50.000	232	81	5	123	346	1	205	7.20e-53	174
Msa1222350	AT2G41710.1	52.083	192	80	3	31	219	52	234	1.91e-52	179
Msa1222350	AT2G41710.4	52.083	192	80	3	31	219	52	234	2.53e-52	180
Msa1222350	AT2G41710.2	50.761	197	80	4	31	219	52	239	6.88e-50	172
Msa1222350	AT2G41710.3	44.053	227	80	4	31	219	52	269	1.78e-45	162
Msa1222350	AT3G54320.2	82.796	93	16	0	123	215	1	93	1.69e-42	149
Msa1222350	AT2G28550.3	46.000	200	94	4	50	249	154	339	3.31e-40	147
Msa1222350	AT4G36920.1	43.721	215	107	5	50	258	132	338	2.21e-39	145
Msa1222350	AT4G36920.2	43.721	215	107	5	50	258	132	338	2.21e-39	145
Msa1222350	AT5G67180.2	45.946	185	83	4	35	210	73	249	4.19e-38	138
Msa1222350	AT5G67180.3	45.946	185	83	4	35	210	73	249	7.62e-38	137
Msa1222350	AT5G67180.1	45.946	185	83	4	35	210	73	249	2.26e-37	137
Msa1222350	AT2G28550.4	48.905	137	62	3	50	186	154	282	1.33e-31	122
Msa1222350	AT2G28550.1	42.500	200	86	5	50	249	154	324	5.34e-31	122
Msa1222350	AT1G16060.7	43.069	202	80	5	153	346	9	183	5.25e-30	114
Msa1222350	AT5G67180.4	43.293	164	76	4	35	189	73	228	1.35e-28	112
Msa1222350	AT2G28550.2	48.780	123	55	3	50	172	154	268	1.10e-25	104
Msa1222350	AT2G28550.6	48.780	123	55	3	50	172	154	268	1.10e-25	104
Msa1222350	AT2G28550.5	41.916	167	75	3	83	249	14	158	1.26e-23	99.4
Msa1222350	AT5G60120.4	37.017	181	89	5	23	203	136	291	2.30e-21	94.7
Msa1222350	AT5G60120.3	37.017	181	89	5	23	203	136	291	2.30e-21	94.7
Msa1222350	AT5G60120.5	37.017	181	89	5	23	203	136	291	2.30e-21	94.7
Msa1222350	AT5G60120.6	37.017	181	89	5	23	203	136	291	4.59e-21	94.4
Msa1222350	AT5G60120.1	37.017	181	89	5	23	203	136	291	4.60e-21	94.4
Msa1222350	AT5G60120.2	37.017	181	89	5	23	203	136	291	4.92e-21	94.7
Msa1222350	AT2G39250.2	43.564	101	50	2	50	150	109	202	5.30e-20	88.2
Msa1222350	AT2G39250.1	43.564	101	50	2	50	150	109	202	1.41e-19	88.6
Msa1222350	AT3G54990.2	43.269	104	52	2	50	153	123	219	1.07e-15	76.3
Msa1222350	AT3G54990.1	43.269	104	52	2	50	153	123	219	3.59e-15	76.3

Find 99 sgRNAs with CRISPR-Local

Find 302 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GCTTCAGTCATCTAAGTTTA+AGG	0.098001	8_1:-86624977	Msa1222350:CDS
TCTTTATAAATTAGCTAATT+AGG	0.158119	8_1:-86624673	Msa1222350:three_prime_UTR
GCTCAATTCAAAAGAGAATT+TGG	0.171485	8_1:-86624761	Msa1222350:three_prime_UTR
ATATTTATATATAGTATTAA+TGG	0.232581	8_1:+86629945	None:intergenic
ATCTAGCAGCATTGAAATAT+TGG	0.269149	8_1:-86628940	Msa1222350:CDS
AAGCTGTAGCTGCTTCTTCT+TGG	0.308304	8_1:+86625286	None:intergenic
ATAACGGCTGAGATCGAAAT+TGG	0.311170	8_1:+86625221	None:intergenic
CGTTGAGGTGGAGAAGTTCT+AGG	0.318274	8_1:+86629761	None:intergenic
TTGGGTGAAGAAGAATTTAA+AGG	0.321665	8_1:+86630024	None:intergenic
CATTAAGCTTACAAATAAAA+AGG	0.328490	8_1:-86630225	Msa1222350:five_prime_UTR
GTCATCGTTGCTATTATTGT+TGG	0.330644	8_1:+86625182	None:intergenic
GATTAATACTCACCTTCTTA+AGG	0.332089	8_1:+86628738	None:intergenic
ATGAATTATAGTATAGTGTT+TGG	0.332488	8_1:+86630005	None:intergenic
AAAGGGATGGGCTAAGGAAT+GGG	0.338837	8_1:+86630042	None:intergenic
GTCATCTAAGTTTAAGGAAA+TGG	0.343211	8_1:-86624971	Msa1222350:CDS
TCTAGCAGCATTGAAATATT+GGG	0.363825	8_1:-86628939	Msa1222350:CDS
AGCTGTAGCTGCTTCTTCTT+GGG	0.378837	8_1:+86625287	None:intergenic
AATGTCACAAAGATTAATAT+TGG	0.380390	8_1:+86625149	None:intergenic
CAATCAAGGGAGGAGTATAT+TGG	0.384836	8_1:-86628763	Msa1222350:CDS
ATCTCAGCCGTTATATTAAG+TGG	0.390059	8_1:-86625212	Msa1222350:CDS
ACAGTCAAATTCAATACAAT+TGG	0.396468	8_1:+86624597	None:intergenic
CTCAAACTCTAATGAAGAAC+TGG	0.396693	8_1:-86625103	Msa1222350:CDS
GTTTAAGGAAATGGTGGAAA+TGG	0.403137	8_1:-86624962	Msa1222350:CDS
TGAATTATAGTATAGTGTTT+GGG	0.403305	8_1:+86630006	None:intergenic
ACGTTCCATTATGACTTTGA+AGG	0.409164	8_1:-86624799	Msa1222350:CDS
AATTACCGGAAAATTAATAA+TGG	0.409895	8_1:+86628907	None:intergenic
GGCAATAAGTATCTTTATCT+CGG	0.416691	8_1:-86627714	Msa1222350:CDS
TATCTTTATCTCGGAACATA+TGG	0.418592	8_1:-86627705	Msa1222350:intron
TTTGTGGCCGTCTGTAAAAT+CGG	0.425770	8_1:+86630117	None:intergenic
TCATCGTTGCTATTATTGTT+GGG	0.426434	8_1:+86625183	None:intergenic
TGGGTGAAGAAGAATTTAAA+GGG	0.432032	8_1:+86630025	None:intergenic
TTCATTAGAGTTTGAGTCGT+TGG	0.432547	8_1:+86625110	None:intergenic
ATTTGTGGGACAAGAATTGT+TGG	0.438001	8_1:-86629547	Msa1222350:CDS
CAGCACCTTCAAAGTCATAA+TGG	0.453462	8_1:+86624794	None:intergenic
GTTTAAGCCACTTAATATAA+CGG	0.455696	8_1:+86625205	None:intergenic
TAAAGGGATGGGCTAAGGAA+TGG	0.457937	8_1:+86630041	None:intergenic
GTCGATATGAAGCTCATTTG+TGG	0.463814	8_1:-86629562	Msa1222350:CDS
ATAATGGAACGTAGGCATAA+TGG	0.466327	8_1:+86624810	None:intergenic
AGGGCTGATGTGGCACTAGT+TGG	0.472897	8_1:+86625009	None:intergenic
TAATGGTGCATTAATTTGAA+AGG	0.481532	8_1:+86629962	None:intergenic
TCGATATGAAGCTCATTTGT+GGG	0.483951	8_1:-86629561	Msa1222350:CDS
AAGTACGTTTGAATGTCATC+AGG	0.487256	8_1:+86624877	None:intergenic
ATGACTGAAGCAAAAGCTCT+AGG	0.492154	8_1:+86624989	None:intergenic
AATGAGTCTCAGAACAAGAA+AGG	0.503226	8_1:-86629524	Msa1222350:CDS
GGAAGGTGGGAGGCACGGAT+TGG	0.503282	8_1:-86627747	Msa1222350:CDS
TTCAGGTGCTTATGATAATG+AGG	0.506979	8_1:-86628984	Msa1222350:intron
TTCTATTGACGAAGCTACAT+TGG	0.508550	8_1:-86625043	Msa1222350:CDS
TCGATCAGGCACCGTTGGAC+AGG	0.512931	8_1:-86629584	Msa1222350:intron
GAATTTAAAGGGATGGGCTA+AGG	0.517366	8_1:+86630036	None:intergenic
ATGAGTCTCAGAACAAGAAA+GGG	0.517662	8_1:-86629523	Msa1222350:CDS
TGACTGAAGCAAAAGCTCTA+GGG	0.520622	8_1:+86624990	None:intergenic
TGAAGAAGAATTTAAAGGGA+TGG	0.528945	8_1:+86630029	None:intergenic
CAAAAGCTCTAGGGCTGATG+TGG	0.531124	8_1:+86624999	None:intergenic
TTGAAATGGAGAGTCAATCA+AGG	0.532530	8_1:-86628777	Msa1222350:CDS
AGCTAATTTATAAAGAGTAT+CGG	0.535039	8_1:+86624680	None:intergenic
GGCTGAAAGATGGTTGTTTG+TGG	0.538989	8_1:+86630101	None:intergenic
AAGGGATGGGCTAAGGAATG+GGG	0.543605	8_1:+86630043	None:intergenic
GACATCATCATAACGGAAGG+TGG	0.545087	8_1:-86627761	Msa1222350:CDS
CATATATATAGCTATTGTAA+AGG	0.551463	8_1:+86624560	None:intergenic
TACCATCGATCAGGCACCGT+TGG	0.551468	8_1:-86629589	Msa1222350:intron
GAAGAAGAATTTAAAGGGAT+GGG	0.551603	8_1:+86630030	None:intergenic
AGTATATTGGATCCTTAAGA+AGG	0.558908	8_1:-86628750	Msa1222350:intron
CTGTCACCACCAAAGCAAAA+CGG	0.561267	8_1:-86629799	Msa1222350:CDS
ATCTAAGTTTAAGGAAATGG+TGG	0.564662	8_1:-86624968	Msa1222350:CDS
CCACCAAAGCAAAACGGACG+AGG	0.564803	8_1:-86629793	Msa1222350:CDS
TGCTTATGATAATGAGGAGA+CGG	0.567000	8_1:-86628978	Msa1222350:CDS
TGTGATAGCCGGCTGAAAGA+TGG	0.577707	8_1:+86630091	None:intergenic
TTATGACTTTGAAGGTGCTG+AGG	0.583578	8_1:-86624791	Msa1222350:CDS
CAATATACAGAGGAGTCACT+AGG	0.587159	8_1:-86629733	Msa1222350:intron
AAATTTCAATGATACCATGT+TGG	0.588011	8_1:-86624845	Msa1222350:CDS
ACAAACAACCATCTTTCAGC+CGG	0.591544	8_1:-86630099	Msa1222350:five_prime_UTR
GTTGAGGTGGAGAAGTTCTA+GGG	0.592000	8_1:+86629762	None:intergenic
TGAAATGGAGAGTCAATCAA+GGG	0.593771	8_1:-86628776	Msa1222350:CDS
CTGTATATTGAGCTGCGTTG+AGG	0.594111	8_1:+86629746	None:intergenic
CTCCAACGTTGTTGACACAT+TGG	0.595590	8_1:+86624927	None:intergenic
TGTTCTAGACATCATCATAA+CGG	0.603537	8_1:-86627768	Msa1222350:intron
ATAACGGAAGGTGGGAGGCA+CGG	0.604592	8_1:-86627752	Msa1222350:CDS
TATATTGAGCTGCGTTGAGG+TGG	0.606937	8_1:+86629749	None:intergenic
CAGAACAAGAAAGGGAGACA+AGG	0.613931	8_1:-86629515	Msa1222350:intron
ACATCATCATAACGGAAGGT+GGG	0.615401	8_1:-86627760	Msa1222350:CDS
ACGTAGGCATAATGGAGTTG+AGG	0.618652	8_1:+86624818	None:intergenic
TGGTAACAGCATTAAGTCCA+CGG	0.621825	8_1:+86625241	None:intergenic
CTAGACATCATCATAACGGA+AGG	0.629308	8_1:-86627764	Msa1222350:intron
TTCCAATGTGTCAACAACGT+TGG	0.630167	8_1:-86624929	Msa1222350:CDS
CTAAATACAGAGGTGTAGCA+AGG	0.631468	8_1:-86628603	Msa1222350:intron
CTAGCAGCATTGAAATATTG+GGG	0.632471	8_1:-86628938	Msa1222350:CDS
GCTGGCAGAATTGTGATAGC+CGG	0.634528	8_1:+86630080	None:intergenic
CTTCATATCGACCTGTCCAA+CGG	0.639587	8_1:+86629573	None:intergenic
GATGTGGCACTAGTTGGTCG+AGG	0.640586	8_1:+86625015	None:intergenic
GGAGTTGAGGTCTTCCAACA+TGG	0.661453	8_1:+86624831	None:intergenic
AGCAGCTACAGCTTATGACA+TGG	0.665369	8_1:-86625277	Msa1222350:CDS
TCAAAGTCATAATGGAACGT+AGG	0.669979	8_1:+86624802	None:intergenic
TCATCATAACGGAAGGTGGG+AGG	0.671171	8_1:-86627757	Msa1222350:CDS
GTGATATTTCATTGTCCACG+AGG	0.678307	8_1:+86625073	None:intergenic
CGGGGAGTATCTAAATACAG+AGG	0.707462	8_1:-86628613	Msa1222350:CDS
ATGGCAGCTATTGAATACCG+TGG	0.709584	8_1:-86625258	Msa1222350:CDS
AATGGAGAGTCAATCAAGGG+AGG	0.710747	8_1:-86628773	Msa1222350:CDS
AGAACTGGAATTTAGCCTCG+TGG	0.711966	8_1:-86625088	Msa1222350:CDS
CAACGCAGCTCAATATACAG+AGG	0.725894	8_1:-86629743	Msa1222350:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTATTAATTTATGATTTTCT+TGG	-	chr8_1:86626557-86626576	Msa1222350:intron	10.0%
!!!	TATTAATTTATGATTTTCTT+GGG	-	chr8_1:86626558-86626577	Msa1222350:intron	10.0%
!!!	TAATAAGATAATAATTTTTC+GGG	+	chr8_1:86626606-86626625	None:intergenic	10.0%
!!	TAAGAAAAATGTATAAATAA+AGG	-	chr8_1:86627607-86627626	Msa1222350:intron	10.0%
!!	AAGAAAAATGTATAAATAAA+GGG	-	chr8_1:86627608-86627627	Msa1222350:intron	10.0%
!!!	ATTCTTAAAATAAGTTTTAT+AGG	+	chr8_1:86628046-86628065	None:intergenic	10.0%
!!	TTAGCTATATATATTAATAT+TGG	+	chr8_1:86629322-86629341	None:intergenic	10.0%
!!!	CATTTTGAAACAATTTTTTT+TGG	+	chr8_1:86625483-86625502	None:intergenic	15.0%
!!!	TAGTTTTAAAAGTAATACTT+TGG	+	chr8_1:86625586-86625605	None:intergenic	15.0%
!!	AAATTATCCTTATTAACTAT+TGG	+	chr8_1:86625610-86625629	None:intergenic	15.0%
!!	AATAGTTAATAAGGATAATT+TGG	-	chr8_1:86625609-86625628	Msa1222350:intron	15.0%
!!	TCTAAATTAATAAATTTCTC+CGG	+	chr8_1:86626272-86626291	None:intergenic	15.0%
!!!	AGTTTTTCAATTTTTTACAT+TGG	-	chr8_1:86626634-86626653	Msa1222350:intron	15.0%
!!!	TCTAAAATAATAAATTTGTC+CGG	+	chr8_1:86626672-86626691	None:intergenic	15.0%
!!	AGAAAAATGTATAAATAAAG+GGG	-	chr8_1:86627609-86627628	Msa1222350:intron	15.0%
!!	TAATTAAATACGATAAAAGT+TGG	+	chr8_1:86627916-86627935	None:intergenic	15.0%
!!!	TAAAAATTTATCTTTTCCAA+AGG	-	chr8_1:86627944-86627963	Msa1222350:intron	15.0%
!!	AACCCATATATATATATATA+AGG	-	chr8_1:86628467-86628486	Msa1222350:intron	15.0%
!!	ACCCATATATATATATATAA+GGG	-	chr8_1:86628468-86628487	Msa1222350:intron	15.0%
!!	ACCCTTATATATATATATAT+GGG	+	chr8_1:86628472-86628491	None:intergenic	15.0%
!!	AACCCTTATATATATATATA+TGG	+	chr8_1:86628473-86628492	None:intergenic	15.0%
!!	AATGCTATTAAAATTAAGTA+GGG	+	chr8_1:86628955-86628974	None:intergenic	15.0%
!!	AAATGCTATTAAAATTAAGT+AGG	+	chr8_1:86628954-86628973	None:intergenic	15.0%
!!	AGATAAAAAGTAATAAAATC+TGG	+	chr8_1:86628734-86628753	None:intergenic	15.0%
!!	TTCAAAAATTTAAGAGTTTA+AGG	-	chr8_1:86628865-86628884	Msa1222350:intron	15.0%
!!	AAATGCTATTAAAATTAAGT+AGG	-	chr8_1:86628954-86628973	Msa1222350:CDS	15.0%
!!	AATGCTATTAAAATTAAGTA+GGG	-	chr8_1:86628955-86628974	Msa1222350:CDS	15.0%
!!	ATAATTAACCCTATATATAA+AGG	-	chr8_1:86629018-86629037	Msa1222350:intron	15.0%
!!!	TAAAAATTTGATAATATGCA+TGG	-	chr8_1:86629187-86629206	Msa1222350:intron	15.0%
!!!	AAAAATTTGATAATATGCAT+GGG	-	chr8_1:86629188-86629207	Msa1222350:intron	15.0%
!!	TCTTTATAAATTAGCTAATT+AGG	-	chr8_1:86630109-86630128	Msa1222350:five_prime_UTR	15.0%
!!	CATTAAGCTTACAAATAAAA+AGG	-	chr8_1:86624557-86624576	Msa1222350:three_prime_UTR	20.0%
!!	TGAATTATAGTATAGTGTTT+GGG	+	chr8_1:86624779-86624798	None:intergenic	20.0%
!!	ATGAATTATAGTATAGTGTT+TGG	+	chr8_1:86624780-86624799	None:intergenic	20.0%
!!	AGAAAAAGTTATTATTGCAT+TGG	+	chr8_1:86625523-86625542	None:intergenic	20.0%
!!	AAACTAACCAATAGTTAATA+AGG	-	chr8_1:86625600-86625619	Msa1222350:intron	20.0%
!!!	AAGTACTTAAATGATTCTTT+TGG	+	chr8_1:86625755-86625774	None:intergenic	20.0%
!!!	AGATACCATTATTAATTTTC+CGG	-	chr8_1:86625870-86625889	Msa1222350:intron	20.0%
!!	AATTACCGGAAAATTAATAA+TGG	+	chr8_1:86625878-86625897	None:intergenic	20.0%
!!	TCAAAAAGAACTAATTGAAA+TGG	-	chr8_1:86625991-86626010	Msa1222350:intron	20.0%
!!!	TATAGAGTTTTAACTGTATA+AGG	+	chr8_1:86626232-86626251	None:intergenic	20.0%
!!	CTCTATAAATTAATAATGTC+AGG	-	chr8_1:86626245-86626264	Msa1222350:intron	20.0%
!!!	ATGTATCTAGTCTTTATTAT+AGG	-	chr8_1:86626882-86626901	Msa1222350:intron	20.0%
!!!	TCATTTTGTAGTAAAAGAAA+AGG	+	chr8_1:86627154-86627173	None:intergenic	20.0%
!!!	GTACTAATAATTTTTTTGAG+AGG	+	chr8_1:86627268-86627287	None:intergenic	20.0%
!!	AAAGTGGAATATATTGTATT+GGG	-	chr8_1:86627632-86627651	Msa1222350:intron	20.0%
!!!	GACACTTTTTTTTAGAAAAT+GGG	-	chr8_1:86627742-86627761	Msa1222350:CDS	20.0%
!!!	TTTTTCCTTATAATTTGAGA+CGG	+	chr8_1:86627854-86627873	None:intergenic	20.0%
!!!	GGTCTTTTTGTTTATAATTT+GGG	+	chr8_1:86627895-86627914	None:intergenic	20.0%
!!!	TGGTCTTTTTGTTTATAATT+TGG	+	chr8_1:86627896-86627915	None:intergenic	20.0%
!!!	AAACAATTTTCATTGGTTAT+GGG	+	chr8_1:86628014-86628033	None:intergenic	20.0%
!!!	AAAACAATTTTCATTGGTTA+TGG	+	chr8_1:86628015-86628034	None:intergenic	20.0%
!!!	GATGTAAAAACAATTTTCAT+TGG	+	chr8_1:86628021-86628040	None:intergenic	20.0%
!!	AAAATCACACTTATTAAGAA+AGG	+	chr8_1:86628108-86628127	None:intergenic	20.0%
!!!	TTTTTCCCTTTCTTATAATA+TGG	-	chr8_1:86628126-86628145	Msa1222350:intron	20.0%
!!!	TTTTCCCTTTCTTATAATAT+GGG	-	chr8_1:86628127-86628146	Msa1222350:intron	20.0%
!!!	ACTCATTCTAAAAAATAAGT+GGG	+	chr8_1:86628350-86628369	None:intergenic	20.0%
!!	AAGTTGTTAAATACATCTTA+AGG	+	chr8_1:86628389-86628408	None:intergenic	20.0%
!!	AATGCAGAAAAACAAAATTA+GGG	+	chr8_1:86628571-86628590	None:intergenic	20.0%
!!	AAACAAATTTGAATTGACTA+GGG	+	chr8_1:86628604-86628623	None:intergenic	20.0%
!!	TAAACAAATTTGAATTGACT+AGG	+	chr8_1:86628605-86628624	None:intergenic	20.0%
!!!	AATTTTGCAATAACTTTTTG+CGG	+	chr8_1:86628699-86628718	None:intergenic	20.0%
!!	ATCTTATCTGACATTAAAAT+TGG	-	chr8_1:86629429-86629448	Msa1222350:intron	20.0%
!!	AATGTCACAAAGATTAATAT+TGG	+	chr8_1:86629636-86629655	None:intergenic	20.0%
!!!	AGCTAATTTATAAAGAGTAT+CGG	+	chr8_1:86630105-86630124	None:intergenic	20.0%
!!	CATATATATAGCTATTGTAA+AGG	+	chr8_1:86630225-86630244	None:intergenic	20.0%
!!	TAATGGTGCATTAATTTGAA+AGG	+	chr8_1:86624823-86624842	None:intergenic	25.0%
!!	TACACTTCTTTTTATTCCAA+TGG	-	chr8_1:86624888-86624907	Msa1222350:CDS	25.0%
!	TAGATAAACATATACAAGAG+AGG	+	chr8_1:86625111-86625130	None:intergenic	25.0%
!!!	TGTTTTAACTTCTTTTCATG+TGG	-	chr8_1:86625354-86625373	Msa1222350:intron	25.0%
!	TATATATAAACTACGTACCA+AGG	+	chr8_1:86625428-86625447	None:intergenic	25.0%
!	AATGATTAAGAGAAAGAGAA+TGG	+	chr8_1:86625643-86625662	None:intergenic	25.0%
!	AAAAAGTGGTTAGAAATTAC+CGG	+	chr8_1:86625892-86625911	None:intergenic	25.0%
!	TGGATGCATATTAAAAAAAG+TGG	+	chr8_1:86625906-86625925	None:intergenic	25.0%
!!!	ACTTTTTTTAATATGCATCC+AGG	-	chr8_1:86625905-86625924	Msa1222350:intron	25.0%
!	CTGAACTAATTAATAAATCC+TGG	+	chr8_1:86625926-86625945	None:intergenic	25.0%
!	TAAATTAATAATGTCAGGAC+CGG	-	chr8_1:86626250-86626269	Msa1222350:intron	25.0%
!	GTACAGTAAATGTAAATAAG+AGG	-	chr8_1:86626483-86626502	Msa1222350:intron	25.0%
!	TTCTTAATTACACTCTTGTT+TGG	+	chr8_1:86626783-86626802	None:intergenic	25.0%
!!	TAAGCAAAACTTCATTTTCT+AGG	-	chr8_1:86626844-86626863	Msa1222350:intron	25.0%
!	AGGGAGTAAAAATCAATTTA+AGG	-	chr8_1:86626966-86626985	Msa1222350:intron	25.0%
!	TAAATTTAGATTGTTGTACC+TGG	+	chr8_1:86627309-86627328	None:intergenic	25.0%
!	AGGTACAACAATCTAAATTT+AGG	-	chr8_1:86627308-86627327	Msa1222350:intron	25.0%
!	ACAACAATCTAAATTTAGGT+AGG	-	chr8_1:86627312-86627331	Msa1222350:intron	25.0%
!	AATAGAAGCAATGAATTGAA+TGG	+	chr8_1:86627413-86627432	None:intergenic	25.0%
!	GAAAGTGGAATATATTGTAT+TGG	-	chr8_1:86627631-86627650	Msa1222350:intron	25.0%
!!	TTGAAATGACTCAGTTATTT+TGG	-	chr8_1:86627719-86627738	Msa1222350:CDS	25.0%
!!!	TGAAATGACTCAGTTATTTT+GGG	-	chr8_1:86627720-86627739	Msa1222350:CDS	25.0%
!!!	GGACACTTTTTTTTAGAAAA+TGG	-	chr8_1:86627741-86627760	Msa1222350:CDS	25.0%
!!!	GAATGCATTTAATTTTCCTT+TGG	+	chr8_1:86627963-86627982	None:intergenic	25.0%
!!	ACACCTTCTAAAAAATAAGT+GGG	-	chr8_1:86628285-86628304	Msa1222350:intron	25.0%
!!	CACTCATTCTAAAAAATAAG+TGG	+	chr8_1:86628351-86628370	None:intergenic	25.0%
!	ACACCTTAATAAAAACTAGT+AGG	-	chr8_1:86628432-86628451	Msa1222350:intron	25.0%
!!!	ACACCTACTAGTTTTTATTA+AGG	+	chr8_1:86628438-86628457	None:intergenic	25.0%
!!	TATATATATATGGGTTTGCT+AGG	+	chr8_1:86628463-86628482	None:intergenic	25.0%
!	CAATGCAGAAAAACAAAATT+AGG	+	chr8_1:86628572-86628591	None:intergenic	25.0%
!!!	TAATCAAACTTTGTTTTAGC+AGG	-	chr8_1:86628893-86628912	Msa1222350:intron	25.0%
!!!	AATCAAACTTTGTTTTAGCA+GGG	-	chr8_1:86628894-86628913	Msa1222350:intron	25.0%
!	AATCCTCTCCTTTATATATA+GGG	+	chr8_1:86629029-86629048	None:intergenic	25.0%
!	TTCTTTGGTATCTATGAAAT+CGG	-	chr8_1:86629047-86629066	Msa1222350:intron	25.0%
!	ATTTAAACACAACTCACTTA+AGG	+	chr8_1:86629226-86629245	None:intergenic	25.0%
!!!	TGTTTGTTGTTGAGAAAAAT+TGG	+	chr8_1:86629381-86629400	None:intergenic	25.0%
!!	CAGATAAGATATTGATTAGT+CGG	+	chr8_1:86629419-86629438	None:intergenic	25.0%
!	GTTTAAGCCACTTAATATAA+CGG	+	chr8_1:86629580-86629599	None:intergenic	25.0%
!	AAATTTCAATGATACCATGT+TGG	-	chr8_1:86629937-86629956	Msa1222350:five_prime_UTR	25.0%
!!	ATTAGGATTTAAGCTTTTAG+AGG	-	chr8_1:86630126-86630145	Msa1222350:five_prime_UTR	25.0%
!	ACAGTCAAATTCAATACAAT+TGG	+	chr8_1:86630188-86630207	None:intergenic	25.0%
	GAAGAAGAATTTAAAGGGAT+GGG	+	chr8_1:86624755-86624774	None:intergenic	30.0%
	TGAAGAAGAATTTAAAGGGA+TGG	+	chr8_1:86624756-86624775	None:intergenic	30.0%
	TGGGTGAAGAAGAATTTAAA+GGG	+	chr8_1:86624760-86624779	None:intergenic	30.0%
	TTGGGTGAAGAAGAATTTAA+AGG	+	chr8_1:86624761-86624780	None:intergenic	30.0%
	AGAGAGGAAATAAACTATCA+AGG	+	chr8_1:86625095-86625114	None:intergenic	30.0%
	AAAAAATGTTACCATCGATC+AGG	-	chr8_1:86625184-86625203	Msa1222350:CDS	30.0%
!!!	TTCAAAATGAGTGTCGTTTT+AGG	+	chr8_1:86625691-86625710	None:intergenic	30.0%
!!	AAACGACACTCATTTTGAAA+CGG	-	chr8_1:86625692-86625711	Msa1222350:intron	30.0%
	GAGCTTGATGTTAAAATTTC+AGG	-	chr8_1:86625781-86625800	Msa1222350:intron	30.0%
!	ATCTAGCAGCATTGAAATAT+TGG	-	chr8_1:86625842-86625861	Msa1222350:intron	30.0%
!	TCTAGCAGCATTGAAATATT+GGG	-	chr8_1:86625843-86625862	Msa1222350:intron	30.0%
	AGTATATTGGATCCTTAAGA+AGG	-	chr8_1:86626032-86626051	Msa1222350:intron	30.0%
	GATTAATACTCACCTTCTTA+AGG	+	chr8_1:86626047-86626066	None:intergenic	30.0%
	AAAAATTGCAGAAAAAGCAG+TGG	-	chr8_1:86626139-86626158	Msa1222350:intron	30.0%
	ACTGTACATACCAAACAAAA+AGG	-	chr8_1:86626352-86626371	Msa1222350:intron	30.0%
!!	GACTAATTTGCCTTTTTGTT+TGG	+	chr8_1:86626365-86626384	None:intergenic	30.0%
	TTGGGACCGAAAATTATAAA+GGG	-	chr8_1:86626576-86626595	Msa1222350:intron	30.0%
!	GGAGATCCCTTTATAATTTT+CGG	+	chr8_1:86626585-86626604	None:intergenic	30.0%
!	TTTTACTCAATTACTCTCTC+CGG	-	chr8_1:86626812-86626831	Msa1222350:intron	30.0%
!	GTTTTGCTTATAATAGTGAC+CGG	+	chr8_1:86626834-86626853	None:intergenic	30.0%
!!	AAATTGATTTTTACTCCCTC+TGG	+	chr8_1:86626965-86626984	None:intergenic	30.0%
!	GATGATGTCTAGAACATTAA+TGG	+	chr8_1:86627010-86627029	None:intergenic	30.0%
!	TGTTCTAGACATCATCATAA+CGG	-	chr8_1:86627014-86627033	Msa1222350:intron	30.0%
	GGCAATAAGTATCTTTATCT+CGG	-	chr8_1:86627068-86627087	Msa1222350:intron	30.0%
	TATCTTTATCTCGGAACATA+TGG	-	chr8_1:86627077-86627096	Msa1222350:intron	30.0%
	TGTACGTATAAATGATCGTA+TGG	-	chr8_1:86627203-86627222	Msa1222350:intron	30.0%
!	AATTGAATGGCACTTTTCTA+AGG	+	chr8_1:86627400-86627419	None:intergenic	30.0%
	CTATGGAGTAATAACTGAAT+TGG	+	chr8_1:86627442-86627461	None:intergenic	30.0%
	TGTATAAATAAAGGGGAAAG+TGG	-	chr8_1:86627616-86627635	Msa1222350:intron	30.0%
!!!	TTCTCTTGCTATTTTTTTCG+TGG	+	chr8_1:86627809-86627828	None:intergenic	30.0%
!	ACACCCACTAATTTTTATGT+GGG	-	chr8_1:86628198-86628217	Msa1222350:intron	30.0%
	ACTCCCACATAAAAATTAGT+GGG	+	chr8_1:86628204-86628223	None:intergenic	30.0%
	AAGTAGTTAAATACACCCTA+AGG	+	chr8_1:86628243-86628262	None:intergenic	30.0%
!	CACACCTTCTAAAAAATAAG+TGG	-	chr8_1:86628284-86628303	Msa1222350:intron	30.0%
	GTATTATAGCAAATGCCTAT+AGG	-	chr8_1:86628309-86628328	Msa1222350:intron	30.0%
!!	TTCAAGGTCGCTATTTTATA+GGG	+	chr8_1:86628511-86628530	None:intergenic	30.0%
!!	TTTCAAGGTCGCTATTTTAT+AGG	+	chr8_1:86628512-86628531	None:intergenic	30.0%
	CAAGATTAGAACACTATGAA+AGG	+	chr8_1:86628831-86628850	None:intergenic	30.0%
!	CACAAGGTCTCTATTTTATA+AGG	-	chr8_1:86628986-86629005	Msa1222350:intron	30.0%
	TAACCCTATATATAAAGGAG+AGG	-	chr8_1:86629023-86629042	Msa1222350:intron	30.0%
	GAATCCTCTCCTTTATATAT+AGG	+	chr8_1:86629030-86629049	None:intergenic	30.0%
	ATATAAAGGAGAGGATTCTT+TGG	-	chr8_1:86629032-86629051	Msa1222350:intron	30.0%
	TAATATGCATGGGTGTATAT+TGG	-	chr8_1:86629198-86629217	Msa1222350:intron	30.0%
!	TCATCGTTGCTATTATTGTT+GGG	+	chr8_1:86629602-86629621	None:intergenic	30.0%
	GTCATCTAAGTTTAAGGAAA+TGG	-	chr8_1:86629811-86629830	Msa1222350:CDS	30.0%
	ATCTAAGTTTAAGGAAATGG+TGG	-	chr8_1:86629814-86629833	Msa1222350:CDS	30.0%
	GCTCAATTCAAAAGAGAATT+TGG	-	chr8_1:86630021-86630040	Msa1222350:five_prime_UTR	30.0%
!!!	TGTTTGCTTCTGCGATTTTT+TGG	+	chr8_1:86624914-86624933	None:intergenic	35.0%
!	TAGTATGTCGATTGTTGATC+CGG	+	chr8_1:86625143-86625162	None:intergenic	35.0%
	TCGATATGAAGCTCATTTGT+GGG	-	chr8_1:86625221-86625240	Msa1222350:CDS	35.0%
	ATTTGTGGGACAAGAATTGT+TGG	-	chr8_1:86625235-86625254	Msa1222350:CDS	35.0%
	AATGAGTCTCAGAACAAGAA+AGG	-	chr8_1:86625258-86625277	Msa1222350:CDS	35.0%
	ATGAGTCTCAGAACAAGAAA+GGG	-	chr8_1:86625259-86625278	Msa1222350:CDS	35.0%
	CATGTGGCATTTCAATTCTA+TGG	-	chr8_1:86625370-86625389	Msa1222350:intron	35.0%
!!	AAGCGACACTCATTTTGAAA+CGG	+	chr8_1:86625457-86625476	None:intergenic	35.0%
!	TGTGCAGAGTATTAATTGGA+GGG	+	chr8_1:86625557-86625576	None:intergenic	35.0%
!	GTAGTGTGCAGAGTATTAAT+TGG	+	chr8_1:86625561-86625580	None:intergenic	35.0%
!	TTCAGGTGCTTATGATAATG+AGG	-	chr8_1:86625798-86625817	Msa1222350:intron	35.0%
!	TGCTTATGATAATGAGGAGA+CGG	-	chr8_1:86625804-86625823	Msa1222350:intron	35.0%
!	CTAGCAGCATTGAAATATTG+GGG	-	chr8_1:86625844-86625863	Msa1222350:intron	35.0%
	TTGAAATGGAGAGTCAATCA+AGG	-	chr8_1:86626005-86626024	Msa1222350:intron	35.0%
	TGAAATGGAGAGTCAATCAA+GGG	-	chr8_1:86626006-86626025	Msa1222350:intron	35.0%
!	GAAAAAGCAGTGGTTTTTCA+CGG	-	chr8_1:86626149-86626168	Msa1222350:intron	35.0%
!	AAAAAGCAGTGGTTTTTCAC+GGG	-	chr8_1:86626150-86626169	Msa1222350:intron	35.0%
	ACTACTGTGTTCATATGCAT+TGG	-	chr8_1:86626439-86626458	Msa1222350:intron	35.0%
	CTTGGGACCGAAAATTATAA+AGG	-	chr8_1:86626575-86626594	Msa1222350:intron	35.0%
!!!	TTTTTTACATTGGCCCAAGT+CGG	-	chr8_1:86626644-86626663	Msa1222350:intron	35.0%
	TTGCTTATAATAGTGACCAG+AGG	-	chr8_1:86626946-86626965	Msa1222350:intron	35.0%
	TGCTTATAATAGTGACCAGA+GGG	-	chr8_1:86626947-86626966	Msa1222350:intron	35.0%
	TCAGTTATTACTCCATAGTG+TGG	-	chr8_1:86627444-86627463	Msa1222350:intron	35.0%
	TCACATGTACTCACATTCAA+TGG	-	chr8_1:86627501-86627520	Msa1222350:intron	35.0%
	CACATGTACTCACATTCAAT+GGG	-	chr8_1:86627502-86627521	Msa1222350:intron	35.0%
	ATCTGTGTGAAAAACCAAAC+TGG	+	chr8_1:86627587-86627606	None:intergenic	35.0%
	AGAAAATGGGTCAGATATTC+CGG	-	chr8_1:86627755-86627774	Msa1222350:CDS	35.0%
!	TTCCTTATAATTTGAGACGG+AGG	+	chr8_1:86627851-86627870	None:intergenic	35.0%
	CCGTCCCATATTATAAGAAA+GGG	+	chr8_1:86628134-86628153	None:intergenic	35.0%
	CCCTTTCTTATAATATGGGA+CGG	-	chr8_1:86628131-86628150	Msa1222350:intron	35.0%
	TCCGTCCCATATTATAAGAA+AGG	+	chr8_1:86628135-86628154	None:intergenic	35.0%
	TTTCTTATAATATGGGACGG+AGG	-	chr8_1:86628134-86628153	Msa1222350:intron	35.0%
	TTCTTATAATATGGGACGGA+GGG	-	chr8_1:86628135-86628154	Msa1222350:intron	35.0%
!	CACACCCACTAATTTTTATG+TGG	-	chr8_1:86628197-86628216	Msa1222350:intron	35.0%
	CACTCCCACATAAAAATTAG+TGG	+	chr8_1:86628205-86628224	None:intergenic	35.0%
!	TTTTAGCAAGCTACACCTTA+GGG	-	chr8_1:86628225-86628244	Msa1222350:intron	35.0%
!!!	ACGCCCACTTATTTTTTAGA+AGG	+	chr8_1:86628291-86628310	None:intergenic	35.0%
	AAGTAGGGACTAAACTTTCA+AGG	+	chr8_1:86628527-86628546	None:intergenic	35.0%
	CAACAACAAACAGCTAAATC+TGG	-	chr8_1:86629389-86629408	Msa1222350:intron	35.0%
	ATCTCAGCCGTTATATTAAG+TGG	-	chr8_1:86629570-86629589	Msa1222350:CDS	35.0%
!	GTCATCGTTGCTATTATTGT+TGG	+	chr8_1:86629603-86629622	None:intergenic	35.0%
	TTCATTAGAGTTTGAGTCGT+TGG	+	chr8_1:86629675-86629694	None:intergenic	35.0%
	CTCAAACTCTAATGAAGAAC+TGG	-	chr8_1:86629679-86629698	Msa1222350:intron	35.0%
	TTCTATTGACGAAGCTACAT+TGG	-	chr8_1:86629739-86629758	Msa1222350:CDS	35.0%
	GCTTCAGTCATCTAAGTTTA+AGG	-	chr8_1:86629805-86629824	Msa1222350:CDS	35.0%
!	GTTTAAGGAAATGGTGGAAA+TGG	-	chr8_1:86629820-86629839	Msa1222350:CDS	35.0%
	AAGTACGTTTGAATGTCATC+AGG	+	chr8_1:86629908-86629927	None:intergenic	35.0%
!	ATAATGGAACGTAGGCATAA+TGG	+	chr8_1:86629975-86629994	None:intergenic	35.0%
!	TCAAAGTCATAATGGAACGT+AGG	+	chr8_1:86629983-86630002	None:intergenic	35.0%
	ACGTTCCATTATGACTTTGA+AGG	-	chr8_1:86629983-86630002	Msa1222350:five_prime_UTR	35.0%
!!	CATAGAGCCGATTTTACAGA+CGG	-	chr8_1:86624658-86624677	Msa1222350:three_prime_UTR	40.0%
	TTTGTGGCCGTCTGTAAAAT+CGG	+	chr8_1:86624668-86624687	None:intergenic	40.0%
	ACAAACAACCATCTTTCAGC+CGG	-	chr8_1:86624683-86624702	Msa1222350:three_prime_UTR	40.0%
	GGGGTTAGAAAAATTTGTGC+TGG	+	chr8_1:86624723-86624742	None:intergenic	40.0%
!!	GAATTTAAAGGGATGGGCTA+AGG	+	chr8_1:86624749-86624768	None:intergenic	40.0%
!!!	TTCTGCGATTTTTTGGCCAT+TGG	+	chr8_1:86624907-86624926	None:intergenic	40.0%
	CAATATACAGAGGAGTCACT+AGG	-	chr8_1:86625049-86625068	Msa1222350:CDS	40.0%
	AGAGGAGTCACTAGGTAATT+TGG	-	chr8_1:86625057-86625076	Msa1222350:CDS	40.0%
	TGTTTATCTATCTCTCGTGC+CGG	-	chr8_1:86625121-86625140	Msa1222350:CDS	40.0%
	GTCGATATGAAGCTCATTTG+TGG	-	chr8_1:86625220-86625239	Msa1222350:CDS	40.0%
	GTGGCATTTCAATTCTATGG+TGG	-	chr8_1:86625373-86625392	Msa1222350:intron	40.0%
!!!	GACACTCATTTTGAAACGGA+GGG	+	chr8_1:86625696-86625715	None:intergenic	40.0%
!	GTGTGCAGAGTATTAATTGG+AGG	+	chr8_1:86625558-86625577	None:intergenic	40.0%
!!!	GACACTCATTTTGAAACGGA+GGG	-	chr8_1:86625696-86625715	Msa1222350:intron	40.0%
!	CAATCAAGGGAGGAGTATAT+TGG	-	chr8_1:86626019-86626038	Msa1222350:intron	40.0%
!	AAAAGCAGTGGTTTTTCACG+GGG	-	chr8_1:86626151-86626170	Msa1222350:intron	40.0%
!	CTAAATACAGAGGTGTAGCA+AGG	-	chr8_1:86626179-86626198	Msa1222350:intron	40.0%
	CACTGCAACGTAATTCTAAG+AGG	+	chr8_1:86626392-86626411	None:intergenic	40.0%
	AATTACGTTGCAGTGAACCT+TGG	-	chr8_1:86626397-86626416	Msa1222350:intron	40.0%
	ATTACGTTGCAGTGAACCTT+GGG	-	chr8_1:86626398-86626417	Msa1222350:intron	40.0%
	CTAATTTACGTTGAGTCCCA+AGG	+	chr8_1:86626417-86626436	None:intergenic	40.0%
!	TTTTTACATTGGCCCAAGTC+GGG	-	chr8_1:86626645-86626664	Msa1222350:intron	40.0%
!	CTAGACATCATCATAACGGA+AGG	-	chr8_1:86627018-86627037	Msa1222350:intron	40.0%
!	ACATCATCATAACGGAAGGT+GGG	-	chr8_1:86627022-86627041	Msa1222350:intron	40.0%
	AAGTAGTTAAGTAGCTAGCC+AGG	-	chr8_1:86627288-86627307	Msa1222350:intron	40.0%
!	CAAACTGGCTCAGATAAAAC+GGG	+	chr8_1:86627572-86627591	None:intergenic	40.0%
!	CCAAACTGGCTCAGATAAAA+CGG	+	chr8_1:86627573-86627592	None:intergenic	40.0%
	GAAAATGGGTCAGATATTCC+GGG	-	chr8_1:86627756-86627775	Msa1222350:CDS	40.0%
	AAACATTACTTCTTCCGTCC+CGG	+	chr8_1:86627777-86627796	None:intergenic	40.0%
	CTCCTCCGTCTCAAATTATA+AGG	-	chr8_1:86627846-86627865	Msa1222350:intron	40.0%
!!	GTTTTAGCAAGCTACACCTT+AGG	-	chr8_1:86628224-86628243	Msa1222350:intron	40.0%
	TGTACGTTAGCAACTCCTAT+AGG	+	chr8_1:86628327-86628346	None:intergenic	40.0%
	AAGTAGGGACTAAACTCACA+AGG	-	chr8_1:86628970-86628989	Msa1222350:CDS	40.0%
	ATAACGGCTGAGATCGAAAT+TGG	+	chr8_1:86629564-86629583	None:intergenic	40.0%
	GTGATATTTCATTGTCCACG+AGG	+	chr8_1:86629712-86629731	None:intergenic	40.0%
	TGACTGAAGCAAAAGCTCTA+GGG	+	chr8_1:86629795-86629814	None:intergenic	40.0%
	ATGACTGAAGCAAAAGCTCT+AGG	+	chr8_1:86629796-86629815	None:intergenic	40.0%
	TTCCAATGTGTCAACAACGT+TGG	-	chr8_1:86629853-86629872	Msa1222350:CDS	40.0%
	CAGCACCTTCAAAGTCATAA+TGG	+	chr8_1:86629991-86630010	None:intergenic	40.0%
!!	TTATGACTTTGAAGGTGCTG+AGG	-	chr8_1:86629991-86630010	Msa1222350:five_prime_UTR	40.0%
	GGCTGAAAGATGGTTGTTTG+TGG	+	chr8_1:86624684-86624703	None:intergenic	45.0%
!!	AAAGGGATGGGCTAAGGAAT+GGG	+	chr8_1:86624743-86624762	None:intergenic	45.0%
!!	TAAAGGGATGGGCTAAGGAA+TGG	+	chr8_1:86624744-86624763	None:intergenic	45.0%
	CTGTCACCACCAAAGCAAAA+CGG	-	chr8_1:86624983-86625002	Msa1222350:CDS	45.0%
!	TTTCCTCGTCCGTTTTGCTT+TGG	+	chr8_1:86624995-86625014	None:intergenic	45.0%
	GTTGAGGTGGAGAAGTTCTA+GGG	+	chr8_1:86625023-86625042	None:intergenic	45.0%
	TATATTGAGCTGCGTTGAGG+TGG	+	chr8_1:86625036-86625055	None:intergenic	45.0%
	CTGTATATTGAGCTGCGTTG+AGG	+	chr8_1:86625039-86625058	None:intergenic	45.0%
	CAACGCAGCTCAATATACAG+AGG	-	chr8_1:86625039-86625058	Msa1222350:CDS	45.0%
	CTTCATATCGACCTGTCCAA+CGG	+	chr8_1:86625212-86625231	None:intergenic	45.0%
	CAGAACAAGAAAGGGAGACA+AGG	-	chr8_1:86625267-86625286	Msa1222350:CDS	45.0%
	CTGATTGTTGCAGTCTACCT+TGG	-	chr8_1:86625408-86625427	Msa1222350:intron	45.0%
!!!	CGACACTCATTTTGAAACGG+AGG	+	chr8_1:86625695-86625714	None:intergenic	45.0%
!!!	CGACACTCATTTTGAAACGG+AGG	-	chr8_1:86625695-86625714	Msa1222350:intron	45.0%
	AATGGAGAGTCAATCAAGGG+AGG	-	chr8_1:86626009-86626028	Msa1222350:intron	45.0%
!!	CGGGGAGTATCTAAATACAG+AGG	-	chr8_1:86626169-86626188	Msa1222350:intron	45.0%
!	GACATCATCATAACGGAAGG+TGG	-	chr8_1:86627021-86627040	Msa1222350:intron	45.0%
!!!	GCACGGATTGGCAAAGTTTT+TGG	-	chr8_1:86627047-86627066	Msa1222350:intron	45.0%
!	TCCATAGTGTGGCTTTGCAT+GGG	-	chr8_1:86627455-86627474	Msa1222350:intron	45.0%
!	GGCTTTGCATGGGTTATGTA+TGG	-	chr8_1:86627465-86627484	Msa1222350:intron	45.0%
!	CCGTTTTATCTGAGCCAGTT+TGG	-	chr8_1:86627570-86627589	Msa1222350:intron	45.0%
	AGCTGTAGCTGCTTCTTCTT+GGG	+	chr8_1:86629498-86629517	None:intergenic	45.0%
	AAGCTGTAGCTGCTTCTTCT+TGG	+	chr8_1:86629499-86629518	None:intergenic	45.0%
	AGCAGCTACAGCTTATGACA+TGG	-	chr8_1:86629505-86629524	Msa1222350:intron	45.0%
	AGAACTGGAATTTAGCCTCG+TGG	-	chr8_1:86629694-86629713	Msa1222350:intron	45.0%
	CTCCAACGTTGTTGACACAT+TGG	+	chr8_1:86629858-86629877	None:intergenic	45.0%
!	ACGTAGGCATAATGGAGTTG+AGG	+	chr8_1:86629967-86629986	None:intergenic	45.0%
!!	ATATTTATATATAGTATTAA+TGG	+	chr8_1:86624840-86624859	None:intergenic	5.0%
!!!	ATAATAAGATAATAATTTTT+CGG	+	chr8_1:86626607-86626626	None:intergenic	5.0%
!!!	TATAAGTAATTTTTTTTTAT+AGG	-	chr8_1:86628925-86628944	Msa1222350:CDS	5.0%
!!!	ATAAGTAATTTTTTTTTATA+GGG	-	chr8_1:86628926-86628945	Msa1222350:CDS	5.0%
!!!	AATTAAATTTTTATATAACA+CGG	+	chr8_1:86629265-86629284	None:intergenic	5.0%
	TGTGATAGCCGGCTGAAAGA+TGG	+	chr8_1:86624694-86624713	None:intergenic	50.0%
	GCTGGCAGAATTGTGATAGC+CGG	+	chr8_1:86624705-86624724	None:intergenic	50.0%
!!	AAGGGATGGGCTAAGGAATG+GGG	+	chr8_1:86624742-86624761	None:intergenic	50.0%
	CGTTGAGGTGGAGAAGTTCT+AGG	+	chr8_1:86625024-86625043	None:intergenic	50.0%
	AATTTGTCCGGTCCCGACTT+GGG	+	chr8_1:86626660-86626679	None:intergenic	50.0%
	AAATTTGTCCGGTCCCGACT+TGG	+	chr8_1:86626661-86626680	None:intergenic	50.0%
!	TCATCATAACGGAAGGTGGG+AGG	-	chr8_1:86627025-86627044	Msa1222350:intron	50.0%
!	CTCCATAGTGTGGCTTTGCA+TGG	-	chr8_1:86627454-86627473	Msa1222350:intron	50.0%
	ACCCATGCAAAGCCACACTA+TGG	+	chr8_1:86627459-86627478	None:intergenic	50.0%
	GGACGGAGGGAGTAGTTTAT+TGG	+	chr8_1:86627551-86627570	None:intergenic	50.0%
	CTCAGATAAAACGGGACGGA+GGG	+	chr8_1:86627564-86627583	None:intergenic	50.0%
	CTGGCTCAGATAAAACGGGA+CGG	+	chr8_1:86627568-86627587	None:intergenic	50.0%
	ATGGGTCAGATATTCCGGGA+CGG	-	chr8_1:86627760-86627779	Msa1222350:CDS	50.0%
!!	CAAAAGCTCTAGGGCTGATG+TGG	+	chr8_1:86629786-86629805	None:intergenic	50.0%
	GGAGTTGAGGTCTTCCAACA+TGG	+	chr8_1:86629954-86629973	None:intergenic	50.0%
!	CCTCGTCCGTTTTGCTTTGG+TGG	+	chr8_1:86624992-86625011	None:intergenic	55.0%
	CCACCAAAGCAAAACGGACG+AGG	-	chr8_1:86624989-86625008	Msa1222350:CDS	55.0%
!	TACCATCGATCAGGCACCGT+TGG	-	chr8_1:86625193-86625212	Msa1222350:CDS	55.0%
!	ATAACGGAAGGTGGGAGGCA+CGG	-	chr8_1:86627030-86627049	Msa1222350:intron	55.0%
	GCTCAGATAAAACGGGACGG+AGG	+	chr8_1:86627565-86627584	None:intergenic	55.0%
	GATGTGGCACTAGTTGGTCG+AGG	+	chr8_1:86629770-86629789	None:intergenic	55.0%
	AGGGCTGATGTGGCACTAGT+TGG	+	chr8_1:86629776-86629795	None:intergenic	55.0%
	GTCCAACGGTGCCTGATCGA+TGG	+	chr8_1:86625198-86625217	None:intergenic	60.0%
!	TCGATCAGGCACCGTTGGAC+AGG	-	chr8_1:86625198-86625217	Msa1222350:CDS	60.0%
	ACATTGGCCCAAGTCGGGAC+CGG	-	chr8_1:86626650-86626669	Msa1222350:intron	60.0%
!	GGAAGGTGGGAGGCACGGAT+TGG	-	chr8_1:86627035-86627054	Msa1222350:intron	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr8_1	gene	86624542	86630262	86624542	ID=Msa1222350;Name=Msa1222350
chr8_1	mRNA	86624542	86630262	86624542	ID=Msa1222350-mRNA-1;Parent=Msa1222350;Name=Msa1222350-mRNA-1;_AED=0.11;_eAED=0.11;_QI=365\|0.83\|1\|1\|1\|1\|7\|241\|355
chr8_1	exon	86624542	86625312	86624542	ID=Msa1222350-mRNA-1:exon:31193;Parent=Msa1222350-mRNA-1
chr8_1	exon	86627706	86627782	86627706	ID=Msa1222350-mRNA-1:exon:31192;Parent=Msa1222350-mRNA-1
chr8_1	exon	86628604	86628654	86628604	ID=Msa1222350-mRNA-1:exon:31191;Parent=Msa1222350-mRNA-1
chr8_1	exon	86628751	86628824	86628751	ID=Msa1222350-mRNA-1:exon:31190;Parent=Msa1222350-mRNA-1
chr8_1	exon	86628913	86629001	86628913	ID=Msa1222350-mRNA-1:exon:31189;Parent=Msa1222350-mRNA-1
chr8_1	exon	86629516	86629598	86629516	ID=Msa1222350-mRNA-1:exon:31188;Parent=Msa1222350-mRNA-1
chr8_1	exon	86629734	86630262	86629734	ID=Msa1222350-mRNA-1:exon:31187;Parent=Msa1222350-mRNA-1
chr8_1	five_prime_UTR	86629898	86630262	86629898	ID=Msa1222350-mRNA-1:five_prime_utr;Parent=Msa1222350-mRNA-1
chr8_1	CDS	86629734	86629897	86629734	ID=Msa1222350-mRNA-1:cds;Parent=Msa1222350-mRNA-1
chr8_1	CDS	86629516	86629598	86629516	ID=Msa1222350-mRNA-1:cds;Parent=Msa1222350-mRNA-1
chr8_1	CDS	86628913	86629001	86628913	ID=Msa1222350-mRNA-1:cds;Parent=Msa1222350-mRNA-1
chr8_1	CDS	86628751	86628824	86628751	ID=Msa1222350-mRNA-1:cds;Parent=Msa1222350-mRNA-1
chr8_1	CDS	86628604	86628654	86628604	ID=Msa1222350-mRNA-1:cds;Parent=Msa1222350-mRNA-1
chr8_1	CDS	86627706	86627782	86627706	ID=Msa1222350-mRNA-1:cds;Parent=Msa1222350-mRNA-1
chr8_1	CDS	86624783	86625312	86624783	ID=Msa1222350-mRNA-1:cds;Parent=Msa1222350-mRNA-1
chr8_1	three_prime_UTR	86624542	86624782	86624542	ID=Msa1222350-mRNA-1:three_prime_utr;Parent=Msa1222350-mRNA-1

Gene Sequence

>Msa1222350

ATGGCCAAAAAATCGCAGAAGCAAACAGAGAAAGATAATGCAAGCAATGATAATAATAATCTGAATGCAAGTAACACTGTCACCACCAAAGCAAAACGGACGAGGAAAAGTGTCCCTAGAACTTCTCCACCTCAACGCAGCTCAATATACAGAGGAGTCACTAGGCACCGTTGGACAGGTCGATATGAAGCTCATTTGTGGGACAAGAATTGTTGGAATGAGTCTCAGAACAAGAAAGGGAGACAAGGTGCTTATGATAATGAGGAGACGGCAGCACATGCTTATGATCTAGCAGCATTGAAATATTGGGGTCAAGATACCATTATTAATTTTCCGCTATCAAACTATCAAAAAGAACTAATTGAAATGGAGAGTCAATCAAGGGAGGAGTATATTGGATCCTTAAGAAGAAAAAGCAGTGGTTTTTCACGGGGAGTATCTAAATACAGAGGTGTAGCAAGACATCATCATAACGGAAGGTGGGAGGCACGGATTGGCAAAGTTTTTGGCAATAAGTATCTTTATCTCGGAACATATGCTACCCAAGAAGAAGCAGCTACAGCTTATGACATGGCAGCTATTGAATACCGTGGACTTAATGCTGTTACCAATTTCGATCTCAGCCGTTATATTAAGTGGCTTAAACCCAACAATAATAGCAACGATGACAATAACAAGTCCAATATTAATCTTTGTGACATTAACTCTAACAGTAGTGCCAACGACTCAAACTCTAATGAAGAACTGGAATTTAGCCTCGTGGACAATGAAATATCACTCAATAATTCTATTGACGAAGCTACATTGGTTCAACCTCGACCAACTAGTGCCACATCAGCCCTAGAGCTTTTGCTTCAGTCATCTAAGTTTAAGGAAATGGTGGAAATGGCATCAATGACTTCCAATGTGTCAACAACGTTGGAGTCTGATCAGTTGTCACAATGTGCATTTCCTGATGACATTCAAACGTACTTTGAATATGAAAATTTCAATGATACCATGTTGGAAGACCTCAACTCCATTATGCCTACGTTCCATTATGACTTTGAAGGTGCTGAGGTTTTATAG

Protein sequence

>Msa1222350

MAKKSQKQTEKDNASNDNNNLNASNTVTTKAKRTRKSVPRTSPPQRSSIYRGVTRHRWTGRYEAHLWDKNCWNESQNKKGRQGAYDNEETAAHAYDLAALKYWGQDTIINFPLSNYQKELIEMESQSREEYIGSLRRKSSGFSRGVSKYRGVARHHHNGRWEARIGKVFGNKYLYLGTYATQEEAATAYDMAAIEYRGLNAVTNFDLSRYIKWLKPNNNSNDDNNKSNINLCDINSNSSANDSNSNEELEFSLVDNEISLNNSIDEATLVQPRPTSATSALELLLQSSKFKEMVEMASMTSNVSTTLESDQLSQCAFPDDIQTYFEYENFNDTMLEDLNSIMPTFHYDFEGAEVL*