AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1236710


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1236710 MtrunA17_Chr8g0346911 95.918 98 3 1 1 98 1 97 6.90e-61 185
Msa1236710 MtrunA17_Chr3g0126811 64.407 59 21 0 40 98 17 75 1.79e-19 76.6
Msa1236710 MtrunA17_Chr1g0151621 52.459 61 29 0 38 98 53 113 4.56e-14 65.1
Msa1236710 MtrunA17_Chr8g0388331 57.143 56 24 0 43 98 19 74 6.10e-14 64.3
Msa1236710 MtrunA17_Chr4g0055421 52.542 59 28 0 40 98 16 74 7.06e-14 64.3
Msa1236710 MtrunA17_Chr5g0446931 50.847 59 29 0 40 98 31 89 2.75e-13 62.8
Msa1236710 MtrunA17_Chr3g0102821 49.153 59 30 0 40 98 31 89 5.93e-13 62.0
Msa1236710 MtrunA17_Chr5g0399921 49.153 59 30 0 40 98 15 73 1.90e-12 58.5
Msa1236710 MtrunA17_Chr4g0076551 49.091 55 28 0 44 98 36 90 2.45e-12 60.1
Msa1236710 MtrunA17_Chr8g0341941 53.704 54 25 0 45 98 66 119 3.58e-12 60.1
Msa1236710 MtrunA17_Chr2g0290771 55.319 47 21 0 52 98 8 54 1.83e-11 57.4
Msa1236710 MtrunA17_Chr2g0290751 54.000 50 23 0 49 98 43 92 2.24e-11 57.4
Msa1236710 MtrunA17_Chr2g0290761 54.000 50 23 0 49 98 43 92 2.93e-11 57.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1236710 AT5G24270.4 47.959 98 31 1 1 98 1 78 4.07e-21 83.6
Msa1236710 AT5G24270.1 47.959 98 31 1 1 98 1 78 4.07e-21 83.6
Msa1236710 AT5G24270.2 47.959 98 31 1 1 98 1 78 4.07e-21 83.6
Msa1236710 AT1G64480.4 61.017 59 23 0 40 98 20 78 6.98e-18 74.7
Msa1236710 AT1G64480.2 61.017 59 23 0 40 98 20 78 9.06e-18 74.7
Msa1236710 AT1G64480.3 61.017 59 23 0 40 98 20 78 9.06e-18 74.7
Msa1236710 AT1G64480.1 61.017 59 23 0 40 98 20 78 9.06e-18 74.7
Msa1236710 AT5G24270.5 64.000 50 18 0 49 98 4 53 8.10e-15 66.6
Msa1236710 AT5G47100.2 57.407 54 23 0 45 98 21 74 5.27e-14 64.3
Msa1236710 AT5G47100.1 57.407 54 23 0 45 98 21 74 6.83e-14 64.7
Msa1236710 AT4G17615.4 51.852 54 26 0 45 98 21 74 3.34e-12 60.1
Msa1236710 AT4G17615.1 51.852 54 26 0 45 98 21 74 3.34e-12 60.1
Msa1236710 AT4G17615.5 51.852 54 26 0 45 98 21 74 3.34e-12 60.1
Msa1236710 AT4G26570.2 48.276 58 30 0 41 98 32 89 3.70e-12 60.1
Msa1236710 AT4G26570.1 48.276 58 30 0 41 98 32 89 3.86e-12 60.1
Msa1236710 AT4G33000.3 53.448 58 27 0 41 98 53 110 4.32e-12 58.9
Msa1236710 AT4G33000.4 53.448 58 27 0 41 98 53 110 6.99e-12 58.9
Msa1236710 AT4G33000.6 53.448 58 27 0 41 98 53 110 6.99e-12 58.9
Msa1236710 AT4G17615.3 50.847 59 29 0 40 98 16 74 7.29e-12 59.7
Msa1236710 AT5G55990.1 46.552 58 31 0 41 98 32 89 8.99e-12 58.9
Msa1236710 AT5G55990.2 46.552 58 31 0 41 98 32 89 8.99e-12 58.9
Msa1236710 AT4G33000.5 53.448 58 27 0 41 98 53 110 1.17e-11 58.5
Msa1236710 AT4G33000.2 53.448 58 27 0 41 98 53 110 1.18e-11 58.9
Msa1236710 AT4G33000.1 53.448 58 27 0 41 98 63 120 1.42e-11 58.9

Find 18 sgRNAs with CRISPR-Local

Find 99 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGCATATATATTAAATTAA+TGG 0.254151 8_2:-15873023 None:intergenic
AAAAGTAGCAGAAAATATTA+TGG 0.327214 8_2:+15873259 Msa1236710:CDS
CATAAAGTTTATGTAAAGAA+TGG 0.346436 8_2:+15873223 Msa1236710:exon
GAGAAATTTATATGCCTTCA+CGG 0.403990 8_2:-15872984 None:intergenic
AAACAAACGCAACCTCTTTG+TGG 0.437485 8_2:+15874239 Msa1236710:CDS
GACATAAGCTTAATAAGTGT+TGG 0.437812 8_2:-15873135 None:intergenic
ATAAAGTTTATGTAAAGAAT+GGG 0.465377 8_2:+15873224 Msa1236710:exon
GAGAGAGGTGGAAATCTTGA+AGG 0.495718 8_2:+15873992 Msa1236710:CDS
CTTATCTTTGCCAACTTCAG+AGG 0.524395 8_2:-15873288 None:intergenic
ACGCAACCTCTTTGTGGATA+GGG 0.528181 8_2:+15874245 Msa1236710:CDS
TACATGGTGTTTCAGAAGCA+AGG 0.531935 8_2:-15873373 None:intergenic
ATGGATGGATCCTCTGAAGT+TGG 0.534150 8_2:+15873278 Msa1236710:CDS
GTAGCAGAAAATATTATGGA+TGG 0.537395 8_2:+15873263 Msa1236710:CDS
AACGCAACCTCTTTGTGGAT+AGG 0.564160 8_2:+15874244 Msa1236710:CDS
CCTAGCAGTTACTGTGAGAG+AGG 0.600150 8_2:+15873977 Msa1236710:intron
TGCATACCCTATCCACAAAG+AGG 0.624228 8_2:-15874251 None:intergenic
TAATCTATTAGGAACCGTGA+AGG 0.651647 8_2:+15872970 Msa1236710:five_prime_UTR
AGCAGTTACTGTGAGAGAGG+TGG 0.654761 8_2:+15873980 Msa1236710:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATGCATATATATTAAATTAA+TGG - chr8_2:15873026-15873045 None:intergenic 10.0%
!! ATGCATATATATTAAATTAA+TGG - chr8_2:15873026-15873045 None:intergenic 10.0%
!! ATAAAGTTTATGTAAAGAAT+GGG + chr8_2:15873224-15873243 Msa1236710:exon 15.0%
!! GAAAAAAAAAAAACTTGAAA+AGG - chr8_2:15873412-15873431 None:intergenic 15.0%
!! CTTATAATCTGTAAAATTAT+AGG + chr8_2:15873468-15873487 Msa1236710:intron 15.0%
!! ATATATTAAGTGTAAAAAAG+AGG - chr8_2:15873565-15873584 None:intergenic 15.0%
!! AATTAGTTATTGCTTAAAAA+AGG + chr8_2:15873586-15873605 Msa1236710:intron 15.0%
!! ATTAGTTATTGCTTAAAAAA+GGG + chr8_2:15873587-15873606 Msa1236710:intron 15.0%
!! ATAAAGTTTATGTAAAGAAT+GGG + chr8_2:15873224-15873243 Msa1236710:exon 15.0%
!! GAAAAAAAAAAAACTTGAAA+AGG - chr8_2:15873412-15873431 None:intergenic 15.0%
!! CTTATAATCTGTAAAATTAT+AGG + chr8_2:15873468-15873487 Msa1236710:intron 15.0%
!! ATATATTAAGTGTAAAAAAG+AGG - chr8_2:15873565-15873584 None:intergenic 15.0%
!! AATTAGTTATTGCTTAAAAA+AGG + chr8_2:15873586-15873605 Msa1236710:intron 15.0%
!! ATTAGTTATTGCTTAAAAAA+GGG + chr8_2:15873587-15873606 Msa1236710:intron 15.0%
!!! AAAATTGACTTTTCTTTCAT+TGG + chr8_2:15873109-15873128 Msa1236710:five_prime_UTR 20.0%
!! CATAAAGTTTATGTAAAGAA+TGG + chr8_2:15873223-15873242 Msa1236710:exon 20.0%
!! AAAAGTAGCAGAAAATATTA+TGG + chr8_2:15873259-15873278 Msa1236710:CDS 20.0%
!!! TCAAGTTTTTTTTTTTTCCT+TGG + chr8_2:15873414-15873433 Msa1236710:intron 20.0%
!!! AATATACTACTTTGATTTAG+TGG + chr8_2:15873532-15873551 Msa1236710:intron 20.0%
!! TTAGTTATTGCTTAAAAAAG+GGG + chr8_2:15873588-15873607 Msa1236710:intron 20.0%
!!! AAAATTGACTTTTCTTTCAT+TGG + chr8_2:15873109-15873128 Msa1236710:five_prime_UTR 20.0%
!! CATAAAGTTTATGTAAAGAA+TGG + chr8_2:15873223-15873242 Msa1236710:exon 20.0%
!! AAAAGTAGCAGAAAATATTA+TGG + chr8_2:15873259-15873278 Msa1236710:CDS 20.0%
!!! TCAAGTTTTTTTTTTTTCCT+TGG + chr8_2:15873414-15873433 Msa1236710:intron 20.0%
!!! AATATACTACTTTGATTTAG+TGG + chr8_2:15873532-15873551 Msa1236710:intron 20.0%
!! TTAGTTATTGCTTAAAAAAG+GGG + chr8_2:15873588-15873607 Msa1236710:intron 20.0%
! AGTCAAAAACGTAATCTATT+AGG + chr8_2:15872959-15872978 Msa1236710:five_prime_UTR 25.0%
!! TCATTTTCTTTTACTTGTGA+AGG - chr8_2:15873320-15873339 None:intergenic 25.0%
! ACGTGATTTAGATAATTTCA+TGG + chr8_2:15873496-15873515 Msa1236710:intron 25.0%
!! AAATAAGTTTAAAATGCTCG+CGG - chr8_2:15873705-15873724 None:intergenic 25.0%
!!! CTTATTTTCATAAGTTCTAC+AGG + chr8_2:15873718-15873737 Msa1236710:intron 25.0%
! TAGCTTATGAAAACAGATTA+TGG + chr8_2:15873742-15873761 Msa1236710:intron 25.0%
! CATAAATTAAAACACACAAG+TGG - chr8_2:15873906-15873925 None:intergenic 25.0%
!! GAAAAATGGACTTATTCAAA+AGG + chr8_2:15874046-15874065 Msa1236710:CDS 25.0%
! TGTGTATTGTGTATATTAAC+AGG + chr8_2:15874185-15874204 Msa1236710:intron 25.0%
! AGTCAAAAACGTAATCTATT+AGG + chr8_2:15872959-15872978 Msa1236710:five_prime_UTR 25.0%
!! TCATTTTCTTTTACTTGTGA+AGG - chr8_2:15873320-15873339 None:intergenic 25.0%
! ACGTGATTTAGATAATTTCA+TGG + chr8_2:15873496-15873515 Msa1236710:intron 25.0%
!! AAATAAGTTTAAAATGCTCG+CGG - chr8_2:15873705-15873724 None:intergenic 25.0%
!!! CTTATTTTCATAAGTTCTAC+AGG + chr8_2:15873718-15873737 Msa1236710:intron 25.0%
! TAGCTTATGAAAACAGATTA+TGG + chr8_2:15873742-15873761 Msa1236710:intron 25.0%
! CATAAATTAAAACACACAAG+TGG - chr8_2:15873906-15873925 None:intergenic 25.0%
!! GAAAAATGGACTTATTCAAA+AGG + chr8_2:15874046-15874065 Msa1236710:CDS 25.0%
! TGTGTATTGTGTATATTAAC+AGG + chr8_2:15874185-15874204 Msa1236710:intron 25.0%
GAGAAATTTATATGCCTTCA+CGG - chr8_2:15872987-15873006 None:intergenic 30.0%
GACATAAGCTTAATAAGTGT+TGG - chr8_2:15873138-15873157 None:intergenic 30.0%
GTAGCAGAAAATATTATGGA+TGG + chr8_2:15873263-15873282 Msa1236710:CDS 30.0%
! CAAGACAATTTAACTTTCCA+AGG - chr8_2:15873434-15873453 None:intergenic 30.0%
ATAATCACTTCTGCTACAAT+CGG + chr8_2:15873836-15873855 Msa1236710:intron 30.0%
ATTAAAACACACAAGTGGAA+AGG - chr8_2:15873901-15873920 None:intergenic 30.0%
GTACACTATTCATATGCAAT+TGG - chr8_2:15873932-15873951 None:intergenic 30.0%
TGCACAGTTATTGTGAAAAA+TGG + chr8_2:15874032-15874051 Msa1236710:CDS 30.0%
GAGAAATTTATATGCCTTCA+CGG - chr8_2:15872987-15873006 None:intergenic 30.0%
GACATAAGCTTAATAAGTGT+TGG - chr8_2:15873138-15873157 None:intergenic 30.0%
GTAGCAGAAAATATTATGGA+TGG + chr8_2:15873263-15873282 Msa1236710:CDS 30.0%
! CAAGACAATTTAACTTTCCA+AGG - chr8_2:15873434-15873453 None:intergenic 30.0%
ATAATCACTTCTGCTACAAT+CGG + chr8_2:15873836-15873855 Msa1236710:intron 30.0%
ATTAAAACACACAAGTGGAA+AGG - chr8_2:15873901-15873920 None:intergenic 30.0%
GTACACTATTCATATGCAAT+TGG - chr8_2:15873932-15873951 None:intergenic 30.0%
TGCACAGTTATTGTGAAAAA+TGG + chr8_2:15874032-15874051 Msa1236710:CDS 30.0%
TAATCTATTAGGAACCGTGA+AGG + chr8_2:15872970-15872989 Msa1236710:five_prime_UTR 35.0%
AGGAAGCTTTCTTACTTACA+TGG - chr8_2:15873392-15873411 None:intergenic 35.0%
CATAGTGCATACCATCTTAA+GGG + chr8_2:15873614-15873633 Msa1236710:intron 35.0%
! ATACCATCTTAAGGGCTTTT+TGG + chr8_2:15873622-15873641 Msa1236710:intron 35.0%
AATCCAAAAAGCCCTTAAGA+TGG - chr8_2:15873628-15873647 None:intergenic 35.0%
! TTTGGATTGACTTCTACACT+TGG + chr8_2:15873640-15873659 Msa1236710:intron 35.0%
ATCCAAACAGAACCTGAAAT+TGG + chr8_2:15873873-15873892 Msa1236710:intron 35.0%
!! AACCAATTTCAGGTTCTGTT+TGG - chr8_2:15873878-15873897 None:intergenic 35.0%
ACTTGAATCTCTTGTTCGAT+TGG + chr8_2:15874100-15874119 Msa1236710:intron 35.0%
TAATCTATTAGGAACCGTGA+AGG + chr8_2:15872970-15872989 Msa1236710:five_prime_UTR 35.0%
AGGAAGCTTTCTTACTTACA+TGG - chr8_2:15873392-15873411 None:intergenic 35.0%
CATAGTGCATACCATCTTAA+GGG + chr8_2:15873614-15873633 Msa1236710:intron 35.0%
! ATACCATCTTAAGGGCTTTT+TGG + chr8_2:15873622-15873641 Msa1236710:intron 35.0%
AATCCAAAAAGCCCTTAAGA+TGG - chr8_2:15873628-15873647 None:intergenic 35.0%
! TTTGGATTGACTTCTACACT+TGG + chr8_2:15873640-15873659 Msa1236710:intron 35.0%
ATCCAAACAGAACCTGAAAT+TGG + chr8_2:15873873-15873892 Msa1236710:intron 35.0%
!! AACCAATTTCAGGTTCTGTT+TGG - chr8_2:15873878-15873897 None:intergenic 35.0%
ACTTGAATCTCTTGTTCGAT+TGG + chr8_2:15874100-15874119 Msa1236710:intron 35.0%
CTTATCTTTGCCAACTTCAG+AGG - chr8_2:15873291-15873310 None:intergenic 40.0%
TACATGGTGTTTCAGAAGCA+AGG - chr8_2:15873376-15873395 None:intergenic 40.0%
GCATAGTGCATACCATCTTA+AGG + chr8_2:15873613-15873632 Msa1236710:intron 40.0%
AGTGGAAAGGAACCAATTTC+AGG - chr8_2:15873888-15873907 None:intergenic 40.0%
AAACAAACGCAACCTCTTTG+TGG + chr8_2:15874239-15874258 Msa1236710:CDS 40.0%
CTTATCTTTGCCAACTTCAG+AGG - chr8_2:15873291-15873310 None:intergenic 40.0%
TACATGGTGTTTCAGAAGCA+AGG - chr8_2:15873376-15873395 None:intergenic 40.0%
GCATAGTGCATACCATCTTA+AGG + chr8_2:15873613-15873632 Msa1236710:intron 40.0%
AGTGGAAAGGAACCAATTTC+AGG - chr8_2:15873888-15873907 None:intergenic 40.0%
AAACAAACGCAACCTCTTTG+TGG + chr8_2:15874239-15874258 Msa1236710:CDS 40.0%
!! ATGGATGGATCCTCTGAAGT+TGG + chr8_2:15873278-15873297 Msa1236710:CDS 45.0%
GAGAGAGGTGGAAATCTTGA+AGG + chr8_2:15873992-15874011 Msa1236710:CDS 45.0%
AACGCAACCTCTTTGTGGAT+AGG + chr8_2:15874244-15874263 Msa1236710:CDS 45.0%
!! ATGGATGGATCCTCTGAAGT+TGG + chr8_2:15873278-15873297 Msa1236710:CDS 45.0%
GAGAGAGGTGGAAATCTTGA+AGG + chr8_2:15873992-15874011 Msa1236710:CDS 45.0%
CCTCTCTCACAGTAACTGCT+AGG - chr8_2:15873980-15873999 None:intergenic 50.0%
CCTAGCAGTTACTGTGAGAG+AGG + chr8_2:15873977-15873996 Msa1236710:intron 50.0%
AGCAGTTACTGTGAGAGAGG+TGG + chr8_2:15873980-15873999 Msa1236710:intron 50.0%
CCTCTCTCACAGTAACTGCT+AGG - chr8_2:15873980-15873999 None:intergenic 50.0%
CCTAGCAGTTACTGTGAGAG+AGG + chr8_2:15873977-15873996 Msa1236710:intron 50.0%
AGCAGTTACTGTGAGAGAGG+TGG + chr8_2:15873980-15873999 Msa1236710:intron 50.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 15872939 15874266 15872939 ID=Msa1236710;Name=Msa1236710
chr8_2 mRNA 15872939 15874266 15872939 ID=Msa1236710-mRNA-1;Parent=Msa1236710;Name=Msa1236710-mRNA-1;_AED=0.07;_eAED=0.07;_QI=303|0.5|0.33|0.66|1|1|3|0|98
chr8_2 exon 15872939 15873392 15872939 ID=Msa1236710-mRNA-1:exon:5939;Parent=Msa1236710-mRNA-1
chr8_2 exon 15873985 15874067 15873985 ID=Msa1236710-mRNA-1:exon:5940;Parent=Msa1236710-mRNA-1
chr8_2 exon 15874207 15874266 15874207 ID=Msa1236710-mRNA-1:exon:5941;Parent=Msa1236710-mRNA-1
chr8_2 five_prime_UTR 15872939 15873241 15872939 ID=Msa1236710-mRNA-1:five_prime_utr;Parent=Msa1236710-mRNA-1
chr8_2 CDS 15873242 15873392 15873242 ID=Msa1236710-mRNA-1:cds;Parent=Msa1236710-mRNA-1
chr8_2 CDS 15873985 15874067 15873985 ID=Msa1236710-mRNA-1:cds;Parent=Msa1236710-mRNA-1
chr8_2 CDS 15874207 15874266 15874207 ID=Msa1236710-mRNA-1:cds;Parent=Msa1236710-mRNA-1
Gene Sequence

>Msa1236710

ATGGGTTGCAGTAGCTCAAAAGTAGCAGAAAATATTATGGATGGATCCTCTGAAGTTGGCAAAGATAAGAATCTACCTTCACAAGTAAAAGAAAATGAGCTTAATAATTCTTGTTCTGAAGATTTTTCTATCCTTGCTTCTGAAACACCATTTACTGTGAGAGAGGTGGAAATCTTGAAGGAGTTGTACAAAAAATTATGCACAGTTATTGTGAAAAATGGACTTATTCAAAAGGAAGAGCTTCACTTTGCTCTTTTTAGAAACAGAAACAAACGCAACCTCTTTGTGGATAGG

Protein sequence

>Msa1236710

MGCSSSKVAENIMDGSSEVGKDKNLPSQVKENELNNSCSEDFSILASETPFTVREVEILKELYKKLCTVIVKNGLIQKEELHFALFRNRNKRNLFVDR