AlfalfaGEDB | Msa0015480

M. sativa cultivar zhongmu-4 / Msa0015480

KEGG: K19704
GO: -
PFAM: PF00481.24

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0015480	sp\|Q9LW60\|P2C44_ARATH	43.885	278	132	6	1	273	1	259	3.14e-70	220

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0007440	Msa0015480	0.812500	4.109969e-51	-8.615850e-47
Msa0007470	Msa0015480	0.805991	1.021737e-49	-8.615850e-47
Msa0015460	Msa0015480	0.949996	4.090380e-108	-8.615850e-47
Msa0015480	Msa0037540	-0.845621	3.621851e-59	-8.615850e-47
Msa0015480	Msa0037590	-0.843584	1.280709e-58	-8.615850e-47
Msa0015480	Msa0054260	0.804204	2.417148e-49	-8.615850e-47
Msa0015480	Msa0064700	0.853992	1.655244e-61	-8.615850e-47
Msa0015480	Msa0065010	0.809482	1.851517e-50	-8.615850e-47
Msa0015480	Msa0082680	-0.809871	1.527516e-50	-8.615850e-47
Msa0015480	Msa0091250	-0.822370	2.456074e-53	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0015480	MtrunA17_Chr1g0166481	97.080	274	8	0	1	274	1	274	0.0	552
Msa0015480	MtrunA17_Chr1g0187481	60.432	278	106	1	1	274	1	278	8.79e-120	343
Msa0015480	MtrunA17_Chr8g0360351	37.891	256	129	6	41	273	33	281	4.93e-39	137
Msa0015480	MtrunA17_Chr2g0279151	34.457	267	145	5	30	273	21	280	2.15e-34	125
Msa0015480	MtrunA17_Chr4g0050381	32.143	280	160	5	17	273	4	276	4.93e-34	124
Msa0015480	MtrunA17_Chr2g0317021	36.328	256	133	6	41	273	84	332	6.21e-33	123
Msa0015480	MtrunA17_Chr5g0426141	35.398	226	121	4	71	273	58	281	4.77e-32	119
Msa0015480	MtrunA17_Chr3g0115851	32.743	226	127	4	71	273	58	281	6.20e-28	108
Msa0015480	MtrunA17_Chr4g0051661	32.031	256	143	5	41	273	150	397	4.56e-25	102
Msa0015480	MtrunA17_Chr1g0150451	28.837	215	132	3	80	273	109	323	2.01e-24	100

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0015480	AT3G23360.1	43.885	278	132	6	1	273	1	259	3.20e-71	220
Msa0015480	AT4G28400.3	35.938	256	134	5	41	273	12	260	7.24e-38	134
Msa0015480	AT4G28400.1	35.938	256	134	5	41	273	34	282	1.16e-37	134
Msa0015480	AT2G20630.1	34.375	256	138	5	41	273	30	278	1.50e-37	134
Msa0015480	AT2G20630.2	33.984	256	139	5	41	273	30	278	3.76e-35	128
Msa0015480	AT3G15260.2	33.574	277	153	7	20	273	20	288	1.54e-34	126
Msa0015480	AT3G15260.1	33.574	277	153	7	20	273	20	288	1.54e-34	126
Msa0015480	AT1G22280.1	34.222	225	123	4	72	273	58	280	6.56e-30	114
Msa0015480	AT1G22280.3	33.766	231	122	5	72	273	58	286	3.67e-29	112
Msa0015480	AT1G78200.1	34.274	248	135	6	52	273	37	282	1.31e-28	110

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Find 51 sgRNAs with CRISPR-Local

Find 228 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACACTCTGCTGCTTCTTG+TGG	0.521856	1_1:-25016773	Msa0015470:intron
AAACTCGTGCTAGCCAACAT+CGG	0.656947	1_1:+25015773	Msa0015480:CDS
AAAGCTTCAGAACTTGTGGT+TGG	0.510242	1_1:+25016466	Msa0015480:CDS
AAATTGGTGAGACAGAATTG+TGG	0.696148	1_1:+25014989	Msa0015480:CDS
AACTTGTGGTTGGAAGTTAT+TGG	0.296396	1_1:+25016476	Msa0015480:CDS
AAGAAGCAGTGAATCTTATC+AGG	0.393702	1_1:+25016738	Msa0015480:CDS
AATATCACATGGTCATCATG+TGG	0.739236	1_1:+25014897	Msa0015480:CDS
AATTATGTTAGTCTCATTTG+AGG	0.462042	1_1:+25015627	Msa0015480:intron
ACAAGAAGCAGCAGAGTGTT+TGG	0.405610	1_1:+25016775	Msa0015480:CDS
ACACTCAAGAGAGCATACCT+AGG	0.689445	1_1:+25015665	Msa0015480:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CTTCATGTAACAATGGAATA+AGG	-	chr1_1:25014665-25014684	Msa0015470:intron	30.0%
CTTATTCCATTGTTACATGA+AGG	+	chr1_1:25014663-25014682	Msa0015480:intron	30.0%
CTCATGTTTAGGAAATAGAA+AGG	+	chr1_1:25014828-25014847	Msa0015480:CDS	30.0%
AGAAAGGTAAGAAGAAATCA+TGG	+	chr1_1:25014844-25014863	Msa0015480:CDS	30.0%
CTTAGGAATCTTTGATACTT+TGG	+	chr1_1:25015014-25015033	Msa0015480:CDS	30.0%
AAGCTATCTTGGAAAGTTTA+TGG	+	chr1_1:25015323-25015342	Msa0015480:intron	30.0%
GTCTTTGCTTGTTTACTTAT+TGG	+	chr1_1:25015474-25015493	Msa0015480:intron	30.0%
GCAATAAATCACAATGCATT+TGG	-	chr1_1:25015526-25015545	Msa0015470:intron	30.0%
ATCAATCATCCAAGAAACAT+TGG	+	chr1_1:25015855-25015874	Msa0015480:CDS	30.0%
GCTTATGGACATATTATAAG+TGG	+	chr1_1:25016118-25016137	Msa0015480:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_1	gene	25014606	25016862	25014606	ID=Msa0015480;Name=Msa0015480
chr1_1	mRNA	25014606	25016862	25014606	ID=Msa0015480-mRNA-1;Parent=Msa0015480;Name=Msa0015480-mRNA-1;_AED=0.30;_eAED=0.30;_QI=0\|0.5\|0.2\|1\|1\|1\|5\|0\|274
chr1_1	exon	25014606	25014638	25014606	ID=Msa0015480-mRNA-1:exon:8608;Parent=Msa0015480-mRNA-1
chr1_1	exon	25014811	25015095	25014811	ID=Msa0015480-mRNA-1:exon:8609;Parent=Msa0015480-mRNA-1
chr1_1	exon	25015638	25015896	25015638	ID=Msa0015480-mRNA-1:exon:8610;Parent=Msa0015480-mRNA-1
chr1_1	exon	25016440	25016549	25016440	ID=Msa0015480-mRNA-1:exon:8611;Parent=Msa0015480-mRNA-1
chr1_1	exon	25016725	25016862	25016725	ID=Msa0015480-mRNA-1:exon:8612;Parent=Msa0015480-mRNA-1
chr1_1	CDS	25014606	25014638	25014606	ID=Msa0015480-mRNA-1:cds;Parent=Msa0015480-mRNA-1
chr1_1	CDS	25014811	25015095	25014811	ID=Msa0015480-mRNA-1:cds;Parent=Msa0015480-mRNA-1
chr1_1	CDS	25015638	25015896	25015638	ID=Msa0015480-mRNA-1:cds;Parent=Msa0015480-mRNA-1
chr1_1	CDS	25016440	25016549	25016440	ID=Msa0015480-mRNA-1:cds;Parent=Msa0015480-mRNA-1
chr1_1	CDS	25016725	25016862	25016725	ID=Msa0015480-mRNA-1:cds;Parent=Msa0015480-mRNA-1

Gene Sequence

>Msa0015480

ATGGGAATGAGAGATTTTCACTTCAAAGTCAAGGCACTTCGGGTGAAACTCTCATGTTTAGGAAATAGAAAGGTAAGAAGAAATCATGGAGTTGGAAGCAAGCCATCATGGATGATGCCAATATCACATGGTCATCATGTGGTGGAGCATGATTGGATTAATGGTGATTCTGATGACTCTGAGTTTGATTCAGTTGTGATACAAAGAGAGCAAATTGGTGAGACAGAATTGTGGTACTTAGGAATCTTTGATACTTTGGTTGGAGATTGTGTTACCAAATATTTGCAAGCCAATTATTTTGACAAGAATCTCAAAGAGTCTCATTTGAGGAGAAAAGCCAAAGAAACACTCAAGAGAGCATACCTAGGTGCAAAAACAAAGATCAGAGAGACACAGAAATCAGATGAAACATGCATCATAGGTTCAACATCTGTGATGATAATAAATGGAGAAAAACTCGTGCTAGCCAACATCGGAGATTACAGAACTGTTTTGTGTAGAGATGGCGTAGCTTATCAGACAATTGGAAAGCACAATCAATCATCCAAGAAACATTGGTATCACAGACTTTTTTCAGGTAATGCAACAGGTACAAAGGCTTCTAAAGCTTCAGAACTTGTGGTTGGAAGTTATTGGATTGACTCTAGTACTGAATTTGTGATCTTGGCAAGCAATGGCATATGGGAGGTGATGAAGAACCAAGAAGCAGTGAATCTTATCAGGCACATAGAGGATCCACAAGAAGCAGCAGAGTGTTTGGCTAAGGAAGCATTGGTTAGGATGAGCAGAAGCCATATTTCATGTTTAATCATTCGGTTTGATTGA

Protein sequence

>Msa0015480

MGMRDFHFKVKALRVKLSCLGNRKVRRNHGVGSKPSWMMPISHGHHVVEHDWINGDSDDSEFDSVVIQREQIGETELWYLGIFDTLVGDCVTKYLQANYFDKNLKESHLRRKAKETLKRAYLGAKTKIRETQKSDETCIIGSTSVMIINGEKLVLANIGDYRTVLCRDGVAYQTIGKHNQSSKKHWYHRLFSGNATGTKASKASELVVGSYWIDSSTEFVILASNGIWEVMKNQEAVNLIRHIEDPQEAAECLAKEALVRMSRSHISCLIIRFD*