AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0262370

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0262370 MtrunA17_Chr2g0324921 97.917 336 7 0 1 336 1 336 0.0 681
Msa0262370 MtrunA17_Chr4g0040501 68.362 354 83 5 1 335 1 344 2.06e-173 485
Msa0262370 MtrunA17_Chr2g0282741 66.476 349 93 4 1 335 1 339 1.14e-162 457
Msa0262370 MtrunA17_Chr4g0006391 65.330 349 99 6 1 336 1 340 1.55e-157 444
Msa0262370 MtrunA17_Chr2g0282761 56.447 349 121 5 1 328 1 339 9.06e-132 379
Msa0262370 MtrunA17_Chr3g0138471 54.783 345 129 5 1 336 1 327 2.44e-125 362
Msa0262370 MtrunA17_Chr3g0093111 43.750 336 161 3 3 335 1 311 3.80e-94 282
Msa0262370 MtrunA17_Chr5g0415751 58.621 145 60 0 190 334 16 160 8.36e-57 182
Msa0262370 MtrunA17_Chr3g0124801 44.693 179 98 1 157 334 113 291 3.51e-50 169
Msa0262370 MtrunA17_Chr7g0269841 34.513 339 191 8 5 334 27 343 8.48e-49 167
Msa0262370 MtrunA17_Chr2g0277721 43.333 180 101 1 156 334 80 259 2.52e-47 160
Msa0262370 MtrunA17_Chr8g0334811 31.313 396 158 11 5 334 15 362 5.00e-34 129
Msa0262370 MtrunA17_Chr2g0322181 40.690 145 84 1 192 334 214 358 9.48e-29 115
Msa0262370 MtrunA17_Chr2g0322181 57.746 71 25 1 5 75 14 79 1.56e-11 65.1
Msa0262370 MtrunA17_Chr4g0025401 40.690 145 84 1 192 334 212 356 6.97e-28 112
Msa0262370 MtrunA17_Chr3g0091351 50.000 96 48 0 220 315 2 97 3.43e-26 100
Msa0262370 MtrunA17_Chr5g0426951 27.933 358 210 12 4 332 83 421 3.03e-23 100
Msa0262370 MtrunA17_Chr6g0478651 35.714 182 78 5 2 183 66 208 1.50e-21 95.5
Msa0262370 MtrunA17_Chr3g0087741 28.242 347 209 9 4 327 89 418 9.61e-21 92.4
Msa0262370 MtrunA17_Chr7g0263971 56.522 69 29 1 4 72 369 436 3.88e-20 90.9
Msa0262370 MtrunA17_Chr4g0010781 25.843 356 215 9 1 327 83 418 1.40e-19 89.0
Msa0262370 MtrunA17_Chr5g0415731 56.000 75 26 1 4 71 5 79 1.50e-19 84.0
Msa0262370 MtrunA17_Chr2g0333221 42.609 115 44 3 4 118 70 162 7.62e-18 84.3
Msa0262370 MtrunA17_Chr3g0123861 54.795 73 31 1 3 75 81 151 1.19e-17 83.6
Msa0262370 MtrunA17_Chr7g0271891 53.030 66 28 1 5 67 17 82 3.38e-17 77.8
Msa0262370 MtrunA17_Chr4g0021141 50.746 67 32 1 4 70 22 87 2.09e-15 72.0
Msa0262370 MtrunA17_Chr8g0344721 49.275 69 30 1 4 67 10 78 4.49e-15 73.6
Msa0262370 MtrunA17_Chr5g0399201 45.946 74 38 1 4 75 12 85 1.94e-14 73.9
Msa0262370 MtrunA17_Chr5g0427221 51.562 64 30 1 4 67 48 110 2.99e-14 69.3
Msa0262370 MtrunA17_Chr2g0295821 36.641 131 59 4 4 125 104 219 8.20e-14 71.6
Msa0262370 MtrunA17_Chr1g0159491 46.377 69 36 1 3 71 5 72 1.59e-13 65.1
Msa0262370 MtrunA17_Chr1g0209551 47.887 71 36 1 4 74 16 85 3.05e-13 70.1
Msa0262370 MtrunA17_Chr7g0242951 47.143 70 35 1 3 72 26 93 3.50e-13 70.5
Msa0262370 MtrunA17_Chr4g0044331 50.000 60 27 1 11 67 19 78 6.14e-13 65.9
Msa0262370 MtrunA17_Chr5g0414471 46.575 73 38 1 4 76 25 96 1.24e-12 68.2
Msa0262370 MtrunA17_Chr1g0173931 47.692 65 33 1 6 70 40 103 2.97e-12 66.2
Msa0262370 MtrunA17_Chr6g0460461 37.895 95 41 2 3 93 1 81 3.16e-12 65.1
Msa0262370 MtrunA17_Chr6g0460411 37.895 95 41 2 3 93 1 81 3.30e-12 65.1
Msa0262370 MtrunA17_Chr7g0258061 44.595 74 37 2 6 76 24 96 1.00e-11 64.7
Msa0262370 MtrunA17_Chr6g0460381 42.029 69 36 1 3 67 2 70 3.05e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0262370 AT2G20560.1 71.637 342 83 3 1 334 1 336 1.20e-165 465
Msa0262370 AT4G28480.1 69.801 351 85 6 1 334 1 347 7.92e-157 443
Msa0262370 AT3G08910.1 65.476 336 102 4 1 336 1 322 1.01e-154 437
Msa0262370 AT5G01390.1 59.710 345 118 5 1 335 1 334 3.18e-142 405
Msa0262370 AT5G25530.2 55.000 360 117 6 1 336 1 339 7.93e-135 387
Msa0262370 AT5G25530.1 53.804 368 117 7 1 336 1 347 6.34e-132 380
Msa0262370 AT1G10350.1 52.571 350 150 5 1 336 1 348 1.82e-124 361
Msa0262370 AT3G47940.1 54.000 350 145 4 1 336 1 348 1.64e-119 348
Msa0262370 AT1G59725.1 55.786 337 141 4 1 336 1 330 3.00e-119 347
Msa0262370 AT5G01390.2 53.550 338 92 5 1 335 1 276 3.25e-116 337
Msa0262370 AT4G28480.2 55.425 341 93 10 1 334 1 289 4.85e-99 294
Msa0262370 AT2G20550.1 58.242 273 85 4 84 334 18 283 6.03e-92 276
Msa0262370 AT2G20550.2 58.242 273 85 4 84 334 18 283 6.03e-92 276
Msa0262370 AT5G01390.3 45.562 338 80 6 1 335 1 237 3.72e-86 259
Msa0262370 AT5G01390.4 54.369 206 73 4 1 196 1 195 9.85e-64 202
Msa0262370 AT1G44160.1 46.629 178 95 0 157 334 176 353 9.27e-50 170
Msa0262370 AT1G11040.1 40.331 181 108 0 154 334 252 432 1.19e-45 161
Msa0262370 AT3G62600.1 34.393 346 192 9 2 335 24 346 3.11e-44 155
Msa0262370 AT5G22060.1 32.800 375 177 10 5 334 15 359 4.72e-39 143
Msa0262370 AT3G44110.1 33.428 353 203 8 5 334 15 358 1.73e-38 142
Msa0262370 AT3G44110.2 32.515 326 188 8 5 307 15 331 2.20e-30 118
Msa0262370 AT5G48030.1 26.816 358 209 13 4 327 94 432 4.50e-22 97.1
Msa0262370 AT1G80030.4 43.651 126 47 5 4 129 75 176 4.18e-19 88.6
Msa0262370 AT1G80030.2 43.651 126 47 5 4 129 75 176 5.30e-19 88.2
Msa0262370 AT1G80030.3 43.651 126 47 5 4 129 75 176 5.30e-19 88.2
Msa0262370 AT1G80030.1 43.651 126 47 5 4 129 75 176 5.30e-19 88.2
Msa0262370 AT2G22360.1 55.405 74 31 1 3 76 85 156 6.88e-18 84.7
Msa0262370 AT4G39960.1 41.228 114 44 3 4 117 85 175 1.51e-17 83.6
Msa0262370 AT4G39960.2 41.228 114 44 3 4 117 85 175 1.51e-17 83.6
Msa0262370 AT5G03160.1 53.623 69 31 1 4 72 370 437 2.74e-17 82.8
Msa0262370 AT1G56300.2 53.030 66 28 1 5 67 14 79 8.20e-17 75.9
Msa0262370 AT1G56300.1 53.030 66 28 1 5 67 14 79 9.66e-17 77.0
Msa0262370 AT2G35720.1 41.975 81 45 1 4 82 15 95 3.61e-14 73.6
Msa0262370 AT5G16650.2 50.746 67 32 1 4 70 11 76 6.02e-14 69.3
Msa0262370 AT5G16650.1 51.562 64 30 1 4 67 11 73 7.78e-14 68.2
Msa0262370 AT2G41000.2 46.377 69 33 1 3 67 2 70 3.95e-13 68.2
Msa0262370 AT3G17830.1 38.849 139 51 3 2 140 61 165 5.12e-13 70.1
Msa0262370 AT1G68370.1 47.887 71 36 1 4 74 17 86 8.74e-13 69.3
Msa0262370 AT2G41000.1 46.377 69 33 1 3 67 2 70 9.48e-13 66.2
Msa0262370 AT3G08970.1 48.000 75 35 2 3 77 26 96 1.04e-12 69.3
Msa0262370 AT3G08970.2 48.000 75 35 2 3 77 26 96 1.04e-12 69.3
Msa0262370 AT5G05750.1 34.266 143 73 4 4 146 114 235 1.07e-12 68.2
Msa0262370 AT5G06910.1 50.769 65 31 1 6 70 31 94 1.08e-12 67.8
Msa0262370 AT1G71000.2 46.970 66 32 1 5 67 9 74 3.24e-12 64.3
Msa0262370 AT2G21510.3 47.222 72 37 1 4 75 6 76 3.60e-12 67.0
Msa0262370 AT2G21510.1 47.222 72 37 1 4 75 6 76 3.60e-12 67.0
Msa0262370 AT1G59980.2 42.029 69 39 1 6 74 25 92 3.75e-12 67.0
Msa0262370 AT2G21510.2 47.222 72 37 1 4 75 6 76 4.01e-12 67.0
Msa0262370 AT2G21510.4 47.222 72 37 1 4 75 6 76 4.01e-12 67.0
Msa0262370 AT1G71000.1 46.970 66 32 1 5 67 9 74 5.18e-12 63.9
Msa0262370 AT2G21510.5 47.222 72 37 1 4 75 6 76 5.52e-12 66.2
Msa0262370 AT1G59980.1 42.029 69 39 1 6 74 25 92 6.19e-12 66.6
Msa0262370 AT3G14200.1 46.875 64 32 1 6 67 14 77 7.52e-12 64.7
Msa0262370 AT1G72416.3 45.455 66 34 1 4 67 5 70 1.43e-11 63.5
Msa0262370 AT5G49060.2 33.913 115 67 2 4 118 99 204 1.59e-11 65.1
Msa0262370 AT5G49060.1 33.913 115 67 2 4 118 99 204 1.59e-11 65.1
Msa0262370 AT1G72416.4 45.455 66 34 1 4 67 5 70 1.88e-11 62.8
Msa0262370 AT1G72416.1 45.455 66 34 1 4 67 5 70 2.05e-11 62.8
Msa0262370 AT5G37750.1 39.130 92 54 1 4 95 70 159 2.16e-11 63.5
Msa0262370 AT1G72416.2 45.455 66 34 1 4 67 5 70 2.63e-11 62.4
Msa0262370 AT2G33735.1 43.077 65 36 1 4 68 22 85 2.97e-11 60.5
Msa0262370 AT2G33735.2 43.077 65 36 1 4 68 22 85 2.97e-11 60.5
Msa0262370 AT1G24120.1 41.892 74 42 1 4 77 20 92 3.38e-11 64.3
Msa0262370 AT4G19570.1 51.515 66 30 1 3 68 65 128 4.05e-11 64.3

Find 124 sgRNAs with CRISPR-Local

Find 273 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCCTTCTTTGTTGGATCCTT+TGG 0.129787 2_2:-61240631 None:intergenic
GTTAATGTTACATTAAAATT+TGG 0.135986 2_2:+61240957 Msa0262370:three_prime_UTR
CAGGAGGCAATTGGGAAATT+TGG 0.163872 2_2:+61240818 Msa0262370:three_prime_UTR
TTCTGAGATTTGCTTAAATT+TGG 0.199073 2_2:-61237785 None:intergenic
CTCAAGTTTCCCTTCTTTGT+TGG 0.262625 2_2:-61240640 None:intergenic
ATATCAGCAGGTATGGTATT+TGG 0.272102 2_2:-61240400 None:intergenic
CCTTCTTTGTTGGATCCTTT+GGG 0.278902 2_2:-61240630 None:intergenic
ATTGGGAAATTTGGTTGTTA+TGG 0.283905 2_2:+61240827 Msa0262370:three_prime_UTR
CACAAATATGTGAATGCTTA+TGG 0.285402 2_2:-61241047 None:intergenic
AGAGCTGTTTATGATCAATA+TGG 0.297643 2_2:+61237970 Msa0262370:CDS
TCTCTTGAGGATATATATAA+AGG 0.310218 2_2:+61238285 Msa0262370:CDS
ACCAACATTATTCAACTTCA+TGG 0.313695 2_2:+61240993 Msa0262370:three_prime_UTR
TGGATGTTCATTTCCCTTCT+CGG 0.321746 2_2:-61240380 None:intergenic
TTTCTTCAAGTCATCGTCTT+TGG 0.326757 2_2:-61237695 None:intergenic
ATTGCCTCCTGTCAAGATTA+TGG 0.339096 2_2:-61240806 None:intergenic
GGATAAAGTAAGATATTAAT+TGG 0.350954 2_2:+61240736 Msa0262370:exon
TTGTTATTAGGGTTCTTATC+AGG 0.352145 2_2:-61237748 None:intergenic
GGGAGTGGCATTCCTTCTCT+TGG 0.354044 2_2:-61240610 None:intergenic
AGAGGTAGCAATGGCATGTT+TGG 0.355428 2_2:+61238174 Msa0262370:CDS
GGTGATCTTCATCTTCTTAG+TGG 0.355739 2_2:-61238310 None:intergenic
GGATGTTCATTTCCCTTCTC+GGG 0.357845 2_2:-61240379 None:intergenic
TTAATGTTACATTAAAATTT+GGG 0.367373 2_2:+61240958 Msa0262370:three_prime_UTR
TGACTACTACAAGCTTCTTC+AGG 0.376848 2_2:+61237660 Msa0262370:CDS
TAATCTTGACAGGAGGCAAT+TGG 0.388489 2_2:+61240809 Msa0262370:three_prime_UTR
CTACCAGAGGCATGAGAATC+AGG 0.390225 2_2:-61238027 None:intergenic
GCCGCAAAATCGCAGAAAAT+CGG 0.395524 2_2:+61237573 Msa0262370:five_prime_UTR
TTTGTCCCCTTCTTCCATCC+AGG 0.403890 2_2:-61240346 None:intergenic
GGAATGGGAGGGATGGGAAG+AGG 0.407566 2_2:+61238156 Msa0262370:CDS
CGAAATCCCTTTGGTGGAAT+GGG 0.411716 2_2:+61238141 Msa0262370:CDS
CTTATCAGGATGCCACTTCA+TGG 0.411951 2_2:-61237734 None:intergenic
CATAGCAACTTGTTCTCAAT+AGG 0.424637 2_2:-61238261 None:intergenic
CATTATTCAACTTCATGGTA+TGG 0.433834 2_2:+61240998 Msa0262370:three_prime_UTR
GAGCTGTTTATGATCAATAT+GGG 0.439767 2_2:+61237971 Msa0262370:CDS
GTGAATCTCACTACACTTGA+TGG 0.443477 2_2:+61240529 Msa0262370:CDS
TTTGGGGATCGAAATCCCTT+TGG 0.447674 2_2:+61238132 Msa0262370:CDS
TATGATCAATATGGGGAAGA+TGG 0.451224 2_2:+61237979 Msa0262370:CDS
GATTCTCATGCCTCTGGTAG+TGG 0.458632 2_2:+61238030 Msa0262370:CDS
TCGAAATCCCTTTGGTGGAA+TGG 0.459463 2_2:+61238140 Msa0262370:CDS
ATTATTCAACTTCATGGTAT+GGG 0.463867 2_2:+61240999 Msa0262370:three_prime_UTR
TTTGTTGGATCCTTTGGGAG+TGG 0.464104 2_2:-61240625 None:intergenic
AACTGATACGGAAAGTCATC+AGG 0.465543 2_2:-61238072 None:intergenic
GTTAGACAATGAGTTACATT+GGG 0.467007 2_2:+61240917 Msa0262370:three_prime_UTR
CTCTTGAGGATATATATAAA+GGG 0.467907 2_2:+61238286 Msa0262370:CDS
GATCCGAAGTGAGCCGAGTT+GGG 0.468185 2_2:-61240684 None:intergenic
TCTCTTGCAGAAGCCTTAGC+TGG 0.474179 2_2:+61240499 Msa0262370:CDS
CCCAAAGGATCCAACAAAGA+AGG 0.480916 2_2:+61240630 Msa0262370:CDS
GGGGATCGAAATCCCTTTGG+TGG 0.482474 2_2:+61238135 Msa0262370:CDS
AATCTTGACAGGAGGCAATT+GGG 0.488582 2_2:+61240810 Msa0262370:three_prime_UTR
TAGATTACCATAATCTTGAC+AGG 0.489863 2_2:+61240799 Msa0262370:three_prime_UTR
GTCAAACCTGGATGGAAGAA+GGG 0.491815 2_2:+61240340 Msa0262370:CDS
GAAAATGATATCAGCAGGTA+TGG 0.491993 2_2:-61240407 None:intergenic
TATGGTGATGCTGGAGGAAG+TGG 0.494603 2_2:+61238201 Msa0262370:CDS
TGTTGTTCATCCTGAGTATG+AGG 0.496005 2_2:+61240579 Msa0262370:CDS
TGATCCGAAGTGAGCCGAGT+TGG 0.497615 2_2:-61240685 None:intergenic
ACTGTGATCAAGAATTTCTC+TGG 0.501571 2_2:-61238331 None:intergenic
CTTTGGTGGAATGGGAGGGA+TGG 0.502219 2_2:+61238149 Msa0262370:CDS
TTTGGTGGAATGGGAGGGAT+GGG 0.503447 2_2:+61238150 Msa0262370:CDS
TGTCAAACCTGGATGGAAGA+AGG 0.504580 2_2:+61240339 Msa0262370:CDS
TTTGGATCCTATGGTGATGC+TGG 0.505971 2_2:+61238192 Msa0262370:CDS
GGTTAGACAATGAGTTACAT+TGG 0.506915 2_2:+61240916 Msa0262370:three_prime_UTR
ATCCCTCCCATTCCACCAAA+GGG 0.515203 2_2:-61238147 None:intergenic
ACAAGCTTCTTCAGGTTGAT+CGG 0.516804 2_2:+61237668 Msa0262370:CDS
GTCCGATGATTGAACTGATA+CGG 0.527889 2_2:-61238084 None:intergenic
ATGATCAATATGGGGAAGAT+GGG 0.529413 2_2:+61237980 Msa0262370:CDS
CCACCTGATTCTCATGCCTC+TGG 0.530969 2_2:+61238024 Msa0262370:CDS
AATGGCATGTTTGGATCCTA+TGG 0.531436 2_2:+61238183 Msa0262370:CDS
ACCATGAAGTTGAATAATGT+TGG 0.535476 2_2:-61240994 None:intergenic
AGAAGGGCAACAACCTGCCA+TGG 0.538305 2_2:+61237633 Msa0262370:exon
ATGATGAAAATGATATCAGC+AGG 0.541292 2_2:-61240412 None:intergenic
GCATGAGAATCAGGTGGTGG+TGG 0.542892 2_2:-61238018 None:intergenic
TCGGCTCACTTCGGATCAGA+AGG 0.545186 2_2:+61240690 Msa0262370:CDS
AAAGATCACTTTCCCCGAGA+AGG 0.545291 2_2:+61240366 Msa0262370:CDS
CATCCCTCCCATTCCACCAA+AGG 0.550801 2_2:-61238148 None:intergenic
ATATGGGGAAGATGGGCTGA+AGG 0.551422 2_2:+61237987 Msa0262370:CDS
CTCACTTCGGATCAGAAGGC+AGG 0.551807 2_2:+61240694 Msa0262370:CDS
GGAATGAAGAAAGTCTTGGC+TGG 0.552067 2_2:+61240715 Msa0262370:CDS
CTGTCGAAGAGAAAGAGAGA+AGG 0.555619 2_2:+61237616 Msa0262370:five_prime_UTR
TTCCGTATCAGTTCAATCAT+CGG 0.556341 2_2:+61238082 Msa0262370:CDS
GGAACAACCTCCTCATACTC+AGG 0.557628 2_2:-61240589 None:intergenic
GTGCAGCAAAGTCACATCGC+TGG 0.559149 2_2:+61240297 Msa0262370:intron
GCATCAAGGTCCTCCTCGTA+AGG 0.559593 2_2:+61238233 Msa0262370:CDS
ACAGAAAACTCGCCATGAAG+TGG 0.563345 2_2:+61237722 Msa0262370:CDS
AAGATCACTTTCCCCGAGAA+GGG 0.565073 2_2:+61240367 Msa0262370:CDS
CACAATGTGTTCACTCGTGA+AGG 0.567306 2_2:+61240448 Msa0262370:CDS
CCAAAGGATCCAACAAAGAA+GGG 0.568075 2_2:+61240631 Msa0262370:CDS
AGCAACTTGTTCTCAATAGG+AGG 0.568821 2_2:-61238258 None:intergenic
TGTCGAAGAGAAAGAGAGAA+GGG 0.570583 2_2:+61237617 Msa0262370:five_prime_UTR
TAGTAGTCAACACCCATGGC+AGG 0.572331 2_2:-61237646 None:intergenic
AATCCCTTTGGTGGAATGGG+AGG 0.575318 2_2:+61238144 Msa0262370:CDS
CAATTAGTGTCAAACCTGGA+TGG 0.581052 2_2:+61240332 Msa0262370:CDS
GGCAGGAATGAAGAAAGTCT+TGG 0.581140 2_2:+61240711 Msa0262370:CDS
ATTCCCAACTCGGCTCACTT+CGG 0.581525 2_2:+61240681 Msa0262370:CDS
GAGAAATTCTTGATCACAGT+GGG 0.581668 2_2:+61238334 Msa0262370:CDS
TCACGAGTGAACACATTGTG+TGG 0.583017 2_2:-61240445 None:intergenic
ACTTCCTCCAGCATCACCAT+AGG 0.584524 2_2:-61238199 None:intergenic
TATGGGGAAGATGGGCTGAA+GGG 0.585281 2_2:+61237988 Msa0262370:CDS
TTGACAATTAGTGTCAAACC+TGG 0.594845 2_2:+61240328 Msa0262370:CDS
AGTGGAGTCAATGTGCATCA+AGG 0.595190 2_2:+61238219 Msa0262370:CDS
TCTCATGCCTCTGGTAGTGG+TGG 0.595916 2_2:+61238033 Msa0262370:CDS
ATCCCTTTGGTGGAATGGGA+GGG 0.596841 2_2:+61238145 Msa0262370:CDS
TTATTCAACTTCATGGTATG+GGG 0.599396 2_2:+61241000 Msa0262370:three_prime_UTR
GAAGGGCAACAACCTGCCAT+GGG 0.605250 2_2:+61237634 Msa0262370:exon
ATTCACTGTACAACCAGCTA+AGG 0.605987 2_2:-61240512 None:intergenic
GCTTAGGTACAATACAATCG+TGG 0.609230 2_2:+61237493 None:intergenic
GAGGAGGTTGTTCCAAGAGA+AGG 0.609654 2_2:+61240598 Msa0262370:CDS
GAGGCATGAGAATCAGGTGG+TGG 0.609693 2_2:-61238021 None:intergenic
AGAAGGAATGCCACTCCCAA+AGG 0.611731 2_2:+61240615 Msa0262370:CDS
GATGTTCATTTCCCTTCTCG+GGG 0.615186 2_2:-61240378 None:intergenic
CTTAGGTACAATACAATCGT+GGG 0.615629 2_2:+61237494 None:intergenic
TCAAACCTGGATGGAAGAAG+GGG 0.618407 2_2:+61240341 Msa0262370:CDS
TCAATAGGAGGCGCCTTACG+AGG 0.622058 2_2:-61238246 None:intergenic
TCAACGTCAAATTCCCAACT+CGG 0.622716 2_2:+61240671 Msa0262370:CDS
CAAGTTGCTATGTTCTCTTG+AGG 0.636918 2_2:+61238272 Msa0262370:CDS
GGGATGGGAAGAGGTAGCAA+TGG 0.641843 2_2:+61238165 Msa0262370:CDS
CAACAAAGAAGGGAAACTTG+AGG 0.650514 2_2:+61240641 Msa0262370:CDS
GGATCCTATGGTGATGCTGG+AGG 0.665018 2_2:+61238195 Msa0262370:CDS
ATTACCATAATCTTGACAGG+AGG 0.665326 2_2:+61240802 Msa0262370:three_prime_UTR
CTTGTAGTAGTCAACACCCA+TGG 0.668721 2_2:-61237650 None:intergenic
ATAGGAGGCGCCTTACGAGG+AGG 0.673195 2_2:-61238243 None:intergenic
AGCTGTTTATGATCAATATG+GGG 0.674859 2_2:+61237972 Msa0262370:CDS
TCTACTACCACCACTACCAG+AGG 0.706463 2_2:-61238040 None:intergenic
CCAGAGGCATGAGAATCAGG+TGG 0.707871 2_2:-61238024 None:intergenic
TGTTCATCCTGAGTATGAGG+AGG 0.716066 2_2:+61240582 Msa0262370:CDS
AGAGAAATTCTTGATCACAG+TGG 0.741173 2_2:+61238333 Msa0262370:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAGAACTTTTTTTTTTTTTT+TGG + chr2_2:61239222-61239241 Msa0262370:intron 10.0%
!!! AGAACTTTTTTTTTTTTTTT+GGG + chr2_2:61239223-61239242 Msa0262370:intron 10.0%
!!! ATTTAAAATTTTGAAAAAGT+GGG - chr2_2:61239385-61239404 None:intergenic 10.0%
!!! AATTTAAAATTTTGAAAAAG+TGG - chr2_2:61239386-61239405 None:intergenic 10.0%
!! TTAATGTTACATTAAAATTT+GGG + chr2_2:61240958-61240977 Msa0262370:three_prime_UTR 10.0%
!!! ATGATGTAAGTTTTTTTTAA+AGG + chr2_2:61237812-61237831 Msa0262370:intron 15.0%
!!! TGATGTAAGTTTTTTTTAAA+GGG + chr2_2:61237813-61237832 Msa0262370:intron 15.0%
!!! CTTAAATTTTCAATTTTTAG+TGG + chr2_2:61237839-61237858 Msa0262370:intron 15.0%
!! TATAAATATCTAATAACTGA+TGG - chr2_2:61238411-61238430 None:intergenic 15.0%
!! TAAACAATTACTATAATTCT+AGG - chr2_2:61239069-61239088 None:intergenic 15.0%
!! TATAAATCAACATTATGATA+TGG - chr2_2:61239635-61239654 None:intergenic 15.0%
!!! TAGTATAGTAATCTATTTTT+TGG + chr2_2:61240866-61240885 Msa0262370:three_prime_UTR 15.0%
!!! TATTTTTTGGAATGATATTT+TGG + chr2_2:61240879-61240898 Msa0262370:three_prime_UTR 15.0%
!! GTTAATGTTACATTAAAATT+TGG + chr2_2:61240957-61240976 Msa0262370:three_prime_UTR 15.0%
!!! GGAATTTGTTTTTTGTTATT+AGG + chr2_2:61237927-61237946 Msa0262370:intron 20.0%
!!! ATATTTTTGCTGAGTTTTTT+GGG + chr2_2:61238115-61238134 Msa0262370:CDS 20.0%
!! TCATAATATTAGATAGATAG+TGG + chr2_2:61238619-61238638 Msa0262370:intron 20.0%
!! CATAATATTAGATAGATAGT+GGG + chr2_2:61238620-61238639 Msa0262370:intron 20.0%
!! ATAATATTAGATAGATAGTG+GGG + chr2_2:61238621-61238640 Msa0262370:intron 20.0%
!! GTTCTATTAGAAAAAATTCA+AGG - chr2_2:61239208-61239227 None:intergenic 20.0%
!!! CTTTTTTTTTTTTTTTGGGT+AGG + chr2_2:61239227-61239246 Msa0262370:intron 20.0%
!! TTGATTTATAGTAGTATGTT+TGG + chr2_2:61239645-61239664 Msa0262370:intron 20.0%
!! TGATTTATAGTAGTATGTTT+GGG + chr2_2:61239646-61239665 Msa0262370:intron 20.0%
!! TTTAATGTTGCAAATTACAA+TGG + chr2_2:61239890-61239909 Msa0262370:intron 20.0%
!!! TTGATGTTTTTCTAGTATAA+TGG + chr2_2:61240115-61240134 Msa0262370:intron 20.0%
!! ATTCTAGATGTTTAAATTAG+AGG - chr2_2:61240168-61240187 None:intergenic 20.0%
!! AAAAAACAAAGTCATCAATA+GGG - chr2_2:61240263-61240282 None:intergenic 20.0%
!! AAAAAAACAAAGTCATCAAT+AGG - chr2_2:61240264-61240283 None:intergenic 20.0%
!! GGATAAAGTAAGATATTAAT+TGG + chr2_2:61240736-61240755 Msa0262370:exon 20.0%
!!! TGATATTTTGGTCTATTTTA+TGG + chr2_2:61240891-61240910 Msa0262370:three_prime_UTR 20.0%
!!! GTTCAAGAAATTTTAACGTT+GGG - chr2_2:61237534-61237553 None:intergenic 25.0%
!!! AGTTCAAGAAATTTTAACGT+TGG - chr2_2:61237535-61237554 None:intergenic 25.0%
!!! CAGCTTCTTTTTTGTTATTA+GGG - chr2_2:61237762-61237781 None:intergenic 25.0%
!!! TCAGCTTCTTTTTTGTTATT+AGG - chr2_2:61237763-61237782 None:intergenic 25.0%
!!! TTCTGAGATTTGCTTAAATT+TGG - chr2_2:61237788-61237807 None:intergenic 25.0%
!!! AAAGTTTTGTTTTTTGCATC+TGG + chr2_2:61237884-61237903 Msa0262370:intron 25.0%
!!! AAGTTTTGTTTTTTGCATCT+GGG + chr2_2:61237885-61237904 Msa0262370:intron 25.0%
!!! GATATTTTTGCTGAGTTTTT+TGG + chr2_2:61238114-61238133 Msa0262370:CDS 25.0%
!!! TATTTTTGCTGAGTTTTTTG+GGG + chr2_2:61238116-61238135 Msa0262370:CDS 25.0%
! TCTCTTGAGGATATATATAA+AGG + chr2_2:61238285-61238304 Msa0262370:CDS 25.0%
! CTCTTGAGGATATATATAAA+GGG + chr2_2:61238286-61238305 Msa0262370:CDS 25.0%
!! TAATATTAGATAGATAGTGG+GGG + chr2_2:61238622-61238641 Msa0262370:intron 25.0%
! TAATGAGTTGTATCGAAATA+TGG + chr2_2:61238828-61238847 Msa0262370:intron 25.0%
! TTGTATCGAAATATGGTTTA+TGG + chr2_2:61238835-61238854 Msa0262370:intron 25.0%
! GAACCATCAATACTTAAAAT+TGG - chr2_2:61239143-61239162 None:intergenic 25.0%
! CGAGTTATATTGGTATAAAT+TGG + chr2_2:61239495-61239514 Msa0262370:intron 25.0%
! GATTTATAGTAGTATGTTTG+GGG + chr2_2:61239647-61239666 Msa0262370:intron 25.0%
! ATTTATAGTAGTATGTTTGG+GGG + chr2_2:61239648-61239667 Msa0262370:intron 25.0%
!!! TCTGGTTTTTGAGATTATAT+TGG + chr2_2:61239775-61239794 Msa0262370:intron 25.0%
! TTGTAACATTAAACAAGAGA+AGG - chr2_2:61239980-61239999 None:intergenic 25.0%
!! TCTCTTGTTTAATGTTACAA+CGG + chr2_2:61239980-61239999 Msa0262370:intron 25.0%
!! TTTAAACTGTTGTTATTGCT+AGG + chr2_2:61240088-61240107 Msa0262370:intron 25.0%
!! TTAAACTGTTGTTATTGCTA+GGG + chr2_2:61240089-61240108 Msa0262370:intron 25.0%
! GATATTAATACAGACTAACA+TGG - chr2_2:61240146-61240165 None:intergenic 25.0%
!! ATTAATTGGTACATCTTTTG+AGG + chr2_2:61240750-61240769 Msa0262370:three_prime_UTR 25.0%
!!! ATTTTGGTCTATTTTATGGT+TGG + chr2_2:61240895-61240914 Msa0262370:three_prime_UTR 25.0%
! ATTATTCAACTTCATGGTAT+GGG + chr2_2:61240999-61241018 Msa0262370:three_prime_UTR 25.0%
! TTGTTATTAGGGTTCTTATC+AGG - chr2_2:61237751-61237770 None:intergenic 30.0%
AGAGCTGTTTATGATCAATA+TGG + chr2_2:61237970-61237989 Msa0262370:CDS 30.0%
GAGCTGTTTATGATCAATAT+GGG + chr2_2:61237971-61237990 Msa0262370:CDS 30.0%
AGCTGTTTATGATCAATATG+GGG + chr2_2:61237972-61237991 Msa0262370:CDS 30.0%
AACTCAGCAAAAATATCATC+AGG - chr2_2:61238111-61238130 None:intergenic 30.0%
! ACAGCATTGAAAAAAGAACT+AGG - chr2_2:61238380-61238399 None:intergenic 30.0%
ATTGCTGAGATTTACATGTT+TGG + chr2_2:61238470-61238489 Msa0262370:intron 30.0%
! TGTAGCATTTCTAGATTTAC+AGG + chr2_2:61238507-61238526 Msa0262370:intron 30.0%
TAGAAAAGATTGTGGGAAAT+AGG + chr2_2:61238657-61238676 Msa0262370:intron 30.0%
AAGTATTATCTCACTTAGTG+GGG - chr2_2:61238884-61238903 None:intergenic 30.0%
CAAGTATTATCTCACTTAGT+GGG - chr2_2:61238885-61238904 None:intergenic 30.0%
ACAAGTATTATCTCACTTAG+TGG - chr2_2:61238886-61238905 None:intergenic 30.0%
TGGATGAAATAGCAAAAACA+TGG - chr2_2:61238945-61238964 None:intergenic 30.0%
ATCTGCTTCTTTATTGAAAG+AGG + chr2_2:61239036-61239055 Msa0262370:intron 30.0%
ATACTTAAAATTGGAGGAAG+AGG - chr2_2:61239134-61239153 None:intergenic 30.0%
CCATCAATACTTAAAATTGG+AGG - chr2_2:61239140-61239159 None:intergenic 30.0%
!!! CCTCCAATTTTAAGTATTGA+TGG + chr2_2:61239137-61239156 Msa0262370:intron 30.0%
AAACATTCAATCTAACGTGT+CGG - chr2_2:61239300-61239319 None:intergenic 30.0%
CAATATAACTCGCTTCATAT+CGG - chr2_2:61239487-61239506 None:intergenic 30.0%
ATAGACAATAAGTAGCTTCT+TGG + chr2_2:61239575-61239594 Msa0262370:intron 30.0%
AATACTAGATTTGTGATGCT+TGG + chr2_2:61239677-61239696 Msa0262370:intron 30.0%
TGTCATAAAGTTTATGTCTC+TGG + chr2_2:61239757-61239776 Msa0262370:intron 30.0%
TTGCAACATTAAACAAGAGA+AGG - chr2_2:61239883-61239902 None:intergenic 30.0%
TTAAACAAGAGAAGGACAAT+GGG - chr2_2:61239972-61239991 None:intergenic 30.0%
ATTAAACAAGAGAAGGACAA+TGG - chr2_2:61239973-61239992 None:intergenic 30.0%
! AACATCTAGAATTCGAGATA+AGG + chr2_2:61240176-61240195 Msa0262370:intron 30.0%
! ACATCTAGAATTCGAGATAA+GGG + chr2_2:61240177-61240196 Msa0262370:intron 30.0%
ATGCATGATATCTCTCATTA+TGG + chr2_2:61240216-61240235 Msa0262370:intron 30.0%
ATGATGAAAATGATATCAGC+AGG - chr2_2:61240415-61240434 None:intergenic 30.0%
TAGATTACCATAATCTTGAC+AGG + chr2_2:61240799-61240818 Msa0262370:three_prime_UTR 30.0%
ATTGGGAAATTTGGTTGTTA+TGG + chr2_2:61240827-61240846 Msa0262370:three_prime_UTR 30.0%
GTTAGACAATGAGTTACATT+GGG + chr2_2:61240917-61240936 Msa0262370:three_prime_UTR 30.0%
ACCAACATTATTCAACTTCA+TGG + chr2_2:61240993-61241012 Msa0262370:three_prime_UTR 30.0%
ACCATGAAGTTGAATAATGT+TGG - chr2_2:61240997-61241016 None:intergenic 30.0%
CATTATTCAACTTCATGGTA+TGG + chr2_2:61240998-61241017 Msa0262370:three_prime_UTR 30.0%
TTATTCAACTTCATGGTATG+GGG + chr2_2:61241000-61241019 Msa0262370:three_prime_UTR 30.0%
CACAAATATGTGAATGCTTA+TGG - chr2_2:61241050-61241069 None:intergenic 30.0%
! CTTTCTCTTCGACAGTTTTA+CGG - chr2_2:61237611-61237630 None:intergenic 35.0%
TTTCTTCAAGTCATCGTCTT+TGG - chr2_2:61237698-61237717 None:intergenic 35.0%
TATGATCAATATGGGGAAGA+TGG + chr2_2:61237979-61237998 Msa0262370:CDS 35.0%
ATGATCAATATGGGGAAGAT+GGG + chr2_2:61237980-61237999 Msa0262370:CDS 35.0%
TTCCGTATCAGTTCAATCAT+CGG + chr2_2:61238082-61238101 Msa0262370:CDS 35.0%
! CATAGCAACTTGTTCTCAAT+AGG - chr2_2:61238264-61238283 None:intergenic 35.0%
ACTGTGATCAAGAATTTCTC+TGG - chr2_2:61238334-61238353 None:intergenic 35.0%
AGAGAAATTCTTGATCACAG+TGG + chr2_2:61238333-61238352 Msa0262370:CDS 35.0%
GAGAAATTCTTGATCACAGT+GGG + chr2_2:61238334-61238353 Msa0262370:CDS 35.0%
CATTGAAAAAAGAACTAGGC+AGG - chr2_2:61238376-61238395 None:intergenic 35.0%
AAAGTAGCTCAGTTTCAGTT+TGG + chr2_2:61238434-61238453 Msa0262370:intron 35.0%
TAATTGGAGAAGAGACTGAT+TGG + chr2_2:61238559-61238578 Msa0262370:intron 35.0%
ACCCATAGTAGAAAAGATTG+TGG + chr2_2:61238649-61238668 Msa0262370:intron 35.0%
! CCCACAATCTTTTCTACTAT+GGG - chr2_2:61238653-61238672 None:intergenic 35.0%
CCCATAGTAGAAAAGATTGT+GGG + chr2_2:61238650-61238669 Msa0262370:intron 35.0%
! TCCCACAATCTTTTCTACTA+TGG - chr2_2:61238654-61238673 None:intergenic 35.0%
ACCTGTACTGTAGATTATGT+AGG + chr2_2:61238709-61238728 Msa0262370:intron 35.0%
TCCTACATAATCTACAGTAC+AGG - chr2_2:61238713-61238732 None:intergenic 35.0%
GTACTGTAGATTATGTAGGA+AGG + chr2_2:61238713-61238732 Msa0262370:intron 35.0%
TTAAAATTGGAGGAAGAGGA+AGG - chr2_2:61239130-61239149 None:intergenic 35.0%
GTGTGACATGTATCACTATA+TGG + chr2_2:61239329-61239348 Msa0262370:intron 35.0%
TGTCTATGTCAATGCTTCAT+AGG + chr2_2:61239452-61239471 Msa0262370:intron 35.0%
CAATGCTTCATAGGTTAACA+TGG + chr2_2:61239461-61239480 Msa0262370:intron 35.0%
CGATATGAAGCGAGTTATAT+TGG + chr2_2:61239485-61239504 Msa0262370:intron 35.0%
TCCTATTTCATTACAGTCCA+GGG + chr2_2:61239798-61239817 Msa0262370:intron 35.0%
TGTTGCAAATTACAATGGCT+TGG + chr2_2:61239895-61239914 Msa0262370:intron 35.0%
!! ATGGCTTGGTGTTTGTTAAA+CGG + chr2_2:61239909-61239928 Msa0262370:intron 35.0%
TGTTAAACGGGATCAATAAC+TGG + chr2_2:61239922-61239941 Msa0262370:intron 35.0%
TAAACAAGAGAAGGACAATG+GGG - chr2_2:61239971-61239990 None:intergenic 35.0%
TGTTTAATGTTACAACGGCT+TGG + chr2_2:61239985-61240004 Msa0262370:intron 35.0%
TGTTAAACGGGATCAATAAC+TGG + chr2_2:61239922-61239941 Msa0262370:intron 35.0%
TCCCAAACAATATGCACAAT+GGG - chr2_2:61240062-61240081 None:intergenic 35.0%
ATCCCAAACAATATGCACAA+TGG - chr2_2:61240063-61240082 None:intergenic 35.0%
ATTGTGCATATTGTTTGGGA+TGG + chr2_2:61240062-61240081 Msa0262370:intron 35.0%
TTGACAATTAGTGTCAAACC+TGG + chr2_2:61240328-61240347 Msa0262370:CDS 35.0%
ATATCAGCAGGTATGGTATT+TGG - chr2_2:61240403-61240422 None:intergenic 35.0%
GAAAATGATATCAGCAGGTA+TGG - chr2_2:61240410-61240429 None:intergenic 35.0%
ATTACCATAATCTTGACAGG+AGG + chr2_2:61240802-61240821 Msa0262370:three_prime_UTR 35.0%
GGTTAGACAATGAGTTACAT+TGG + chr2_2:61240916-61240935 Msa0262370:three_prime_UTR 35.0%
! TTTTGCGGCGAATTTGTGAA+GGG - chr2_2:61237562-61237581 None:intergenic 40.0%
! ATTTTGCGGCGAATTTGTGA+AGG - chr2_2:61237563-61237582 None:intergenic 40.0%
! TCCGATTTTCTGCGATTTTG+CGG - chr2_2:61237577-61237596 None:intergenic 40.0%
TGTCGAAGAGAAAGAGAGAA+GGG + chr2_2:61237617-61237636 Msa0262370:five_prime_UTR 40.0%
TGACTACTACAAGCTTCTTC+AGG + chr2_2:61237660-61237679 Msa0262370:CDS 40.0%
!! ACAAGCTTCTTCAGGTTGAT+CGG + chr2_2:61237668-61237687 Msa0262370:CDS 40.0%
AACTGATACGGAAAGTCATC+AGG - chr2_2:61238075-61238094 None:intergenic 40.0%
GTCCGATGATTGAACTGATA+CGG - chr2_2:61238087-61238106 None:intergenic 40.0%
AATGGCATGTTTGGATCCTA+TGG + chr2_2:61238183-61238202 Msa0262370:CDS 40.0%
! AGCAACTTGTTCTCAATAGG+AGG - chr2_2:61238261-61238280 None:intergenic 40.0%
CAAGTTGCTATGTTCTCTTG+AGG + chr2_2:61238272-61238291 Msa0262370:CDS 40.0%
GGTGATCTTCATCTTCTTAG+TGG - chr2_2:61238313-61238332 None:intergenic 40.0%
! ACAAACCCTCGTGGATTAAT+TGG + chr2_2:61238543-61238562 Msa0262370:intron 40.0%
CTTCTCCAATTAATCCACGA+GGG - chr2_2:61238551-61238570 None:intergenic 40.0%
TCTTCTCCAATTAATCCACG+AGG - chr2_2:61238552-61238571 None:intergenic 40.0%
!! CTGATTGGTCTCTTATAGCT+CGG + chr2_2:61238574-61238593 Msa0262370:intron 40.0%
AGATTGTGGGAAATAGGCTT+AGG + chr2_2:61238663-61238682 Msa0262370:intron 40.0%
GAAATAGGCTTAGGTGGTTT+AGG + chr2_2:61238672-61238691 Msa0262370:intron 40.0%
GAGGATAGTCAAATCACTAG+AGG + chr2_2:61238751-61238770 Msa0262370:intron 40.0%
ATTATCTCACTTAGTGGGGT+TGG - chr2_2:61238880-61238899 None:intergenic 40.0%
CCAGCATATTCTCTACAACA+TGG - chr2_2:61238965-61238984 None:intergenic 40.0%
CCATGTTGTAGAGAATATGC+TGG + chr2_2:61238962-61238981 Msa0262370:intron 40.0%
GTTGTAGAGAATATGCTGGT+AGG + chr2_2:61238966-61238985 Msa0262370:intron 40.0%
!! GTAGGTTTTGTAGTTGTGGT+TGG + chr2_2:61238984-61239003 Msa0262370:intron 40.0%
! CTGAGTTTTACACTCAAGTC+AGG + chr2_2:61239178-61239197 Msa0262370:intron 40.0%
!! TGAAGCATTGACATAGACAC+CGG - chr2_2:61239451-61239470 None:intergenic 40.0%
GTCCTATTTCATTACAGTCC+AGG + chr2_2:61239797-61239816 Msa0262370:intron 40.0%
TCCCTGGACTGTAATGAAAT+AGG - chr2_2:61239802-61239821 None:intergenic 40.0%
!! TGGCTTGGTGTTTGTTAAAC+GGG + chr2_2:61239910-61239929 Msa0262370:intron 40.0%
! GGGGTTAGTACATTAGACTA+AGG - chr2_2:61239952-61239971 None:intergenic 40.0%
!! ACGGCTTGGTGTTTGTTAAA+CGG + chr2_2:61239999-61240018 Msa0262370:intron 40.0%
! GGGGTTAGTACATTAGACTA+AGG - chr2_2:61239952-61239971 None:intergenic 40.0%
ACCCCATTGTGCATATTGTT+TGG + chr2_2:61240057-61240076 Msa0262370:intron 40.0%
CCCAAACAATATGCACAATG+GGG - chr2_2:61240061-61240080 None:intergenic 40.0%
CCCCATTGTGCATATTGTTT+GGG + chr2_2:61240058-61240077 Msa0262370:intron 40.0%
CAATTAGTGTCAAACCTGGA+TGG + chr2_2:61240332-61240351 Msa0262370:CDS 40.0%
TGGATGTTCATTTCCCTTCT+CGG - chr2_2:61240383-61240402 None:intergenic 40.0%
ATTCACTGTACAACCAGCTA+AGG - chr2_2:61240515-61240534 None:intergenic 40.0%
GTGAATCTCACTACACTTGA+TGG + chr2_2:61240529-61240548 Msa0262370:CDS 40.0%
TGTTGTTCATCCTGAGTATG+AGG + chr2_2:61240579-61240598 Msa0262370:CDS 40.0%
CCTTCTTTGTTGGATCCTTT+GGG - chr2_2:61240633-61240652 None:intergenic 40.0%
CCAAAGGATCCAACAAAGAA+GGG + chr2_2:61240631-61240650 Msa0262370:CDS 40.0%
CTCAAGTTTCCCTTCTTTGT+TGG - chr2_2:61240643-61240662 None:intergenic 40.0%
CAACAAAGAAGGGAAACTTG+AGG + chr2_2:61240641-61240660 Msa0262370:CDS 40.0%
TCAACGTCAAATTCCCAACT+CGG + chr2_2:61240671-61240690 Msa0262370:CDS 40.0%
ATTGCCTCCTGTCAAGATTA+TGG - chr2_2:61240809-61240828 None:intergenic 40.0%
TAATCTTGACAGGAGGCAAT+TGG + chr2_2:61240809-61240828 Msa0262370:three_prime_UTR 40.0%
AATCTTGACAGGAGGCAATT+GGG + chr2_2:61240810-61240829 Msa0262370:three_prime_UTR 40.0%
GCCGCAAAATCGCAGAAAAT+CGG + chr2_2:61237573-61237592 Msa0262370:five_prime_UTR 45.0%
! GACAGTTTTACGGCACTTTC+TGG - chr2_2:61237601-61237620 None:intergenic 45.0%
CTGTCGAAGAGAAAGAGAGA+AGG + chr2_2:61237616-61237635 Msa0262370:five_prime_UTR 45.0%
CTTGTAGTAGTCAACACCCA+TGG - chr2_2:61237653-61237672 None:intergenic 45.0%
ACAGAAAACTCGCCATGAAG+TGG + chr2_2:61237722-61237741 Msa0262370:CDS 45.0%
! CTTATCAGGATGCCACTTCA+TGG - chr2_2:61237737-61237756 None:intergenic 45.0%
!!! TCTGGGTCATGCTGATTTTG+TGG + chr2_2:61237902-61237921 Msa0262370:intron 45.0%
!!! GGTCATGCTGATTTTGTGGT+GGG + chr2_2:61237906-61237925 Msa0262370:intron 45.0%
TTTGGGGATCGAAATCCCTT+TGG + chr2_2:61238132-61238151 Msa0262370:CDS 45.0%
TCGAAATCCCTTTGGTGGAA+TGG + chr2_2:61238140-61238159 Msa0262370:CDS 45.0%
CGAAATCCCTTTGGTGGAAT+GGG + chr2_2:61238141-61238160 Msa0262370:CDS 45.0%
AGAGGTAGCAATGGCATGTT+TGG + chr2_2:61238174-61238193 Msa0262370:CDS 45.0%
! TTTGGATCCTATGGTGATGC+TGG + chr2_2:61238192-61238211 Msa0262370:CDS 45.0%
AGTGGAGTCAATGTGCATCA+AGG + chr2_2:61238219-61238238 Msa0262370:CDS 45.0%
AACCAAAGTACAAACCCTCG+TGG + chr2_2:61238534-61238553 Msa0262370:intron 45.0%
ATCCACGAGGGTTTGTACTT+TGG - chr2_2:61238539-61238558 None:intergenic 45.0%
TTGTGGGAAATAGGCTTAGG+TGG + chr2_2:61238666-61238685 Msa0262370:intron 45.0%
AGGAAGGACAGTAGATGACA+TGG + chr2_2:61238729-61238748 Msa0262370:intron 45.0%
AAGGACAGTAGATGACATGG+AGG + chr2_2:61238732-61238751 Msa0262370:intron 45.0%
AGTCAAATCACTAGAGGCAG+AGG + chr2_2:61238757-61238776 Msa0262370:intron 45.0%
!! GCTGGTAGGTTTTGTAGTTG+TGG + chr2_2:61238980-61238999 Msa0262370:intron 45.0%
! TGTGGTTGGTGCAATGTATG+TGG + chr2_2:61238998-61239017 Msa0262370:intron 45.0%
!! TGTATGTGGTGCCATTTGTG+TGG + chr2_2:61239012-61239031 Msa0262370:intron 45.0%
AGAAGCAGATGCCACACAAA+TGG - chr2_2:61239026-61239045 None:intergenic 45.0%
!! CTATATGGCATCGACACATG+TGG + chr2_2:61239344-61239363 Msa0262370:intron 45.0%
TGACACAGACACTTTGACAC+TGG - chr2_2:61239413-61239432 None:intergenic 45.0%
! CAAAGTGTCTGTGTCATGTC+TGG + chr2_2:61239417-61239436 Msa0262370:intron 45.0%
AGGGATGTAGAAGTCTTCTG+CGG + chr2_2:61239817-61239836 Msa0262370:intron 45.0%
!! CGGCTTGGTGTTTGTTAAAC+GGG + chr2_2:61240000-61240019 Msa0262370:intron 45.0%
TGTCAAACCTGGATGGAAGA+AGG + chr2_2:61240339-61240358 Msa0262370:CDS 45.0%
GTCAAACCTGGATGGAAGAA+GGG + chr2_2:61240340-61240359 Msa0262370:CDS 45.0%
TCAAACCTGGATGGAAGAAG+GGG + chr2_2:61240341-61240360 Msa0262370:CDS 45.0%
AAAGATCACTTTCCCCGAGA+AGG + chr2_2:61240366-61240385 Msa0262370:CDS 45.0%
AAGATCACTTTCCCCGAGAA+GGG + chr2_2:61240367-61240386 Msa0262370:CDS 45.0%
GATGTTCATTTCCCTTCTCG+GGG - chr2_2:61240381-61240400 None:intergenic 45.0%
GGATGTTCATTTCCCTTCTC+GGG - chr2_2:61240382-61240401 None:intergenic 45.0%
TCACGAGTGAACACATTGTG+TGG - chr2_2:61240448-61240467 None:intergenic 45.0%
CACAATGTGTTCACTCGTGA+AGG + chr2_2:61240448-61240467 Msa0262370:CDS 45.0%
TGTTCATCCTGAGTATGAGG+AGG + chr2_2:61240582-61240601 Msa0262370:CDS 45.0%
! TTTGTTGGATCCTTTGGGAG+TGG - chr2_2:61240628-61240647 None:intergenic 45.0%
CCCAAAGGATCCAACAAAGA+AGG + chr2_2:61240630-61240649 Msa0262370:CDS 45.0%
CCCTTCTTTGTTGGATCCTT+TGG - chr2_2:61240634-61240653 None:intergenic 45.0%
GGCAGGAATGAAGAAAGTCT+TGG + chr2_2:61240711-61240730 Msa0262370:CDS 45.0%
GGAATGAAGAAAGTCTTGGC+TGG + chr2_2:61240715-61240734 Msa0262370:CDS 45.0%
CAGGAGGCAATTGGGAAATT+TGG + chr2_2:61240818-61240837 Msa0262370:three_prime_UTR 45.0%
TAGTAGTCAACACCCATGGC+AGG - chr2_2:61237649-61237668 None:intergenic 50.0%
!!! GGGTCATGCTGATTTTGTGG+TGG + chr2_2:61237905-61237924 Msa0262370:intron 50.0%
!! ATATGGGGAAGATGGGCTGA+AGG + chr2_2:61237987-61238006 Msa0262370:CDS 50.0%
!! TATGGGGAAGATGGGCTGAA+GGG + chr2_2:61237988-61238007 Msa0262370:CDS 50.0%
! CTACCAGAGGCATGAGAATC+AGG - chr2_2:61238030-61238049 None:intergenic 50.0%
GATTCTCATGCCTCTGGTAG+TGG + chr2_2:61238030-61238049 Msa0262370:CDS 50.0%
TCTACTACCACCACTACCAG+AGG - chr2_2:61238043-61238062 None:intergenic 50.0%
AATCCCTTTGGTGGAATGGG+AGG + chr2_2:61238144-61238163 Msa0262370:CDS 50.0%
ATCCCTTTGGTGGAATGGGA+GGG + chr2_2:61238145-61238164 Msa0262370:CDS 50.0%
ATCCCTCCCATTCCACCAAA+GGG - chr2_2:61238150-61238169 None:intergenic 50.0%
TTTGGTGGAATGGGAGGGAT+GGG + chr2_2:61238150-61238169 Msa0262370:CDS 50.0%
ACTTCCTCCAGCATCACCAT+AGG - chr2_2:61238202-61238221 None:intergenic 50.0%
! TATGGTGATGCTGGAGGAAG+TGG + chr2_2:61238201-61238220 Msa0262370:CDS 50.0%
ACTAGAGGCAGAGGAAGACT+TGG + chr2_2:61238766-61238785 Msa0262370:intron 50.0%
!! ACTTAGTGGGGTTGGCTACA+TGG - chr2_2:61238872-61238891 None:intergenic 50.0%
GCAGAAGACTTCTACATCCC+TGG - chr2_2:61239818-61239837 None:intergenic 50.0%
GTGACTTTGCTGCACTCGAA+AGG - chr2_2:61240292-61240311 None:intergenic 50.0%
TTTGTCCCCTTCTTCCATCC+AGG - chr2_2:61240349-61240368 None:intergenic 50.0%
TCTCTTGCAGAAGCCTTAGC+TGG + chr2_2:61240499-61240518 Msa0262370:CDS 50.0%
GGAACAACCTCCTCATACTC+AGG - chr2_2:61240592-61240611 None:intergenic 50.0%
GAGGAGGTTGTTCCAAGAGA+AGG + chr2_2:61240598-61240617 Msa0262370:CDS 50.0%
AGAAGGAATGCCACTCCCAA+AGG + chr2_2:61240615-61240634 Msa0262370:CDS 50.0%
ATTCCCAACTCGGCTCACTT+CGG + chr2_2:61240681-61240700 Msa0262370:CDS 50.0%
AGAAGGGCAACAACCTGCCA+TGG + chr2_2:61237633-61237652 Msa0262370:exon 55.0%
GAAGGGCAACAACCTGCCAT+GGG + chr2_2:61237634-61237653 Msa0262370:exon 55.0%
! GCATGAGAATCAGGTGGTGG+TGG - chr2_2:61238021-61238040 None:intergenic 55.0%
GAGGCATGAGAATCAGGTGG+TGG - chr2_2:61238024-61238043 None:intergenic 55.0%
! CCAGAGGCATGAGAATCAGG+TGG - chr2_2:61238027-61238046 None:intergenic 55.0%
CCACCTGATTCTCATGCCTC+TGG + chr2_2:61238024-61238043 Msa0262370:CDS 55.0%
!! TCTCATGCCTCTGGTAGTGG+TGG + chr2_2:61238033-61238052 Msa0262370:CDS 55.0%
GGGGATCGAAATCCCTTTGG+TGG + chr2_2:61238135-61238154 Msa0262370:CDS 55.0%
CATCCCTCCCATTCCACCAA+AGG - chr2_2:61238151-61238170 None:intergenic 55.0%
CTTTGGTGGAATGGGAGGGA+TGG + chr2_2:61238149-61238168 Msa0262370:CDS 55.0%
GGGATGGGAAGAGGTAGCAA+TGG + chr2_2:61238165-61238184 Msa0262370:CDS 55.0%
! GGATCCTATGGTGATGCTGG+AGG + chr2_2:61238195-61238214 Msa0262370:CDS 55.0%
GCATCAAGGTCCTCCTCGTA+AGG + chr2_2:61238233-61238252 Msa0262370:CDS 55.0%
!! TCAATAGGAGGCGCCTTACG+AGG - chr2_2:61238249-61238268 None:intergenic 55.0%
GGAAGAGGAAGGTTCGACTG+CGG - chr2_2:61239119-61239138 None:intergenic 55.0%
!! GTCATGTCTGGTGTCGTGTC+CGG + chr2_2:61239429-61239448 Msa0262370:intron 55.0%
GTGCAGCAAAGTCACATCGC+TGG + chr2_2:61240297-61240316 Msa0262370:intron 55.0%
GGGAGTGGCATTCCTTCTCT+TGG - chr2_2:61240613-61240632 None:intergenic 55.0%
GATCCGAAGTGAGCCGAGTT+GGG - chr2_2:61240687-61240706 None:intergenic 55.0%
TGATCCGAAGTGAGCCGAGT+TGG - chr2_2:61240688-61240707 None:intergenic 55.0%
TCGGCTCACTTCGGATCAGA+AGG + chr2_2:61240690-61240709 Msa0262370:CDS 55.0%
CTCACTTCGGATCAGAAGGC+AGG + chr2_2:61240694-61240713 Msa0262370:CDS 55.0%
GGAATGGGAGGGATGGGAAG+AGG + chr2_2:61238156-61238175 Msa0262370:CDS 60.0%
!! ATAGGAGGCGCCTTACGAGG+AGG - chr2_2:61238246-61238265 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 61237496 61241069 61237496 ID=Msa0262370;Name=Msa0262370
chr2_2 mRNA 61237496 61241069 61237496 ID=Msa0262370-mRNA-1;Parent=Msa0262370;Name=Msa0262370-mRNA-1;_AED=0.06;_eAED=0.06;_QI=156|1|1|1|1|1|3|328|336
chr2_2 exon 61237496 61237816 61237496 ID=Msa0262370-mRNA-1:exon:18857;Parent=Msa0262370-mRNA-1
chr2_2 exon 61237949 61238355 61237949 ID=Msa0262370-mRNA-1:exon:18858;Parent=Msa0262370-mRNA-1
chr2_2 exon 61240303 61241069 61240303 ID=Msa0262370-mRNA-1:exon:18859;Parent=Msa0262370-mRNA-1
chr2_2 five_prime_UTR 61237496 61237651 61237496 ID=Msa0262370-mRNA-1:five_prime_utr;Parent=Msa0262370-mRNA-1
chr2_2 CDS 61237652 61237816 61237652 ID=Msa0262370-mRNA-1:cds;Parent=Msa0262370-mRNA-1
chr2_2 CDS 61237949 61238355 61237949 ID=Msa0262370-mRNA-1:cds;Parent=Msa0262370-mRNA-1
chr2_2 CDS 61240303 61240741 61240303 ID=Msa0262370-mRNA-1:cds;Parent=Msa0262370-mRNA-1
chr2_2 three_prime_UTR 61240742 61241069 61240742 ID=Msa0262370-mRNA-1:three_prime_utr;Parent=Msa0262370-mRNA-1
Gene Sequence

>Msa0262370

ATGGGTGTTGACTACTACAAGCTTCTTCAGGTTGATCGGAACGCCAAAGACGATGACTTGAAGAAAGCTTACAGAAAACTCGCCATGAAGTGGCATCCTGATAAGAACCCTAATAACAAAAAAGAAGCTGAAGCCAAATTTAAGCAAATCTCAGAAGCTTATGATGTATTGAGTGATTCTAACAAGAGAGCTGTTTATGATCAATATGGGGAAGATGGGCTGAAGGGTCAAATGCCACCACCACCTGATTCTCATGCCTCTGGTAGTGGTGGTAGTAGAAACTTTTCACCTGATGACTTTCCGTATCAGTTCAATCATCGGACTCCTGATGATATTTTTGCTGAGTTTTTTGGGGATCGAAATCCCTTTGGTGGAATGGGAGGGATGGGAAGAGGTAGCAATGGCATGTTTGGATCCTATGGTGATGCTGGAGGAAGTGGAGTCAATGTGCATCAAGGTCCTCCTCGTAAGGCGCCTCCTATTGAGAACAAGTTGCTATGTTCTCTTGAGGATATATATAAAGGGACCACTAAGAAGATGAAGATCACCAGAGAAATTCTTGATCACAGTGGCAAAGTCACATCGCTGGATGAAATCTTGACAATTAGTGTCAAACCTGGATGGAAGAAGGGGACAAAGATCACTTTCCCCGAGAAGGGAAATGAACATCCAAATACCATACCTGCTGATATCATTTTCATCATCGATGAAAAACCACACAATGTGTTCACTCGTGAAGGAAATGATCTAATTGTAACTCAGAAAATTTCTCTTGCAGAAGCCTTAGCTGGTTGTACAGTGAATCTCACTACACTTGATGGCAGAAATTTGACTGTAGTGATCAACAATGTTGTTCATCCTGAGTATGAGGAGGTTGTTCCAAGAGAAGGAATGCCACTCCCAAAGGATCCAACAAAGAAGGGAAACTTGAGGATAAAGTTCAACGTCAAATTCCCAACTCGGCTCACTTCGGATCAGAAGGCAGGAATGAAGAAAGTCTTGGCTGGATAA
Protein sequence

>Msa0262370

MGVDYYKLLQVDRNAKDDDLKKAYRKLAMKWHPDKNPNNKKEAEAKFKQISEAYDVLSDSNKRAVYDQYGEDGLKGQMPPPPDSHASGSGGSRNFSPDDFPYQFNHRTPDDIFAEFFGDRNPFGGMGGMGRGSNGMFGSYGDAGGSGVNVHQGPPRKAPPIENKLLCSLEDIYKGTTKKMKITREILDHSGKVTSLDEILTISVKPGWKKGTKITFPEKGNEHPNTIPADIIFIIDEKPHNVFTREGNDLIVTQKISLAEALAGCTVNLTTLDGRNLTVVINNVVHPEYEEVVPREGMPLPKDPTKKGNLRIKFNVKFPTRLTSDQKAGMKKVLAG*