AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0337590

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0337590 MtrunA17_Chr2g0324921 97.619 336 8 0 1 336 1 336 0.0 680
Msa0337590 MtrunA17_Chr4g0040501 68.362 354 83 5 1 335 1 344 2.13e-173 485
Msa0337590 MtrunA17_Chr2g0282741 66.476 349 93 4 1 335 1 339 1.13e-162 457
Msa0337590 MtrunA17_Chr4g0006391 65.330 349 99 6 1 336 1 340 1.57e-157 444
Msa0337590 MtrunA17_Chr2g0282761 56.196 347 125 5 1 328 1 339 3.71e-131 377
Msa0337590 MtrunA17_Chr3g0138471 54.783 345 129 5 1 336 1 327 9.45e-125 360
Msa0337590 MtrunA17_Chr3g0093111 43.750 336 161 3 3 335 1 311 3.68e-94 282
Msa0337590 MtrunA17_Chr5g0415751 59.310 145 59 0 190 334 16 160 2.36e-57 183
Msa0337590 MtrunA17_Chr3g0124801 44.693 179 98 1 157 334 113 291 5.68e-50 168
Msa0337590 MtrunA17_Chr7g0269841 34.808 339 190 8 5 334 27 343 2.14e-49 168
Msa0337590 MtrunA17_Chr2g0277721 42.778 180 102 1 156 334 80 259 1.58e-46 158
Msa0337590 MtrunA17_Chr8g0334811 31.313 396 158 11 5 334 15 362 5.00e-34 129
Msa0337590 MtrunA17_Chr2g0322181 40.690 145 84 1 192 334 214 358 1.17e-28 114
Msa0337590 MtrunA17_Chr2g0322181 57.746 71 25 1 5 75 14 79 1.54e-11 65.1
Msa0337590 MtrunA17_Chr4g0025401 40.690 145 84 1 192 334 212 356 9.03e-28 112
Msa0337590 MtrunA17_Chr3g0091351 50.000 96 48 0 220 315 2 97 6.55e-26 99.8
Msa0337590 MtrunA17_Chr5g0426951 27.933 358 210 12 4 332 83 421 1.37e-23 100
Msa0337590 MtrunA17_Chr6g0478651 35.714 182 78 5 2 183 66 208 1.45e-21 95.5
Msa0337590 MtrunA17_Chr3g0087741 28.000 350 212 9 1 327 86 418 5.12e-21 93.2
Msa0337590 MtrunA17_Chr7g0263971 56.522 69 29 1 4 72 369 436 3.63e-20 91.3
Msa0337590 MtrunA17_Chr4g0010781 25.843 356 215 9 1 327 83 418 6.46e-20 90.1
Msa0337590 MtrunA17_Chr5g0415731 56.000 75 26 1 4 71 5 79 1.49e-19 84.0
Msa0337590 MtrunA17_Chr2g0333221 42.609 115 44 3 4 118 70 162 7.83e-18 84.3
Msa0337590 MtrunA17_Chr3g0123861 54.795 73 31 1 3 75 81 151 1.18e-17 83.6
Msa0337590 MtrunA17_Chr7g0271891 53.030 66 28 1 5 67 17 82 3.51e-17 77.8
Msa0337590 MtrunA17_Chr4g0021141 50.746 67 32 1 4 70 22 87 2.24e-15 72.0
Msa0337590 MtrunA17_Chr8g0344721 49.275 69 30 1 4 67 10 78 4.80e-15 73.6
Msa0337590 MtrunA17_Chr5g0399201 45.946 74 38 1 4 75 12 85 1.98e-14 73.9
Msa0337590 MtrunA17_Chr5g0427221 51.562 64 30 1 4 67 48 110 2.93e-14 69.3
Msa0337590 MtrunA17_Chr2g0295821 36.641 131 59 4 4 125 104 219 8.44e-14 71.6
Msa0337590 MtrunA17_Chr1g0159491 46.377 69 36 1 3 71 5 72 1.51e-13 65.5
Msa0337590 MtrunA17_Chr1g0209551 47.887 71 36 1 4 74 16 85 2.94e-13 70.1
Msa0337590 MtrunA17_Chr7g0242951 47.143 70 35 1 3 72 26 93 3.35e-13 70.5
Msa0337590 MtrunA17_Chr4g0044331 50.000 60 27 1 11 67 19 78 6.26e-13 65.9
Msa0337590 MtrunA17_Chr5g0414471 46.575 73 38 1 4 76 25 96 1.17e-12 68.6
Msa0337590 MtrunA17_Chr1g0173931 47.692 65 33 1 6 70 40 103 2.97e-12 66.2
Msa0337590 MtrunA17_Chr6g0460461 37.895 95 41 2 3 93 1 81 2.98e-12 65.1
Msa0337590 MtrunA17_Chr6g0460411 37.895 95 41 2 3 93 1 81 3.27e-12 65.1
Msa0337590 MtrunA17_Chr7g0258061 44.595 74 37 2 6 76 24 96 9.94e-12 64.7
Msa0337590 MtrunA17_Chr6g0460381 42.029 69 36 1 3 67 2 70 3.05e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0337590 AT2G20560.1 71.637 342 83 3 1 334 1 336 1.20e-165 465
Msa0337590 AT4G28480.1 69.801 351 85 6 1 334 1 347 8.74e-157 443
Msa0337590 AT3G08910.1 65.476 336 102 4 1 336 1 322 1.23e-154 437
Msa0337590 AT5G01390.1 59.710 345 118 5 1 335 1 334 3.22e-142 405
Msa0337590 AT5G25530.2 55.000 360 117 6 1 336 1 339 3.31e-134 385
Msa0337590 AT5G25530.1 53.804 368 117 7 1 336 1 347 2.33e-131 379
Msa0337590 AT1G10350.1 52.571 350 150 5 1 336 1 348 1.90e-124 361
Msa0337590 AT3G47940.1 54.000 350 145 4 1 336 1 348 2.04e-119 348
Msa0337590 AT1G59725.1 55.786 337 141 4 1 336 1 330 3.65e-119 347
Msa0337590 AT5G01390.2 53.550 338 92 5 1 335 1 276 3.59e-116 337
Msa0337590 AT4G28480.2 55.425 341 93 10 1 334 1 289 4.03e-99 295
Msa0337590 AT2G20550.1 58.242 273 85 4 84 334 18 283 6.58e-92 276
Msa0337590 AT2G20550.2 58.242 273 85 4 84 334 18 283 6.58e-92 276
Msa0337590 AT5G01390.3 45.562 338 80 6 1 335 1 237 4.15e-86 259
Msa0337590 AT5G01390.4 54.369 206 73 4 1 196 1 195 8.84e-64 202
Msa0337590 AT1G44160.1 46.629 178 95 0 157 334 176 353 9.77e-50 170
Msa0337590 AT1G11040.1 40.331 181 108 0 154 334 252 432 1.16e-45 161
Msa0337590 AT3G62600.1 34.682 346 191 9 2 335 24 346 6.96e-45 157
Msa0337590 AT5G22060.1 32.800 375 177 10 5 334 15 359 5.52e-39 143
Msa0337590 AT3G44110.1 33.428 353 203 8 5 334 15 358 2.17e-38 141
Msa0337590 AT3G44110.2 32.515 326 188 8 5 307 15 331 2.38e-30 118
Msa0337590 AT5G48030.1 26.816 358 209 13 4 327 94 432 2.30e-22 97.8
Msa0337590 AT1G80030.4 43.651 126 47 5 4 129 75 176 4.03e-19 88.6
Msa0337590 AT1G80030.2 43.651 126 47 5 4 129 75 176 5.20e-19 88.2
Msa0337590 AT1G80030.3 43.651 126 47 5 4 129 75 176 5.20e-19 88.2
Msa0337590 AT1G80030.1 43.651 126 47 5 4 129 75 176 5.20e-19 88.2
Msa0337590 AT2G22360.1 55.405 74 31 1 3 76 85 156 6.32e-18 84.7
Msa0337590 AT4G39960.1 41.228 114 44 3 4 117 85 175 1.51e-17 83.6
Msa0337590 AT4G39960.2 41.228 114 44 3 4 117 85 175 1.51e-17 83.6
Msa0337590 AT5G03160.1 53.623 69 31 1 4 72 370 437 2.82e-17 82.8
Msa0337590 AT1G56300.2 53.030 66 28 1 5 67 14 79 8.54e-17 75.9
Msa0337590 AT1G56300.1 53.030 66 28 1 5 67 14 79 9.86e-17 76.6
Msa0337590 AT2G35720.1 41.975 81 45 1 4 82 15 95 3.61e-14 73.6
Msa0337590 AT5G16650.2 50.746 67 32 1 4 70 11 76 5.96e-14 69.3
Msa0337590 AT5G16650.1 51.562 64 30 1 4 67 11 73 7.48e-14 68.2
Msa0337590 AT2G41000.2 46.377 69 33 1 3 67 2 70 3.76e-13 68.2
Msa0337590 AT3G17830.1 38.849 139 51 3 2 140 61 165 5.26e-13 70.1
Msa0337590 AT1G68370.1 47.887 71 36 1 4 74 17 86 8.51e-13 69.3
Msa0337590 AT2G41000.1 46.377 69 33 1 3 67 2 70 9.58e-13 66.2
Msa0337590 AT3G08970.1 48.000 75 35 2 3 77 26 96 1.02e-12 69.3
Msa0337590 AT3G08970.2 48.000 75 35 2 3 77 26 96 1.02e-12 69.3
Msa0337590 AT5G05750.1 34.266 143 73 4 4 146 114 235 1.04e-12 68.2
Msa0337590 AT5G06910.1 50.769 65 31 1 6 70 31 94 1.12e-12 67.8
Msa0337590 AT1G71000.2 46.970 66 32 1 5 67 9 74 3.21e-12 64.3
Msa0337590 AT2G21510.3 47.222 72 37 1 4 75 6 76 3.43e-12 67.0
Msa0337590 AT2G21510.1 47.222 72 37 1 4 75 6 76 3.43e-12 67.0
Msa0337590 AT1G59980.2 42.029 69 39 1 6 74 25 92 3.64e-12 67.0
Msa0337590 AT2G21510.2 47.222 72 37 1 4 75 6 76 3.94e-12 67.0
Msa0337590 AT2G21510.4 47.222 72 37 1 4 75 6 76 3.94e-12 67.0
Msa0337590 AT2G21510.5 47.222 72 37 1 4 75 6 76 5.18e-12 66.2
Msa0337590 AT1G71000.1 46.970 66 32 1 5 67 9 74 5.34e-12 63.9
Msa0337590 AT1G59980.1 42.029 69 39 1 6 74 25 92 6.42e-12 66.6
Msa0337590 AT3G14200.1 46.875 64 32 1 6 67 14 77 7.97e-12 64.7
Msa0337590 AT1G72416.3 45.455 66 34 1 4 67 5 70 1.46e-11 63.5
Msa0337590 AT5G49060.2 33.913 115 67 2 4 118 99 204 1.91e-11 64.7
Msa0337590 AT5G49060.1 33.913 115 67 2 4 118 99 204 1.91e-11 64.7
Msa0337590 AT1G72416.4 45.455 66 34 1 4 67 5 70 1.97e-11 62.8
Msa0337590 AT1G72416.1 45.455 66 34 1 4 67 5 70 2.07e-11 62.8
Msa0337590 AT5G37750.1 39.130 92 54 1 4 95 70 159 2.16e-11 63.5
Msa0337590 AT1G72416.2 45.455 66 34 1 4 67 5 70 2.71e-11 62.4
Msa0337590 AT2G33735.1 43.077 65 36 1 4 68 22 85 3.00e-11 60.5
Msa0337590 AT2G33735.2 43.077 65 36 1 4 68 22 85 3.00e-11 60.5
Msa0337590 AT1G24120.1 41.892 74 42 1 4 77 20 92 3.47e-11 64.3
Msa0337590 AT4G19570.1 51.515 66 30 1 3 68 65 128 4.16e-11 64.3

Find 123 sgRNAs with CRISPR-Local

Find 271 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACAAATATATGAATGTTTA+TGG 0.125821 2_4:+57628861 None:intergenic
CCCTTCTTTGTTGGATCCTT+TGG 0.129787 2_4:+57629278 None:intergenic
GTTAATGTTACATTAAAATT+TGG 0.135986 2_4:-57628951 Msa0337590:three_prime_UTR
CAGGAGGCAATTGGGAAATT+TGG 0.163872 2_4:-57629091 Msa0337590:three_prime_UTR
TTCTGAGATTTGCTTAAATT+TGG 0.199073 2_4:+57632131 None:intergenic
CTCAAGTTTCCCTTCTTTGT+TGG 0.262625 2_4:+57629269 None:intergenic
ATATCAGCAGGTATGGTATT+TGG 0.272102 2_4:+57629509 None:intergenic
CCTTCTTTGTTGGATCCTTT+GGG 0.278902 2_4:+57629279 None:intergenic
ATTGGGAAATTTGGTTGTTA+TGG 0.283905 2_4:-57629082 Msa0337590:three_prime_UTR
AGAGCTGTTTATGATCAATA+TGG 0.297643 2_4:-57631946 Msa0337590:CDS
TCTCTTGAGGATATATATAA+AGG 0.310218 2_4:-57631631 Msa0337590:CDS
ACCAACATTATTCAACTTCA+TGG 0.313695 2_4:-57628915 Msa0337590:three_prime_UTR
TGGATGTTCATTTCCCTTCT+CGG 0.321746 2_4:+57629529 None:intergenic
TTTCTTCAAGTCATCGTCTT+TGG 0.326757 2_4:+57632221 None:intergenic
ATTGCCTCCTGTCAAGATTA+TGG 0.339096 2_4:+57629103 None:intergenic
GGATAAAGTAAGATATTAAT+TGG 0.350954 2_4:-57629173 Msa0337590:exon
TTGTTATTAGGGTTCTTATC+AGG 0.352145 2_4:+57632168 None:intergenic
GGGAGTGGCATTCCTTCTCT+TGG 0.354044 2_4:+57629299 None:intergenic
AGAGGTAGCAATGGCATGTT+TGG 0.355428 2_4:-57631742 Msa0337590:CDS
GGTGATCTTCATCTTCTTAG+TGG 0.355739 2_4:+57631606 None:intergenic
GGATGTTCATTTCCCTTCTC+GGG 0.357845 2_4:+57629530 None:intergenic
TTAATGTTACATTAAAATTT+GGG 0.367373 2_4:-57628950 Msa0337590:three_prime_UTR
TGACTACTACAAGCTTCTTC+AGG 0.376848 2_4:-57632256 Msa0337590:CDS
TAATCTTGACAGGAGGCAAT+TGG 0.388489 2_4:-57629100 Msa0337590:three_prime_UTR
CTACCAGAGGCATGAGAATC+AGG 0.390225 2_4:+57631889 None:intergenic
GCCGCAAAATCGCAGAAAAT+CGG 0.395524 2_4:-57632343 Msa0337590:five_prime_UTR
TTTGTCCCCTTCTTCCATCC+AGG 0.403890 2_4:+57629563 None:intergenic
GGAATGGGAGGGATGGGAAG+AGG 0.407566 2_4:-57631760 Msa0337590:CDS
CGAAATCCCTTTGGTGGAAT+GGG 0.411716 2_4:-57631775 Msa0337590:CDS
CTTATCAGGATGCCACTTCA+TGG 0.411951 2_4:+57632182 None:intergenic
CATAGCAACTTGTTCTCAAT+AGG 0.424637 2_4:+57631655 None:intergenic
CATTATTCAACTTCATGGTA+TGG 0.433834 2_4:-57628910 Msa0337590:three_prime_UTR
GAGCTGTTTATGATCAATAT+GGG 0.439767 2_4:-57631945 Msa0337590:CDS
GTGAATCTCACTACACTTGA+TGG 0.443477 2_4:-57629380 Msa0337590:CDS
TTTGGGGATCGAAATCCCTT+TGG 0.447674 2_4:-57631784 Msa0337590:CDS
TATGATCAATATGGGGAAGA+TGG 0.451224 2_4:-57631937 Msa0337590:CDS
GATTCTCATGCCTCTGGTAG+TGG 0.458632 2_4:-57631886 Msa0337590:CDS
TCGAAATCCCTTTGGTGGAA+TGG 0.459463 2_4:-57631776 Msa0337590:CDS
TCTCTTGCAGAAGCCTTAGT+TGG 0.462639 2_4:-57629410 Msa0337590:CDS
ATTATTCAACTTCATGGTAT+GGG 0.463867 2_4:-57628909 Msa0337590:three_prime_UTR
TTTGTTGGATCCTTTGGGAG+TGG 0.464104 2_4:+57629284 None:intergenic
AACTGATACGGAAAGTCATC+AGG 0.465543 2_4:+57631844 None:intergenic
GTTAGACAATGAGTTACATT+GGG 0.467007 2_4:-57628991 Msa0337590:three_prime_UTR
CTCTTGAGGATATATATAAA+GGG 0.467907 2_4:-57631630 Msa0337590:CDS
GATCCGAAGTGAGCCGAGTT+GGG 0.468185 2_4:+57629225 None:intergenic
CCCAAAGGATCCAACAAAGA+AGG 0.480916 2_4:-57629279 Msa0337590:CDS
GGGGATCGAAATCCCTTTGG+TGG 0.482474 2_4:-57631781 Msa0337590:CDS
AATCTTGACAGGAGGCAATT+GGG 0.488582 2_4:-57629099 Msa0337590:three_prime_UTR
TAGATTACCATAATCTTGAC+AGG 0.489863 2_4:-57629110 Msa0337590:three_prime_UTR
GTCAAACCTGGATGGAAGAA+GGG 0.491815 2_4:-57629569 Msa0337590:CDS
GAAAATGATATCAGCAGGTA+TGG 0.491993 2_4:+57629502 None:intergenic
TATGGTGATGCTGGAGGAAG+TGG 0.494603 2_4:-57631715 Msa0337590:CDS
TGATCCGAAGTGAGCCGAGT+TGG 0.495068 2_4:+57629224 None:intergenic
TGTTGTTCATCCTGAGTATG+AGG 0.496005 2_4:-57629330 Msa0337590:CDS
ACTGTGATCAAGAATTTCTC+TGG 0.501571 2_4:+57631585 None:intergenic
CTTTGGTGGAATGGGAGGGA+TGG 0.502219 2_4:-57631767 Msa0337590:CDS
TTTGGTGGAATGGGAGGGAT+GGG 0.503447 2_4:-57631766 Msa0337590:CDS
TGTCAAACCTGGATGGAAGA+AGG 0.504580 2_4:-57629570 Msa0337590:CDS
TTTGGATCCTATGGTGATGC+TGG 0.505971 2_4:-57631724 Msa0337590:CDS
GGTTAGACAATGAGTTACAT+TGG 0.506915 2_4:-57628992 Msa0337590:three_prime_UTR
ATCCCTCCCATTCCACCAAA+GGG 0.515203 2_4:+57631769 None:intergenic
ACAAGCTTCTTCAGGTTGAT+CGG 0.516804 2_4:-57632248 Msa0337590:CDS
GTCCGATGATTGAACTGATA+CGG 0.527889 2_4:+57631832 None:intergenic
ATGATCAATATGGGGAAGAT+GGG 0.529413 2_4:-57631936 Msa0337590:CDS
CCACCTGATTCTCATGCCTC+TGG 0.530969 2_4:-57631892 Msa0337590:CDS
AATGGCATGTTTGGATCCTA+TGG 0.531436 2_4:-57631733 Msa0337590:CDS
ACCATGAAGTTGAATAATGT+TGG 0.535476 2_4:+57628914 None:intergenic
AGAAGGGCAACAACCTGCCA+TGG 0.538305 2_4:-57632283 Msa0337590:exon
ATTCACTGTACAACCAACTA+AGG 0.541106 2_4:+57629397 None:intergenic
ATGATGAAAATGATATCAGC+AGG 0.541292 2_4:+57629497 None:intergenic
GCATGAGAATCAGGTGGTGG+TGG 0.542892 2_4:+57631898 None:intergenic
AAAGATCACTTTCCCCGAGA+AGG 0.545291 2_4:-57629543 Msa0337590:CDS
CATCCCTCCCATTCCACCAA+AGG 0.550801 2_4:+57631768 None:intergenic
ATATGGGGAAGATGGGCTGA+AGG 0.551422 2_4:-57631929 Msa0337590:CDS
CTCACTTCGGATCAGAAAGC+AGG 0.551807 2_4:-57629215 Msa0337590:CDS
GGAATGAAGAAAGTCTTGGC+TGG 0.552067 2_4:-57629194 Msa0337590:CDS
AGCAGGAATGAAGAAAGTCT+TGG 0.552760 2_4:-57629198 Msa0337590:CDS
CTGTCGAAGAGAAAGAGAGA+AGG 0.555619 2_4:-57632300 Msa0337590:five_prime_UTR
TTCCGTATCAGTTCAATCAT+CGG 0.556341 2_4:-57631834 Msa0337590:CDS
GGAACAACCTCCTCATACTC+AGG 0.557628 2_4:+57629320 None:intergenic
GTGCAGCAAAGTCACATCGC+TGG 0.559149 2_4:-57629612 Msa0337590:intron
GCATCAAGGTCCTCCTCGTA+AGG 0.559593 2_4:-57631683 Msa0337590:CDS
ACAGAAAACTCGCCATGAAG+TGG 0.563345 2_4:-57632194 Msa0337590:CDS
AAGATCACTTTCCCCGAGAA+GGG 0.565073 2_4:-57629542 Msa0337590:CDS
CACAATGTGTTCACTCGTGA+AGG 0.567306 2_4:-57629461 Msa0337590:CDS
CCAAAGGATCCAACAAAGAA+GGG 0.568075 2_4:-57629278 Msa0337590:CDS
AGCAACTTGTTCTCAATAGG+AGG 0.568821 2_4:+57631658 None:intergenic
TGTCGAAGAGAAAGAGAGAA+GGG 0.570583 2_4:-57632299 Msa0337590:five_prime_UTR
TAGTAGTCAACACCCATGGC+AGG 0.572331 2_4:+57632270 None:intergenic
AATCCCTTTGGTGGAATGGG+AGG 0.575318 2_4:-57631772 Msa0337590:CDS
CAATTAGTGTCAAACCTGGA+TGG 0.581052 2_4:-57629577 Msa0337590:CDS
ATTCCCAACTCGGCTCACTT+CGG 0.581525 2_4:-57629228 Msa0337590:CDS
GAGAAATTCTTGATCACAGT+GGG 0.581668 2_4:-57631582 Msa0337590:intron
TCACGAGTGAACACATTGTG+TGG 0.583017 2_4:+57629464 None:intergenic
ACTTCCTCCAGCATCACCAT+AGG 0.584524 2_4:+57631717 None:intergenic
TATGGGGAAGATGGGCTGAA+GGG 0.585281 2_4:-57631928 Msa0337590:CDS
TTGACAATTAGTGTCAAACC+TGG 0.594845 2_4:-57629581 Msa0337590:CDS
AGTGGAGTCAATGTGCATCA+AGG 0.595190 2_4:-57631697 Msa0337590:CDS
TCTCATGCCTCTGGTAGTGG+TGG 0.595916 2_4:-57631883 Msa0337590:CDS
ATCCCTTTGGTGGAATGGGA+GGG 0.596841 2_4:-57631771 Msa0337590:CDS
TTATTCAACTTCATGGTATG+GGG 0.599396 2_4:-57628908 Msa0337590:three_prime_UTR
GAAGGGCAACAACCTGCCAT+GGG 0.605250 2_4:-57632282 Msa0337590:exon
GCTTAGGTACAATACAATCG+TGG 0.609230 2_4:-57632423 Msa0337590:five_prime_UTR
GAGGAGGTTGTTCCAAGAGA+AGG 0.609654 2_4:-57629311 Msa0337590:CDS
GAGGCATGAGAATCAGGTGG+TGG 0.609693 2_4:+57631895 None:intergenic
AGAAGGAATGCCACTCCCAA+AGG 0.611731 2_4:-57629294 Msa0337590:CDS
GATGTTCATTTCCCTTCTCG+GGG 0.615186 2_4:+57629531 None:intergenic
CTTAGGTACAATACAATCGT+GGG 0.615629 2_4:-57632422 Msa0337590:five_prime_UTR
TCAAACCTGGATGGAAGAAG+GGG 0.618407 2_4:-57629568 Msa0337590:CDS
TCAATAGGAGGCGCCTTACG+AGG 0.622058 2_4:+57631670 None:intergenic
TCAACGTCAAATTCCCAACT+CGG 0.622716 2_4:-57629238 Msa0337590:CDS
CAAGTTGCTATGTTCTCTTG+AGG 0.636918 2_4:-57631644 Msa0337590:CDS
GGGATGGGAAGAGGTAGCAA+TGG 0.641843 2_4:-57631751 Msa0337590:CDS
CAACAAAGAAGGGAAACTTG+AGG 0.650514 2_4:-57629268 Msa0337590:CDS
GGATCCTATGGTGATGCTGG+AGG 0.665018 2_4:-57631721 Msa0337590:CDS
ATTACCATAATCTTGACAGG+AGG 0.665326 2_4:-57629107 Msa0337590:three_prime_UTR
CTTGTAGTAGTCAACACCCA+TGG 0.668721 2_4:+57632266 None:intergenic
ATAGGAGGCGCCTTACGAGG+AGG 0.673195 2_4:+57631673 None:intergenic
AGCTGTTTATGATCAATATG+GGG 0.674859 2_4:-57631944 Msa0337590:CDS
TCTACTACCACCACTACCAG+AGG 0.706463 2_4:+57631876 None:intergenic
CCAGAGGCATGAGAATCAGG+TGG 0.707871 2_4:+57631892 None:intergenic
TGTTCATCCTGAGTATGAGG+AGG 0.716066 2_4:-57629327 Msa0337590:CDS
AGAGAAATTCTTGATCACAG+TGG 0.741173 2_4:-57631583 Msa0337590:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATAGAACTTTTTTTTTTTT+TGG - chr2_4:57630585-57630604 Msa0337590:intron 10.0%
!!! ATAGAACTTTTTTTTTTTTT+GGG - chr2_4:57630586-57630605 Msa0337590:intron 10.0%
!!! ATTTAAAATTTTGAAAAAGT+GGG + chr2_4:57630748-57630767 None:intergenic 10.0%
!!! AATTTAAAATTTTGAAAAAG+TGG + chr2_4:57630749-57630768 None:intergenic 10.0%
!! TTAATGTTACATTAAAATTT+GGG - chr2_4:57632331-57632350 Msa0337590:five_prime_UTR 10.0%
!!! ATGATGTAAGTTTTTTTTAA+AGG - chr2_4:57629177-57629196 Msa0337590:exon 15.0%
!!! TGATGTAAGTTTTTTTTAAA+GGG - chr2_4:57629178-57629197 Msa0337590:exon 15.0%
!!! CTTAAATTTTCAATTTTTAG+TGG - chr2_4:57629204-57629223 Msa0337590:CDS 15.0%
!! TATAAATATCTAATAACTGA+TGG + chr2_4:57629776-57629795 None:intergenic 15.0%
!! TAAACAATTACTATAATTCT+AGG + chr2_4:57630434-57630453 None:intergenic 15.0%
!! TATAAATCAACATTATGATA+TGG + chr2_4:57631005-57631024 None:intergenic 15.0%
!!! TAGTATAGTAATCTATTTTT+TGG - chr2_4:57632239-57632258 Msa0337590:CDS 15.0%
!!! TATTTTTTGGAATGATATTT+TGG - chr2_4:57632252-57632271 Msa0337590:CDS 15.0%
!! GTTAATGTTACATTAAAATT+TGG - chr2_4:57632330-57632349 Msa0337590:five_prime_UTR 15.0%
!!! GGAATTTGTTTTTTGTTATT+AGG - chr2_4:57629292-57629311 Msa0337590:CDS 20.0%
!!! ATATTTTTGCTGAGTTTTTT+GGG - chr2_4:57629480-57629499 Msa0337590:CDS 20.0%
!! TCATAATATTAGATAGATAG+TGG - chr2_4:57629984-57630003 Msa0337590:intron 20.0%
!! CATAATATTAGATAGATAGT+GGG - chr2_4:57629985-57630004 Msa0337590:intron 20.0%
!! ATAATATTAGATAGATAGTG+GGG - chr2_4:57629986-57630005 Msa0337590:intron 20.0%
!! GTTCTATTAGAAAAAATTCA+AGG + chr2_4:57630573-57630592 None:intergenic 20.0%
!!! AACTTTTTTTTTTTTTGGGT+AGG - chr2_4:57630590-57630609 Msa0337590:intron 20.0%
!! TTGATTTATAGTAGTATGTT+TGG - chr2_4:57631015-57631034 Msa0337590:intron 20.0%
!! TGATTTATAGTAGTATGTTT+GGG - chr2_4:57631016-57631035 Msa0337590:intron 20.0%
!! TTTAATGTTGCAAATTACAA+TGG - chr2_4:57631260-57631279 Msa0337590:intron 20.0%
!!! TTGATGTTTTTCTAGTATAA+TGG - chr2_4:57631487-57631506 Msa0337590:intron 20.0%
!! ATTCTAGATGTTTAAATTAG+AGG + chr2_4:57631540-57631559 None:intergenic 20.0%
!! AAAAAACAAAGTCATCAATA+GGG + chr2_4:57631635-57631654 None:intergenic 20.0%
!! AAAAAAACAAAGTCATCAAT+AGG + chr2_4:57631636-57631655 None:intergenic 20.0%
!! GGATAAAGTAAGATATTAAT+TGG - chr2_4:57632108-57632127 Msa0337590:intron 20.0%
!!! TGATATTTTGGTCTATTTTA+TGG - chr2_4:57632264-57632283 Msa0337590:CDS 20.0%
!! CACAAATATATGAATGTTTA+TGG + chr2_4:57632423-57632442 None:intergenic 20.0%
!!! GTTCAAGAAATTTTAACGTT+GGG + chr2_4:57628899-57628918 None:intergenic 25.0%
!!! AGTTCAAGAAATTTTAACGT+TGG + chr2_4:57628900-57628919 None:intergenic 25.0%
!!! CAGCTTCTTTTTTGTTATTA+GGG + chr2_4:57629127-57629146 None:intergenic 25.0%
!!! TCAGCTTCTTTTTTGTTATT+AGG + chr2_4:57629128-57629147 None:intergenic 25.0%
!!! TTCTGAGATTTGCTTAAATT+TGG + chr2_4:57629153-57629172 None:intergenic 25.0%
!!! AAAGTTTTGTTTTTTGCATC+TGG - chr2_4:57629249-57629268 Msa0337590:CDS 25.0%
!!! AAGTTTTGTTTTTTGCATCT+GGG - chr2_4:57629250-57629269 Msa0337590:CDS 25.0%
!!! GATATTTTTGCTGAGTTTTT+TGG - chr2_4:57629479-57629498 Msa0337590:CDS 25.0%
!!! TATTTTTGCTGAGTTTTTTG+GGG - chr2_4:57629481-57629500 Msa0337590:CDS 25.0%
! TCTCTTGAGGATATATATAA+AGG - chr2_4:57629650-57629669 Msa0337590:intron 25.0%
! CTCTTGAGGATATATATAAA+GGG - chr2_4:57629651-57629670 Msa0337590:intron 25.0%
!! TAATATTAGATAGATAGTGG+GGG - chr2_4:57629987-57630006 Msa0337590:intron 25.0%
! TAATGAGTTGTATCGAAATA+TGG - chr2_4:57630193-57630212 Msa0337590:intron 25.0%
! TTGTATCGAAATATGGTTTA+TGG - chr2_4:57630200-57630219 Msa0337590:intron 25.0%
! GAACCATCAATACTTAAAAT+TGG + chr2_4:57630508-57630527 None:intergenic 25.0%
! CGAGTTATATTGGTATAAAT+TGG - chr2_4:57630858-57630877 Msa0337590:intron 25.0%
! GATTTATAGTAGTATGTTTG+GGG - chr2_4:57631017-57631036 Msa0337590:intron 25.0%
! ATTTATAGTAGTATGTTTGG+GGG - chr2_4:57631018-57631037 Msa0337590:intron 25.0%
!!! TCTGGTTTTTGAGATTATAT+TGG - chr2_4:57631145-57631164 Msa0337590:intron 25.0%
! TTGTAACATTAAACAAGAGA+AGG + chr2_4:57631350-57631369 None:intergenic 25.0%
!! TCTCTTGTTTAATGTTACAA+CGG - chr2_4:57631350-57631369 Msa0337590:intron 25.0%
!! TAAACTGTTGTTATTGCTAT+AGG - chr2_4:57631460-57631479 Msa0337590:intron 25.0%
!! AAACTGTTGTTATTGCTATA+GGG - chr2_4:57631461-57631480 Msa0337590:intron 25.0%
! GATATTAATACAGACTAACA+TGG + chr2_4:57631518-57631537 None:intergenic 25.0%
!! ATTAATTGGTACATCTTTTG+AGG - chr2_4:57632122-57632141 Msa0337590:CDS 25.0%
!!! ATTTTGGTCTATTTTATGGT+TGG - chr2_4:57632268-57632287 Msa0337590:exon 25.0%
! ATTATTCAACTTCATGGTAT+GGG - chr2_4:57632372-57632391 Msa0337590:five_prime_UTR 25.0%
! TTGTTATTAGGGTTCTTATC+AGG + chr2_4:57629116-57629135 None:intergenic 30.0%
AGAGCTGTTTATGATCAATA+TGG - chr2_4:57629335-57629354 Msa0337590:CDS 30.0%
GAGCTGTTTATGATCAATAT+GGG - chr2_4:57629336-57629355 Msa0337590:CDS 30.0%
AGCTGTTTATGATCAATATG+GGG - chr2_4:57629337-57629356 Msa0337590:CDS 30.0%
AACTCAGCAAAAATATCATC+AGG + chr2_4:57629476-57629495 None:intergenic 30.0%
! ACAGCATTGAAAAAAGAACT+AGG + chr2_4:57629745-57629764 None:intergenic 30.0%
ATTGCTGAGATTTACATGTT+TGG - chr2_4:57629835-57629854 Msa0337590:intron 30.0%
! TGTAGCATTTCTAGATTTAC+AGG - chr2_4:57629872-57629891 Msa0337590:intron 30.0%
AAGTATTATCTCACTTAGTG+GGG + chr2_4:57630249-57630268 None:intergenic 30.0%
CAAGTATTATCTCACTTAGT+GGG + chr2_4:57630250-57630269 None:intergenic 30.0%
ACAAGTATTATCTCACTTAG+TGG + chr2_4:57630251-57630270 None:intergenic 30.0%
TGGATGAAATAGCAAAAACA+TGG + chr2_4:57630310-57630329 None:intergenic 30.0%
ATCTGCTTCTTTATTGAAAG+AGG - chr2_4:57630401-57630420 Msa0337590:intron 30.0%
ATACTTAAAATTGGAGGAAG+AGG + chr2_4:57630499-57630518 None:intergenic 30.0%
CCATCAATACTTAAAATTGG+AGG + chr2_4:57630505-57630524 None:intergenic 30.0%
!!! CCTCCAATTTTAAGTATTGA+TGG - chr2_4:57630502-57630521 Msa0337590:intron 30.0%
AAACATTCAATCTAACGTGT+CGG + chr2_4:57630663-57630682 None:intergenic 30.0%
CAATATAACTCGCTTCATAT+CGG + chr2_4:57630850-57630869 None:intergenic 30.0%
ATAGACAATAAGTAGCTTCT+TGG - chr2_4:57630945-57630964 Msa0337590:intron 30.0%
AATACTAGATTTGTGATGCT+TGG - chr2_4:57631047-57631066 Msa0337590:intron 30.0%
TGTCATAAAGTTTATGTCTC+TGG - chr2_4:57631127-57631146 Msa0337590:intron 30.0%
TTGCAACATTAAACAAGAGA+AGG + chr2_4:57631253-57631272 None:intergenic 30.0%
TTAAACAAGAGAAGGACAAT+GGG + chr2_4:57631342-57631361 None:intergenic 30.0%
ATTAAACAAGAGAAGGACAA+TGG + chr2_4:57631343-57631362 None:intergenic 30.0%
! AACATCTAGAATTCGAGATA+AGG - chr2_4:57631548-57631567 Msa0337590:intron 30.0%
! ACATCTAGAATTCGAGATAA+GGG - chr2_4:57631549-57631568 Msa0337590:intron 30.0%
ATGCATGATATCTCTCATTA+TGG - chr2_4:57631588-57631607 Msa0337590:CDS 30.0%
ATGATGAAAATGATATCAGC+AGG + chr2_4:57631787-57631806 None:intergenic 30.0%
TAGATTACCATAATCTTGAC+AGG - chr2_4:57632171-57632190 Msa0337590:CDS 30.0%
ATTGGGAAATTTGGTTGTTA+TGG - chr2_4:57632199-57632218 Msa0337590:CDS 30.0%
GTTAGACAATGAGTTACATT+GGG - chr2_4:57632290-57632309 Msa0337590:five_prime_UTR 30.0%
ACCAACATTATTCAACTTCA+TGG - chr2_4:57632366-57632385 Msa0337590:five_prime_UTR 30.0%
ACCATGAAGTTGAATAATGT+TGG + chr2_4:57632370-57632389 None:intergenic 30.0%
CATTATTCAACTTCATGGTA+TGG - chr2_4:57632371-57632390 Msa0337590:five_prime_UTR 30.0%
TTATTCAACTTCATGGTATG+GGG - chr2_4:57632373-57632392 Msa0337590:five_prime_UTR 30.0%
! CTTTCTCTTCGACAGTTTTA+CGG + chr2_4:57628976-57628995 None:intergenic 35.0%
TTTCTTCAAGTCATCGTCTT+TGG + chr2_4:57629063-57629082 None:intergenic 35.0%
TATGATCAATATGGGGAAGA+TGG - chr2_4:57629344-57629363 Msa0337590:CDS 35.0%
ATGATCAATATGGGGAAGAT+GGG - chr2_4:57629345-57629364 Msa0337590:CDS 35.0%
TTCCGTATCAGTTCAATCAT+CGG - chr2_4:57629447-57629466 Msa0337590:CDS 35.0%
! CATAGCAACTTGTTCTCAAT+AGG + chr2_4:57629629-57629648 None:intergenic 35.0%
ACTGTGATCAAGAATTTCTC+TGG + chr2_4:57629699-57629718 None:intergenic 35.0%
AGAGAAATTCTTGATCACAG+TGG - chr2_4:57629698-57629717 Msa0337590:intron 35.0%
GAGAAATTCTTGATCACAGT+GGG - chr2_4:57629699-57629718 Msa0337590:intron 35.0%
CATTGAAAAAAGAACTAGGC+AGG + chr2_4:57629741-57629760 None:intergenic 35.0%
AAAGTAGCTCAGTTTCAGTT+TGG - chr2_4:57629799-57629818 Msa0337590:intron 35.0%
!! CTGATTAGTCTCTTATAGCT+CGG - chr2_4:57629939-57629958 Msa0337590:intron 35.0%
TAGAAAAGATCGTGGGAAAT+AGG - chr2_4:57630022-57630041 Msa0337590:intron 35.0%
ACCTGTACTGTAGATTATGT+AGG - chr2_4:57630074-57630093 Msa0337590:intron 35.0%
TCCTACATAATCTACAGTAC+AGG + chr2_4:57630078-57630097 None:intergenic 35.0%
GTACTGTAGATTATGTAGGA+AGG - chr2_4:57630078-57630097 Msa0337590:intron 35.0%
TTAAAATTGGAGGAAGAGGA+AGG + chr2_4:57630495-57630514 None:intergenic 35.0%
GTGTGACATGTATCACTATA+TGG - chr2_4:57630692-57630711 Msa0337590:intron 35.0%
TGTCTATGTCAATGCTTCAT+AGG - chr2_4:57630815-57630834 Msa0337590:intron 35.0%
CAATGCTTCATAGGTTAACA+TGG - chr2_4:57630824-57630843 Msa0337590:intron 35.0%
CGATATGAAGCGAGTTATAT+TGG - chr2_4:57630848-57630867 Msa0337590:intron 35.0%
TCCTATTTCATTACAGTCCA+GGG - chr2_4:57631168-57631187 Msa0337590:intron 35.0%
TGTTGCAAATTACAATGGCT+TGG - chr2_4:57631265-57631284 Msa0337590:intron 35.0%
!! ATGGCTTGGTGTTTGTTAAA+CGG - chr2_4:57631279-57631298 Msa0337590:intron 35.0%
TGTTAAACGGGATCAATAAC+TGG - chr2_4:57631292-57631311 Msa0337590:intron 35.0%
TAAACAAGAGAAGGACAATG+GGG + chr2_4:57631341-57631360 None:intergenic 35.0%
TGTTTAATGTTACAACGGCT+TGG - chr2_4:57631355-57631374 Msa0337590:intron 35.0%
TGTTAAACGGGATCAATAAC+TGG - chr2_4:57631292-57631311 Msa0337590:intron 35.0%
TCCCAAACAATATGCACAAT+GGG + chr2_4:57631432-57631451 None:intergenic 35.0%
ATCCCAAACAATATGCACAA+TGG + chr2_4:57631433-57631452 None:intergenic 35.0%
ATTGTGCATATTGTTTGGGA+TGG - chr2_4:57631432-57631451 Msa0337590:intron 35.0%
TTGACAATTAGTGTCAAACC+TGG - chr2_4:57631700-57631719 Msa0337590:CDS 35.0%
ATATCAGCAGGTATGGTATT+TGG + chr2_4:57631775-57631794 None:intergenic 35.0%
GAAAATGATATCAGCAGGTA+TGG + chr2_4:57631782-57631801 None:intergenic 35.0%
ATTCACTGTACAACCAACTA+AGG + chr2_4:57631887-57631906 None:intergenic 35.0%
ATTACCATAATCTTGACAGG+AGG - chr2_4:57632174-57632193 Msa0337590:CDS 35.0%
GGTTAGACAATGAGTTACAT+TGG - chr2_4:57632289-57632308 Msa0337590:five_prime_UTR 35.0%
! TTTTGCGGCGAATTTGTGAA+GGG + chr2_4:57628927-57628946 None:intergenic 40.0%
! ATTTTGCGGCGAATTTGTGA+AGG + chr2_4:57628928-57628947 None:intergenic 40.0%
! TCCGATTTTCTGCGATTTTG+CGG + chr2_4:57628942-57628961 None:intergenic 40.0%
TGTCGAAGAGAAAGAGAGAA+GGG - chr2_4:57628982-57629001 Msa0337590:three_prime_UTR 40.0%
TGACTACTACAAGCTTCTTC+AGG - chr2_4:57629025-57629044 Msa0337590:three_prime_UTR 40.0%
!! ACAAGCTTCTTCAGGTTGAT+CGG - chr2_4:57629033-57629052 Msa0337590:three_prime_UTR 40.0%
AACTGATACGGAAAGTCATC+AGG + chr2_4:57629440-57629459 None:intergenic 40.0%
GTCCGATGATTGAACTGATA+CGG + chr2_4:57629452-57629471 None:intergenic 40.0%
AATGGCATGTTTGGATCCTA+TGG - chr2_4:57629548-57629567 Msa0337590:CDS 40.0%
! AGCAACTTGTTCTCAATAGG+AGG + chr2_4:57629626-57629645 None:intergenic 40.0%
CAAGTTGCTATGTTCTCTTG+AGG - chr2_4:57629637-57629656 Msa0337590:intron 40.0%
GGTGATCTTCATCTTCTTAG+TGG + chr2_4:57629678-57629697 None:intergenic 40.0%
! ACAAACCCTCGTGGATTAAT+TGG - chr2_4:57629908-57629927 Msa0337590:intron 40.0%
CTTCTCCAATTAATCCACGA+GGG + chr2_4:57629916-57629935 None:intergenic 40.0%
TCTTCTCCAATTAATCCACG+AGG + chr2_4:57629917-57629936 None:intergenic 40.0%
ACCCATAGTAGAAAAGATCG+TGG - chr2_4:57630014-57630033 Msa0337590:intron 40.0%
! CCCACGATCTTTTCTACTAT+GGG + chr2_4:57630018-57630037 None:intergenic 40.0%
CCCATAGTAGAAAAGATCGT+GGG - chr2_4:57630015-57630034 Msa0337590:intron 40.0%
! TCCCACGATCTTTTCTACTA+TGG + chr2_4:57630019-57630038 None:intergenic 40.0%
GAAATAGGCTTAGGTGGTTT+AGG - chr2_4:57630037-57630056 Msa0337590:intron 40.0%
GAGGATAGTCAAATCACTAG+AGG - chr2_4:57630116-57630135 Msa0337590:intron 40.0%
ATTATCTCACTTAGTGGGGT+TGG + chr2_4:57630245-57630264 None:intergenic 40.0%
CCAGCATATTCTCTACAACA+TGG + chr2_4:57630330-57630349 None:intergenic 40.0%
CCATGTTGTAGAGAATATGC+TGG - chr2_4:57630327-57630346 Msa0337590:intron 40.0%
GTTGTAGAGAATATGCTGGT+AGG - chr2_4:57630331-57630350 Msa0337590:intron 40.0%
!! GTAGGTTTTGTAGTTGTGGT+TGG - chr2_4:57630349-57630368 Msa0337590:intron 40.0%
! CTGAGTTTTACACTCAAGTC+AGG - chr2_4:57630543-57630562 Msa0337590:intron 40.0%
!! TGAAGCATTGACATAGACAC+CGG + chr2_4:57630814-57630833 None:intergenic 40.0%
GTCCTATTTCATTACAGTCC+AGG - chr2_4:57631167-57631186 Msa0337590:intron 40.0%
TCCCTGGACTGTAATGAAAT+AGG + chr2_4:57631172-57631191 None:intergenic 40.0%
!! TGGCTTGGTGTTTGTTAAAC+GGG - chr2_4:57631280-57631299 Msa0337590:intron 40.0%
! GGGGTTAGTACATTAGACTA+AGG + chr2_4:57631322-57631341 None:intergenic 40.0%
!! ACGGCTTGGTGTTTGTTAAA+CGG - chr2_4:57631369-57631388 Msa0337590:intron 40.0%
! GGGGTTAGTACATTAGACTA+AGG + chr2_4:57631322-57631341 None:intergenic 40.0%
ACCCCATTGTGCATATTGTT+TGG - chr2_4:57631427-57631446 Msa0337590:intron 40.0%
CCCAAACAATATGCACAATG+GGG + chr2_4:57631431-57631450 None:intergenic 40.0%
CCCCATTGTGCATATTGTTT+GGG - chr2_4:57631428-57631447 Msa0337590:intron 40.0%
CAATTAGTGTCAAACCTGGA+TGG - chr2_4:57631704-57631723 Msa0337590:CDS 40.0%
TGGATGTTCATTTCCCTTCT+CGG + chr2_4:57631755-57631774 None:intergenic 40.0%
GTGAATCTCACTACACTTGA+TGG - chr2_4:57631901-57631920 Msa0337590:CDS 40.0%
TGTTGTTCATCCTGAGTATG+AGG - chr2_4:57631951-57631970 Msa0337590:CDS 40.0%
CCTTCTTTGTTGGATCCTTT+GGG + chr2_4:57632005-57632024 None:intergenic 40.0%
CCAAAGGATCCAACAAAGAA+GGG - chr2_4:57632003-57632022 Msa0337590:intron 40.0%
CTCAAGTTTCCCTTCTTTGT+TGG + chr2_4:57632015-57632034 None:intergenic 40.0%
CAACAAAGAAGGGAAACTTG+AGG - chr2_4:57632013-57632032 Msa0337590:intron 40.0%
TCAACGTCAAATTCCCAACT+CGG - chr2_4:57632043-57632062 Msa0337590:intron 40.0%
AGCAGGAATGAAGAAAGTCT+TGG - chr2_4:57632083-57632102 Msa0337590:intron 40.0%
ATTGCCTCCTGTCAAGATTA+TGG + chr2_4:57632181-57632200 None:intergenic 40.0%
TAATCTTGACAGGAGGCAAT+TGG - chr2_4:57632181-57632200 Msa0337590:CDS 40.0%
AATCTTGACAGGAGGCAATT+GGG - chr2_4:57632182-57632201 Msa0337590:CDS 40.0%
GCCGCAAAATCGCAGAAAAT+CGG - chr2_4:57628938-57628957 Msa0337590:three_prime_UTR 45.0%
! GACAGTTTTACGGCACTTTC+TGG + chr2_4:57628966-57628985 None:intergenic 45.0%
CTGTCGAAGAGAAAGAGAGA+AGG - chr2_4:57628981-57629000 Msa0337590:three_prime_UTR 45.0%
CTTGTAGTAGTCAACACCCA+TGG + chr2_4:57629018-57629037 None:intergenic 45.0%
ACAGAAAACTCGCCATGAAG+TGG - chr2_4:57629087-57629106 Msa0337590:three_prime_UTR 45.0%
! CTTATCAGGATGCCACTTCA+TGG + chr2_4:57629102-57629121 None:intergenic 45.0%
!!! TCTGGGTCATGCTGATTTTG+TGG - chr2_4:57629267-57629286 Msa0337590:CDS 45.0%
!!! GGTCATGCTGATTTTGTGGT+GGG - chr2_4:57629271-57629290 Msa0337590:CDS 45.0%
TTTGGGGATCGAAATCCCTT+TGG - chr2_4:57629497-57629516 Msa0337590:CDS 45.0%
TCGAAATCCCTTTGGTGGAA+TGG - chr2_4:57629505-57629524 Msa0337590:CDS 45.0%
CGAAATCCCTTTGGTGGAAT+GGG - chr2_4:57629506-57629525 Msa0337590:CDS 45.0%
AGAGGTAGCAATGGCATGTT+TGG - chr2_4:57629539-57629558 Msa0337590:CDS 45.0%
! TTTGGATCCTATGGTGATGC+TGG - chr2_4:57629557-57629576 Msa0337590:CDS 45.0%
AGTGGAGTCAATGTGCATCA+AGG - chr2_4:57629584-57629603 Msa0337590:CDS 45.0%
AACCAAAGTACAAACCCTCG+TGG - chr2_4:57629899-57629918 Msa0337590:intron 45.0%
ATCCACGAGGGTTTGTACTT+TGG + chr2_4:57629904-57629923 None:intergenic 45.0%
AGATCGTGGGAAATAGGCTT+AGG - chr2_4:57630028-57630047 Msa0337590:intron 45.0%
AGGAAGGACAGTAGATGACA+TGG - chr2_4:57630094-57630113 Msa0337590:intron 45.0%
AAGGACAGTAGATGACATGG+AGG - chr2_4:57630097-57630116 Msa0337590:intron 45.0%
AGTCAAATCACTAGAGGCAG+AGG - chr2_4:57630122-57630141 Msa0337590:intron 45.0%
!! GCTGGTAGGTTTTGTAGTTG+TGG - chr2_4:57630345-57630364 Msa0337590:intron 45.0%
! TGTGGTTGGTGCAATGTATG+TGG - chr2_4:57630363-57630382 Msa0337590:intron 45.0%
!! TGTATGTGGTGCCATTTGTG+TGG - chr2_4:57630377-57630396 Msa0337590:intron 45.0%
AGAAGCAGATGCCACACAAA+TGG + chr2_4:57630391-57630410 None:intergenic 45.0%
!! CTATATGGCATCGACACATG+TGG - chr2_4:57630707-57630726 Msa0337590:intron 45.0%
TGACACAGACACTTTGACAC+TGG + chr2_4:57630776-57630795 None:intergenic 45.0%
! CAAAGTGTCTGTGTCATGTC+TGG - chr2_4:57630780-57630799 Msa0337590:intron 45.0%
AGGGATGTAGAAGTCTTCTG+CGG - chr2_4:57631187-57631206 Msa0337590:intron 45.0%
!! CGGCTTGGTGTTTGTTAAAC+GGG - chr2_4:57631370-57631389 Msa0337590:intron 45.0%
TGTCAAACCTGGATGGAAGA+AGG - chr2_4:57631711-57631730 Msa0337590:CDS 45.0%
GTCAAACCTGGATGGAAGAA+GGG - chr2_4:57631712-57631731 Msa0337590:CDS 45.0%
TCAAACCTGGATGGAAGAAG+GGG - chr2_4:57631713-57631732 Msa0337590:CDS 45.0%
AAAGATCACTTTCCCCGAGA+AGG - chr2_4:57631738-57631757 Msa0337590:CDS 45.0%
AAGATCACTTTCCCCGAGAA+GGG - chr2_4:57631739-57631758 Msa0337590:CDS 45.0%
GATGTTCATTTCCCTTCTCG+GGG + chr2_4:57631753-57631772 None:intergenic 45.0%
GGATGTTCATTTCCCTTCTC+GGG + chr2_4:57631754-57631773 None:intergenic 45.0%
TCACGAGTGAACACATTGTG+TGG + chr2_4:57631820-57631839 None:intergenic 45.0%
CACAATGTGTTCACTCGTGA+AGG - chr2_4:57631820-57631839 Msa0337590:CDS 45.0%
TCTCTTGCAGAAGCCTTAGT+TGG - chr2_4:57631871-57631890 Msa0337590:CDS 45.0%
TGTTCATCCTGAGTATGAGG+AGG - chr2_4:57631954-57631973 Msa0337590:CDS 45.0%
! TTTGTTGGATCCTTTGGGAG+TGG + chr2_4:57632000-57632019 None:intergenic 45.0%
CCCAAAGGATCCAACAAAGA+AGG - chr2_4:57632002-57632021 Msa0337590:intron 45.0%
CCCTTCTTTGTTGGATCCTT+TGG + chr2_4:57632006-57632025 None:intergenic 45.0%
GGAATGAAGAAAGTCTTGGC+TGG - chr2_4:57632087-57632106 Msa0337590:intron 45.0%
CAGGAGGCAATTGGGAAATT+TGG - chr2_4:57632190-57632209 Msa0337590:CDS 45.0%
TAGTAGTCAACACCCATGGC+AGG + chr2_4:57629014-57629033 None:intergenic 50.0%
!!! GGGTCATGCTGATTTTGTGG+TGG - chr2_4:57629270-57629289 Msa0337590:CDS 50.0%
!! ATATGGGGAAGATGGGCTGA+AGG - chr2_4:57629352-57629371 Msa0337590:CDS 50.0%
!! TATGGGGAAGATGGGCTGAA+GGG - chr2_4:57629353-57629372 Msa0337590:CDS 50.0%
! CTACCAGAGGCATGAGAATC+AGG + chr2_4:57629395-57629414 None:intergenic 50.0%
GATTCTCATGCCTCTGGTAG+TGG - chr2_4:57629395-57629414 Msa0337590:CDS 50.0%
TCTACTACCACCACTACCAG+AGG + chr2_4:57629408-57629427 None:intergenic 50.0%
AATCCCTTTGGTGGAATGGG+AGG - chr2_4:57629509-57629528 Msa0337590:CDS 50.0%
ATCCCTTTGGTGGAATGGGA+GGG - chr2_4:57629510-57629529 Msa0337590:CDS 50.0%
ATCCCTCCCATTCCACCAAA+GGG + chr2_4:57629515-57629534 None:intergenic 50.0%
TTTGGTGGAATGGGAGGGAT+GGG - chr2_4:57629515-57629534 Msa0337590:CDS 50.0%
ACTTCCTCCAGCATCACCAT+AGG + chr2_4:57629567-57629586 None:intergenic 50.0%
! TATGGTGATGCTGGAGGAAG+TGG - chr2_4:57629566-57629585 Msa0337590:CDS 50.0%
TCGTGGGAAATAGGCTTAGG+TGG - chr2_4:57630031-57630050 Msa0337590:intron 50.0%
ACTAGAGGCAGAGGAAGACT+TGG - chr2_4:57630131-57630150 Msa0337590:intron 50.0%
!! ACTTAGTGGGGTTGGCTACA+TGG + chr2_4:57630237-57630256 None:intergenic 50.0%
GCAGAAGACTTCTACATCCC+TGG + chr2_4:57631188-57631207 None:intergenic 50.0%
GTGACTTTGCTGCACTCGAA+AGG + chr2_4:57631664-57631683 None:intergenic 50.0%
TTTGTCCCCTTCTTCCATCC+AGG + chr2_4:57631721-57631740 None:intergenic 50.0%
GGAACAACCTCCTCATACTC+AGG + chr2_4:57631964-57631983 None:intergenic 50.0%
GAGGAGGTTGTTCCAAGAGA+AGG - chr2_4:57631970-57631989 Msa0337590:CDS 50.0%
AGAAGGAATGCCACTCCCAA+AGG - chr2_4:57631987-57632006 Msa0337590:intron 50.0%
ATTCCCAACTCGGCTCACTT+CGG - chr2_4:57632053-57632072 Msa0337590:intron 50.0%
CTCACTTCGGATCAGAAAGC+AGG - chr2_4:57632066-57632085 Msa0337590:intron 50.0%
AGAAGGGCAACAACCTGCCA+TGG - chr2_4:57628998-57629017 Msa0337590:three_prime_UTR 55.0%
GAAGGGCAACAACCTGCCAT+GGG - chr2_4:57628999-57629018 Msa0337590:three_prime_UTR 55.0%
! GCATGAGAATCAGGTGGTGG+TGG + chr2_4:57629386-57629405 None:intergenic 55.0%
GAGGCATGAGAATCAGGTGG+TGG + chr2_4:57629389-57629408 None:intergenic 55.0%
! CCAGAGGCATGAGAATCAGG+TGG + chr2_4:57629392-57629411 None:intergenic 55.0%
CCACCTGATTCTCATGCCTC+TGG - chr2_4:57629389-57629408 Msa0337590:CDS 55.0%
!! TCTCATGCCTCTGGTAGTGG+TGG - chr2_4:57629398-57629417 Msa0337590:CDS 55.0%
GGGGATCGAAATCCCTTTGG+TGG - chr2_4:57629500-57629519 Msa0337590:CDS 55.0%
CATCCCTCCCATTCCACCAA+AGG + chr2_4:57629516-57629535 None:intergenic 55.0%
CTTTGGTGGAATGGGAGGGA+TGG - chr2_4:57629514-57629533 Msa0337590:CDS 55.0%
GGGATGGGAAGAGGTAGCAA+TGG - chr2_4:57629530-57629549 Msa0337590:CDS 55.0%
! GGATCCTATGGTGATGCTGG+AGG - chr2_4:57629560-57629579 Msa0337590:CDS 55.0%
GCATCAAGGTCCTCCTCGTA+AGG - chr2_4:57629598-57629617 Msa0337590:CDS 55.0%
!! TCAATAGGAGGCGCCTTACG+AGG + chr2_4:57629614-57629633 None:intergenic 55.0%
GGAAGAGGAAGGTTCGACTG+CGG + chr2_4:57630484-57630503 None:intergenic 55.0%
!! GTCATGTCTGGTGTCGTGTC+CGG - chr2_4:57630792-57630811 Msa0337590:intron 55.0%
GTGCAGCAAAGTCACATCGC+TGG - chr2_4:57631669-57631688 Msa0337590:CDS 55.0%
GGGAGTGGCATTCCTTCTCT+TGG + chr2_4:57631985-57632004 None:intergenic 55.0%
GATCCGAAGTGAGCCGAGTT+GGG + chr2_4:57632059-57632078 None:intergenic 55.0%
TGATCCGAAGTGAGCCGAGT+TGG + chr2_4:57632060-57632079 None:intergenic 55.0%
GGAATGGGAGGGATGGGAAG+AGG - chr2_4:57629521-57629540 Msa0337590:CDS 60.0%
!! ATAGGAGGCGCCTTACGAGG+AGG + chr2_4:57629611-57629630 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 57628861 57632442 57628861 ID=Msa0337590;Name=Msa0337590
chr2_4 mRNA 57628861 57632442 57628861 ID=Msa0337590-mRNA-1;Parent=Msa0337590;Name=Msa0337590-mRNA-1;_AED=0.06;_eAED=0.06;_QI=156|1|1|1|1|1|3|329|336
chr2_4 exon 57628861 57629628 57628861 ID=Msa0337590-mRNA-1:exon:17588;Parent=Msa0337590-mRNA-1
chr2_4 exon 57631583 57631989 57631583 ID=Msa0337590-mRNA-1:exon:17587;Parent=Msa0337590-mRNA-1
chr2_4 exon 57632122 57632442 57632122 ID=Msa0337590-mRNA-1:exon:17586;Parent=Msa0337590-mRNA-1
chr2_4 five_prime_UTR 57632287 57632442 57632287 ID=Msa0337590-mRNA-1:five_prime_utr;Parent=Msa0337590-mRNA-1
chr2_4 CDS 57632122 57632286 57632122 ID=Msa0337590-mRNA-1:cds;Parent=Msa0337590-mRNA-1
chr2_4 CDS 57631583 57631989 57631583 ID=Msa0337590-mRNA-1:cds;Parent=Msa0337590-mRNA-1
chr2_4 CDS 57629190 57629628 57629190 ID=Msa0337590-mRNA-1:cds;Parent=Msa0337590-mRNA-1
chr2_4 three_prime_UTR 57628861 57629189 57628861 ID=Msa0337590-mRNA-1:three_prime_utr;Parent=Msa0337590-mRNA-1
Gene Sequence

>Msa0337590

ATGGGTGTTGACTACTACAAGCTTCTTCAGGTTGATCGGAACGCCAAAGACGATGACTTGAAGAAAGCTTACAGAAAACTCGCCATGAAGTGGCATCCTGATAAGAACCCTAATAACAAAAAAGAAGCTGAAGCCAAATTTAAGCAAATCTCAGAAGCTTATGATGTATTGAGTGATTCTAACAAGAGAGCTGTTTATGATCAATATGGGGAAGATGGGCTGAAGGGTCAAATGCCACCACCACCTGATTCTCATGCCTCTGGTAGTGGTGGTAGTAGAAACTTTTCACCTGATGACTTTCCGTATCAGTTCAATCATCGGACTCCTGATGATATTTTTGCTGAGTTTTTTGGGGATCGAAATCCCTTTGGTGGAATGGGAGGGATGGGAAGAGGTAGCAATGGCATGTTTGGATCCTATGGTGATGCTGGAGGAAGTGGAGTCAATGTGCATCAAGGTCCTCCTCGTAAGGCGCCTCCTATTGAGAACAAGTTGCTATGTTCTCTTGAGGATATATATAAAGGGACCACTAAGAAGATGAAGATCACCAGAGAAATTCTTGATCACAGTGGCAAAGTCACATCGCTGGATGAAATCTTGACAATTAGTGTCAAACCTGGATGGAAGAAGGGGACAAAGATCACTTTCCCCGAGAAGGGAAATGAACATCCAAATACCATACCTGCTGATATCATTTTCATCATCGATGAAAAACCACACAATGTGTTCACTCGTGAAGGAAATGATCTAATTGTAACTCAGAAAATTTCTCTTGCAGAAGCCTTAGTTGGTTGTACAGTGAATCTCACTACACTTGATGGCAGAAATTTGACTGTAGTGATCAACAATGTTGTTCATCCTGAGTATGAGGAGGTTGTTCCAAGAGAAGGAATGCCACTCCCAAAGGATCCAACAAAGAAGGGAAACTTGAGGATAAAGTTCAACGTCAAATTCCCAACTCGGCTCACTTCGGATCAGAAAGCAGGAATGAAGAAAGTCTTGGCTGGATAA
Protein sequence

>Msa0337590

MGVDYYKLLQVDRNAKDDDLKKAYRKLAMKWHPDKNPNNKKEAEAKFKQISEAYDVLSDSNKRAVYDQYGEDGLKGQMPPPPDSHASGSGGSRNFSPDDFPYQFNHRTPDDIFAEFFGDRNPFGGMGGMGRGSNGMFGSYGDAGGSGVNVHQGPPRKAPPIENKLLCSLEDIYKGTTKKMKITREILDHSGKVTSLDEILTISVKPGWKKGTKITFPEKGNEHPNTIPADIIFIIDEKPHNVFTREGNDLIVTQKISLAEALVGCTVNLTTLDGRNLTVVINNVVHPEYEEVVPREGMPLPKDPTKKGNLRIKFNVKFPTRLTSDQKAGMKKVLAG*