AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1255110


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 36 sgRNAs with CRISPR-Local

Find 109 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAATATAGGTCCTATTTAT+AGG 0.244589 8_2:-53496227 None:intergenic
CCTATATGTGTCCTATAAAT+AGG 0.265061 8_2:+53496216 Msa1255110:three_prime_UTR
GCAACATTCTGCCACTTATC+TGG 0.266066 8_2:-53495468 None:intergenic
TGTAAATACATGGACATAAT+TGG 0.271100 8_2:-53496028 None:intergenic
AATCAGTTGAAGATGTTAAA+AGG 0.300561 8_2:+53495505 Msa1255110:CDS
AGCTCATCTTGTTCTTGAAA+TGG 0.307339 8_2:+53495377 None:intergenic
AACAATAGATAAATTAATAC+TGG 0.349088 8_2:-53496100 None:intergenic
ATAGGACACATATAGGTAAA+TGG 0.360529 8_2:-53496209 None:intergenic
TACAATCATATGGAAGGTTA+AGG 0.385469 8_2:-53496152 None:intergenic
GTGTCATTACATTGCAATAT+AGG 0.394320 8_2:-53496240 None:intergenic
CAAACAATTTGAAAGAGCTT+TGG 0.396764 8_2:+53495425 Msa1255110:CDS
TTTGTAAGTAAATTGCTTCA+AGG 0.401631 8_2:-53496060 None:intergenic
TGCGCCTAACATCTTCTTTG+AGG 0.404699 8_2:-53495539 None:intergenic
GCTCATCTTGTTCTTGAAAT+GGG 0.405032 8_2:+53495378 None:intergenic
GTTATGAATATACCTATTAG+TGG 0.422166 8_2:+53496120 Msa1255110:three_prime_UTR
AGGGATGGACATCCACTAAT+AGG 0.424190 8_2:-53496132 None:intergenic
CCTATTTATAGGACACATAT+AGG 0.451071 8_2:-53496216 None:intergenic
TAGTGGGAGTTACAATCATA+TGG 0.457289 8_2:-53496162 None:intergenic
TGGGATCAACTTGGACTCCA+AGG 0.513093 8_2:+53495397 Msa1255110:CDS
ACAATCATATGGAAGGTTAA+GGG 0.515996 8_2:-53496151 None:intergenic
TTGGTAGCTTGCAATGCAGT+TGG 0.521852 8_2:-53496009 None:intergenic
AGATCCTCAAAGAAGATGTT+AGG 0.522074 8_2:+53495535 Msa1255110:CDS
TTCTTGAAATGGGATCAACT+TGG 0.536988 8_2:+53495388 Msa1255110:CDS
ATAGAGATTCTCCAGATAAG+TGG 0.553229 8_2:+53495457 Msa1255110:CDS
CATTACAATCCACTCACTAG+TGG 0.573179 8_2:-53496179 None:intergenic
AACTGATTTCCCCACCATGT+TGG 0.577684 8_2:-53495490 None:intergenic
GATGTTAGGCGCATTGAGCA+TGG 0.583089 8_2:+53495549 Msa1255110:CDS
GGGAGTTACAATCATATGGA+AGG 0.611155 8_2:-53496158 None:intergenic
TCATATGGAAGGTTAAGGGA+TGG 0.617562 8_2:-53496147 None:intergenic
GTGGCAGAATGTTGCCAACA+TGG 0.629175 8_2:+53495476 Msa1255110:CDS
ATTACAATCCACTCACTAGT+GGG 0.631431 8_2:-53496178 None:intergenic
CAGAATGTTGCCAACATGGT+GGG 0.631728 8_2:+53495480 Msa1255110:CDS
TTGTAACTCCCACTAGTGAG+TGG 0.632254 8_2:+53496170 Msa1255110:three_prime_UTR
AGTAGTACTATCAACAGCAG+TGG 0.647471 8_2:+53495953 Msa1255110:three_prime_UTR
AGAATGTTGCCAACATGGTG+GGG 0.648486 8_2:+53495481 Msa1255110:CDS
GCAGAATGTTGCCAACATGG+TGG 0.688195 8_2:+53495479 Msa1255110:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTACTTATTAATTAACTA+TGG - chr8_2:53495924-53495943 None:intergenic 10.0%
!!! TTTTACTTATTAATTAACTA+TGG - chr8_2:53495924-53495943 None:intergenic 10.0%
!! GAAAAAAAAATGTAAATACA+TGG - chr8_2:53496041-53496060 None:intergenic 15.0%
!! AACAATAGATAAATTAATAC+TGG - chr8_2:53496103-53496122 None:intergenic 15.0%
!! GAAAAAAAAATGTAAATACA+TGG - chr8_2:53496041-53496060 None:intergenic 15.0%
!! AACAATAGATAAATTAATAC+TGG - chr8_2:53496103-53496122 None:intergenic 15.0%
!! TGATGTGAATAAAAAAATGT+GGG - chr8_2:53495738-53495757 None:intergenic 20.0%
!! ATGATGTGAATAAAAAAATG+TGG - chr8_2:53495739-53495758 None:intergenic 20.0%
!! TGATGTGAATAAAAAAATGT+GGG - chr8_2:53495738-53495757 None:intergenic 20.0%
!! ATGATGTGAATAAAAAAATG+TGG - chr8_2:53495739-53495758 None:intergenic 20.0%
! AATCAGTTGAAGATGTTAAA+AGG + chr8_2:53495505-53495524 Msa1255110:CDS 25.0%
! TAGCATTATTTGTTCTGTAA+GGG - chr8_2:53495587-53495606 None:intergenic 25.0%
! TTAGCATTATTTGTTCTGTA+AGG - chr8_2:53495588-53495607 None:intergenic 25.0%
! GGATGTGTATAAAAAAATGT+GGG - chr8_2:53495660-53495679 None:intergenic 25.0%
! TGTAAATACATGGACATAAT+TGG - chr8_2:53496031-53496050 None:intergenic 25.0%
! TTTGTAAGTAAATTGCTTCA+AGG - chr8_2:53496063-53496082 None:intergenic 25.0%
! GTTATGAATATACCTATTAG+TGG + chr8_2:53496120-53496139 Msa1255110:three_prime_UTR 25.0%
! AATCAGTTGAAGATGTTAAA+AGG + chr8_2:53495505-53495524 Msa1255110:CDS 25.0%
! TAGCATTATTTGTTCTGTAA+GGG - chr8_2:53495587-53495606 None:intergenic 25.0%
! TTAGCATTATTTGTTCTGTA+AGG - chr8_2:53495588-53495607 None:intergenic 25.0%
! GGATGTGTATAAAAAAATGT+GGG - chr8_2:53495660-53495679 None:intergenic 25.0%
! TGTAAATACATGGACATAAT+TGG - chr8_2:53496031-53496050 None:intergenic 25.0%
! TTTGTAAGTAAATTGCTTCA+AGG - chr8_2:53496063-53496082 None:intergenic 25.0%
! GTTATGAATATACCTATTAG+TGG + chr8_2:53496120-53496139 Msa1255110:three_prime_UTR 25.0%
!!! TCAAATTGTTTGTTTTGCCT+TGG - chr8_2:53495417-53495436 None:intergenic 30.0%
CAAACAATTTGAAAGAGCTT+TGG + chr8_2:53495425-53495444 Msa1255110:CDS 30.0%
!!! CCTTTTGTTATTAAAGACCA+AGG - chr8_2:53495620-53495639 None:intergenic 30.0%
! CCTTGGTCTTTAATAACAAA+AGG + chr8_2:53495617-53495636 Msa1255110:intron 30.0%
CGGATGTGTATAAAAAAATG+TGG - chr8_2:53495661-53495680 None:intergenic 30.0%
! TCTTCAATTTTGTTGGATTC+TGG - chr8_2:53495892-53495911 None:intergenic 30.0%
! ACTTGAGTCTTCAATTTTGT+TGG - chr8_2:53495899-53495918 None:intergenic 30.0%
ACAATCATATGGAAGGTTAA+GGG - chr8_2:53496154-53496173 None:intergenic 30.0%
TACAATCATATGGAAGGTTA+AGG - chr8_2:53496155-53496174 None:intergenic 30.0%
ATAGGACACATATAGGTAAA+TGG - chr8_2:53496212-53496231 None:intergenic 30.0%
CCTATTTATAGGACACATAT+AGG - chr8_2:53496219-53496238 None:intergenic 30.0%
CCTATATGTGTCCTATAAAT+AGG + chr8_2:53496216-53496235 Msa1255110:three_prime_UTR 30.0%
GCAATATAGGTCCTATTTAT+AGG - chr8_2:53496230-53496249 None:intergenic 30.0%
! GTGTCATTACATTGCAATAT+AGG - chr8_2:53496243-53496262 None:intergenic 30.0%
!!! TCAAATTGTTTGTTTTGCCT+TGG - chr8_2:53495417-53495436 None:intergenic 30.0%
CAAACAATTTGAAAGAGCTT+TGG + chr8_2:53495425-53495444 Msa1255110:CDS 30.0%
!!! CCTTTTGTTATTAAAGACCA+AGG - chr8_2:53495620-53495639 None:intergenic 30.0%
! CCTTGGTCTTTAATAACAAA+AGG + chr8_2:53495617-53495636 Msa1255110:intron 30.0%
CGGATGTGTATAAAAAAATG+TGG - chr8_2:53495661-53495680 None:intergenic 30.0%
! TCTTCAATTTTGTTGGATTC+TGG - chr8_2:53495892-53495911 None:intergenic 30.0%
! ACTTGAGTCTTCAATTTTGT+TGG - chr8_2:53495899-53495918 None:intergenic 30.0%
ACAATCATATGGAAGGTTAA+GGG - chr8_2:53496154-53496173 None:intergenic 30.0%
TACAATCATATGGAAGGTTA+AGG - chr8_2:53496155-53496174 None:intergenic 30.0%
ATAGGACACATATAGGTAAA+TGG - chr8_2:53496212-53496231 None:intergenic 30.0%
CCTATTTATAGGACACATAT+AGG - chr8_2:53496219-53496238 None:intergenic 30.0%
CCTATATGTGTCCTATAAAT+AGG + chr8_2:53496216-53496235 Msa1255110:three_prime_UTR 30.0%
GCAATATAGGTCCTATTTAT+AGG - chr8_2:53496230-53496249 None:intergenic 30.0%
! GTGTCATTACATTGCAATAT+AGG - chr8_2:53496243-53496262 None:intergenic 30.0%
TTCTTGAAATGGGATCAACT+TGG + chr8_2:53495388-53495407 Msa1255110:CDS 35.0%
ATAGAGATTCTCCAGATAAG+TGG + chr8_2:53495457-53495476 Msa1255110:CDS 35.0%
AGATCCTCAAAGAAGATGTT+AGG + chr8_2:53495535-53495554 Msa1255110:CDS 35.0%
AATGCTAATAACTGAGACCT+TGG + chr8_2:53495600-53495619 Msa1255110:intron 35.0%
AGATTCTGTACAAGATTGCA+CGG - chr8_2:53495681-53495700 None:intergenic 35.0%
TTTAGTATAGCTACAGCCTT+TGG + chr8_2:53495789-53495808 Msa1255110:intron 35.0%
AACATATATACAGCAGCCAA+AGG - chr8_2:53495808-53495827 None:intergenic 35.0%
TAATTCCTTCTTTCACTTGC+AGG + chr8_2:53495858-53495877 Msa1255110:intron 35.0%
!!! TTCTGTTCACTGCTGTTTTT+TGG - chr8_2:53495982-53496001 None:intergenic 35.0%
TAGTGGGAGTTACAATCATA+TGG - chr8_2:53496165-53496184 None:intergenic 35.0%
ATTACAATCCACTCACTAGT+GGG - chr8_2:53496181-53496200 None:intergenic 35.0%
TTCTTGAAATGGGATCAACT+TGG + chr8_2:53495388-53495407 Msa1255110:CDS 35.0%
ATAGAGATTCTCCAGATAAG+TGG + chr8_2:53495457-53495476 Msa1255110:CDS 35.0%
AGATCCTCAAAGAAGATGTT+AGG + chr8_2:53495535-53495554 Msa1255110:CDS 35.0%
AATGCTAATAACTGAGACCT+TGG + chr8_2:53495600-53495619 Msa1255110:intron 35.0%
AGATTCTGTACAAGATTGCA+CGG - chr8_2:53495681-53495700 None:intergenic 35.0%
TTTAGTATAGCTACAGCCTT+TGG + chr8_2:53495789-53495808 Msa1255110:intron 35.0%
AACATATATACAGCAGCCAA+AGG - chr8_2:53495808-53495827 None:intergenic 35.0%
TAATTCCTTCTTTCACTTGC+AGG + chr8_2:53495858-53495877 Msa1255110:intron 35.0%
!!! TTCTGTTCACTGCTGTTTTT+TGG - chr8_2:53495982-53496001 None:intergenic 35.0%
TAGTGGGAGTTACAATCATA+TGG - chr8_2:53496165-53496184 None:intergenic 35.0%
ATTACAATCCACTCACTAGT+GGG - chr8_2:53496181-53496200 None:intergenic 35.0%
AGTAGTACTATCAACAGCAG+TGG + chr8_2:53495953-53495972 Msa1255110:three_prime_UTR 40.0%
TCATATGGAAGGTTAAGGGA+TGG - chr8_2:53496150-53496169 None:intergenic 40.0%
GGGAGTTACAATCATATGGA+AGG - chr8_2:53496161-53496180 None:intergenic 40.0%
CATTACAATCCACTCACTAG+TGG - chr8_2:53496182-53496201 None:intergenic 40.0%
CCCATTTCAAGAACAAGATG+AGG - chr8_2:53495381-53495400 None:intergenic 40.0%
AGTAGTACTATCAACAGCAG+TGG + chr8_2:53495953-53495972 Msa1255110:three_prime_UTR 40.0%
TCATATGGAAGGTTAAGGGA+TGG - chr8_2:53496150-53496169 None:intergenic 40.0%
GGGAGTTACAATCATATGGA+AGG - chr8_2:53496161-53496180 None:intergenic 40.0%
CATTACAATCCACTCACTAG+TGG - chr8_2:53496182-53496201 None:intergenic 40.0%
! GCAACATTCTGCCACTTATC+TGG - chr8_2:53495471-53495490 None:intergenic 45.0%
CAGAATGTTGCCAACATGGT+GGG + chr8_2:53495480-53495499 Msa1255110:CDS 45.0%
AGAATGTTGCCAACATGGTG+GGG + chr8_2:53495481-53495500 Msa1255110:CDS 45.0%
! AACTGATTTCCCCACCATGT+TGG - chr8_2:53495493-53495512 None:intergenic 45.0%
TGCGCCTAACATCTTCTTTG+AGG - chr8_2:53495542-53495561 None:intergenic 45.0%
GAGAACCTGCAAGTGAAAGA+AGG - chr8_2:53495866-53495885 None:intergenic 45.0%
! TTGGTAGCTTGCAATGCAGT+TGG - chr8_2:53496012-53496031 None:intergenic 45.0%
AGGGATGGACATCCACTAAT+AGG - chr8_2:53496135-53496154 None:intergenic 45.0%
TTGTAACTCCCACTAGTGAG+TGG + chr8_2:53496170-53496189 Msa1255110:three_prime_UTR 45.0%
! GCAACATTCTGCCACTTATC+TGG - chr8_2:53495471-53495490 None:intergenic 45.0%
CAGAATGTTGCCAACATGGT+GGG + chr8_2:53495480-53495499 Msa1255110:CDS 45.0%
AGAATGTTGCCAACATGGTG+GGG + chr8_2:53495481-53495500 Msa1255110:CDS 45.0%
! AACTGATTTCCCCACCATGT+TGG - chr8_2:53495493-53495512 None:intergenic 45.0%
TGCGCCTAACATCTTCTTTG+AGG - chr8_2:53495542-53495561 None:intergenic 45.0%
GAGAACCTGCAAGTGAAAGA+AGG - chr8_2:53495866-53495885 None:intergenic 45.0%
! TTGGTAGCTTGCAATGCAGT+TGG - chr8_2:53496012-53496031 None:intergenic 45.0%
AGGGATGGACATCCACTAAT+AGG - chr8_2:53496135-53496154 None:intergenic 45.0%
TTGTAACTCCCACTAGTGAG+TGG + chr8_2:53496170-53496189 Msa1255110:three_prime_UTR 45.0%
TGGGATCAACTTGGACTCCA+AGG + chr8_2:53495397-53495416 Msa1255110:CDS 50.0%
GTGGCAGAATGTTGCCAACA+TGG + chr8_2:53495476-53495495 Msa1255110:CDS 50.0%
GCAGAATGTTGCCAACATGG+TGG + chr8_2:53495479-53495498 Msa1255110:CDS 50.0%
GATGTTAGGCGCATTGAGCA+TGG + chr8_2:53495549-53495568 Msa1255110:CDS 50.0%
TGGGATCAACTTGGACTCCA+AGG + chr8_2:53495397-53495416 Msa1255110:CDS 50.0%
GTGGCAGAATGTTGCCAACA+TGG + chr8_2:53495476-53495495 Msa1255110:CDS 50.0%
GCAGAATGTTGCCAACATGG+TGG + chr8_2:53495479-53495498 Msa1255110:CDS 50.0%
GATGTTAGGCGCATTGAGCA+TGG + chr8_2:53495549-53495568 Msa1255110:CDS 50.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 53495379 53496305 53495379 ID=Msa1255110;Name=Msa1255110
chr8_2 mRNA 53495379 53496305 53495379 ID=Msa1255110-mRNA-1;Parent=Msa1255110;Name=Msa1255110-mRNA-1;_AED=0.11;_eAED=0.39;_QI=0|0|0.5|1|0|0|2|371|83
chr8_2 exon 53495379 53495575 53495379 ID=Msa1255110-mRNA-1:exon:16579;Parent=Msa1255110-mRNA-1
chr8_2 exon 53495880 53496305 53495880 ID=Msa1255110-mRNA-1:exon:16580;Parent=Msa1255110-mRNA-1
chr8_2 CDS 53495379 53495575 53495379 ID=Msa1255110-mRNA-1:cds;Parent=Msa1255110-mRNA-1
chr8_2 CDS 53495880 53495934 53495880 ID=Msa1255110-mRNA-1:cds;Parent=Msa1255110-mRNA-1
chr8_2 three_prime_UTR 53495935 53496305 53495935 ID=Msa1255110-mRNA-1:three_prime_utr;Parent=Msa1255110-mRNA-1
Gene Sequence

>Msa1255110

CTCATCTTGTTCTTGAAATGGGATCAACTTGGACTCCAAGGCAAAACAAACAATTTGAAAGAGCTTTGGCTATATATGATAGAGATTCTCCAGATAAGTGGCAGAATGTTGCCAACATGGTGGGGAAATCAGTTGAAGATGTTAAAAGGCATTATGAGATCCTCAAAGAAGATGTTAGGCGCATTGAGCATGGTCAAGTTCTCAATCCAGAATCCAACAAAATTGAAGACTCAAGTATCCATAGTTAATTAA

Protein sequence

>Msa1255110

LILFLKWDQLGLQGKTNNLKELWLYMIEILQISGRMLPTWWGNQLKMLKGIMRSSKKMLGALSMVKFSIQNPTKLKTQVSIVN*