AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1295560


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 36 sgRNAs with CRISPR-Local

Find 109 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAATATAGGTCCTATTTAT+AGG 0.244589 8_3:-53698879 None:intergenic
CCTATATGTGTCCTATAAAT+AGG 0.265061 8_3:+53698868 Msa1295560:three_prime_UTR
GCAACATTCTGCCACTTATC+TGG 0.266066 8_3:-53698120 None:intergenic
TGTAAATACATGGACATAAT+TGG 0.271100 8_3:-53698680 None:intergenic
AATCAGTTGAAGATGTTAAA+AGG 0.300561 8_3:+53698157 Msa1295560:CDS
AGCTCATCTTGTTCTTGAAA+TGG 0.307339 8_3:+53698029 None:intergenic
AACAATAGATAAATTAATAC+TGG 0.349088 8_3:-53698752 None:intergenic
ATAGGACACATATAGGTAAA+TGG 0.360529 8_3:-53698861 None:intergenic
TACAATCATATGGAAGGTTA+AGG 0.385469 8_3:-53698804 None:intergenic
GTGTCATTACATTGCAATAT+AGG 0.394320 8_3:-53698892 None:intergenic
CAAACAATTTGAAAGAGCTT+TGG 0.396764 8_3:+53698077 Msa1295560:CDS
TTTGTAAGTAAATTGCTTCA+AGG 0.401631 8_3:-53698712 None:intergenic
TGCGCCTAACATCTTCTTTG+AGG 0.404699 8_3:-53698191 None:intergenic
GCTCATCTTGTTCTTGAAAT+GGG 0.405032 8_3:+53698030 None:intergenic
GTTATGAATATACCTATTAG+TGG 0.422166 8_3:+53698772 Msa1295560:three_prime_UTR
AGGGATGGACATCCACTAAT+AGG 0.424190 8_3:-53698784 None:intergenic
CCTATTTATAGGACACATAT+AGG 0.451071 8_3:-53698868 None:intergenic
TAGTGGGAGTTACAATCATA+TGG 0.457289 8_3:-53698814 None:intergenic
TGGGATCAACTTGGACTCCA+AGG 0.513093 8_3:+53698049 Msa1295560:CDS
ACAATCATATGGAAGGTTAA+GGG 0.515996 8_3:-53698803 None:intergenic
TTGGTAGCTTGCAATGCAGT+TGG 0.521852 8_3:-53698661 None:intergenic
AGATCCTCAAAGAAGATGTT+AGG 0.522074 8_3:+53698187 Msa1295560:CDS
TTCTTGAAATGGGATCAACT+TGG 0.536988 8_3:+53698040 Msa1295560:CDS
ATAGAGATTCTCCAGATAAG+TGG 0.553229 8_3:+53698109 Msa1295560:CDS
CATTACAATCCACTCACTAG+TGG 0.573179 8_3:-53698831 None:intergenic
AACTGATTTCCCCACCATGT+TGG 0.577684 8_3:-53698142 None:intergenic
GATGTTAGGCGCATTGAGCA+TGG 0.583089 8_3:+53698201 Msa1295560:CDS
GGGAGTTACAATCATATGGA+AGG 0.611155 8_3:-53698810 None:intergenic
TCATATGGAAGGTTAAGGGA+TGG 0.617562 8_3:-53698799 None:intergenic
GTGGCAGAATGTTGCCAACA+TGG 0.629175 8_3:+53698128 Msa1295560:CDS
ATTACAATCCACTCACTAGT+GGG 0.631431 8_3:-53698830 None:intergenic
CAGAATGTTGCCAACATGGT+GGG 0.631728 8_3:+53698132 Msa1295560:CDS
TTGTAACTCCCACTAGTGAG+TGG 0.632254 8_3:+53698822 Msa1295560:three_prime_UTR
AGTAGTACTATCAACAGCAG+TGG 0.647471 8_3:+53698605 Msa1295560:three_prime_UTR
AGAATGTTGCCAACATGGTG+GGG 0.648486 8_3:+53698133 Msa1295560:CDS
GCAGAATGTTGCCAACATGG+TGG 0.688195 8_3:+53698131 Msa1295560:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTACTTATTAATTAACTA+TGG - chr8_3:53698576-53698595 None:intergenic 10.0%
!!! TTTTACTTATTAATTAACTA+TGG - chr8_3:53698576-53698595 None:intergenic 10.0%
!! GAAAAAAAAATGTAAATACA+TGG - chr8_3:53698693-53698712 None:intergenic 15.0%
!! AACAATAGATAAATTAATAC+TGG - chr8_3:53698755-53698774 None:intergenic 15.0%
!! GAAAAAAAAATGTAAATACA+TGG - chr8_3:53698693-53698712 None:intergenic 15.0%
!! AACAATAGATAAATTAATAC+TGG - chr8_3:53698755-53698774 None:intergenic 15.0%
!! TGATGTGAATAAAAAAATGT+GGG - chr8_3:53698390-53698409 None:intergenic 20.0%
!! ATGATGTGAATAAAAAAATG+TGG - chr8_3:53698391-53698410 None:intergenic 20.0%
!! TGATGTGAATAAAAAAATGT+GGG - chr8_3:53698390-53698409 None:intergenic 20.0%
!! ATGATGTGAATAAAAAAATG+TGG - chr8_3:53698391-53698410 None:intergenic 20.0%
! AATCAGTTGAAGATGTTAAA+AGG + chr8_3:53698157-53698176 Msa1295560:CDS 25.0%
! TAGCATTATTTGTTCTGTAA+GGG - chr8_3:53698239-53698258 None:intergenic 25.0%
! TTAGCATTATTTGTTCTGTA+AGG - chr8_3:53698240-53698259 None:intergenic 25.0%
! GGATGTGTATAAAAAAATGT+GGG - chr8_3:53698312-53698331 None:intergenic 25.0%
! TGTAAATACATGGACATAAT+TGG - chr8_3:53698683-53698702 None:intergenic 25.0%
! TTTGTAAGTAAATTGCTTCA+AGG - chr8_3:53698715-53698734 None:intergenic 25.0%
! GTTATGAATATACCTATTAG+TGG + chr8_3:53698772-53698791 Msa1295560:three_prime_UTR 25.0%
! AATCAGTTGAAGATGTTAAA+AGG + chr8_3:53698157-53698176 Msa1295560:CDS 25.0%
! TAGCATTATTTGTTCTGTAA+GGG - chr8_3:53698239-53698258 None:intergenic 25.0%
! TTAGCATTATTTGTTCTGTA+AGG - chr8_3:53698240-53698259 None:intergenic 25.0%
! GGATGTGTATAAAAAAATGT+GGG - chr8_3:53698312-53698331 None:intergenic 25.0%
! TGTAAATACATGGACATAAT+TGG - chr8_3:53698683-53698702 None:intergenic 25.0%
! TTTGTAAGTAAATTGCTTCA+AGG - chr8_3:53698715-53698734 None:intergenic 25.0%
! GTTATGAATATACCTATTAG+TGG + chr8_3:53698772-53698791 Msa1295560:three_prime_UTR 25.0%
!!! TCAAATTGTTTGTTTTGCCT+TGG - chr8_3:53698069-53698088 None:intergenic 30.0%
CAAACAATTTGAAAGAGCTT+TGG + chr8_3:53698077-53698096 Msa1295560:CDS 30.0%
!!! CCTTTTGTTATTAAAGACCA+AGG - chr8_3:53698272-53698291 None:intergenic 30.0%
! CCTTGGTCTTTAATAACAAA+AGG + chr8_3:53698269-53698288 Msa1295560:intron 30.0%
CGGATGTGTATAAAAAAATG+TGG - chr8_3:53698313-53698332 None:intergenic 30.0%
! TCTTCAATTTTGTTGGATTC+TGG - chr8_3:53698544-53698563 None:intergenic 30.0%
! ACTTGAGTCTTCAATTTTGT+TGG - chr8_3:53698551-53698570 None:intergenic 30.0%
ACAATCATATGGAAGGTTAA+GGG - chr8_3:53698806-53698825 None:intergenic 30.0%
TACAATCATATGGAAGGTTA+AGG - chr8_3:53698807-53698826 None:intergenic 30.0%
ATAGGACACATATAGGTAAA+TGG - chr8_3:53698864-53698883 None:intergenic 30.0%
CCTATTTATAGGACACATAT+AGG - chr8_3:53698871-53698890 None:intergenic 30.0%
CCTATATGTGTCCTATAAAT+AGG + chr8_3:53698868-53698887 Msa1295560:three_prime_UTR 30.0%
GCAATATAGGTCCTATTTAT+AGG - chr8_3:53698882-53698901 None:intergenic 30.0%
! GTGTCATTACATTGCAATAT+AGG - chr8_3:53698895-53698914 None:intergenic 30.0%
!!! TCAAATTGTTTGTTTTGCCT+TGG - chr8_3:53698069-53698088 None:intergenic 30.0%
CAAACAATTTGAAAGAGCTT+TGG + chr8_3:53698077-53698096 Msa1295560:CDS 30.0%
!!! CCTTTTGTTATTAAAGACCA+AGG - chr8_3:53698272-53698291 None:intergenic 30.0%
! CCTTGGTCTTTAATAACAAA+AGG + chr8_3:53698269-53698288 Msa1295560:intron 30.0%
CGGATGTGTATAAAAAAATG+TGG - chr8_3:53698313-53698332 None:intergenic 30.0%
! TCTTCAATTTTGTTGGATTC+TGG - chr8_3:53698544-53698563 None:intergenic 30.0%
! ACTTGAGTCTTCAATTTTGT+TGG - chr8_3:53698551-53698570 None:intergenic 30.0%
ACAATCATATGGAAGGTTAA+GGG - chr8_3:53698806-53698825 None:intergenic 30.0%
TACAATCATATGGAAGGTTA+AGG - chr8_3:53698807-53698826 None:intergenic 30.0%
ATAGGACACATATAGGTAAA+TGG - chr8_3:53698864-53698883 None:intergenic 30.0%
CCTATTTATAGGACACATAT+AGG - chr8_3:53698871-53698890 None:intergenic 30.0%
CCTATATGTGTCCTATAAAT+AGG + chr8_3:53698868-53698887 Msa1295560:three_prime_UTR 30.0%
GCAATATAGGTCCTATTTAT+AGG - chr8_3:53698882-53698901 None:intergenic 30.0%
! GTGTCATTACATTGCAATAT+AGG - chr8_3:53698895-53698914 None:intergenic 30.0%
TTCTTGAAATGGGATCAACT+TGG + chr8_3:53698040-53698059 Msa1295560:CDS 35.0%
ATAGAGATTCTCCAGATAAG+TGG + chr8_3:53698109-53698128 Msa1295560:CDS 35.0%
AGATCCTCAAAGAAGATGTT+AGG + chr8_3:53698187-53698206 Msa1295560:CDS 35.0%
AATGCTAATAACTGAGACCT+TGG + chr8_3:53698252-53698271 Msa1295560:intron 35.0%
AGATTCTGTACAAGATTGCA+CGG - chr8_3:53698333-53698352 None:intergenic 35.0%
TTTAGTATAGCTACAGCCTT+TGG + chr8_3:53698441-53698460 Msa1295560:intron 35.0%
AACATATATACAGCAGCCAA+AGG - chr8_3:53698460-53698479 None:intergenic 35.0%
TAATTCCTTCTTTCACTTGC+AGG + chr8_3:53698510-53698529 Msa1295560:intron 35.0%
!!! TTCTGTTCACTGCTGTTTTT+TGG - chr8_3:53698634-53698653 None:intergenic 35.0%
TAGTGGGAGTTACAATCATA+TGG - chr8_3:53698817-53698836 None:intergenic 35.0%
ATTACAATCCACTCACTAGT+GGG - chr8_3:53698833-53698852 None:intergenic 35.0%
TTCTTGAAATGGGATCAACT+TGG + chr8_3:53698040-53698059 Msa1295560:CDS 35.0%
ATAGAGATTCTCCAGATAAG+TGG + chr8_3:53698109-53698128 Msa1295560:CDS 35.0%
AGATCCTCAAAGAAGATGTT+AGG + chr8_3:53698187-53698206 Msa1295560:CDS 35.0%
AATGCTAATAACTGAGACCT+TGG + chr8_3:53698252-53698271 Msa1295560:intron 35.0%
AGATTCTGTACAAGATTGCA+CGG - chr8_3:53698333-53698352 None:intergenic 35.0%
TTTAGTATAGCTACAGCCTT+TGG + chr8_3:53698441-53698460 Msa1295560:intron 35.0%
AACATATATACAGCAGCCAA+AGG - chr8_3:53698460-53698479 None:intergenic 35.0%
TAATTCCTTCTTTCACTTGC+AGG + chr8_3:53698510-53698529 Msa1295560:intron 35.0%
!!! TTCTGTTCACTGCTGTTTTT+TGG - chr8_3:53698634-53698653 None:intergenic 35.0%
TAGTGGGAGTTACAATCATA+TGG - chr8_3:53698817-53698836 None:intergenic 35.0%
ATTACAATCCACTCACTAGT+GGG - chr8_3:53698833-53698852 None:intergenic 35.0%
AGTAGTACTATCAACAGCAG+TGG + chr8_3:53698605-53698624 Msa1295560:three_prime_UTR 40.0%
TCATATGGAAGGTTAAGGGA+TGG - chr8_3:53698802-53698821 None:intergenic 40.0%
GGGAGTTACAATCATATGGA+AGG - chr8_3:53698813-53698832 None:intergenic 40.0%
CATTACAATCCACTCACTAG+TGG - chr8_3:53698834-53698853 None:intergenic 40.0%
CCCATTTCAAGAACAAGATG+AGG - chr8_3:53698033-53698052 None:intergenic 40.0%
AGTAGTACTATCAACAGCAG+TGG + chr8_3:53698605-53698624 Msa1295560:three_prime_UTR 40.0%
TCATATGGAAGGTTAAGGGA+TGG - chr8_3:53698802-53698821 None:intergenic 40.0%
GGGAGTTACAATCATATGGA+AGG - chr8_3:53698813-53698832 None:intergenic 40.0%
CATTACAATCCACTCACTAG+TGG - chr8_3:53698834-53698853 None:intergenic 40.0%
! GCAACATTCTGCCACTTATC+TGG - chr8_3:53698123-53698142 None:intergenic 45.0%
CAGAATGTTGCCAACATGGT+GGG + chr8_3:53698132-53698151 Msa1295560:CDS 45.0%
AGAATGTTGCCAACATGGTG+GGG + chr8_3:53698133-53698152 Msa1295560:CDS 45.0%
! AACTGATTTCCCCACCATGT+TGG - chr8_3:53698145-53698164 None:intergenic 45.0%
TGCGCCTAACATCTTCTTTG+AGG - chr8_3:53698194-53698213 None:intergenic 45.0%
GAGAACCTGCAAGTGAAAGA+AGG - chr8_3:53698518-53698537 None:intergenic 45.0%
! TTGGTAGCTTGCAATGCAGT+TGG - chr8_3:53698664-53698683 None:intergenic 45.0%
AGGGATGGACATCCACTAAT+AGG - chr8_3:53698787-53698806 None:intergenic 45.0%
TTGTAACTCCCACTAGTGAG+TGG + chr8_3:53698822-53698841 Msa1295560:three_prime_UTR 45.0%
! GCAACATTCTGCCACTTATC+TGG - chr8_3:53698123-53698142 None:intergenic 45.0%
CAGAATGTTGCCAACATGGT+GGG + chr8_3:53698132-53698151 Msa1295560:CDS 45.0%
AGAATGTTGCCAACATGGTG+GGG + chr8_3:53698133-53698152 Msa1295560:CDS 45.0%
! AACTGATTTCCCCACCATGT+TGG - chr8_3:53698145-53698164 None:intergenic 45.0%
TGCGCCTAACATCTTCTTTG+AGG - chr8_3:53698194-53698213 None:intergenic 45.0%
GAGAACCTGCAAGTGAAAGA+AGG - chr8_3:53698518-53698537 None:intergenic 45.0%
! TTGGTAGCTTGCAATGCAGT+TGG - chr8_3:53698664-53698683 None:intergenic 45.0%
AGGGATGGACATCCACTAAT+AGG - chr8_3:53698787-53698806 None:intergenic 45.0%
TTGTAACTCCCACTAGTGAG+TGG + chr8_3:53698822-53698841 Msa1295560:three_prime_UTR 45.0%
TGGGATCAACTTGGACTCCA+AGG + chr8_3:53698049-53698068 Msa1295560:CDS 50.0%
GTGGCAGAATGTTGCCAACA+TGG + chr8_3:53698128-53698147 Msa1295560:CDS 50.0%
GCAGAATGTTGCCAACATGG+TGG + chr8_3:53698131-53698150 Msa1295560:CDS 50.0%
GATGTTAGGCGCATTGAGCA+TGG + chr8_3:53698201-53698220 Msa1295560:CDS 50.0%
TGGGATCAACTTGGACTCCA+AGG + chr8_3:53698049-53698068 Msa1295560:CDS 50.0%
GTGGCAGAATGTTGCCAACA+TGG + chr8_3:53698128-53698147 Msa1295560:CDS 50.0%
GCAGAATGTTGCCAACATGG+TGG + chr8_3:53698131-53698150 Msa1295560:CDS 50.0%
GATGTTAGGCGCATTGAGCA+TGG + chr8_3:53698201-53698220 Msa1295560:CDS 50.0%
Chromosome Type Strat End Strand Name
chr8_3 gene 53698031 53698957 53698031 ID=Msa1295560;Name=Msa1295560
chr8_3 mRNA 53698031 53698957 53698031 ID=Msa1295560-mRNA-1;Parent=Msa1295560;Name=Msa1295560-mRNA-1;_AED=0.11;_eAED=0.39;_QI=0|0|0.5|1|0|0|2|371|83
chr8_3 exon 53698031 53698227 53698031 ID=Msa1295560-mRNA-1:exon:15453;Parent=Msa1295560-mRNA-1
chr8_3 exon 53698532 53698957 53698532 ID=Msa1295560-mRNA-1:exon:15454;Parent=Msa1295560-mRNA-1
chr8_3 CDS 53698031 53698227 53698031 ID=Msa1295560-mRNA-1:cds;Parent=Msa1295560-mRNA-1
chr8_3 CDS 53698532 53698586 53698532 ID=Msa1295560-mRNA-1:cds;Parent=Msa1295560-mRNA-1
chr8_3 three_prime_UTR 53698587 53698957 53698587 ID=Msa1295560-mRNA-1:three_prime_utr;Parent=Msa1295560-mRNA-1
Gene Sequence

>Msa1295560

CTCATCTTGTTCTTGAAATGGGATCAACTTGGACTCCAAGGCAAAACAAACAATTTGAAAGAGCTTTGGCTATATATGATAGAGATTCTCCAGATAAGTGGCAGAATGTTGCCAACATGGTGGGGAAATCAGTTGAAGATGTTAAAAGGCATTATGAGATCCTCAAAGAAGATGTTAGGCGCATTGAGCATGGTCAAGTTCTCAATCCAGAATCCAACAAAATTGAAGACTCAAGTATCCATAGTTAATTAA

Protein sequence

>Msa1295560

LILFLKWDQLGLQGKTNNLKELWLYMIEILQISGRMLPTWWGNQLKMLKGIMRSSKKMLGALSMVKFSIQNPTKLKTQVSIVN*