Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1440720 | sp|C0LGI2|Y1677_ARATH | 68.216 | 925 | 279 | 6 | 9 | 923 | 10 | 929 | 0.0 | 1242 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0025330 | Msa1440720 | 0.818016 | 2.442378e-52 | -8.615850e-47 |
| Msa0034110 | Msa1440720 | 0.810579 | 1.074627e-50 | -8.615850e-47 |
| Msa0036140 | Msa1440720 | 0.834864 | 2.342386e-56 | -8.615850e-47 |
| Msa0072900 | Msa1440720 | 0.810290 | 1.240723e-50 | -8.615850e-47 |
| Msa0080090 | Msa1440720 | 0.808978 | 2.374198e-50 | -8.615850e-47 |
| Msa0121090 | Msa1440720 | 0.812498 | 4.113745e-51 | -8.615850e-47 |
| Msa0128120 | Msa1440720 | 0.811240 | 7.728595e-51 | -8.615850e-47 |
| Msa0143560 | Msa1440720 | 0.810050 | 1.397588e-50 | -8.615850e-47 |
| Msa0160130 | Msa1440720 | 0.801693 | 7.982404e-49 | -8.615850e-47 |
| Msa0170140 | Msa1440720 | 0.806882 | 6.627336e-50 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1440720 | MtrunA17_Chr3g0123141 | 96.667 | 930 | 24 | 1 | 1 | 923 | 1 | 930 | 0.0 | 1871 |
| Msa1440720 | MtrunA17_Chr7g0263591 | 50.677 | 886 | 408 | 11 | 22 | 894 | 30 | 899 | 0.0 | 883 |
| Msa1440720 | MtrunA17_Chr1g0181731 | 50.512 | 879 | 406 | 12 | 20 | 885 | 22 | 884 | 0.0 | 857 |
| Msa1440720 | MtrunA17_Chr8g0341011 | 33.630 | 898 | 467 | 25 | 25 | 878 | 34 | 846 | 1.89e-140 | 440 |
| Msa1440720 | MtrunA17_Chr8g0340931 | 33.370 | 920 | 486 | 27 | 1 | 878 | 8 | 842 | 7.71e-140 | 438 |
| Msa1440720 | MtrunA17_Chr8g0340881 | 33.077 | 910 | 478 | 28 | 4 | 867 | 12 | 836 | 2.15e-139 | 437 |
| Msa1440720 | MtrunA17_Chr8g0341151 | 32.427 | 919 | 496 | 28 | 1 | 878 | 2 | 836 | 3.65e-139 | 436 |
| Msa1440720 | MtrunA17_Chr8g0340891 | 31.585 | 896 | 514 | 22 | 4 | 872 | 12 | 835 | 9.12e-133 | 419 |
| Msa1440720 | MtrunA17_Chr8g0340871 | 32.318 | 919 | 506 | 26 | 1 | 878 | 8 | 851 | 3.70e-131 | 416 |
| Msa1440720 | MtrunA17_Chr8g0340911 | 32.048 | 908 | 508 | 28 | 1 | 874 | 6 | 838 | 4.76e-130 | 412 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1440720 | AT1G67720.1 | 68.216 | 925 | 279 | 6 | 9 | 923 | 10 | 929 | 0.0 | 1242 |
| Msa1440720 | AT2G37050.1 | 48.866 | 882 | 419 | 14 | 15 | 879 | 18 | 884 | 0.0 | 824 |
| Msa1440720 | AT2G37050.3 | 48.753 | 882 | 421 | 15 | 15 | 879 | 18 | 885 | 0.0 | 823 |
| Msa1440720 | AT2G37050.2 | 46.835 | 711 | 355 | 11 | 15 | 717 | 18 | 713 | 0.0 | 634 |
| Msa1440720 | AT2G37050.4 | 46.695 | 711 | 357 | 12 | 15 | 717 | 18 | 714 | 0.0 | 634 |
| Msa1440720 | AT2G14510.1 | 33.876 | 921 | 504 | 25 | 4 | 893 | 7 | 853 | 1.55e-141 | 443 |
| Msa1440720 | AT2G14510.2 | 33.876 | 921 | 504 | 25 | 4 | 893 | 15 | 861 | 2.43e-141 | 443 |
| Msa1440720 | AT3G21340.1 | 33.081 | 925 | 531 | 23 | 2 | 891 | 12 | 883 | 1.37e-138 | 436 |
| Msa1440720 | AT1G51805.1 | 33.298 | 943 | 492 | 28 | 6 | 891 | 6 | 868 | 3.21e-138 | 435 |
| Msa1440720 | AT1G51800.1 | 32.841 | 947 | 513 | 31 | 1 | 893 | 1 | 878 | 3.09e-136 | 430 |
Find 206 sgRNAs with CRISPR-Local
Find 352 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAACCCACCCACCCACAAA+AGG | 0.440546 | tig0029109:-15624 | Msa1440720:intergenic |
| AAAATAAAGCCATTGAAGAT+AGG | 0.411440 | tig0029109:-10346 | Msa1440720:intergenic |
| AAAATCACTTCTTGCATTCT+TGG | 0.150730 | tig0029109:-15378 | Msa1440720:intergenic |
| AAAATTGGAAAGGGAAGCTT+TGG | 0.346086 | tig0029109:+13957 | Msa1440720:CDS |
| AAACATAGCAATCGAGACAA+GGG | 0.621323 | tig0029109:+11863 | Msa1440720:CDS |
| AAACTCACATTCATGTAACT+TGG | 0.482715 | tig0029109:-12128 | Msa1440720:intergenic |
| AAAGGCCTTATTAGGCAGGT+AGG | 0.633122 | tig0029109:-15606 | Msa1440720:intergenic |
| AAAGGGAACTGTGGGCTACC+TGG | 0.506502 | tig0029109:+14684 | Msa1440720:CDS |
| AAAGTAATAAAGTTAATAAG+TGG | 0.464436 | tig0029109:+10225 | Msa1440720:five_prime_UTR |
| AAATTGATGGTTCCCATGGC+TGG | 0.422405 | tig0029109:-14045 | Msa1440720:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATGCACATGATATTAACACA+AGG | - | tig0029109:10391-10410 | Msa1440720:intergenic | 30.0% | |
| TTATACACATACCTTGAAGT+TGG | - | tig0029109:10418-10437 | Msa1440720:intergenic | 30.0% | |
| TTTACTTGGATGCAACTAAA+TGG | + | tig0029109:10830-10849 | Msa1440720:CDS | 30.0% | |
| TTACTTGGATGCAACTAAAT+GGG | + | tig0029109:10831-10850 | Msa1440720:CDS | 30.0% | |
| CAAACATCAACAGAATTTGA+AGG | - | tig0029109:10916-10935 | Msa1440720:intergenic | 30.0% | |
| AGCTCAATAGTAGATATGAA+AGG | - | tig0029109:10964-10983 | Msa1440720:intergenic | 30.0% | |
| TCATATCTACTATTGAGCTT+AGG | + | tig0029109:10965-10984 | Msa1440720:CDS | 30.0% | |
| GCATACATAGAAAGATTCAA+GGG | - | tig0029109:10991-11010 | Msa1440720:intergenic | 30.0% | |
| TCATAGGTAATCTTGTAAAC+TGG | + | tig0029109:11294-11313 | Msa1440720:intron | 30.0% | |
| GTCTTTCTATTATCAATCCT+AGG | - | tig0029109:11355-11374 | Msa1440720:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0029109 | gene | 10199 | 15642 | 10199 | ID=Msa1440720;Name=Msa1440720 |
| tig0029109 | mRNA | 10199 | 15642 | 10199 | ID=Msa1440720-mRNA-1;Parent=Msa1440720;Name=Msa1440720-mRNA-1;_AED=0.14;_eAED=0.14;_QI=157|0.92|0.93|1|1|1|15|76|923 |
| tig0029109 | exon | 10199 | 10425 | 10199 | ID=Msa1440720-mRNA-1:exon:12405;Parent=Msa1440720-mRNA-1 |
| tig0029109 | exon | 10548 | 11088 | 10548 | ID=Msa1440720-mRNA-1:exon:12406;Parent=Msa1440720-mRNA-1 |
| tig0029109 | exon | 11758 | 12269 | 11758 | ID=Msa1440720-mRNA-1:exon:12407;Parent=Msa1440720-mRNA-1 |
| tig0029109 | exon | 12388 | 12517 | 12388 | ID=Msa1440720-mRNA-1:exon:12408;Parent=Msa1440720-mRNA-1 |
| tig0029109 | exon | 12832 | 12903 | 12832 | ID=Msa1440720-mRNA-1:exon:12409;Parent=Msa1440720-mRNA-1 |
| tig0029109 | exon | 12991 | 13059 | 12991 | ID=Msa1440720-mRNA-1:exon:12410;Parent=Msa1440720-mRNA-1 |
| tig0029109 | exon | 13155 | 13226 | 13155 | ID=Msa1440720-mRNA-1:exon:12411;Parent=Msa1440720-mRNA-1 |
| tig0029109 | exon | 13364 | 13432 | 13364 | ID=Msa1440720-mRNA-1:exon:12412;Parent=Msa1440720-mRNA-1 |
| tig0029109 | exon | 13575 | 13741 | 13575 | ID=Msa1440720-mRNA-1:exon:12413;Parent=Msa1440720-mRNA-1 |
| tig0029109 | exon | 13823 | 14076 | 13823 | ID=Msa1440720-mRNA-1:exon:12414;Parent=Msa1440720-mRNA-1 |
| tig0029109 | exon | 14153 | 14279 | 14153 | ID=Msa1440720-mRNA-1:exon:12415;Parent=Msa1440720-mRNA-1 |
| tig0029109 | exon | 14361 | 14426 | 14361 | ID=Msa1440720-mRNA-1:exon:12416;Parent=Msa1440720-mRNA-1 |
| tig0029109 | exon | 14548 | 14713 | 14548 | ID=Msa1440720-mRNA-1:exon:12417;Parent=Msa1440720-mRNA-1 |
| tig0029109 | exon | 14801 | 14933 | 14801 | ID=Msa1440720-mRNA-1:exon:12418;Parent=Msa1440720-mRNA-1 |
| tig0029109 | exon | 15243 | 15642 | 15243 | ID=Msa1440720-mRNA-1:exon:12419;Parent=Msa1440720-mRNA-1 |
| tig0029109 | five_prime_UTR | 10199 | 10355 | 10199 | ID=Msa1440720-mRNA-1:five_prime_utr;Parent=Msa1440720-mRNA-1 |
| tig0029109 | CDS | 10356 | 10425 | 10356 | ID=Msa1440720-mRNA-1:cds;Parent=Msa1440720-mRNA-1 |
| tig0029109 | CDS | 10548 | 11088 | 10548 | ID=Msa1440720-mRNA-1:cds;Parent=Msa1440720-mRNA-1 |
| tig0029109 | CDS | 11758 | 12269 | 11758 | ID=Msa1440720-mRNA-1:cds;Parent=Msa1440720-mRNA-1 |
| tig0029109 | CDS | 12388 | 12517 | 12388 | ID=Msa1440720-mRNA-1:cds;Parent=Msa1440720-mRNA-1 |
| tig0029109 | CDS | 12832 | 12903 | 12832 | ID=Msa1440720-mRNA-1:cds;Parent=Msa1440720-mRNA-1 |
| tig0029109 | CDS | 12991 | 13059 | 12991 | ID=Msa1440720-mRNA-1:cds;Parent=Msa1440720-mRNA-1 |
| tig0029109 | CDS | 13155 | 13226 | 13155 | ID=Msa1440720-mRNA-1:cds;Parent=Msa1440720-mRNA-1 |
| tig0029109 | CDS | 13364 | 13432 | 13364 | ID=Msa1440720-mRNA-1:cds;Parent=Msa1440720-mRNA-1 |
| tig0029109 | CDS | 13575 | 13741 | 13575 | ID=Msa1440720-mRNA-1:cds;Parent=Msa1440720-mRNA-1 |
| tig0029109 | CDS | 13823 | 14076 | 13823 | ID=Msa1440720-mRNA-1:cds;Parent=Msa1440720-mRNA-1 |
| tig0029109 | CDS | 14153 | 14279 | 14153 | ID=Msa1440720-mRNA-1:cds;Parent=Msa1440720-mRNA-1 |
| tig0029109 | CDS | 14361 | 14426 | 14361 | ID=Msa1440720-mRNA-1:cds;Parent=Msa1440720-mRNA-1 |
| tig0029109 | CDS | 14548 | 14713 | 14548 | ID=Msa1440720-mRNA-1:cds;Parent=Msa1440720-mRNA-1 |
| tig0029109 | CDS | 14801 | 14933 | 14801 | ID=Msa1440720-mRNA-1:cds;Parent=Msa1440720-mRNA-1 |
| tig0029109 | CDS | 15243 | 15566 | 15243 | ID=Msa1440720-mRNA-1:cds;Parent=Msa1440720-mRNA-1 |
| tig0029109 | three_prime_UTR | 15567 | 15642 | 15567 | ID=Msa1440720-mRNA-1:three_prime_utr;Parent=Msa1440720-mRNA-1 |
| Gene Sequence |
>Msa1440720 ATGGCTTTATTTTTTCTCTTTCTCTTAACACTCCTTGTGTTAATATCATGTGCATCTTGCCAACTTCAAGAATTTATAAGCATTGATTGTGGAGGAACAAGAAGTAACTACACTGACACAACCACAGGACTAACATGGATTTCAGATTCTGAGATTATGAAACATGGAGAGACAGTAGAAGTAAAAACCCCTAATGGAACCAAAGTTCAGTATCAGAAACGCAGAGATTTTCCAACAGATAGCAGAAAATATTGTTATACACTTGAGACTGAAGAGAGAAGAAGGTATTTGGTAAGAGCAACTTTTCAGTATGGAAGTTTGCAAAATGGAGACACATACCCTCAGTTTCAACTTTACTTGGATGCAACTAAATGGGCTACTGTGTCAATTTATGATGAATCAAGAATATATGTTAAGGAAATGATCTTTAGGGCACCTTCAAATTCTGTTGATGTTTGCATGTGTTGTGCTACAACTGGTTCTCCTTTCATATCTACTATTGAGCTTAGGCCCTTGAATCTTTCTATGTATGCCACTGATTATGAGGATGACTTCTTCTTGAAAGTAGCTGCAAGAATTAACTTTGGTGCTCCAACTGGGGATGCTGTCAGGTATCCAGAGGATCCATATGATAGAATATGGGAGTCTGATCTTGGAAAAAGACAAAATTTTCTTGTTGGGGTGGCCCCAGGAACCGAAAGAATAAACACAACAAGAAACATAGCAATCGAGACAAGGGAATATCCACCAGTTAAGGTGATGCAAACTGCGGTTGTTGGCACAAGAGGACTACTTAGCTATAGACTGAATCTTGAAGACTTTCCAGGGAACGCAAGAGCTTATGCATATTTAGCAGAGATTGAAGATCTGAGTCAGAATGAGACTAGAAAATTCAAATTGGAGCAACCTTTTATAGCTGATTATAGCAATGCAGTGGTGAACATAGCTGAGAATGCTAATGGGAGCTACACTCTTTATGAACCAAGTTACATGAATGTGAGTTTGGAATTTGTTCTTTCCTTCTCCTTTAAGAGGACGCCGGATTCTACTAGAGGACCTCTACTAAATGCAATGGAAATAAGCAAATATCAAGAAATTGCTTCAAAGACACTCAAGCAAGATTCAAATTATGTAAATGCATTTTCCTCCTTGTCTGATGAAATTATCCCAAAGAATGAAGGTGATCCTTGCGTTCCAACTCCATGGGAGTGGGTGAACTGTAGTACCGCTACACCTGCAAGAATTACAAAGATAAACTTGTCAGGAAGGAATTTGACCGGTGAAATCCCACGAGAGCTCAACAACATGGAAGCCTTGACAGAATTGTGGTTAGATAGAAACTTACTTACAGGACAACTACCAGACATGAGCAATCTTATCAATCTAAAGATTGTGCATCTAGAGAACAACAAATTGACCGGTCCACTACCAACATACCTGGGCAGTTTACCCGGTTTACAAGCATTGTACATACAGAATAACTCATTTACCGGGGATATACCAGCAGGATTATTATCTACAAAAATCACTTTCATCTATGATAATAATCCTGGACTGCATAAAAGGAGCAAAAAGCATTTTCCGTTGATAGTAGGAATTTCTATTGGAGTCCTAGTGATTCTATTGGTCATGTTCTTAGCGAGTTTAGTGTTATTGCGTTACCTTAGGAGAAAGGCTTCTCAACAGAAAAGTGATGAAAAAGCTATCTCAGGACGCAGTAGTACTAAACATTTAACTGGATACTCTTTTGGCCGGGATGGGAATTTAATGGACGAAGGTACTGCGTACTCTATTACTCTCTCAGACTTGAAAGTAGCTACAAACAATTTTTCAAAGAAAATTGGAAAGGGAAGCTTTGGATCTGTCTATTACGGGAAAATGAAAGATGGAAAAGAGATCGCAGTTAAGACTATGACTGATCCGTCCAGCCATGGGAACCATCAATTTGTAACTGAGGTAGCCCTCCTATCAAGAATTCATCACAGAAACTTGGTTCCTCTGATTGGATATTGTGAAGAAGAATATCAGCATATTCTGGTTTATGAGTATATGCACAATGGCACTCTAAGGGATCACATTCATGAATGTTTGGGTGAAAAGAAATTAGATTGGCTGACTCGTCTTCGAATTGCAGAAGATGCTGCGAAAGGATGCAATCCTAGTATCATTCACCGAGACGTAAAGACGAGCAACATTCTCCTAGATATCAATATGAGAGCAAAAGTGTCGGATTTTGGACTCTCGAGACTAGCCGAAGAAGATTTAACTCATATATCAAGTGTTGCAAAGGGAACTGTGGGCTACCTGGATCCTGAGTACTATGCAAATCAACAATTGACTGAAAAGAGTGATGTATACAGTTTTGGAGTTGTTCTATTGGAACTGATATGTGGAAAGAAGCCTGTATCACCAGAAGATTATGGGCCAGAAATGAATATAGTTCACTGGGCAAGGTCCTTAATTCGTAAAGGCGATATAATCAGCATTATGGATCCTTTACTAATTGGAAATGTGAAAACTGAGTCAATTTGGAGGGTTGCTGAAATAGCCATGCAATGTGTAGAACCACATGGTGCCTCTAGGCCAAGAATGCAAGAAGTGATTTTGGCTATACAAGATGCTTCTAAGATTGAAAAAGGAACAGAAAGTCAACTAAAAGTATCATCCTCAAGTAGTTCAAAACCACAATCTTCAAGGAAAACTTTACTTACAAGCTTTCTTGAAATTGAAAGCCCTGATTTGTCAAATGGTTGTCTTCCATCAGCAAGATAA |
| Protein sequence |
>Msa1440720 MALFFLFLLTLLVLISCASCQLQEFISIDCGGTRSNYTDTTTGLTWISDSEIMKHGETVEVKTPNGTKVQYQKRRDFPTDSRKYCYTLETEERRRYLVRATFQYGSLQNGDTYPQFQLYLDATKWATVSIYDESRIYVKEMIFRAPSNSVDVCMCCATTGSPFISTIELRPLNLSMYATDYEDDFFLKVAARINFGAPTGDAVRYPEDPYDRIWESDLGKRQNFLVGVAPGTERINTTRNIAIETREYPPVKVMQTAVVGTRGLLSYRLNLEDFPGNARAYAYLAEIEDLSQNETRKFKLEQPFIADYSNAVVNIAENANGSYTLYEPSYMNVSLEFVLSFSFKRTPDSTRGPLLNAMEISKYQEIASKTLKQDSNYVNAFSSLSDEIIPKNEGDPCVPTPWEWVNCSTATPARITKINLSGRNLTGEIPRELNNMEALTELWLDRNLLTGQLPDMSNLINLKIVHLENNKLTGPLPTYLGSLPGLQALYIQNNSFTGDIPAGLLSTKITFIYDNNPGLHKRSKKHFPLIVGISIGVLVILLVMFLASLVLLRYLRRKASQQKSDEKAISGRSSTKHLTGYSFGRDGNLMDEGTAYSITLSDLKVATNNFSKKIGKGSFGSVYYGKMKDGKEIAVKTMTDPSSHGNHQFVTEVALLSRIHHRNLVPLIGYCEEEYQHILVYEYMHNGTLRDHIHECLGEKKLDWLTRLRIAEDAAKGCNPSIIHRDVKTSNILLDINMRAKVSDFGLSRLAEEDLTHISSVAKGTVGYLDPEYYANQQLTEKSDVYSFGVVLLELICGKKPVSPEDYGPEMNIVHWARSLIRKGDIISIMDPLLIGNVKTESIWRVAEIAMQCVEPHGASRPRMQEVILAIQDASKIEKGTESQLKVSSSSSSKPQSSRKTLLTSFLEIESPDLSNGCLPSAR* |
