Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0017510 | Msa0036960 | 0.804224 | 2.394222e-49 | -8.615850e-47 |
| Msa0036960 | Msa0095540 | 0.832296 | 1.024911e-55 | -8.615850e-47 |
| Msa0036960 | Msa0141100 | 0.844522 | 7.178458e-59 | -8.615850e-47 |
| Msa0036960 | Msa0157290 | 0.803894 | 2.804105e-49 | -8.615850e-47 |
| Msa0036960 | Msa0183750 | 0.816900 | 4.356318e-52 | -8.615850e-47 |
| Msa0036960 | Msa0220300 | 0.819890 | 9.158961e-53 | -8.615850e-47 |
| Msa0036960 | Msa0225600 | 0.813440 | 2.557177e-51 | -8.615850e-47 |
| Msa0036960 | Msa0266450 | 0.820256 | 7.549556e-53 | -8.615850e-47 |
| Msa0036960 | Msa0374850 | 0.839278 | 1.743660e-57 | -8.615850e-47 |
| Msa0036960 | Msa0375090 | 0.824514 | 7.744077e-54 | -8.615850e-47 |
| Msa0036960 | Msa0470340 | 0.824600 | 7.393165e-54 | -8.615850e-47 |
| Msa0036960 | Msa0480950 | 0.809852 | 1.541724e-50 | -8.615850e-47 |
| Msa0036960 | Msa0531430 | 0.847993 | 8.132671e-60 | -8.615850e-47 |
| Msa0036960 | Msa0534590 | 0.835450 | 1.665879e-56 | -8.615850e-47 |
| Msa0036960 | Msa0590800 | 0.824240 | 8.983957e-54 | -8.615850e-47 |
| Msa0036960 | Msa0609430 | 0.800651 | 1.304224e-48 | -8.615850e-47 |
| Msa0036960 | Msa0611380 | 0.834617 | 2.702453e-56 | -8.615850e-47 |
| Msa0036960 | Msa0628640 | 0.826343 | 2.857453e-54 | -8.615850e-47 |
| Msa0036960 | Msa0631120 | 0.840305 | 9.422588e-58 | -8.615850e-47 |
| Msa0036960 | Msa0666030 | 0.809576 | 1.767289e-50 | -8.615850e-47 |
| Msa0036960 | Msa0669340 | 0.819376 | 1.199806e-52 | -8.615850e-47 |
| Msa0036960 | Msa0684500 | 0.806146 | 9.478007e-50 | -8.615850e-47 |
| Msa0036960 | Msa0692060 | 0.823311 | 1.483136e-53 | -8.615850e-47 |
| Msa0036960 | Msa0716450 | 0.814544 | 1.459406e-51 | -8.615850e-47 |
| Msa0036960 | Msa0717260 | 0.815670 | 8.207416e-52 | -8.615850e-47 |
| Msa0036960 | Msa0758320 | 0.817743 | 2.815294e-52 | -8.615850e-47 |
| Msa0036960 | Msa0837980 | 0.803009 | 4.276310e-49 | -8.615850e-47 |
| Msa0036960 | Msa0853320 | 0.803399 | 3.551941e-49 | -8.615850e-47 |
| Msa0036960 | Msa0880140 | 0.803050 | 4.194302e-49 | -8.615850e-47 |
| Msa0036960 | Msa0975340 | 0.804521 | 2.075822e-49 | -8.615850e-47 |
| Msa0036960 | Msa1013400 | 0.804708 | 1.897833e-49 | -8.615850e-47 |
| Msa0036960 | Msa1179460 | 0.810344 | 1.207878e-50 | -8.615850e-47 |
| Msa0036960 | Msa1203000 | 0.828307 | 9.666547e-55 | -8.615850e-47 |
| Msa0036960 | Msa1205960 | 0.809447 | 1.883797e-50 | -8.615850e-47 |
| Msa0036960 | Msa1219260 | 0.824489 | 7.849869e-54 | -8.615850e-47 |
| Msa0036960 | Msa1254700 | 0.834608 | 2.716561e-56 | -8.615850e-47 |
| Msa0036960 | Msa1255280 | 0.809548 | 1.791860e-50 | -8.615850e-47 |
| Msa0036960 | Msa1266960 | 0.815313 | 9.857140e-52 | -8.615850e-47 |
| Msa0036960 | Msa1397250 | 0.832775 | 7.796673e-56 | -8.615850e-47 |
| Msa0036960 | Msa1399560 | 0.816789 | 4.613259e-52 | -8.615850e-47 |
| Msa0036960 | Msa1401150 | 0.805937 | 1.048998e-49 | -8.615850e-47 |
| Msa0036960 | Msa1437190 | 0.813929 | 1.995470e-51 | -8.615850e-47 |
| Msa0036960 | Msa1443840 | 0.830873 | 2.298074e-55 | -8.615850e-47 |
| Msa0036960 | Msa1449730 | 0.841955 | 3.470978e-58 | -8.615850e-47 |
| Msa0036960 | Msa1460380 | 0.821595 | 3.713638e-53 | -8.615850e-47 |
| Msa0036960 | Msa1460390 | 0.808320 | 3.281575e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0036960 | MtrunA17_Chr1g0199761 | 94.444 | 36 | 2 | 0 | 1 | 36 | 1 | 36 | 1.24e-17 | 69.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 12 sgRNAs with CRISPR-Local
Find 35 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTACTTTGGGACTGAATTTC+TGG | 0.082262 | 1_1:-65816326 | None:intergenic |
| CAGTTTCACGTTATAAGACT+TGG | 0.387807 | 1_1:+65816269 | Msa0036960:CDS |
| ATGAAAATTCACATAATGAT+TGG | 0.393884 | 1_1:-65816756 | None:intergenic |
| AGAAGATTATGCTTCTCAAC+GGG | 0.498617 | 1_1:+65816677 | Msa0036960:CDS |
| AAAATGATATTGATGAGTGT+GGG | 0.536325 | 1_1:+65816233 | None:intergenic |
| ATTGATGAGTGTGGGGCACA+TGG | 0.591913 | 1_1:+65816241 | Msa0036960:CDS |
| AAGACTTGGAGTAAAGATAA+CGG | 0.603133 | 1_1:+65816283 | Msa0036960:CDS |
| TAGAAGATTATGCTTCTCAA+CGG | 0.625011 | 1_1:+65816676 | Msa0036960:CDS |
| TGGAACATCGAGGTGGCTGA+AGG | 0.628575 | 1_1:-65816736 | None:intergenic |
| AAATGATATTGATGAGTGTG+GGG | 0.676724 | 1_1:+65816234 | None:intergenic |
| TAATGATTGGAACATCGAGG+TGG | 0.726775 | 1_1:-65816743 | None:intergenic |
| ACATAATGATTGGAACATCG+AGG | 0.733437 | 1_1:-65816746 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAATTTCTAGTAAATCGAAA+TGG | - | chr1_1:65816443-65816462 | None:intergenic | 20.0% |
| !! | TTTGATGTGAATAATAATGT+GGG | - | chr1_1:65816557-65816576 | None:intergenic | 20.0% |
| !! | ATTTGATGTGAATAATAATG+TGG | - | chr1_1:65816558-65816577 | None:intergenic | 20.0% |
| ! | AAAACAGAAATGAAATCACT+TGG | - | chr1_1:65816510-65816529 | None:intergenic | 25.0% |
| AAGACTTGGAGTAAAGATAA+CGG | + | chr1_1:65816283-65816302 | Msa0036960:CDS | 30.0% | |
| !!! | TTGCTTTTTCTTTCCATCTT+TGG | + | chr1_1:65816354-65816373 | Msa0036960:intron | 30.0% |
| TGGATAAGTTAGTACTTAGT+AGG | - | chr1_1:65816490-65816509 | None:intergenic | 30.0% | |
| TGGGAATGAAATGCTTTATA+TGG | - | chr1_1:65816538-65816557 | None:intergenic | 30.0% | |
| ! | GCTAAAATTTTCAGACAACA+TGG | + | chr1_1:65816581-65816600 | Msa0036960:intron | 30.0% |
| TAGAAGATTATGCTTCTCAA+CGG | + | chr1_1:65816676-65816695 | Msa0036960:CDS | 30.0% | |
| CAGTTTCACGTTATAAGACT+TGG | + | chr1_1:65816269-65816288 | Msa0036960:CDS | 35.0% | |
| AAAAGCAATGCTGGTACTTT+GGG | - | chr1_1:65816342-65816361 | None:intergenic | 35.0% | |
| AAAAAGCAATGCTGGTACTT+TGG | - | chr1_1:65816343-65816362 | None:intergenic | 35.0% | |
| ATGGAAAGAAAAAGCAATGC+TGG | - | chr1_1:65816351-65816370 | None:intergenic | 35.0% | |
| AAGGAAGAAAAAGCCAAAGA+TGG | - | chr1_1:65816370-65816389 | None:intergenic | 35.0% | |
| ! | TTCCTTTTTGACCAGTTTCT+GGG | + | chr1_1:65816384-65816403 | Msa0036960:intron | 35.0% |
| AGTTAGTACTTAGTAGGTAC+TGG | - | chr1_1:65816484-65816503 | None:intergenic | 35.0% | |
| ! | AACAAGAAGTACGACGTTAT+TGG | - | chr1_1:65816609-65816628 | None:intergenic | 35.0% |
| AGAAGATTATGCTTCTCAAC+GGG | + | chr1_1:65816677-65816696 | Msa0036960:CDS | 35.0% | |
| ACATAATGATTGGAACATCG+AGG | - | chr1_1:65816749-65816768 | None:intergenic | 35.0% | |
| !! | GTACTTTGGGACTGAATTTC+TGG | - | chr1_1:65816329-65816348 | None:intergenic | 40.0% |
| ! | CTTCCTTTTTGACCAGTTTC+TGG | + | chr1_1:65816383-65816402 | Msa0036960:intron | 40.0% |
| ACTGGACAAGAGGACAATAA+AGG | - | chr1_1:65816466-65816485 | None:intergenic | 40.0% | |
| ! | CTCAGTCTTCTACGTTTTCT+GGG | + | chr1_1:65816638-65816657 | Msa0036960:intron | 40.0% |
| TAATGATTGGAACATCGAGG+TGG | - | chr1_1:65816746-65816765 | None:intergenic | 40.0% | |
| CACCCAGAAACTGGTCAAAA+AGG | - | chr1_1:65816389-65816408 | None:intergenic | 45.0% | |
| TTTCTGGGTGTCTTGGCTAA+GGG | + | chr1_1:65816399-65816418 | Msa0036960:intron | 45.0% | |
| !! | CTTAGTAGGTACTGGACAAG+AGG | - | chr1_1:65816476-65816495 | None:intergenic | 45.0% |
| CCAGAAAACGTAGAAGACTG+AGG | - | chr1_1:65816640-65816659 | None:intergenic | 45.0% | |
| ! | CCTCAGTCTTCTACGTTTTC+TGG | + | chr1_1:65816637-65816656 | Msa0036960:intron | 45.0% |
| ATTGATGAGTGTGGGGCACA+TGG | + | chr1_1:65816241-65816260 | Msa0036960:CDS | 50.0% | |
| ! | TGACCAGTTTCTGGGTGTCT+TGG | + | chr1_1:65816392-65816411 | Msa0036960:intron | 50.0% |
| TAGCCAAGACACCCAGAAAC+TGG | - | chr1_1:65816398-65816417 | None:intergenic | 50.0% | |
| ! | GTTTCTGGGTGTCTTGGCTA+AGG | + | chr1_1:65816398-65816417 | Msa0036960:intron | 50.0% |
| TGGAACATCGAGGTGGCTGA+AGG | - | chr1_1:65816739-65816758 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1_1 | gene | 65816236 | 65816770 | 65816236 | ID=Msa0036960;Name=Msa0036960 |
| chr1_1 | mRNA | 65816236 | 65816770 | 65816236 | ID=Msa0036960-mRNA-1;Parent=Msa0036960;Name=Msa0036960-mRNA-1;_AED=0.07;_eAED=0.08;_QI=0|0|0|1|0|0|2|0|78 |
| chr1_1 | exon | 65816236 | 65816344 | 65816236 | ID=Msa0036960-mRNA-1:exon:21231;Parent=Msa0036960-mRNA-1 |
| chr1_1 | exon | 65816643 | 65816770 | 65816643 | ID=Msa0036960-mRNA-1:exon:21232;Parent=Msa0036960-mRNA-1 |
| chr1_1 | CDS | 65816236 | 65816344 | 65816236 | ID=Msa0036960-mRNA-1:cds;Parent=Msa0036960-mRNA-1 |
| chr1_1 | CDS | 65816643 | 65816770 | 65816643 | ID=Msa0036960-mRNA-1:cds;Parent=Msa0036960-mRNA-1 |
| Gene Sequence |
| Protein sequence |
