AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene045581

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene045581.t1 MTR_4g078630 98.596 285 4 0 1 285 1 285 0.0 578
MS.gene045581.t1 MTR_7g071680 46.786 280 132 6 15 279 25 302 1.01e-77 238
MS.gene045581.t1 MTR_4g052340 47.955 269 126 4 17 282 12 269 1.03e-74 229
MS.gene045581.t1 MTR_4g052350 46.886 273 127 4 15 282 10 269 1.10e-71 222
MS.gene045581.t1 MTR_4g052300 46.768 263 122 4 18 275 7 256 2.32e-70 218
MS.gene045581.t1 MTR_7g107670 45.946 259 133 5 17 272 31 285 3.32e-70 219
MS.gene045581.t1 MTR_4g052320 45.977 261 128 3 18 275 7 257 9.36e-69 214
MS.gene045581.t1 MTR_7g083870 45.588 272 137 7 17 284 33 297 2.07e-67 212
MS.gene045581.t1 MTR_7g110130 44.776 268 136 4 14 277 1 260 3.68e-66 207
MS.gene045581.t1 MTR_3g020670 45.091 275 144 4 5 274 1 273 8.24e-65 204
MS.gene045581.t1 MTR_6g023590 45.136 257 130 4 18 270 12 261 1.06e-64 204
MS.gene045581.t1 MTR_6g023990 45.076 264 134 4 11 270 6 262 7.27e-64 202
MS.gene045581.t1 MTR_6g023610 45.490 255 128 4 18 268 12 259 1.51e-63 201
MS.gene045581.t1 MTR_7g110160 46.241 266 110 5 14 274 1 238 1.64e-62 197
MS.gene045581.t1 MTR_2g090165 42.435 271 143 5 14 280 1 262 2.95e-62 197
MS.gene045581.t1 MTR_6g023910 42.857 266 141 3 18 279 12 270 6.57e-62 197
MS.gene045581.t1 MTR_7g105460 45.018 271 135 5 18 283 12 273 1.89e-61 196
MS.gene045581.t1 MTR_6g023600 44.922 256 130 4 18 269 12 260 4.61e-61 194
MS.gene045581.t1 MTR_7g110140 45.525 257 129 4 18 271 5 253 5.25e-61 194
MS.gene045581.t1 MTR_6g023950 46.484 256 126 5 18 269 12 260 1.66e-60 193
MS.gene045581.t1 MTR_6g023600 44.141 256 126 5 18 269 12 254 3.35e-57 184
MS.gene045581.t1 MTR_7g105580 40.613 261 126 6 17 274 6 240 5.26e-56 181
MS.gene045581.t1 MTR_7g105590 48.168 191 95 2 17 204 6 195 8.15e-55 176
MS.gene045581.t1 MTR_6g035395 41.538 260 134 5 17 276 3 244 2.75e-53 174
MS.gene045581.t1 MTR_3g113870 42.714 199 108 4 32 227 34 229 2.59e-47 158
MS.gene045581.t1 MTR_7g105570 44.554 202 101 4 84 283 38 230 8.12e-39 136
MS.gene045581.t1 MTR_4g084800 49.242 132 64 1 17 145 11 142 8.28e-36 125
MS.gene045581.t1 MTR_7g113650 29.811 265 158 7 18 269 39 288 5.39e-24 99.0
MS.gene045581.t1 MTR_1g082760 30.189 265 157 6 18 269 39 288 7.86e-24 98.6
MS.gene045581.t1 MTR_4g132850 30.682 264 159 7 16 272 4 250 1.17e-23 97.1
MS.gene045581.t1 MTR_3g085740 30.000 270 163 9 10 270 66 318 4.27e-21 91.3
MS.gene045581.t1 MTR_3g068155 28.958 259 165 8 19 271 19 264 9.00e-21 89.7
MS.gene045581.t1 MTR_7g094510 30.385 260 160 6 19 269 6 253 9.16e-21 89.4
MS.gene045581.t1 MTR_3g068070 29.167 264 158 9 19 271 15 260 1.41e-20 89.0
MS.gene045581.t1 MTR_4g131740 27.113 284 177 8 1 270 23 290 4.88e-20 88.2
MS.gene045581.t1 MTR_3g068065 30.000 260 163 9 19 272 17 263 8.93e-20 87.0
MS.gene045581.t1 MTR_3g068085 30.943 265 152 12 19 271 15 260 3.08e-19 85.5
MS.gene045581.t1 MTR_1196s0010 30.800 250 157 5 22 269 277 512 3.60e-19 87.4
MS.gene045581.t1 MTR_7g094520 29.008 262 163 7 19 270 11 259 4.05e-18 82.0
MS.gene045581.t1 MTR_7g063360 29.008 262 165 7 19 271 16 265 1.15e-17 80.9
MS.gene045581.t1 MTR_4g064887 28.044 271 152 11 19 271 15 260 9.62e-17 78.6
MS.gene045581.t1 MTR_1g051165 27.208 283 170 7 10 271 4 271 1.91e-16 77.8
MS.gene045581.t1 MTR_4g058810 28.676 272 173 7 11 271 2 263 3.46e-15 74.3
MS.gene045581.t1 MTR_4g058840 28.897 263 167 6 19 271 11 263 4.25e-15 73.9
MS.gene045581.t1 MTR_4g058820 28.897 263 167 6 19 271 11 263 4.25e-15 73.9
MS.gene045581.t1 MTR_7g063390 27.757 263 167 7 19 271 16 265 1.69e-14 72.0
MS.gene045581.t1 MTR_7g063260 27.547 265 167 5 19 272 16 266 3.38e-14 71.2
MS.gene045581.t1 MTR_8g096580 29.592 196 129 3 22 211 48 240 5.95e-14 71.2
MS.gene045581.t1 MTR_3g068175 27.206 272 179 8 6 271 4 262 1.73e-13 69.3
MS.gene045581.t1 MTR_7g063320 26.336 262 170 6 19 270 15 263 2.12e-13 68.9
MS.gene045581.t1 MTR_8g442750 29.021 286 162 11 5 270 1 265 2.47e-13 69.3
MS.gene045581.t1 MTR_4g086400 28.922 204 127 4 21 208 6 207 9.22e-13 68.6
MS.gene045581.t1 MTR_4g097510 31.771 192 115 6 22 205 5 188 1.11e-12 67.0
MS.gene045581.t1 MTR_1g068955 28.358 268 168 7 12 268 7 261 1.87e-12 66.6
MS.gene045581.t1 MTR_8g096620 27.638 199 128 5 16 205 42 233 3.51e-12 66.2
MS.gene045581.t1 MTR_7g063330 26.616 263 171 7 19 272 15 264 5.10e-12 65.1
MS.gene045581.t1 MTR_3g068175 26.449 276 180 9 6 271 4 266 9.45e-12 64.3
MS.gene045581.t1 MTR_7g063270 26.792 265 168 7 19 272 16 265 1.36e-11 63.9
MS.gene045581.t1 MTR_8g044240 26.531 196 129 4 25 207 75 268 3.47e-11 63.2
MS.gene045581.t1 MTR_7g076660 30.000 160 97 6 22 166 8 167 3.49e-11 62.8
MS.gene045581.t1 MTR_7g080410 28.774 212 126 8 22 212 41 248 4.01e-11 62.8
MS.gene045581.t1 MTR_4g086410 28.000 200 128 3 21 205 6 204 4.47e-11 63.5
MS.gene045581.t1 MTR_7g080410 29.245 212 125 8 22 212 41 248 4.79e-11 62.4
MS.gene045581.t1 MTR_7g076660 30.435 161 95 7 22 166 7 166 7.11e-11 62.0
MS.gene045581.t1 MTR_7g076655 31.447 159 92 7 24 166 9 166 9.71e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene045581.t1 AT3G42960 58.889 270 108 3 15 282 4 272 1.59e-106 310
MS.gene045581.t1 AT3G51680 46.520 273 135 3 14 275 27 299 2.16e-72 225
MS.gene045581.t1 AT3G26770 45.977 261 131 7 17 272 39 294 2.86e-67 212
MS.gene045581.t1 AT2G47140 45.946 259 129 4 14 269 1 251 1.27e-66 208
MS.gene045581.t1 AT3G26760 42.803 264 145 5 17 276 34 295 2.13e-63 202
MS.gene045581.t1 AT4G03140 42.966 263 143 5 17 276 76 334 6.60e-63 202
MS.gene045581.t1 AT2G47120 41.634 257 137 5 18 271 6 252 3.87e-58 187
MS.gene045581.t1 AT2G47120 41.634 257 137 5 18 271 5 251 4.19e-58 187
MS.gene045581.t1 AT3G29250 42.692 260 132 6 18 271 5 253 1.57e-57 185
MS.gene045581.t1 AT3G29250 41.573 267 139 6 11 271 34 289 4.16e-57 185
MS.gene045581.t1 AT3G29260 42.045 264 135 6 14 271 1 252 4.80e-57 184
MS.gene045581.t1 AT2G47130 40.467 257 141 3 18 271 5 252 2.93e-56 182
MS.gene045581.t1 AT3G29250 42.471 259 132 6 19 271 44 291 4.40e-56 183
MS.gene045581.t1 AT1G52340 42.264 265 144 3 17 274 16 278 1.55e-55 181
MS.gene045581.t1 AT2G47150 28.800 250 120 5 21 270 3 194 2.27e-23 95.1
MS.gene045581.t1 AT2G29310 27.857 280 159 8 19 282 7 259 4.32e-22 93.2
MS.gene045581.t1 AT1G54870 28.679 265 161 6 18 269 81 330 9.00e-22 93.6
MS.gene045581.t1 AT1G54870 28.679 265 161 6 18 269 83 332 9.99e-22 93.6
MS.gene045581.t1 AT3G03980 30.037 273 157 8 16 269 11 268 2.32e-21 91.3
MS.gene045581.t1 AT4G05530 30.827 266 156 9 16 272 6 252 1.03e-20 89.4
MS.gene045581.t1 AT5G06060 28.958 259 165 7 19 271 9 254 1.72e-20 89.0
MS.gene045581.t1 AT5G18210 30.943 265 153 8 19 268 8 257 2.28e-20 88.6
MS.gene045581.t1 AT3G04000 28.676 272 163 7 16 269 11 269 2.45e-20 88.6
MS.gene045581.t1 AT5G18210 32.271 251 140 8 19 254 8 243 4.13e-20 88.2
MS.gene045581.t1 AT3G05260 29.930 284 167 10 1 270 20 285 6.62e-20 87.8
MS.gene045581.t1 AT2G30670 27.170 265 162 7 19 271 7 252 1.14e-19 86.7
MS.gene045581.t1 AT2G29330 27.899 276 164 8 19 282 7 259 4.73e-19 84.7
MS.gene045581.t1 AT1G24360 29.304 273 161 9 5 270 70 317 1.04e-18 84.7
MS.gene045581.t1 AT2G29320 27.437 277 165 8 19 282 13 266 8.16e-18 81.6
MS.gene045581.t1 AT2G29360 25.993 277 179 9 5 271 1 261 1.51e-17 80.9
MS.gene045581.t1 AT2G29290 28.261 276 163 9 19 282 7 259 1.58e-17 80.5
MS.gene045581.t1 AT2G29340 26.786 280 162 8 19 282 7 259 2.37e-17 80.1
MS.gene045581.t1 AT1G07450 27.863 262 163 9 19 271 8 252 2.65e-17 80.1
MS.gene045581.t1 AT2G29350 26.996 263 160 8 21 271 17 259 3.11e-17 80.1
MS.gene045581.t1 AT2G29150 29.151 271 160 9 11 269 8 258 3.35e-17 79.7
MS.gene045581.t1 AT2G29260 28.571 266 157 8 19 271 68 313 3.56e-17 80.5
MS.gene045581.t1 AT2G29340 26.786 280 162 8 19 282 7 259 4.51e-17 80.1
MS.gene045581.t1 AT1G63380 28.676 272 172 8 12 271 14 275 6.93e-17 79.3
MS.gene045581.t1 AT1G63380 28.676 272 172 8 12 271 7 268 7.29e-17 79.0
MS.gene045581.t1 AT1G63380 28.623 276 176 7 7 271 5 270 8.17e-17 79.0
MS.gene045581.t1 AT1G63380 28.676 272 172 8 12 271 11 272 8.22e-17 79.0
MS.gene045581.t1 AT2G29300 25.843 267 164 8 19 271 7 253 9.63e-17 78.6
MS.gene045581.t1 AT2G29310 27.039 233 138 7 55 282 52 257 1.07e-16 78.2
MS.gene045581.t1 AT1G62610 28.159 277 178 6 6 271 1 267 1.11e-16 78.6
MS.gene045581.t1 AT2G29300 25.843 267 164 8 19 271 7 253 1.49e-16 78.2
MS.gene045581.t1 AT1G62610 27.305 282 180 7 1 271 1 268 1.68e-16 78.2
MS.gene045581.t1 AT2G29370 27.437 277 175 10 5 271 1 261 1.69e-16 77.8
MS.gene045581.t1 AT1G62610 28.309 272 173 7 12 271 5 266 1.74e-16 77.8
MS.gene045581.t1 AT1G07440 27.957 279 159 10 19 282 12 263 1.82e-16 77.8
MS.gene045581.t1 AT3G55290 30.303 264 162 8 19 271 18 270 1.92e-16 77.8
MS.gene045581.t1 AT1G62610 28.309 272 173 7 12 271 11 272 1.99e-16 77.8
MS.gene045581.t1 AT3G55290 29.545 264 164 7 19 271 17 269 2.14e-16 77.8
MS.gene045581.t1 AT3G55310 29.699 266 161 8 19 271 17 269 2.34e-16 77.8
MS.gene045581.t1 AT4G13180 28.520 277 171 9 5 270 1 261 4.36e-16 76.6
MS.gene045581.t1 AT2G29310 29.648 199 119 5 19 205 7 196 5.88e-16 75.1
MS.gene045581.t1 AT2G29290 27.948 229 141 7 55 282 37 242 1.71e-14 72.0
MS.gene045581.t1 AT1G49670 28.502 207 118 4 21 205 6 204 2.37e-14 73.6
MS.gene045581.t1 AT1G49670 28.502 207 118 4 21 205 6 204 2.89e-14 73.2
MS.gene045581.t1 AT5G50770 31.553 206 124 6 7 205 38 233 4.52e-14 71.6
MS.gene045581.t1 AT2G29350 28.934 197 119 6 21 205 17 204 5.42e-14 70.1
MS.gene045581.t1 AT2G29350 26.368 201 133 5 72 271 35 221 1.10e-13 69.3
MS.gene045581.t1 AT3G46170 26.937 271 177 7 12 271 18 278 4.45e-13 68.6
MS.gene045581.t1 AT5G50600 28.571 245 149 9 4 232 31 265 4.56e-13 68.9
MS.gene045581.t1 AT5G50700 28.571 245 149 9 4 232 31 265 4.56e-13 68.9
MS.gene045581.t1 AT2G17845 28.195 266 164 9 19 269 47 300 8.27e-13 67.8
MS.gene045581.t1 AT2G29320 27.230 213 136 6 72 282 53 248 9.99e-13 67.0
MS.gene045581.t1 AT2G29320 28.788 198 119 6 19 203 13 201 3.90e-12 64.7
MS.gene045581.t1 AT3G47350 29.358 218 131 7 21 225 46 253 6.22e-12 65.5
MS.gene045581.t1 AT3G47350 28.634 227 139 7 12 225 37 253 7.92e-12 65.1
MS.gene045581.t1 AT2G29340 27.979 193 118 5 19 199 7 190 8.86e-12 63.5
MS.gene045581.t1 AT2G29340 27.979 193 118 5 19 199 7 190 8.86e-12 63.5

Find 44 sgRNAs with CRISPR-Local

Find 65 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAAAATTATTTGCAGAAAA+TGG 0.160943 4.1:-27318484 MS.gene045581:CDS
TGATTCAATGTCAGATTCTT+TGG 0.249438 4.1:+27318372 None:intergenic
CTCAAAATTTAGCTCCAAAA+AGG 0.265907 4.1:-27318643 MS.gene045581:intron
ATGAATGCAAGTTTGCTTAA+AGG 0.265952 4.1:-27317785 MS.gene045581:CDS
GAACAATTGAGTGAGTTTAT+TGG 0.274099 4.1:-27317809 MS.gene045581:CDS
TTACTTTCAATCAATCTCTT+TGG 0.274120 4.1:-27318106 MS.gene045581:CDS
GCTCATAATCTTTCAGTTGA+TGG 0.326713 4.1:-27317689 MS.gene045581:CDS
TGAATGCAAGTTTGCTTAAA+GGG 0.337129 4.1:-27317784 MS.gene045581:CDS
TTTCTCTTCACTTAGGATCT+TGG 0.355190 4.1:+27317627 None:intergenic
AGAAGTGCGGCGTGTGAATT+AGG 0.357629 4.1:-27317932 MS.gene045581:CDS
TTCAACAATGCAGGAATTGC+AGG 0.375946 4.1:-27318304 MS.gene045581:intron
TTGCAGCATGTTTGATTCCA+TGG 0.396161 4.1:+27318077 None:intergenic
CAGTTAACCTTTCAATATCA+TGG 0.401447 4.1:-27318348 MS.gene045581:CDS
ACAGGTGGTGCAAGAGGAAT+TGG 0.404629 4.1:-27318517 MS.gene045581:CDS
TGTTGAACATTATGTCCAAT+TGG 0.431580 4.1:+27318320 None:intergenic
GGTAAGGTAGCAGTTATAAC+AGG 0.432290 4.1:-27318535 MS.gene045581:CDS
AATATCATGGAAAGGCCAAT+TGG 0.441411 4.1:-27318335 MS.gene045581:CDS
TCAAGTGCAGCTGCAACTAT+AGG 0.447304 4.1:-27318010 MS.gene045581:CDS
CCCGTAATTGCAGGCTATGA+AGG 0.465657 4.1:-27318169 MS.gene045581:intron
GACATAATGTTCAACAATGC+AGG 0.481153 4.1:-27318313 MS.gene045581:CDS
AGGGTTGCTGAATCAATTGA+TGG 0.484224 4.1:-27318418 MS.gene045581:CDS
AATCTCTTTGGAACAATCCA+TGG 0.489840 4.1:-27318094 MS.gene045581:CDS
TACAATGTCAAAATCAGCTA+TGG 0.496795 4.1:-27317966 MS.gene045581:CDS
GGCCTTTCCATGATATTGAA+AGG 0.509972 4.1:+27318341 None:intergenic
TTCTAGATGAAGAAGGCACC+AGG 0.520405 4.1:-27318438 MS.gene045581:CDS
AACCTTTCAATATCATGGAA+AGG 0.544623 4.1:-27318343 MS.gene045581:CDS
GCAAGTTTGCTTAAAGGGAG+AGG 0.554131 4.1:-27317779 MS.gene045581:CDS
GTTAATTGTGTTTCTCCGCA+CGG 0.557216 4.1:-27317893 MS.gene045581:CDS
CATAATCTTTCAGTTGATGG+TGG 0.564721 4.1:-27317686 MS.gene045581:CDS
ATGTCAAAATCAGCTATGGA+TGG 0.568969 4.1:-27317962 MS.gene045581:CDS
ATCAATTGATTCAGCAACCC+TGG 0.571405 4.1:+27318420 None:intergenic
AAGCATAACAACACTTGACA+TGG 0.580277 4.1:-27318143 MS.gene045581:CDS
GCTGATGTTCTAGATGAAGA+AGG 0.589049 4.1:-27318445 MS.gene045581:CDS
GTTATAACAGGTGGTGCAAG+AGG 0.590120 4.1:-27318523 MS.gene045581:CDS
AGTGCAGCTGCAACTATAGG+TGG 0.592641 4.1:-27318007 MS.gene045581:CDS
AACAACTGATGATGTTGCAC+AGG 0.603862 4.1:-27317753 MS.gene045581:CDS
GCATTCAAAAGCATCTCAGA+AGG 0.609801 4.1:+27317866 None:intergenic
ATAGGTGGATTCGCTTCACA+TGG 0.610255 4.1:-27317992 MS.gene045581:CDS
GCTGCAAAAGCAATGATCAA+AGG 0.629252 4.1:-27318061 MS.gene045581:CDS
AAGGTAGCAGTTATAACAGG+TGG 0.652683 4.1:-27318532 MS.gene045581:CDS
ATCTCAGAAGGAACGCCGTG+CGG 0.661162 4.1:+27317878 None:intergenic
GGATGGATTAATGAGAAGTG+CGG 0.667924 4.1:-27317945 MS.gene045581:CDS
TCTAGATGAAGAAGGCACCA+GGG 0.716790 4.1:-27318437 MS.gene045581:CDS
TCTACATTCTGAATCATACG+TGG 0.728605 4.1:+27318668 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GAAATTTAATATCATATGAT+AGG + chr4.1:27317723-27317742 None:intergenic 15.0%
!!! AATTTTGGAAGAAAAAAAAA+TGG - chr4.1:27318053-27318072 MS.gene045581:CDS 15.0%
!!! TTTCTTTTGAAATGTTTTTA+AGG - chr4.1:27317744-27317763 MS.gene045581:CDS 15.0%
!!! ATAGTTTTGTTTCGATATTT+AGG - chr4.1:27318087-27318106 MS.gene045581:CDS 20.0%
!!! GAATGGTTTATTTTGAATTT+TGG - chr4.1:27318038-27318057 MS.gene045581:CDS 20.0%
!!! TTGAATGCTTTTAAATGTTA+CGG - chr4.1:27318457-27318476 MS.gene045581:CDS 20.0%
! GCAAAATTATTTGCAGAAAA+TGG - chr4.1:27317824-27317843 MS.gene045581:CDS 25.0%
! TTACTTTCAATCAATCTCTT+TGG - chr4.1:27318202-27318221 MS.gene045581:intron 25.0%
!! AACAAGATTACTAACCTTTT+TGG + chr4.1:27317682-27317701 None:intergenic 25.0%
AACCTTTCAATATCATGGAA+AGG - chr4.1:27317965-27317984 MS.gene045581:CDS 30.0%
ATGATCAAAGGTAAAAAAGG+AGG - chr4.1:27318259-27318278 MS.gene045581:intron 30.0%
CAGTTAACCTTTCAATATCA+TGG - chr4.1:27317960-27317979 MS.gene045581:CDS 30.0%
GAACAATTGAGTGAGTTTAT+TGG - chr4.1:27318499-27318518 MS.gene045581:CDS 30.0%
GCAATGATCAAAGGTAAAAA+AGG - chr4.1:27318256-27318275 MS.gene045581:intron 30.0%
TACAATGTCAAAATCAGCTA+TGG - chr4.1:27318342-27318361 MS.gene045581:CDS 30.0%
TGATTCAATGTCAGATTCTT+TGG + chr4.1:27317939-27317958 None:intergenic 30.0%
TGTTGAACATTATGTCCAAT+TGG + chr4.1:27317991-27318010 None:intergenic 30.0%
! ATGAATGCAAGTTTGCTTAA+AGG - chr4.1:27318523-27318542 MS.gene045581:CDS 30.0%
! CTCAAAATTTAGCTCCAAAA+AGG - chr4.1:27317665-27317684 MS.gene045581:CDS 30.0%
! GTTTCGATATTTAGGTTCAT+CGG - chr4.1:27318095-27318114 MS.gene045581:CDS 30.0%
! TGAATGCAAGTTTGCTTAAA+GGG - chr4.1:27318524-27318543 MS.gene045581:CDS 30.0%
!!! GAAATGTTTTTAAGGCTACT+AGG - chr4.1:27317752-27317771 MS.gene045581:CDS 30.0%
AAGCATAACAACACTTGACA+TGG - chr4.1:27318165-27318184 MS.gene045581:intron 35.0%
AATATCATGGAAAGGCCAAT+TGG - chr4.1:27317973-27317992 MS.gene045581:CDS 35.0%
ATGTCAAAATCAGCTATGGA+TGG - chr4.1:27318346-27318365 MS.gene045581:CDS 35.0%
GACATAATGTTCAACAATGC+AGG - chr4.1:27317995-27318014 MS.gene045581:CDS 35.0%
GCTCATAATCTTTCAGTTGA+TGG - chr4.1:27318619-27318638 MS.gene045581:intron 35.0%
TCTACATTCTGAATCATACG+TGG + chr4.1:27317643-27317662 None:intergenic 35.0%
! ATTTAGGTTCATCGGATCAT+TGG - chr4.1:27318103-27318122 MS.gene045581:CDS 35.0%
!! AATCTCTTTGGAACAATCCA+TGG - chr4.1:27318214-27318233 MS.gene045581:intron 35.0%
!! CATAATCTTTCAGTTGATGG+TGG - chr4.1:27318622-27318641 MS.gene045581:intron 35.0%
!!! GTTTTTAAGGCTACTAGGTA+AGG - chr4.1:27317757-27317776 MS.gene045581:CDS 35.0%
!!! TTTTTGGCTAGTGATGAATC+TGG - chr4.1:27318586-27318605 MS.gene045581:intron 35.0%
AAGGTAGCAGTTATAACAGG+TGG - chr4.1:27317776-27317795 MS.gene045581:CDS 40.0%
AGGGTTGCTGAATCAATTGA+TGG - chr4.1:27317890-27317909 MS.gene045581:CDS 40.0%
GCTGCAAAAGCAATGATCAA+AGG - chr4.1:27318247-27318266 MS.gene045581:intron 40.0%
GGCCTTTCCATGATATTGAA+AGG + chr4.1:27317970-27317989 None:intergenic 40.0%
GGTAAGGTAGCAGTTATAAC+AGG - chr4.1:27317773-27317792 MS.gene045581:CDS 40.0%
GTTAATTGTGTTTCTCCGCA+CGG - chr4.1:27318415-27318434 MS.gene045581:CDS 40.0%
TCCTTCATAGCCTGCAATTA+CGG + chr4.1:27318143-27318162 None:intergenic 40.0%
TTCAACAATGCAGGAATTGC+AGG - chr4.1:27318004-27318023 MS.gene045581:CDS 40.0%
TTGCAGCATGTTTGATTCCA+TGG + chr4.1:27318234-27318253 None:intergenic 40.0%
! AACAACTGATGATGTTGCAC+AGG - chr4.1:27318555-27318574 MS.gene045581:intron 40.0%
! ATCAATTGATTCAGCAACCC+TGG + chr4.1:27317891-27317910 None:intergenic 40.0%
! GCTGATGTTCTAGATGAAGA+AGG - chr4.1:27317863-27317882 MS.gene045581:CDS 40.0%
!! GCATTCAAAAGCATCTCAGA+AGG + chr4.1:27318445-27318464 None:intergenic 40.0%
!! GGATGGATTAATGAGAAGTG+CGG - chr4.1:27318363-27318382 MS.gene045581:CDS 40.0%
ACAATGCAGGAATTGCAGGT+AGG - chr4.1:27318008-27318027 MS.gene045581:CDS 45.0%
CCTTCATAGCCTGCAATTAC+GGG + chr4.1:27318142-27318161 None:intergenic 45.0%
GTTATAACAGGTGGTGCAAG+AGG - chr4.1:27317785-27317804 MS.gene045581:CDS 45.0%
TCAAGTGCAGCTGCAACTAT+AGG - chr4.1:27318298-27318317 MS.gene045581:intron 45.0%
! ATAGGTGGATTCGCTTCACA+TGG - chr4.1:27318316-27318335 MS.gene045581:CDS 45.0%
! GCAAGTTTGCTTAAAGGGAG+AGG - chr4.1:27318529-27318548 MS.gene045581:CDS 45.0%
! GGTTCATCGGATCATTGGTT+TGG - chr4.1:27318108-27318127 MS.gene045581:CDS 45.0%
! TGCACAGGCTGTTCTGTTTT+TGG - chr4.1:27318570-27318589 MS.gene045581:intron 45.0%
!! TCTAGATGAAGAAGGCACCA+GGG - chr4.1:27317871-27317890 MS.gene045581:CDS 45.0%
!! TTCTAGATGAAGAAGGCACC+AGG - chr4.1:27317870-27317889 MS.gene045581:CDS 45.0%
ACAGGTGGTGCAAGAGGAAT+TGG - chr4.1:27317791-27317810 MS.gene045581:CDS 50.0%
AGTGCAGCTGCAACTATAGG+TGG - chr4.1:27318301-27318320 MS.gene045581:intron 50.0%
CCCGTAATTGCAGGCTATGA+AGG - chr4.1:27318139-27318158 MS.gene045581:CDS 50.0%
GGAATTGCAGGTAGGTGCAA+TGG - chr4.1:27318016-27318035 MS.gene045581:CDS 50.0%
GTTCTAACGCCCGTAATTGC+AGG - chr4.1:27318130-27318149 MS.gene045581:CDS 50.0%
TGCAGGTAGGTGCAATGGAA+TGG - chr4.1:27318021-27318040 MS.gene045581:CDS 50.0%
! AGAAGTGCGGCGTGTGAATT+AGG - chr4.1:27318376-27318395 MS.gene045581:CDS 50.0%
ATCTCAGAAGGAACGCCGTG+CGG + chr4.1:27318433-27318452 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 27317634 27318696 27317634 ID=MS.gene045581
chr4.1 mRNA 27317634 27318696 27317634 ID=MS.gene045581.t1;Parent=MS.gene045581
chr4.1 exon 27318644 27318696 27318644 ID=MS.gene045581.t1.exon1;Parent=MS.gene045581.t1
chr4.1 CDS 27318644 27318696 27318644 ID=cds.MS.gene045581.t1;Parent=MS.gene045581.t1
chr4.1 exon 27318305 27318564 27318305 ID=MS.gene045581.t1.exon2;Parent=MS.gene045581.t1
chr4.1 CDS 27318305 27318564 27318305 ID=cds.MS.gene045581.t1;Parent=MS.gene045581.t1
chr4.1 exon 27317634 27318178 27317634 ID=MS.gene045581.t1.exon3;Parent=MS.gene045581.t1
chr4.1 CDS 27317634 27318178 27317634 ID=cds.MS.gene045581.t1;Parent=MS.gene045581.t1
Gene Sequence

>MS.gene045581

ATGGAGCCACGTATGATTCAGAATGTAGAATCTCAAAATTTAGCTCCAAAAAGGTTAGTAATCTTGTTGATATATAGTCTCATTTACCTATCATATGATATTAAATTTCTTTTCTTTTGAAATGTTTTTAAGGCTACTAGGTAAGGTAGCAGTTATAACAGGTGGTGCAAGAGGAATTGGTGCTGCAACAGCAAAATTATTTGCAGAAAATGGAGCACATGTCATCATTGCTGATGTTCTAGATGAAGAAGGCACCAGGGTTGCTGAATCAATTGATGGTCTCTACATACACTGTGATGTATCCAAAGAATCTGACATTGAATCAGCAGTTAACCTTTCAATATCATGGAAAGGCCAATTGGACATAATGTTCAACAATGCAGGAATTGCAGGTAGGTGCAATGGAATGGTTTATTTTGAATTTTGGAAGAAAAAAAAATGGAATTAATTTATATAGTTTTGTTTCGATATTTAGGTTCATCGGATCATTGGTTTGGTTCTAACGCCCGTAATTGCAGGCTATGAAGGAAGAAGCATAACAACACTTGACATGGAAAAACTGACTCATTTACTTTCAATCAATCTCTTTGGAACAATCCATGGAATCAAACATGCTGCAAAAGCAATGATCAAAGGTAAAAAAGGAGGATCAATTATTTGTACATCAAGTGCAGCTGCAACTATAGGTGGATTCGCTTCACATGGTTATACAATGTCAAAATCAGCTATGGATGGATTAATGAGAAGTGCGGCGTGTGAATTAGGCGTTCATTTGATTCGTGTTAATTGTGTTTCTCCGCACGGCGTTCCTTCTGAGATGCTTTTGAATGCTTTTAAATGTTACGGTGAAGTTGATATGACTAGTGAACAATTGAGTGAGTTTATTGGTATGAATGCAAGTTTGCTTAAAGGGAGAGGTGCAACAACTGATGATGTTGCACAGGCTGTTCTGTTTTTGGCTAGTGATGAATCTGGATTTGTAACTGCTCATAATCTTTCAGTTGATGGTGGAATCACATCTGCTAATAGTGTTATGAGTTTCATCTACCAAGATCCTAAGTGA
Protein sequence

>MS.gene045581.t1

MEPRMIQNVESQNLAPKRLLGKVAVITGGARGIGAATAKLFAENGAHVIIADVLDEEGTRVAESIDGLYIHCDVSKESDIESAVNLSISWKGQLDIMFNNAGIAGYEGRSITTLDMEKLTHLLSINLFGTIHGIKHAAKAMIKGKKGGSIICTSSAAATIGGFASHGYTMSKSAMDGLMRSAACELGVHLIRVNCVSPHGVPSEMLLNAFKCYGEVDMTSEQLSEFIGMNASLLKGRGATTDDVAQAVLFLASDESGFVTAHNLSVDGGITSANSVMSFIYQDPK