AlfalfaGEDB | MS.gene23019

M. sativa cultivar XinJiangDaYe / MS.gene23019

KEGG: -
GO: GO:0003674 GO:0003824 GO:0016491
PFAM: PF13561.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene23019.t1	XP_013450332.1	94.4	266	15	0	5	270	31	296	7.80E-137	496.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene23019.t1	Q94KL7	48.6	257	125	3	4	258	12	263	1.4e-62	241.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene23019.t1	A0A072UF24	94.4	266	15	0	5	270	31	296	5.6e-137	496.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene54952	MS.gene23019	PPI
MS.gene031693	MS.gene23019	PPI
MS.gene23019	MS.gene028938	PPI
MS.gene050432	MS.gene23019	PPI
MS.gene23019	MS.gene003992	PPI
MS.gene23019	MS.gene30551	PPI
MS.gene23019	MS.gene055846	PPI
MS.gene012794	MS.gene23019	PPI
MS.gene23019	MS.gene63300	PPI
MS.gene23019	MS.gene87780	PPI
MS.gene072664	MS.gene23019	PPI
MS.gene28847	MS.gene23019	PPI
MS.gene62194	MS.gene23019	PPI
MS.gene23019	MS.gene73824	PPI
MS.gene63307	MS.gene23019	PPI
MS.gene23019	MS.gene80360	PPI
MS.gene23019	MS.gene008160	PPI
MS.gene006617	MS.gene23019	PPI
MS.gene23019	MS.gene024269	PPI
MS.gene23019	MS.gene003729	PPI
MS.gene54598	MS.gene23019	PPI
MS.gene23019	MS.gene050131	PPI
MS.gene23019	MS.gene43481	PPI
MS.gene23019	MS.gene07097	PPI
MS.gene23019	MS.gene62195	PPI
MS.gene08694	MS.gene23019	PPI
MS.gene003926	MS.gene23019	PPI
MS.gene52742	MS.gene23019	PPI
MS.gene23019	MS.gene95020	PPI
MS.gene52743	MS.gene23019	PPI
MS.gene50522	MS.gene23019	PPI
MS.gene007678	MS.gene23019	PPI
MS.gene044694	MS.gene23019	PPI
MS.gene23019	MS.gene50524	PPI
MS.gene63484	MS.gene23019	PPI
MS.gene008162	MS.gene23019	PPI
MS.gene23019	MS.gene43514	PPI
MS.gene021164	MS.gene23019	PPI
MS.gene015282	MS.gene23019	PPI
MS.gene23019	MS.gene63482	PPI
MS.gene63483	MS.gene23019	PPI
MS.gene065380	MS.gene23019	PPI
MS.gene09624	MS.gene23019	PPI
MS.gene23019	MS.gene04700	PPI
MS.gene23019	MS.gene31507	PPI
MS.gene42856	MS.gene23019	PPI
MS.gene23019	MS.gene08695	PPI
MS.gene63403	MS.gene23019	PPI
MS.gene019786	MS.gene23019	PPI
MS.gene23019	MS.gene008161	PPI
MS.gene85724	MS.gene23019	PPI
MS.gene41122	MS.gene23019	PPI
MS.gene63306	MS.gene23019	PPI
MS.gene62187	MS.gene23019	PPI
MS.gene63308	MS.gene23019	PPI
MS.gene033685	MS.gene23019	PPI
MS.gene23019	MS.gene03319	PPI
MS.gene23019	MS.gene043387	PPI
MS.gene045581	MS.gene23019	PPI
MS.gene018066	MS.gene23019	PPI
MS.gene23019	MS.gene008153	PPI
MS.gene28848	MS.gene23019	PPI
MS.gene87026	MS.gene23019	PPI
MS.gene050431	MS.gene23019	PPI
MS.gene003925	MS.gene23019	PPI
MS.gene23019	MS.gene08756	PPI
MS.gene62193	MS.gene23019	PPI
MS.gene23019	MS.gene42114	PPI
MS.gene73088	MS.gene23019	PPI
MS.gene23019	MS.gene039828	PPI
MS.gene073684	MS.gene23019	PPI
MS.gene056339	MS.gene23019	PPI
MS.gene07096	MS.gene23019	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene23019.t1	MTR_7g107670	93.657	268	17	0	5	272	31	298	0.0	518
MS.gene23019.t1	MTR_3g113870	91.827	208	17	0	13	220	27	234	1.57e-138	390
MS.gene23019.t1	MTR_7g083870	56.343	268	116	1	5	271	33	300	7.36e-110	320
MS.gene23019.t1	MTR_4g052350	50.965	259	118	3	5	262	12	262	7.86e-84	252
MS.gene23019.t1	MTR_7g071680	44.853	272	136	4	5	262	27	298	2.03e-81	247
MS.gene23019.t1	MTR_4g052340	48.864	264	119	4	5	260	12	267	6.90e-80	242
MS.gene23019.t1	MTR_4g052320	49.225	258	123	3	6	262	7	257	1.33e-79	241
MS.gene23019.t1	MTR_4g052300	48.837	258	123	3	6	262	7	256	1.06e-76	234
MS.gene23019.t1	MTR_7g105460	48.031	254	126	2	6	258	12	260	8.80e-73	224
MS.gene23019.t1	MTR_7g110130	43.750	256	137	3	5	258	4	254	2.85e-71	220
MS.gene23019.t1	MTR_2g090165	44.531	256	134	4	5	258	4	253	5.24e-70	217
MS.gene23019.t1	MTR_7g105580	44.747	257	118	3	4	259	5	238	2.86e-69	214
MS.gene23019.t1	MTR_4g078630	44.402	259	137	5	5	259	17	272	9.44e-69	214
MS.gene23019.t1	MTR_3g020670	45.349	258	138	2	4	258	13	270	8.40e-67	209
MS.gene23019.t1	MTR_7g110140	43.609	266	142	4	6	269	5	264	9.13e-66	206
MS.gene23019.t1	MTR_6g035395	42.205	263	133	5	3	264	1	245	7.97e-64	201
MS.gene23019.t1	MTR_6g023590	40.541	259	146	4	6	261	12	265	1.95e-63	200
MS.gene23019.t1	MTR_6g023990	41.176	255	142	4	6	257	13	262	5.48e-62	196
MS.gene23019.t1	MTR_6g023910	40.458	262	148	4	4	262	10	266	5.54e-62	196
MS.gene23019.t1	MTR_7g110160	42.188	256	122	4	5	258	4	235	1.19e-60	192
MS.gene23019.t1	MTR_6g023950	41.569	255	141	4	6	257	12	261	2.63e-60	192
MS.gene23019.t1	MTR_6g023610	39.231	260	148	5	6	261	12	265	1.08e-58	188
MS.gene23019.t1	MTR_7g105590	50.532	188	92	1	5	191	6	193	2.19e-58	185
MS.gene23019.t1	MTR_6g023600	40.613	261	143	5	6	261	12	265	1.43e-56	182
MS.gene23019.t1	MTR_6g023600	39.847	261	139	6	6	261	12	259	2.22e-52	171
MS.gene23019.t1	MTR_7g105570	38.202	267	100	4	2	258	6	217	1.93e-50	166
MS.gene23019.t1	MTR_4g084800	51.493	134	64	1	4	136	10	143	1.18e-39	135
MS.gene23019.t1	MTR_3g085740	30.233	258	163	6	5	257	73	318	7.88e-30	114
MS.gene23019.t1	MTR_4g132850	29.478	268	162	9	1	259	1	250	5.02e-28	108
MS.gene23019.t1	MTR_3g068155	29.658	263	167	8	3	260	17	266	8.16e-25	100
MS.gene23019.t1	MTR_7g094510	28.030	264	168	8	4	257	3	254	6.34e-23	94.7
MS.gene23019.t1	MTR_3g068070	28.462	260	170	8	3	258	13	260	1.06e-22	94.7
MS.gene23019.t1	MTR_1196s0010	29.924	264	144	9	9	257	276	513	1.48e-22	97.1
MS.gene23019.t1	MTR_3g068085	27.907	258	168	7	7	258	15	260	8.25e-22	92.0
MS.gene23019.t1	MTR_3g068065	27.237	257	173	6	7	259	17	263	1.06e-21	92.0
MS.gene23019.t1	MTR_7g094520	26.437	261	176	5	4	257	8	259	4.35e-21	90.1
MS.gene23019.t1	MTR_3g068175	27.099	262	171	7	3	258	15	262	4.79e-21	90.1
MS.gene23019.t1	MTR_4g064887	26.718	262	172	7	3	258	13	260	1.50e-20	88.6
MS.gene23019.t1	MTR_7g063330	29.658	263	168	7	3	259	13	264	3.30e-20	87.8
MS.gene23019.t1	MTR_4g131740	28.731	268	161	10	4	256	37	289	6.37e-20	87.4
MS.gene23019.t1	MTR_7g113650	25.188	266	172	7	4	256	37	288	3.44e-19	85.5
MS.gene23019.t1	MTR_3g068175	26.692	266	171	8	3	258	15	266	4.10e-19	85.1
MS.gene23019.t1	MTR_4g097510	28.571	196	137	2	8	202	3	196	6.10e-19	84.3
MS.gene23019.t1	MTR_7g063270	29.740	269	160	8	3	259	14	265	9.99e-19	83.6
MS.gene23019.t1	MTR_8g442750	31.579	266	153	11	7	257	14	265	1.61e-18	83.6
MS.gene23019.t1	MTR_1g082760	26.316	266	169	7	4	256	37	288	1.90e-18	83.2
MS.gene23019.t1	MTR_7g063260	29.278	263	170	7	3	259	14	266	3.83e-18	82.0
MS.gene23019.t1	MTR_7g063390	29.389	262	169	8	3	258	14	265	2.22e-17	80.1
MS.gene23019.t1	MTR_6g023940	27.041	196	82	3	6	200	12	147	4.17e-17	76.6
MS.gene23019.t1	MTR_1g051165	26.296	270	173	8	5	258	12	271	5.31e-17	79.3
MS.gene23019.t1	MTR_7g063320	29.119	261	169	8	3	257	13	263	5.96e-17	79.0
MS.gene23019.t1	MTR_7g063360	29.008	262	170	8	3	258	14	265	8.83e-17	78.2
MS.gene23019.t1	MTR_7g080410	30.412	194	125	5	7	192	38	229	1.16e-16	78.6
MS.gene23019.t1	MTR_4g064883	25.385	260	178	8	3	258	12	259	1.20e-16	77.8
MS.gene23019.t1	MTR_7g080410	30.412	194	125	5	7	192	38	229	1.61e-16	78.2
MS.gene23019.t1	MTR_4g058810	29.167	264	164	9	7	258	11	263	2.65e-16	77.0
MS.gene23019.t1	MTR_1g068955	27.969	261	163	6	7	255	14	261	8.88e-16	75.9
MS.gene23019.t1	MTR_4g058840	28.736	261	169	8	7	258	11	263	2.06e-15	74.7
MS.gene23019.t1	MTR_4g058820	28.736	261	169	8	7	258	11	263	2.06e-15	74.7
MS.gene23019.t1	MTR_4g086400	26.619	278	158	12	12	270	9	259	1.49e-14	73.6
MS.gene23019.t1	MTR_4g064887	27.778	198	131	5	3	194	13	204	2.11e-14	70.9
MS.gene23019.t1	MTR_3g087850	28.415	183	122	4	11	190	30	206	2.62e-14	71.6
MS.gene23019.t1	MTR_4g064883	26.396	197	137	5	3	195	12	204	2.40e-13	67.8
MS.gene23019.t1	MTR_7g105560	55.932	59	22	1	4	62	10	64	6.32e-13	65.5
MS.gene23019.t1	MTR_4g086410	29.231	195	123	5	12	192	9	202	9.94e-13	68.2
MS.gene23019.t1	MTR_3g087860	26.627	169	116	3	24	190	29	191	1.36e-12	66.6
MS.gene23019.t1	MTR_4g134660	30.500	200	129	7	5	197	4	200	2.74e-12	65.1
MS.gene23019.t1	MTR_8g044240	26.667	195	133	4	13	197	75	269	3.51e-12	65.9
MS.gene23019.t1	MTR_4g066050	28.358	201	134	6	5	197	22	220	6.58e-11	61.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene23019.t1	AT4G03140	71.536	267	76	0	5	271	76	342	1.50e-141	401
MS.gene23019.t1	AT3G26770	56.343	268	114	2	4	269	38	304	3.80e-110	320
MS.gene23019.t1	AT3G26760	53.184	267	122	2	4	267	33	299	3.03e-105	308
MS.gene23019.t1	AT3G51680	45.956	272	135	4	3	262	28	299	7.06e-77	236
MS.gene23019.t1	AT2G47130	47.104	259	126	6	6	261	5	255	1.53e-76	233
MS.gene23019.t1	AT2G47120	44.867	263	135	6	1	261	1	255	1.70e-75	231
MS.gene23019.t1	AT2G47120	45.349	258	131	6	6	261	5	254	2.71e-75	230
MS.gene23019.t1	AT3G29250	46.899	258	129	4	6	261	5	256	5.67e-75	229
MS.gene23019.t1	AT3G29250	45.091	275	131	5	1	261	24	292	9.58e-75	230
MS.gene23019.t1	AT3G29250	44.765	277	131	5	1	261	24	294	2.89e-74	229
MS.gene23019.t1	AT3G29260	44.231	260	134	5	5	261	4	255	5.12e-72	222
MS.gene23019.t1	AT2G47140	42.085	259	142	5	5	261	4	256	6.46e-68	211
MS.gene23019.t1	AT1G52340	46.545	275	121	7	5	261	16	282	5.11e-67	210
MS.gene23019.t1	AT3G42960	42.692	260	141	6	5	259	6	262	7.04e-60	191
MS.gene23019.t1	AT2G47150	32.812	256	113	6	7	261	1	198	6.41e-32	117
MS.gene23019.t1	AT1G07450	29.119	261	166	7	3	258	6	252	3.07e-25	101
MS.gene23019.t1	AT4G05530	30.000	260	163	7	5	259	7	252	3.80e-24	98.2
MS.gene23019.t1	AT5G06060	27.692	260	172	6	3	258	7	254	7.33e-24	97.4
MS.gene23019.t1	AT2G29310	28.462	260	170	6	3	258	5	252	1.31e-23	97.1
MS.gene23019.t1	AT5G18210	30.303	264	159	9	7	257	8	259	1.44e-23	96.7
MS.gene23019.t1	AT1G24360	29.070	258	166	7	5	257	72	317	1.92e-23	97.4
MS.gene23019.t1	AT2G29340	28.462	260	170	7	3	258	5	252	1.92e-23	96.3
MS.gene23019.t1	AT1G63380	30.303	264	164	9	6	258	14	268	3.01e-23	96.3
MS.gene23019.t1	AT1G63380	30.303	264	164	9	6	258	16	270	3.18e-23	96.3
MS.gene23019.t1	AT1G63380	30.303	264	164	9	6	258	21	275	3.25e-23	96.3
MS.gene23019.t1	AT1G63380	30.303	264	164	9	6	258	18	272	3.39e-23	96.3
MS.gene23019.t1	AT2G29340	28.462	260	170	7	3	258	5	252	3.61e-23	96.7
MS.gene23019.t1	AT2G30670	28.352	261	169	8	3	258	5	252	2.88e-22	93.2
MS.gene23019.t1	AT2G29330	27.692	260	172	7	3	258	5	252	4.32e-22	92.8
MS.gene23019.t1	AT1G62610	29.545	264	166	9	6	258	12	266	9.12e-22	92.0
MS.gene23019.t1	AT1G62610	29.545	264	166	9	6	258	14	268	9.14e-22	92.4
MS.gene23019.t1	AT1G62610	29.545	264	166	9	6	258	13	267	9.66e-22	92.0
MS.gene23019.t1	AT1G62610	29.545	264	166	9	6	258	18	272	9.76e-22	92.4
MS.gene23019.t1	AT1G54870	28.464	267	162	9	4	256	79	330	2.08e-21	92.4
MS.gene23019.t1	AT1G54870	28.464	267	162	9	4	256	81	332	2.17e-21	92.0
MS.gene23019.t1	AT3G55290	30.827	266	159	9	6	258	16	269	2.68e-21	90.9
MS.gene23019.t1	AT3G55290	30.827	266	159	9	6	258	17	270	2.93e-21	90.9
MS.gene23019.t1	AT2G29360	30.037	273	163	11	3	263	14	270	3.29e-21	90.5
MS.gene23019.t1	AT2G29350	28.413	271	169	8	3	263	13	268	1.20e-20	89.0
MS.gene23019.t1	AT2G17845	29.699	266	161	8	6	256	46	300	1.63e-20	89.4
MS.gene23019.t1	AT2G29370	30.153	262	163	10	3	258	14	261	1.90e-20	88.6
MS.gene23019.t1	AT2G29300	25.843	267	173	8	3	260	5	255	2.13e-20	88.2
MS.gene23019.t1	AT3G46170	30.153	262	166	7	6	258	25	278	2.14e-20	88.6
MS.gene23019.t1	AT2G29300	25.843	267	173	8	3	260	5	255	2.62e-20	88.6
MS.gene23019.t1	AT2G29320	27.444	266	166	8	3	258	11	259	4.73e-20	87.4
MS.gene23019.t1	AT1G07440	27.692	260	171	7	3	258	10	256	5.99e-20	87.0
MS.gene23019.t1	AT3G55310	30.827	266	159	9	6	258	16	269	8.12e-20	87.0
MS.gene23019.t1	AT2G29150	28.077	260	166	8	3	256	14	258	1.54e-19	85.9
MS.gene23019.t1	AT4G13180	28.897	263	167	7	4	257	10	261	1.87e-19	85.5
MS.gene23019.t1	AT5G18210	29.924	264	147	10	7	257	8	246	3.24e-19	85.1
MS.gene23019.t1	AT2G29290	26.923	260	174	6	3	258	5	252	1.25e-18	83.6
MS.gene23019.t1	AT3G03980	27.820	266	167	7	7	257	14	269	1.83e-18	83.2
MS.gene23019.t1	AT2G29260	27.675	271	178	9	3	269	66	322	3.13e-18	83.2
MS.gene23019.t1	AT1G10310	31.579	190	126	3	10	197	18	205	3.96e-18	81.6
MS.gene23019.t1	AT3G05260	26.119	268	168	7	4	257	34	285	4.94e-18	82.0
MS.gene23019.t1	AT2G29340	29.146	199	133	5	3	197	5	199	8.25e-18	79.7
MS.gene23019.t1	AT2G29340	29.146	199	133	5	3	197	5	199	8.25e-18	79.7
MS.gene23019.t1	AT3G04000	26.937	271	162	6	7	256	14	269	8.67e-18	81.3
MS.gene23019.t1	AT3G12800	29.562	274	167	8	7	266	10	271	8.38e-17	79.0
MS.gene23019.t1	AT2G29310	28.643	199	134	4	3	197	5	199	1.03e-16	77.0
MS.gene23019.t1	AT2G29310	26.087	230	156	5	33	258	31	250	3.41e-16	76.6
MS.gene23019.t1	AT2G29350	29.293	198	128	5	3	194	13	204	6.68e-16	75.1
MS.gene23019.t1	AT1G49670	31.122	196	118	5	12	192	9	202	1.10e-15	77.0
MS.gene23019.t1	AT1G49670	31.122	196	118	5	12	192	9	202	1.25e-15	77.0
MS.gene23019.t1	AT2G29350	26.638	229	150	6	40	263	15	230	3.62e-15	73.2
MS.gene23019.t1	AT3G47350	27.778	198	136	5	10	203	47	241	1.93e-14	72.4
MS.gene23019.t1	AT3G47350	28.788	198	134	6	10	203	47	241	2.39e-14	72.0
MS.gene23019.t1	AT5G04900	27.320	194	132	3	13	197	83	276	9.83e-14	70.5
MS.gene23019.t1	AT5G65205	29.032	186	123	4	8	190	7	186	1.10e-13	70.1
MS.gene23019.t1	AT2G29290	24.545	220	153	5	40	258	28	235	1.73e-12	65.9
MS.gene23019.t1	AT3G03330	26.289	194	134	4	7	192	41	233	8.53e-12	64.7
MS.gene23019.t1	AT5G19200	30.918	207	121	9	13	208	41	236	2.31e-11	63.5
MS.gene23019.t1	AT3G06060	28.866	194	125	4	7	193	37	224	5.25e-11	62.4
MS.gene23019.t1	AT3G06060	28.866	194	125	4	7	193	54	241	6.49e-11	62.4

Find 61 sgRNAs with CRISPR-Local

Find 65 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GTTCACAATTAGCTCCTTTC+AGG	0.179729	7.2:+6853822	None:intergenic
GACAAGGTAGCACTGATAAC+TGG	0.262621	7.2:-6854457	MS.gene23019:CDS
GTATATCAGCGGGCATAATT+TGG	0.276919	7.2:-6853750	MS.gene23019:CDS
TACCTTGTCTTGTAGCTTCC+TGG	0.278831	7.2:+6854471	None:intergenic
GCAACAAAATTCATCAATAA+TGG	0.333166	7.2:-6854406	MS.gene23019:CDS
AATAAATGTTCGCGGCGTTA+TGG	0.349280	7.2:-6854140	MS.gene23019:CDS
GTATCCAAGTTTGCGGTTAT+AGG	0.351960	7.2:-6854007	MS.gene23019:CDS
GTAATGATCCCGCGTGGAAC+TGG	0.374917	7.2:-6854094	MS.gene23019:CDS
CTATGCACTGCCAGCGTCAC+TGG	0.405254	7.2:-6854064	MS.gene23019:CDS
TTTCTTGGCCAAGTTGTTGT+TGG	0.415820	7.2:+6854359	None:intergenic
TCTTTCTAATGCACTTGATC+TGG	0.425144	7.2:+6853680	None:intergenic
AATGCCTATAACCGCAAACT+TGG	0.438642	7.2:+6854003	None:intergenic
CCATGGCTTCAGAGCTATCC+AGG	0.442092	7.2:-6853973	MS.gene23019:CDS
CACGTCTGATGTGGTGGTCC+AGG	0.444410	7.2:-6854489	None:intergenic
ATTGACTCGGATTCCATGCC+TGG	0.445621	7.2:+6853955	None:intergenic
GATATCATGTATAACAATGC+AGG	0.475659	7.2:-6854229	MS.gene23019:CDS
CATAATTTGGTTGTGGATGG+AGG	0.480867	7.2:-6853737	MS.gene23019:CDS
GTAGAAATTGTTCGTAATGT+TGG	0.482699	7.2:-6853848	MS.gene23019:CDS
TATAGGCATTGTTAAATCCA+TGG	0.486525	7.2:-6853990	MS.gene23019:CDS
AGATTGAGGTCAACAACGCT+TGG	0.496330	7.2:+6854187	None:intergenic
GCCAGCGTCACTGGTGTGAT+TGG	0.498683	7.2:-6854055	MS.gene23019:CDS
TAATGCACTTGATCTGGTGC+AGG	0.501427	7.2:+6853686	None:intergenic
TCCATTTCGCTCATGACAAA+AGG	0.502082	7.2:+6853905	None:intergenic
CAGCGGGCATAATTTGGTTG+TGG	0.504711	7.2:-6853744	MS.gene23019:CDS
ATACGCTGTATCCAAGTTTG+CGG	0.507901	7.2:-6854014	MS.gene23019:CDS
ACAAGGTAGCACTGATAACT+GGG	0.508049	7.2:-6854456	MS.gene23019:CDS
AAGTTGTTTGTATTCAGAAA+CGG	0.514499	7.2:+6854252	None:intergenic
ACCTTGCATCTAATGATGCT+AGG	0.517372	7.2:-6853772	MS.gene23019:CDS
CACTGGTGTGATTGGAGGGA+TGG	0.517491	7.2:-6854047	MS.gene23019:CDS
TTCATTCACGTCTGATGTGG+TGG	0.518082	7.2:-6854495	None:intergenic
GACGCTGGCAGTGCATAGAA+TGG	0.522764	7.2:+6854069	None:intergenic
CCTGGATAGCTCTGAAGCCA+TGG	0.523976	7.2:+6853973	None:intergenic
TGTCAAACGATTCTAGATTG+AGG	0.524531	7.2:+6854173	None:intergenic
CAAGAAACTGCAAAAGAACT+AGG	0.526436	7.2:-6854343	MS.gene23019:CDS
TGTGACGTCGCAAGTTATGA+AGG	0.529384	7.2:+6854309	None:intergenic
AGCGTCACTGGTGTGATTGG+AGG	0.541442	7.2:-6854052	MS.gene23019:CDS
GCTGATATCCAACAACAACT+TGG	0.550507	7.2:-6854367	MS.gene23019:CDS
TTGAGGTCAACAACGCTTGG+AGG	0.555513	7.2:+6854190	None:intergenic
GGGCATAATTTGGTTGTGGA+TGG	0.562134	7.2:-6853740	MS.gene23019:CDS
ACCTAGCATCATTAGATGCA+AGG	0.564196	7.2:+6853771	None:intergenic
TCCAATCACACCAGTGACGC+TGG	0.565675	7.2:+6854054	None:intergenic
AAAGGAGTAGGAATAGCAAA+AGG	0.575525	7.2:+6853923	None:intergenic
TGCAGCATTAGCGATATCAT+CGG	0.580679	7.2:+6853799	None:intergenic
GCAGCATTAGCGATATCATC+GGG	0.582609	7.2:+6853800	None:intergenic
GTCATGGAAATAAATGTTCG+CGG	0.584404	7.2:-6854148	MS.gene23019:CDS
CGTAATGTTGGTGTCCTGAA+AGG	0.587683	7.2:-6853836	MS.gene23019:CDS
GCGTCACTGGTGTGATTGGA+GGG	0.588989	7.2:-6854051	MS.gene23019:CDS
ATATCATGTATAACAATGCA+GGG	0.592185	7.2:-6854228	MS.gene23019:CDS
TCGCTCATGACAAAAGGAGT+AGG	0.599202	7.2:+6853911	None:intergenic
AGAATCGTTTGACAAAGTCA+TGG	0.604965	7.2:-6854164	MS.gene23019:CDS
GCTTCAGAGCTATCCAGGCA+TGG	0.629348	7.2:-6853968	MS.gene23019:CDS
ATGATGCTAGGTATATCAGC+GGG	0.660569	7.2:-6853760	MS.gene23019:CDS
GTCCAGGAAGCTACAAGACA+AGG	0.662037	7.2:-6854473	MS.gene23019:CDS
AAGGCGATATACAATTGACT+CGG	0.673213	7.2:+6853942	None:intergenic
AGTCGCTGCGAATCAAGATG+TGG	0.674277	7.2:+6853872	None:intergenic
GAATGGATCCAGTTCCACGC+GGG	0.678631	7.2:+6854086	None:intergenic
AATGATGCTAGGTATATCAG+CGG	0.703024	7.2:-6853761	MS.gene23019:CDS
AAGGTAGCACTGATAACTGG+GGG	0.709904	7.2:-6854454	MS.gene23019:CDS
CAAGGTAGCACTGATAACTG+GGG	0.710678	7.2:-6854455	MS.gene23019:CDS
GCGCGTGTAATGATCCCGCG+TGG	0.726381	7.2:-6854100	MS.gene23019:CDS
AGAATGGATCCAGTTCCACG+CGG	0.782409	7.2:+6854085	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTCACATCTTTTAAGAATT+TGG	-	chr7.2:6854452-6854471	MS.gene23019:CDS	20.0%
!	AAGTTGTTTGTATTCAGAAA+CGG	+	chr7.2:6853917-6853936	None:intergenic	25.0%
!	ATATCATGTATAACAATGCA+GGG	-	chr7.2:6853938-6853957	MS.gene23019:CDS	25.0%
!	GCAACAAAATTCATCAATAA+TGG	-	chr7.2:6853760-6853779	MS.gene23019:CDS	25.0%
	GATATCATGTATAACAATGC+AGG	-	chr7.2:6853937-6853956	MS.gene23019:CDS	30.0%
	GTAGAAATTGTTCGTAATGT+TGG	-	chr7.2:6854318-6854337	MS.gene23019:CDS	30.0%
!	TATAGGCATTGTTAAATCCA+TGG	-	chr7.2:6854176-6854195	MS.gene23019:CDS	30.0%
	AAAGGAGTAGGAATAGCAAA+AGG	+	chr7.2:6854246-6854265	None:intergenic	35.0%
	AAGGCGATATACAATTGACT+CGG	+	chr7.2:6854227-6854246	None:intergenic	35.0%
	AATGATGCTAGGTATATCAG+CGG	-	chr7.2:6854405-6854424	MS.gene23019:CDS	35.0%
	AGAATCGTTTGACAAAGTCA+TGG	-	chr7.2:6854002-6854021	MS.gene23019:CDS	35.0%
	CAAGAAACTGCAAAAGAACT+AGG	-	chr7.2:6853823-6853842	MS.gene23019:CDS	35.0%
	GTCATGGAAATAAATGTTCG+CGG	-	chr7.2:6854018-6854037	MS.gene23019:CDS	35.0%
	TGTCAAACGATTCTAGATTG+AGG	+	chr7.2:6853996-6854015	None:intergenic	35.0%
!!	CTAGTTCTTTTGCAGTTTCT+TGG	+	chr7.2:6853825-6853844	None:intergenic	35.0%
	AATAAATGTTCGCGGCGTTA+TGG	-	chr7.2:6854026-6854045	MS.gene23019:CDS	40.0%
	AATGCCTATAACCGCAAACT+TGG	+	chr7.2:6854166-6854185	None:intergenic	40.0%
	ACCTAGCATCATTAGATGCA+AGG	+	chr7.2:6854398-6854417	None:intergenic	40.0%
	ACCTTGCATCTAATGATGCT+AGG	-	chr7.2:6854394-6854413	MS.gene23019:CDS	40.0%
	ATACGCTGTATCCAAGTTTG+CGG	-	chr7.2:6854152-6854171	MS.gene23019:CDS	40.0%
	ATGATGCTAGGTATATCAGC+GGG	-	chr7.2:6854406-6854425	MS.gene23019:CDS	40.0%
	CATAATTTGGTTGTGGATGG+AGG	-	chr7.2:6854429-6854448	MS.gene23019:CDS	40.0%
	GCTGATATCCAACAACAACT+TGG	-	chr7.2:6853799-6853818	MS.gene23019:CDS	40.0%
	GTATATCAGCGGGCATAATT+TGG	-	chr7.2:6854416-6854435	MS.gene23019:CDS	40.0%
	GTATCCAAGTTTGCGGTTAT+AGG	-	chr7.2:6854159-6854178	MS.gene23019:CDS	40.0%
	GTTCACAATTAGCTCCTTTC+AGG	+	chr7.2:6854347-6854366	None:intergenic	40.0%
	TCCATTTCGCTCATGACAAA+AGG	+	chr7.2:6854264-6854283	None:intergenic	40.0%
	TGCAGCATTAGCGATATCAT+CGG	+	chr7.2:6854370-6854389	None:intergenic	40.0%
	TTTCTTGGCCAAGTTGTTGT+TGG	+	chr7.2:6853810-6853829	None:intergenic	40.0%
!	TCCTTTTGTCATGAGCGAAA+TGG	-	chr7.2:6854260-6854279	MS.gene23019:CDS	40.0%
!!	ACAAGGTAGCACTGATAACT+GGG	-	chr7.2:6853710-6853729	MS.gene23019:CDS	40.0%
	AGATTGAGGTCAACAACGCT+TGG	+	chr7.2:6853982-6854001	None:intergenic	45.0%
	GCAGCATTAGCGATATCATC+GGG	+	chr7.2:6854369-6854388	None:intergenic	45.0%
	GGGCATAATTTGGTTGTGGA+TGG	-	chr7.2:6854426-6854445	MS.gene23019:CDS	45.0%
	TAATGCACTTGATCTGGTGC+AGG	+	chr7.2:6854483-6854502	None:intergenic	45.0%
	TACCTTGTCTTGTAGCTTCC+TGG	+	chr7.2:6853698-6853717	None:intergenic	45.0%
	TCGCTCATGACAAAAGGAGT+AGG	+	chr7.2:6854258-6854277	None:intergenic	45.0%
	TGTGACGTCGCAAGTTATGA+AGG	+	chr7.2:6853860-6853879	None:intergenic	45.0%
!	CGTAATGTTGGTGTCCTGAA+AGG	-	chr7.2:6854330-6854349	MS.gene23019:CDS	45.0%
!!	AAGGTAGCACTGATAACTGG+GGG	-	chr7.2:6853712-6853731	MS.gene23019:CDS	45.0%
!!	CAAGGTAGCACTGATAACTG+GGG	-	chr7.2:6853711-6853730	MS.gene23019:CDS	45.0%
!!	GACAAGGTAGCACTGATAAC+TGG	-	chr7.2:6853709-6853728	MS.gene23019:CDS	45.0%
	AGAATGGATCCAGTTCCACG+CGG	+	chr7.2:6854084-6854103	None:intergenic	50.0%
	AGTCGCTGCGAATCAAGATG+TGG	+	chr7.2:6854297-6854316	None:intergenic	50.0%
	CAGCGGGCATAATTTGGTTG+TGG	-	chr7.2:6854422-6854441	MS.gene23019:CDS	50.0%
	GTCCAGGAAGCTACAAGACA+AGG	-	chr7.2:6853693-6853712	MS.gene23019:CDS	50.0%
	TTGAGGTCAACAACGCTTGG+AGG	+	chr7.2:6853979-6853998	None:intergenic	50.0%
!	AACGCTTGGAGGAGTTTTGC+AGG	+	chr7.2:6853968-6853987	None:intergenic	50.0%
!	ACGCTTGGAGGAGTTTTGCA+GGG	+	chr7.2:6853967-6853986	None:intergenic	50.0%
!!	ATTGACTCGGATTCCATGCC+TGG	+	chr7.2:6854214-6854233	None:intergenic	50.0%
	ACTGGGGGAGCTAGTGGAAT+AGG	-	chr7.2:6853727-6853746	MS.gene23019:CDS	55.0%
	AGCGTCACTGGTGTGATTGG+AGG	-	chr7.2:6854114-6854133	MS.gene23019:CDS	55.0%
	CACTGGTGTGATTGGAGGGA+TGG	-	chr7.2:6854119-6854138	MS.gene23019:CDS	55.0%
	CCATGGCTTCAGAGCTATCC+AGG	-	chr7.2:6854193-6854212	MS.gene23019:CDS	55.0%
	CTGATAACTGGGGGAGCTAG+TGG	-	chr7.2:6853721-6853740	MS.gene23019:CDS	55.0%
	GAATGGATCCAGTTCCACGC+GGG	+	chr7.2:6854083-6854102	None:intergenic	55.0%
	GACGCTGGCAGTGCATAGAA+TGG	+	chr7.2:6854100-6854119	None:intergenic	55.0%
	GCGTCACTGGTGTGATTGGA+GGG	-	chr7.2:6854115-6854134	MS.gene23019:CDS	55.0%
	GCTTCAGAGCTATCCAGGCA+TGG	-	chr7.2:6854198-6854217	MS.gene23019:CDS	55.0%
	GTAATGATCCCGCGTGGAAC+TGG	-	chr7.2:6854072-6854091	MS.gene23019:CDS	55.0%
	TCCAATCACACCAGTGACGC+TGG	+	chr7.2:6854115-6854134	None:intergenic	55.0%
!!	CCTGGATAGCTCTGAAGCCA+TGG	+	chr7.2:6854196-6854215	None:intergenic	55.0%
	CTATGCACTGCCAGCGTCAC+TGG	-	chr7.2:6854102-6854121	MS.gene23019:CDS	60.0%
	GCCAGCGTCACTGGTGTGAT+TGG	-	chr7.2:6854111-6854130	MS.gene23019:CDS	60.0%
	GCGCGTGTAATGATCCCGCG+TGG	-	chr7.2:6854066-6854085	MS.gene23019:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.2	gene	6853685	6854503	6853685	ID=MS.gene23019
chr7.2	mRNA	6853685	6854503	6853685	ID=MS.gene23019.t1;Parent=MS.gene23019
chr7.2	exon	6853685	6854503	6853685	ID=MS.gene23019.t1.exon1;Parent=MS.gene23019.t1
chr7.2	CDS	6853685	6854503	6853685	ID=cds.MS.gene23019.t1;Parent=MS.gene23019.t1

Gene Sequence

>MS.gene23019

ATGTGGTGGTCCAGGAAGCTACAAGACAAGGTAGCACTGATAACTGGGGGAGCTAGTGGAATAGGAAAAGCTGCAGCAACAAAATTCATCAATAATGGAGCAAAAGTAATTATTGCTGATATCCAACAACAACTTGGCCAAGAAACTGCAAAAGAACTAGGAACTAATGCTACCTTCATAACTTGCGACGTCACAAAAGAATCAGACATATCTGACGCAGTTGATTTCACCGTTTCTGAATACAAACAACTTGATATCATGTATAACAATGCAGGGATACCCTGCAAAACTCCTCCAAGCGTTGTTGACCTCAATCTAGAATCGTTTGACAAAGTCATGGAAATAAATGTTCGCGGCGTTATGGCTGTCATCAAGCATGCAGCGCGTGTAATGATCCCGCGTGGAACTGGATCCATTCTATGCACTGCCAGCGTCACTGGTGTGATTGGAGGGATGGCGCAGCATACATACGCTGTATCCAAGTTTGCGGTTATAGGCATTGTTAAATCCATGGCTTCAGAGCTATCCAGGCATGGAATCCGAGTCAATTGTATATCGCCTTTTGCTATTCCTACTCCTTTTGTCATGAGCGAAATGGATCAGATATATCCACATCTTGATTCGCAGCGACTTGTAGAAATTGTTCGTAATGTTGGTGTCCTGAAAGGAGCTAATTGTGAACCCGATGATATCGCTAATGCTGCACTTTACCTTGCATCTAATGATGCTAGGTATATCAGCGGGCATAATTTGGTTGTGGATGGAGGTTTCACATCTTTTAAGAATTTGGAGTTTCCTGCACCAGATCAAGTGCATTAG

Protein sequence

>MS.gene23019.t1

MWWSRKLQDKVALITGGASGIGKAAATKFINNGAKVIIADIQQQLGQETAKELGTNATFITCDVTKESDISDAVDFTVSEYKQLDIMYNNAGIPCKTPPSVVDLNLESFDKVMEINVRGVMAVIKHAARVMIPRGTGSILCTASVTGVIGGMAQHTYAVSKFAVIGIVKSMASELSRHGIRVNCISPFAIPTPFVMSEMDQIYPHLDSQRLVEIVRNVGVLKGANCEPDDIANAALYLASNDARYISGHNLVVDGGFTSFKNLEFPAPDQVH