AlfalfaGEDB | MS.gene014425

M. sativa cultivar XinJiangDaYe / MS.gene014425

KEGG: -
GO: GO:0003674 GO:0003824 GO:0016491
PFAM: PF13561.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene014425.t1	XP_013450332.1	92.4	264	20	0	59	322	32	295	6.20E-133	483.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene014425.t1	F1SWA0	48.2	257	130	2	58	311	1	257	3.8e-62	240.0

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene014425.t1	A0A072UF24	92.4	264	20	0	59	322	32	295	4.5e-133	483.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene73088	MS.gene014425	PPI
MS.gene014425	MS.gene028938	PPI
MS.gene014425	MS.gene003992	PPI
MS.gene056339	MS.gene014425	PPI
MS.gene031693	MS.gene014425	PPI
MS.gene014425	MS.gene055846	PPI
MS.gene014425	MS.gene62195	PPI
MS.gene08694	MS.gene014425	PPI
MS.gene003926	MS.gene014425	PPI
MS.gene52742	MS.gene014425	PPI
MS.gene52743	MS.gene014425	PPI
MS.gene014425	MS.gene73824	PPI
MS.gene50522	MS.gene014425	PPI
MS.gene014425	MS.gene024269	PPI
MS.gene014425	MS.gene003729	PPI
MS.gene007678	MS.gene014425	PPI
MS.gene014425	MS.gene050131	PPI
MS.gene014425	MS.gene43481	PPI
MS.gene044694	MS.gene014425	PPI
MS.gene63484	MS.gene014425	PPI
MS.gene014425	MS.gene43514	PPI
MS.gene008162	MS.gene014425	PPI
MS.gene021164	MS.gene014425	PPI
MS.gene014425	MS.gene07097	PPI
MS.gene015282	MS.gene014425	PPI
MS.gene014425	MS.gene31507	PPI
MS.gene014425	MS.gene95020	PPI
MS.gene065380	MS.gene014425	PPI
MS.gene09624	MS.gene014425	PPI
MS.gene42856	MS.gene014425	PPI
MS.gene63403	MS.gene014425	PPI
MS.gene019786	MS.gene014425	PPI
MS.gene014425	MS.gene50524	PPI
MS.gene85724	MS.gene014425	PPI
MS.gene41122	MS.gene014425	PPI
MS.gene63306	MS.gene014425	PPI
MS.gene62187	MS.gene014425	PPI
MS.gene033685	MS.gene014425	PPI
MS.gene014425	MS.gene63482	PPI
MS.gene014425	MS.gene043387	PPI
MS.gene014425	MS.gene04700	PPI
MS.gene014425	MS.gene08695	PPI
MS.gene63483	MS.gene014425	PPI
MS.gene045581	MS.gene014425	PPI
MS.gene014425	MS.gene008161	PPI
MS.gene28848	MS.gene014425	PPI
MS.gene87026	MS.gene014425	PPI
MS.gene050431	MS.gene014425	PPI
MS.gene014425	MS.gene42114	PPI
MS.gene003925	MS.gene014425	PPI
MS.gene62193	MS.gene014425	PPI
MS.gene014425	MS.gene039828	PPI
MS.gene073684	MS.gene014425	PPI
MS.gene014425	MS.gene30551	PPI
MS.gene63308	MS.gene014425	PPI
MS.gene07096	MS.gene014425	PPI
MS.gene54952	MS.gene014425	PPI
MS.gene014425	MS.gene03319	PPI
MS.gene050432	MS.gene014425	PPI
MS.gene014425	MS.gene63300	PPI
MS.gene014425	MS.gene87780	PPI
MS.gene014425	MS.gene008153	PPI
MS.gene012794	MS.gene014425	PPI
MS.gene018066	MS.gene014425	PPI
MS.gene072664	MS.gene014425	PPI
MS.gene014425	MS.gene80360	PPI
MS.gene014425	MS.gene008160	PPI
MS.gene014425	MS.gene08756	PPI
MS.gene28847	MS.gene014425	PPI
MS.gene62194	MS.gene014425	PPI
MS.gene63307	MS.gene014425	PPI
MS.gene006617	MS.gene014425	PPI
MS.gene54598	MS.gene014425	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene014425.t1	MTR_7g107670	92.424	264	20	0	59	322	32	295	2.36e-180	500
MS.gene014425.t1	MTR_3g113870	87.783	221	22	1	53	273	19	234	5.42e-137	388
MS.gene014425.t1	MTR_7g083870	54.513	277	123	2	49	322	22	298	4.99e-107	314
MS.gene014425.t1	MTR_4g052350	49.615	260	122	3	57	315	11	262	1.63e-80	246
MS.gene014425.t1	MTR_7g071680	43.173	271	140	2	59	315	28	298	3.85e-79	243
MS.gene014425.t1	MTR_4g052340	48.077	260	126	3	57	315	11	262	1.36e-78	241
MS.gene014425.t1	MTR_4g052320	48.450	258	125	3	59	315	7	257	1.51e-77	238
MS.gene014425.t1	MTR_4g052300	48.062	258	125	3	59	315	7	256	1.87e-74	230
MS.gene014425.t1	MTR_7g105460	47.674	258	121	3	59	311	12	260	5.83e-70	219
MS.gene014425.t1	MTR_4g078630	43.015	272	146	6	45	312	6	272	8.36e-68	214
MS.gene014425.t1	MTR_7g110130	43.077	260	131	5	59	311	5	254	1.28e-65	207
MS.gene014425.t1	MTR_7g105580	44.314	255	118	3	59	312	7	238	2.62e-65	206
MS.gene014425.t1	MTR_3g020670	45.703	256	136	2	59	311	15	270	5.35e-65	206
MS.gene014425.t1	MTR_7g110140	42.697	267	145	6	58	322	4	264	3.31e-62	199
MS.gene014425.t1	MTR_2g090165	41.569	255	141	4	59	311	5	253	5.93e-62	198
MS.gene014425.t1	MTR_7g105590	53.476	187	86	1	59	244	7	193	3.79e-61	194
MS.gene014425.t1	MTR_6g023590	40.385	260	147	5	58	314	11	265	6.65e-61	195
MS.gene014425.t1	MTR_6g035395	41.923	260	132	5	59	317	4	245	5.20e-60	192
MS.gene014425.t1	MTR_6g023990	40.234	256	145	5	58	310	12	262	8.79e-58	187
MS.gene014425.t1	MTR_6g023910	38.577	267	156	4	52	315	5	266	7.32e-57	185
MS.gene014425.t1	MTR_7g110160	40.784	255	125	4	59	311	5	235	1.14e-56	184
MS.gene014425.t1	MTR_6g023950	41.016	256	143	5	58	310	11	261	6.01e-56	182
MS.gene014425.t1	MTR_6g023610	37.931	261	152	5	58	314	11	265	9.28e-55	180
MS.gene014425.t1	MTR_6g023600	39.231	260	150	5	58	314	11	265	1.34e-53	176
MS.gene014425.t1	MTR_6g023600	38.462	260	146	6	58	314	11	259	2.00e-49	166
MS.gene014425.t1	MTR_7g105570	39.062	256	93	4	60	311	21	217	2.81e-48	162
MS.gene014425.t1	MTR_4g084800	52.273	132	62	1	59	189	12	143	3.01e-39	135
MS.gene014425.t1	MTR_3g085740	28.736	261	169	6	55	310	70	318	1.62e-27	109
MS.gene014425.t1	MTR_4g132850	27.413	259	169	7	59	312	6	250	9.00e-24	98.2
MS.gene014425.t1	MTR_1196s0010	29.771	262	156	8	58	310	271	513	1.14e-22	98.6
MS.gene014425.t1	MTR_3g068155	28.736	261	170	7	58	313	17	266	8.75e-22	93.2
MS.gene014425.t1	MTR_3g068070	28.409	264	163	7	58	311	13	260	1.82e-21	92.4
MS.gene014425.t1	MTR_7g094510	28.517	263	166	9	58	310	4	254	3.42e-21	91.3
MS.gene014425.t1	MTR_3g068065	26.848	257	174	6	60	312	17	263	2.63e-20	89.4
MS.gene014425.t1	MTR_3g068085	27.519	258	169	7	60	311	15	260	2.87e-20	89.0
MS.gene014425.t1	MTR_7g094520	26.641	259	172	6	60	310	11	259	2.86e-18	83.2
MS.gene014425.t1	MTR_4g064887	26.744	258	171	6	60	311	15	260	3.28e-18	83.2
MS.gene014425.t1	MTR_7g063330	28.464	267	176	6	52	312	7	264	7.41e-18	82.0
MS.gene014425.t1	MTR_7g063270	28.676	272	169	6	52	312	8	265	8.31e-18	82.0
MS.gene014425.t1	MTR_7g113650	25.092	271	172	8	54	309	34	288	9.59e-18	82.4
MS.gene014425.t1	MTR_7g063260	28.839	267	176	6	52	312	8	266	1.82e-17	81.3
MS.gene014425.t1	MTR_3g068175	27.308	260	167	7	60	311	17	262	2.66e-17	80.9
MS.gene014425.t1	MTR_8g442750	30.827	266	155	11	60	310	14	265	5.51e-17	80.5
MS.gene014425.t1	MTR_4g131740	27.820	266	162	10	59	309	39	289	7.07e-17	80.1
MS.gene014425.t1	MTR_1g082760	25.379	264	170	7	59	309	39	288	1.81e-16	78.6
MS.gene014425.t1	MTR_7g063390	28.571	266	176	7	52	311	8	265	7.03e-16	76.6
MS.gene014425.t1	MTR_7g063360	28.947	266	175	7	52	311	8	265	1.26e-15	75.9
MS.gene014425.t1	MTR_4g097510	27.041	196	140	2	61	255	3	196	1.29e-15	76.3
MS.gene014425.t1	MTR_3g068175	26.894	264	167	8	60	311	17	266	1.80e-15	75.5
MS.gene014425.t1	MTR_4g064883	25.000	256	178	7	60	311	14	259	2.39e-15	75.1
MS.gene014425.t1	MTR_7g063320	28.309	272	165	8	53	310	8	263	3.56e-15	74.7
MS.gene014425.t1	MTR_1g051165	26.277	274	173	8	54	311	11	271	2.43e-14	72.4
MS.gene014425.t1	MTR_7g080410	29.897	194	126	5	60	245	38	229	2.81e-14	72.8
MS.gene014425.t1	MTR_7g080410	29.897	194	126	5	60	245	38	229	4.21e-14	72.0
MS.gene014425.t1	MTR_3g087850	28.962	183	121	4	64	243	30	206	8.35e-14	71.2
MS.gene014425.t1	MTR_1g068955	26.667	270	159	7	58	308	12	261	2.04e-13	69.7
MS.gene014425.t1	MTR_4g086400	26.007	273	162	10	65	321	9	257	2.07e-13	71.2
MS.gene014425.t1	MTR_4g058810	26.894	264	170	8	60	311	11	263	5.02e-13	68.6
MS.gene014425.t1	MTR_4g086410	29.231	195	123	4	65	245	9	202	7.05e-13	69.7
MS.gene014425.t1	MTR_4g058840	26.820	261	174	7	60	311	11	263	1.35e-12	67.4
MS.gene014425.t1	MTR_4g058820	26.820	261	174	7	60	311	11	263	1.35e-12	67.4
MS.gene014425.t1	MTR_4g064887	27.320	194	131	4	60	247	15	204	2.50e-12	65.9
MS.gene014425.t1	MTR_8g044240	26.289	194	133	4	66	249	75	268	5.89e-12	66.2
MS.gene014425.t1	MTR_7g105560	58.824	51	21	0	59	109	12	62	1.06e-11	62.4
MS.gene014425.t1	MTR_4g064883	25.907	193	137	4	60	248	14	204	1.17e-11	63.5
MS.gene014425.t1	MTR_6g088500	25.701	214	120	4	66	249	184	388	5.08e-11	63.5
MS.gene014425.t1	MTR_6g088500	25.701	214	120	4	66	249	184	388	5.30e-11	63.9
MS.gene014425.t1	MTR_3g087860	26.036	169	117	3	77	243	29	191	9.08e-11	62.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene014425.t1	AT4G03140	70.833	264	77	0	59	322	77	340	1.02e-137	394
MS.gene014425.t1	AT3G26770	54.340	265	120	1	59	322	40	304	4.57e-104	307
MS.gene014425.t1	AT3G26760	52.075	265	124	2	59	320	35	299	5.77e-100	296
MS.gene014425.t1	AT3G29250	43.158	285	141	6	45	314	14	292	5.77e-72	225
MS.gene014425.t1	AT2G47120	44.015	259	135	6	58	314	4	254	7.04e-72	223
MS.gene014425.t1	AT3G29250	45.560	259	133	4	58	314	4	256	9.62e-72	223
MS.gene014425.t1	AT3G29250	43.206	287	140	6	45	314	14	294	1.23e-71	224
MS.gene014425.t1	AT2G47120	43.846	260	136	6	57	314	4	255	1.45e-71	223
MS.gene014425.t1	AT2G47130	44.615	260	133	5	58	314	4	255	3.08e-71	221
MS.gene014425.t1	AT3G51680	44.610	269	137	4	59	315	31	299	5.28e-71	223
MS.gene014425.t1	AT3G29260	42.857	259	137	5	59	314	5	255	6.22e-68	213
MS.gene014425.t1	AT2G47140	40.698	258	145	5	59	314	5	256	1.03e-63	202
MS.gene014425.t1	AT1G52340	45.818	275	121	7	59	314	17	282	2.27e-61	197
MS.gene014425.t1	AT3G42960	42.471	259	141	6	59	312	7	262	9.44e-58	187
MS.gene014425.t1	AT2G47150	31.349	252	114	5	60	310	1	194	2.66e-30	114
MS.gene014425.t1	AT1G07450	28.682	258	169	6	58	311	6	252	4.93e-23	96.3
MS.gene014425.t1	AT1G24360	28.571	259	168	7	57	310	71	317	3.66e-22	95.1
MS.gene014425.t1	AT5G06060	26.493	268	179	6	48	311	1	254	7.48e-22	93.2
MS.gene014425.t1	AT1G63380	29.329	283	177	10	40	311	2	272	1.26e-21	93.2
MS.gene014425.t1	AT2G29310	28.846	260	171	6	56	311	3	252	1.44e-21	92.4
MS.gene014425.t1	AT2G29340	27.273	264	170	5	56	311	3	252	4.40e-21	91.3
MS.gene014425.t1	AT1G63380	29.545	264	166	9	59	311	21	275	6.34e-21	91.3
MS.gene014425.t1	AT2G29340	27.273	264	170	5	56	311	3	252	6.65e-21	91.7
MS.gene014425.t1	AT5G18210	29.213	267	164	9	57	310	5	259	8.84e-21	90.1
MS.gene014425.t1	AT1G63380	29.545	264	166	9	59	311	14	268	9.27e-21	90.5
MS.gene014425.t1	AT1G63380	29.545	264	166	9	59	311	16	270	9.28e-21	90.5
MS.gene014425.t1	AT4G05530	28.958	259	165	7	59	312	8	252	1.00e-20	90.1
MS.gene014425.t1	AT1G62610	27.915	283	181	9	40	311	2	272	3.17e-20	89.4
MS.gene014425.t1	AT1G54870	27.737	274	169	8	50	309	74	332	5.03e-20	89.4
MS.gene014425.t1	AT1G54870	27.737	274	169	8	50	309	72	330	5.06e-20	89.4
MS.gene014425.t1	AT2G30670	27.692	260	174	6	56	311	3	252	1.41e-19	87.0
MS.gene014425.t1	AT2G29330	27.308	260	175	6	56	311	3	252	1.76e-19	86.7
MS.gene014425.t1	AT1G62610	28.788	264	168	9	59	311	14	268	2.02e-19	87.0
MS.gene014425.t1	AT1G62610	28.788	264	168	9	59	311	12	266	2.30e-19	86.7
MS.gene014425.t1	AT1G62610	28.788	264	168	9	59	311	13	267	2.43e-19	86.7
MS.gene014425.t1	AT2G29360	27.915	283	178	9	44	316	4	270	3.20e-19	86.3
MS.gene014425.t1	AT2G29370	28.016	257	169	7	60	311	16	261	5.78e-19	85.5
MS.gene014425.t1	AT2G17845	28.947	266	163	8	59	309	46	300	6.85e-19	85.9
MS.gene014425.t1	AT3G03980	28.467	274	171	7	52	310	6	269	8.61e-19	85.1
MS.gene014425.t1	AT2G29300	25.843	267	175	7	56	313	3	255	1.37e-18	84.7
MS.gene014425.t1	AT3G46170	29.771	262	167	7	59	311	25	278	1.38e-18	84.7
MS.gene014425.t1	AT3G55290	30.451	266	160	9	59	311	16	269	1.60e-18	84.3
MS.gene014425.t1	AT3G55290	30.451	266	160	9	59	311	17	270	1.74e-18	84.3
MS.gene014425.t1	AT2G29300	25.843	267	175	7	56	313	3	255	2.60e-18	83.6
MS.gene014425.t1	AT2G29320	26.966	267	170	7	55	311	8	259	2.85e-18	83.6
MS.gene014425.t1	AT2G29150	27.675	271	165	9	49	309	9	258	5.95e-18	82.4
MS.gene014425.t1	AT2G29350	28.090	267	169	7	60	316	15	268	9.42e-18	82.0
MS.gene014425.t1	AT3G55310	30.827	266	159	9	59	311	16	269	1.63e-17	81.6
MS.gene014425.t1	AT3G04000	26.838	272	161	6	60	309	14	269	3.47e-17	80.5
MS.gene014425.t1	AT5G18210	29.213	267	151	10	57	310	5	246	8.00e-17	79.7
MS.gene014425.t1	AT2G29260	26.966	267	179	8	60	322	68	322	8.53e-17	80.1
MS.gene014425.t1	AT2G29290	26.616	263	173	7	56	311	3	252	1.45e-16	78.6
MS.gene014425.t1	AT3G05260	25.368	272	179	6	50	310	27	285	1.56e-16	79.0
MS.gene014425.t1	AT1G49670	30.769	195	120	4	65	245	9	202	4.18e-16	79.3
MS.gene014425.t1	AT1G49670	30.769	195	120	4	65	245	9	202	4.76e-16	79.3
MS.gene014425.t1	AT4G13180	26.718	262	172	7	58	310	11	261	1.22e-15	75.9
MS.gene014425.t1	AT1G07440	26.172	256	174	6	60	311	12	256	1.35e-15	75.9
MS.gene014425.t1	AT3G12800	28.986	276	166	9	60	319	10	271	1.63e-15	76.3
MS.gene014425.t1	AT2G29340	27.041	196	137	3	56	247	3	196	7.22e-15	72.8
MS.gene014425.t1	AT2G29340	27.041	196	137	3	56	247	3	196	7.22e-15	72.8
MS.gene014425.t1	AT2G29310	26.957	230	154	6	86	311	31	250	1.27e-14	73.2
MS.gene014425.t1	AT5G04900	27.461	193	131	3	66	249	83	275	8.98e-14	71.6
MS.gene014425.t1	AT2G29310	27.551	196	136	3	56	247	3	196	1.39e-13	68.9
MS.gene014425.t1	AT3G47350	27.861	201	132	6	63	256	47	241	1.80e-13	70.5
MS.gene014425.t1	AT3G47350	27.861	201	132	6	63	256	47	241	2.13e-13	70.1
MS.gene014425.t1	AT2G29350	26.754	228	149	6	94	316	16	230	1.16e-12	67.0
MS.gene014425.t1	AT5G65205	28.495	186	124	4	61	243	7	186	1.47e-12	67.4
MS.gene014425.t1	AT1G10310	28.351	194	123	4	63	248	18	203	1.70e-12	66.6
MS.gene014425.t1	AT2G29320	26.531	196	137	4	55	245	8	201	1.37e-11	63.5
MS.gene014425.t1	AT2G29290	23.423	222	153	5	93	311	28	235	8.72e-11	61.6

Find 68 sgRNAs with CRISPR-Local

Find 106 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GTTCACAATTCGCTCCTTTC+AGG	0.161977	3.1:-90241048	None:intergenic
GACAAGGTAGCACTCATAAC+TGG	0.256050	3.1:+90240413	MS.gene014425:CDS
GTATCCAAGTTTGCGGTTAT+AGG	0.264110	3.1:+90240863	MS.gene014425:CDS
GCAAGAAAATTCATCAATAA+TGG	0.327600	3.1:+90240464	MS.gene014425:CDS
TCTTTCTAATGCACTTAATC+TGG	0.330602	3.1:-90241190	None:intergenic
TTATAGGGATGCAAGGAAAA+AGG	0.362837	3.1:+90239760	MS.gene014425:intron
GTGTGAGATCTGATTCATTT+TGG	0.372785	3.1:+90239813	MS.gene014425:CDS
GTAATGATCCCGCGTGGAAC+TGG	0.374917	3.1:+90240776	MS.gene014425:CDS
AATAAATGTTTGTGGCGTTA+TGG	0.400168	3.1:+90240730	MS.gene014425:CDS
CAAGTTATGAAGGTAGCATT+AGG	0.406451	3.1:-90240551	None:intergenic
AGATCGAGGTCAATAACGTT+TGG	0.408258	3.1:-90240683	None:intergenic
TGCAGCATTAGCAATATCAT+TGG	0.411118	3.1:-90241071	None:intergenic
TTTCCTGGCCAAGTTGTTGT+TGG	0.418117	3.1:-90240511	None:intergenic
TGAATAGAATAAAATGCAAA+AGG	0.422613	3.1:-90239535	None:intergenic
ATTGACTCGGATTTCATGCC+TGG	0.424967	3.1:-90240915	None:intergenic
GTAGAAATTGTTCATAATGT+TGG	0.425856	3.1:+90241022	MS.gene014425:CDS
CTATGCACTGCCAGTGTCAC+TGG	0.430322	3.1:+90240806	MS.gene014425:CDS
AATGCCTATAACCGCAAACT+TGG	0.438642	3.1:-90240867	None:intergenic
CCATGGCTTCAGAGCTATCC+AGG	0.442092	3.1:+90240897	MS.gene014425:CDS
GCCAGTGTCACTGGTGTGAT+TGG	0.442709	3.1:+90240815	MS.gene014425:CDS
GTATATCAGCGGGCATACTT+TGG	0.458992	3.1:+90241120	MS.gene014425:CDS
GATATCATGTATAACAATGC+AGG	0.475659	3.1:+90240641	MS.gene014425:CDS
TAATGCACTTAATCTGGTGC+AGG	0.475984	3.1:-90241184	None:intergenic
ATACTCTGTATCCAAGTTTG+CGG	0.478404	3.1:+90240856	MS.gene014425:CDS
GTCATGAAAATAAATGTTTG+TGG	0.480828	3.1:+90240722	MS.gene014425:CDS
TATAGGCATTGTTAAATCCA+TGG	0.486525	3.1:+90240880	MS.gene014425:CDS
GAATAGAATAAAATGCAAAA+GGG	0.490448	3.1:-90239534	None:intergenic
AAGTTGTTTGTATTCAGAAA+CGG	0.501899	3.1:-90240618	None:intergenic
TCCATTTCGCACATGACAAA+AGG	0.506027	3.1:-90240965	None:intergenic
GCAGCATTAGCAATATCATT+GGG	0.508284	3.1:-90241070	None:intergenic
AATGTTTGTGGCGTTATGGC+TGG	0.510119	3.1:+90240734	MS.gene014425:CDS
TGTGACGTTGCAAGTTATGA+AGG	0.510852	3.1:-90240561	None:intergenic
CAGGAAACTGCAAAAGAACT+AGG	0.510876	3.1:+90240527	MS.gene014425:CDS
ACCTTGCATCTAATGATGCT+AGG	0.517372	3.1:+90241098	MS.gene014425:CDS
GACACTGGCAGTGCATAGAA+TGG	0.519009	3.1:-90240801	None:intergenic
CCTGGATAGCTCTGAAGCCA+TGG	0.523976	3.1:-90240897	None:intergenic
CATACTTTGGCTGTGGATGG+AGG	0.528711	3.1:+90241133	MS.gene014425:CDS
ACAAGGTAGCACTCATAACT+GGG	0.533686	3.1:+90240414	MS.gene014425:CDS
AGTGTCACTGGTGTGATTGG+AGG	0.535327	3.1:+90240818	MS.gene014425:CDS
CACTGGTGTGATTGGAGGGA+TGG	0.539300	3.1:+90240823	MS.gene014425:CDS
CAGCGGGCATACTTTGGCTG+TGG	0.539481	3.1:+90241126	MS.gene014425:CDS
TCCAATCACACCAGTGACAC+TGG	0.545725	3.1:-90240816	None:intergenic
GTGTCACTGGTGTGATTGGA+GGG	0.547063	3.1:+90240819	MS.gene014425:CDS
ATTTGTCTTATAGGGATGCA+AGG	0.549438	3.1:+90239753	MS.gene014425:intron
GCTGATATCCAACAACAACT+TGG	0.550507	3.1:+90240503	MS.gene014425:CDS
GGGCATACTTTGGCTGTGGA+TGG	0.556081	3.1:+90241130	MS.gene014425:CDS
TCGAGGTCAATAACGTTTGG+AGG	0.558071	3.1:-90240680	None:intergenic
TGAAGGTTTGCATCGAGCAA+AGG	0.561575	3.1:+90239600	MS.gene014425:CDS
TATCCAACAACAACTTGGCC+AGG	0.561816	3.1:+90240508	MS.gene014425:CDS
ACCTAGCATCATTAGATGCA+AGG	0.564196	3.1:-90241099	None:intergenic
CATAATGTTGGAGTCCTGAA+AGG	0.573360	3.1:+90241034	MS.gene014425:CDS
AAAGGAGTAGGAATAGCAAA+AGG	0.575525	3.1:-90240947	None:intergenic
CTTCTCACAGTATCAAGTGA+AGG	0.582485	3.1:+90239583	MS.gene014425:CDS
ATATCATGTATAACAATGCA+GGG	0.592185	3.1:+90240642	MS.gene014425:CDS
AATAGAATAAAATGCAAAAG+GGG	0.599200	3.1:-90239533	None:intergenic
TCGCACATGACAAAAGGAGT+AGG	0.606020	3.1:-90240959	None:intergenic
ATGAATCAGATCTCACACAC+TGG	0.636749	3.1:-90239808	None:intergenic
GTCAAGGAAGCTACAAGACA+AGG	0.659741	3.1:+90240397	MS.gene014425:intron
ATGATGCTAGGTATATCAGC+GGG	0.660569	3.1:+90241110	MS.gene014425:CDS
AAGGCGATATACAATTGACT+CGG	0.673213	3.1:-90240928	None:intergenic
AGTCGCTGCGAATCAAGATG+TGG	0.674277	3.1:-90240998	None:intergenic
GAATGGATCCAGTTCCACGC+GGG	0.678631	3.1:-90240784	None:intergenic
TGTCAAACGATTCTAGATCG+AGG	0.680208	3.1:-90240697	None:intergenic
CAAGGTAGCACTCATAACTG+GGG	0.699303	3.1:+90240415	MS.gene014425:CDS
AAGGTAGCACTCATAACTGG+GGG	0.699422	3.1:+90240416	MS.gene014425:CDS
AATGATGCTAGGTATATCAG+CGG	0.703024	3.1:+90241109	MS.gene014425:CDS
GCGCGTGTAATGATCCCGCG+TGG	0.726381	3.1:+90240770	MS.gene014425:CDS
AGAATGGATCCAGTTCCACG+CGG	0.782409	3.1:-90240785	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATTAAGTAAATATTATCAAA+AGG	+	chr3.1:90240325-90240344	MS.gene014425:intron	10.0%
!!!	TAATTTTTATTTGTCTTATA+GGG	+	chr3.1:90239745-90239764	MS.gene014425:intron	10.0%
!!	AGTAAATTTAAATAGAGAAA+AGG	-	chr3.1:90239877-90239896	None:intergenic	15.0%
!!!	CTAATTTTTATTTGTCTTAT+AGG	+	chr3.1:90239744-90239763	MS.gene014425:intron	15.0%
!!	AAAAAAAAAGTGACTTTCAA+TGG	-	chr3.1:90239719-90239738	None:intergenic	20.0%
!!	AATAGAATAAAATGCAAAAG+GGG	-	chr3.1:90239536-90239555	None:intergenic	20.0%
!!	GAATAGAATAAAATGCAAAA+GGG	-	chr3.1:90239537-90239556	None:intergenic	20.0%
!!	TGAATAGAATAAAATGCAAA+AGG	-	chr3.1:90239538-90239557	None:intergenic	20.0%
!!!	TTTCACATCTTTTAAGAATT+TGG	+	chr3.1:90241156-90241175	MS.gene014425:CDS	20.0%
!	AAACTAAAGACAAAAACTGA+AGG	-	chr3.1:90240134-90240153	None:intergenic	25.0%
!	AAGTTGTTTGTATTCAGAAA+CGG	-	chr3.1:90240621-90240640	None:intergenic	25.0%
!	ATATCATGTATAACAATGCA+GGG	+	chr3.1:90240642-90240661	MS.gene014425:CDS	25.0%
!	ATCCTATGGATAAATAGAAA+AGG	-	chr3.1:90240164-90240183	None:intergenic	25.0%
!	ATGACTAAGAGATTATATCA+TGG	-	chr3.1:90240240-90240259	None:intergenic	25.0%
!	GATATTCAATAGTAGATAAC+TGG	-	chr3.1:90239970-90239989	None:intergenic	25.0%
!	GCAAGAAAATTCATCAATAA+TGG	+	chr3.1:90240464-90240483	MS.gene014425:CDS	25.0%
!	GTAGAAATTGTTCATAATGT+TGG	+	chr3.1:90241022-90241041	MS.gene014425:CDS	25.0%
!	GTCATGAAAATAAATGTTTG+TGG	+	chr3.1:90240722-90240741	MS.gene014425:CDS	25.0%
!	GTTAAGGAATATATATGCAT+AGG	-	chr3.1:90239653-90239672	None:intergenic	25.0%
!	TATAGAAAAAGATACTGCAA+GGG	-	chr3.1:90239692-90239711	None:intergenic	25.0%
!	TATTCTGATAAAAACTTGAC+TGG	+	chr3.1:90240198-90240217	MS.gene014425:intron	25.0%
!	TTATAGAAAAAGATACTGCA+AGG	-	chr3.1:90239693-90239712	None:intergenic	25.0%
!!	AGTGTTAATTTGGTTAATCT+TGG	+	chr3.1:90240003-90240022	MS.gene014425:intron	25.0%
!!	TTCCTTTTCTATTTATCCAT+AGG	+	chr3.1:90240159-90240178	MS.gene014425:intron	25.0%
!!	TTTTCTATTTATCCATAGGA+TGG	+	chr3.1:90240163-90240182	MS.gene014425:intron	25.0%
	AATAAATGTTTGTGGCGTTA+TGG	+	chr3.1:90240730-90240749	MS.gene014425:CDS	30.0%
	CAACTTCTGTAGTGTTAATT+TGG	+	chr3.1:90239993-90240012	MS.gene014425:intron	30.0%
	GATATCATGTATAACAATGC+AGG	+	chr3.1:90240641-90240660	MS.gene014425:CDS	30.0%
!	AAGGTGAAAGCATAAAAATG+AGG	-	chr3.1:90239858-90239877	None:intergenic	30.0%
!	CTTTTCATTTACGTTTGATG+TGG	+	chr3.1:90240372-90240391	MS.gene014425:intron	30.0%
!	TATAGGCATTGTTAAATCCA+TGG	+	chr3.1:90240880-90240899	MS.gene014425:CDS	30.0%
	AAAGGAGTAGGAATAGCAAA+AGG	-	chr3.1:90240950-90240969	None:intergenic	35.0%
	AAGGCGATATACAATTGACT+CGG	-	chr3.1:90240931-90240950	None:intergenic	35.0%
	AATAACAGTTTGCCATCCTA+TGG	-	chr3.1:90240178-90240197	None:intergenic	35.0%
	AATGATGCTAGGTATATCAG+CGG	+	chr3.1:90241109-90241128	MS.gene014425:CDS	35.0%
	ATACTCTGTATCCAAGTTTG+CGG	+	chr3.1:90240856-90240875	MS.gene014425:CDS	35.0%
	ATTTGTCTTATAGGGATGCA+AGG	+	chr3.1:90239753-90239772	MS.gene014425:intron	35.0%
	CAAGTTATGAAGGTAGCATT+AGG	-	chr3.1:90240554-90240573	None:intergenic	35.0%
	GCAGCATTAGCAATATCATT+GGG	-	chr3.1:90241073-90241092	None:intergenic	35.0%
	TATTGTCTACTCAGACTGTA+AGG	+	chr3.1:90240094-90240113	MS.gene014425:intron	35.0%
	TGCAGCATTAGCAATATCAT+TGG	-	chr3.1:90241074-90241093	None:intergenic	35.0%
	TTAGATTAATCAAACGACCG+AGG	+	chr3.1:90240259-90240278	MS.gene014425:intron	35.0%
	TTATAGGGATGCAAGGAAAA+AGG	+	chr3.1:90239760-90239779	MS.gene014425:intron	35.0%
	TTCATTTACGTTTGATGTGG+TGG	+	chr3.1:90240375-90240394	MS.gene014425:intron	35.0%
!	ACAAAAACTGAAGGCTTGTT+AGG	-	chr3.1:90240125-90240144	None:intergenic	35.0%
!	GTGTGAGATCTGATTCATTT+TGG	+	chr3.1:90239813-90239832	MS.gene014425:CDS	35.0%
!!	TAATTTGCTCGATGTGGTTA+AGG	-	chr3.1:90239669-90239688	None:intergenic	35.0%
	AATGCCTATAACCGCAAACT+TGG	-	chr3.1:90240870-90240889	None:intergenic	40.0%
	ACAAGGTAGCACTCATAACT+GGG	+	chr3.1:90240414-90240433	MS.gene014425:CDS	40.0%
	ACCTAGCATCATTAGATGCA+AGG	-	chr3.1:90241102-90241121	None:intergenic	40.0%
	ACCTTGCATCTAATGATGCT+AGG	+	chr3.1:90241098-90241117	MS.gene014425:CDS	40.0%
	AGATCGAGGTCAATAACGTT+TGG	-	chr3.1:90240686-90240705	None:intergenic	40.0%
	ATCATCCTCTGTGGTTTCTT+TGG	-	chr3.1:90240290-90240309	None:intergenic	40.0%
	ATGAATCAGATCTCACACAC+TGG	-	chr3.1:90239811-90239830	None:intergenic	40.0%
	ATGATGCTAGGTATATCAGC+GGG	+	chr3.1:90241110-90241129	MS.gene014425:CDS	40.0%
	CAGGAAACTGCAAAAGAACT+AGG	+	chr3.1:90240527-90240546	MS.gene014425:CDS	40.0%
	CATAATGTTGGAGTCCTGAA+AGG	+	chr3.1:90241034-90241053	MS.gene014425:CDS	40.0%
	CATCATTTGATCATCCTCTG+TGG	-	chr3.1:90240299-90240318	None:intergenic	40.0%
	CTTCTCACAGTATCAAGTGA+AGG	+	chr3.1:90239583-90239602	MS.gene014425:CDS	40.0%
	GCTGATATCCAACAACAACT+TGG	+	chr3.1:90240503-90240522	MS.gene014425:CDS	40.0%
	GTATCCAAGTTTGCGGTTAT+AGG	+	chr3.1:90240863-90240882	MS.gene014425:CDS	40.0%
	TCCATTTCGCACATGACAAA+AGG	-	chr3.1:90240968-90240987	None:intergenic	40.0%
	TCTACTCAGACTGTAAGGTA+TGG	+	chr3.1:90240099-90240118	MS.gene014425:intron	40.0%
	TGGTTTCTTTGGTCTAACCT+CGG	-	chr3.1:90240279-90240298	None:intergenic	40.0%
	TGTCAAACGATTCTAGATCG+AGG	-	chr3.1:90240700-90240719	None:intergenic	40.0%
	TGTGACGTTGCAAGTTATGA+AGG	-	chr3.1:90240564-90240583	None:intergenic	40.0%
	TTAGACCAAAGAAACCACAG+AGG	+	chr3.1:90240282-90240301	MS.gene014425:intron	40.0%
!	TCCTTTTGTCATGTGCGAAA+TGG	+	chr3.1:90240964-90240983	MS.gene014425:CDS	40.0%
!!	CTAGTTCTTTTGCAGTTTCC+TGG	-	chr3.1:90240529-90240548	None:intergenic	40.0%
	AAGGTAGCACTCATAACTGG+GGG	+	chr3.1:90240416-90240435	MS.gene014425:CDS	45.0%
	AATGTTTGTGGCGTTATGGC+TGG	+	chr3.1:90240734-90240753	MS.gene014425:CDS	45.0%
	CAAGGTAGCACTCATAACTG+GGG	+	chr3.1:90240415-90240434	MS.gene014425:CDS	45.0%
	GACAAGGTAGCACTCATAAC+TGG	+	chr3.1:90240413-90240432	MS.gene014425:CDS	45.0%
	GATGCAAGGAAAAAGGTCAG+AGG	+	chr3.1:90239767-90239786	MS.gene014425:CDS	45.0%
	GTATATCAGCGGGCATACTT+TGG	+	chr3.1:90241120-90241139	MS.gene014425:CDS	45.0%
	GTCAAGGAAGCTACAAGACA+AGG	+	chr3.1:90240397-90240416	MS.gene014425:intron	45.0%
	GTTCACAATTCGCTCCTTTC+AGG	-	chr3.1:90241051-90241070	None:intergenic	45.0%
	TACGTTTGATGTGGTGGTCA+AGG	+	chr3.1:90240381-90240400	MS.gene014425:intron	45.0%
	TATCCAACAACAACTTGGCC+AGG	+	chr3.1:90240508-90240527	MS.gene014425:CDS	45.0%
	TCGAGGTCAATAACGTTTGG+AGG	-	chr3.1:90240683-90240702	None:intergenic	45.0%
	TCGCACATGACAAAAGGAGT+AGG	-	chr3.1:90240962-90240981	None:intergenic	45.0%
	TGAAGGTTTGCATCGAGCAA+AGG	+	chr3.1:90239600-90239619	MS.gene014425:CDS	45.0%
	TTTCCTGGCCAAGTTGTTGT+TGG	-	chr3.1:90240514-90240533	None:intergenic	45.0%
!	AACGTTTGGAGGAGTTTTGC+AGG	-	chr3.1:90240672-90240691	None:intergenic	45.0%
!	ACGTTTGGAGGAGTTTTGCA+GGG	-	chr3.1:90240671-90240690	None:intergenic	45.0%
!!	ATTGACTCGGATTTCATGCC+TGG	-	chr3.1:90240918-90240937	None:intergenic	45.0%
!!	CAAGGGTAATTTGCTCGATG+TGG	-	chr3.1:90239675-90239694	None:intergenic	45.0%
	AGAATGGATCCAGTTCCACG+CGG	-	chr3.1:90240788-90240807	None:intergenic	50.0%
	AGTCGCTGCGAATCAAGATG+TGG	-	chr3.1:90241001-90241020	None:intergenic	50.0%
	CATACTTTGGCTGTGGATGG+AGG	+	chr3.1:90241133-90241152	MS.gene014425:CDS	50.0%
	GACACTGGCAGTGCATAGAA+TGG	-	chr3.1:90240804-90240823	None:intergenic	50.0%
	TCCAATCACACCAGTGACAC+TGG	-	chr3.1:90240819-90240838	None:intergenic	50.0%
!	AGTGTCACTGGTGTGATTGG+AGG	+	chr3.1:90240818-90240837	MS.gene014425:CDS	50.0%
!	GTGTCACTGGTGTGATTGGA+GGG	+	chr3.1:90240819-90240838	MS.gene014425:CDS	50.0%
	ACTGGGGGAGCTAGTGGAAT+AGG	+	chr3.1:90240431-90240450	MS.gene014425:CDS	55.0%
	CACTGGTGTGATTGGAGGGA+TGG	+	chr3.1:90240823-90240842	MS.gene014425:CDS	55.0%
	CCATGGCTTCAGAGCTATCC+AGG	+	chr3.1:90240897-90240916	MS.gene014425:CDS	55.0%
	CTATGCACTGCCAGTGTCAC+TGG	+	chr3.1:90240806-90240825	MS.gene014425:CDS	55.0%
	CTCATAACTGGGGGAGCTAG+TGG	+	chr3.1:90240425-90240444	MS.gene014425:CDS	55.0%
	GAATGGATCCAGTTCCACGC+GGG	-	chr3.1:90240787-90240806	None:intergenic	55.0%
	GGGCATACTTTGGCTGTGGA+TGG	+	chr3.1:90241130-90241149	MS.gene014425:CDS	55.0%
	GTAATGATCCCGCGTGGAAC+TGG	+	chr3.1:90240776-90240795	MS.gene014425:CDS	55.0%
!	GCCAGTGTCACTGGTGTGAT+TGG	+	chr3.1:90240815-90240834	MS.gene014425:CDS	55.0%
!!	CCTGGATAGCTCTGAAGCCA+TGG	-	chr3.1:90240900-90240919	None:intergenic	55.0%
	CAGCGGGCATACTTTGGCTG+TGG	+	chr3.1:90241126-90241145	MS.gene014425:CDS	60.0%
	GCGCGTGTAATGATCCCGCG+TGG	+	chr3.1:90240770-90240789	MS.gene014425:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.1	gene	90239530	90241198	90239530	ID=MS.gene014425
chr3.1	mRNA	90239530	90241198	90239530	ID=MS.gene014425.t1;Parent=MS.gene014425
chr3.1	exon	90239530	90239621	90239530	ID=MS.gene014425.t1.exon1;Parent=MS.gene014425.t1
chr3.1	CDS	90239530	90239621	90239530	ID=cds.MS.gene014425.t1;Parent=MS.gene014425.t1
chr3.1	exon	90239766	90239846	90239766	ID=MS.gene014425.t1.exon2;Parent=MS.gene014425.t1
chr3.1	CDS	90239766	90239846	90239766	ID=cds.MS.gene014425.t1;Parent=MS.gene014425.t1
chr3.1	exon	90240403	90241198	90240403	ID=MS.gene014425.t1.exon3;Parent=MS.gene014425.t1
chr3.1	CDS	90240403	90241198	90240403	ID=cds.MS.gene014425.t1;Parent=MS.gene014425.t1

Gene Sequence

>MS.gene014425

ATGCCCCTTTTGCATTTTATTCTATTCATTTTTATTTCACACTGCATAAAGCTCTTCTCACAGTATCAAGTGAAGGTTTGCATCGAGCAAAGGTTTTTGTTTGATAACAAAATTATTACTCCTATGCATATATATTCCTTAACCACATCGAGCAAATTACCCTTGCAGTATCTTTTTCTATAACAACCATTGAAAGTCACTTTTTTTTTTCTCTCTAATTTTTATTTGTCTTATAGGGATGCAAGGAAAAAGGTCAGAGGCTCAAACTACATTACACTCCAGTGTGTGAGATCTGATTCATTTTGGCAGAAAATCATGTTTTACACCTCATTTTTATGCTTTCACCTTTTCTCTATTTAAATTTACTTTCTCAGCTATCTATACTTTCAGTTGATGATTAAAAAAAATTAAGTATAAGATGTTCTGTTTGTATTTTTCCAGTTATCTACTATTGAATATCTTTCAACTTCTGTAGTGTTAATTTGGTTAATCTTGGATGCATATATGAAGTTTTGTGAATATGTTTTCAGCTTACAGTGTTTCTGTTAATTATTAAAAACAGATTATTGTCTACTCAGACTGTAAGGTATGGCCTAACAAGCCTTCAGTTTTTGTCTTTAGTTTACATTTTCCTTTTCTATTTATCCATAGGATGGCAAACTGTTATTTATTCTGATAAAAACTTGACTGGCTGATTGATTTAACGACCATGATATAATCTCTTAGTCATTAGATTAATCAAACGACCGAGGTTAGACCAAAGAAACCACAGAGGATGATCAAATGATGCTCAACATTAAGTAAATATTATCAAAAGGATCTTTGTTGTTTATTGTTAAATGCTTTTCATTTACGTTTGATGTGGTGGTCAAGGAAGCTACAAGACAAGGTAGCACTCATAACTGGGGGAGCTAGTGGAATAGGAAAAGCTGCAGCAAGAAAATTCATCAATAATGGAGCAAAAGTAATTATTGCTGATATCCAACAACAACTTGGCCAGGAAACTGCAAAAGAACTAGGACCTAATGCTACCTTCATAACTTGCAACGTCACAAAAGAATCAGACATATCAGATGCAGTTGATTTCGCCGTTTCTGAATACAAACAACTTGATATCATGTATAACAATGCAGGGATACCCTGCAAAACTCCTCCAAACGTTATTGACCTCGATCTAGAATCGTTTGACAAAGTCATGAAAATAAATGTTTGTGGCGTTATGGCTGGCATCAAGCATGCAGCGCGTGTAATGATCCCGCGTGGAACTGGATCCATTCTATGCACTGCCAGTGTCACTGGTGTGATTGGAGGGATGGCGCAGCATACATACTCTGTATCCAAGTTTGCGGTTATAGGCATTGTTAAATCCATGGCTTCAGAGCTATCCAGGCATGAAATCCGAGTCAATTGTATATCGCCTTTTGCTATTCCTACTCCTTTTGTCATGTGCGAAATGGATCAGATATATCCACATCTTGATTCGCAGCGACTTGTAGAAATTGTTCATAATGTTGGAGTCCTGAAAGGAGCGAATTGTGAACCCAATGATATTGCTAATGCTGCACTTTACCTTGCATCTAATGATGCTAGGTATATCAGCGGGCATACTTTGGCTGTGGATGGAGGTTTCACATCTTTTAAGAATTTGGAGTTTCCTGCACCAGATTAA

Protein sequence

>MS.gene014425.t1

MPLLHFILFIFISHCIKLFSQYQVKVCIEQRDARKKVRGSNYITLQCVRSDSFWQKIMKLQDKVALITGGASGIGKAAARKFINNGAKVIIADIQQQLGQETAKELGPNATFITCNVTKESDISDAVDFAVSEYKQLDIMYNNAGIPCKTPPNVIDLDLESFDKVMKINVCGVMAGIKHAARVMIPRGTGSILCTASVTGVIGGMAQHTYSVSKFAVIGIVKSMASELSRHEIRVNCISPFAIPTPFVMCEMDQIYPHLDSQRLVEIVHNVGVLKGANCEPNDIANAALYLASNDARYISGHTLAVDGGFTSFKNLEFPAPD