AlfalfaGEDB | MS.gene50522

M. sativa cultivar XinJiangDaYe / MS.gene50522

KEGG: K00059
GO: GO:0003674 GO:0003824 GO:0016491
PFAM: PF13561.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50522.t1	XP_013449698.1	96.5	255	9	0	1	255	1	255	7.90E-123	449.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50522.t1	Q9SQR4	67.2	259	78	1	4	255	12	270	8.3e-89	328.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50522.t1	A0A072U2V3	96.5	255	9	0	1	255	1	255	5.7e-123	449.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene003926	MS.gene50522	PPI
MS.gene63484	MS.gene50522	PPI
MS.gene012681	MS.gene50522	PPI
MS.gene50522	MS.gene03319	PPI
MS.gene50522	MS.gene63482	PPI
MS.gene50522	MS.gene043387	PPI
MS.gene021164	MS.gene50522	PPI
MS.gene50522	MS.gene014425	PPI
MS.gene50522	MS.gene68880	PPI
MS.gene50522	MS.gene08695	PPI
MS.gene50522	MS.gene042142	PPI
MS.gene50522	MS.gene008161	PPI
MS.gene63483	MS.gene50522	PPI
MS.gene50522	MS.gene006999	PPI
MS.gene42856	MS.gene50522	PPI
MS.gene50522	MS.gene42114	PPI
MS.gene050431	MS.gene50522	PPI
MS.gene63403	MS.gene50522	PPI
MS.gene019786	MS.gene50522	PPI
MS.gene85724	MS.gene50522	PPI
MS.gene41122	MS.gene50522	PPI
MS.gene50522	MS.gene25667	PPI
MS.gene50522	MS.gene30551	PPI
MS.gene63306	MS.gene50522	PPI
MS.gene62187	MS.gene50522	PPI
MS.gene50522	MS.gene23019	PPI
MS.gene50522	MS.gene055846	PPI
MS.gene63308	MS.gene50522	PPI
MS.gene033685	MS.gene50522	PPI
MS.gene50522	MS.gene63300	PPI
MS.gene50522	MS.gene25674	PPI
MS.gene50522	MS.gene85724	PPI
MS.gene50522	MS.gene87780	PPI
MS.gene50522	MS.gene008153	PPI
MS.gene50522	MS.gene80360	PPI
MS.gene50522	MS.gene008160	PPI
MS.gene045581	MS.gene50522	PPI
MS.gene50522	MS.gene08756	PPI
MS.gene50522	MS.gene024269	PPI
MS.gene50522	MS.gene003729	PPI
MS.gene28848	MS.gene50522	PPI
MS.gene87026	MS.gene50522	PPI
MS.gene68956	MS.gene50522	PPI
MS.gene50522	MS.gene050131	PPI
MS.gene50522	MS.gene007678	PPI
MS.gene003925	MS.gene50522	PPI
MS.gene62193	MS.gene50522	PPI
MS.gene50522	MS.gene028938	PPI
MS.gene50522	MS.gene003992	PPI
MS.gene073684	MS.gene50522	PPI
MS.gene50522	MS.gene62195	PPI
MS.gene056339	MS.gene50522	PPI
MS.gene07096	MS.gene50522	PPI
MS.gene54952	MS.gene50522	PPI
MS.gene031693	MS.gene50522	PPI
MS.gene050432	MS.gene50522	PPI
MS.gene012794	MS.gene50522	PPI
MS.gene50522	MS.gene73824	PPI
MS.gene007006	MS.gene50522	PPI
MS.gene50522	MS.gene019786	PPI
MS.gene50522	MS.gene007002	PPI
MS.gene28847	MS.gene50522	PPI
MS.gene62194	MS.gene50522	PPI
MS.gene50522	MS.gene43481	PPI
MS.gene63307	MS.gene50522	PPI
MS.gene006617	MS.gene50522	PPI
MS.gene50522	MS.gene54598	PPI
MS.gene008162	MS.gene50522	PPI
MS.gene50522	MS.gene072664	PPI
MS.gene50522	MS.gene07097	PPI
MS.gene50522	MS.gene31507	PPI
MS.gene50522	MS.gene28847	PPI
MS.gene50522	MS.gene95020	PPI
MS.gene50522	MS.gene065380	PPI
MS.gene42260	MS.gene50522	PPI
MS.gene08694	MS.gene50522	PPI
MS.gene064756	MS.gene50522	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50522.t1	MTR_7g094510	96.471	255	9	0	1	255	1	255	0.0	499
MS.gene50522.t1	MTR_7g094520	83.004	253	43	0	3	255	8	260	2.57e-154	430
MS.gene50522.t1	MTR_1g068955	69.600	250	75	1	4	252	12	261	1.96e-120	345
MS.gene50522.t1	MTR_3g085740	35.628	247	152	5	10	255	79	319	6.58e-37	132
MS.gene50522.t1	MTR_3g068070	33.333	258	150	10	6	254	15	259	4.05e-31	116
MS.gene50522.t1	MTR_3g068085	32.432	259	151	10	6	254	15	259	3.99e-30	114
MS.gene50522.t1	MTR_4g064887	34.118	255	150	9	6	253	15	258	2.78e-29	111
MS.gene50522.t1	MTR_3g068065	33.721	258	149	10	6	254	17	261	5.99e-29	110
MS.gene50522.t1	MTR_3g068175	32.819	259	148	11	6	253	17	260	7.32e-28	107
MS.gene50522.t1	MTR_6g023610	31.008	258	166	5	4	255	11	262	4.77e-27	105
MS.gene50522.t1	MTR_7g063260	31.660	259	157	7	6	254	16	264	6.54e-27	105
MS.gene50522.t1	MTR_7g063320	31.008	258	162	7	6	255	15	264	1.08e-26	104
MS.gene50522.t1	MTR_6g023590	30.798	263	160	7	3	254	10	261	1.57e-26	104
MS.gene50522.t1	MTR_7g071680	32.967	273	149	10	6	253	29	292	1.86e-26	104
MS.gene50522.t1	MTR_7g063270	31.008	258	159	7	6	254	16	263	2.49e-26	103
MS.gene50522.t1	MTR_6g023910	31.154	260	167	6	2	255	9	262	6.67e-26	102
MS.gene50522.t1	MTR_6g023600	30.116	259	167	6	3	254	10	261	9.76e-26	102
MS.gene50522.t1	MTR_3g068175	32.319	263	148	12	6	253	17	264	1.11e-25	102
MS.gene50522.t1	MTR_6g081270	72.308	65	18	0	28	92	29	93	1.98e-25	96.7
MS.gene50522.t1	MTR_7g063360	31.250	256	162	6	6	254	16	264	2.04e-25	101
MS.gene50522.t1	MTR_7g063390	30.078	256	165	6	6	254	16	264	2.54e-25	100
MS.gene50522.t1	MTR_6g023600	30.116	259	161	6	3	254	10	255	2.62e-25	100
MS.gene50522.t1	MTR_3g068155	30.315	254	161	9	6	253	19	262	3.60e-25	100
MS.gene50522.t1	MTR_4g064883	31.200	250	164	6	6	253	14	257	5.13e-25	100
MS.gene50522.t1	MTR_1196s0010	32.411	253	152	7	8	255	276	514	2.13e-24	101
MS.gene50522.t1	MTR_6g023990	30.769	260	166	7	2	254	10	262	1.73e-23	96.3
MS.gene50522.t1	MTR_7g107670	29.771	262	160	9	6	254	33	283	3.75e-23	95.9
MS.gene50522.t1	MTR_7g063330	29.249	253	170	4	6	254	15	262	1.74e-22	93.6
MS.gene50522.t1	MTR_3g020670	31.439	264	156	8	6	254	16	269	3.42e-22	92.8
MS.gene50522.t1	MTR_4g131740	28.125	256	174	5	6	255	40	291	4.36e-22	92.8
MS.gene50522.t1	MTR_6g023950	29.771	262	164	7	3	254	10	261	4.40e-22	92.4
MS.gene50522.t1	MTR_7g105580	29.740	269	141	9	1	254	1	236	2.30e-21	90.1
MS.gene50522.t1	MTR_4g132850	30.522	249	164	7	6	253	7	247	7.33e-21	89.0
MS.gene50522.t1	MTR_4g078630	28.846	260	164	5	6	253	19	269	1.69e-20	88.6
MS.gene50522.t1	MTR_7g083870	29.502	261	163	8	6	254	35	286	3.55e-20	87.8
MS.gene50522.t1	MTR_4g058810	30.000	260	161	10	6	253	11	261	5.07e-20	87.0
MS.gene50522.t1	MTR_7g105590	30.348	201	132	3	1	199	1	195	1.53e-19	84.0
MS.gene50522.t1	MTR_8g442750	31.343	268	147	9	6	253	14	264	1.77e-19	85.9
MS.gene50522.t1	MTR_7g113650	27.138	269	159	9	6	255	40	290	2.72e-19	85.1
MS.gene50522.t1	MTR_4g058840	29.615	260	162	10	6	253	11	261	6.97e-19	84.0
MS.gene50522.t1	MTR_4g058820	29.615	260	162	10	6	253	11	261	6.97e-19	84.0
MS.gene50522.t1	MTR_1g082760	29.213	267	156	9	6	255	40	290	3.16e-18	82.4
MS.gene50522.t1	MTR_7g105460	29.845	258	161	9	6	254	13	259	3.42e-18	82.0
MS.gene50522.t1	MTR_1g051165	29.434	265	161	9	6	253	14	269	4.80e-18	81.6
MS.gene50522.t1	MTR_4g064887	34.328	201	115	8	6	200	15	204	1.76e-17	79.0
MS.gene50522.t1	MTR_4g052320	27.953	254	169	7	6	254	8	252	2.21e-17	79.7
MS.gene50522.t1	MTR_4g052350	27.612	268	167	8	1	253	1	256	3.64e-17	79.0
MS.gene50522.t1	MTR_4g052340	26.966	267	168	6	1	252	1	255	8.72e-17	78.2
MS.gene50522.t1	MTR_4g052300	29.231	260	155	9	6	253	8	250	1.12e-16	77.8
MS.gene50522.t1	MTR_7g063270	37.374	99	58	1	160	254	116	214	1.03e-15	73.9
MS.gene50522.t1	MTR_4g064883	31.122	196	128	5	6	200	14	203	2.11e-15	73.2
MS.gene50522.t1	MTR_4g066050	29.208	202	126	7	8	201	26	218	8.18e-15	72.4
MS.gene50522.t1	MTR_4g134660	29.592	196	131	5	8	201	8	198	1.99e-14	70.9
MS.gene50522.t1	MTR_3g113870	28.780	205	134	7	14	216	29	223	1.73e-13	68.2
MS.gene50522.t1	MTR_7g080410	27.320	194	138	3	6	198	38	229	3.29e-13	68.6
MS.gene50522.t1	MTR_7g080410	27.320	194	138	3	6	198	38	229	3.75e-13	68.2
MS.gene50522.t1	MTR_7g110130	26.996	263	173	7	1	254	1	253	1.21e-12	66.2
MS.gene50522.t1	MTR_7g110140	27.027	259	177	7	1	254	1	252	3.56e-12	65.1
MS.gene50522.t1	MTR_7g105570	31.579	171	105	5	92	254	50	216	3.85e-12	64.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50522.t1	AT3G03980	70.656	259	69	1	4	255	12	270	7.03e-130	368
MS.gene50522.t1	AT5G18210	68.775	253	76	1	6	255	8	260	8.19e-126	358
MS.gene50522.t1	AT5G18210	70.339	236	67	1	6	238	8	243	7.93e-117	335
MS.gene50522.t1	AT3G04000	62.016	258	90	1	4	253	12	269	1.16e-115	332
MS.gene50522.t1	AT4G13180	58.268	254	106	0	2	255	9	262	1.76e-110	319
MS.gene50522.t1	AT2G29290	33.200	250	159	6	6	253	7	250	9.71e-32	117
MS.gene50522.t1	AT5G06060	32.411	253	159	8	6	254	9	253	2.04e-31	117
MS.gene50522.t1	AT1G24360	32.653	245	158	5	10	253	78	316	4.89e-31	117
MS.gene50522.t1	AT2G29260	31.837	245	159	6	11	253	73	311	2.05e-28	110
MS.gene50522.t1	AT2G29360	32.794	247	156	6	11	254	21	260	6.63e-28	108
MS.gene50522.t1	AT2G29150	32.283	254	155	7	6	253	16	258	5.61e-27	105
MS.gene50522.t1	AT2G29290	35.979	189	116	5	67	253	48	233	7.46e-27	104
MS.gene50522.t1	AT2G29350	32.389	247	154	7	11	253	20	257	1.42e-26	104
MS.gene50522.t1	AT3G51680	34.962	266	151	10	6	253	32	293	2.20e-25	102
MS.gene50522.t1	AT1G07440	31.102	254	158	8	6	253	12	254	8.18e-25	99.8
MS.gene50522.t1	AT2G30670	32.143	252	159	7	6	253	7	250	1.23e-24	99.4
MS.gene50522.t1	AT3G55310	30.315	254	168	7	6	253	17	267	2.13e-24	99.0
MS.gene50522.t1	AT2G29370	32.411	253	159	7	6	254	16	260	6.55e-24	97.4
MS.gene50522.t1	AT3G46170	29.528	254	170	7	6	253	26	276	1.54e-23	96.7
MS.gene50522.t1	AT1G54870	30.268	261	161	8	6	255	84	334	2.36e-23	97.1
MS.gene50522.t1	AT1G54870	30.268	261	161	8	6	255	82	332	2.47e-23	97.1
MS.gene50522.t1	AT3G55290	29.134	254	171	7	6	253	17	267	4.03e-23	95.5
MS.gene50522.t1	AT3G55290	29.134	254	171	7	6	253	18	268	4.31e-23	95.5
MS.gene50522.t1	AT2G29310	30.588	255	161	8	6	254	7	251	7.87e-23	94.4
MS.gene50522.t1	AT1G52340	28.682	258	168	4	9	254	21	274	1.17e-22	94.4
MS.gene50522.t1	AT3G26760	27.612	268	169	9	2	254	32	289	2.21e-22	94.0
MS.gene50522.t1	AT3G05260	29.297	256	169	6	6	255	37	286	2.26e-22	93.6
MS.gene50522.t1	AT2G29350	33.333	198	122	6	60	253	28	219	2.45e-22	92.4
MS.gene50522.t1	AT1G63380	28.627	255	172	7	6	253	15	266	2.92e-22	93.2
MS.gene50522.t1	AT1G63380	28.627	255	172	7	6	253	17	268	3.11e-22	93.2
MS.gene50522.t1	AT1G63380	28.627	255	172	7	6	253	22	273	3.55e-22	93.2
MS.gene50522.t1	AT1G63380	28.627	255	172	7	6	253	19	270	3.56e-22	93.2
MS.gene50522.t1	AT2G17845	29.231	260	172	8	3	253	44	300	4.25e-22	93.2
MS.gene50522.t1	AT1G62610	30.620	258	163	10	6	253	14	265	1.91e-21	90.9
MS.gene50522.t1	AT1G62610	30.620	258	163	10	6	253	13	264	2.04e-21	90.9
MS.gene50522.t1	AT1G62610	30.620	258	163	10	6	253	15	266	2.08e-21	90.9
MS.gene50522.t1	AT1G62610	30.620	258	163	10	6	253	19	270	2.09e-21	90.9
MS.gene50522.t1	AT1G07450	29.231	260	157	7	6	254	8	251	3.43e-21	89.7
MS.gene50522.t1	AT2G29320	30.709	254	161	7	6	253	13	257	4.30e-21	89.7
MS.gene50522.t1	AT2G29300	30.709	254	161	7	6	253	7	251	6.55e-21	89.4
MS.gene50522.t1	AT2G29300	31.298	262	149	10	6	253	7	251	8.63e-21	89.4
MS.gene50522.t1	AT2G47130	30.385	260	166	7	1	254	1	251	8.65e-21	89.0
MS.gene50522.t1	AT2G29340	31.373	255	157	8	6	253	7	250	1.76e-20	88.2
MS.gene50522.t1	AT2G29340	31.373	255	157	8	6	253	7	250	4.15e-20	87.8
MS.gene50522.t1	AT2G29330	30.233	258	156	9	6	253	7	250	4.41e-20	87.0
MS.gene50522.t1	AT2G47120	29.434	265	161	6	1	254	1	250	4.92e-20	86.7
MS.gene50522.t1	AT3G29250	30.038	263	164	9	1	254	1	252	2.37e-19	85.1
MS.gene50522.t1	AT3G29250	30.038	263	164	9	1	254	37	288	4.70e-19	84.7
MS.gene50522.t1	AT2G47120	29.231	260	158	6	6	254	7	251	1.15e-18	83.2
MS.gene50522.t1	AT2G29310	31.959	194	119	7	67	254	63	249	3.07e-18	82.0
MS.gene50522.t1	AT3G29260	29.278	263	165	8	1	254	1	251	3.12e-18	82.0
MS.gene50522.t1	AT3G29250	29.845	258	161	9	6	254	44	290	7.76e-18	81.3
MS.gene50522.t1	AT4G05530	28.000	250	169	7	6	253	9	249	1.37e-16	77.4
MS.gene50522.t1	AT4G03140	26.154	260	172	5	6	254	78	328	1.46e-16	78.2
MS.gene50522.t1	AT2G29320	31.606	193	120	6	67	253	53	239	1.97e-16	76.6
MS.gene50522.t1	AT3G26770	28.185	259	167	8	6	253	41	291	9.22e-16	75.9
MS.gene50522.t1	AT3G42960	27.376	263	167	7	6	254	8	260	1.20e-15	75.1
MS.gene50522.t1	AT3G12800	29.344	259	165	7	6	253	10	261	1.78e-15	74.7
MS.gene50522.t1	AT2G47140	27.799	259	175	7	1	254	1	252	2.40e-14	71.2
MS.gene50522.t1	AT2G29310	29.592	196	131	5	6	200	7	196	4.21e-13	66.6
MS.gene50522.t1	AT2G29350	30.412	194	128	5	11	203	20	207	5.75e-13	66.6
MS.gene50522.t1	AT5G50600	29.717	212	137	8	6	212	45	249	7.66e-13	67.8
MS.gene50522.t1	AT5G50700	29.717	212	137	8	6	212	45	249	7.66e-13	67.8

Find 80 sgRNAs with CRISPR-Local

Find 91 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGTCAAATTATTCGTGTTAA+TGG	0.232122	7.4:+14710005	MS.gene50522:CDS
GGAAGCGATGACGAAGATTC+TGG	0.310309	7.4:+14709782	MS.gene50522:CDS
ATCATGAACATAAACTTAAA+AGG	0.315108	7.4:+14709624	MS.gene50522:CDS
GCGAAGGAGTTGAAGGGAAC+GGG	0.354344	7.4:+14709804	MS.gene50522:CDS
GATGCTTCTGAATGGGTTAA+TGG	0.360805	7.4:+14709984	MS.gene50522:CDS
AAAGATGTTGCTCCTGTTGT+TGG	0.383642	7.4:+14709948	MS.gene50522:CDS
ACCGGTTCCTCCCGCGGAAT+CGG	0.391420	7.4:+14709312	MS.gene50522:CDS
AGGCGATTTGAAGGCTTCTT+CGG	0.403502	7.4:-14709517	None:intergenic
ACCGATTCCGCGGGAGGAAC+CGG	0.409076	7.4:-14709313	None:intergenic
GTTTGCAATGGTAGGGTACT+TGG	0.410715	7.4:-14709577	None:intergenic
GGCTACCGATGCTTCTGAAT+GGG	0.418333	7.4:+14709977	MS.gene50522:CDS
TGGCTACCGATGCTTCTGAA+TGG	0.420918	7.4:+14709976	MS.gene50522:CDS
ACTGAAGAAGAGAGTCCGTT+TGG	0.431094	7.4:+14709909	MS.gene50522:CDS
CACGGCGTCAAAGGCGGCTG+TGG	0.446425	7.4:+14709761	MS.gene50522:CDS
TGATGGGAAAACGGAGGAGA+TGG	0.454882	7.4:+14709878	MS.gene50522:CDS
GATGTGAACAGGCGATTTGA+AGG	0.457360	7.4:-14709526	None:intergenic
CAAATCGCCTGTTCACATCC+TGG	0.462961	7.4:+14709530	MS.gene50522:CDS
TACTGCGAATTGCGTGGCAC+CGG	0.472414	7.4:+14709830	MS.gene50522:CDS
GAGAGTCCGTTTGGAAGACT+TGG	0.472908	7.4:+14709918	MS.gene50522:CDS
CGTTTGAAGCGAGGCGGAGG+AGG	0.473206	7.4:+14709678	MS.gene50522:CDS
CGCCGAGTGAAGCAAGGTGG+AGG	0.477214	7.4:-14709347	None:intergenic
ATCTCCGTCGCAATCGGTCC+CGG	0.484488	7.4:-14709849	None:intergenic
GATTTACATGGGAGCTTACA+CGG	0.486007	7.4:+14709743	MS.gene50522:CDS
GATTCTGGCGAAGGAGTTGA+AGG	0.492095	7.4:+14709797	MS.gene50522:CDS
CTCTTTACCGATTCCGCGGG+AGG	0.494710	7.4:-14709319	None:intergenic
GAGTTAACCAGGATGTGAAC+AGG	0.500872	7.4:-14709537	None:intergenic
TGATGGCTCGTAAAGTGGTT+TGG	0.501317	7.4:-14709440	None:intergenic
ATCAGCATGGTTGGAGTTGG+AGG	0.501568	7.4:-14709391	None:intergenic
GGTTTGGTTGGTGTTGATCT+CGG	0.503221	7.4:-14709424	None:intergenic
GGAATCAGCATGGTTGGAGT+TGG	0.508679	7.4:-14709394	None:intergenic
CACATCCTGGTTAACTCAGC+CGG	0.511760	7.4:+14709543	MS.gene50522:CDS
ACGGTTGTGTTTGCAATGGT+AGG	0.522093	7.4:-14709585	None:intergenic
GCCCTCCACCTTGCTTCACT+CGG	0.524186	7.4:+14709345	MS.gene50522:CDS
CACGTCAGCGCGGACGGTGA+TGG	0.524209	7.4:-14709457	None:intergenic
GGGAGCTTACACGGCGTCAA+AGG	0.527641	7.4:+14709752	MS.gene50522:CDS
GGCTCGTAAAGTGGTTTGGT+TGG	0.532346	7.4:-14709436	None:intergenic
GGACGCCGGCTGAGTTAACC+AGG	0.537594	7.4:-14709548	None:intergenic
GGTGTTGATCTCGGCGGCGA+CGG	0.540961	7.4:-14709415	None:intergenic
CGGTTGTGTTTGCAATGGTA+GGG	0.541717	7.4:-14709584	None:intergenic
GACGGTGATGGCTCGTAAAG+TGG	0.542189	7.4:-14709445	None:intergenic
GACCGAGTTGCAATTGTCAC+CGG	0.545725	7.4:+14709294	MS.gene50522:CDS
ACTGCGAATTGCGTGGCACC+GGG	0.546223	7.4:+14709831	MS.gene50522:CDS
ACTTCTGACATCGTCGTTGG+TGG	0.547144	7.4:+14709710	MS.gene50522:CDS
ATCGGACACGTCAGCGCGGA+CGG	0.549310	7.4:-14709463	None:intergenic
GGCAGCGTTGAAGATTTACA+TGG	0.549615	7.4:+14709731	MS.gene50522:CDS
ATTGTCACCGGTTCCTCCCG+CGG	0.553481	7.4:+14709306	MS.gene50522:CDS
GGCGACGGAATCAGCATGGT+TGG	0.553601	7.4:-14709400	None:intergenic
AATCGTTTGAAGCGAGGCGG+AGG	0.558244	7.4:+14709675	MS.gene50522:CDS
GATACGATCGAAGCTTTCAA+CGG	0.559331	7.4:-14709604	None:intergenic
CGATTTGAAGGCTTCTTCGG+CGG	0.570868	7.4:-14709514	None:intergenic
GTTTCACCAAGTCTTCCAAA+CGG	0.571998	7.4:-14709924	None:intergenic
GGCGAAGGAGTTGAAGGGAA+CGG	0.574740	7.4:+14709803	MS.gene50522:CDS
CAAATTATTCGTGTTAATGG+TGG	0.579717	7.4:+14710008	MS.gene50522:CDS
GATCTCTTTACCGATTCCGC+GGG	0.582675	7.4:-14709322	None:intergenic
GTACTTGGCGTCGAGGACGC+CGG	0.586113	7.4:-14709562	None:intergenic
AGCTTACACGGCGTCAAAGG+CGG	0.593385	7.4:+14709755	MS.gene50522:CDS
GTCTTGCGCCGAGTGAAGCA+AGG	0.594132	7.4:-14709353	None:intergenic
GTTTGAAGCGAGGCGGAGGA+GGG	0.595125	7.4:+14709679	MS.gene50522:CDS
CGAAGGAGTTGAAGGGAACG+GGG	0.600148	7.4:+14709805	MS.gene50522:CDS
GATGACGAAGATTCTGGCGA+AGG	0.603756	7.4:+14709788	MS.gene50522:CDS
TTCAACGGTTGTGTTTGCAA+TGG	0.605622	7.4:-14709589	None:intergenic
CATACTTCTGACATCGTCGT+TGG	0.607949	7.4:+14709707	MS.gene50522:CDS
GCCGAGTGAAGCAAGGTGGA+GGG	0.608469	7.4:-14709346	None:intergenic
ATTAACCCATTCAGAAGCAT+CGG	0.608760	7.4:-14709982	None:intergenic
CGATCTCTTTACCGATTCCG+CGG	0.612444	7.4:-14709323	None:intergenic
TGGTAGGGTACTTGGCGTCG+AGG	0.626144	7.4:-14709569	None:intergenic
GGGGATTACTGCGAATTGCG+TGG	0.628146	7.4:+14709824	MS.gene50522:CDS
GCATCGAATCGTTTGAAGCG+AGG	0.634296	7.4:+14709669	MS.gene50522:CDS
ATTGCAACTCGGTCTTTAAG+CGG	0.637282	7.4:-14709285	None:intergenic
CGGCGGCGACGGAATCAGCA+TGG	0.639400	7.4:-14709404	None:intergenic
AACCGGTGACAATTGCAACT+CGG	0.641340	7.4:-14709296	None:intergenic
GCTGACGTGTCCGATCCCGC+CGG	0.644503	7.4:+14709471	MS.gene50522:CDS
AGGAGCGTTTCTCTGCGCGA+AGG	0.650712	7.4:+14709644	MS.gene50522:CDS
TTGCGCCGAGTGAAGCAAGG+TGG	0.675152	7.4:-14709350	None:intergenic
GGCACCGGGACCGATTGCGA+CGG	0.679608	7.4:+14709845	MS.gene50522:CDS
GCAGCGTTGAAGATTTACAT+GGG	0.682047	7.4:+14709732	MS.gene50522:CDS
TCGAATCGTTTGAAGCGAGG+CGG	0.701338	7.4:+14709672	MS.gene50522:CDS
ATTCTGGCGAAGGAGTTGAA+GGG	0.705622	7.4:+14709798	MS.gene50522:CDS
CGGGATCGGACACGTCAGCG+CGG	0.767055	7.4:-14709467	None:intergenic
TTGGTTGGTGTTGATCTCGG+CGG	0.767522	7.4:-14709421	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATCATGAACATAAACTTAAA+AGG	+	chr7.4:14709624-14709643	MS.gene50522:CDS	20.0%
	CAAATTATTCGTGTTAATGG+TGG	+	chr7.4:14710008-14710027	MS.gene50522:CDS	30.0%
	GGTCAAATTATTCGTGTTAA+TGG	+	chr7.4:14710005-14710024	MS.gene50522:CDS	30.0%
!!!	GATGTTTTTTGATGGGAAAA+CGG	+	chr7.4:14709869-14709888	MS.gene50522:CDS	30.0%
!	ATTAACCCATTCAGAAGCAT+CGG	-	chr7.4:14709985-14710004	None:intergenic	35.0%
	AAAAACATCTCCGTCGCAAT+CGG	-	chr7.4:14709858-14709877	None:intergenic	40.0%
	AAAGATGTTGCTCCTGTTGT+TGG	+	chr7.4:14709948-14709967	MS.gene50522:CDS	40.0%
	ATTGCAACTCGGTCTTTAAG+CGG	-	chr7.4:14709288-14709307	None:intergenic	40.0%
	GATACGATCGAAGCTTTCAA+CGG	-	chr7.4:14709607-14709626	None:intergenic	40.0%
	GATTTACATGGGAGCTTACA+CGG	+	chr7.4:14709743-14709762	MS.gene50522:CDS	40.0%
	GTAGCCAAAAAACCAACAAC+AGG	-	chr7.4:14709963-14709982	None:intergenic	40.0%
	GTTTCACCAAGTCTTCCAAA+CGG	-	chr7.4:14709927-14709946	None:intergenic	40.0%
	TTCAACGGTTGTGTTTGCAA+TGG	-	chr7.4:14709592-14709611	None:intergenic	40.0%
!	GCAGCGTTGAAGATTTACAT+GGG	+	chr7.4:14709732-14709751	MS.gene50522:CDS	40.0%
!	GTCGAAGAGAGATTTTACAC+CGG	-	chr7.4:14709493-14709512	None:intergenic	40.0%
!!	GATGCTTCTGAATGGGTTAA+TGG	+	chr7.4:14709984-14710003	MS.gene50522:CDS	40.0%
!!!	CGACGGAGATGTTTTTTGAT+GGG	+	chr7.4:14709862-14709881	MS.gene50522:CDS	40.0%
!!!	GTTTTTTGATGGGAAAACGG+AGG	+	chr7.4:14709872-14709891	MS.gene50522:CDS	40.0%
!!!	TGCTCCTGTTGTTGGTTTTT+TGG	+	chr7.4:14709956-14709975	MS.gene50522:CDS	40.0%
	AACCGGTGACAATTGCAACT+CGG	-	chr7.4:14709299-14709318	None:intergenic	45.0%
	ACGGTTGTGTTTGCAATGGT+AGG	-	chr7.4:14709588-14709607	None:intergenic	45.0%
	ACTGAAGAAGAGAGTCCGTT+TGG	+	chr7.4:14709909-14709928	MS.gene50522:CDS	45.0%
	AGGCGATTTGAAGGCTTCTT+CGG	-	chr7.4:14709520-14709539	None:intergenic	45.0%
	ATTCTGGCGAAGGAGTTGAA+GGG	+	chr7.4:14709798-14709817	MS.gene50522:CDS	45.0%
	CATACTTCTGACATCGTCGT+TGG	+	chr7.4:14709707-14709726	MS.gene50522:CDS	45.0%
	CGGTTGTGTTTGCAATGGTA+GGG	-	chr7.4:14709587-14709606	None:intergenic	45.0%
	GAGTTAACCAGGATGTGAAC+AGG	-	chr7.4:14709540-14709559	None:intergenic	45.0%
	GATGTGAACAGGCGATTTGA+AGG	-	chr7.4:14709529-14709548	None:intergenic	45.0%
	TGATGGCTCGTAAAGTGGTT+TGG	-	chr7.4:14709443-14709462	None:intergenic	45.0%
!	AAGAGAGATTTTACACCGGC+GGG	-	chr7.4:14709489-14709508	None:intergenic	45.0%
!	GAAGAGAGATTTTACACCGG+CGG	-	chr7.4:14709490-14709509	None:intergenic	45.0%
!	GGCAGCGTTGAAGATTTACA+TGG	+	chr7.4:14709731-14709750	MS.gene50522:CDS	45.0%
!	GTTTGCAATGGTAGGGTACT+TGG	-	chr7.4:14709580-14709599	None:intergenic	45.0%
!!	GGTTTGGTTGGTGTTGATCT+CGG	-	chr7.4:14709427-14709446	None:intergenic	45.0%
!!!	GCGACGGAGATGTTTTTTGA+TGG	+	chr7.4:14709861-14709880	MS.gene50522:CDS	45.0%
	ACTTCTGACATCGTCGTTGG+TGG	+	chr7.4:14709710-14709729	MS.gene50522:CDS	50.0%
	ATCAGCATGGTTGGAGTTGG+AGG	-	chr7.4:14709394-14709413	None:intergenic	50.0%
	CAAATCGCCTGTTCACATCC+TGG	+	chr7.4:14709530-14709549	MS.gene50522:CDS	50.0%
	CACATCCTGGTTAACTCAGC+CGG	+	chr7.4:14709543-14709562	MS.gene50522:CDS	50.0%
	CGATTTGAAGGCTTCTTCGG+CGG	-	chr7.4:14709517-14709536	None:intergenic	50.0%
	GACCGAGTTGCAATTGTCAC+CGG	+	chr7.4:14709294-14709313	MS.gene50522:CDS	50.0%
	GAGAGTCCGTTTGGAAGACT+TGG	+	chr7.4:14709918-14709937	MS.gene50522:CDS	50.0%
	GATGACGAAGATTCTGGCGA+AGG	+	chr7.4:14709788-14709807	MS.gene50522:CDS	50.0%
	GATTCTGGCGAAGGAGTTGA+AGG	+	chr7.4:14709797-14709816	MS.gene50522:CDS	50.0%
	GGAAGCGATGACGAAGATTC+TGG	+	chr7.4:14709782-14709801	MS.gene50522:CDS	50.0%
	GGAATCAGCATGGTTGGAGT+TGG	-	chr7.4:14709397-14709416	None:intergenic	50.0%
	GGCTACCGATGCTTCTGAAT+GGG	+	chr7.4:14709977-14709996	MS.gene50522:CDS	50.0%
	TGATGGGAAAACGGAGGAGA+TGG	+	chr7.4:14709878-14709897	MS.gene50522:CDS	50.0%
	TGGCTACCGATGCTTCTGAA+TGG	+	chr7.4:14709976-14709995	MS.gene50522:CDS	50.0%
!	AGATTTTACACCGGCGGGAT+CGG	-	chr7.4:14709484-14709503	None:intergenic	50.0%
!	GCATCGAATCGTTTGAAGCG+AGG	+	chr7.4:14709669-14709688	MS.gene50522:CDS	50.0%
!	TCGAATCGTTTGAAGCGAGG+CGG	+	chr7.4:14709672-14709691	MS.gene50522:CDS	50.0%
!!	CGATCTCTTTACCGATTCCG+CGG	-	chr7.4:14709326-14709345	None:intergenic	50.0%
!!	GATCTCTTTACCGATTCCGC+GGG	-	chr7.4:14709325-14709344	None:intergenic	50.0%
!!	GGCTCGTAAAGTGGTTTGGT+TGG	-	chr7.4:14709439-14709458	None:intergenic	50.0%
!!	TTGGTTGGTGTTGATCTCGG+CGG	-	chr7.4:14709424-14709443	None:intergenic	50.0%
	AGCTTACACGGCGTCAAAGG+CGG	+	chr7.4:14709755-14709774	MS.gene50522:CDS	55.0%
	CGAAGGAGTTGAAGGGAACG+GGG	+	chr7.4:14709805-14709824	MS.gene50522:CDS	55.0%
	GCGAAGGAGTTGAAGGGAAC+GGG	+	chr7.4:14709804-14709823	MS.gene50522:CDS	55.0%
	GGCGAAGGAGTTGAAGGGAA+CGG	+	chr7.4:14709803-14709822	MS.gene50522:CDS	55.0%
	GGGGATTACTGCGAATTGCG+TGG	+	chr7.4:14709824-14709843	MS.gene50522:CDS	55.0%
	TACTGCGAATTGCGTGGCAC+CGG	+	chr7.4:14709830-14709849	MS.gene50522:CDS	55.0%
!	AATCGTTTGAAGCGAGGCGG+AGG	+	chr7.4:14709675-14709694	MS.gene50522:CDS	55.0%
!	GACGGTGATGGCTCGTAAAG+TGG	-	chr7.4:14709448-14709467	None:intergenic	55.0%
	ACTGCGAATTGCGTGGCACC+GGG	+	chr7.4:14709831-14709850	MS.gene50522:CDS	60.0%
	ATCTCCGTCGCAATCGGTCC+CGG	-	chr7.4:14709852-14709871	None:intergenic	60.0%
	GCCCTCCACCTTGCTTCACT+CGG	+	chr7.4:14709345-14709364	MS.gene50522:CDS	60.0%
	GCCGAGTGAAGCAAGGTGGA+GGG	-	chr7.4:14709349-14709368	None:intergenic	60.0%
	GGCGACGGAATCAGCATGGT+TGG	-	chr7.4:14709403-14709422	None:intergenic	60.0%
	GGGAGCTTACACGGCGTCAA+AGG	+	chr7.4:14709752-14709771	MS.gene50522:CDS	60.0%
	GTCTTGCGCCGAGTGAAGCA+AGG	-	chr7.4:14709356-14709375	None:intergenic	60.0%
	TTGCGCCGAGTGAAGCAAGG+TGG	-	chr7.4:14709353-14709372	None:intergenic	60.0%
!	GTTTGAAGCGAGGCGGAGGA+GGG	+	chr7.4:14709679-14709698	MS.gene50522:CDS	60.0%
!	TGGTAGGGTACTTGGCGTCG+AGG	-	chr7.4:14709572-14709591	None:intergenic	60.0%
!!	AGGAGCGTTTCTCTGCGCGA+AGG	+	chr7.4:14709644-14709663	MS.gene50522:CDS	60.0%
!!	ATTGTCACCGGTTCCTCCCG+CGG	+	chr7.4:14709306-14709325	MS.gene50522:CDS	60.0%
!!	CTCTTTACCGATTCCGCGGG+AGG	-	chr7.4:14709322-14709341	None:intergenic	60.0%
	ATCGGACACGTCAGCGCGGA+CGG	-	chr7.4:14709466-14709485	None:intergenic	65.0%
	CGCCGAGTGAAGCAAGGTGG+AGG	-	chr7.4:14709350-14709369	None:intergenic	65.0%
!	ACCGATTCCGCGGGAGGAAC+CGG	-	chr7.4:14709316-14709335	None:intergenic	65.0%
!	ACCGGTTCCTCCCGCGGAAT+CGG	+	chr7.4:14709312-14709331	MS.gene50522:CDS	65.0%
!	CGTTTGAAGCGAGGCGGAGG+AGG	+	chr7.4:14709678-14709697	MS.gene50522:CDS	65.0%
!	GGACGCCGGCTGAGTTAACC+AGG	-	chr7.4:14709551-14709570	None:intergenic	65.0%
!	GTACTTGGCGTCGAGGACGC+CGG	-	chr7.4:14709565-14709584	None:intergenic	65.0%
!!	GGTGTTGATCTCGGCGGCGA+CGG	-	chr7.4:14709418-14709437	None:intergenic	65.0%
	CACGGCGTCAAAGGCGGCTG+TGG	+	chr7.4:14709761-14709780	MS.gene50522:CDS	70.0%
	CACGTCAGCGCGGACGGTGA+TGG	-	chr7.4:14709460-14709479	None:intergenic	70.0%
	CGGCGGCGACGGAATCAGCA+TGG	-	chr7.4:14709407-14709426	None:intergenic	70.0%
	CGGGATCGGACACGTCAGCG+CGG	-	chr7.4:14709470-14709489	None:intergenic	70.0%
	GCTGACGTGTCCGATCCCGC+CGG	+	chr7.4:14709471-14709490	MS.gene50522:CDS	70.0%
!	GGCACCGGGACCGATTGCGA+CGG	+	chr7.4:14709845-14709864	MS.gene50522:CDS	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.4	gene	14709273	14710040	14709273	ID=MS.gene50522
chr7.4	mRNA	14709273	14710040	14709273	ID=MS.gene50522.t1;Parent=MS.gene50522
chr7.4	exon	14709273	14710040	14709273	ID=MS.gene50522.t1.exon1;Parent=MS.gene50522.t1
chr7.4	CDS	14709273	14710040	14709273	ID=cds.MS.gene50522.t1;Parent=MS.gene50522.t1

Gene Sequence

>MS.gene50522

ATGGCTTCGCTACCGCTTAAAGACCGAGTTGCAATTGTCACCGGTTCCTCCCGCGGAATCGGTAAAGAGATCGCCCTCCACCTTGCTTCACTCGGCGCAAGACTCGTCATCAACTACACCTCCAACTCCAACCATGCTGATTCCGTCGCCGCCGAGATCAACACCAACCAAACCACTTTACGAGCCATCACCGTCCGCGCTGACGTGTCCGATCCCGCCGGTGTAAAATCTCTCTTCGACTCCGCCGAAGAAGCCTTCAAATCGCCTGTTCACATCCTGGTTAACTCAGCCGGCGTCCTCGACGCCAAGTACCCTACCATTGCAAACACAACCGTTGAAAGCTTCGATCGTATCATGAACATAAACTTAAAAGGAGCGTTTCTCTGCGCGAAGGAAGCATCGAATCGTTTGAAGCGAGGCGGAGGAGGGAGAATCATACTTCTGACATCGTCGTTGGTGGCAGCGTTGAAGATTTACATGGGAGCTTACACGGCGTCAAAGGCGGCTGTGGAAGCGATGACGAAGATTCTGGCGAAGGAGTTGAAGGGAACGGGGATTACTGCGAATTGCGTGGCACCGGGACCGATTGCGACGGAGATGTTTTTTGATGGGAAAACGGAGGAGATGGTGAAGAAAACTGAAGAAGAGAGTCCGTTTGGAAGACTTGGTGAAACTAAAGATGTTGCTCCTGTTGTTGGTTTTTTGGCTACCGATGCTTCTGAATGGGTTAATGGTCAAATTATTCGTGTTAATGGTGGCTATGTTTAG

Protein sequence

>MS.gene50522.t1

MASLPLKDRVAIVTGSSRGIGKEIALHLASLGARLVINYTSNSNHADSVAAEINTNQTTLRAITVRADVSDPAGVKSLFDSAEEAFKSPVHILVNSAGVLDAKYPTIANTTVESFDRIMNINLKGAFLCAKEASNRLKRGGGGRIILLTSSLVAALKIYMGAYTASKAAVEAMTKILAKELKGTGITANCVAPGPIATEMFFDGKTEEMVKKTEEESPFGRLGETKDVAPVVGFLATDASEWVNGQIIRVNGGYV