AlfalfaGEDB | MS.gene42114

M. sativa cultivar XinJiangDaYe / MS.gene42114

KEGG: -
GO: -
PFAM: PF13561.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene42114.t1	XP_003623493.1	97.4	303	7	1	1	303	1	302	2.70E-162	581.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene42114.t1	Q9SCU0	66.4	301	80	6	13	303	14	303	9.8e-105	381.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene42114.t1	G7KV46	97.4	303	7	1	1	303	1	302	1.9e-162	581.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene62194	MS.gene42114	PPI
MS.gene63307	MS.gene42114	PPI
MS.gene006617	MS.gene42114	PPI
MS.gene54598	MS.gene42114	PPI
MS.gene028938	MS.gene42114	PPI
MS.gene73088	MS.gene42114	PPI
MS.gene056339	MS.gene42114	PPI
MS.gene03319	MS.gene42114	PPI
MS.gene42114	MS.gene50524	PPI
MS.gene031693	MS.gene42114	PPI
MS.gene050432	MS.gene42114	PPI
MS.gene42260	MS.gene42114	PPI
MS.gene08694	MS.gene42114	PPI
MS.gene064756	MS.gene42114	PPI
MS.gene003926	MS.gene42114	PPI
MS.gene52742	MS.gene42114	PPI
MS.gene007006	MS.gene42114	PPI
MS.gene008161	MS.gene42114	PPI
MS.gene50522	MS.gene42114	PPI
MS.gene42114	MS.gene04700	PPI
MS.gene42114	MS.gene31507	PPI
MS.gene28847	MS.gene42114	PPI
MS.gene007678	MS.gene42114	PPI
MS.gene42114	MS.gene08695	PPI
MS.gene044694	MS.gene42114	PPI
MS.gene63484	MS.gene42114	PPI
MS.gene63300	MS.gene42114	PPI
MS.gene008162	MS.gene42114	PPI
MS.gene006999	MS.gene42114	PPI
MS.gene021164	MS.gene42114	PPI
MS.gene008160	MS.gene42114	PPI
MS.gene95020	MS.gene42114	PPI
MS.gene065380	MS.gene42114	PPI
MS.gene63403	MS.gene42114	PPI
MS.gene019786	MS.gene42114	PPI
MS.gene52743	MS.gene42114	PPI
MS.gene043387	MS.gene42114	PPI
MS.gene25667	MS.gene42114	PPI
MS.gene08756	MS.gene42114	PPI
MS.gene85724	MS.gene42114	PPI
MS.gene08695	MS.gene42114	PPI
MS.gene63306	MS.gene42114	PPI
MS.gene62187	MS.gene42114	PPI
MS.gene80360	MS.gene42114	PPI
MS.gene024269	MS.gene42114	PPI
MS.gene033685	MS.gene42114	PPI
MS.gene42114	MS.gene039828	PPI
MS.gene014425	MS.gene42114	PPI
MS.gene63482	MS.gene42114	PPI
MS.gene63483	MS.gene42114	PPI
MS.gene045581	MS.gene42114	PPI
MS.gene007002	MS.gene42114	PPI
MS.gene42114	MS.gene003992	PPI
MS.gene42114	MS.gene30551	PPI
MS.gene28848	MS.gene42114	PPI
MS.gene42114	MS.gene055846	PPI
MS.gene050431	MS.gene42114	PPI
MS.gene050131	MS.gene42114	PPI
MS.gene003925	MS.gene42114	PPI
MS.gene62193	MS.gene42114	PPI
MS.gene41122	MS.gene42114	PPI
MS.gene42114	MS.gene73824	PPI
MS.gene073684	MS.gene42114	PPI
MS.gene63308	MS.gene42114	PPI
MS.gene07096	MS.gene42114	PPI
MS.gene42114	MS.gene003729	PPI
MS.gene54952	MS.gene42114	PPI
MS.gene62195	MS.gene42114	PPI
MS.gene42114	MS.gene43481	PPI
MS.gene008153	MS.gene42114	PPI
MS.gene23019	MS.gene42114	PPI
MS.gene25674	MS.gene42114	PPI
MS.gene012794	MS.gene42114	PPI
MS.gene42114	MS.gene07097	PPI
MS.gene018066	MS.gene42114	PPI
MS.gene072664	MS.gene42114	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene42114.t1	MTR_7g071680	97.360	303	7	1	1	303	1	302	0.0	598
MS.gene42114.t1	MTR_4g052340	52.920	274	105	5	27	299	12	262	2.75e-92	275
MS.gene42114.t1	MTR_4g052350	52.518	278	106	5	27	301	12	266	3.16e-91	272
MS.gene42114.t1	MTR_4g052300	51.087	276	111	4	25	299	4	256	4.81e-88	264
MS.gene42114.t1	MTR_4g052320	50.179	279	110	4	25	299	4	257	7.21e-86	258
MS.gene42114.t1	MTR_7g107670	44.138	290	147	2	10	299	14	288	5.67e-85	257
MS.gene42114.t1	MTR_7g083870	49.084	273	125	4	27	299	33	291	1.38e-82	251
MS.gene42114.t1	MTR_7g105460	49.442	269	115	3	28	295	12	260	2.97e-81	247
MS.gene42114.t1	MTR_2g090165	50.357	280	114	5	25	301	2	259	4.81e-81	246
MS.gene42114.t1	MTR_3g020670	50.173	289	127	5	16	301	2	276	1.08e-80	246
MS.gene42114.t1	MTR_7g110130	49.462	279	119	4	25	301	2	260	4.72e-79	241
MS.gene42114.t1	MTR_7g110140	48.540	274	118	4	28	299	5	257	2.72e-77	236
MS.gene42114.t1	MTR_4g078630	46.263	281	133	6	25	303	15	279	4.68e-76	234
MS.gene42114.t1	MTR_7g105580	46.324	272	107	3	27	297	6	239	6.48e-74	228
MS.gene42114.t1	MTR_7g105590	58.824	187	73	3	27	212	6	189	7.23e-72	220
MS.gene42114.t1	MTR_7g110160	47.985	273	101	5	25	295	2	235	2.31e-69	216
MS.gene42114.t1	MTR_6g023590	44.815	270	126	6	28	294	12	261	6.21e-68	213
MS.gene42114.t1	MTR_6g035395	44.891	274	117	4	27	299	3	243	4.11e-67	210
MS.gene42114.t1	MTR_6g023910	45.221	272	126	7	24	292	8	259	2.47e-66	209
MS.gene42114.t1	MTR_6g023990	42.491	273	134	6	25	294	10	262	1.51e-64	204
MS.gene42114.t1	MTR_6g023610	43.796	274	131	6	22	292	6	259	8.26e-64	202
MS.gene42114.t1	MTR_6g023950	41.497	294	136	8	4	294	1	261	5.03e-63	200
MS.gene42114.t1	MTR_6g023600	42.222	270	133	6	28	294	12	261	7.97e-61	194
MS.gene42114.t1	MTR_7g105570	50.505	198	78	2	98	295	40	217	3.93e-56	181
MS.gene42114.t1	MTR_6g023600	41.481	270	129	7	28	294	12	255	4.07e-56	182
MS.gene42114.t1	MTR_3g113870	40.455	220	116	2	37	256	29	233	1.59e-55	180
MS.gene42114.t1	MTR_4g084800	54.887	133	56	2	27	158	11	140	3.68e-44	148
MS.gene42114.t1	MTR_3g068155	33.091	275	147	7	29	295	19	264	6.03e-29	112
MS.gene42114.t1	MTR_3g068175	31.618	272	155	8	29	295	17	262	1.34e-27	108
MS.gene42114.t1	MTR_3g085740	28.920	287	154	7	22	294	68	318	2.51e-27	108
MS.gene42114.t1	MTR_3g068070	31.159	276	151	8	29	295	15	260	4.65e-27	107
MS.gene42114.t1	MTR_4g064883	31.365	271	157	7	29	295	14	259	3.53e-26	104
MS.gene42114.t1	MTR_7g094520	33.569	283	148	10	23	294	6	259	5.41e-26	103
MS.gene42114.t1	MTR_3g068085	31.408	277	149	10	29	295	15	260	7.47e-26	103
MS.gene42114.t1	MTR_3g068175	31.159	276	155	9	29	295	17	266	2.05e-25	102
MS.gene42114.t1	MTR_7g094510	33.577	274	147	8	29	293	6	253	6.18e-25	100
MS.gene42114.t1	MTR_4g132850	32.130	277	152	11	26	296	4	250	2.99e-24	99.0
MS.gene42114.t1	MTR_4g064883	34.804	204	123	5	22	220	6	204	8.64e-23	94.4
MS.gene42114.t1	MTR_7g113650	27.723	303	173	10	7	293	16	288	2.47e-22	94.7
MS.gene42114.t1	MTR_3g068065	28.777	278	159	8	29	297	17	264	2.86e-22	94.4
MS.gene42114.t1	MTR_1g082760	28.214	280	157	9	28	293	39	288	3.26e-21	91.7
MS.gene42114.t1	MTR_8g442750	30.137	292	166	8	19	298	4	269	5.43e-21	91.3
MS.gene42114.t1	MTR_1196s0010	29.520	271	157	9	27	293	272	512	1.23e-20	92.0
MS.gene42114.t1	MTR_7g063330	28.676	272	168	6	29	296	15	264	6.56e-20	87.4
MS.gene42114.t1	MTR_4g064887	26.389	288	181	7	20	303	6	266	1.22e-19	87.0
MS.gene42114.t1	MTR_4g131740	27.240	279	164	8	28	294	39	290	3.23e-19	86.3
MS.gene42114.t1	MTR_4g058840	30.526	285	160	10	26	295	2	263	7.15e-19	84.7
MS.gene42114.t1	MTR_4g058820	30.526	285	160	10	26	295	2	263	7.15e-19	84.7
MS.gene42114.t1	MTR_7g063270	30.769	286	168	11	18	296	3	265	1.10e-18	84.3
MS.gene42114.t1	MTR_1g051165	28.975	283	164	10	27	295	12	271	1.82e-18	84.0
MS.gene42114.t1	MTR_7g063320	29.592	294	168	9	9	294	1	263	2.54e-18	83.2
MS.gene42114.t1	MTR_4g058810	28.571	280	160	9	29	295	11	263	1.02e-17	81.6
MS.gene42114.t1	MTR_1g068955	31.047	277	155	8	26	292	11	261	1.98e-17	80.9
MS.gene42114.t1	MTR_4g064887	30.392	204	133	4	20	219	6	204	2.98e-17	79.3
MS.gene42114.t1	MTR_7g063260	29.371	286	173	9	18	296	3	266	1.31e-15	75.5
MS.gene42114.t1	MTR_7g063390	27.106	273	170	6	29	295	16	265	2.08e-15	75.1
MS.gene42114.t1	MTR_7g063360	29.044	272	166	9	29	295	16	265	3.66e-14	71.6
MS.gene42114.t1	MTR_8g096580	28.500	200	134	5	29	224	45	239	1.25e-13	70.5
MS.gene42114.t1	MTR_7g105560	54.237	59	25	1	22	78	4	62	3.11e-13	66.6
MS.gene42114.t1	MTR_4g134660	30.693	202	125	8	27	220	4	198	1.01e-12	67.0
MS.gene42114.t1	MTR_4g066050	29.353	201	129	5	32	224	27	222	1.38e-12	67.0
MS.gene42114.t1	MTR_8g044240	29.596	223	133	8	12	222	59	269	4.38e-12	66.2
MS.gene42114.t1	MTR_4g097510	25.131	191	137	3	30	219	3	188	1.28e-11	64.3
MS.gene42114.t1	MTR_4g086400	29.756	205	116	5	34	219	9	204	1.39e-11	65.5
MS.gene42114.t1	MTR_7g080410	27.619	210	137	6	19	218	26	230	2.72e-11	63.5
MS.gene42114.t1	MTR_7g080410	27.619	210	137	6	19	218	26	230	2.96e-11	63.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene42114.t1	AT3G51680	66.221	299	84	5	13	303	14	303	7.38e-132	377
MS.gene42114.t1	AT3G26770	48.897	272	125	2	27	298	39	296	1.04e-85	259
MS.gene42114.t1	AT3G29250	49.451	273	115	5	25	295	2	253	2.21e-81	247
MS.gene42114.t1	AT3G29250	46.980	298	127	6	8	295	13	289	2.45e-81	248
MS.gene42114.t1	AT2G47130	50.000	270	111	5	28	295	5	252	2.29e-80	244
MS.gene42114.t1	AT3G29250	46.333	300	128	6	8	295	13	291	9.20e-80	244
MS.gene42114.t1	AT3G26760	43.003	293	148	4	7	299	21	294	1.69e-79	243
MS.gene42114.t1	AT4G03140	45.588	272	133	2	27	298	76	332	5.83e-79	243
MS.gene42114.t1	AT2G47140	47.985	273	119	4	25	295	2	253	1.25e-78	240
MS.gene42114.t1	AT3G29260	45.421	273	125	5	25	295	2	252	6.97e-73	225
MS.gene42114.t1	AT1G52340	45.324	278	133	3	27	299	16	279	1.73e-72	225
MS.gene42114.t1	AT2G47120	46.667	270	119	6	28	295	6	252	7.19e-72	223
MS.gene42114.t1	AT2G47120	46.667	270	119	6	28	295	5	251	9.44e-72	222
MS.gene42114.t1	AT3G42960	47.842	278	122	7	25	297	4	263	5.22e-65	206
MS.gene42114.t1	AT5G06060	31.387	274	153	7	29	295	9	254	1.85e-30	115
MS.gene42114.t1	AT1G24360	28.094	299	178	7	5	294	47	317	3.88e-25	102
MS.gene42114.t1	AT5G18210	32.374	278	150	9	29	294	8	259	2.00e-24	99.8
MS.gene42114.t1	AT1G63380	30.897	301	162	12	12	295	1	272	5.33e-24	99.4
MS.gene42114.t1	AT1G63380	31.449	283	149	11	29	295	15	268	3.48e-23	97.1
MS.gene42114.t1	AT1G63380	31.449	283	149	11	29	295	17	270	4.38e-23	96.7
MS.gene42114.t1	AT4G05530	32.500	280	147	12	26	296	6	252	4.70e-23	95.9
MS.gene42114.t1	AT1G63380	31.095	283	150	11	29	295	22	275	5.28e-23	96.7
MS.gene42114.t1	AT1G62610	30.405	296	170	11	12	295	1	272	6.78e-23	96.3
MS.gene42114.t1	AT3G55290	30.435	299	163	14	12	295	1	269	1.03e-22	95.5
MS.gene42114.t1	AT1G07440	29.044	272	161	7	29	295	12	256	1.26e-22	95.1
MS.gene42114.t1	AT3G55290	30.851	282	151	13	29	295	18	270	1.28e-22	95.5
MS.gene42114.t1	AT2G29260	31.250	272	156	8	29	295	68	313	2.20e-22	95.5
MS.gene42114.t1	AT3G03980	30.201	298	162	10	12	293	1	268	2.33e-22	94.4
MS.gene42114.t1	AT1G62610	30.935	278	157	10	29	295	15	268	4.45e-22	94.0
MS.gene42114.t1	AT1G62610	30.935	278	157	10	29	295	13	266	4.81e-22	93.6
MS.gene42114.t1	AT1G62610	30.935	278	157	10	29	295	14	267	4.94e-22	93.6
MS.gene42114.t1	AT3G04000	31.525	295	152	10	19	293	5	269	5.44e-22	93.6
MS.gene42114.t1	AT5G18210	33.206	262	137	9	29	278	8	243	1.06e-21	92.8
MS.gene42114.t1	AT2G29290	29.286	280	151	8	29	295	7	252	2.67e-21	91.3
MS.gene42114.t1	AT2G29330	26.937	271	169	6	29	295	7	252	3.91e-21	90.9
MS.gene42114.t1	AT2G30670	26.937	271	169	5	29	295	7	252	5.06e-21	90.5
MS.gene42114.t1	AT2G17845	30.142	282	152	11	29	293	47	300	6.25e-21	91.3
MS.gene42114.t1	AT1G07450	28.309	272	163	7	29	295	8	252	6.62e-21	90.1
MS.gene42114.t1	AT3G46170	30.249	281	154	12	29	295	26	278	9.47e-21	90.5
MS.gene42114.t1	AT2G29310	28.467	274	161	7	29	295	7	252	1.22e-20	89.7
MS.gene42114.t1	AT4G13180	32.000	275	152	8	29	294	13	261	1.24e-20	89.7
MS.gene42114.t1	AT2G29340	27.737	274	163	7	29	295	7	252	2.04e-20	89.0
MS.gene42114.t1	AT2G29340	27.737	274	163	7	29	295	7	252	3.97e-20	89.0
MS.gene42114.t1	AT3G12800	29.932	294	159	8	22	298	3	266	5.07e-20	88.6
MS.gene42114.t1	AT2G29320	29.749	279	152	9	29	295	13	259	9.03e-20	87.4
MS.gene42114.t1	AT3G55310	30.526	285	148	14	29	295	17	269	1.02e-19	87.4
MS.gene42114.t1	AT2G29300	27.536	276	162	7	29	295	7	253	1.11e-19	87.0
MS.gene42114.t1	AT2G29370	28.881	277	163	8	26	295	12	261	1.36e-19	86.7
MS.gene42114.t1	AT2G29300	27.305	282	167	7	29	301	7	259	2.11e-19	86.7
MS.gene42114.t1	AT3G05260	26.071	280	164	7	28	294	36	285	2.34e-19	86.7
MS.gene42114.t1	AT1G54870	25.926	297	174	10	11	293	66	330	3.11e-19	87.0
MS.gene42114.t1	AT1G54870	25.926	297	174	10	11	293	68	332	3.68e-19	86.7
MS.gene42114.t1	AT2G29350	29.304	273	159	7	29	295	15	259	6.32e-19	85.1
MS.gene42114.t1	AT1G10310	31.905	210	136	3	19	226	5	209	6.42e-19	84.3
MS.gene42114.t1	AT2G29360	27.273	275	163	6	29	295	16	261	8.12e-19	84.7
MS.gene42114.t1	AT2G29310	32.828	198	118	5	29	219	7	196	3.38e-18	81.6
MS.gene42114.t1	AT2G29340	31.937	191	115	5	29	212	7	189	1.14e-17	80.1
MS.gene42114.t1	AT2G29340	31.937	191	115	5	29	212	7	189	1.14e-17	80.1
MS.gene42114.t1	AT2G29290	29.439	214	123	6	84	295	48	235	1.19e-16	78.2
MS.gene42114.t1	AT2G29320	33.333	198	116	6	29	218	13	202	3.17e-16	76.3
MS.gene42114.t1	AT2G29350	31.472	197	122	4	29	219	15	204	2.56e-15	73.9
MS.gene42114.t1	AT2G29350	28.638	213	125	5	84	295	35	221	4.28e-14	70.9
MS.gene42114.t1	AT2G29310	26.459	257	154	7	46	295	22	250	5.01e-14	71.2
MS.gene42114.t1	AT2G29320	27.863	262	145	9	46	295	12	241	2.73e-13	68.9
MS.gene42114.t1	AT5G50600	26.457	223	138	6	21	231	37	245	5.16e-13	68.9
MS.gene42114.t1	AT5G50700	26.457	223	138	6	21	231	37	245	5.16e-13	68.9
MS.gene42114.t1	AT5G04900	30.457	197	125	4	35	222	83	276	6.82e-12	65.9
MS.gene42114.t1	AT5G50690	30.387	181	111	5	27	200	43	215	1.48e-11	63.9
MS.gene42114.t1	AT5G50590	30.387	181	111	5	27	200	43	215	1.48e-11	63.9
MS.gene42114.t1	AT5G50690	30.387	181	111	5	27	200	43	215	1.52e-11	64.3
MS.gene42114.t1	AT5G50590	30.387	181	111	5	27	200	43	215	1.52e-11	64.3
MS.gene42114.t1	AT2G29310	30.387	181	111	5	46	219	22	194	2.42e-11	62.4
MS.gene42114.t1	AT3G03330	28.646	192	125	6	29	212	41	228	2.93e-11	63.5
MS.gene42114.t1	AT1G07440	31.902	163	100	5	29	186	12	168	9.09e-11	60.1

Find 54 sgRNAs with CRISPR-Local

Find 88 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GTTGATGAGGGAATCAATTT+TGG	0.168629	7.3:-32961403	MS.gene42114:CDS
TTCAACAATGCTGGCTTTCT+TGG	0.183584	7.3:-32961766	MS.gene42114:CDS
TTTGAGAGGAACAACTTTAA+AGG	0.232093	7.3:-32961323	MS.gene42114:CDS
AAAGAATGCTTCTTGTGAAT+TGG	0.241146	7.3:-32961515	MS.gene42114:CDS
GTCATGAGTGTTAATGTTAA+AGG	0.281504	7.3:-32961685	MS.gene42114:CDS
GAGGAATTTGTTAGAGGAAT+TGG	0.288716	7.3:-32961349	MS.gene42114:CDS
AATTGGGAAAGTATGGTATA+AGG	0.304183	7.3:-32961498	MS.gene42114:CDS
AATGTTAAAGGAGTTGCTTT+AGG	0.341836	7.3:-32961673	MS.gene42114:CDS
GTTAATTGCATTTCTCCATT+TGG	0.358113	7.3:-32961475	MS.gene42114:CDS
AAGAATGCTTCTTGTGAATT+GGG	0.359321	7.3:-32961514	MS.gene42114:CDS
TCTTGTGAATTGGGAAAGTA+TGG	0.368569	7.3:-32961505	MS.gene42114:CDS
GTTATGATACCTAGAGGAAT+TGG	0.379336	7.3:-32961631	MS.gene42114:CDS
ACAGGTGGAGCTAGAGGAAT+TGG	0.380505	7.3:-32961985	MS.gene42114:CDS
ACTTCAACACTTTCTCACTA+TGG	0.383031	7.3:-32961805	MS.gene42114:CDS
CATGGTGCAAAAGTGGTAAT+TGG	0.384110	7.3:-32961934	MS.gene42114:CDS
CTTGTGAATGCTTGGAGAAA+TGG	0.386066	7.3:-32961436	MS.gene42114:CDS
GTGAAGAGAAAGTTCTTGAA+GGG	0.396504	7.3:+32962425	None:intergenic
AAAATGGAGGAATTTGTTAG+AGG	0.408023	7.3:-32961355	MS.gene42114:CDS
GCTTCAAAGCATGCTATAGT+TGG	0.409180	7.3:-32961544	MS.gene42114:CDS
ACTTCCTCAAGAAATTGTAT+TGG	0.410994	7.3:-32961211	MS.gene42114:CDS
ATTTCCACTTCTAGTGTAGC+TGG	0.432577	7.3:-32961601	MS.gene42114:CDS
GACATAATGTTCAACAATGC+TGG	0.445419	7.3:-32961775	MS.gene42114:CDS
GATGAATCTAAGTATGTTAG+TGG	0.446032	7.3:-32961262	MS.gene42114:CDS
TTTCTCCTCCTTCACCTAAG+AGG	0.457332	7.3:-32962386	MS.gene42114:intron
CATCTATGCTTGTGAATGCT+TGG	0.461933	7.3:-32961444	MS.gene42114:CDS
ATTGGGAAAGTATGGTATAA+GGG	0.485485	7.3:-32961497	MS.gene42114:CDS
AACTCCAGCTACACTAGAAG+TGG	0.492424	7.3:+32961597	None:intergenic
GGTCATAATCTAGTTGTTGA+TGG	0.504632	7.3:-32961241	MS.gene42114:CDS
ATAGATGTTGCAACACCAAA+TGG	0.508686	7.3:+32961460	None:intergenic
AGTGAAGAGAAAGTTCTTGA+AGG	0.511591	7.3:+32962424	None:intergenic
GGTGATGTTGAAGATGAACT+TGG	0.516407	7.3:-32961913	MS.gene42114:CDS
TGAAGAGAAAGTTCTTGAAG+GGG	0.527961	7.3:+32962426	None:intergenic
AGAGGAATTGGTAATTTGAG+AGG	0.537792	7.3:-32961337	MS.gene42114:CDS
AGGAATGAAACATGCTGCAA+AGG	0.544045	7.3:-32961653	MS.gene42114:CDS
TGGATTGCCTTTAATTGAGG+AGG	0.552107	7.3:-32961383	MS.gene42114:CDS
ATATTGTTATATAGGTTGGA+AGG	0.556000	7.3:-32962024	MS.gene42114:intron
CAATGGACATAAATAGCTGA+TGG	0.565528	7.3:+32961865	None:intergenic
TGTGAAACATGGTGCAAAAG+TGG	0.566017	7.3:-32961941	MS.gene42114:CDS
CATGACTCGGTCGAATTCCT+CGG	0.569713	7.3:+32961702	None:intergenic
AAAATAGCTATTGTAACAGG+TGG	0.571177	7.3:-32962000	MS.gene42114:CDS
TAGTACCTCTTAGGTGAAGG+AGG	0.580775	7.3:+32962381	None:intergenic
ATTATGCATCCAATTCCTCT+AGG	0.581954	7.3:+32961622	None:intergenic
CAATCCAATACAATTTCTTG+AGG	0.587313	7.3:+32961207	None:intergenic
ATTGTAACAGGTGGAGCTAG+AGG	0.598201	7.3:-32961991	MS.gene42114:CDS
TGGAGATGATGAAGTTGATG+AGG	0.599728	7.3:-32961416	MS.gene42114:CDS
AGGTGAAGGAGGAGAAAATG+TGG	0.602352	7.3:+32962392	None:intergenic
CATTGTGAACTTCGACGCCG+AGG	0.604387	7.3:-32961719	MS.gene42114:CDS
TAACATTAACACTCATGACT+CGG	0.611584	7.3:+32961689	None:intergenic
TTTCAACACTAACATCACAA+TGG	0.616635	7.3:+32961848	None:intergenic
TAGAGGAATTGGAGAAGCAA+CGG	0.616718	7.3:-32961974	MS.gene42114:CDS
GGAGATGATGAAGTTGATGA+GGG	0.618165	7.3:-32961415	MS.gene42114:CDS
CATAATCTAGTTGTTGATGG+TGG	0.619135	7.3:-32961238	MS.gene42114:CDS
GCAAAGGTTATGATACCTAG+AGG	0.690868	7.3:-32961637	MS.gene42114:CDS
TTTGAAGCTGTATAAGCATG+TGG	0.732644	7.3:+32961559	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AATATTTGATATTGTTATAT+AGG	-	chr7.3:32961619-32961638	MS.gene42114:CDS	10.0%
!!!	TTATTATTTTTACTATGTTT+TGG	-	chr7.3:32961497-32961516	MS.gene42114:CDS	10.0%
!!	AGACATTAATTATATTACTT+TGG	+	chr7.3:32961308-32961327	None:intergenic	15.0%
!!!	TAATGTCTTTTTTTTTTTTG+TGG	-	chr7.3:32961320-32961339	MS.gene42114:CDS	15.0%
!!	TACCATGAAAAATTTCATAT+TGG	-	chr7.3:32961522-32961541	MS.gene42114:CDS	20.0%
!!	TTTGATATTGTTATATAGGT+TGG	-	chr7.3:32961623-32961642	MS.gene42114:CDS	20.0%
!!!	ATCCAATATGAAATTTTTCA+TGG	+	chr7.3:32961527-32961546	None:intergenic	20.0%
!	AAGATTACTATCAATTGATG+GGG	+	chr7.3:32961568-32961587	None:intergenic	25.0%
!	CAATGCATAATATATGCAAA+AGG	+	chr7.3:32961368-32961387	None:intergenic	25.0%
!	GAAGATTACTATCAATTGAT+GGG	+	chr7.3:32961569-32961588	None:intergenic	25.0%
!	TGAAGATTACTATCAATTGA+TGG	+	chr7.3:32961570-32961589	None:intergenic	25.0%
!	TTAACTAACTACGAATCTTA+TGG	-	chr7.3:32961596-32961615	MS.gene42114:CDS	25.0%
!!	ATATTGTTATATAGGTTGGA+AGG	-	chr7.3:32961627-32961646	MS.gene42114:CDS	25.0%
!!!	TAGAAATTTTAGTACCTCTT+AGG	+	chr7.3:32961282-32961301	None:intergenic	25.0%
!!!	TGTCTTTTTTTTTTTTGTGG+TGG	-	chr7.3:32961323-32961342	MS.gene42114:CDS	25.0%
!!!	TTTTTTTTTTTTGTGGTGGA+CGG	-	chr7.3:32961327-32961346	MS.gene42114:CDS	25.0%
	AAAATAGCTATTGTAACAGG+TGG	-	chr7.3:32961651-32961670	MS.gene42114:CDS	30.0%
	AAAGAATGCTTCTTGTGAAT+TGG	-	chr7.3:32962136-32962155	MS.gene42114:intron	30.0%
	AAGAATGCTTCTTGTGAATT+GGG	-	chr7.3:32962137-32962156	MS.gene42114:intron	30.0%
	AATTGAGGAGGTTGAAAAAA+TGG	-	chr7.3:32962280-32962299	MS.gene42114:intron	30.0%
	ACTTCCTCAAGAAATTGTAT+TGG	-	chr7.3:32962440-32962459	MS.gene42114:CDS	30.0%
	CAATCCAATACAATTTCTTG+AGG	+	chr7.3:32962447-32962466	None:intergenic	30.0%
	GATGAATCTAAGTATGTTAG+TGG	-	chr7.3:32962389-32962408	MS.gene42114:CDS	30.0%
	GGAAAAATAGCTATTGTAAC+AGG	-	chr7.3:32961648-32961667	MS.gene42114:CDS	30.0%
	GTCATGAGTGTTAATGTTAA+AGG	-	chr7.3:32961966-32961985	MS.gene42114:CDS	30.0%
	GTTAATTGCATTTCTCCATT+TGG	-	chr7.3:32962176-32962195	MS.gene42114:intron	30.0%
	TAACATTAACACTCATGACT+CGG	+	chr7.3:32961965-32961984	None:intergenic	30.0%
	TTTCAACACTAACATCACAA+TGG	+	chr7.3:32961806-32961825	None:intergenic	30.0%
	TTTGAGAGGAACAACTTTAA+AGG	-	chr7.3:32962328-32962347	MS.gene42114:intron	30.0%
!	AATGTTAAAGGAGTTGCTTT+AGG	-	chr7.3:32961978-32961997	MS.gene42114:CDS	30.0%
!	AATTGGGAAAGTATGGTATA+AGG	-	chr7.3:32962153-32962172	MS.gene42114:intron	30.0%
!	ATTGGGAAAGTATGGTATAA+GGG	-	chr7.3:32962154-32962173	MS.gene42114:intron	30.0%
!	GTGAGAATTTTTGTGAAACA+TGG	-	chr7.3:32961699-32961718	MS.gene42114:CDS	30.0%
!	TTTTCAACCTCCTCAATTAA+AGG	+	chr7.3:32962278-32962297	None:intergenic	30.0%
!	TTTTGGATTGCCTTTAATTG+AGG	-	chr7.3:32962265-32962284	MS.gene42114:intron	30.0%
!!	AAAATGGAGGAATTTGTTAG+AGG	-	chr7.3:32962296-32962315	MS.gene42114:intron	30.0%
!!!	CACAATGCTTTTTTTGTTCT+TGG	+	chr7.3:32961919-32961938	None:intergenic	30.0%
!!!	TGCTTTTTTTGTTCTTGGAT+TGG	+	chr7.3:32961914-32961933	None:intergenic	30.0%
!!!	TTTTTTTTTTTGTGGTGGAC+GGG	-	chr7.3:32961328-32961347	MS.gene42114:CDS	30.0%
	ACTTCAACACTTTCTCACTA+TGG	-	chr7.3:32961846-32961865	MS.gene42114:CDS	35.0%
	AGTGAAGAGAAAGTTCTTGA+AGG	+	chr7.3:32961230-32961249	None:intergenic	35.0%
	ATAGATGTTGCAACACCAAA+TGG	+	chr7.3:32962194-32962213	None:intergenic	35.0%
	ATGAGTTTAACTCAGTTGGT+AGG	+	chr7.3:32961393-32961412	None:intergenic	35.0%
	ATTACTATCAATTGATGGGG+TGG	+	chr7.3:32961565-32961584	None:intergenic	35.0%
	ATTATGCATCCAATTCCTCT+AGG	+	chr7.3:32962032-32962051	None:intergenic	35.0%
	CAATGGACATAAATAGCTGA+TGG	+	chr7.3:32961789-32961808	None:intergenic	35.0%
	GACATAATGTTCAACAATGC+TGG	-	chr7.3:32961876-32961895	MS.gene42114:CDS	35.0%
	GGTCATAATCTAGTTGTTGA+TGG	-	chr7.3:32962410-32962429	MS.gene42114:CDS	35.0%
	GTGAAGAGAAAGTTCTTGAA+GGG	+	chr7.3:32961229-32961248	None:intergenic	35.0%
	GTTATGATACCTAGAGGAAT+TGG	-	chr7.3:32962020-32962039	MS.gene42114:intron	35.0%
	TCTCATGAGTTTAACTCAGT+TGG	+	chr7.3:32961397-32961416	None:intergenic	35.0%
	TCTTGTGAATTGGGAAAGTA+TGG	-	chr7.3:32962146-32962165	MS.gene42114:intron	35.0%
	TGAAGAGAAAGTTCTTGAAG+GGG	+	chr7.3:32961228-32961247	None:intergenic	35.0%
!	AGAGGAATTGGTAATTTGAG+AGG	-	chr7.3:32962314-32962333	MS.gene42114:intron	35.0%
!	CATAATCTAGTTGTTGATGG+TGG	-	chr7.3:32962413-32962432	MS.gene42114:CDS	35.0%
!	TGAGTTTAACTCAGTTGGTA+GGG	+	chr7.3:32961392-32961411	None:intergenic	35.0%
!	TTTGAAGCTGTATAAGCATG+TGG	+	chr7.3:32962095-32962114	None:intergenic	35.0%
!	TTTTAGTACCTCTTAGGTGA+AGG	+	chr7.3:32961276-32961295	None:intergenic	35.0%
!!	GAGGAATTTGTTAGAGGAAT+TGG	-	chr7.3:32962302-32962321	MS.gene42114:intron	35.0%
!!	GTTGATGAGGGAATCAATTT+TGG	-	chr7.3:32962248-32962267	MS.gene42114:intron	35.0%
!!!	TTTTTTTTTTGTGGTGGACG+GGG	-	chr7.3:32961329-32961348	MS.gene42114:CDS	35.0%
	AGGAATGAAACATGCTGCAA+AGG	-	chr7.3:32961998-32962017	MS.gene42114:CDS	40.0%
	ATTTCCACTTCTAGTGTAGC+TGG	-	chr7.3:32962050-32962069	MS.gene42114:intron	40.0%
	CATCTATGCTTGTGAATGCT+TGG	-	chr7.3:32962207-32962226	MS.gene42114:intron	40.0%
	CATGGTGCAAAAGTGGTAAT+TGG	-	chr7.3:32961717-32961736	MS.gene42114:CDS	40.0%
	CTTGTGAATGCTTGGAGAAA+TGG	-	chr7.3:32962215-32962234	MS.gene42114:intron	40.0%
	GCAAAGGTTATGATACCTAG+AGG	-	chr7.3:32962014-32962033	MS.gene42114:intron	40.0%
	GCTTCAAAGCATGCTATAGT+TGG	-	chr7.3:32962107-32962126	MS.gene42114:intron	40.0%
	GGTGATGTTGAAGATGAACT+TGG	-	chr7.3:32961738-32961757	MS.gene42114:CDS	40.0%
	TAGAGGAATTGGAGAAGCAA+CGG	-	chr7.3:32961677-32961696	MS.gene42114:CDS	40.0%
	TGAGGAGGTTGAAAAAATGG+AGG	-	chr7.3:32962283-32962302	MS.gene42114:intron	40.0%
	TGGATTGCCTTTAATTGAGG+AGG	-	chr7.3:32962268-32962287	MS.gene42114:intron	40.0%
	TGTGAAACATGGTGCAAAAG+TGG	-	chr7.3:32961710-32961729	MS.gene42114:CDS	40.0%
!	TCTAGTGTAGCTGGAGTTTT+AGG	-	chr7.3:32962059-32962078	MS.gene42114:intron	40.0%
!!	GGAGATGATGAAGTTGATGA+GGG	-	chr7.3:32962236-32962255	MS.gene42114:intron	40.0%
!!	TGGAGATGATGAAGTTGATG+AGG	-	chr7.3:32962235-32962254	MS.gene42114:intron	40.0%
!!	TTCAACAATGCTGGCTTTCT+TGG	-	chr7.3:32961885-32961904	MS.gene42114:CDS	40.0%
	AACTCCAGCTACACTAGAAG+TGG	+	chr7.3:32962057-32962076	None:intergenic	45.0%
	AGGTGAAGGAGGAGAAAATG+TGG	+	chr7.3:32961262-32961281	None:intergenic	45.0%
	ATTGTAACAGGTGGAGCTAG+AGG	-	chr7.3:32961660-32961679	MS.gene42114:CDS	45.0%
	TAGTACCTCTTAGGTGAAGG+AGG	+	chr7.3:32961273-32961292	None:intergenic	45.0%
	TTTCTCCTCCTTCACCTAAG+AGG	-	chr7.3:32961265-32961284	MS.gene42114:CDS	45.0%
!!!	AGTGTAGCTGGAGTTTTAGG+AGG	-	chr7.3:32962062-32962081	MS.gene42114:intron	45.0%
!!	AATAATAAAATAATAATTCA+AGG	+	chr7.3:32961443-32961462	None:intergenic	5.0%
	ACAGGTGGAGCTAGAGGAAT+TGG	-	chr7.3:32961666-32961685	MS.gene42114:CDS	50.0%
!!	CATGACTCGGTCGAATTCCT+CGG	+	chr7.3:32961952-32961971	None:intergenic	50.0%
!!!	GCTGGAGTTTTAGGAGGACT+TGG	-	chr7.3:32962068-32962087	MS.gene42114:intron	50.0%
	CATTGTGAACTTCGACGCCG+AGG	-	chr7.3:32961932-32961951	MS.gene42114:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.3	gene	32961204	32962469	32961204	ID=MS.gene42114
chr7.3	mRNA	32961204	32962469	32961204	ID=MS.gene42114.t1;Parent=MS.gene42114
chr7.3	exon	32962387	32962469	32962387	ID=MS.gene42114.t1.exon1;Parent=MS.gene42114.t1
chr7.3	CDS	32962387	32962469	32962387	ID=cds.MS.gene42114.t1;Parent=MS.gene42114.t1
chr7.3	exon	32961204	32962032	32961204	ID=MS.gene42114.t1.exon2;Parent=MS.gene42114.t1
chr7.3	CDS	32961204	32962032	32961204	ID=cds.MS.gene42114.t1;Parent=MS.gene42114.t1

Gene Sequence

>MS.gene42114

ATGGGTGTAATGTCTGAGAAACCCCTTCAAGAACTTTCTCTTCACTCTACAGACACCACATTTTCTCCTCCTTCACCTAAGAGGTACTAAAATTTCTATATCCAAAGTAATATAATTAATGTCTTTTTTTTTTTTGTGGTGGACGGGGTTTGAATCTCAGACCTTTTGCATATATTATGCATTGTCCCTACCAACTGAGTTAAACTCATGAGAACATTAATGTCTTTTTCTATTTACCTTGAATTATTATTTTATTATTATTATTATTATTATCATCATCATCATCATCATTATTATTATTTTTACTATGTTTTGGTTTACCATGAAAAATTTCATATTGGATAAGTATATGTCTCTTCCACCCCATCAATTGATAGTAATCTTCAATCTTGTTAACTAACTACGAATCTTATGGAATATTTGATATTGTTATATAGGTTGGAAGGAAAAATAGCTATTGTAACAGGTGGAGCTAGAGGAATTGGAGAAGCAACGGTGAGAATTTTTGTGAAACATGGTGCAAAAGTGGTAATTGGTGATGTTGAAGATGAACTTGGAATCATGCTTGCAAATTCACTCTCTCCATCAGCTATTTATGTCCATTGTGATGTTAGTGTTGAAAAAGATGTTGAAAATTTAGTCACTTCAACACTTTCTCACTATGGAAAACTTGACATAATGTTCAACAATGCTGGCTTTCTTGGAAACCAATCCAAGAACAAAAAAAGCATTGTGAACTTCGACGCCGAGGAATTCGACCGAGTCATGAGTGTTAATGTTAAAGGAGTTGCTTTAGGAATGAAACATGCTGCAAAGGTTATGATACCTAGAGGAATTGGATGCATAATTTCCACTTCTAGTGTAGCTGGAGTTTTAGGAGGACTTGGTCCACATGCTTATACAGCTTCAAAGCATGCTATAGTTGGATTAACAAAGAATGCTTCTTGTGAATTGGGAAAGTATGGTATAAGGGTTAATTGCATTTCTCCATTTGGTGTTGCAACATCTATGCTTGTGAATGCTTGGAGAAATGGAGATGATGAAGTTGATGAGGGAATCAATTTTGGATTGCCTTTAATTGAGGAGGTTGAAAAAATGGAGGAATTTGTTAGAGGAATTGGTAATTTGAGAGGAACAACTTTAAAGGCTCAAGATATTGCTGAAGCTGTTCTTTATCTTGCTAGTGATGAATCTAAGTATGTTAGTGGTCATAATCTAGTTGTTGATGGTGGAATTACTTCCTCAAGAAATTGTATTGGATTGTGA

Protein sequence

>MS.gene42114.t1

MGVMSEKPLQELSLHSTDTTFSPPSPKRLEGKIAIVTGGARGIGEATVRIFVKHGAKVVIGDVEDELGIMLANSLSPSAIYVHCDVSVEKDVENLVTSTLSHYGKLDIMFNNAGFLGNQSKNKKSIVNFDAEEFDRVMSVNVKGVALGMKHAAKVMIPRGIGCIISTSSVAGVLGGLGPHAYTASKHAIVGLTKNASCELGKYGIRVNCISPFGVATSMLVNAWRNGDDEVDEGINFGLPLIEEVEKMEEFVRGIGNLRGTTLKAQDIAEAVLYLASDESKYVSGHNLVVDGGITSSRNCIGL