Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42114.t1 | XP_003623493.1 | 97.4 | 303 | 7 | 1 | 1 | 303 | 1 | 302 | 2.70E-162 | 581.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42114.t1 | Q9SCU0 | 66.4 | 301 | 80 | 6 | 13 | 303 | 14 | 303 | 9.8e-105 | 381.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42114.t1 | G7KV46 | 97.4 | 303 | 7 | 1 | 1 | 303 | 1 | 302 | 1.9e-162 | 581.3 |
TFs/TRs:
Protein Kinases:
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| No data available in table | ||||
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene003925 | MS.gene42114 | PPI |
| MS.gene003926 | MS.gene42114 | PPI |
| MS.gene006617 | MS.gene42114 | PPI |
| MS.gene006999 | MS.gene42114 | PPI |
| MS.gene007002 | MS.gene42114 | PPI |
| MS.gene007006 | MS.gene42114 | PPI |
| MS.gene007678 | MS.gene42114 | PPI |
| MS.gene008153 | MS.gene42114 | PPI |
| MS.gene008160 | MS.gene42114 | PPI |
| MS.gene008161 | MS.gene42114 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42114.t1 | MTR_7g071680 | 97.360 | 303 | 7 | 1 | 1 | 303 | 1 | 302 | 0.0 | 598 |
| MS.gene42114.t1 | MTR_4g052340 | 52.920 | 274 | 105 | 5 | 27 | 299 | 12 | 262 | 2.75e-92 | 275 |
| MS.gene42114.t1 | MTR_4g052350 | 52.518 | 278 | 106 | 5 | 27 | 301 | 12 | 266 | 3.16e-91 | 272 |
| MS.gene42114.t1 | MTR_4g052300 | 51.087 | 276 | 111 | 4 | 25 | 299 | 4 | 256 | 4.81e-88 | 264 |
| MS.gene42114.t1 | MTR_4g052320 | 50.179 | 279 | 110 | 4 | 25 | 299 | 4 | 257 | 7.21e-86 | 258 |
| MS.gene42114.t1 | MTR_7g107670 | 44.138 | 290 | 147 | 2 | 10 | 299 | 14 | 288 | 5.67e-85 | 257 |
| MS.gene42114.t1 | MTR_7g083870 | 49.084 | 273 | 125 | 4 | 27 | 299 | 33 | 291 | 1.38e-82 | 251 |
| MS.gene42114.t1 | MTR_7g105460 | 49.442 | 269 | 115 | 3 | 28 | 295 | 12 | 260 | 2.97e-81 | 247 |
| MS.gene42114.t1 | MTR_2g090165 | 50.357 | 280 | 114 | 5 | 25 | 301 | 2 | 259 | 4.81e-81 | 246 |
| MS.gene42114.t1 | MTR_3g020670 | 50.173 | 289 | 127 | 5 | 16 | 301 | 2 | 276 | 1.08e-80 | 246 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42114.t1 | AT3G51680 | 66.221 | 299 | 84 | 5 | 13 | 303 | 14 | 303 | 7.38e-132 | 377 |
| MS.gene42114.t1 | AT3G26770 | 48.897 | 272 | 125 | 2 | 27 | 298 | 39 | 296 | 1.04e-85 | 259 |
| MS.gene42114.t1 | AT3G29250 | 49.451 | 273 | 115 | 5 | 25 | 295 | 2 | 253 | 2.21e-81 | 247 |
| MS.gene42114.t1 | AT3G29250 | 46.980 | 298 | 127 | 6 | 8 | 295 | 13 | 289 | 2.45e-81 | 248 |
| MS.gene42114.t1 | AT2G47130 | 50.000 | 270 | 111 | 5 | 28 | 295 | 5 | 252 | 2.29e-80 | 244 |
| MS.gene42114.t1 | AT3G29250 | 46.333 | 300 | 128 | 6 | 8 | 295 | 13 | 291 | 9.20e-80 | 244 |
| MS.gene42114.t1 | AT3G26760 | 43.003 | 293 | 148 | 4 | 7 | 299 | 21 | 294 | 1.69e-79 | 243 |
| MS.gene42114.t1 | AT4G03140 | 45.588 | 272 | 133 | 2 | 27 | 298 | 76 | 332 | 5.83e-79 | 243 |
| MS.gene42114.t1 | AT2G47140 | 47.985 | 273 | 119 | 4 | 25 | 295 | 2 | 253 | 1.25e-78 | 240 |
| MS.gene42114.t1 | AT3G29260 | 45.421 | 273 | 125 | 5 | 25 | 295 | 2 | 252 | 6.97e-73 | 225 |
Find 54 sgRNAs with CRISPR-Local
Find 88 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAATAGCTATTGTAACAGG+TGG | 0.571177 | 7.3:-32962000 | MS.gene42114:CDS |
| AAAATGGAGGAATTTGTTAG+AGG | 0.408023 | 7.3:-32961355 | MS.gene42114:CDS |
| AAAGAATGCTTCTTGTGAAT+TGG | 0.241146 | 7.3:-32961515 | MS.gene42114:CDS |
| AACTCCAGCTACACTAGAAG+TGG | 0.492424 | 7.3:+32961597 | None:intergenic |
| AAGAATGCTTCTTGTGAATT+GGG | 0.359321 | 7.3:-32961514 | MS.gene42114:CDS |
| AATGTTAAAGGAGTTGCTTT+AGG | 0.341836 | 7.3:-32961673 | MS.gene42114:CDS |
| AATTGGGAAAGTATGGTATA+AGG | 0.304183 | 7.3:-32961498 | MS.gene42114:CDS |
| ACAGGTGGAGCTAGAGGAAT+TGG | 0.380505 | 7.3:-32961985 | MS.gene42114:CDS |
| ACTTCAACACTTTCTCACTA+TGG | 0.383031 | 7.3:-32961805 | MS.gene42114:CDS |
| ACTTCCTCAAGAAATTGTAT+TGG | 0.410994 | 7.3:-32961211 | MS.gene42114:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAATAGCTATTGTAACAGG+TGG | - | chr7.3:32961651-32961670 | MS.gene42114:CDS | 30.0% | |
| AAAGAATGCTTCTTGTGAAT+TGG | - | chr7.3:32962136-32962155 | MS.gene42114:intron | 30.0% | |
| AAGAATGCTTCTTGTGAATT+GGG | - | chr7.3:32962137-32962156 | MS.gene42114:intron | 30.0% | |
| AATTGAGGAGGTTGAAAAAA+TGG | - | chr7.3:32962280-32962299 | MS.gene42114:intron | 30.0% | |
| ACTTCCTCAAGAAATTGTAT+TGG | - | chr7.3:32962440-32962459 | MS.gene42114:CDS | 30.0% | |
| CAATCCAATACAATTTCTTG+AGG | + | chr7.3:32962447-32962466 | None:intergenic | 30.0% | |
| GATGAATCTAAGTATGTTAG+TGG | - | chr7.3:32962389-32962408 | MS.gene42114:CDS | 30.0% | |
| GGAAAAATAGCTATTGTAAC+AGG | - | chr7.3:32961648-32961667 | MS.gene42114:CDS | 30.0% | |
| GTCATGAGTGTTAATGTTAA+AGG | - | chr7.3:32961966-32961985 | MS.gene42114:CDS | 30.0% | |
| GTTAATTGCATTTCTCCATT+TGG | - | chr7.3:32962176-32962195 | MS.gene42114:intron | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 32961204 | 32962469 | 32961204 | ID=MS.gene42114 |
| chr7.3 | mRNA | 32961204 | 32962469 | 32961204 | ID=MS.gene42114.t1;Parent=MS.gene42114 |
| chr7.3 | exon | 32962387 | 32962469 | 32962387 | ID=MS.gene42114.t1.exon1;Parent=MS.gene42114.t1 |
| chr7.3 | CDS | 32962387 | 32962469 | 32962387 | ID=cds.MS.gene42114.t1;Parent=MS.gene42114.t1 |
| chr7.3 | exon | 32961204 | 32962032 | 32961204 | ID=MS.gene42114.t1.exon2;Parent=MS.gene42114.t1 |
| chr7.3 | CDS | 32961204 | 32962032 | 32961204 | ID=cds.MS.gene42114.t1;Parent=MS.gene42114.t1 |
| Gene Sequence |
>MS.gene42114 ATGGGTGTAATGTCTGAGAAACCCCTTCAAGAACTTTCTCTTCACTCTACAGACACCACATTTTCTCCTCCTTCACCTAAGAGGTACTAAAATTTCTATATCCAAAGTAATATAATTAATGTCTTTTTTTTTTTTGTGGTGGACGGGGTTTGAATCTCAGACCTTTTGCATATATTATGCATTGTCCCTACCAACTGAGTTAAACTCATGAGAACATTAATGTCTTTTTCTATTTACCTTGAATTATTATTTTATTATTATTATTATTATTATCATCATCATCATCATCATTATTATTATTTTTACTATGTTTTGGTTTACCATGAAAAATTTCATATTGGATAAGTATATGTCTCTTCCACCCCATCAATTGATAGTAATCTTCAATCTTGTTAACTAACTACGAATCTTATGGAATATTTGATATTGTTATATAGGTTGGAAGGAAAAATAGCTATTGTAACAGGTGGAGCTAGAGGAATTGGAGAAGCAACGGTGAGAATTTTTGTGAAACATGGTGCAAAAGTGGTAATTGGTGATGTTGAAGATGAACTTGGAATCATGCTTGCAAATTCACTCTCTCCATCAGCTATTTATGTCCATTGTGATGTTAGTGTTGAAAAAGATGTTGAAAATTTAGTCACTTCAACACTTTCTCACTATGGAAAACTTGACATAATGTTCAACAATGCTGGCTTTCTTGGAAACCAATCCAAGAACAAAAAAAGCATTGTGAACTTCGACGCCGAGGAATTCGACCGAGTCATGAGTGTTAATGTTAAAGGAGTTGCTTTAGGAATGAAACATGCTGCAAAGGTTATGATACCTAGAGGAATTGGATGCATAATTTCCACTTCTAGTGTAGCTGGAGTTTTAGGAGGACTTGGTCCACATGCTTATACAGCTTCAAAGCATGCTATAGTTGGATTAACAAAGAATGCTTCTTGTGAATTGGGAAAGTATGGTATAAGGGTTAATTGCATTTCTCCATTTGGTGTTGCAACATCTATGCTTGTGAATGCTTGGAGAAATGGAGATGATGAAGTTGATGAGGGAATCAATTTTGGATTGCCTTTAATTGAGGAGGTTGAAAAAATGGAGGAATTTGTTAGAGGAATTGGTAATTTGAGAGGAACAACTTTAAAGGCTCAAGATATTGCTGAAGCTGTTCTTTATCTTGCTAGTGATGAATCTAAGTATGTTAGTGGTCATAATCTAGTTGTTGATGGTGGAATTACTTCCTCAAGAAATTGTATTGGATTGTGA |
| Protein sequence |
>MS.gene42114.t1 MGVMSEKPLQELSLHSTDTTFSPPSPKRLEGKIAIVTGGARGIGEATVRIFVKHGAKVVIGDVEDELGIMLANSLSPSAIYVHCDVSVEKDVENLVTSTLSHYGKLDIMFNNAGFLGNQSKNKKSIVNFDAEEFDRVMSVNVKGVALGMKHAAKVMIPRGIGCIISTSSVAGVLGGLGPHAYTASKHAIVGLTKNASCELGKYGIRVNCISPFGVATSMLVNAWRNGDDEVDEGINFGLPLIEEVEKMEEFVRGIGNLRGTTLKAQDIAEAVLYLASDESKYVSGHNLVVDGGITSSRNCIGL |
