AlfalfaGEDB | MS.gene42856

M. sativa cultivar XinJiangDaYe / MS.gene42856

KEGG: -
GO: -
PFAM: PF13561.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene42856.t1	XP_003623493.1	97.7	303	6	1	1	303	1	302	1.20E-162	582.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene42856.t1	Q9SCU0	66.4	301	80	6	13	303	14	303	1.3e-104	380.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene42856.t1	G7KV46	97.7	303	6	1	1	303	1	302	8.7e-163	582.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene42260	MS.gene42856	PPI
MS.gene08694	MS.gene42856	PPI
MS.gene003926	MS.gene42856	PPI
MS.gene42856	MS.gene008162	PPI
MS.gene42856	MS.gene54598	PPI
MS.gene42856	MS.gene07097	PPI
MS.gene42856	MS.gene63482	PPI
MS.gene42856	MS.gene003925	PPI
MS.gene42856	MS.gene52742	PPI
MS.gene42856	MS.gene31507	PPI
MS.gene42856	MS.gene28847	PPI
MS.gene42856	MS.gene08695	PPI
MS.gene42856	MS.gene95020	PPI
MS.gene42856	MS.gene065380	PPI
MS.gene044694	MS.gene42856	PPI
MS.gene63484	MS.gene42856	PPI
MS.gene42856	MS.gene008161	PPI
MS.gene42856	MS.gene63483	PPI
MS.gene42856	MS.gene50522	PPI
MS.gene42856	MS.gene62187	PPI
MS.gene42856	MS.gene03319	PPI
MS.gene42856	MS.gene08694	PPI
MS.gene42856	MS.gene043387	PPI
MS.gene42856	MS.gene63403	PPI
MS.gene42856	MS.gene04700	PPI
MS.gene42856	MS.gene014425	PPI
MS.gene41122	MS.gene42856	PPI
MS.gene42856	MS.gene006999	PPI
MS.gene63308	MS.gene42856	PPI
MS.gene033685	MS.gene42856	PPI
MS.gene42856	MS.gene039828	PPI
MS.gene045581	MS.gene42856	PPI
MS.gene42856	MS.gene25667	PPI
MS.gene42856	MS.gene30551	PPI
MS.gene28848	MS.gene42856	PPI
MS.gene42856	MS.gene63306	PPI
MS.gene42856	MS.gene23019	PPI
MS.gene050431	MS.gene42856	PPI
MS.gene42856	MS.gene055846	PPI
MS.gene62193	MS.gene42856	PPI
MS.gene42856	MS.gene63300	PPI
MS.gene42856	MS.gene033685	PPI
MS.gene42856	MS.gene25674	PPI
MS.gene42856	MS.gene85724	PPI
MS.gene42856	MS.gene008153	PPI
MS.gene73088	MS.gene42856	PPI
MS.gene42856	MS.gene73824	PPI
MS.gene073684	MS.gene42856	PPI
MS.gene42856	MS.gene80360	PPI
MS.gene42856	MS.gene008160	PPI
MS.gene42856	MS.gene08756	PPI
MS.gene54952	MS.gene42856	PPI
MS.gene42856	MS.gene024269	PPI
MS.gene42856	MS.gene003729	PPI
MS.gene42856	MS.gene019786	PPI
MS.gene050432	MS.gene42856	PPI
MS.gene42856	MS.gene050131	PPI
MS.gene42856	MS.gene43481	PPI
MS.gene42856	MS.gene007678	PPI
MS.gene42856	MS.gene52743	PPI
MS.gene012794	MS.gene42856	PPI
MS.gene007006	MS.gene42856	PPI
MS.gene42856	MS.gene028938	PPI
MS.gene42856	MS.gene072664	PPI
MS.gene42856	MS.gene003992	PPI
MS.gene42856	MS.gene021164	PPI
MS.gene42856	MS.gene031693	PPI
MS.gene62194	MS.gene42856	PPI
MS.gene42856	MS.gene62195	PPI
MS.gene63307	MS.gene42856	PPI
MS.gene006617	MS.gene42856	PPI
MS.gene42856	MS.gene07096	PPI
MS.gene42856	MS.gene064756	PPI
MS.gene42856	MS.gene018066	PPI
MS.gene056339	MS.gene42856	PPI
MS.gene42856	MS.gene50524	PPI
MS.gene42856	MS.gene007002	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene42856.t1	MTR_7g071680	97.690	303	6	1	1	303	1	302	0.0	599
MS.gene42856.t1	MTR_4g052340	52.920	274	105	5	27	299	12	262	2.49e-92	275
MS.gene42856.t1	MTR_4g052350	52.158	278	107	5	27	301	12	266	1.39e-90	271
MS.gene42856.t1	MTR_4g052300	51.087	276	111	4	25	299	4	256	4.76e-88	264
MS.gene42856.t1	MTR_4g052320	50.179	279	110	4	25	299	4	257	6.97e-86	258
MS.gene42856.t1	MTR_7g107670	44.138	290	147	2	10	299	14	288	2.86e-85	258
MS.gene42856.t1	MTR_7g083870	49.084	273	125	4	27	299	33	291	1.48e-82	251
MS.gene42856.t1	MTR_3g020670	50.519	289	126	5	16	301	2	276	1.95e-81	248
MS.gene42856.t1	MTR_2g090165	50.357	280	114	5	25	301	2	259	4.36e-81	246
MS.gene42856.t1	MTR_7g105460	49.814	269	114	4	28	295	12	260	6.42e-81	246
MS.gene42856.t1	MTR_7g110130	49.462	279	119	4	25	301	2	260	5.10e-79	241
MS.gene42856.t1	MTR_7g110140	48.540	274	118	4	28	299	5	257	2.75e-77	236
MS.gene42856.t1	MTR_4g078630	46.263	281	133	6	25	303	15	279	2.61e-76	235
MS.gene42856.t1	MTR_7g105580	46.324	272	107	3	27	297	6	239	3.60e-74	228
MS.gene42856.t1	MTR_7g105590	58.824	187	73	3	27	212	6	189	6.92e-72	220
MS.gene42856.t1	MTR_7g110160	47.985	273	101	5	25	295	2	235	2.39e-69	216
MS.gene42856.t1	MTR_6g023590	44.444	270	127	5	28	294	12	261	1.52e-68	214
MS.gene42856.t1	MTR_6g035395	44.891	274	117	4	27	299	3	243	3.00e-67	210
MS.gene42856.t1	MTR_6g023910	45.588	272	125	7	24	292	8	259	5.31e-67	211
MS.gene42856.t1	MTR_6g023990	42.491	273	134	6	25	294	10	262	1.16e-64	204
MS.gene42856.t1	MTR_6g023610	43.796	274	131	6	22	292	6	259	5.53e-64	202
MS.gene42856.t1	MTR_6g023950	41.837	294	135	8	4	294	1	261	4.97e-63	200
MS.gene42856.t1	MTR_6g023600	41.852	270	134	5	28	294	12	261	4.50e-61	195
MS.gene42856.t1	MTR_6g023600	41.111	270	130	6	28	294	12	255	2.18e-56	183
MS.gene42856.t1	MTR_3g113870	40.455	220	116	2	37	256	29	233	9.75e-56	180
MS.gene42856.t1	MTR_7g105570	50.000	198	79	2	98	295	40	217	1.73e-55	179
MS.gene42856.t1	MTR_4g084800	54.887	133	56	2	27	158	11	140	3.45e-44	148
MS.gene42856.t1	MTR_3g068155	33.091	275	147	7	29	295	19	264	6.03e-29	112
MS.gene42856.t1	MTR_3g085740	29.268	287	153	7	22	294	68	318	8.54e-28	110
MS.gene42856.t1	MTR_3g068175	31.618	272	155	8	29	295	17	262	9.63e-28	108
MS.gene42856.t1	MTR_3g068070	31.159	276	151	8	29	295	15	260	4.65e-27	107
MS.gene42856.t1	MTR_7g094520	33.569	283	148	10	23	294	6	259	5.58e-26	103
MS.gene42856.t1	MTR_3g068085	31.408	277	149	10	29	295	15	260	7.63e-26	103
MS.gene42856.t1	MTR_3g068175	31.159	276	155	9	29	295	17	266	1.45e-25	103
MS.gene42856.t1	MTR_4g064883	30.996	271	158	7	29	295	14	259	1.78e-25	102
MS.gene42856.t1	MTR_7g094510	32.482	274	150	9	29	293	6	253	2.88e-25	102
MS.gene42856.t1	MTR_4g132850	32.130	277	152	11	26	296	4	250	3.15e-24	99.0
MS.gene42856.t1	MTR_4g064883	34.804	204	123	5	22	220	6	204	8.55e-23	94.4
MS.gene42856.t1	MTR_7g113650	27.723	303	173	10	7	293	16	288	2.49e-22	94.7
MS.gene42856.t1	MTR_3g068065	28.777	278	159	8	29	297	17	264	2.80e-22	94.4
MS.gene42856.t1	MTR_1g082760	28.214	280	157	9	28	293	39	288	3.39e-21	91.7
MS.gene42856.t1	MTR_8g442750	30.137	292	166	8	19	298	4	269	4.49e-21	91.3
MS.gene42856.t1	MTR_1196s0010	29.520	271	157	9	27	293	272	512	1.12e-20	92.4
MS.gene42856.t1	MTR_4g064887	27.055	292	174	8	20	303	6	266	1.42e-19	86.7
MS.gene42856.t1	MTR_4g131740	27.240	279	164	8	28	294	39	290	2.93e-19	86.3
MS.gene42856.t1	MTR_7g063330	28.309	272	169	6	29	296	15	264	2.94e-19	85.9
MS.gene42856.t1	MTR_7g063270	30.314	287	168	10	18	296	3	265	6.26e-19	84.7
MS.gene42856.t1	MTR_4g058840	29.412	289	158	11	26	295	2	263	1.06e-18	84.3
MS.gene42856.t1	MTR_4g058820	29.412	289	158	11	26	295	2	263	1.06e-18	84.3
MS.gene42856.t1	MTR_1g051165	28.975	283	164	10	27	295	12	271	1.65e-18	84.0
MS.gene42856.t1	MTR_7g063320	29.592	294	168	9	9	294	1	263	2.62e-18	83.2
MS.gene42856.t1	MTR_4g058810	29.710	276	162	9	29	295	11	263	8.80e-18	81.6
MS.gene42856.t1	MTR_1g068955	31.047	277	155	8	26	292	11	261	2.16e-17	80.9
MS.gene42856.t1	MTR_4g064887	30.392	204	133	4	20	219	6	204	3.17e-17	79.3
MS.gene42856.t1	MTR_7g063260	29.371	286	173	9	18	296	3	266	1.39e-15	75.5
MS.gene42856.t1	MTR_7g063390	27.106	273	170	6	29	295	16	265	1.85e-15	75.1
MS.gene42856.t1	MTR_7g063360	29.044	272	166	9	29	295	16	265	3.77e-14	71.6
MS.gene42856.t1	MTR_8g096580	28.500	200	134	5	29	224	45	239	1.14e-13	70.9
MS.gene42856.t1	MTR_7g105560	54.237	59	25	1	22	78	4	62	2.96e-13	66.6
MS.gene42856.t1	MTR_4g134660	30.693	202	125	8	27	220	4	198	1.02e-12	67.0
MS.gene42856.t1	MTR_4g066050	29.353	201	129	5	32	224	27	222	1.19e-12	67.0
MS.gene42856.t1	MTR_8g044240	29.596	223	133	8	12	222	59	269	4.50e-12	66.2
MS.gene42856.t1	MTR_4g097510	25.131	191	137	3	30	219	3	188	1.35e-11	64.3
MS.gene42856.t1	MTR_4g086400	29.756	205	116	5	34	219	9	204	1.42e-11	65.5
MS.gene42856.t1	MTR_7g080410	27.619	210	137	6	19	218	26	230	2.62e-11	63.5
MS.gene42856.t1	MTR_7g080410	27.619	210	137	6	19	218	26	230	2.62e-11	63.5
MS.gene42856.t1	MTR_4g058830	32.203	177	98	6	122	295	52	209	8.47e-11	61.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene42856.t1	AT3G51680	66.221	299	84	5	13	303	14	303	6.83e-132	377
MS.gene42856.t1	AT3G26770	48.897	272	125	2	27	298	39	296	1.12e-85	259
MS.gene42856.t1	AT3G29250	49.451	273	115	5	25	295	2	253	2.36e-81	247
MS.gene42856.t1	AT3G29250	46.980	298	127	6	8	295	13	289	2.37e-81	248
MS.gene42856.t1	AT2G47130	50.000	270	111	5	28	295	5	252	1.11e-80	245
MS.gene42856.t1	AT3G29250	46.333	300	128	6	8	295	13	291	8.53e-80	244
MS.gene42856.t1	AT3G26760	43.003	293	148	4	7	299	21	294	1.69e-79	243
MS.gene42856.t1	AT4G03140	45.588	272	133	2	27	298	76	332	2.27e-79	244
MS.gene42856.t1	AT2G47140	47.985	273	119	4	25	295	2	253	1.24e-78	240
MS.gene42856.t1	AT1G52340	45.683	278	132	3	27	299	16	279	3.08e-73	227
MS.gene42856.t1	AT3G29260	45.421	273	125	5	25	295	2	252	6.82e-73	225
MS.gene42856.t1	AT2G47120	46.667	270	119	6	28	295	6	252	4.76e-72	223
MS.gene42856.t1	AT2G47120	46.667	270	119	6	28	295	5	251	4.82e-72	223
MS.gene42856.t1	AT3G42960	47.842	278	122	7	25	297	4	263	3.24e-65	206
MS.gene42856.t1	AT5G06060	31.387	274	153	7	29	295	9	254	1.93e-30	115
MS.gene42856.t1	AT1G24360	28.428	299	177	8	5	294	47	317	1.77e-25	103
MS.gene42856.t1	AT5G18210	31.786	280	149	7	29	294	8	259	3.05e-24	99.4
MS.gene42856.t1	AT1G63380	30.897	301	162	12	12	295	1	272	5.79e-24	99.0
MS.gene42856.t1	AT1G63380	31.449	283	149	11	29	295	15	268	3.67e-23	96.7
MS.gene42856.t1	AT1G63380	31.449	283	149	11	29	295	17	270	4.42e-23	96.7
MS.gene42856.t1	AT4G05530	32.500	280	147	12	26	296	6	252	4.84e-23	95.9
MS.gene42856.t1	AT1G63380	31.095	283	150	11	29	295	22	275	5.33e-23	96.7
MS.gene42856.t1	AT1G62610	30.405	296	170	11	12	295	1	272	6.99e-23	96.3
MS.gene42856.t1	AT2G29260	31.618	272	155	8	29	295	68	313	9.18e-23	96.7
MS.gene42856.t1	AT3G55290	30.851	282	151	13	29	295	18	270	1.10e-22	95.5
MS.gene42856.t1	AT3G55290	30.435	299	163	14	12	295	1	269	1.15e-22	95.5
MS.gene42856.t1	AT1G62610	30.935	278	157	10	29	295	14	267	3.83e-22	94.0
MS.gene42856.t1	AT1G62610	30.935	278	157	10	29	295	13	266	4.21e-22	94.0
MS.gene42856.t1	AT1G62610	30.935	278	157	10	29	295	15	268	4.31e-22	94.0
MS.gene42856.t1	AT1G07440	29.197	274	158	8	29	295	12	256	5.05e-22	93.6
MS.gene42856.t1	AT3G04000	31.525	295	152	10	19	293	5	269	5.28e-22	93.6
MS.gene42856.t1	AT3G03980	29.866	298	163	10	12	293	1	268	8.05e-22	92.8
MS.gene42856.t1	AT5G18210	34.951	206	117	5	29	222	8	208	1.58e-21	92.4
MS.gene42856.t1	AT1G07450	28.676	272	162	7	29	295	8	252	2.02e-21	91.7
MS.gene42856.t1	AT2G29290	29.286	280	151	8	29	295	7	252	2.87e-21	91.3
MS.gene42856.t1	AT2G29330	26.937	271	169	6	29	295	7	252	3.72e-21	90.9
MS.gene42856.t1	AT2G17845	30.142	282	152	11	29	293	47	300	5.95e-21	91.3
MS.gene42856.t1	AT3G46170	30.249	281	154	12	29	295	26	278	8.14e-21	90.5
MS.gene42856.t1	AT4G13180	32.000	275	152	8	29	294	13	261	1.25e-20	89.7
MS.gene42856.t1	AT2G29310	28.467	274	161	7	29	295	7	252	1.35e-20	89.4
MS.gene42856.t1	AT2G29340	27.737	274	163	7	29	295	7	252	2.24e-20	89.0
MS.gene42856.t1	AT2G30670	26.568	271	170	5	29	295	7	252	2.97e-20	88.6
MS.gene42856.t1	AT2G29340	27.737	274	163	7	29	295	7	252	3.49e-20	89.0
MS.gene42856.t1	AT3G12800	29.932	294	159	8	22	298	3	266	4.68e-20	88.6
MS.gene42856.t1	AT2G29320	29.749	279	152	9	29	295	13	259	9.31e-20	87.4
MS.gene42856.t1	AT3G55310	30.526	285	148	14	29	295	17	269	9.45e-20	87.4
MS.gene42856.t1	AT1G54870	25.676	296	176	10	11	293	66	330	1.43e-19	87.8
MS.gene42856.t1	AT1G54870	25.676	296	176	10	11	293	68	332	1.58e-19	87.8
MS.gene42856.t1	AT3G05260	26.071	280	164	7	28	294	36	285	2.64e-19	86.3
MS.gene42856.t1	AT2G29300	27.076	277	162	9	29	295	7	253	3.06e-19	85.9
MS.gene42856.t1	AT1G10310	31.905	210	136	3	19	226	5	209	6.49e-19	84.3
MS.gene42856.t1	AT2G29300	26.855	283	167	9	29	301	7	259	6.52e-19	85.1
MS.gene42856.t1	AT2G29370	28.520	277	164	8	26	295	12	261	7.67e-19	84.7
MS.gene42856.t1	AT2G29360	27.273	275	163	6	29	295	16	261	8.45e-19	84.7
MS.gene42856.t1	AT2G29350	29.304	273	159	7	29	295	15	259	1.38e-18	84.0
MS.gene42856.t1	AT2G29310	32.828	198	118	5	29	219	7	196	3.38e-18	81.6
MS.gene42856.t1	AT2G29340	31.937	191	115	5	29	212	7	189	1.23e-17	80.1
MS.gene42856.t1	AT2G29340	31.937	191	115	5	29	212	7	189	1.23e-17	80.1
MS.gene42856.t1	AT2G29290	29.439	214	123	6	84	295	48	235	1.13e-16	78.2
MS.gene42856.t1	AT2G29320	33.333	198	116	6	29	218	13	202	3.23e-16	76.3
MS.gene42856.t1	AT2G29350	31.472	197	122	4	29	219	15	204	2.67e-15	73.9
MS.gene42856.t1	AT2G29310	26.459	257	154	7	46	295	22	250	5.36e-14	70.9
MS.gene42856.t1	AT2G29350	28.638	213	125	5	84	295	35	221	8.51e-14	70.1
MS.gene42856.t1	AT5G50600	26.906	223	137	6	21	231	37	245	1.87e-13	70.5
MS.gene42856.t1	AT5G50700	26.906	223	137	6	21	231	37	245	1.87e-13	70.5
MS.gene42856.t1	AT2G29320	27.863	262	145	9	46	295	12	241	2.55e-13	68.9
MS.gene42856.t1	AT5G04900	30.457	197	125	4	35	222	83	276	5.88e-12	65.9
MS.gene42856.t1	AT5G50690	30.387	181	111	5	27	200	43	215	1.44e-11	64.3
MS.gene42856.t1	AT5G50590	30.387	181	111	5	27	200	43	215	1.44e-11	64.3
MS.gene42856.t1	AT5G50690	30.387	181	111	5	27	200	43	215	1.65e-11	63.5
MS.gene42856.t1	AT5G50590	30.387	181	111	5	27	200	43	215	1.65e-11	63.5
MS.gene42856.t1	AT2G29310	30.387	181	111	5	46	219	22	194	2.37e-11	62.4
MS.gene42856.t1	AT3G03330	28.646	192	125	6	29	212	41	228	3.21e-11	63.5
MS.gene42856.t1	AT1G07440	31.902	163	100	5	29	186	12	168	9.74e-11	60.1

Find 58 sgRNAs with CRISPR-Local

Find 96 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTCTCAACCTCCTCAATTAA+AGG	0.126621	7.2:+32178714	None:intergenic
GTTGATGAAGGAATCAATTT+TGG	0.164036	7.2:-32178741	MS.gene42856:CDS
TTCAACAATGCTGGCTTTCT+TGG	0.183584	7.2:-32179104	MS.gene42856:CDS
AATTGGGAAAATATGGTATA+AGG	0.231461	7.2:-32178836	MS.gene42856:CDS
TTTGAGAGGAACAACTTTAA+AGG	0.232093	7.2:-32178661	MS.gene42856:CDS
AAAGAATGCTTCTTGTGAAT+TGG	0.241146	7.2:-32178853	MS.gene42856:CDS
AATTGAGGAGGTTGAGAAAA+TGG	0.244589	7.2:-32178709	MS.gene42856:CDS
GTCATGAGTGTTAATGTTAA+AGG	0.281504	7.2:-32179023	MS.gene42856:CDS
TCTTGTGAATTGGGAAAATA+TGG	0.307659	7.2:-32178843	MS.gene42856:CDS
AATGTTAAAGGAGTTGCTTT+AGG	0.341836	7.2:-32179011	MS.gene42856:CDS
GTTAATTGCATTTCTCCATT+TGG	0.358113	7.2:-32178813	MS.gene42856:CDS
AAGAATGCTTCTTGTGAATT+GGG	0.359321	7.2:-32178852	MS.gene42856:CDS
CTTGTAAATGCTTGGAGAAA+TGG	0.366546	7.2:-32178774	MS.gene42856:CDS
GTTATGATACCTAGAGGAAT+TGG	0.379336	7.2:-32178969	MS.gene42856:CDS
ACAGGTGGAGCTAGAGGAAT+TGG	0.380505	7.2:-32179323	MS.gene42856:CDS
CATGGTGCAAAAGTGGTAAT+TGG	0.384110	7.2:-32179272	MS.gene42856:CDS
AAAATGGAGGAATTTGTTAG+AGG	0.396358	7.2:-32178693	MS.gene42856:CDS
GTGAAGAGAAAGTTCTTGAA+GGG	0.396504	7.2:+32179871	None:intergenic
GAGGAATTTGTTAGAGGGAT+TGG	0.408292	7.2:-32178687	MS.gene42856:CDS
GCTTCAAAGCATGCTATAGT+TGG	0.409180	7.2:-32178882	MS.gene42856:CDS
ACTTCCTCAAGAAATTGTAT+TGG	0.410994	7.2:-32178549	MS.gene42856:CDS
ACATCAACACTTTCTCACTA+TGG	0.431385	7.2:-32179143	MS.gene42856:CDS
ATTTCCACTTCTAGTGTAGC+TGG	0.432577	7.2:-32178939	MS.gene42856:CDS
GACATAATGTTCAACAATGC+TGG	0.445419	7.2:-32179113	MS.gene42856:CDS
GATGAATCTAAGTATGTTAG+TGG	0.446032	7.2:-32178600	MS.gene42856:CDS
ATTGGGAAAATATGGTATAA+GGG	0.447917	7.2:-32178835	MS.gene42856:CDS
TTTCTCCTCCTTCACCTAAG+AGG	0.457332	7.2:-32179832	MS.gene42856:intron
CATCTATGCTTGTAAATGCT+TGG	0.458090	7.2:-32178782	MS.gene42856:CDS
AAATGGAGGAATTTGTTAGA+GGG	0.462946	7.2:-32178692	MS.gene42856:CDS
AACTCCAGCTACACTAGAAG+TGG	0.492424	7.2:+32178935	None:intergenic
GGTCATAATCTAGTTGTTGA+TGG	0.504632	7.2:-32178579	MS.gene42856:CDS
ATAGATGTTGCAACACCAAA+TGG	0.508686	7.2:+32178798	None:intergenic
TGATATTGTTATAGGTTGGA+AGG	0.510402	7.2:-32179362	MS.gene42856:intron
AGTGAAGAGAAAGTTCTTGA+AGG	0.511591	7.2:+32179870	None:intergenic
GGTGATGTTGAAGATGAACT+TGG	0.516407	7.2:-32179251	MS.gene42856:CDS
TGAAGAGAAAGTTCTTGAAG+GGG	0.527961	7.2:+32179872	None:intergenic
GGAGAAGATGAAGTTGATGA+AGG	0.531073	7.2:-32178753	MS.gene42856:CDS
AGGAATGAAACATGCTGCAA+AGG	0.544045	7.2:-32178991	MS.gene42856:CDS
AGAGGGATTGGTAATTTGAG+AGG	0.546721	7.2:-32178675	MS.gene42856:CDS
TGGATTGCCTTTAATTGAGG+AGG	0.552107	7.2:-32178721	MS.gene42856:CDS
CAATGGACATAAATAGCTGA+TGG	0.565528	7.2:+32179203	None:intergenic
TGTGAAACATGGTGCAAAAG+TGG	0.566017	7.2:-32179279	MS.gene42856:CDS
CATGACTCGGTCGAATTCCT+CGG	0.569713	7.2:+32179040	None:intergenic
AAAATAGCTATTGTAACAGG+TGG	0.571177	7.2:-32179338	MS.gene42856:CDS
TAGTACCTCTTAGGTGAAGG+AGG	0.580775	7.2:+32179827	None:intergenic
ATTATGCATCCAATTCCTCT+AGG	0.581954	7.2:+32178960	None:intergenic
CAATCCAATACAATTTCTTG+AGG	0.587313	7.2:+32178545	None:intergenic
ATTGTAACAGGTGGAGCTAG+AGG	0.598201	7.2:-32179329	MS.gene42856:CDS
AGGTGAAGGAGGAGAAAATG+TGG	0.602352	7.2:+32179838	None:intergenic
CATTGTGAACTTCGACGCCG+AGG	0.604387	7.2:-32179057	MS.gene42856:CDS
TAACATTAACACTCATGACT+CGG	0.611584	7.2:+32179027	None:intergenic
TTTCAACACTAACATCACAA+TGG	0.616635	7.2:+32179186	None:intergenic
TAGAGGAATTGGAGAAGCAA+CGG	0.616718	7.2:-32179312	MS.gene42856:CDS
CATAATCTAGTTGTTGATGG+TGG	0.619135	7.2:-32178576	MS.gene42856:CDS
TGAGGAGGTTGAGAAAATGG+AGG	0.631267	7.2:-32178706	MS.gene42856:CDS
AAAGGCTCAAGATATTGCTG+AGG	0.652775	7.2:-32178643	MS.gene42856:CDS
GCAAAGGTTATGATACCTAG+AGG	0.690868	7.2:-32178975	MS.gene42856:CDS
TTTGAAGCTGTATAAGCATG+TGG	0.732644	7.2:+32178897	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTATTATTATTACTATGTTT+TGG	-	chr7.2:32178870-32178889	MS.gene42856:CDS	10.0%
!!!	TAATGTCTTTTTTTTTTGTT+TGG	-	chr7.2:32178658-32178677	MS.gene42856:CDS	15.0%
!!	GGAATATTTGATATTGTTAT+AGG	-	chr7.2:32179065-32179084	MS.gene42856:CDS	20.0%
!!	TATTTGATATTGTTATAGGT+TGG	-	chr7.2:32179069-32179088	MS.gene42856:CDS	20.0%
!!	TTAACTAACTATGAATCTTA+TGG	-	chr7.2:32179044-32179063	MS.gene42856:CDS	20.0%
!	AAGATTACTATCAATTGATG+GGG	+	chr7.2:32178941-32178960	None:intergenic	25.0%
!	AATCAATATGAGACCATATT+TGG	+	chr7.2:32178984-32179003	None:intergenic	25.0%
!	AATTGGGAAAATATGGTATA+AGG	-	chr7.2:32179599-32179618	MS.gene42856:intron	25.0%
!	ATTGGGAAAATATGGTATAA+GGG	-	chr7.2:32179600-32179619	MS.gene42856:intron	25.0%
!	GAAGATTACTATCAATTGAT+GGG	+	chr7.2:32178942-32178961	None:intergenic	25.0%
!	GCATAATATATGCAAGATTT+GGG	+	chr7.2:32178703-32178722	None:intergenic	25.0%
!	TACCATGAAAAATTTCATAC+TGG	-	chr7.2:32178895-32178914	MS.gene42856:CDS	25.0%
!	TGAAGATTACTATCAATTGA+TGG	+	chr7.2:32178943-32178962	None:intergenic	25.0%
!	TGCATAATATATGCAAGATT+TGG	+	chr7.2:32178704-32178723	None:intergenic	25.0%
!!	ATCCAGTATGAAATTTTTCA+TGG	+	chr7.2:32178900-32178919	None:intergenic	25.0%
!!!	TAGAAATTTTAGTACCTCTT+AGG	+	chr7.2:32178620-32178639	None:intergenic	25.0%
!!!	TGTCTTTTTTTTTTGTTTGG+TGG	-	chr7.2:32178661-32178680	MS.gene42856:CDS	25.0%
	AAAATAGCTATTGTAACAGG+TGG	-	chr7.2:32179097-32179116	MS.gene42856:CDS	30.0%
	AAAGAATGCTTCTTGTGAAT+TGG	-	chr7.2:32179582-32179601	MS.gene42856:intron	30.0%
	AAGAATGCTTCTTGTGAATT+GGG	-	chr7.2:32179583-32179602	MS.gene42856:intron	30.0%
	ACTTCCTCAAGAAATTGTAT+TGG	-	chr7.2:32179886-32179905	MS.gene42856:CDS	30.0%
	CAATCCAATACAATTTCTTG+AGG	+	chr7.2:32179893-32179912	None:intergenic	30.0%
	GATGAATCTAAGTATGTTAG+TGG	-	chr7.2:32179835-32179854	MS.gene42856:CDS	30.0%
	GGAAAAATAGCTATTGTAAC+AGG	-	chr7.2:32179094-32179113	MS.gene42856:CDS	30.0%
	GTCATGAGTGTTAATGTTAA+AGG	-	chr7.2:32179412-32179431	MS.gene42856:intron	30.0%
	GTTAATTGCATTTCTCCATT+TGG	-	chr7.2:32179622-32179641	MS.gene42856:intron	30.0%
	GTTATCCCAAAAACCAAATA+TGG	-	chr7.2:32178968-32178987	MS.gene42856:CDS	30.0%
	TAACATTAACACTCATGACT+CGG	+	chr7.2:32179411-32179430	None:intergenic	30.0%
	TCTTGTGAATTGGGAAAATA+TGG	-	chr7.2:32179592-32179611	MS.gene42856:intron	30.0%
	TTTCAACACTAACATCACAA+TGG	+	chr7.2:32179252-32179271	None:intergenic	30.0%
	TTTGAGAGGAACAACTTTAA+AGG	-	chr7.2:32179774-32179793	MS.gene42856:intron	30.0%
!	AATGTTAAAGGAGTTGCTTT+AGG	-	chr7.2:32179424-32179443	MS.gene42856:intron	30.0%
!	GTGAGAATTTTTGTGAAACA+TGG	-	chr7.2:32179145-32179164	MS.gene42856:CDS	30.0%
!	TGATATTGTTATAGGTTGGA+AGG	-	chr7.2:32179073-32179092	MS.gene42856:CDS	30.0%
!	TTTTGGATTGCCTTTAATTG+AGG	-	chr7.2:32179711-32179730	MS.gene42856:intron	30.0%
!!	AAAATGGAGGAATTTGTTAG+AGG	-	chr7.2:32179742-32179761	MS.gene42856:intron	30.0%
!!	AAATGGAGGAATTTGTTAGA+GGG	-	chr7.2:32179743-32179762	MS.gene42856:intron	30.0%
!!	GTTGATGAAGGAATCAATTT+TGG	-	chr7.2:32179694-32179713	MS.gene42856:intron	30.0%
!!!	ATGAGACCATATTTGGTTTT+TGG	+	chr7.2:32178977-32178996	None:intergenic	30.0%
!!!	CACAATGCTTTTTTTGTTCT+TGG	+	chr7.2:32179365-32179384	None:intergenic	30.0%
!!!	CTTTTTTTTTTGTTTGGTGG+TGG	-	chr7.2:32178664-32178683	MS.gene42856:CDS	30.0%
!!!	TGAGACCATATTTGGTTTTT+GGG	+	chr7.2:32178976-32178995	None:intergenic	30.0%
!!!	TGCTTTTTTTGTTCTTGGAT+TGG	+	chr7.2:32179360-32179379	None:intergenic	30.0%
	AATTGAGGAGGTTGAGAAAA+TGG	-	chr7.2:32179726-32179745	MS.gene42856:intron	35.0%
	ACATCAACACTTTCTCACTA+TGG	-	chr7.2:32179292-32179311	MS.gene42856:CDS	35.0%
	AGTGAAGAGAAAGTTCTTGA+AGG	+	chr7.2:32178568-32178587	None:intergenic	35.0%
	ATAGATGTTGCAACACCAAA+TGG	+	chr7.2:32179640-32179659	None:intergenic	35.0%
	ATTACTATCAATTGATGGGG+TGG	+	chr7.2:32178938-32178957	None:intergenic	35.0%
	ATTATGCATCCAATTCCTCT+AGG	+	chr7.2:32179478-32179497	None:intergenic	35.0%
	CAATGGACATAAATAGCTGA+TGG	+	chr7.2:32179235-32179254	None:intergenic	35.0%
	CATCTATGCTTGTAAATGCT+TGG	-	chr7.2:32179653-32179672	MS.gene42856:intron	35.0%
	CTTGTAAATGCTTGGAGAAA+TGG	-	chr7.2:32179661-32179680	MS.gene42856:intron	35.0%
	GACATAATGTTCAACAATGC+TGG	-	chr7.2:32179322-32179341	MS.gene42856:CDS	35.0%
	GGTCATAATCTAGTTGTTGA+TGG	-	chr7.2:32179856-32179875	MS.gene42856:CDS	35.0%
	GTGAAGAGAAAGTTCTTGAA+GGG	+	chr7.2:32178567-32178586	None:intergenic	35.0%
	GTTATGATACCTAGAGGAAT+TGG	-	chr7.2:32179466-32179485	MS.gene42856:intron	35.0%
	TGAAGAGAAAGTTCTTGAAG+GGG	+	chr7.2:32178566-32178585	None:intergenic	35.0%
	TTCTCAACCTCCTCAATTAA+AGG	+	chr7.2:32179724-32179743	None:intergenic	35.0%
!	CATAATCTAGTTGTTGATGG+TGG	-	chr7.2:32179859-32179878	MS.gene42856:CDS	35.0%
!	TGAGTTTAACTCAGTTGGTA+GGG	+	chr7.2:32178731-32178750	None:intergenic	35.0%
!	TTTGAAGCTGTATAAGCATG+TGG	+	chr7.2:32179541-32179560	None:intergenic	35.0%
!	TTTTAGTACCTCTTAGGTGA+AGG	+	chr7.2:32178614-32178633	None:intergenic	35.0%
	AAAGGCTCAAGATATTGCTG+AGG	-	chr7.2:32179792-32179811	MS.gene42856:intron	40.0%
	AGGAATGAAACATGCTGCAA+AGG	-	chr7.2:32179444-32179463	MS.gene42856:intron	40.0%
	ATTTCCACTTCTAGTGTAGC+TGG	-	chr7.2:32179496-32179515	MS.gene42856:intron	40.0%
	CATGGTGCAAAAGTGGTAAT+TGG	-	chr7.2:32179163-32179182	MS.gene42856:CDS	40.0%
	GCAAAGGTTATGATACCTAG+AGG	-	chr7.2:32179460-32179479	MS.gene42856:intron	40.0%
	GCTTCAAAGCATGCTATAGT+TGG	-	chr7.2:32179553-32179572	MS.gene42856:intron	40.0%
	GGTGATGTTGAAGATGAACT+TGG	-	chr7.2:32179184-32179203	MS.gene42856:CDS	40.0%
	GTGAGTTTAACTCAGTTGGT+AGG	+	chr7.2:32178732-32178751	None:intergenic	40.0%
	TAGAGGAATTGGAGAAGCAA+CGG	-	chr7.2:32179123-32179142	MS.gene42856:CDS	40.0%
	TGGATTGCCTTTAATTGAGG+AGG	-	chr7.2:32179714-32179733	MS.gene42856:intron	40.0%
	TGTGAAACATGGTGCAAAAG+TGG	-	chr7.2:32179156-32179175	MS.gene42856:CDS	40.0%
!	AAGATTTGGGATTCAAACCC+CGG	+	chr7.2:32178690-32178709	None:intergenic	40.0%
!	AGAGGGATTGGTAATTTGAG+AGG	-	chr7.2:32179760-32179779	MS.gene42856:intron	40.0%
!	TCTAGTGTAGCTGGAGTTTT+AGG	-	chr7.2:32179505-32179524	MS.gene42856:intron	40.0%
!!	GAGGAATTTGTTAGAGGGAT+TGG	-	chr7.2:32179748-32179767	MS.gene42856:intron	40.0%
!!	GGAGAAGATGAAGTTGATGA+AGG	-	chr7.2:32179682-32179701	MS.gene42856:intron	40.0%
!!	TTCAACAATGCTGGCTTTCT+TGG	-	chr7.2:32179331-32179350	MS.gene42856:CDS	40.0%
!!!	TTTTTTTGTTTGGTGGTGGC+CGG	-	chr7.2:32178668-32178687	MS.gene42856:CDS	40.0%
	AACTCCAGCTACACTAGAAG+TGG	+	chr7.2:32179503-32179522	None:intergenic	45.0%
	AGGTGAAGGAGGAGAAAATG+TGG	+	chr7.2:32178600-32178619	None:intergenic	45.0%
	ATTGTAACAGGTGGAGCTAG+AGG	-	chr7.2:32179106-32179125	MS.gene42856:CDS	45.0%
	CCAACTGAGTTAAACTCACG+AGG	-	chr7.2:32178733-32178752	MS.gene42856:CDS	45.0%
	CCTCGTGAGTTTAACTCAGT+TGG	+	chr7.2:32178736-32178755	None:intergenic	45.0%
	TAGTACCTCTTAGGTGAAGG+AGG	+	chr7.2:32178611-32178630	None:intergenic	45.0%
	TGAGGAGGTTGAGAAAATGG+AGG	-	chr7.2:32179729-32179748	MS.gene42856:intron	45.0%
	TTTCTCCTCCTTCACCTAAG+AGG	-	chr7.2:32178603-32178622	MS.gene42856:CDS	45.0%
!!!	AGTGTAGCTGGAGTTTTAGG+AGG	-	chr7.2:32179508-32179527	MS.gene42856:intron	45.0%
!!!	TTTTTTGTTTGGTGGTGGCC+GGG	-	chr7.2:32178669-32178688	MS.gene42856:CDS	45.0%
!!	ATAATAATAATAATAATTCA+AGG	+	chr7.2:32178782-32178801	None:intergenic	5.0%
	ACAGGTGGAGCTAGAGGAAT+TGG	-	chr7.2:32179112-32179131	MS.gene42856:CDS	50.0%
!!	CATGACTCGGTCGAATTCCT+CGG	+	chr7.2:32179398-32179417	None:intergenic	50.0%
!!!	GCTGGAGTTTTAGGAGGACT+TGG	-	chr7.2:32179514-32179533	MS.gene42856:intron	50.0%
!!!	TTTTTGTTTGGTGGTGGCCG+GGG	-	chr7.2:32178670-32178689	MS.gene42856:CDS	50.0%
	CATTGTGAACTTCGACGCCG+AGG	-	chr7.2:32179378-32179397	MS.gene42856:intron	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.2	gene	32178542	32179915	32178542	ID=MS.gene42856
chr7.2	mRNA	32178542	32179915	32178542	ID=MS.gene42856.t1;Parent=MS.gene42856
chr7.2	exon	32179833	32179915	32179833	ID=MS.gene42856.t1.exon1;Parent=MS.gene42856.t1
chr7.2	CDS	32179833	32179915	32179833	ID=cds.MS.gene42856.t1;Parent=MS.gene42856.t1
chr7.2	exon	32178542	32179370	32178542	ID=MS.gene42856.t1.exon2;Parent=MS.gene42856.t1
chr7.2	CDS	32178542	32179370	32178542	ID=cds.MS.gene42856.t1;Parent=MS.gene42856.t1

Gene Sequence

>MS.gene42856

ATGGGTGTAATGTCTGAGAAACCCCTTCAAGAACTTTCTCTTCACTCTACAGACACCACATTTTCTCCTCCTTCACCTAAGAGGTACTAAAATTTCTATATTCAAAGTAATATAATTAATGTCTTTTTTTTTTGTTTGGTGGTGGCCGGGGTTTGAATCCCAAATCTTGCATATATTATGCATTGTCCCTACCAACTGAGTTAAACTCACGAGGACATTAATGTCTTTTTCTATTTACCTTGAATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTACTATGTTTTGGTTTACCATGAAAAATTTCATACTGGATAAGTATATGTCTCTTCCACCCCATCAATTGATAGTAATCTTCAATCTTGTTATCCCAAAAACCAAATATGGTCTCATATTGATTATGCACATATATTGAGAAATGAATAGTGTTTTTTTAAAACTTAACTAACTATGAATCTTATGGAATATTTGATATTGTTATAGGTTGGAAGGAAAAATAGCTATTGTAACAGGTGGAGCTAGAGGAATTGGAGAAGCAACGGTGAGAATTTTTGTGAAACATGGTGCAAAAGTGGTAATTGGTGATGTTGAAGATGAACTTGGAATCATGCTTGCAAATTCACTCTCTCCATCAGCTATTTATGTCCATTGTGATGTTAGTGTTGAAAAAGATGTTGAAAATTTAGTCACATCAACACTTTCTCACTATGGAAAACTTGACATAATGTTCAACAATGCTGGCTTTCTTGGAAACCAATCCAAGAACAAAAAAAGCATTGTGAACTTCGACGCCGAGGAATTCGACCGAGTCATGAGTGTTAATGTTAAAGGAGTTGCTTTAGGAATGAAACATGCTGCAAAGGTTATGATACCTAGAGGAATTGGATGCATAATTTCCACTTCTAGTGTAGCTGGAGTTTTAGGAGGACTTGGTCCACATGCTTATACAGCTTCAAAGCATGCTATAGTTGGATTAACAAAGAATGCTTCTTGTGAATTGGGAAAATATGGTATAAGGGTTAATTGCATTTCTCCATTTGGTGTTGCAACATCTATGCTTGTAAATGCTTGGAGAAATGGAGAAGATGAAGTTGATGAAGGAATCAATTTTGGATTGCCTTTAATTGAGGAGGTTGAGAAAATGGAGGAATTTGTTAGAGGGATTGGTAATTTGAGAGGAACAACTTTAAAGGCTCAAGATATTGCTGAGGCTGTTCTTTATCTTGCTAGTGATGAATCTAAGTATGTTAGTGGTCATAATCTAGTTGTTGATGGTGGAATTACTTCCTCAAGAAATTGTATTGGATTGTGA

Protein sequence

>MS.gene42856.t1

MGVMSEKPLQELSLHSTDTTFSPPSPKRLEGKIAIVTGGARGIGEATVRIFVKHGAKVVIGDVEDELGIMLANSLSPSAIYVHCDVSVEKDVENLVTSTLSHYGKLDIMFNNAGFLGNQSKNKKSIVNFDAEEFDRVMSVNVKGVALGMKHAAKVMIPRGIGCIISTSSVAGVLGGLGPHAYTASKHAIVGLTKNASCELGKYGIRVNCISPFGVATSMLVNAWRNGEDEVDEGINFGLPLIEEVEKMEEFVRGIGNLRGTTLKAQDIAEAVLYLASDESKYVSGHNLVVDGGITSSRNCIGL