AlfalfaGEDB | MS.gene52743

M. sativa cultivar XinJiangDaYe / MS.gene52743

KEGG: K00059
GO: -
PFAM: PF13561.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene52743.t1	XP_013449699.1	95	260	13	0	1	260	1	260	2.80E-131	478

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene52743.t1	Q9SQR4	66.5	266	81	2	3	260	5	270	9.7e-93	341.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene52743.t1	I3SQZ5	95.0	260	13	0	1	260	1	260	2.0e-131	478.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene63483	MS.gene52743	PPI
MS.gene045581	MS.gene52743	PPI
MS.gene050431	MS.gene52743	PPI
MS.gene63403	MS.gene52743	PPI
MS.gene003925	MS.gene52743	PPI
MS.gene62193	MS.gene52743	PPI
MS.gene85724	MS.gene52743	PPI
MS.gene52743	MS.gene63482	PPI
MS.gene41122	MS.gene52743	PPI
MS.gene52743	MS.gene043387	PPI
MS.gene52743	MS.gene31507	PPI
MS.gene52743	MS.gene014425	PPI
MS.gene52743	MS.gene28847	PPI
MS.gene52743	MS.gene08695	PPI
MS.gene073684	MS.gene52743	PPI
MS.gene63308	MS.gene52743	PPI
MS.gene52743	MS.gene042142	PPI
MS.gene52743	MS.gene008161	PPI
MS.gene033685	MS.gene52743	PPI
MS.gene52743	MS.gene006999	PPI
MS.gene012794	MS.gene52743	PPI
MS.gene28848	MS.gene52743	PPI
MS.gene87026	MS.gene52743	PPI
MS.gene68956	MS.gene52743	PPI
MS.gene52743	MS.gene30551	PPI
MS.gene52743	MS.gene23019	PPI
MS.gene52743	MS.gene03319	PPI
MS.gene52743	MS.gene63300	PPI
MS.gene52743	MS.gene25674	PPI
MS.gene52743	MS.gene87780	PPI
MS.gene52743	MS.gene68880	PPI
MS.gene52743	MS.gene008153	PPI
MS.gene056339	MS.gene52743	PPI
MS.gene07096	MS.gene52743	PPI
MS.gene019786	MS.gene52743	PPI
MS.gene54952	MS.gene52743	PPI
MS.gene031693	MS.gene52743	PPI
MS.gene52743	MS.gene80360	PPI
MS.gene050432	MS.gene52743	PPI
MS.gene52743	MS.gene008160	PPI
MS.gene52743	MS.gene08756	PPI
MS.gene52743	MS.gene42114	PPI
MS.gene42260	MS.gene52743	PPI
MS.gene08694	MS.gene52743	PPI
MS.gene003926	MS.gene52743	PPI
MS.gene52743	MS.gene007678	PPI
MS.gene007006	MS.gene52743	PPI
MS.gene52743	MS.gene028938	PPI
MS.gene52743	MS.gene25667	PPI
MS.gene28847	MS.gene52743	PPI
MS.gene62194	MS.gene52743	PPI
MS.gene52743	MS.gene003992	PPI
MS.gene63307	MS.gene52743	PPI
MS.gene52743	MS.gene055846	PPI
MS.gene52743	MS.gene62195	PPI
MS.gene006617	MS.gene52743	PPI
MS.gene63484	MS.gene52743	PPI
MS.gene012681	MS.gene52743	PPI
MS.gene008162	MS.gene52743	PPI
MS.gene52743	MS.gene85724	PPI
MS.gene52743	MS.gene73824	PPI
MS.gene52743	MS.gene024269	PPI
MS.gene52743	MS.gene003729	PPI
MS.gene52743	MS.gene019786	PPI
MS.gene52743	MS.gene007002	PPI
MS.gene064756	MS.gene52743	PPI
MS.gene42856	MS.gene52743	PPI
MS.gene52743	MS.gene050131	PPI
MS.gene52743	MS.gene43481	PPI
MS.gene52743	MS.gene54598	PPI
MS.gene52743	MS.gene072664	PPI
MS.gene52743	MS.gene07097	PPI
MS.gene63306	MS.gene52743	PPI
MS.gene62187	MS.gene52743	PPI
MS.gene52743	MS.gene95020	PPI
MS.gene52743	MS.gene065380	PPI
MS.gene021164	MS.gene52743	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene52743.t1	MTR_7g094520	95.000	260	13	0	1	260	1	260	0.0	502
MS.gene52743.t1	MTR_7g094510	83.399	253	42	0	8	260	3	255	2.57e-154	430
MS.gene52743.t1	MTR_1g068955	67.871	249	79	1	10	257	13	261	6.05e-119	341
MS.gene52743.t1	MTR_3g085740	35.887	248	150	6	15	260	79	319	9.69e-37	132
MS.gene52743.t1	MTR_3g068070	32.946	258	151	10	11	259	15	259	1.35e-28	110
MS.gene52743.t1	MTR_3g068085	32.422	256	155	9	11	259	15	259	1.19e-27	107
MS.gene52743.t1	MTR_6g023610	31.061	264	172	5	2	260	4	262	1.62e-27	107
MS.gene52743.t1	MTR_6g023600	30.859	256	163	6	11	259	13	261	3.14e-27	106
MS.gene52743.t1	MTR_7g071680	32.624	282	151	9	6	259	23	293	3.54e-27	107
MS.gene52743.t1	MTR_6g023590	31.496	254	164	5	11	259	13	261	3.83e-27	105
MS.gene52743.t1	MTR_3g068065	32.812	256	154	9	11	259	17	261	8.40e-27	105
MS.gene52743.t1	MTR_7g063320	31.538	260	166	5	7	260	11	264	9.22e-27	105
MS.gene52743.t1	MTR_6g023600	30.859	256	157	6	11	259	13	255	1.03e-26	104
MS.gene52743.t1	MTR_7g063270	30.980	255	163	5	11	259	16	263	1.82e-26	104
MS.gene52743.t1	MTR_3g068175	32.558	258	152	11	11	259	17	261	2.65e-26	103
MS.gene52743.t1	MTR_1196s0010	32.669	251	154	6	13	260	276	514	2.98e-26	107
MS.gene52743.t1	MTR_6g023910	30.620	258	169	5	8	260	10	262	2.98e-26	103
MS.gene52743.t1	MTR_7g063260	31.641	256	161	5	11	259	16	264	4.22e-26	103
MS.gene52743.t1	MTR_7g063390	30.268	261	166	4	7	259	12	264	2.03e-25	101
MS.gene52743.t1	MTR_7g063360	29.885	261	167	4	7	259	12	264	2.40e-25	101
MS.gene52743.t1	MTR_4g064887	30.980	255	160	7	11	259	15	259	2.12e-24	98.6
MS.gene52743.t1	MTR_3g068155	31.395	258	163	8	7	259	15	263	3.35e-24	98.6
MS.gene52743.t1	MTR_6g023990	30.859	256	163	7	11	259	14	262	3.42e-24	98.2
MS.gene52743.t1	MTR_3g068175	32.061	262	152	12	11	259	17	265	3.81e-24	98.2
MS.gene52743.t1	MTR_4g132850	30.556	252	162	8	11	259	7	248	1.34e-22	93.6
MS.gene52743.t1	MTR_7g063330	28.405	257	175	4	7	259	11	262	7.72e-22	91.7
MS.gene52743.t1	MTR_6g023950	30.078	256	165	7	11	259	13	261	1.18e-21	91.3
MS.gene52743.t1	MTR_7g083870	30.337	267	153	9	11	259	35	286	1.59e-21	91.7
MS.gene52743.t1	MTR_7g107670	26.357	258	174	5	11	259	33	283	2.64e-21	90.9
MS.gene52743.t1	MTR_7g105460	30.566	265	172	5	1	259	1	259	3.92e-21	90.1
MS.gene52743.t1	MTR_4g131740	27.344	256	176	6	11	260	40	291	9.13e-21	89.4
MS.gene52743.t1	MTR_7g113650	28.016	257	172	6	11	260	40	290	1.57e-20	89.0
MS.gene52743.t1	MTR_3g020670	29.889	271	168	8	2	259	8	269	2.11e-20	88.2
MS.gene52743.t1	MTR_6g081270	67.797	59	19	0	39	97	35	93	2.97e-20	83.2
MS.gene52743.t1	MTR_4g064883	27.600	250	175	4	11	259	14	258	5.76e-20	86.7
MS.gene52743.t1	MTR_4g058810	29.167	264	178	7	1	258	1	261	7.41e-20	86.7
MS.gene52743.t1	MTR_7g105590	29.744	195	129	4	11	204	8	195	2.78e-19	83.6
MS.gene52743.t1	MTR_7g105580	27.559	254	154	5	11	259	8	236	1.44e-18	82.8
MS.gene52743.t1	MTR_7g080410	27.805	205	143	5	1	203	28	229	3.41e-18	82.4
MS.gene52743.t1	MTR_7g080410	27.805	205	143	5	1	203	28	229	3.67e-18	82.8
MS.gene52743.t1	MTR_4g058840	28.409	264	180	7	1	258	1	261	4.07e-18	82.0
MS.gene52743.t1	MTR_4g058820	28.409	264	180	7	1	258	1	261	4.07e-18	82.0
MS.gene52743.t1	MTR_4g052350	26.984	252	173	4	11	259	14	257	9.90e-18	80.9
MS.gene52743.t1	MTR_1g082760	27.059	255	177	6	11	260	40	290	2.50e-17	80.1
MS.gene52743.t1	MTR_1g051165	29.119	261	167	9	11	258	14	269	1.16e-16	77.8
MS.gene52743.t1	MTR_4g078630	27.099	262	168	6	11	259	19	270	2.47e-16	77.0
MS.gene52743.t1	MTR_7g063270	27.273	253	126	5	11	259	16	214	2.50e-16	75.9
MS.gene52743.t1	MTR_4g052300	28.405	257	161	5	11	258	8	250	9.50e-16	75.1
MS.gene52743.t1	MTR_4g052340	26.000	250	174	4	11	257	14	255	3.44e-15	73.6
MS.gene52743.t1	MTR_4g052320	26.459	257	169	4	11	259	8	252	3.95e-15	73.6
MS.gene52743.t1	MTR_8g442750	29.368	269	172	10	2	259	4	265	4.95e-15	73.6
MS.gene52743.t1	MTR_7g110140	28.516	256	167	8	11	259	6	252	5.04e-14	70.5
MS.gene52743.t1	MTR_7g110130	28.627	255	169	7	11	259	6	253	8.58e-14	69.7
MS.gene52743.t1	MTR_4g064887	31.156	199	120	8	11	203	15	202	1.03e-13	68.9
MS.gene52743.t1	MTR_7g105570	32.544	169	106	3	97	259	50	216	2.15e-13	68.2
MS.gene52743.t1	MTR_3g113870	26.633	199	134	5	4	198	11	201	1.11e-11	63.2
MS.gene52743.t1	MTR_2g090165	29.344	259	161	9	11	259	6	252	1.44e-11	63.5
MS.gene52743.t1	MTR_7g110160	25.882	255	157	6	11	259	6	234	2.75e-11	62.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene52743.t1	AT5G18210	68.441	263	77	2	1	260	1	260	3.29e-126	359
MS.gene52743.t1	AT3G03980	66.917	266	80	3	3	260	5	270	1.84e-124	355
MS.gene52743.t1	AT5G18210	68.952	248	71	2	1	245	1	245	1.32e-116	335
MS.gene52743.t1	AT3G04000	61.798	267	92	2	2	258	3	269	3.68e-115	331
MS.gene52743.t1	AT4G13180	59.684	253	102	0	8	260	10	262	4.30e-114	328
MS.gene52743.t1	AT1G24360	30.303	264	172	6	2	259	60	317	6.14e-30	114
MS.gene52743.t1	AT5G06060	29.365	252	168	6	11	259	9	253	1.42e-26	104
MS.gene52743.t1	AT3G51680	32.331	266	158	7	11	258	32	293	6.10e-26	103
MS.gene52743.t1	AT2G29290	30.279	251	167	5	11	259	7	251	1.01e-25	102
MS.gene52743.t1	AT2G30670	30.952	252	164	5	11	259	7	251	5.74e-25	100
MS.gene52743.t1	AT2G29150	29.804	255	160	7	11	258	16	258	2.35e-24	98.6
MS.gene52743.t1	AT3G26760	28.571	266	167	7	8	259	33	289	3.68e-24	99.0
MS.gene52743.t1	AT2G29360	29.675	246	165	5	16	259	21	260	1.83e-23	96.3
MS.gene52743.t1	AT1G52340	28.571	259	173	4	11	259	18	274	8.70e-23	94.7
MS.gene52743.t1	AT1G07440	30.000	260	163	9	7	259	8	255	1.08e-22	94.4
MS.gene52743.t1	AT2G47120	29.008	262	156	5	11	259	6	250	4.62e-22	92.4
MS.gene52743.t1	AT2G47120	29.008	262	156	5	11	259	7	251	4.86e-22	92.4
MS.gene52743.t1	AT2G29370	28.627	255	166	6	11	259	16	260	7.13e-22	92.0
MS.gene52743.t1	AT3G55310	29.434	265	175	8	3	258	6	267	7.13e-22	92.4
MS.gene52743.t1	AT2G29350	29.032	248	161	6	16	258	20	257	7.56e-22	92.0
MS.gene52743.t1	AT4G03140	27.132	258	172	5	11	259	78	328	2.30e-21	92.0
MS.gene52743.t1	AT3G46170	28.897	263	175	8	5	258	17	276	4.71e-21	90.1
MS.gene52743.t1	AT1G63380	28.947	266	176	8	3	258	6	268	4.90e-21	90.1
MS.gene52743.t1	AT1G62610	28.731	268	178	8	1	258	1	265	5.50e-21	89.7
MS.gene52743.t1	AT3G55290	28.346	254	173	7	11	258	17	267	6.96e-21	89.7
MS.gene52743.t1	AT3G55290	28.346	254	173	7	11	258	18	268	7.07e-21	89.7
MS.gene52743.t1	AT2G29260	26.531	245	174	4	16	259	73	312	8.08e-21	90.1
MS.gene52743.t1	AT1G63380	28.788	264	175	8	5	258	6	266	9.70e-21	89.4
MS.gene52743.t1	AT1G63380	28.788	264	175	8	5	258	13	273	9.85e-21	89.4
MS.gene52743.t1	AT2G17845	28.846	260	173	8	8	258	44	300	1.07e-20	89.7
MS.gene52743.t1	AT1G63380	28.788	264	175	8	5	258	10	270	1.26e-20	89.0
MS.gene52743.t1	AT1G62610	28.788	264	175	8	5	258	4	264	1.50e-20	88.6
MS.gene52743.t1	AT1G62610	28.788	264	175	8	5	258	6	266	1.63e-20	88.6
MS.gene52743.t1	AT1G62610	28.788	264	175	8	5	258	10	270	1.80e-20	88.6
MS.gene52743.t1	AT2G29290	31.122	196	130	4	66	259	42	234	3.20e-20	87.0
MS.gene52743.t1	AT2G29320	29.762	252	168	6	11	259	13	258	3.40e-20	87.4
MS.gene52743.t1	AT1G07450	27.734	256	166	7	11	259	8	251	4.88e-20	87.0
MS.gene52743.t1	AT4G05530	28.137	263	172	9	1	259	1	250	5.59e-20	86.7
MS.gene52743.t1	AT1G54870	27.734	256	174	6	11	260	82	332	5.71e-20	87.8
MS.gene52743.t1	AT2G29310	28.854	253	168	5	11	259	7	251	6.13e-20	86.7
MS.gene52743.t1	AT1G54870	27.734	256	174	6	11	260	84	334	6.45e-20	87.8
MS.gene52743.t1	AT2G29300	29.688	256	163	8	11	259	7	252	7.96e-20	86.3
MS.gene52743.t1	AT3G29250	30.588	255	163	6	11	259	6	252	1.20e-19	85.9
MS.gene52743.t1	AT2G29300	29.008	262	169	8	5	259	1	252	1.59e-19	85.9
MS.gene52743.t1	AT3G29250	30.588	255	163	6	11	259	44	290	1.88e-19	85.9
MS.gene52743.t1	AT3G29250	30.588	255	163	6	11	259	42	288	1.90e-19	85.9
MS.gene52743.t1	AT2G47130	30.078	256	162	8	11	259	6	251	7.66e-19	83.6
MS.gene52743.t1	AT2G29330	27.626	257	166	7	11	259	7	251	1.51e-18	82.8
MS.gene52743.t1	AT3G05260	26.562	256	176	6	11	260	37	286	1.59e-18	83.2
MS.gene52743.t1	AT3G29260	29.804	255	164	7	11	259	6	251	5.21e-18	81.3
MS.gene52743.t1	AT2G29340	29.183	257	162	9	11	259	7	251	5.67e-18	81.3
MS.gene52743.t1	AT2G29340	29.183	257	162	9	11	259	7	251	7.70e-18	81.6
MS.gene52743.t1	AT2G29350	27.692	195	135	2	66	258	29	219	3.71e-17	78.6
MS.gene52743.t1	AT3G03330	28.718	195	135	4	11	203	41	233	8.42e-16	76.3
MS.gene52743.t1	AT2G47140	29.412	255	166	7	11	259	6	252	1.42e-15	74.7
MS.gene52743.t1	AT3G26770	27.715	267	166	9	8	259	38	292	7.07e-15	73.2
MS.gene52743.t1	AT1G67730	32.821	195	121	6	16	205	54	243	1.53e-14	72.4
MS.gene52743.t1	AT2G29320	28.205	234	159	6	29	259	13	240	1.87e-14	71.2
MS.gene52743.t1	AT2G29310	27.234	235	159	5	29	259	23	249	3.78e-14	70.9
MS.gene52743.t1	AT3G42960	26.996	263	168	7	11	259	8	260	6.76e-13	67.4
MS.gene52743.t1	AT5G50600	28.774	212	135	8	8	212	42	244	9.64e-13	67.4
MS.gene52743.t1	AT5G50700	28.774	212	135	8	8	212	42	244	9.64e-13	67.4
MS.gene52743.t1	AT3G12800	28.626	262	165	8	11	259	10	262	1.02e-12	67.0
MS.gene52743.t1	AT3G01980	25.483	259	161	9	17	257	9	253	1.06e-12	66.6
MS.gene52743.t1	AT3G01980	25.483	259	161	9	17	257	9	253	1.06e-12	66.6
MS.gene52743.t1	AT2G29310	29.648	199	127	5	11	205	7	196	1.18e-12	65.5
MS.gene52743.t1	AT2G29320	31.795	195	125	6	11	203	13	201	5.41e-11	60.8

Find 76 sgRNAs with CRISPR-Local

Find 84 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGAAGCGATGACGAAGATTC+TGG	0.312867	7.2:+15126575	MS.gene52743:CDS
GTCTTGAAGTGGTGGTGATT+GGG	0.313321	7.2:-15126067	None:intergenic
AGTAATCCGTTTGGTAGAAT+TGG	0.346783	7.2:+15126711	MS.gene52743:CDS
TGGCATCTGATTCTGGTGAA+TGG	0.366032	7.2:+15126769	MS.gene52743:CDS
GCTGCGTTTAAGCCAGGTTA+TGG	0.372028	7.2:+15126525	MS.gene52743:CDS
GATTCTGGTGAATGGGTTAA+TGG	0.386598	7.2:+15126777	MS.gene52743:CDS
ACGGTTGTGTTTGCTATAGT+TGG	0.399470	7.2:-15126378	None:intergenic
GGTCTTGAAGTGGTGGTGAT+TGG	0.402875	7.2:-15126068	None:intergenic
GCGAAGGAGCTGAAGGGGAC+AGG	0.407465	7.2:+15126597	MS.gene52743:CDS
ACTGGCTCTTCTCGCGGAAT+CGG	0.423186	7.2:+15126105	MS.gene52743:CDS
AAAGATATTTCACCTGTTGT+TGG	0.427798	7.2:+15126741	MS.gene52743:CDS
AAGATATTTCACCTGTTGTT+GGG	0.433903	7.2:+15126742	MS.gene52743:CDS
GGCATCTGATTCTGGTGAAT+GGG	0.437775	7.2:+15126770	MS.gene52743:CDS
GATGTAAACCGGTGAGTTGA+AGG	0.438551	7.2:-15126319	None:intergenic
GCCGTGTACGCGCCATAACC+TGG	0.441582	7.2:-15126537	None:intergenic
ATCTCCGTCGCAGTCGGTCC+TGG	0.444828	7.2:-15126642	None:intergenic
AGATTAAAGCGTGGTGGTGG+AGG	0.460009	7.2:+15126471	MS.gene52743:CDS
CACGGCATCGAAAGCGGGAG+TGG	0.461544	7.2:+15126554	MS.gene52743:CDS
GGTCAAATTATTCGTGTCAA+TGG	0.464352	7.2:+15126798	MS.gene52743:CDS
GCGGGAACGGTGACGGCTCG+TGG	0.477779	7.2:-15126243	None:intergenic
ACTGCAAATTGCGTGGCGCC+AGG	0.477875	7.2:+15126624	MS.gene52743:CDS
ATTTCTGACAACTTCGTTAG+CGG	0.484072	7.2:+15126503	MS.gene52743:CDS
GCGTACACGGCATCGAAAGC+GGG	0.494837	7.2:+15126549	MS.gene52743:CDS
GGTTTGATTAGCGTTGATCT+CGG	0.499788	7.2:-15126217	None:intergenic
AGAGTCAGCGAGGTTGGAGT+TGG	0.504368	7.2:-15126187	None:intergenic
AGTGTAGTTGATGACGAGTC+TGG	0.504482	7.2:-15126163	None:intergenic
GACCGAGTTGCAATCGTCAC+TGG	0.504964	7.2:+15126087	MS.gene52743:CDS
ATTGCGGAAAGTAATCCGTT+TGG	0.508233	7.2:+15126702	MS.gene52743:CDS
CGAAGGAGCTGAAGGGGACA+GGG	0.514155	7.2:+15126598	MS.gene52743:CDS
GCGTTTCTCTGTGCAAAAGA+AGG	0.516086	7.2:+15126441	MS.gene52743:CDS
GTTTCACCAATTCTACCAAA+CGG	0.516345	7.2:-15126717	None:intergenic
ATCGGATACGTCGGCGGGAA+CGG	0.518820	7.2:-15126256	None:intergenic
TTAGCGGCTGCGTTTAAGCC+AGG	0.519829	7.2:+15126519	MS.gene52743:CDS
AACAGATTAAAGCGTGGTGG+TGG	0.523954	7.2:+15126468	MS.gene52743:CDS
GGACTCCGGCTGAGTTGACT+AGG	0.527746	7.2:-15126341	None:intergenic
ATCATAAACGTAAACGCAAG+AGG	0.529430	7.2:+15126417	MS.gene52743:CDS
GATTCTGGCGAAGGAGCTGA+AGG	0.530507	7.2:+15126590	MS.gene52743:CDS
AACGGTGACGGCTCGTGGAG+TGG	0.530987	7.2:-15126238	None:intergenic
TTTGCTATAGTTGGTAACTC+AGG	0.533251	7.2:-15126369	None:intergenic
TACGTCGGCGGGAACGGTGA+CGG	0.535562	7.2:-15126250	None:intergenic
TGATGGGAAAACGGAAGAGA+GGG	0.541968	7.2:+15126671	MS.gene52743:CDS
CGCGTACACGGCATCGAAAG+CGG	0.545539	7.2:+15126548	MS.gene52743:CDS
GGCGACAGAGTCAGCGAGGT+TGG	0.545641	7.2:-15126193	None:intergenic
ATCGTCACTGGCTCTTCTCG+CGG	0.549725	7.2:+15126099	MS.gene52743:CDS
TCTGGATCGGATACGTCGGC+GGG	0.550270	7.2:-15126261	None:intergenic
CAAATTATTCGTGTCAATGG+TGG	0.551650	7.2:+15126801	MS.gene52743:CDS
GAGTTGACTAGGATGTAAAC+CGG	0.567344	7.2:-15126330	None:intergenic
TCTTGAAGTGGTGGTGATTG+GGG	0.569944	7.2:-15126066	None:intergenic
TTCTGGCGAAGGAGCTGAAG+GGG	0.572191	7.2:+15126592	MS.gene52743:CDS
GATACGATCGAATGTTTCAA+CGG	0.574475	7.2:-15126397	None:intergenic
GATTAAAGCGTGGTGGTGGA+GGG	0.575333	7.2:+15126472	MS.gene52743:CDS
TACATCCTAGTCAACTCAGC+CGG	0.587669	7.2:+15126336	MS.gene52743:CDS
GCACTTCACCTTGCTTCACT+CGG	0.589568	7.2:+15126138	MS.gene52743:CDS
TGAACAAGCCTTCAACTCAC+CGG	0.591660	7.2:+15126311	MS.gene52743:CDS
GTCTGGCGCCGAGTGAAGCA+AGG	0.591721	7.2:-15126146	None:intergenic
TTCTGGATCGGATACGTCGG+CGG	0.592427	7.2:-15126262	None:intergenic
GCCAGGTTATGGCGCGTACA+CGG	0.593449	7.2:+15126536	MS.gene52743:CDS
TTGATGGGAAAACGGAAGAG+AGG	0.595310	7.2:+15126670	MS.gene52743:CDS
GATGACGAAGATTCTGGCGA+AGG	0.603346	7.2:+15126581	MS.gene52743:CDS
TGAGTTGAAGGCTTGTTCAG+CGG	0.617005	7.2:-15126307	None:intergenic
AGCCAGTGACGATTGCAACT+CGG	0.618825	7.2:-15126089	None:intergenic
ATTGCAACTCGGTCTTGAAG+TGG	0.619566	7.2:-15126078	None:intergenic
TAACTCAGGATCGAGGACTC+CGG	0.624056	7.2:-15126355	None:intergenic
GCAACTCGGTCTTGAAGTGG+TGG	0.639931	7.2:-15126075	None:intergenic
GGCGCCAGGACCGACTGCGA+CGG	0.642925	7.2:+15126638	MS.gene52743:CDS
TTGGGGAAGTGAAGTTGCCA+TGG	0.648010	7.2:-15126049	None:intergenic
AGAGAGGGCGAAGATGATTG+CGG	0.656517	7.2:+15126686	MS.gene52743:CDS
AGGGATTACTGCAAATTGCG+TGG	0.664845	7.2:+15126617	MS.gene52743:CDS
ACCTTCTGGATCGGATACGT+CGG	0.665516	7.2:-15126265	None:intergenic
ATTCTGGCGAAGGAGCTGAA+GGG	0.667182	7.2:+15126591	MS.gene52743:CDS
TTGATTAGCGTTGATCTCGG+CGG	0.687503	7.2:-15126214	None:intergenic
TAGTTGGTAACTCAGGATCG+AGG	0.712156	7.2:-15126362	None:intergenic
GCGAACAGATTAAAGCGTGG+TGG	0.712295	7.2:+15126465	MS.gene52743:CDS
GGCGCGAACAGATTAAAGCG+TGG	0.716674	7.2:+15126462	MS.gene52743:CDS
GCCGACGTATCCGATCCAGA+AGG	0.719489	7.2:+15126264	MS.gene52743:CDS
CGGCGGCGACAGAGTCAGCG+AGG	0.750380	7.2:-15126197	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AAAGATATTTCACCTGTTGT+TGG	+	chr7.2:15126741-15126760	MS.gene52743:CDS	30.0%
	AAGATATTTCACCTGTTGTT+GGG	+	chr7.2:15126742-15126761	MS.gene52743:CDS	30.0%
!!!	GATGTTTTTTGATGGGAAAA+CGG	+	chr7.2:15126662-15126681	MS.gene52743:CDS	30.0%
	AGTAATCCGTTTGGTAGAAT+TGG	+	chr7.2:15126711-15126730	MS.gene52743:CDS	35.0%
	ATCATAAACGTAAACGCAAG+AGG	+	chr7.2:15126417-15126436	MS.gene52743:CDS	35.0%
	ATTTCTGACAACTTCGTTAG+CGG	+	chr7.2:15126503-15126522	MS.gene52743:CDS	35.0%
	CAAATTATTCGTGTCAATGG+TGG	+	chr7.2:15126801-15126820	MS.gene52743:CDS	35.0%
	GATACGATCGAATGTTTCAA+CGG	-	chr7.2:15126400-15126419	None:intergenic	35.0%
	GGTCAAATTATTCGTGTCAA+TGG	+	chr7.2:15126798-15126817	MS.gene52743:CDS	35.0%
	GTTTCACCAATTCTACCAAA+CGG	-	chr7.2:15126720-15126739	None:intergenic	35.0%
!	AAGAGAGATTTTACACCTTC+TGG	-	chr7.2:15126282-15126301	None:intergenic	35.0%
!	AGATTTTACACCTTCTGGAT+CGG	-	chr7.2:15126277-15126296	None:intergenic	35.0%
!!	TTTGCTATAGTTGGTAACTC+AGG	-	chr7.2:15126372-15126391	None:intergenic	35.0%
	ATTGCGGAAAGTAATCCGTT+TGG	+	chr7.2:15126702-15126721	MS.gene52743:CDS	40.0%
	GAGTTGACTAGGATGTAAAC+CGG	-	chr7.2:15126333-15126352	None:intergenic	40.0%
!	TTCACCTGTTGTTGGGTTTT+TGG	+	chr7.2:15126749-15126768	MS.gene52743:CDS	40.0%
!!	ACGGTTGTGTTTGCTATAGT+TGG	-	chr7.2:15126381-15126400	None:intergenic	40.0%
!!	GATTCTGGTGAATGGGTTAA+TGG	+	chr7.2:15126777-15126796	MS.gene52743:CDS	40.0%
!!	GGTTTGATTAGCGTTGATCT+CGG	-	chr7.2:15126220-15126239	None:intergenic	40.0%
!!!	CGACGGAGATGTTTTTTGAT+GGG	+	chr7.2:15126655-15126674	MS.gene52743:CDS	40.0%
	AAAAACATCTCCGTCGCAGT+CGG	-	chr7.2:15126651-15126670	None:intergenic	45.0%
	AACAGATTAAAGCGTGGTGG+TGG	+	chr7.2:15126468-15126487	MS.gene52743:CDS	45.0%
	AGGGATTACTGCAAATTGCG+TGG	+	chr7.2:15126617-15126636	MS.gene52743:CDS	45.0%
	ATTGCAACTCGGTCTTGAAG+TGG	-	chr7.2:15126081-15126100	None:intergenic	45.0%
	GATGCCAAAAACCCAACAAC+AGG	-	chr7.2:15126756-15126775	None:intergenic	45.0%
	GCGTTTCTCTGTGCAAAAGA+AGG	+	chr7.2:15126441-15126460	MS.gene52743:CDS	45.0%
	GGCATCTGATTCTGGTGAAT+GGG	+	chr7.2:15126770-15126789	MS.gene52743:CDS	45.0%
	TACATCCTAGTCAACTCAGC+CGG	+	chr7.2:15126336-15126355	MS.gene52743:CDS	45.0%
	TGAACAAGCCTTCAACTCAC+CGG	+	chr7.2:15126311-15126330	MS.gene52743:CDS	45.0%
	TGATGGGAAAACGGAAGAGA+GGG	+	chr7.2:15126671-15126690	MS.gene52743:CDS	45.0%
	TGGCATCTGATTCTGGTGAA+TGG	+	chr7.2:15126769-15126788	MS.gene52743:CDS	45.0%
	TTGATGGGAAAACGGAAGAG+AGG	+	chr7.2:15126670-15126689	MS.gene52743:CDS	45.0%
!	GATGTAAACCGGTGAGTTGA+AGG	-	chr7.2:15126322-15126341	None:intergenic	45.0%
!	GTCTTGAAGTGGTGGTGATT+GGG	-	chr7.2:15126070-15126089	None:intergenic	45.0%
!	TCTTGAAGTGGTGGTGATTG+GGG	-	chr7.2:15126069-15126088	None:intergenic	45.0%
!	TGAGTTGAAGGCTTGTTCAG+CGG	-	chr7.2:15126310-15126329	None:intergenic	45.0%
!!	AGTGTAGTTGATGACGAGTC+TGG	-	chr7.2:15126166-15126185	None:intergenic	45.0%
!!	TAGTTGGTAACTCAGGATCG+AGG	-	chr7.2:15126365-15126384	None:intergenic	45.0%
!!	TTGATTAGCGTTGATCTCGG+CGG	-	chr7.2:15126217-15126236	None:intergenic	45.0%
!!!	GCGACGGAGATGTTTTTTGA+TGG	+	chr7.2:15126654-15126673	MS.gene52743:CDS	45.0%
!!!	GGGTTTTTGGCATCTGATTC+TGG	+	chr7.2:15126762-15126781	MS.gene52743:CDS	45.0%
	ACCTTCTGGATCGGATACGT+CGG	-	chr7.2:15126268-15126287	None:intergenic	50.0%
	AGAGAGGGCGAAGATGATTG+CGG	+	chr7.2:15126686-15126705	MS.gene52743:CDS	50.0%
	AGCCAGTGACGATTGCAACT+CGG	-	chr7.2:15126092-15126111	None:intergenic	50.0%
	ATTCTGGCGAAGGAGCTGAA+GGG	+	chr7.2:15126591-15126610	MS.gene52743:CDS	50.0%
	GATGACGAAGATTCTGGCGA+AGG	+	chr7.2:15126581-15126600	MS.gene52743:CDS	50.0%
	GCACTTCACCTTGCTTCACT+CGG	+	chr7.2:15126138-15126157	MS.gene52743:CDS	50.0%
	GCGAACAGATTAAAGCGTGG+TGG	+	chr7.2:15126465-15126484	MS.gene52743:CDS	50.0%
	GCTGCGTTTAAGCCAGGTTA+TGG	+	chr7.2:15126525-15126544	MS.gene52743:CDS	50.0%
	GGAAGCGATGACGAAGATTC+TGG	+	chr7.2:15126575-15126594	MS.gene52743:CDS	50.0%
	TAACTCAGGATCGAGGACTC+CGG	-	chr7.2:15126358-15126377	None:intergenic	50.0%
!	GGTCTTGAAGTGGTGGTGAT+TGG	-	chr7.2:15126071-15126090	None:intergenic	50.0%
!!	AGATTAAAGCGTGGTGGTGG+AGG	+	chr7.2:15126471-15126490	MS.gene52743:CDS	50.0%
!!	GATTAAAGCGTGGTGGTGGA+GGG	+	chr7.2:15126472-15126491	MS.gene52743:CDS	50.0%
	AGAGTCAGCGAGGTTGGAGT+TGG	-	chr7.2:15126190-15126209	None:intergenic	55.0%
	GACCGAGTTGCAATCGTCAC+TGG	+	chr7.2:15126087-15126106	MS.gene52743:CDS	55.0%
	GATTCTGGCGAAGGAGCTGA+AGG	+	chr7.2:15126590-15126609	MS.gene52743:CDS	55.0%
	GCAACTCGGTCTTGAAGTGG+TGG	-	chr7.2:15126078-15126097	None:intergenic	55.0%
	GGCGCGAACAGATTAAAGCG+TGG	+	chr7.2:15126462-15126481	MS.gene52743:CDS	55.0%
	TTAGCGGCTGCGTTTAAGCC+AGG	+	chr7.2:15126519-15126538	MS.gene52743:CDS	55.0%
	TTCTGGATCGGATACGTCGG+CGG	-	chr7.2:15126265-15126284	None:intergenic	55.0%
	TTCTGGCGAAGGAGCTGAAG+GGG	+	chr7.2:15126592-15126611	MS.gene52743:CDS	55.0%
!	ACTGGCTCTTCTCGCGGAAT+CGG	+	chr7.2:15126105-15126124	MS.gene52743:CDS	55.0%
!!	ATCGTCACTGGCTCTTCTCG+CGG	+	chr7.2:15126099-15126118	MS.gene52743:CDS	55.0%
	ACTGCAAATTGCGTGGCGCC+AGG	+	chr7.2:15126624-15126643	MS.gene52743:CDS	60.0%
	ATCGGATACGTCGGCGGGAA+CGG	-	chr7.2:15126259-15126278	None:intergenic	60.0%
	CGAAGGAGCTGAAGGGGACA+GGG	+	chr7.2:15126598-15126617	MS.gene52743:CDS	60.0%
	GCCAGGTTATGGCGCGTACA+CGG	+	chr7.2:15126536-15126555	MS.gene52743:CDS	60.0%
	GCCGACGTATCCGATCCAGA+AGG	+	chr7.2:15126264-15126283	MS.gene52743:CDS	60.0%
	TCTGGATCGGATACGTCGGC+GGG	-	chr7.2:15126264-15126283	None:intergenic	60.0%
!	CGCGTACACGGCATCGAAAG+CGG	+	chr7.2:15126548-15126567	MS.gene52743:CDS	60.0%
!	GCGTACACGGCATCGAAAGC+GGG	+	chr7.2:15126549-15126568	MS.gene52743:CDS	60.0%
!	GGACTCCGGCTGAGTTGACT+AGG	-	chr7.2:15126344-15126363	None:intergenic	60.0%
	ATCTCCGTCGCAGTCGGTCC+TGG	-	chr7.2:15126645-15126664	None:intergenic	65.0%
	GCCGTGTACGCGCCATAACC+TGG	-	chr7.2:15126540-15126559	None:intergenic	65.0%
	GCGAAGGAGCTGAAGGGGAC+AGG	+	chr7.2:15126597-15126616	MS.gene52743:CDS	65.0%
	GGCGACAGAGTCAGCGAGGT+TGG	-	chr7.2:15126196-15126215	None:intergenic	65.0%
	TACGTCGGCGGGAACGGTGA+CGG	-	chr7.2:15126253-15126272	None:intergenic	65.0%
!	AACGGTGACGGCTCGTGGAG+TGG	-	chr7.2:15126241-15126260	None:intergenic	65.0%
!	CACGGCATCGAAAGCGGGAG+TGG	+	chr7.2:15126554-15126573	MS.gene52743:CDS	65.0%
!	GTCTGGCGCCGAGTGAAGCA+AGG	-	chr7.2:15126149-15126168	None:intergenic	65.0%
	CGGCGGCGACAGAGTCAGCG+AGG	-	chr7.2:15126200-15126219	None:intergenic	75.0%
	GGCGCCAGGACCGACTGCGA+CGG	+	chr7.2:15126638-15126657	MS.gene52743:CDS	75.0%
!	GCGGGAACGGTGACGGCTCG+TGG	-	chr7.2:15126246-15126265	None:intergenic	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.2	gene	15126051	15126833	15126051	ID=MS.gene52743
chr7.2	mRNA	15126051	15126833	15126051	ID=MS.gene52743.t1;Parent=MS.gene52743
chr7.2	exon	15126051	15126833	15126051	ID=MS.gene52743.t1.exon1;Parent=MS.gene52743.t1
chr7.2	CDS	15126051	15126833	15126051	ID=cds.MS.gene52743.t1;Parent=MS.gene52743.t1

Gene Sequence

>MS.gene52743

ATGGCAACTTCACTTCCCCAATCACCACCACTTCAAGACCGAGTTGCAATCGTCACTGGCTCTTCTCGCGGAATCGGAAAAGAGATCGCACTTCACCTTGCTTCACTCGGCGCCAGACTCGTCATCAACTACACTTCCAACTCCAACCTCGCTGACTCTGTCGCCGCCGAGATCAACGCTAATCAAACCACTCCACGAGCCGTCACCGTTCCCGCCGACGTATCCGATCCAGAAGGTGTAAAATCTCTCTTCGACTCCGCTGAACAAGCCTTCAACTCACCGGTTTACATCCTAGTCAACTCAGCCGGAGTCCTCGATCCTGAGTTACCAACTATAGCAAACACAACCGTTGAAACATTCGATCGTATCATAAACGTAAACGCAAGAGGAGCGTTTCTCTGTGCAAAAGAAGGCGCGAACAGATTAAAGCGTGGTGGTGGAGGGAGAATTATATTTCTGACAACTTCGTTAGCGGCTGCGTTTAAGCCAGGTTATGGCGCGTACACGGCATCGAAAGCGGGAGTGGAAGCGATGACGAAGATTCTGGCGAAGGAGCTGAAGGGGACAGGGATTACTGCAAATTGCGTGGCGCCAGGACCGACTGCGACGGAGATGTTTTTTGATGGGAAAACGGAAGAGAGGGCGAAGATGATTGCGGAAAGTAATCCGTTTGGTAGAATTGGTGAAACTAAAGATATTTCACCTGTTGTTGGGTTTTTGGCATCTGATTCTGGTGAATGGGTTAATGGTCAAATTATTCGTGTCAATGGTGGCTTTGTTTAG

Protein sequence

>MS.gene52743.t1

MATSLPQSPPLQDRVAIVTGSSRGIGKEIALHLASLGARLVINYTSNSNLADSVAAEINANQTTPRAVTVPADVSDPEGVKSLFDSAEQAFNSPVYILVNSAGVLDPELPTIANTTVETFDRIINVNARGAFLCAKEGANRLKRGGGGRIIFLTTSLAAAFKPGYGAYTASKAGVEAMTKILAKELKGTGITANCVAPGPTATEMFFDGKTEERAKMIAESNPFGRIGETKDISPVVGFLASDSGEWVNGQIIRVNGGFV