AlfalfaGEDB | MS.gene064756

M. sativa cultivar XinJiangDaYe / MS.gene064756

KEGG: -
GO: GO:0003674 GO:0003824 GO:0016491
PFAM: PF13561.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene064756.t1	XP_013450332.1	92.1	265	21	0	5	269	31	295	5.20E-133	483.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene064756.t1	F1SWA0	47.7	256	131	2	6	258	2	257	4.5e-61	236.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene064756.t1	A0A072UF24	92.1	265	21	0	5	269	31	295	3.7e-133	483.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene63306	MS.gene064756	PPI
MS.gene62187	MS.gene064756	PPI
MS.gene064756	MS.gene03319	PPI
MS.gene064756	MS.gene63482	PPI
MS.gene064756	MS.gene043387	PPI
MS.gene064756	MS.gene04700	PPI
MS.gene021164	MS.gene064756	PPI
MS.gene064756	MS.gene08695	PPI
MS.gene064756	MS.gene008161	PPI
MS.gene63483	MS.gene064756	PPI
MS.gene045581	MS.gene064756	PPI
MS.gene064756	MS.gene42114	PPI
MS.gene050431	MS.gene064756	PPI
MS.gene064756	MS.gene039828	PPI
MS.gene003925	MS.gene064756	PPI
MS.gene62193	MS.gene064756	PPI
MS.gene41122	MS.gene064756	PPI
MS.gene064756	MS.gene30551	PPI
MS.gene064756	MS.gene055846	PPI
MS.gene073684	MS.gene064756	PPI
MS.gene63308	MS.gene064756	PPI
MS.gene033685	MS.gene064756	PPI
MS.gene064756	MS.gene63300	PPI
MS.gene064756	MS.gene85724	PPI
MS.gene064756	MS.gene87780	PPI
MS.gene064756	MS.gene008153	PPI
MS.gene012794	MS.gene064756	PPI
MS.gene064756	MS.gene80360	PPI
MS.gene064756	MS.gene008160	PPI
MS.gene064756	MS.gene08756	PPI
MS.gene064756	MS.gene024269	PPI
MS.gene28848	MS.gene064756	PPI
MS.gene87026	MS.gene064756	PPI
MS.gene63307	MS.gene064756	PPI
MS.gene064756	MS.gene007678	PPI
MS.gene006617	MS.gene064756	PPI
MS.gene064756	MS.gene028938	PPI
MS.gene064756	MS.gene003992	PPI
MS.gene73088	MS.gene064756	PPI
MS.gene064756	MS.gene62195	PPI
MS.gene056339	MS.gene064756	PPI
MS.gene07096	MS.gene064756	PPI
MS.gene54952	MS.gene064756	PPI
MS.gene031693	MS.gene064756	PPI
MS.gene050432	MS.gene064756	PPI
MS.gene08694	MS.gene064756	PPI
MS.gene064756	MS.gene018066	PPI
MS.gene064756	MS.gene73824	PPI
MS.gene003926	MS.gene064756	PPI
MS.gene064756	MS.gene003729	PPI
MS.gene064756	MS.gene019786	PPI
MS.gene064756	MS.gene050131	PPI
MS.gene064756	MS.gene43481	PPI
MS.gene62194	MS.gene064756	PPI
MS.gene064756	MS.gene43514	PPI
MS.gene064756	MS.gene52743	PPI
MS.gene044694	MS.gene064756	PPI
MS.gene63484	MS.gene064756	PPI
MS.gene064756	MS.gene54598	PPI
MS.gene064756	MS.gene072664	PPI
MS.gene008162	MS.gene064756	PPI
MS.gene064756	MS.gene07097	PPI
MS.gene064756	MS.gene52742	PPI
MS.gene064756	MS.gene31507	PPI
MS.gene064756	MS.gene28847	PPI
MS.gene015282	MS.gene064756	PPI
MS.gene064756	MS.gene95020	PPI
MS.gene064756	MS.gene065380	PPI
MS.gene09624	MS.gene064756	PPI
MS.gene42856	MS.gene064756	PPI
MS.gene63403	MS.gene064756	PPI
MS.gene064756	MS.gene50524	PPI
MS.gene064756	MS.gene50522	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene064756.t1	MTR_7g107670	92.075	265	21	0	5	269	31	295	0.0	500
MS.gene064756.t1	MTR_3g113870	93.564	202	13	0	19	220	33	234	1.72e-137	387
MS.gene064756.t1	MTR_7g083870	55.639	266	117	1	5	269	33	298	1.45e-106	311
MS.gene064756.t1	MTR_4g052350	49.807	259	121	3	5	262	12	262	1.95e-81	246
MS.gene064756.t1	MTR_4g052340	48.106	264	121	4	5	260	12	267	1.73e-79	241
MS.gene064756.t1	MTR_7g071680	43.382	272	140	4	5	262	27	298	3.56e-79	241
MS.gene064756.t1	MTR_4g052320	48.062	258	126	3	6	262	7	257	1.38e-77	236
MS.gene064756.t1	MTR_4g052300	47.674	258	126	3	6	262	7	256	2.53e-74	228
MS.gene064756.t1	MTR_7g105460	47.287	258	122	3	6	258	12	260	8.00e-70	216
MS.gene064756.t1	MTR_4g078630	43.243	259	140	5	5	259	17	272	1.29e-67	211
MS.gene064756.t1	MTR_7g105580	43.580	257	121	3	4	259	5	238	6.27e-66	206
MS.gene064756.t1	MTR_7g110130	42.529	261	133	5	5	258	4	254	8.23e-66	206
MS.gene064756.t1	MTR_3g020670	44.961	258	139	2	4	258	13	270	2.86e-65	205
MS.gene064756.t1	MTR_7g110140	42.481	266	145	6	6	269	5	264	5.30e-62	196
MS.gene064756.t1	MTR_2g090165	41.016	256	143	4	5	258	4	253	5.84e-62	196
MS.gene064756.t1	MTR_7g105590	52.660	188	88	1	5	191	6	193	1.58e-61	192
MS.gene064756.t1	MTR_6g023590	40.392	255	144	5	6	257	12	261	1.10e-60	193
MS.gene064756.t1	MTR_6g035395	41.065	263	136	5	3	264	1	245	4.54e-60	191
MS.gene064756.t1	MTR_6g023990	40.000	255	145	5	6	257	13	262	1.16e-57	185
MS.gene064756.t1	MTR_7g110160	40.234	256	127	4	5	258	4	235	1.25e-56	182
MS.gene064756.t1	MTR_6g023910	38.931	262	152	4	4	262	10	266	1.47e-56	182
MS.gene064756.t1	MTR_6g023950	40.784	255	143	5	6	257	12	261	8.08e-56	180
MS.gene064756.t1	MTR_6g023610	37.891	256	149	5	6	257	12	261	2.07e-54	177
MS.gene064756.t1	MTR_6g023600	39.216	255	147	5	6	257	12	261	1.90e-53	174
MS.gene064756.t1	MTR_6g023600	38.431	255	143	6	6	257	12	255	3.60e-49	163
MS.gene064756.t1	MTR_7g105570	38.007	271	95	5	2	258	6	217	1.94e-48	160
MS.gene064756.t1	MTR_4g084800	51.493	134	64	1	4	136	10	143	5.04e-40	136
MS.gene064756.t1	MTR_3g085740	28.682	258	167	6	5	257	73	318	1.29e-26	105
MS.gene064756.t1	MTR_4g132850	26.894	264	174	7	1	259	1	250	6.40e-24	97.4
MS.gene064756.t1	MTR_3g068155	28.897	263	169	8	3	260	17	266	5.74e-22	92.4
MS.gene064756.t1	MTR_1196s0010	28.794	257	156	7	9	257	276	513	1.09e-21	94.4
MS.gene064756.t1	MTR_3g068070	28.571	266	162	8	3	258	13	260	2.02e-21	90.9
MS.gene064756.t1	MTR_7g094510	28.409	264	167	9	4	257	3	254	8.86e-21	89.0
MS.gene064756.t1	MTR_3g068085	27.132	258	170	7	7	258	15	260	4.01e-20	87.4
MS.gene064756.t1	MTR_3g068065	26.459	257	175	6	7	259	17	263	5.02e-20	87.4
MS.gene064756.t1	MTR_4g064887	26.718	262	172	7	3	258	13	260	2.12e-18	82.8
MS.gene064756.t1	MTR_7g094520	26.336	262	175	6	4	257	8	259	4.23e-18	81.6
MS.gene064756.t1	MTR_7g063330	28.897	263	170	7	3	259	13	264	8.13e-18	81.3
MS.gene064756.t1	MTR_3g068175	25.954	262	174	7	3	258	15	262	1.81e-17	80.1
MS.gene064756.t1	MTR_7g113650	25.000	268	170	8	4	256	37	288	2.20e-17	80.5
MS.gene064756.t1	MTR_4g131740	27.239	268	165	10	4	256	37	289	5.56e-17	79.3
MS.gene064756.t1	MTR_7g063270	28.358	268	165	7	3	259	14	265	6.51e-17	78.6
MS.gene064756.t1	MTR_1g082760	25.188	266	172	7	4	256	37	288	1.17e-16	78.2
MS.gene064756.t1	MTR_8g442750	30.451	266	156	11	7	257	14	265	1.78e-16	77.8
MS.gene064756.t1	MTR_7g063260	28.517	263	172	7	3	259	14	266	2.06e-16	77.4
MS.gene064756.t1	MTR_3g068175	26.866	268	168	9	3	258	15	266	1.17e-15	75.1
MS.gene064756.t1	MTR_4g064883	25.000	260	179	8	3	258	12	259	2.51e-15	74.3
MS.gene064756.t1	MTR_7g063390	28.626	262	171	8	3	258	14	265	2.67e-15	73.9
MS.gene064756.t1	MTR_4g097510	26.531	196	141	2	8	202	3	196	3.88e-15	73.9
MS.gene064756.t1	MTR_7g063320	28.782	271	157	9	3	257	13	263	6.83e-15	72.8
MS.gene064756.t1	MTR_7g080410	29.381	194	127	5	7	192	38	229	4.69e-14	71.2
MS.gene064756.t1	MTR_7g080410	29.381	194	127	5	7	192	38	229	6.95e-14	70.5
MS.gene064756.t1	MTR_1g051165	25.556	270	175	7	5	258	12	271	1.18e-13	69.7
MS.gene064756.t1	MTR_3g087850	28.415	183	122	4	11	190	30	206	4.39e-13	68.2
MS.gene064756.t1	MTR_1g068955	26.866	268	157	7	7	255	14	261	7.60e-13	67.4
MS.gene064756.t1	MTR_4g058810	26.515	264	171	8	7	258	11	263	1.26e-12	66.6
MS.gene064756.t1	MTR_4g064887	27.273	198	132	5	3	194	13	204	3.91e-12	64.7
MS.gene064756.t1	MTR_7g105560	56.604	53	23	0	4	56	10	62	4.00e-12	63.2
MS.gene064756.t1	MTR_4g058840	26.437	261	175	7	7	258	11	263	5.02e-12	65.1
MS.gene064756.t1	MTR_4g058820	26.437	261	175	7	7	258	11	263	5.02e-12	65.1
MS.gene064756.t1	MTR_4g064883	25.888	197	138	5	3	195	12	204	2.65e-11	62.0
MS.gene064756.t1	MTR_8g044240	25.773	194	134	4	13	196	75	268	2.99e-11	63.2
MS.gene064756.t1	MTR_3g087860	26.036	169	117	3	24	190	29	191	6.86e-11	61.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene064756.t1	AT4G03140	70.566	265	78	0	5	269	76	340	1.16e-138	394
MS.gene064756.t1	AT3G26770	54.104	268	120	2	4	269	38	304	6.30e-105	307
MS.gene064756.t1	AT3G26760	52.060	267	125	2	4	267	33	299	2.33e-101	298
MS.gene064756.t1	AT2G47120	43.346	263	139	6	1	261	1	255	1.21e-71	221
MS.gene064756.t1	AT2G47120	43.798	258	135	6	6	261	5	254	1.51e-71	220
MS.gene064756.t1	AT3G51680	43.750	272	141	4	3	262	28	299	1.64e-71	222
MS.gene064756.t1	AT3G29250	45.349	258	133	4	6	261	5	256	2.99e-71	220
MS.gene064756.t1	AT3G29250	43.636	275	135	5	1	261	24	292	4.14e-71	221
MS.gene064756.t1	AT2G47130	44.788	259	132	6	6	261	5	255	7.81e-71	219
MS.gene064756.t1	AT3G29250	43.682	277	134	6	1	261	24	294	1.80e-70	219
MS.gene064756.t1	AT3G29260	42.308	260	139	5	5	261	4	255	4.24e-68	212
MS.gene064756.t1	AT2G47140	40.154	259	147	5	5	261	4	256	7.18e-64	201
MS.gene064756.t1	AT1G52340	45.290	276	123	7	5	261	16	282	1.17e-61	196
MS.gene064756.t1	AT3G42960	41.923	260	143	6	5	259	6	262	5.36e-58	186
MS.gene064756.t1	AT2G47150	30.859	256	118	5	7	261	1	198	4.48e-30	112
MS.gene064756.t1	AT1G07450	28.846	260	168	7	3	258	6	252	4.04e-23	95.5
MS.gene064756.t1	AT5G06060	26.923	260	174	6	3	258	7	254	9.69e-22	91.7
MS.gene064756.t1	AT2G29310	28.846	260	169	7	3	258	5	252	1.25e-21	91.7
MS.gene064756.t1	AT1G24360	28.295	258	168	7	5	257	72	317	1.75e-21	92.0
MS.gene064756.t1	AT4G05530	28.846	260	166	7	5	259	7	252	3.63e-21	90.1
MS.gene064756.t1	AT2G29340	27.652	264	167	7	3	258	5	252	6.75e-21	89.4
MS.gene064756.t1	AT2G29340	27.652	264	167	7	3	258	5	252	1.10e-20	89.7
MS.gene064756.t1	AT1G63380	29.167	264	167	9	6	258	14	268	1.73e-20	88.6
MS.gene064756.t1	AT1G63380	29.167	264	167	9	6	258	16	270	2.15e-20	88.6
MS.gene064756.t1	AT1G63380	29.167	264	167	9	6	258	21	275	2.27e-20	88.6
MS.gene064756.t1	AT1G63380	29.167	264	167	9	6	258	18	272	2.50e-20	88.2
MS.gene064756.t1	AT5G18210	29.167	264	162	9	7	257	8	259	2.63e-20	87.8
MS.gene064756.t1	AT2G30670	27.692	260	172	7	3	258	5	252	1.98e-19	85.5
MS.gene064756.t1	AT2G29330	27.308	260	173	7	3	258	5	252	2.55e-19	85.1
MS.gene064756.t1	AT2G29360	28.413	271	170	9	3	263	14	270	3.36e-19	85.1
MS.gene064756.t1	AT2G29370	26.923	260	174	6	3	258	14	261	3.73e-19	84.7
MS.gene064756.t1	AT1G62610	28.409	264	169	9	6	258	12	266	5.20e-19	84.7
MS.gene064756.t1	AT1G54870	27.341	267	165	8	4	256	79	330	5.47e-19	85.5
MS.gene064756.t1	AT1G62610	28.409	264	169	9	6	258	14	268	5.75e-19	84.7
MS.gene064756.t1	AT1G62610	28.409	264	169	9	6	258	13	267	5.96e-19	84.3
MS.gene064756.t1	AT1G54870	27.341	267	165	8	4	256	81	332	6.80e-19	85.1
MS.gene064756.t1	AT1G62610	28.409	264	169	9	6	258	18	272	7.37e-19	84.3
MS.gene064756.t1	AT2G29300	25.843	267	173	8	3	260	5	255	3.10e-18	82.4
MS.gene064756.t1	AT2G29300	25.843	267	173	8	3	260	5	255	3.27e-18	82.4
MS.gene064756.t1	AT2G17845	28.571	266	164	8	6	256	46	300	3.87e-18	82.8
MS.gene064756.t1	AT3G46170	29.389	262	168	7	6	258	25	278	4.44e-18	82.4
MS.gene064756.t1	AT3G55290	30.075	266	161	9	6	258	16	269	4.53e-18	82.0
MS.gene064756.t1	AT2G29350	28.044	271	170	8	3	263	13	268	4.72e-18	82.0
MS.gene064756.t1	AT3G55290	30.075	266	161	9	6	258	17	270	4.78e-18	82.0
MS.gene064756.t1	AT2G29150	27.692	260	167	8	3	256	14	258	9.98e-18	80.9
MS.gene064756.t1	AT2G29320	27.444	266	166	8	3	258	11	259	1.20e-17	80.9
MS.gene064756.t1	AT3G03980	28.195	266	166	7	7	257	14	269	1.58e-17	80.5
MS.gene064756.t1	AT3G55310	30.451	266	160	9	6	258	16	269	4.85e-17	79.3
MS.gene064756.t1	AT2G29260	25.830	271	183	8	3	269	66	322	1.02e-16	79.0
MS.gene064756.t1	AT3G04000	26.471	272	162	6	7	256	14	269	1.05e-16	78.2
MS.gene064756.t1	AT2G29290	25.573	262	175	6	3	258	5	252	1.78e-16	77.4
MS.gene064756.t1	AT5G18210	29.167	264	149	10	7	257	8	246	2.95e-16	77.0
MS.gene064756.t1	AT3G05260	25.283	265	174	6	4	257	34	285	4.31e-16	76.6
MS.gene064756.t1	AT1G07440	26.154	260	175	7	3	258	10	256	7.57e-16	75.9
MS.gene064756.t1	AT1G49670	30.256	195	121	4	12	192	9	202	8.81e-16	77.4
MS.gene064756.t1	AT1G49670	30.256	195	121	4	12	192	9	202	9.63e-16	77.0
MS.gene064756.t1	AT4G13180	26.236	263	174	7	4	257	10	261	2.59e-15	74.3
MS.gene064756.t1	AT3G12800	28.623	276	167	9	7	266	10	271	3.58e-15	74.3
MS.gene064756.t1	AT2G29310	26.957	230	154	6	33	258	31	250	7.20e-15	72.8
MS.gene064756.t1	AT2G29340	27.551	196	134	5	3	194	5	196	1.54e-14	70.9
MS.gene064756.t1	AT2G29340	27.551	196	134	5	3	194	5	196	1.54e-14	70.9
MS.gene064756.t1	AT5G04900	26.943	193	132	3	13	196	83	275	2.48e-13	69.3
MS.gene064756.t1	AT2G29310	27.551	196	134	4	3	194	5	196	3.05e-13	67.4
MS.gene064756.t1	AT2G29350	26.638	229	150	6	40	263	15	230	6.29e-13	67.0
MS.gene064756.t1	AT3G47350	28.358	201	131	7	10	203	47	241	1.12e-12	67.4
MS.gene064756.t1	AT3G47350	28.358	201	131	7	10	203	47	241	1.45e-12	67.0
MS.gene064756.t1	AT1G10310	29.762	168	115	2	10	175	18	184	3.98e-12	64.7
MS.gene064756.t1	AT5G65205	27.957	186	125	4	8	190	7	186	5.34e-12	65.1
MS.gene064756.t1	AT2G29290	23.423	222	153	5	40	258	28	235	5.48e-11	61.6
MS.gene064756.t1	AT2G29320	27.179	195	133	5	3	192	11	201	7.89e-11	60.8

Find 61 sgRNAs with CRISPR-Local

Find 64 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GTTCACAATTCGCTCCTTTC+AGG	0.161977	3.4:-97855910	None:intergenic
GACAAGGTAGCACTCATAAT+TGG	0.175849	3.4:+97855275	MS.gene064756:CDS
GTATCCAAGTTTGCGGTTAT+AGG	0.264110	3.4:+97855725	MS.gene064756:CDS
GCAAGAAAATTCATCAATAA+TGG	0.327600	3.4:+97855326	MS.gene064756:CDS
TCTTTCTAATGCACTTAATC+TGG	0.330602	3.4:-97856052	None:intergenic
ACAAGGTAGCACTCATAATT+GGG	0.370528	3.4:+97855276	MS.gene064756:CDS
GTAATGATCCCGCGTGGAAC+TGG	0.374917	3.4:+97855638	MS.gene064756:CDS
AATAAATGTTTGTGGCGTTA+TGG	0.400168	3.4:+97855592	MS.gene064756:CDS
CAAGTTATGAAGGTAGCATT+AGG	0.406451	3.4:-97855413	None:intergenic
AGATCGAGGTCAATAACGTT+TGG	0.408258	3.4:-97855545	None:intergenic
TGCAGCATTAGCAATATCAT+TGG	0.411118	3.4:-97855933	None:intergenic
TTTCCTGGCCAAGTTGTTGT+TGG	0.418117	3.4:-97855373	None:intergenic
ATTGACTCGGATTTCATGCC+TGG	0.424967	3.4:-97855777	None:intergenic
GTAGAAATTGTTCATAATGT+TGG	0.425856	3.4:+97855884	MS.gene064756:CDS
CTATGCACTGCCAGTGTCAC+TGG	0.430322	3.4:+97855668	MS.gene064756:CDS
AATGCCTATAACCGCAAACT+TGG	0.438642	3.4:-97855729	None:intergenic
CCATGGCTTCAGAGCTATCC+AGG	0.442092	3.4:+97855759	MS.gene064756:CDS
GCCAGTGTCACTGGTGTGAT+TGG	0.442709	3.4:+97855677	MS.gene064756:CDS
TTCATTTACGTTTGATGTGG+TGG	0.450297	3.4:+97855237	None:intergenic
GTATATCAGCGGGCATACTT+TGG	0.458992	3.4:+97855982	MS.gene064756:CDS
GATATCATGTATAACAATGC+AGG	0.475659	3.4:+97855503	MS.gene064756:CDS
TAATGCACTTAATCTGGTGC+AGG	0.475984	3.4:-97856046	None:intergenic
ATACTCTGTATCCAAGTTTG+CGG	0.478404	3.4:+97855718	MS.gene064756:CDS
GTCATGAAAATAAATGTTTG+TGG	0.480828	3.4:+97855584	MS.gene064756:CDS
TATAGGCATTGTTAAATCCA+TGG	0.486525	3.4:+97855742	MS.gene064756:CDS
AAGTTGTTTGTATTCAGAAA+CGG	0.501899	3.4:-97855480	None:intergenic
TCCATTTCGCACATGACAAA+AGG	0.506027	3.4:-97855827	None:intergenic
GCAGCATTAGCAATATCATT+GGG	0.508284	3.4:-97855932	None:intergenic
AATGTTTGTGGCGTTATGGC+TGG	0.510119	3.4:+97855596	MS.gene064756:CDS
TGTGACGTTGCAAGTTATGA+AGG	0.510852	3.4:-97855423	None:intergenic
CAGGAAACTGCAAAAGAACT+AGG	0.510876	3.4:+97855389	MS.gene064756:CDS
ACCTTGCATCTAATGATGCT+AGG	0.517372	3.4:+97855960	MS.gene064756:CDS
TACGTTTGATGTGGTGGTCA+AGG	0.518029	3.4:+97855243	None:intergenic
GACACTGGCAGTGCATAGAA+TGG	0.519009	3.4:-97855663	None:intergenic
CCTGGATAGCTCTGAAGCCA+TGG	0.523976	3.4:-97855759	None:intergenic
CATACTTTGGCTGTGGATGG+AGG	0.528711	3.4:+97855995	MS.gene064756:CDS
AGTGTCACTGGTGTGATTGG+AGG	0.535327	3.4:+97855680	MS.gene064756:CDS
CACTGGTGTGATTGGAGGGA+TGG	0.539300	3.4:+97855685	MS.gene064756:CDS
CAGCGGGCATACTTTGGCTG+TGG	0.539481	3.4:+97855988	MS.gene064756:CDS
TCCAATCACACCAGTGACAC+TGG	0.545725	3.4:-97855678	None:intergenic
GTGTCACTGGTGTGATTGGA+GGG	0.547063	3.4:+97855681	MS.gene064756:CDS
GCTGATATCCAACAACAACT+TGG	0.550507	3.4:+97855365	MS.gene064756:CDS
GGGCATACTTTGGCTGTGGA+TGG	0.556081	3.4:+97855992	MS.gene064756:CDS
TCGAGGTCAATAACGTTTGG+AGG	0.558071	3.4:-97855542	None:intergenic
TATCCAACAACAACTTGGCC+AGG	0.561816	3.4:+97855370	MS.gene064756:CDS
ACCTAGCATCATTAGATGCA+AGG	0.564196	3.4:-97855961	None:intergenic
CATAATGTTGGAGTCCTGAA+AGG	0.573360	3.4:+97855896	MS.gene064756:CDS
AAAGGAGTAGGAATAGCAAA+AGG	0.575525	3.4:-97855809	None:intergenic
ATATCATGTATAACAATGCA+GGG	0.592185	3.4:+97855504	MS.gene064756:CDS
TCGCACATGACAAAAGGAGT+AGG	0.606020	3.4:-97855821	None:intergenic
CAAGGTAGCACTCATAATTG+GGG	0.609725	3.4:+97855277	MS.gene064756:CDS
GTCAAGGAAGCTACAAGACA+AGG	0.659741	3.4:+97855259	MS.gene064756:CDS
ATGATGCTAGGTATATCAGC+GGG	0.660569	3.4:+97855972	MS.gene064756:CDS
AAGGCGATATACAATTGACT+CGG	0.673213	3.4:-97855790	None:intergenic
AGTCGCTGCGAATCAAGATG+TGG	0.674277	3.4:-97855860	None:intergenic
GAATGGATCCAGTTCCACGC+GGG	0.678631	3.4:-97855646	None:intergenic
TGTCAAACGATTCTAGATCG+AGG	0.680208	3.4:-97855559	None:intergenic
AATGATGCTAGGTATATCAG+CGG	0.703024	3.4:+97855971	MS.gene064756:CDS
GCGCGTGTAATGATCCCGCG+TGG	0.726381	3.4:+97855632	MS.gene064756:CDS
AAGGTAGCACTCATAATTGG+GGG	0.728756	3.4:+97855278	MS.gene064756:CDS
AGAATGGATCCAGTTCCACG+CGG	0.782409	3.4:-97855647	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTCACATCTTTTAAGAATT+TGG	+	chr3.4:97856018-97856037	MS.gene064756:CDS	20.0%
!	AAGTTGTTTGTATTCAGAAA+CGG	-	chr3.4:97855483-97855502	None:intergenic	25.0%
!	ATATCATGTATAACAATGCA+GGG	+	chr3.4:97855504-97855523	MS.gene064756:CDS	25.0%
!	GCAAGAAAATTCATCAATAA+TGG	+	chr3.4:97855326-97855345	MS.gene064756:CDS	25.0%
!	GTAGAAATTGTTCATAATGT+TGG	+	chr3.4:97855884-97855903	MS.gene064756:CDS	25.0%
!	GTCATGAAAATAAATGTTTG+TGG	+	chr3.4:97855584-97855603	MS.gene064756:CDS	25.0%
	AATAAATGTTTGTGGCGTTA+TGG	+	chr3.4:97855592-97855611	MS.gene064756:CDS	30.0%
	GATATCATGTATAACAATGC+AGG	+	chr3.4:97855503-97855522	MS.gene064756:CDS	30.0%
!	TATAGGCATTGTTAAATCCA+TGG	+	chr3.4:97855742-97855761	MS.gene064756:CDS	30.0%
	AAAGGAGTAGGAATAGCAAA+AGG	-	chr3.4:97855812-97855831	None:intergenic	35.0%
	AAGGCGATATACAATTGACT+CGG	-	chr3.4:97855793-97855812	None:intergenic	35.0%
	AATGATGCTAGGTATATCAG+CGG	+	chr3.4:97855971-97855990	MS.gene064756:CDS	35.0%
	ACAAGGTAGCACTCATAATT+GGG	+	chr3.4:97855276-97855295	MS.gene064756:CDS	35.0%
	ATACTCTGTATCCAAGTTTG+CGG	+	chr3.4:97855718-97855737	MS.gene064756:CDS	35.0%
	CAAGTTATGAAGGTAGCATT+AGG	-	chr3.4:97855416-97855435	None:intergenic	35.0%
	GCAGCATTAGCAATATCATT+GGG	-	chr3.4:97855935-97855954	None:intergenic	35.0%
	TGCAGCATTAGCAATATCAT+TGG	-	chr3.4:97855936-97855955	None:intergenic	35.0%
	AAGGTAGCACTCATAATTGG+GGG	+	chr3.4:97855278-97855297	MS.gene064756:CDS	40.0%
	AATGCCTATAACCGCAAACT+TGG	-	chr3.4:97855732-97855751	None:intergenic	40.0%
	ACCTAGCATCATTAGATGCA+AGG	-	chr3.4:97855964-97855983	None:intergenic	40.0%
	ACCTTGCATCTAATGATGCT+AGG	+	chr3.4:97855960-97855979	MS.gene064756:CDS	40.0%
	AGATCGAGGTCAATAACGTT+TGG	-	chr3.4:97855548-97855567	None:intergenic	40.0%
	ATGATGCTAGGTATATCAGC+GGG	+	chr3.4:97855972-97855991	MS.gene064756:CDS	40.0%
	CAAGGTAGCACTCATAATTG+GGG	+	chr3.4:97855277-97855296	MS.gene064756:CDS	40.0%
	CAGGAAACTGCAAAAGAACT+AGG	+	chr3.4:97855389-97855408	MS.gene064756:CDS	40.0%
	CATAATGTTGGAGTCCTGAA+AGG	+	chr3.4:97855896-97855915	MS.gene064756:CDS	40.0%
	GACAAGGTAGCACTCATAAT+TGG	+	chr3.4:97855275-97855294	MS.gene064756:CDS	40.0%
	GCTGATATCCAACAACAACT+TGG	+	chr3.4:97855365-97855384	MS.gene064756:CDS	40.0%
	GTATCCAAGTTTGCGGTTAT+AGG	+	chr3.4:97855725-97855744	MS.gene064756:CDS	40.0%
	TCCATTTCGCACATGACAAA+AGG	-	chr3.4:97855830-97855849	None:intergenic	40.0%
	TGTCAAACGATTCTAGATCG+AGG	-	chr3.4:97855562-97855581	None:intergenic	40.0%
	TGTGACGTTGCAAGTTATGA+AGG	-	chr3.4:97855426-97855445	None:intergenic	40.0%
!	TCCTTTTGTCATGTGCGAAA+TGG	+	chr3.4:97855826-97855845	MS.gene064756:CDS	40.0%
!!	CTAGTTCTTTTGCAGTTTCC+TGG	-	chr3.4:97855391-97855410	None:intergenic	40.0%
	AATGTTTGTGGCGTTATGGC+TGG	+	chr3.4:97855596-97855615	MS.gene064756:CDS	45.0%
	GTATATCAGCGGGCATACTT+TGG	+	chr3.4:97855982-97856001	MS.gene064756:CDS	45.0%
	GTCAAGGAAGCTACAAGACA+AGG	+	chr3.4:97855259-97855278	MS.gene064756:CDS	45.0%
	GTTCACAATTCGCTCCTTTC+AGG	-	chr3.4:97855913-97855932	None:intergenic	45.0%
	TATCCAACAACAACTTGGCC+AGG	+	chr3.4:97855370-97855389	MS.gene064756:CDS	45.0%
	TCGAGGTCAATAACGTTTGG+AGG	-	chr3.4:97855545-97855564	None:intergenic	45.0%
	TCGCACATGACAAAAGGAGT+AGG	-	chr3.4:97855824-97855843	None:intergenic	45.0%
	TTTCCTGGCCAAGTTGTTGT+TGG	-	chr3.4:97855376-97855395	None:intergenic	45.0%
!	AACGTTTGGAGGAGTTTTGC+AGG	-	chr3.4:97855534-97855553	None:intergenic	45.0%
!	ACGTTTGGAGGAGTTTTGCA+GGG	-	chr3.4:97855533-97855552	None:intergenic	45.0%
!!	ATTGACTCGGATTTCATGCC+TGG	-	chr3.4:97855780-97855799	None:intergenic	45.0%
	AGAATGGATCCAGTTCCACG+CGG	-	chr3.4:97855650-97855669	None:intergenic	50.0%
	AGTCGCTGCGAATCAAGATG+TGG	-	chr3.4:97855863-97855882	None:intergenic	50.0%
	ATTGGGGGAGCTAGTGGAAT+AGG	+	chr3.4:97855293-97855312	MS.gene064756:CDS	50.0%
	CATACTTTGGCTGTGGATGG+AGG	+	chr3.4:97855995-97856014	MS.gene064756:CDS	50.0%
	CTCATAATTGGGGGAGCTAG+TGG	+	chr3.4:97855287-97855306	MS.gene064756:CDS	50.0%
	GACACTGGCAGTGCATAGAA+TGG	-	chr3.4:97855666-97855685	None:intergenic	50.0%
	TCCAATCACACCAGTGACAC+TGG	-	chr3.4:97855681-97855700	None:intergenic	50.0%
!	AGTGTCACTGGTGTGATTGG+AGG	+	chr3.4:97855680-97855699	MS.gene064756:CDS	50.0%
!	GTGTCACTGGTGTGATTGGA+GGG	+	chr3.4:97855681-97855700	MS.gene064756:CDS	50.0%
	CACTGGTGTGATTGGAGGGA+TGG	+	chr3.4:97855685-97855704	MS.gene064756:CDS	55.0%
	CCATGGCTTCAGAGCTATCC+AGG	+	chr3.4:97855759-97855778	MS.gene064756:CDS	55.0%
	CTATGCACTGCCAGTGTCAC+TGG	+	chr3.4:97855668-97855687	MS.gene064756:CDS	55.0%
	GAATGGATCCAGTTCCACGC+GGG	-	chr3.4:97855649-97855668	None:intergenic	55.0%
	GGGCATACTTTGGCTGTGGA+TGG	+	chr3.4:97855992-97856011	MS.gene064756:CDS	55.0%
	GTAATGATCCCGCGTGGAAC+TGG	+	chr3.4:97855638-97855657	MS.gene064756:CDS	55.0%
!	GCCAGTGTCACTGGTGTGAT+TGG	+	chr3.4:97855677-97855696	MS.gene064756:CDS	55.0%
!!	CCTGGATAGCTCTGAAGCCA+TGG	-	chr3.4:97855762-97855781	None:intergenic	55.0%
	CAGCGGGCATACTTTGGCTG+TGG	+	chr3.4:97855988-97856007	MS.gene064756:CDS	60.0%
	GCGCGTGTAATGATCCCGCG+TGG	+	chr3.4:97855632-97855651	MS.gene064756:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.4	gene	97855251	97856060	97855251	ID=MS.gene064756
chr3.4	mRNA	97855251	97856060	97855251	ID=MS.gene064756.t1;Parent=MS.gene064756
chr3.4	exon	97855251	97856060	97855251	ID=MS.gene064756.t1.exon1;Parent=MS.gene064756.t1
chr3.4	CDS	97855251	97856060	97855251	ID=cds.MS.gene064756.t1;Parent=MS.gene064756.t1

Gene Sequence

>MS.gene064756

ATGTGGTGGTCAAGGAAGCTACAAGACAAGGTAGCACTCATAATTGGGGGAGCTAGTGGAATAGGAAAAGCTGCAGCAAGAAAATTCATCAATAATGGAGCAAAAGTAATTATTGCTGATATCCAACAACAACTTGGCCAGGAAACTGCAAAAGAACTAGGACCTAATGCTACCTTCATAACTTGCAACGTCACAAAAGAATCAGACATATCAGATGCAGTTGATTTCGCCGTTTCTGAATACAAACAACTTGATATCATGTATAACAATGCAGGGATACCCTGCAAAACTCCTCCAAACGTTATTGACCTCGATCTAGAATCGTTTGACAAAGTCATGAAAATAAATGTTTGTGGCGTTATGGCTGGCATCAAGCATGCAGCGCGTGTAATGATCCCGCGTGGAACTGGATCCATTCTATGCACTGCCAGTGTCACTGGTGTGATTGGAGGGATGGCGCAGCATACATACTCTGTATCCAAGTTTGCGGTTATAGGCATTGTTAAATCCATGGCTTCAGAGCTATCCAGGCATGAAATCCGAGTCAATTGTATATCGCCTTTTGCTATTCCTACTCCTTTTGTCATGTGCGAAATGGATCAGATATATCCACATCTTGATTCGCAGCGACTTGTAGAAATTGTTCATAATGTTGGAGTCCTGAAAGGAGCGAATTGTGAACCCAATGATATTGCTAATGCTGCACTTTACCTTGCATCTAATGATGCTAGGTATATCAGCGGGCATACTTTGGCTGTGGATGGAGGTTTCACATCTTTTAAGAATTTGGAGTTTCCTGCACCAGATTAA

Protein sequence

>MS.gene064756.t1

MWWSRKLQDKVALIIGGASGIGKAAARKFINNGAKVIIADIQQQLGQETAKELGPNATFITCNVTKESDISDAVDFAVSEYKQLDIMYNNAGIPCKTPPNVIDLDLESFDKVMKINVCGVMAGIKHAARVMIPRGTGSILCTASVTGVIGGMAQHTYSVSKFAVIGIVKSMASELSRHEIRVNCISPFAIPTPFVMCEMDQIYPHLDSQRLVEIVHNVGVLKGANCEPNDIANAALYLASNDARYISGHTLAVDGGFTSFKNLEFPAPD