AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene59273


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene59273.t1 MTR_6g059530 93.103 203 12 1 1 201 1 203 1.39e-125 352
MS.gene59273.t1 MTR_6g059410 92.118 203 13 2 1 201 1 202 2.18e-123 347
MS.gene59273.t1 MTR_6g059650 55.155 194 77 5 17 201 19 211 4.22e-65 199
MS.gene59273.t1 MTR_6g065460 53.398 206 81 7 1 193 1 204 2.57e-55 174
MS.gene59273.t1 MTR_6g059760 47.847 209 92 11 1 196 1 205 1.86e-47 155
MS.gene59273.t1 MTR_6g065460 48.756 201 74 6 1 193 1 180 3.28e-45 148
MS.gene59273.t1 MTR_6g059680 47.525 202 87 8 16 201 18 216 7.85e-42 140
MS.gene59273.t1 MTR_6g044780 41.860 215 106 10 1 199 1 212 2.45e-40 137
MS.gene59273.t1 MTR_3g463590 43.617 188 93 7 24 201 27 211 9.99e-40 135
MS.gene59273.t1 MTR_6g045097 40.930 215 108 10 1 199 1 212 3.10e-38 132
MS.gene59273.t1 MTR_6g044810 40.930 215 108 10 1 199 1 212 2.94e-37 129
MS.gene59273.t1 MTR_6g059730 44.186 215 103 9 1 201 1 212 8.93e-37 127
MS.gene59273.t1 MTR_6g045403 40.187 214 109 9 1 199 1 210 2.39e-35 124
MS.gene59273.t1 MTR_6g017225 40.187 214 106 11 6 201 7 216 7.72e-35 126
MS.gene59273.t1 MTR_6g009650 42.079 202 99 8 6 194 7 203 8.33e-35 122
MS.gene59273.t1 MTR_6g066190 39.409 203 105 8 6 195 7 204 1.23e-32 117
MS.gene59273.t1 MTR_6g009563 40.796 201 102 8 6 194 9 204 2.87e-32 115
MS.gene59273.t1 MTR_6g045433 35.931 231 106 11 1 201 1 219 6.67e-31 112
MS.gene59273.t1 MTR_6g066210 38.679 212 115 8 1 199 1 210 3.34e-29 108
MS.gene59273.t1 MTR_6g059810 38.647 207 101 10 4 190 8 208 4.97e-29 107
MS.gene59273.t1 MTR_1g035770 39.320 206 103 10 6 192 7 209 3.23e-26 100
MS.gene59273.t1 MTR_6g059755 49.074 108 49 4 98 199 18 125 4.64e-26 97.8
MS.gene59273.t1 MTR_6g078070 34.146 205 121 9 1 193 1 203 1.16e-21 88.2
MS.gene59273.t1 MTR_6g078100 33.654 208 120 9 1 193 1 205 4.76e-20 84.0
MS.gene59273.t1 MTR_1g057110 36.538 208 97 13 1 194 1 187 4.51e-17 75.9
MS.gene59273.t1 MTR_6g065570 53.333 60 25 1 16 72 14 73 1.56e-15 69.3
MS.gene59273.t1 MTR_0100s0150 26.471 204 120 8 7 190 5 198 6.55e-14 67.8
MS.gene59273.t1 MTR_0084s0030 28.283 198 125 8 7 190 5 199 6.52e-13 65.1
MS.gene59273.t1 MTR_3g106620 31.492 181 107 9 24 194 27 200 1.04e-12 64.7
MS.gene59273.t1 MTR_0211s0080 27.320 194 128 7 7 190 5 195 2.95e-12 63.2
MS.gene59273.t1 MTR_6g078290 30.233 215 119 11 1 190 1 209 4.51e-12 62.8
MS.gene59273.t1 MTR_0302s0030 25.854 205 121 8 7 190 5 199 7.18e-12 62.0
MS.gene59273.t1 MTR_2g073010 28.431 204 124 8 1 193 6 198 4.19e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene59273.t1 AT1G73260 35.326 184 105 7 25 197 29 209 3.18e-22 90.1
MS.gene59273.t1 AT1G17860 30.882 204 121 9 1 193 1 195 1.22e-16 75.1

Find 54 sgRNAs with CRISPR-Local

Find 56 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTAAGTAATGTTGACTTCA+TGG 0.276702 6.4:-44658982 None:intergenic
CAACTGAAAATGATCCCTTT+AGG 0.293355 6.4:+44659522 MS.gene59273:CDS
AACTGAAAATGATCCCTTTA+GGG 0.300397 6.4:+44659523 MS.gene59273:CDS
GAAACAGTTGAAGACATAAA+TGG 0.316517 6.4:+44659053 MS.gene59273:CDS
AAATCTTGTAGAACAGTAAC+TGG 0.355500 6.4:-44659176 None:intergenic
ACTGTTTCAGTAAAAGCTAA+TGG 0.364363 6.4:-44659038 None:intergenic
CCTGGTGCTTGTAGTGATAT+TGG 0.364762 6.4:+44659464 MS.gene59273:CDS
CCTACATCTTGGGTAGGTAT+TGG 0.367664 6.4:+44659347 MS.gene59273:CDS
AATGGAAACCCCGTATTTCC+TGG 0.375075 6.4:+44659071 MS.gene59273:CDS
ACTCGTATGATAAATCTAAC+TGG 0.385923 6.4:-44659221 None:intergenic
AAAATTGAGACACTTGGTTC+AGG 0.392743 6.4:+44659401 MS.gene59273:CDS
GATAATATTGACGCATTTGA+AGG 0.419997 6.4:+44659371 MS.gene59273:CDS
ATGATGATTTCCCTACATCT+TGG 0.421230 6.4:+44659336 MS.gene59273:CDS
CTGGTGCTTGTAGTGATATT+GGG 0.426790 6.4:+44659465 MS.gene59273:CDS
AGTAGGTAAAGGAGAGGAGG+AGG 0.443614 6.4:-44659013 None:intergenic
TGATGATTTCCCTACATCTT+GGG 0.444534 6.4:+44659337 MS.gene59273:CDS
TCAACAAATACAACCCTAAA+GGG 0.446150 6.4:-44659536 None:intergenic
GTATTTGCTACCAGGAAATA+CGG 0.450228 6.4:-44659081 None:intergenic
CCAATACCTACCCAAGATGT+AGG 0.454772 6.4:-44659347 None:intergenic
TATTTGCTACCAGGAAATAC+GGG 0.456020 6.4:-44659080 None:intergenic
AGATGAGAATGGATGGCGTT+TGG 0.471954 6.4:+44659496 MS.gene59273:CDS
ATCATGAGCTAGCACCCATT+TGG 0.477611 6.4:-44659318 None:intergenic
GGAGAGGAGGAGGAGAGAAA+AGG 0.484460 6.4:-44659003 None:intergenic
ATCAACAAATACAACCCTAA+AGG 0.485036 6.4:-44659537 None:intergenic
AGTAAAAGCTAATGGAAAGT+AGG 0.489860 6.4:-44659030 None:intergenic
TGGAGATTTGATTCAAACTA+CGG 0.490153 6.4:-44659579 None:intergenic
GGATTAAAGCTTGGTAAGAC+AGG 0.490806 6.4:+44659140 MS.gene59273:CDS
AGCTAATGGAAAGTAGGTAA+AGG 0.506699 6.4:-44659024 None:intergenic
ATGTCTAAAGGAGGAGGTGG+TGG 0.513667 6.4:+44659119 MS.gene59273:CDS
GGAGGTGGTGGATTAAAGCT+TGG 0.515566 6.4:+44659131 MS.gene59273:CDS
GGGAGGTATAGAGATGAGAA+TGG 0.518670 6.4:+44659485 MS.gene59273:CDS
CCTCCTCCTTTAGACATAAG+TGG 0.521949 6.4:-44659113 None:intergenic
GTGTGCTGAATCTGCCAAAT+GGG 0.523391 6.4:+44659304 MS.gene59273:CDS
CCAAGGTTCAGTGCTCCACC+TGG 0.525036 6.4:+44659446 MS.gene59273:CDS
AGTGTGCTGAATCTGCCAAA+TGG 0.527935 6.4:+44659303 MS.gene59273:CDS
TTGGCAGATTCAGCACACTT+TGG 0.530934 6.4:-44659299 None:intergenic
ATTGCACCACTTATGTCTAA+AGG 0.535923 6.4:+44659107 MS.gene59273:CDS
CCAGGTGGAGCACTGAACCT+TGG 0.547779 6.4:-44659446 None:intergenic
CAATACCTACCCAAGATGTA+GGG 0.570789 6.4:-44659346 None:intergenic
ACAAGCTTGTGTATTGTCCA+AGG 0.573799 6.4:+44659429 MS.gene59273:CDS
GAAAGTAGGTAAAGGAGAGG+AGG 0.576222 6.4:-44659016 None:intergenic
CTTATGTCTAAAGGAGGAGG+TGG 0.581977 6.4:+44659116 MS.gene59273:CDS
CCACTTATGTCTAAAGGAGG+AGG 0.587624 6.4:+44659113 MS.gene59273:CDS
TCGTTTAAAATTGAGACACT+TGG 0.590571 6.4:+44659395 MS.gene59273:CDS
GATTTCCCTACATCTTGGGT+AGG 0.592043 6.4:+44659341 MS.gene59273:CDS
GGTATAGAGATGAGAATGGA+TGG 0.592483 6.4:+44659489 MS.gene59273:CDS
GCAATATAGTATTTGCTACC+AGG 0.595672 6.4:-44659089 None:intergenic
ATGGAAAGTAGGTAAAGGAG+AGG 0.609367 6.4:-44659019 None:intergenic
CCAATATCACTACAAGCACC+AGG 0.612889 6.4:-44659464 None:intergenic
GCACCACTTATGTCTAAAGG+AGG 0.661209 6.4:+44659110 MS.gene59273:CDS
ATTTGCTACCAGGAAATACG+GGG 0.675453 6.4:-44659079 None:intergenic
GTGCTTGTAGTGATATTGGG+AGG 0.713327 6.4:+44659468 MS.gene59273:CDS
ATATCACTACAAGCACCAGG+TGG 0.748505 6.4:-44659461 None:intergenic
TTTATCATACGAGTAAAACG+TGG 0.798123 6.4:+44659230 MS.gene59273:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGTTCTACAAGATTTTAATG+AGG + chr6.4:44659184-44659203 MS.gene59273:CDS 25.0%
AAATCTTGTAGAACAGTAAC+TGG - chr6.4:44659179-44659198 None:intergenic 30.0%
AACTGAAAATGATCCCTTTA+GGG + chr6.4:44659523-44659542 MS.gene59273:CDS 30.0%
ACTCGTATGATAAATCTAAC+TGG - chr6.4:44659224-44659243 None:intergenic 30.0%
ACTGTTTCAGTAAAAGCTAA+TGG - chr6.4:44659041-44659060 None:intergenic 30.0%
AGTAAAAGCTAATGGAAAGT+AGG - chr6.4:44659033-44659052 None:intergenic 30.0%
ATCAACAAATACAACCCTAA+AGG - chr6.4:44659540-44659559 None:intergenic 30.0%
GAAACAGTTGAAGACATAAA+TGG + chr6.4:44659053-44659072 MS.gene59273:CDS 30.0%
GATAATATTGACGCATTTGA+AGG + chr6.4:44659371-44659390 MS.gene59273:CDS 30.0%
TCAACAAATACAACCCTAAA+GGG - chr6.4:44659539-44659558 None:intergenic 30.0%
TTTATCATACGAGTAAAACG+TGG + chr6.4:44659230-44659249 MS.gene59273:CDS 30.0%
! TAAAGGGATCATTTTCAGTT+GGG - chr6.4:44659523-44659542 None:intergenic 30.0%
! TCGTTTAAAATTGAGACACT+TGG + chr6.4:44659395-44659414 MS.gene59273:CDS 30.0%
!! GATTTTAATGAGGTTGTTAG+AGG + chr6.4:44659194-44659213 MS.gene59273:CDS 30.0%
AAAATTGAGACACTTGGTTC+AGG + chr6.4:44659401-44659420 MS.gene59273:CDS 35.0%
AGCTAATGGAAAGTAGGTAA+AGG - chr6.4:44659027-44659046 None:intergenic 35.0%
ATGATGATTTCCCTACATCT+TGG + chr6.4:44659336-44659355 MS.gene59273:CDS 35.0%
ATTGCACCACTTATGTCTAA+AGG + chr6.4:44659107-44659126 MS.gene59273:CDS 35.0%
CAACTGAAAATGATCCCTTT+AGG + chr6.4:44659522-44659541 MS.gene59273:CDS 35.0%
TGATGATTTCCCTACATCTT+GGG + chr6.4:44659337-44659356 MS.gene59273:CDS 35.0%
! CTAAAGGGATCATTTTCAGT+TGG - chr6.4:44659524-44659543 None:intergenic 35.0%
! GCAATATAGTATTTGCTACC+AGG - chr6.4:44659092-44659111 None:intergenic 35.0%
! GTATTTGCTACCAGGAAATA+CGG - chr6.4:44659084-44659103 None:intergenic 35.0%
! TATTTGCTACCAGGAAATAC+GGG - chr6.4:44659083-44659102 None:intergenic 35.0%
ACAAGCTTGTGTATTGTCCA+AGG + chr6.4:44659429-44659448 MS.gene59273:CDS 40.0%
ATGGAAAGTAGGTAAAGGAG+AGG - chr6.4:44659022-44659041 None:intergenic 40.0%
CAATACCTACCCAAGATGTA+GGG - chr6.4:44659349-44659368 None:intergenic 40.0%
CTGGTGCTTGTAGTGATATT+GGG + chr6.4:44659465-44659484 MS.gene59273:CDS 40.0%
GGATTAAAGCTTGGTAAGAC+AGG + chr6.4:44659140-44659159 MS.gene59273:CDS 40.0%
GGTATAGAGATGAGAATGGA+TGG + chr6.4:44659489-44659508 MS.gene59273:CDS 40.0%
! ATTTGCTACCAGGAAATACG+GGG - chr6.4:44659082-44659101 None:intergenic 40.0%
AATGGAAACCCCGTATTTCC+TGG + chr6.4:44659071-44659090 MS.gene59273:CDS 45.0%
AGATGAGAATGGATGGCGTT+TGG + chr6.4:44659496-44659515 MS.gene59273:CDS 45.0%
AGTGTGCTGAATCTGCCAAA+TGG + chr6.4:44659303-44659322 MS.gene59273:CDS 45.0%
ATATCACTACAAGCACCAGG+TGG - chr6.4:44659464-44659483 None:intergenic 45.0%
ATCATGAGCTAGCACCCATT+TGG - chr6.4:44659321-44659340 None:intergenic 45.0%
CCAATACCTACCCAAGATGT+AGG - chr6.4:44659350-44659369 None:intergenic 45.0%
CCAATATCACTACAAGCACC+AGG - chr6.4:44659467-44659486 None:intergenic 45.0%
CCACTTATGTCTAAAGGAGG+AGG + chr6.4:44659113-44659132 MS.gene59273:CDS 45.0%
CCTACATCTTGGGTAGGTAT+TGG + chr6.4:44659347-44659366 MS.gene59273:CDS 45.0%
CCTCCTCCTTTAGACATAAG+TGG - chr6.4:44659116-44659135 None:intergenic 45.0%
CCTGGTGCTTGTAGTGATAT+TGG + chr6.4:44659464-44659483 MS.gene59273:CDS 45.0%
CTTATGTCTAAAGGAGGAGG+TGG + chr6.4:44659116-44659135 MS.gene59273:CDS 45.0%
GAAAGTAGGTAAAGGAGAGG+AGG - chr6.4:44659019-44659038 None:intergenic 45.0%
GATTTCCCTACATCTTGGGT+AGG + chr6.4:44659341-44659360 MS.gene59273:CDS 45.0%
GCACCACTTATGTCTAAAGG+AGG + chr6.4:44659110-44659129 MS.gene59273:CDS 45.0%
GTGCTTGTAGTGATATTGGG+AGG + chr6.4:44659468-44659487 MS.gene59273:CDS 45.0%
GTGTGCTGAATCTGCCAAAT+GGG + chr6.4:44659304-44659323 MS.gene59273:CDS 45.0%
TTGGCAGATTCAGCACACTT+TGG - chr6.4:44659302-44659321 None:intergenic 45.0%
! GGGAGGTATAGAGATGAGAA+TGG + chr6.4:44659485-44659504 MS.gene59273:CDS 45.0%
AGTAGGTAAAGGAGAGGAGG+AGG - chr6.4:44659016-44659035 None:intergenic 50.0%
ATGTCTAAAGGAGGAGGTGG+TGG + chr6.4:44659119-44659138 MS.gene59273:CDS 50.0%
!! GGAGGTGGTGGATTAAAGCT+TGG + chr6.4:44659131-44659150 MS.gene59273:CDS 50.0%
GGAGAGGAGGAGGAGAGAAA+AGG - chr6.4:44659006-44659025 None:intergenic 55.0%
CCAAGGTTCAGTGCTCCACC+TGG + chr6.4:44659446-44659465 MS.gene59273:CDS 60.0%
!! CCAGGTGGAGCACTGAACCT+TGG - chr6.4:44659449-44659468 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr6.4 gene 44658984 44659589 44658984 ID=MS.gene59273
chr6.4 mRNA 44658984 44659589 44658984 ID=MS.gene59273.t1;Parent=MS.gene59273
chr6.4 exon 44658984 44659589 44658984 ID=MS.gene59273.t1.exon1;Parent=MS.gene59273.t1
chr6.4 CDS 44658984 44659589 44658984 ID=cds.MS.gene59273.t1;Parent=MS.gene59273.t1
Gene Sequence

>MS.gene59273

ATGAAGTCAACATTACTTACCTTTTCTCTCCTCCTCCTCTCCTTTACCTACTTTCCATTAGCTTTTACTGAAACAGTTGAAGACATAAATGGAAACCCCGTATTTCCTGGTAGCAAATACTATATTGCACCACTTATGTCTAAAGGAGGAGGTGGTGGATTAAAGCTTGGTAAGACAGGAAACTCAGAATGTCCAGTTACTGTTCTACAAGATTTTAATGAGGTTGTTAGAGGCTTGCCAGTTAGATTTATCATACGAGTAAAACGTGGTGTTATCTTTACAACTGACGAAGTAGATATTGAGTTTGTTAAGAAACCAAAGTGTGCTGAATCTGCCAAATGGGTGCTAGCTCATGATGATTTCCCTACATCTTGGGTAGGTATTGGTGATAATATTGACGCATTTGAAGGTTCGTTTAAAATTGAGACACTTGGTTCAGGAGCATACAAGCTTGTGTATTGTCCAAGGTTCAGTGCTCCACCTGGTGCTTGTAGTGATATTGGGAGGTATAGAGATGAGAATGGATGGCGTTTGGTCCCAACTGAAAATGATCCCTTTAGGGTTGTATTTGTTGATGCTACTGAATCTGAAAAAGCCGTAGTTTGA

Protein sequence

>MS.gene59273.t1

MKSTLLTFSLLLLSFTYFPLAFTETVEDINGNPVFPGSKYYIAPLMSKGGGGGLKLGKTGNSECPVTVLQDFNEVVRGLPVRFIIRVKRGVIFTTDEVDIEFVKKPKCAESAKWVLAHDDFPTSWVGIGDNIDAFEGSFKIETLGSGAYKLVYCPRFSAPPGACSDIGRYRDENGWRLVPTENDPFRVVFVDATESEKAVV