Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33468.t1 | XP_003604599.2 | 73.9 | 226 | 3 | 2 | 5 | 174 | 463 | 688 | 4.90E-84 | 320.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33468.t1 | O60942 | 38.5 | 122 | 62 | 4 | 31 | 145 | 448 | 563 | 3.6e-11 | 69.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33468.t1 | G7JLW2 | 73.9 | 226 | 3 | 2 | 5 | 174 | 459 | 684 | 3.5e-84 | 320.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene33468 | 0.886467 | 3.33E-72 | -1.69E-46 |
| MS.gene049159 | MS.gene33468 | 0.867611 | 1.20E-65 | -1.69E-46 |
| MS.gene049254 | MS.gene33468 | 0.849233 | 3.69E-60 | -1.69E-46 |
| MS.gene049343 | MS.gene33468 | 0.841581 | 4.36E-58 | -1.69E-46 |
| MS.gene049350 | MS.gene33468 | 0.809838 | 1.55E-50 | -1.69E-46 |
| MS.gene049368 | MS.gene33468 | 0.818951 | 1.50E-52 | -1.69E-46 |
| MS.gene049404 | MS.gene33468 | 0.815055 | 1.12E-51 | -1.69E-46 |
| MS.gene049435 | MS.gene33468 | 0.829215 | 5.83E-55 | -1.69E-46 |
| MS.gene049452 | MS.gene33468 | 0.831369 | 1.74E-55 | -1.69E-46 |
| MS.gene049501 | MS.gene33468 | 0.817055 | 4.02E-52 | -1.69E-46 |
| MS.gene049711 | MS.gene33468 | 0.807932 | 3.97E-50 | -1.69E-46 |
| MS.gene049821 | MS.gene33468 | 0.807471 | 4.97E-50 | -1.69E-46 |
| MS.gene049850 | MS.gene33468 | 0.863019 | 3.35E-64 | -1.69E-46 |
| MS.gene049983 | MS.gene33468 | 0.813851 | 2.08E-51 | -1.69E-46 |
| MS.gene050020 | MS.gene33468 | 0.815008 | 1.15E-51 | -1.69E-46 |
| MS.gene050156 | MS.gene33468 | 0.850502 | 1.63E-60 | -1.69E-46 |
| MS.gene050442 | MS.gene33468 | 0.843622 | 1.25E-58 | -1.69E-46 |
| MS.gene05045 | MS.gene33468 | 0.817374 | 3.41E-52 | -1.69E-46 |
| MS.gene050689 | MS.gene33468 | 0.801698 | 7.96E-49 | -1.69E-46 |
| MS.gene050719 | MS.gene33468 | 0.815843 | 7.51E-52 | -1.69E-46 |
| MS.gene050721 | MS.gene33468 | 0.815065 | 1.12E-51 | -1.69E-46 |
| MS.gene050724 | MS.gene33468 | 0.811561 | 6.58E-51 | -1.69E-46 |
| MS.gene050759 | MS.gene33468 | 0.806526 | 7.88E-50 | -1.69E-46 |
| MS.gene050780 | MS.gene33468 | 0.805499 | 1.30E-49 | -1.69E-46 |
| MS.gene050815 | MS.gene33468 | 0.822968 | 1.78E-53 | -1.69E-46 |
| MS.gene050826 | MS.gene33468 | 0.832308 | 1.02E-55 | -1.69E-46 |
| MS.gene050844 | MS.gene33468 | 0.829406 | 5.24E-55 | -1.69E-46 |
| MS.gene050901 | MS.gene33468 | 0.803471 | 3.43E-49 | -1.69E-46 |
| MS.gene051180 | MS.gene33468 | 0.804499 | 2.10E-49 | -1.69E-46 |
| MS.gene051181 | MS.gene33468 | 0.823346 | 1.46E-53 | -1.69E-46 |
| MS.gene051198 | MS.gene33468 | 0.810509 | 1.11E-50 | -1.69E-46 |
| MS.gene051247 | MS.gene33468 | 0.850055 | 2.17E-60 | -1.69E-46 |
| MS.gene051332 | MS.gene33468 | 0.81199 | 5.31E-51 | -1.69E-46 |
| MS.gene051388 | MS.gene33468 | 0.817822 | 2.70E-52 | -1.69E-46 |
| MS.gene051490 | MS.gene33468 | 0.807134 | 5.86E-50 | -1.69E-46 |
| MS.gene051594 | MS.gene33468 | 0.80431 | 2.30E-49 | -1.69E-46 |
| MS.gene051719 | MS.gene33468 | 0.85535 | 6.69E-62 | -1.69E-46 |
| MS.gene051720 | MS.gene33468 | 0.850628 | 1.50E-60 | -1.69E-46 |
| MS.gene051744 | MS.gene33468 | 0.846858 | 1.67E-59 | -1.69E-46 |
| MS.gene051800 | MS.gene33468 | 0.816937 | 4.27E-52 | -1.69E-46 |
| MS.gene051975 | MS.gene33468 | 0.800479 | 1.41E-48 | -1.69E-46 |
| MS.gene052012 | MS.gene33468 | 0.866356 | 3.03E-65 | -1.69E-46 |
| MS.gene052020 | MS.gene33468 | 0.829082 | 6.28E-55 | -1.69E-46 |
| MS.gene052042 | MS.gene33468 | 0.80845 | 3.08E-50 | -1.69E-46 |
| MS.gene052108 | MS.gene33468 | 0.804848 | 1.77E-49 | -1.69E-46 |
| MS.gene052190 | MS.gene33468 | 0.820115 | 8.13E-53 | -1.69E-46 |
| MS.gene052323 | MS.gene33468 | 0.843448 | 1.39E-58 | -1.69E-46 |
| MS.gene052333 | MS.gene33468 | 0.814732 | 1.33E-51 | -1.69E-46 |
| MS.gene052384 | MS.gene33468 | 0.807228 | 5.60E-50 | -1.69E-46 |
| MS.gene052463 | MS.gene33468 | 0.82608 | 3.30E-54 | -1.69E-46 |
| MS.gene052516 | MS.gene33468 | 0.850536 | 1.59E-60 | -1.69E-46 |
| MS.gene052519 | MS.gene33468 | 0.877915 | 4.28E-69 | -1.69E-46 |
| MS.gene052582 | MS.gene33468 | 0.834068 | 3.71E-56 | -1.69E-46 |
| MS.gene052584 | MS.gene33468 | 0.830408 | 2.99E-55 | -1.69E-46 |
| MS.gene052611 | MS.gene33468 | 0.854499 | 1.18E-61 | -1.69E-46 |
| MS.gene052773 | MS.gene33468 | 0.946452 | 4.49E-105 | -1.69E-46 |
| MS.gene052775 | MS.gene33468 | 0.808083 | 3.69E-50 | -1.69E-46 |
| MS.gene052863 | MS.gene33468 | 0.896602 | 3.15E-76 | -1.69E-46 |
| MS.gene052896 | MS.gene33468 | 0.846099 | 2.69E-59 | -1.69E-46 |
| MS.gene053008 | MS.gene33468 | 0.865243 | 6.80E-65 | -1.69E-46 |
| MS.gene053079 | MS.gene33468 | 0.822859 | 1.89E-53 | -1.69E-46 |
| MS.gene053145 | MS.gene33468 | 0.811434 | 7.01E-51 | -1.69E-46 |
| MS.gene053146 | MS.gene33468 | 0.823586 | 1.28E-53 | -1.69E-46 |
| MS.gene053166 | MS.gene33468 | 0.841379 | 4.93E-58 | -1.69E-46 |
| MS.gene053226 | MS.gene33468 | 0.88072 | 4.35E-70 | -1.69E-46 |
| MS.gene053296 | MS.gene33468 | 0.812808 | 3.52E-51 | -1.69E-46 |
| MS.gene05350 | MS.gene33468 | 0.818134 | 2.30E-52 | -1.69E-46 |
| MS.gene053599 | MS.gene33468 | 0.809652 | 1.70E-50 | -1.69E-46 |
| MS.gene053621 | MS.gene33468 | 0.809174 | 2.16E-50 | -1.69E-46 |
| MS.gene05364 | MS.gene33468 | 0.813917 | 2.01E-51 | -1.69E-46 |
| MS.gene053883 | MS.gene33468 | 0.800378 | 1.48E-48 | -1.69E-46 |
| MS.gene053900 | MS.gene33468 | 0.800193 | 1.62E-48 | -1.69E-46 |
| MS.gene053966 | MS.gene33468 | 0.827192 | 1.79E-54 | -1.69E-46 |
| MS.gene05414 | MS.gene33468 | 0.81985 | 9.35E-53 | -1.69E-46 |
| MS.gene05415 | MS.gene33468 | 0.848329 | 6.57E-60 | -1.69E-46 |
| MS.gene054236 | MS.gene33468 | 0.839582 | 1.45E-57 | -1.69E-46 |
| MS.gene05423 | MS.gene33468 | 0.823217 | 1.56E-53 | -1.69E-46 |
| MS.gene054291 | MS.gene33468 | -0.81848 | 1.92E-52 | -1.69E-46 |
| MS.gene054467 | MS.gene33468 | 0.839731 | 1.33E-57 | -1.69E-46 |
| MS.gene054468 | MS.gene33468 | 0.823364 | 1.44E-53 | -1.69E-46 |
| MS.gene054472 | MS.gene33468 | 0.840285 | 9.54E-58 | -1.69E-46 |
| MS.gene054479 | MS.gene33468 | 0.814567 | 1.44E-51 | -1.69E-46 |
| MS.gene054559 | MS.gene33468 | 0.813621 | 2.33E-51 | -1.69E-46 |
| MS.gene054582 | MS.gene33468 | 0.830187 | 3.38E-55 | -1.69E-46 |
| MS.gene05458 | MS.gene33468 | 0.825447 | 4.67E-54 | -1.69E-46 |
| MS.gene054651 | MS.gene33468 | 0.874152 | 8.44E-68 | -1.69E-46 |
| MS.gene054657 | MS.gene33468 | 0.803202 | 3.90E-49 | -1.69E-46 |
| MS.gene054658 | MS.gene33468 | 0.812773 | 3.58E-51 | -1.69E-46 |
| MS.gene05468 | MS.gene33468 | 0.810552 | 1.09E-50 | -1.69E-46 |
| MS.gene054775 | MS.gene33468 | 0.850082 | 2.14E-60 | -1.69E-46 |
| MS.gene054943 | MS.gene33468 | 0.819344 | 1.22E-52 | -1.69E-46 |
| MS.gene054991 | MS.gene33468 | 0.808469 | 3.05E-50 | -1.69E-46 |
| MS.gene054994 | MS.gene33468 | 0.812601 | 3.90E-51 | -1.69E-46 |
| MS.gene05510 | MS.gene33468 | 0.810279 | 1.25E-50 | -1.69E-46 |
| MS.gene05517 | MS.gene33468 | 0.835989 | 1.22E-56 | -1.69E-46 |
| MS.gene055227 | MS.gene33468 | 0.829955 | 3.85E-55 | -1.69E-46 |
| MS.gene055228 | MS.gene33468 | 0.815394 | 9.46E-52 | -1.69E-46 |
| MS.gene055313 | MS.gene33468 | 0.813161 | 2.94E-51 | -1.69E-46 |
| MS.gene055465 | MS.gene33468 | 0.818094 | 2.34E-52 | -1.69E-46 |
| MS.gene055466 | MS.gene33468 | 0.834445 | 2.99E-56 | -1.69E-46 |
| MS.gene055468 | MS.gene33468 | 0.81297 | 3.24E-51 | -1.69E-46 |
| MS.gene055512 | MS.gene33468 | 0.826932 | 2.07E-54 | -1.69E-46 |
| MS.gene055513 | MS.gene33468 | 0.834451 | 2.97E-56 | -1.69E-46 |
| MS.gene055586 | MS.gene33468 | 0.81062 | 1.05E-50 | -1.69E-46 |
| MS.gene055589 | MS.gene33468 | 0.804224 | 2.39E-49 | -1.69E-46 |
| MS.gene055623 | MS.gene33468 | 0.833625 | 4.79E-56 | -1.69E-46 |
| MS.gene055778 | MS.gene33468 | 0.822961 | 1.79E-53 | -1.69E-46 |
| MS.gene055818 | MS.gene33468 | 0.894313 | 2.77E-75 | -1.69E-46 |
| MS.gene055841 | MS.gene33468 | 0.80209 | 6.62E-49 | -1.69E-46 |
| MS.gene055926 | MS.gene33468 | 0.85083 | 1.32E-60 | -1.69E-46 |
| MS.gene05604 | MS.gene33468 | 0.846168 | 2.57E-59 | -1.69E-46 |
| MS.gene056050 | MS.gene33468 | 0.845289 | 4.46E-59 | -1.69E-46 |
| MS.gene056162 | MS.gene33468 | 0.800672 | 1.29E-48 | -1.69E-46 |
| MS.gene056173 | MS.gene33468 | 0.860493 | 1.99E-63 | -1.69E-46 |
| MS.gene056174 | MS.gene33468 | 0.846044 | 2.78E-59 | -1.69E-46 |
| MS.gene056181 | MS.gene33468 | 0.802717 | 4.91E-49 | -1.69E-46 |
| MS.gene056215 | MS.gene33468 | 0.808486 | 3.03E-50 | -1.69E-46 |
| MS.gene056308 | MS.gene33468 | 0.828656 | 7.96E-55 | -1.69E-46 |
| MS.gene056366 | MS.gene33468 | 0.857388 | 1.69E-62 | -1.69E-46 |
| MS.gene056373 | MS.gene33468 | 0.801785 | 7.64E-49 | -1.69E-46 |
| MS.gene056498 | MS.gene33468 | 0.807102 | 5.95E-50 | -1.69E-46 |
| MS.gene056506 | MS.gene33468 | 0.818765 | 1.65E-52 | -1.69E-46 |
| MS.gene056595 | MS.gene33468 | 0.839076 | 1.97E-57 | -1.69E-46 |
| MS.gene056699 | MS.gene33468 | 0.815153 | 1.07E-51 | -1.69E-46 |
| MS.gene056776 | MS.gene33468 | 0.814881 | 1.23E-51 | -1.69E-46 |
| MS.gene05686 | MS.gene33468 | 0.821077 | 4.89E-53 | -1.69E-46 |
| MS.gene05690 | MS.gene33468 | 0.834095 | 3.65E-56 | -1.69E-46 |
| MS.gene057026 | MS.gene33468 | 0.839872 | 1.22E-57 | -1.69E-46 |
| MS.gene057034 | MS.gene33468 | 0.824235 | 9.01E-54 | -1.69E-46 |
| MS.gene057060 | MS.gene33468 | 0.813841 | 2.09E-51 | -1.69E-46 |
| MS.gene057208 | MS.gene33468 | 0.845334 | 4.33E-59 | -1.69E-46 |
| MS.gene057444 | MS.gene33468 | 0.836917 | 7.07E-57 | -1.69E-46 |
| MS.gene057598 | MS.gene33468 | 0.848503 | 5.88E-60 | -1.69E-46 |
| MS.gene057693 | MS.gene33468 | 0.849712 | 2.71E-60 | -1.69E-46 |
| MS.gene057855 | MS.gene33468 | 0.868553 | 5.99E-66 | -1.69E-46 |
| MS.gene057869 | MS.gene33468 | 0.835644 | 1.49E-56 | -1.69E-46 |
| MS.gene057915 | MS.gene33468 | 0.865854 | 4.36E-65 | -1.69E-46 |
| MS.gene058008 | MS.gene33468 | 0.812421 | 4.28E-51 | -1.69E-46 |
| MS.gene058009 | MS.gene33468 | 0.840308 | 9.41E-58 | -1.69E-46 |
| MS.gene058010 | MS.gene33468 | 0.851163 | 1.06E-60 | -1.69E-46 |
| MS.gene058037 | MS.gene33468 | 0.815996 | 6.94E-52 | -1.69E-46 |
| MS.gene058146 | MS.gene33468 | 0.81053 | 1.10E-50 | -1.69E-46 |
| MS.gene058355 | MS.gene33468 | 0.800346 | 1.50E-48 | -1.69E-46 |
| MS.gene05847 | MS.gene33468 | 0.813012 | 3.17E-51 | -1.69E-46 |
| MS.gene058625 | MS.gene33468 | 0.864671 | 1.03E-64 | -1.69E-46 |
| MS.gene058636 | MS.gene33468 | 0.844976 | 5.41E-59 | -1.69E-46 |
| MS.gene058783 | MS.gene33468 | 0.818944 | 1.50E-52 | -1.69E-46 |
| MS.gene058829 | MS.gene33468 | 0.835177 | 1.95E-56 | -1.69E-46 |
| MS.gene058979 | MS.gene33468 | 0.866176 | 3.45E-65 | -1.69E-46 |
| MS.gene059283 | MS.gene33468 | 0.833333 | 5.66E-56 | -1.69E-46 |
| MS.gene059439 | MS.gene33468 | 0.808247 | 3.40E-50 | -1.69E-46 |
| MS.gene05950 | MS.gene33468 | 0.81128 | 7.57E-51 | -1.69E-46 |
| MS.gene059570 | MS.gene33468 | 0.840259 | 9.68E-58 | -1.69E-46 |
| MS.gene059625 | MS.gene33468 | 0.820315 | 7.32E-53 | -1.69E-46 |
| MS.gene059725 | MS.gene33468 | 0.827336 | 1.66E-54 | -1.69E-46 |
| MS.gene059842 | MS.gene33468 | 0.830937 | 2.22E-55 | -1.69E-46 |
| MS.gene059937 | MS.gene33468 | 0.817235 | 3.66E-52 | -1.69E-46 |
| MS.gene059965 | MS.gene33468 | 0.806327 | 8.68E-50 | -1.69E-46 |
| MS.gene060011 | MS.gene33468 | 0.807006 | 6.24E-50 | -1.69E-46 |
| MS.gene060310 | MS.gene33468 | 0.815598 | 8.52E-52 | -1.69E-46 |
| MS.gene060322 | MS.gene33468 | 0.851157 | 1.07E-60 | -1.69E-46 |
| MS.gene060365 | MS.gene33468 | 0.802402 | 5.71E-49 | -1.69E-46 |
| MS.gene060429 | MS.gene33468 | 0.801189 | 1.01E-48 | -1.69E-46 |
| MS.gene06052 | MS.gene33468 | 0.833806 | 4.32E-56 | -1.69E-46 |
| MS.gene060572 | MS.gene33468 | 0.830012 | 3.73E-55 | -1.69E-46 |
| MS.gene060685 | MS.gene33468 | 0.808108 | 3.64E-50 | -1.69E-46 |
| MS.gene060802 | MS.gene33468 | 0.819508 | 1.12E-52 | -1.69E-46 |
| MS.gene060803 | MS.gene33468 | 0.878088 | 3.73E-69 | -1.69E-46 |
| MS.gene060935 | MS.gene33468 | 0.828857 | 7.12E-55 | -1.69E-46 |
| MS.gene060937 | MS.gene33468 | 0.808323 | 3.28E-50 | -1.69E-46 |
| MS.gene060998 | MS.gene33468 | 0.818137 | 2.29E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33468.t1 | MTR_4g014920 | 73.894 | 226 | 3 | 2 | 5 | 174 | 463 | 688 | 2.99e-107 | 321 |
| MS.gene33468.t1 | MTR_4g014920 | 73.894 | 226 | 3 | 2 | 5 | 174 | 459 | 684 | 3.25e-107 | 321 |
| MS.gene33468.t1 | MTR_7g066810 | 56.944 | 216 | 40 | 2 | 5 | 167 | 472 | 687 | 7.08e-74 | 234 |
| MS.gene33468.t1 | MTR_4g014920 | 67.039 | 179 | 3 | 2 | 5 | 127 | 463 | 641 | 2.36e-72 | 229 |
| MS.gene33468.t1 | MTR_7g094670 | 53.744 | 227 | 46 | 4 | 5 | 174 | 408 | 632 | 2.16e-67 | 222 |
| MS.gene33468.t1 | MTR_7g068260 | 63.810 | 105 | 33 | 2 | 7 | 107 | 29 | 132 | 1.64e-39 | 131 |
| MS.gene33468.t1 | MTR_4g014920 | 45.370 | 108 | 3 | 2 | 5 | 56 | 463 | 570 | 1.24e-18 | 82.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33468.t1 | AT3G09100 | 55.140 | 214 | 44 | 3 | 2 | 163 | 455 | 668 | 4.46e-69 | 221 |
| MS.gene33468.t1 | AT5G01290 | 66.187 | 139 | 45 | 1 | 27 | 163 | 515 | 653 | 2.80e-59 | 195 |
| MS.gene33468.t1 | AT5G28210 | 37.615 | 218 | 77 | 6 | 2 | 162 | 398 | 613 | 6.96e-35 | 129 |
Find 34 sgRNAs with CRISPR-Local
Find 104 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGGTTAATGGCTCAAATTT+TGG | 0.268457 | 4.4:-86022560 | None:intergenic |
| CATCTTCCATCTTTCATAGA+AGG | 0.284381 | 4.4:+86022528 | None:intergenic |
| AAAGTCAACTGAATTTAAAT+TGG | 0.286326 | 4.4:+86022243 | None:intergenic |
| GAAACTCTTGGATGGGAATA+AGG | 0.299659 | 4.4:-86022058 | MS.gene33468:CDS |
| CTGTAAGTATTAAACTCATT+TGG | 0.310885 | 4.4:+86021196 | None:intergenic |
| AGAACACTCGATGATCTTTC+CGG | 0.326134 | 4.4:+86021276 | None:intergenic |
| TTCAGTTGACTTTCTGTTTG+AGG | 0.348533 | 4.4:-86022233 | MS.gene33468:intron |
| GAAAGATCATCGAGTGTTCT+TGG | 0.397309 | 4.4:-86021273 | MS.gene33468:CDS |
| TTCGCAGGAATATCCATTCC+AGG | 0.421148 | 4.4:+86021239 | None:intergenic |
| GAATCACCTTCGCTGCTTTG+CGG | 0.427455 | 4.4:+86020962 | None:intergenic |
| TGCTTCCATCGGCAGGTTGA+AGG | 0.440873 | 4.4:-86022120 | MS.gene33468:intron |
| AAAGATCATCGAGTGTTCTT+GGG | 0.450552 | 4.4:-86021272 | MS.gene33468:CDS |
| TCATGAGTACGTGGTACATA+AGG | 0.478867 | 4.4:+86022289 | None:intergenic |
| GGGAATAAGGTTGCTTTCAA+AGG | 0.488951 | 4.4:-86022045 | MS.gene33468:intron |
| ATTTATCTGTTCTAATTCGC+AGG | 0.489727 | 4.4:+86021224 | None:intergenic |
| ATCGGCAGGTTGAAGGTGAT+CGG | 0.499299 | 4.4:-86022113 | MS.gene33468:intron |
| GATCTTTCCGGAATAAAATG+AGG | 0.503180 | 4.4:+86021288 | None:intergenic |
| TCGGCAGGTTGAAGGTGATC+GGG | 0.508639 | 4.4:-86022112 | MS.gene33468:intron |
| ATCTTCCATCTTTCATAGAA+GGG | 0.521972 | 4.4:+86022529 | None:intergenic |
| CTCATGAAGGACTCTTAAAG+TGG | 0.522723 | 4.4:-86022273 | MS.gene33468:CDS |
| CTCATGCCGCAAAGCAGCGA+AGG | 0.526270 | 4.4:-86020968 | MS.gene33468:CDS |
| TGAGTTTAATACTTACAGAA+AGG | 0.527213 | 4.4:-86021191 | MS.gene33468:intron |
| GCCTTCCCATGTATGCGGAC+AGG | 0.528594 | 4.4:-86021022 | MS.gene33468:CDS |
| GCGGGGAAAGAAGAAACTCT+TGG | 0.535079 | 4.4:-86022070 | MS.gene33468:CDS |
| GGAAAGAAGAAACTCTTGGA+TGG | 0.552512 | 4.4:-86022066 | MS.gene33468:CDS |
| TCCTGTCCGCATACATGGGA+AGG | 0.564771 | 4.4:+86021021 | None:intergenic |
| AGCGACCCTTCTATGAAAGA+TGG | 0.580254 | 4.4:-86022534 | MS.gene33468:CDS |
| TATGTACCACGTACTCATGA+AGG | 0.583824 | 4.4:-86022286 | MS.gene33468:CDS |
| GAAAGAAGAAACTCTTGGAT+GGG | 0.603849 | 4.4:-86022065 | MS.gene33468:CDS |
| ATCTTTCCGGAATAAAATGA+GGG | 0.606895 | 4.4:+86021289 | None:intergenic |
| TTTATCCTGTCCGCATACAT+GGG | 0.615995 | 4.4:+86021017 | None:intergenic |
| AATTCGCCTTCCCATGTATG+CGG | 0.681083 | 4.4:-86021027 | MS.gene33468:CDS |
| AAGAGTCCTTCATGAGTACG+TGG | 0.699809 | 4.4:+86022280 | None:intergenic |
| CATAAAAGACAACATCACAG+AGG | 0.751598 | 4.4:-86021078 | MS.gene33468:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATATTTTTGAAATGTTTTTT+AGG | - | chr4.4:86021180-86021199 | MS.gene33468:intron | 10.0% |
| !! | AGATAAAAGAAACTAAAAAT+TGG | + | chr4.4:86022367-86022386 | None:intergenic | 15.0% |
| !! | ATATGATTATTTAAATGAGT+CGG | + | chr4.4:86021863-86021882 | None:intergenic | 15.0% |
| !! | CATAATTATAAAAACAAAGT+TGG | + | chr4.4:86021527-86021546 | None:intergenic | 15.0% |
| !! | GATAAAAGAAACTAAAAATT+GGG | + | chr4.4:86022366-86022385 | None:intergenic | 15.0% |
| !! | TTCCATTTATATTGAATAAA+AGG | - | chr4.4:86021761-86021780 | MS.gene33468:intron | 15.0% |
| !!! | TACCTTTTATTCAATATAAA+TGG | + | chr4.4:86021766-86021785 | None:intergenic | 15.0% |
| !! | AAAGTCAACTGAATTTAAAT+TGG | + | chr4.4:86021278-86021297 | None:intergenic | 20.0% |
| !! | AATTAAACAAGCCAAAATAA+AGG | - | chr4.4:86021669-86021688 | MS.gene33468:intron | 20.0% |
| !! | ATTAAACAAGCCAAAATAAA+GGG | - | chr4.4:86021670-86021689 | MS.gene33468:intron | 20.0% |
| !! | CACCTATAAAATTAAGTAAA+TGG | + | chr4.4:86022412-86022431 | None:intergenic | 20.0% |
| !! | TATGATTATTTAAATGAGTC+GGG | + | chr4.4:86021862-86021881 | None:intergenic | 20.0% |
| !! | TGATTAAATAATTCGTGAAT+AGG | - | chr4.4:86021947-86021966 | MS.gene33468:intron | 20.0% |
| !!! | AATAAAAGGTATAATTCTCT+AGG | - | chr4.4:86021775-86021794 | MS.gene33468:intron | 20.0% |
| !!! | AGCCATTTACTTAATTTTAT+AGG | - | chr4.4:86022407-86022426 | MS.gene33468:intron | 20.0% |
| !!! | TTGGAATTTTTGATATTACT+TGG | - | chr4.4:86021336-86021355 | MS.gene33468:intron | 20.0% |
| !!! | TTTGAAATGTTTTTTAGGTA+AGG | - | chr4.4:86021185-86021204 | MS.gene33468:intron | 20.0% |
| ! | AATTGCTTGAATTTATAACC+TGG | - | chr4.4:86021613-86021632 | MS.gene33468:intron | 25.0% |
| ! | ATAAAACAAACTATGAACTG+TGG | + | chr4.4:86022150-86022169 | None:intergenic | 25.0% |
| ! | CTGTAAGTATTAAACTCATT+TGG | + | chr4.4:86022325-86022344 | None:intergenic | 25.0% |
| ! | TAAAAATTGGGAGTTAGTAT+TGG | + | chr4.4:86022354-86022373 | None:intergenic | 25.0% |
| ! | TGAGTTTAATACTTACAGAA+AGG | - | chr4.4:86022327-86022346 | MS.gene33468:intron | 25.0% |
| ! | TTTATCTCTAAATCTGTACT+TGG | - | chr4.4:86022380-86022399 | MS.gene33468:intron | 25.0% |
| AGTATGTTGTGATCAATGTA+AGG | + | chr4.4:86021126-86021145 | None:intergenic | 30.0% | |
| ATAGGAAATTTAGACAGTCA+TGG | - | chr4.4:86021562-86021581 | MS.gene33468:intron | 30.0% | |
| ATCTTCCATCTTTCATAGAA+GGG | + | chr4.4:86020992-86021011 | None:intergenic | 30.0% | |
| ATCTTTCCGGAATAAAATGA+GGG | + | chr4.4:86022232-86022251 | None:intergenic | 30.0% | |
| ATTTATCTGTTCTAATTCGC+AGG | + | chr4.4:86022297-86022316 | None:intergenic | 30.0% | |
| GACTTTAATAACTGAGACAT+GGG | + | chr4.4:86021827-86021846 | None:intergenic | 30.0% | |
| TAAGCCATAAGAGAAAAATG+AGG | + | chr4.4:86021154-86021173 | None:intergenic | 30.0% | |
| TGACTTTAATAACTGAGACA+TGG | + | chr4.4:86021828-86021847 | None:intergenic | 30.0% | |
| ! | CATTTTTCTCTTATGGCTTA+CGG | - | chr4.4:86021154-86021173 | MS.gene33468:intron | 30.0% |
| ! | TATCATCACCTTCTTTTGTA+TGG | - | chr4.4:86021309-86021328 | MS.gene33468:intron | 30.0% |
| !!! | GAAATGTTTTTTAGGTAAGG+AGG | - | chr4.4:86021188-86021207 | MS.gene33468:intron | 30.0% |
| !!! | TAATTTTTAGCTGCTTCCAT+CGG | - | chr4.4:86021387-86021406 | MS.gene33468:intron | 30.0% |
| AAAGATCATCGAGTGTTCTT+GGG | - | chr4.4:86022246-86022265 | MS.gene33468:CDS | 35.0% | |
| AGAAAGAAGTGATTGAACCT+AGG | - | chr4.4:86021017-86021036 | MS.gene33468:CDS | 35.0% | |
| ATGTTGTGTGTCTGTAGAAT+AGG | - | chr4.4:86021544-86021563 | MS.gene33468:intron | 35.0% | |
| ATTTAGACAGTCATGGCATT+TGG | - | chr4.4:86021569-86021588 | MS.gene33468:intron | 35.0% | |
| CACTTGCTTTAATTGCTTTC+TGG | - | chr4.4:86022178-86022197 | MS.gene33468:intron | 35.0% | |
| CATAAAAGACAACATCACAG+AGG | - | chr4.4:86022440-86022459 | MS.gene33468:intron | 35.0% | |
| CATCTTCCATCTTTCATAGA+AGG | + | chr4.4:86020993-86021012 | None:intergenic | 35.0% | |
| CCAAAACACCATACAAAAGA+AGG | + | chr4.4:86021320-86021339 | None:intergenic | 35.0% | |
| GAAAGAAGAAACTCTTGGAT+GGG | - | chr4.4:86021453-86021472 | MS.gene33468:intron | 35.0% | |
| GATCTTTCCGGAATAAAATG+AGG | + | chr4.4:86022233-86022252 | None:intergenic | 35.0% | |
| GGAAGCAGCTAAAAATTAGA+TGG | + | chr4.4:86021385-86021404 | None:intergenic | 35.0% | |
| GGTAACATTAAATCTGCAGA+TGG | - | chr4.4:86022199-86022218 | MS.gene33468:intron | 35.0% | |
| GGTCAGTTGTTTGATATTGA+TGG | - | chr4.4:86021357-86021376 | MS.gene33468:intron | 35.0% | |
| TCCAGATCATATCTGTAGTA+AGG | + | chr4.4:86021079-86021098 | None:intergenic | 35.0% | |
| TCCTTACTACAGATATGATC+TGG | - | chr4.4:86021075-86021094 | MS.gene33468:CDS | 35.0% | |
| TTTAGACAGTCATGGCATTT+GGG | - | chr4.4:86021570-86021589 | MS.gene33468:intron | 35.0% | |
| ! | ACAGAACCCTCATTTTATTC+CGG | - | chr4.4:86022223-86022242 | MS.gene33468:intron | 35.0% |
| ! | AGGTAAGGAGGAAAGATTTT+TGG | - | chr4.4:86021200-86021219 | MS.gene33468:CDS | 35.0% |
| ! | ATATGATCTGGAACCATTCA+GGG | - | chr4.4:86021087-86021106 | MS.gene33468:CDS | 35.0% |
| ! | CCTTCTTTTGTATGGTGTTT+TGG | - | chr4.4:86021317-86021336 | MS.gene33468:intron | 35.0% |
| ! | GGTAAGGAGGAAAGATTTTT+GGG | - | chr4.4:86021201-86021220 | MS.gene33468:CDS | 35.0% |
| ! | TTCAGTTGACTTTCTGTTTG+AGG | - | chr4.4:86021285-86021304 | MS.gene33468:CDS | 35.0% |
| !! | GAAGCCTCATTTTTCTCTTA+TGG | - | chr4.4:86021147-86021166 | MS.gene33468:intron | 35.0% |
| !! | GCATGGCTTTAGTTTACTTA+TGG | - | chr4.4:86021726-86021745 | MS.gene33468:intron | 35.0% |
| !! | TTCTTGGGATTTTGATAACC+TGG | - | chr4.4:86022261-86022280 | MS.gene33468:CDS | 35.0% |
| !!! | AGCTACTTTTTGTGTATGAG+CGG | - | chr4.4:86021429-86021448 | MS.gene33468:intron | 35.0% |
| AGAACACTCGATGATCTTTC+CGG | + | chr4.4:86022245-86022264 | None:intergenic | 40.0% | |
| CAAAATAAAGGGTTTGCGCT+TGG | - | chr4.4:86021681-86021700 | MS.gene33468:intron | 40.0% | |
| CTCAAGCCTCAAATTCAAAC+AGG | + | chr4.4:86021707-86021726 | None:intergenic | 40.0% | |
| CTCATGAAGGACTCTTAAAG+TGG | - | chr4.4:86021245-86021264 | MS.gene33468:CDS | 40.0% | |
| GAAACTCTTGGATGGGAATA+AGG | - | chr4.4:86021460-86021479 | MS.gene33468:intron | 40.0% | |
| GAAAGATCATCGAGTGTTCT+TGG | - | chr4.4:86022245-86022264 | MS.gene33468:CDS | 40.0% | |
| GGAAAGAAGAAACTCTTGGA+TGG | - | chr4.4:86021452-86021471 | MS.gene33468:intron | 40.0% | |
| TATGTACCACGTACTCATGA+AGG | - | chr4.4:86021232-86021251 | MS.gene33468:CDS | 40.0% | |
| TCATGAGTACGTGGTACATA+AGG | + | chr4.4:86021232-86021251 | None:intergenic | 40.0% | |
| TGTAGTAAGGATTTCTGCTC+TGG | + | chr4.4:86021066-86021085 | None:intergenic | 40.0% | |
| TTTAGTGCCCACCAAAAAGT+GGG | - | chr4.4:86022035-86022054 | MS.gene33468:intron | 40.0% | |
| TTTATCCTGTCCGCATACAT+GGG | + | chr4.4:86022504-86022523 | None:intergenic | 40.0% | |
| ! | GATATGATCTGGAACCATTC+AGG | - | chr4.4:86021086-86021105 | MS.gene33468:CDS | 40.0% |
| ! | GGGAATAAGGTTGCTTTCAA+AGG | - | chr4.4:86021473-86021492 | MS.gene33468:intron | 40.0% |
| ! | TAAGGTTGCTTTCAAAGGTC+AGG | - | chr4.4:86021478-86021497 | MS.gene33468:intron | 40.0% |
| ! | TTGAATTTGAGGCTTGAGCA+TGG | - | chr4.4:86021709-86021728 | MS.gene33468:intron | 40.0% |
| ! | TTTTATCCTGTCCGCATACA+TGG | + | chr4.4:86022505-86022524 | None:intergenic | 40.0% |
| !! | ATCCTTCTCAACCCACTTTT+TGG | + | chr4.4:86022049-86022068 | None:intergenic | 40.0% |
| !! | CAAGCGCAAACCCTTTATTT+TGG | + | chr4.4:86021683-86021702 | None:intergenic | 40.0% |
| !! | GAACATCATTTTTGCAAGCC+AGG | + | chr4.4:86021634-86021653 | None:intergenic | 40.0% |
| !! | GGGATTTTGATAACCTGGAA+TGG | - | chr4.4:86022266-86022285 | MS.gene33468:CDS | 40.0% |
| !!! | CTACTTTTTGTGTATGAGCG+GGG | - | chr4.4:86021431-86021450 | MS.gene33468:intron | 40.0% |
| !!! | GCTACTTTTTGTGTATGAGC+GGG | - | chr4.4:86021430-86021449 | MS.gene33468:intron | 40.0% |
| !!! | TTCTCAACCCACTTTTTGGT+GGG | + | chr4.4:86022045-86022064 | None:intergenic | 40.0% |
| AAGAGTCCTTCATGAGTACG+TGG | + | chr4.4:86021241-86021260 | None:intergenic | 45.0% | |
| AATTCGCCTTCCCATGTATG+CGG | - | chr4.4:86022491-86022510 | MS.gene33468:intron | 45.0% | |
| AGCGACCCTTCTATGAAAGA+TGG | - | chr4.4:86020984-86021003 | MS.gene33468:CDS | 45.0% | |
| CTTTAGTGCCCACCAAAAAG+TGG | - | chr4.4:86022034-86022053 | MS.gene33468:intron | 45.0% | |
| GCTTGGCCTGTTTGAATTTG+AGG | - | chr4.4:86021698-86021717 | MS.gene33468:intron | 45.0% | |
| TGATGTCGTTCGTGATTCCT+AGG | + | chr4.4:86021037-86021056 | None:intergenic | 45.0% | |
| TTCAAGATGCATGCCCTGAA+TGG | + | chr4.4:86021103-86021122 | None:intergenic | 45.0% | |
| TTCGCAGGAATATCCATTCC+AGG | + | chr4.4:86022282-86022301 | None:intergenic | 45.0% | |
| !! | CACCAAAAAGTGGGTTGAGA+AGG | - | chr4.4:86022044-86022063 | MS.gene33468:intron | 45.0% |
| !!! | CTTCTCAACCCACTTTTTGG+TGG | + | chr4.4:86022046-86022065 | None:intergenic | 45.0% |
| GCGGGGAAAGAAGAAACTCT+TGG | - | chr4.4:86021448-86021467 | MS.gene33468:intron | 50.0% | |
| ! | ATCGGCAGGTTGAAGGTGAT+CGG | - | chr4.4:86021405-86021424 | MS.gene33468:intron | 50.0% |
| !! | TTTTAGCTGCTTCCATCGGC+AGG | - | chr4.4:86021391-86021410 | MS.gene33468:intron | 50.0% |
| GATCACCTTCAACCTGCCGA+TGG | + | chr4.4:86021406-86021425 | None:intergenic | 55.0% | |
| TCCTGTCCGCATACATGGGA+AGG | + | chr4.4:86022500-86022519 | None:intergenic | 55.0% | |
| ! | TCGGCAGGTTGAAGGTGATC+GGG | - | chr4.4:86021406-86021425 | MS.gene33468:intron | 55.0% |
| ! | TGCTTCCATCGGCAGGTTGA+AGG | - | chr4.4:86021398-86021417 | MS.gene33468:intron | 55.0% |
| CTCATGCCGCAAAGCAGCGA+AGG | - | chr4.4:86022550-86022569 | MS.gene33468:CDS | 60.0% | |
| GCCTTCCCATGTATGCGGAC+AGG | - | chr4.4:86022496-86022515 | MS.gene33468:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 86020965 | 86022575 | 86020965 | ID=MS.gene33468 |
| chr4.4 | mRNA | 86020965 | 86022575 | 86020965 | ID=MS.gene33468.t1;Parent=MS.gene33468 |
| chr4.4 | exon | 86022516 | 86022575 | 86022516 | ID=MS.gene33468.t1.exon1;Parent=MS.gene33468.t1 |
| chr4.4 | CDS | 86022516 | 86022575 | 86022516 | ID=cds.MS.gene33468.t1;Parent=MS.gene33468.t1 |
| chr4.4 | exon | 86022234 | 86022338 | 86022234 | ID=MS.gene33468.t1.exon2;Parent=MS.gene33468.t1 |
| chr4.4 | CDS | 86022234 | 86022338 | 86022234 | ID=cds.MS.gene33468.t1;Parent=MS.gene33468.t1 |
| chr4.4 | exon | 86022046 | 86022127 | 86022046 | ID=MS.gene33468.t1.exon3;Parent=MS.gene33468.t1 |
| chr4.4 | CDS | 86022046 | 86022127 | 86022046 | ID=cds.MS.gene33468.t1;Parent=MS.gene33468.t1 |
| chr4.4 | exon | 86021192 | 86021322 | 86021192 | ID=MS.gene33468.t1.exon4;Parent=MS.gene33468.t1 |
| chr4.4 | CDS | 86021192 | 86021322 | 86021192 | ID=cds.MS.gene33468.t1;Parent=MS.gene33468.t1 |
| chr4.4 | exon | 86020965 | 86021111 | 86020965 | ID=MS.gene33468.t1.exon5;Parent=MS.gene33468.t1 |
| chr4.4 | CDS | 86020965 | 86021111 | 86020965 | ID=cds.MS.gene33468.t1;Parent=MS.gene33468.t1 |
| Gene Sequence |
| Protein sequence |
