Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074606.t1 | XP_013458671.1 | 100 | 59 | 0 | 0 | 69 | 127 | 648 | 706 | 3.20E-24 | 121.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074606.t1 | Q53RK8 | 80.3 | 61 | 12 | 0 | 66 | 126 | 674 | 734 | 5.2e-23 | 108.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074606.t1 | A0A072UUE3 | 100.0 | 59 | 0 | 0 | 69 | 127 | 648 | 706 | 2.3e-24 | 121.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049159 | MS.gene074606 | 0.834959 | 2.22E-56 | -1.69E-46 |
| MS.gene049254 | MS.gene074606 | 0.808794 | 2.60E-50 | -1.69E-46 |
| MS.gene049255 | MS.gene074606 | 0.832414 | 9.58E-56 | -1.69E-46 |
| MS.gene049343 | MS.gene074606 | 0.829346 | 5.42E-55 | -1.69E-46 |
| MS.gene049452 | MS.gene074606 | 0.805202 | 1.50E-49 | -1.69E-46 |
| MS.gene049821 | MS.gene074606 | 0.806171 | 9.37E-50 | -1.69E-46 |
| MS.gene049825 | MS.gene074606 | 0.815458 | 9.15E-52 | -1.69E-46 |
| MS.gene049873 | MS.gene074606 | 0.844765 | 6.17E-59 | -1.69E-46 |
| MS.gene050355 | MS.gene074606 | 0.812373 | 4.38E-51 | -1.69E-46 |
| MS.gene050689 | MS.gene074606 | 0.814733 | 1.33E-51 | -1.69E-46 |
| MS.gene050799 | MS.gene074606 | 0.813602 | 2.36E-51 | -1.69E-46 |
| MS.gene050815 | MS.gene074606 | 0.817443 | 3.29E-52 | -1.69E-46 |
| MS.gene050905 | MS.gene074606 | -0.802799 | 4.73E-49 | -1.69E-46 |
| MS.gene051003 | MS.gene074606 | 0.802202 | 6.27E-49 | -1.69E-46 |
| MS.gene051241 | MS.gene074606 | 0.813896 | 2.03E-51 | -1.69E-46 |
| MS.gene051243 | MS.gene074606 | 0.80569 | 1.18E-49 | -1.69E-46 |
| MS.gene051328 | MS.gene074606 | 0.830695 | 2.54E-55 | -1.69E-46 |
| MS.gene05134 | MS.gene074606 | 0.850951 | 1.22E-60 | -1.69E-46 |
| MS.gene051363 | MS.gene074606 | 0.821888 | 3.18E-53 | -1.69E-46 |
| MS.gene051744 | MS.gene074606 | 0.803129 | 4.04E-49 | -1.69E-46 |
| MS.gene05188 | MS.gene074606 | 0.8035 | 3.38E-49 | -1.69E-46 |
| MS.gene052012 | MS.gene074606 | 0.802254 | 6.12E-49 | -1.69E-46 |
| MS.gene052516 | MS.gene074606 | 0.812761 | 3.60E-51 | -1.69E-46 |
| MS.gene052519 | MS.gene074606 | 0.891961 | 2.46E-74 | -1.69E-46 |
| MS.gene052582 | MS.gene074606 | 0.802519 | 5.40E-49 | -1.69E-46 |
| MS.gene052584 | MS.gene074606 | 0.81909 | 1.39E-52 | -1.69E-46 |
| MS.gene052611 | MS.gene074606 | 0.800824 | 1.20E-48 | -1.69E-46 |
| MS.gene052773 | MS.gene074606 | 0.818671 | 1.74E-52 | -1.69E-46 |
| MS.gene052863 | MS.gene074606 | 0.849737 | 2.67E-60 | -1.69E-46 |
| MS.gene053006 | MS.gene074606 | 0.821448 | 4.02E-53 | -1.69E-46 |
| MS.gene053008 | MS.gene074606 | 0.861451 | 1.02E-63 | -1.69E-46 |
| MS.gene053145 | MS.gene074606 | 0.826887 | 2.12E-54 | -1.69E-46 |
| MS.gene053146 | MS.gene074606 | 0.80388 | 2.82E-49 | -1.69E-46 |
| MS.gene053226 | MS.gene074606 | 0.843215 | 1.61E-58 | -1.69E-46 |
| MS.gene05364 | MS.gene074606 | 0.812131 | 4.95E-51 | -1.69E-46 |
| MS.gene053931 | MS.gene074606 | 0.824061 | 9.90E-54 | -1.69E-46 |
| MS.gene054236 | MS.gene074606 | 0.80455 | 2.05E-49 | -1.69E-46 |
| MS.gene054582 | MS.gene074606 | 0.80553 | 1.28E-49 | -1.69E-46 |
| MS.gene05458 | MS.gene074606 | 0.801605 | 8.32E-49 | -1.69E-46 |
| MS.gene054651 | MS.gene074606 | 0.845242 | 4.59E-59 | -1.69E-46 |
| MS.gene054657 | MS.gene074606 | 0.813228 | 2.85E-51 | -1.69E-46 |
| MS.gene054775 | MS.gene074606 | 0.808253 | 3.39E-50 | -1.69E-46 |
| MS.gene054994 | MS.gene074606 | 0.810224 | 1.28E-50 | -1.69E-46 |
| MS.gene05517 | MS.gene074606 | 0.834893 | 2.30E-56 | -1.69E-46 |
| MS.gene055512 | MS.gene074606 | 0.807637 | 4.59E-50 | -1.69E-46 |
| MS.gene055555 | MS.gene074606 | 0.813534 | 2.44E-51 | -1.69E-46 |
| MS.gene055818 | MS.gene074606 | 0.848354 | 6.47E-60 | -1.69E-46 |
| MS.gene055841 | MS.gene074606 | 0.834179 | 3.48E-56 | -1.69E-46 |
| MS.gene056215 | MS.gene074606 | 0.833142 | 6.32E-56 | -1.69E-46 |
| MS.gene056366 | MS.gene074606 | 0.828953 | 6.75E-55 | -1.69E-46 |
| MS.gene056595 | MS.gene074606 | 0.845212 | 4.68E-59 | -1.69E-46 |
| MS.gene056642 | MS.gene074606 | 0.807095 | 5.98E-50 | -1.69E-46 |
| MS.gene05690 | MS.gene074606 | 0.801926 | 7.15E-49 | -1.69E-46 |
| MS.gene057060 | MS.gene074606 | 0.821266 | 4.42E-53 | -1.69E-46 |
| MS.gene057140 | MS.gene074606 | 0.802031 | 6.80E-49 | -1.69E-46 |
| MS.gene057208 | MS.gene074606 | 0.869982 | 2.06E-66 | -1.69E-46 |
| MS.gene057392 | MS.gene074606 | 0.800447 | 1.44E-48 | -1.69E-46 |
| MS.gene057444 | MS.gene074606 | 0.815662 | 8.24E-52 | -1.69E-46 |
| MS.gene057693 | MS.gene074606 | 0.830071 | 3.61E-55 | -1.69E-46 |
| MS.gene057694 | MS.gene074606 | 0.800312 | 1.53E-48 | -1.69E-46 |
| MS.gene057705 | MS.gene074606 | 0.820715 | 5.92E-53 | -1.69E-46 |
| MS.gene057855 | MS.gene074606 | 0.801981 | 6.97E-49 | -1.69E-46 |
| MS.gene058008 | MS.gene074606 | 0.840188 | 1.01E-57 | -1.69E-46 |
| MS.gene058009 | MS.gene074606 | 0.860367 | 2.17E-63 | -1.69E-46 |
| MS.gene058037 | MS.gene074606 | 0.821762 | 3.40E-53 | -1.69E-46 |
| MS.gene058088 | MS.gene074606 | 0.832954 | 7.04E-56 | -1.69E-46 |
| MS.gene058548 | MS.gene074606 | 0.80764 | 4.58E-50 | -1.69E-46 |
| MS.gene058549 | MS.gene074606 | 0.826086 | 3.29E-54 | -1.69E-46 |
| MS.gene058625 | MS.gene074606 | 0.827453 | 1.55E-54 | -1.69E-46 |
| MS.gene058781 | MS.gene074606 | 0.82348 | 1.35E-53 | -1.69E-46 |
| MS.gene058783 | MS.gene074606 | 0.884724 | 1.50E-71 | -1.69E-46 |
| MS.gene058979 | MS.gene074606 | 0.829905 | 3.96E-55 | -1.69E-46 |
| MS.gene059151 | MS.gene074606 | 0.845421 | 4.11E-59 | -1.69E-46 |
| MS.gene05954 | MS.gene074606 | 0.823622 | 1.25E-53 | -1.69E-46 |
| MS.gene059625 | MS.gene074606 | 0.805267 | 1.45E-49 | -1.69E-46 |
| MS.gene05979 | MS.gene074606 | 0.845378 | 4.22E-59 | -1.69E-46 |
| MS.gene059842 | MS.gene074606 | 0.867309 | 1.51E-65 | -1.69E-46 |
| MS.gene059935 | MS.gene074606 | 0.803616 | 3.20E-49 | -1.69E-46 |
| MS.gene060244 | MS.gene074606 | 0.804625 | 1.97E-49 | -1.69E-46 |
| MS.gene060322 | MS.gene074606 | 0.836436 | 9.37E-57 | -1.69E-46 |
| MS.gene060365 | MS.gene074606 | 0.807274 | 5.48E-50 | -1.69E-46 |
| MS.gene060429 | MS.gene074606 | 0.851406 | 9.06E-61 | -1.69E-46 |
| MS.gene060572 | MS.gene074606 | 0.808006 | 3.83E-50 | -1.69E-46 |
| MS.gene060803 | MS.gene074606 | 0.811094 | 8.31E-51 | -1.69E-46 |
| MS.gene060917 | MS.gene074606 | 0.821431 | 4.05E-53 | -1.69E-46 |
| MS.gene060935 | MS.gene074606 | 0.840157 | 1.03E-57 | -1.69E-46 |
| MS.gene060937 | MS.gene074606 | 0.807867 | 4.10E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074606.t1 | MTR_4g134170 | 100.000 | 59 | 0 | 0 | 69 | 127 | 648 | 706 | 2.60e-36 | 130 |
| MS.gene074606.t1 | MTR_6g005320 | 96.610 | 59 | 2 | 0 | 69 | 127 | 608 | 666 | 1.68e-34 | 125 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074606.t1 | AT2G33730 | 78.947 | 57 | 12 | 0 | 69 | 125 | 675 | 731 | 5.95e-28 | 107 |
| MS.gene074606.t1 | AT1G28180 | 79.167 | 48 | 10 | 0 | 69 | 116 | 317 | 364 | 3.88e-19 | 81.6 |
Find 39 sgRNAs with CRISPR-Local
Find 36 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATAGATCCTGGCTTGAATT+TGG | 0.231521 | 8.4:+572277 | None:intergenic |
| AGATTTGTCTTGAAGGATTT+AGG | 0.252142 | 8.4:-572566 | MS.gene074606:CDS |
| GCTTCATGCCTTGCCAATTC+AGG | 0.302855 | 8.4:+572302 | None:intergenic |
| CAGAGGGAGATTTGTCTTGA+AGG | 0.377339 | 8.4:-572573 | MS.gene074606:CDS |
| CAGTCCTGTTCCACCTGAAT+TGG | 0.392949 | 8.4:-572315 | MS.gene074606:CDS |
| CCTCAAGAAAATTCTCTAAT+GGG | 0.393770 | 8.4:+572217 | None:intergenic |
| CTTGTTGCAACTGATGTTGC+TGG | 0.393795 | 8.4:-572525 | MS.gene074606:CDS |
| TACAACATTCCTGACTCTTC+AGG | 0.394751 | 8.4:-572384 | MS.gene074606:CDS |
| AAATGTACACCCATCGTATT+GGG | 0.397102 | 8.4:-572440 | MS.gene074606:CDS |
| GAAATGTACACCCATCGTAT+TGG | 0.424823 | 8.4:-572441 | MS.gene074606:CDS |
| CTTGCCAATTCAGGTGGAAC+AGG | 0.427377 | 8.4:+572311 | None:intergenic |
| AACAAGCACATTATATCTCT+TGG | 0.438220 | 8.4:+572542 | None:intergenic |
| GTTATCAACTATGATATGCC+TGG | 0.451885 | 8.4:-572471 | MS.gene074606:CDS |
| TTGATAACATGAGCCACATC+AGG | 0.497781 | 8.4:+572486 | None:intergenic |
| ACCCATCGTATTGGGCGTAC+TGG | 0.502956 | 8.4:-572432 | MS.gene074606:CDS |
| CGTGCAGGAAAGACGGGTCG+TGG | 0.504710 | 8.4:-572408 | MS.gene074606:CDS |
| CATCAGTGTCCTGAAGAGTC+AGG | 0.517770 | 8.4:+572375 | None:intergenic |
| ATTTCGCCTAGGTGGTCTGT+CGG | 0.525141 | 8.4:+572253 | None:intergenic |
| TACTGGTCGTGCAGGAAAGA+CGG | 0.526337 | 8.4:-572415 | MS.gene074606:CDS |
| GCGTGACCACTTTGCATGGA+GGG | 0.527382 | 8.4:-572608 | None:intergenic |
| ACTGGTCGTGCAGGAAAGAC+GGG | 0.527412 | 8.4:-572414 | MS.gene074606:CDS |
| GGTCTGTCGGGAATAGATCC+TGG | 0.531479 | 8.4:+572266 | None:intergenic |
| TTTCGCCTAGGTGGTCTGTC+GGG | 0.533463 | 8.4:+572254 | None:intergenic |
| GCAACTGATGTTGCTGGACG+TGG | 0.534104 | 8.4:-572519 | MS.gene074606:CDS |
| ATTGGGCGTACTGGTCGTGC+AGG | 0.537891 | 8.4:-572423 | MS.gene074606:CDS |
| GTGTACATTTCAATATTCCC+AGG | 0.556474 | 8.4:+572453 | None:intergenic |
| TTATCAACTATGATATGCCT+GGG | 0.566309 | 8.4:-572470 | MS.gene074606:CDS |
| ACCAGTACGCCCAATACGAT+GGG | 0.574501 | 8.4:+572431 | None:intergenic |
| TCATGCCTTGCCAATTCAGG+TGG | 0.578546 | 8.4:+572305 | None:intergenic |
| GAAGCTTCCAAATTCAAGCC+AGG | 0.584125 | 8.4:-572284 | MS.gene074606:CDS |
| GAGGGAAATCACAGGATCAG+AGG | 0.584484 | 8.4:-572590 | MS.gene074606:CDS |
| GACCAGTACGCCCAATACGA+TGG | 0.591341 | 8.4:+572430 | None:intergenic |
| CTGTTCCACCTGAATTGGCA+AGG | 0.607946 | 8.4:-572310 | MS.gene074606:CDS |
| AGGGAAATCACAGGATCAGA+GGG | 0.612491 | 8.4:-572589 | MS.gene074606:CDS |
| AAAACAGTGTCATTTCGCCT+AGG | 0.628001 | 8.4:+572242 | None:intergenic |
| TTTGCATGGAGGGAAATCAC+AGG | 0.635122 | 8.4:-572598 | None:intergenic |
| ACAGTGTCATTTCGCCTAGG+TGG | 0.641491 | 8.4:+572245 | None:intergenic |
| TGGTATTGACATTCCTGATG+TGG | 0.647427 | 8.4:-572499 | MS.gene074606:CDS |
| CTATTCCCGACAGACCACCT+AGG | 0.700511 | 8.4:-572259 | MS.gene074606:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AACAAGCACATTATATCTCT+TGG | + | chr8.4:572286-572305 | None:intergenic | 30.0% | |
| AGATTTGTCTTGAAGGATTT+AGG | - | chr8.4:572259-572278 | MS.gene074606:CDS | 30.0% | |
| TTATCAACTATGATATGCCT+GGG | - | chr8.4:572355-572374 | MS.gene074606:CDS | 30.0% | |
| AAATGTACACCCATCGTATT+GGG | - | chr8.4:572385-572404 | MS.gene074606:CDS | 35.0% | |
| GTGTACATTTCAATATTCCC+AGG | + | chr8.4:572375-572394 | None:intergenic | 35.0% | |
| GTTATCAACTATGATATGCC+TGG | - | chr8.4:572354-572373 | MS.gene074606:CDS | 35.0% | |
| ! | AATAGATCCTGGCTTGAATT+TGG | + | chr8.4:572551-572570 | None:intergenic | 35.0% |
| GAAATGTACACCCATCGTAT+TGG | - | chr8.4:572384-572403 | MS.gene074606:CDS | 40.0% | |
| TACAACATTCCTGACTCTTC+AGG | - | chr8.4:572441-572460 | MS.gene074606:CDS | 40.0% | |
| ! | TTGATAACATGAGCCACATC+AGG | + | chr8.4:572342-572361 | None:intergenic | 40.0% |
| !! | AAAACAGTGTCATTTCGCCT+AGG | + | chr8.4:572586-572605 | None:intergenic | 40.0% |
| !! | TGGTATTGACATTCCTGATG+TGG | - | chr8.4:572326-572345 | MS.gene074606:CDS | 40.0% |
| AGGGAAATCACAGGATCAGA+GGG | - | chr8.4:572236-572255 | MS.gene074606:CDS | 45.0% | |
| CAGAGGGAGATTTGTCTTGA+AGG | - | chr8.4:572252-572271 | MS.gene074606:CDS | 45.0% | |
| CTTGTTGCAACTGATGTTGC+TGG | - | chr8.4:572300-572319 | MS.gene074606:CDS | 45.0% | |
| GAAGCTTCCAAATTCAAGCC+AGG | - | chr8.4:572541-572560 | MS.gene074606:CDS | 45.0% | |
| ACCAGTACGCCCAATACGAT+GGG | + | chr8.4:572397-572416 | None:intergenic | 50.0% | |
| ATTTCGCCTAGGTGGTCTGT+CGG | + | chr8.4:572575-572594 | None:intergenic | 50.0% | |
| CAGTCCTGTTCCACCTGAAT+TGG | - | chr8.4:572510-572529 | MS.gene074606:CDS | 50.0% | |
| CATCAGTGTCCTGAAGAGTC+AGG | + | chr8.4:572453-572472 | None:intergenic | 50.0% | |
| CTGTTCCACCTGAATTGGCA+AGG | - | chr8.4:572515-572534 | MS.gene074606:CDS | 50.0% | |
| CTTGCCAATTCAGGTGGAAC+AGG | + | chr8.4:572517-572536 | None:intergenic | 50.0% | |
| GAGGGAAATCACAGGATCAG+AGG | - | chr8.4:572235-572254 | MS.gene074606:CDS | 50.0% | |
| GCTTCATGCCTTGCCAATTC+AGG | + | chr8.4:572526-572545 | None:intergenic | 50.0% | |
| TACTGGTCGTGCAGGAAAGA+CGG | - | chr8.4:572410-572429 | MS.gene074606:CDS | 50.0% | |
| TCATGCCTTGCCAATTCAGG+TGG | + | chr8.4:572523-572542 | None:intergenic | 50.0% | |
| !! | ACAGTGTCATTTCGCCTAGG+TGG | + | chr8.4:572583-572602 | None:intergenic | 50.0% |
| ACCCATCGTATTGGGCGTAC+TGG | - | chr8.4:572393-572412 | MS.gene074606:CDS | 55.0% | |
| ACTGGTCGTGCAGGAAAGAC+GGG | - | chr8.4:572411-572430 | MS.gene074606:CDS | 55.0% | |
| CTATTCCCGACAGACCACCT+AGG | - | chr8.4:572566-572585 | MS.gene074606:CDS | 55.0% | |
| GACCAGTACGCCCAATACGA+TGG | + | chr8.4:572398-572417 | None:intergenic | 55.0% | |
| GCAACTGATGTTGCTGGACG+TGG | - | chr8.4:572306-572325 | MS.gene074606:CDS | 55.0% | |
| GGTCTGTCGGGAATAGATCC+TGG | + | chr8.4:572562-572581 | None:intergenic | 55.0% | |
| TTTCGCCTAGGTGGTCTGTC+GGG | + | chr8.4:572574-572593 | None:intergenic | 55.0% | |
| ! | ATTGGGCGTACTGGTCGTGC+AGG | - | chr8.4:572402-572421 | MS.gene074606:CDS | 60.0% |
| CGTGCAGGAAAGACGGGTCG+TGG | - | chr8.4:572417-572436 | MS.gene074606:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 572232 | 572615 | 572232 | ID=MS.gene074606 |
| chr8.4 | mRNA | 572232 | 572615 | 572232 | ID=MS.gene074606.t1;Parent=MS.gene074606 |
| chr8.4 | exon | 572232 | 572615 | 572232 | ID=MS.gene074606.t1.exon1;Parent=MS.gene074606.t1 |
| chr8.4 | CDS | 572232 | 572615 | 572232 | ID=cds.MS.gene074606.t1;Parent=MS.gene074606.t1 |
| Gene Sequence |
| Protein sequence |
