AlfalfaGEDB | MS.gene99848

M. sativa cultivar XinJiangDaYe / MS.gene99848

KEGG: -
GO: -
PFAM: PF12776.10

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene99848.t1	KEH33904.1	91.1	135	12	0	1	135	143	277	5.70E-64	253.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene99848.t1	A0A072UVS3	91.1	135	12	0	1	135	143	277	4.1e-64	253.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049254	MS.gene99848	0.852621	4.09E-61	-1.69E-46
MS.gene049293	MS.gene99848	-0.829748	4.33E-55	-1.69E-46
MS.gene049343	MS.gene99848	0.80298	4.34E-49	-1.69E-46
MS.gene049404	MS.gene99848	0.809632	1.72E-50	-1.69E-46
MS.gene049669	MS.gene99848	0.827238	1.75E-54	-1.69E-46
MS.gene04976	MS.gene99848	0.839015	2.04E-57	-1.69E-46
MS.gene049825	MS.gene99848	0.812856	3.43E-51	-1.69E-46
MS.gene050026	MS.gene99848	0.813035	3.14E-51	-1.69E-46
MS.gene05016	MS.gene99848	0.845339	4.32E-59	-1.69E-46
MS.gene050201	MS.gene99848	0.817731	2.83E-52	-1.69E-46
MS.gene050228	MS.gene99848	0.809624	1.73E-50	-1.69E-46
MS.gene050253	MS.gene99848	0.802347	5.86E-49	-1.69E-46
MS.gene050442	MS.gene99848	0.801896	7.25E-49	-1.69E-46
MS.gene050559	MS.gene99848	0.831223	1.89E-55	-1.69E-46
MS.gene050561	MS.gene99848	0.829823	4.15E-55	-1.69E-46
MS.gene050562	MS.gene99848	0.818745	1.67E-52	-1.69E-46
MS.gene050780	MS.gene99848	0.851003	1.18E-60	-1.69E-46
MS.gene050807	MS.gene99848	0.816798	4.59E-52	-1.69E-46
MS.gene051247	MS.gene99848	0.830392	3.01E-55	-1.69E-46
MS.gene051279	MS.gene99848	0.819053	1.42E-52	-1.69E-46
MS.gene051332	MS.gene99848	0.812992	3.21E-51	-1.69E-46
MS.gene051492	MS.gene99848	0.845428	4.09E-59	-1.69E-46
MS.gene051594	MS.gene99848	0.820388	7.04E-53	-1.69E-46
MS.gene051676	MS.gene99848	0.820391	7.03E-53	-1.69E-46
MS.gene051744	MS.gene99848	0.844309	8.19E-59	-1.69E-46
MS.gene051935	MS.gene99848	0.808727	2.69E-50	-1.69E-46
MS.gene051957	MS.gene99848	0.809334	1.99E-50	-1.69E-46
MS.gene052108	MS.gene99848	0.835835	1.33E-56	-1.69E-46
MS.gene052176	MS.gene99848	0.812232	4.70E-51	-1.69E-46
MS.gene052190	MS.gene99848	0.801628	8.23E-49	-1.69E-46
MS.gene052208	MS.gene99848	0.822201	2.69E-53	-1.69E-46
MS.gene052239	MS.gene99848	-0.823729	1.18E-53	-1.69E-46
MS.gene052333	MS.gene99848	0.80609	9.74E-50	-1.69E-46
MS.gene052358	MS.gene99848	-0.809283	2.04E-50	-1.69E-46
MS.gene052519	MS.gene99848	0.816473	5.43E-52	-1.69E-46
MS.gene052534	MS.gene99848	0.803166	3.97E-49	-1.69E-46
MS.gene052582	MS.gene99848	0.822799	1.95E-53	-1.69E-46
MS.gene052584	MS.gene99848	0.827632	1.41E-54	-1.69E-46
MS.gene052586	MS.gene99848	0.841283	5.22E-58	-1.69E-46
MS.gene052768	MS.gene99848	0.802022	6.83E-49	-1.69E-46
MS.gene052773	MS.gene99848	0.829496	4.98E-55	-1.69E-46
MS.gene053006	MS.gene99848	0.826026	3.40E-54	-1.69E-46
MS.gene053045	MS.gene99848	0.816191	6.28E-52	-1.69E-46
MS.gene053054	MS.gene99848	-0.801017	1.10E-48	-1.69E-46
MS.gene053094	MS.gene99848	0.805534	1.27E-49	-1.69E-46
MS.gene053166	MS.gene99848	0.82352	1.33E-53	-1.69E-46
MS.gene053189	MS.gene99848	0.800426	1.45E-48	-1.69E-46
MS.gene053226	MS.gene99848	0.835324	1.79E-56	-1.69E-46
MS.gene053340	MS.gene99848	0.827275	1.71E-54	-1.69E-46
MS.gene053563	MS.gene99848	0.822801	1.95E-53	-1.69E-46
MS.gene053641	MS.gene99848	0.807749	4.34E-50	-1.69E-46
MS.gene05364	MS.gene99848	0.802976	4.34E-49	-1.69E-46
MS.gene053995	MS.gene99848	0.820786	5.71E-53	-1.69E-46
MS.gene05410	MS.gene99848	0.822361	2.47E-53	-1.69E-46
MS.gene05414	MS.gene99848	0.829389	5.29E-55	-1.69E-46
MS.gene05415	MS.gene99848	0.875974	2.02E-68	-1.69E-46
MS.gene054160	MS.gene99848	0.803307	3.71E-49	-1.69E-46
MS.gene054222	MS.gene99848	0.809531	1.81E-50	-1.69E-46
MS.gene05423	MS.gene99848	0.826628	2.44E-54	-1.69E-46
MS.gene054291	MS.gene99848	-0.825656	4.16E-54	-1.69E-46
MS.gene054468	MS.gene99848	0.824155	9.41E-54	-1.69E-46
MS.gene054479	MS.gene99848	0.801872	7.34E-49	-1.69E-46
MS.gene054576	MS.gene99848	0.847753	9.47E-60	-1.69E-46
MS.gene054651	MS.gene99848	0.821248	4.47E-53	-1.69E-46
MS.gene054657	MS.gene99848	0.822397	2.42E-53	-1.69E-46
MS.gene055166	MS.gene99848	0.800926	1.15E-48	-1.69E-46
MS.gene055228	MS.gene99848	0.809956	1.46E-50	-1.69E-46
MS.gene055230	MS.gene99848	0.835056	2.10E-56	-1.69E-46
MS.gene05548	MS.gene99848	0.807831	4.17E-50	-1.69E-46
MS.gene055586	MS.gene99848	0.829342	5.43E-55	-1.69E-46
MS.gene055589	MS.gene99848	0.803796	2.94E-49	-1.69E-46
MS.gene055701	MS.gene99848	0.808598	2.86E-50	-1.69E-46
MS.gene055778	MS.gene99848	0.834629	2.68E-56	-1.69E-46
MS.gene055818	MS.gene99848	0.8438	1.12E-58	-1.69E-46
MS.gene055841	MS.gene99848	0.829108	6.19E-55	-1.69E-46
MS.gene05604	MS.gene99848	0.836601	8.51E-57	-1.69E-46
MS.gene056050	MS.gene99848	0.801798	7.60E-49	-1.69E-46
MS.gene056162	MS.gene99848	0.813679	2.27E-51	-1.69E-46
MS.gene056173	MS.gene99848	0.802931	4.44E-49	-1.69E-46
MS.gene056366	MS.gene99848	0.844289	8.29E-59	-1.69E-46
MS.gene056373	MS.gene99848	0.806501	7.98E-50	-1.69E-46
MS.gene056498	MS.gene99848	0.802971	4.35E-49	-1.69E-46
MS.gene056506	MS.gene99848	0.825007	5.93E-54	-1.69E-46
MS.gene05659	MS.gene99848	0.817651	2.95E-52	-1.69E-46
MS.gene056776	MS.gene99848	0.80965	1.70E-50	-1.69E-46
MS.gene056779	MS.gene99848	0.802028	6.81E-49	-1.69E-46
MS.gene05690	MS.gene99848	0.800258	1.57E-48	-1.69E-46
MS.gene057012	MS.gene99848	0.802875	4.56E-49	-1.69E-46
MS.gene057021	MS.gene99848	0.800327	1.52E-48	-1.69E-46
MS.gene057026	MS.gene99848	0.856782	2.55E-62	-1.69E-46
MS.gene057060	MS.gene99848	0.848906	4.55E-60	-1.69E-46
MS.gene057102	MS.gene99848	0.801425	9.06E-49	-1.69E-46
MS.gene057208	MS.gene99848	0.846172	2.57E-59	-1.69E-46
MS.gene057414	MS.gene99848	0.800762	1.24E-48	-1.69E-46
MS.gene057444	MS.gene99848	0.805301	1.43E-49	-1.69E-46
MS.gene057598	MS.gene99848	0.811581	6.52E-51	-1.69E-46
MS.gene057693	MS.gene99848	0.817045	4.04E-52	-1.69E-46
MS.gene057869	MS.gene99848	0.802217	6.23E-49	-1.69E-46
MS.gene05795	MS.gene99848	0.804182	2.44E-49	-1.69E-46
MS.gene058008	MS.gene99848	0.804162	2.47E-49	-1.69E-46
MS.gene058037	MS.gene99848	0.807049	6.11E-50	-1.69E-46
MS.gene058477	MS.gene99848	0.801913	7.19E-49	-1.69E-46
MS.gene058625	MS.gene99848	0.828911	6.91E-55	-1.69E-46
MS.gene058636	MS.gene99848	0.828271	9.86E-55	-1.69E-46
MS.gene058829	MS.gene99848	0.808314	3.29E-50	-1.69E-46
MS.gene058979	MS.gene99848	0.823285	1.50E-53	-1.69E-46
MS.gene059439	MS.gene99848	0.827702	1.35E-54	-1.69E-46
MS.gene05954	MS.gene99848	0.806276	8.90E-50	-1.69E-46
MS.gene059584	MS.gene99848	0.802531	5.37E-49	-1.69E-46
MS.gene059625	MS.gene99848	0.80734	5.30E-50	-1.69E-46
MS.gene060365	MS.gene99848	0.848999	4.28E-60	-1.69E-46
MS.gene060373	MS.gene99848	0.825815	3.82E-54	-1.69E-46
MS.gene060521	MS.gene99848	0.830098	3.56E-55	-1.69E-46
MS.gene060572	MS.gene99848	0.846119	2.65E-59	-1.69E-46
MS.gene060634	MS.gene99848	-0.82992	3.93E-55	-1.69E-46
MS.gene060879	MS.gene99848	0.825947	3.55E-54	-1.69E-46
MS.gene061040	MS.gene99848	-0.825182	5.39E-54	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene99848.t1	MTR_3g053640	91.111	135	12	0	1	135	143	277	1.79e-86	258
MS.gene99848.t1	MTR_1g094760	60.800	125	37	3	18	135	424	543	1.71e-41	145
MS.gene99848.t1	MTR_4g092300	54.478	134	59	1	4	135	518	651	1.21e-40	144
MS.gene99848.t1	MTR_4g051480	54.478	134	59	1	4	135	536	669	1.36e-40	144
MS.gene99848.t1	MTR_8g085310	58.772	114	37	2	27	135	421	529	5.98e-38	135
MS.gene99848.t1	MTR_3g065345	56.944	72	31	0	64	135	4	75	1.48e-24	90.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 21 sgRNAs with CRISPR-Local

Find 28 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGCAGCAACGGTGTTGGTTT+TGG	0.285560	3.1:-39429397	None:intergenic
CATTTGACATATTTCCATAT+CGG	0.330242	3.1:-39429227	None:intergenic
TGAGCTACTGTTATCATCAT+TGG	0.337549	3.1:-39429133	None:intergenic
GCATTACTTACATCCCGATA+TGG	0.365810	3.1:+39429213	MS.gene99848:CDS
ATTTGACATATTTCCATATC+GGG	0.391955	3.1:-39429226	None:intergenic
TGCAAAACACTGAATTTAAG+TGG	0.399549	3.1:+39429370	MS.gene99848:CDS
AAAGCTTTAGTTTCTAAGTC+AGG	0.404846	3.1:-39429165	None:intergenic
TTGCTGCAACTGCTGAGGTC+TGG	0.441093	3.1:+39429412	MS.gene99848:CDS
TTTATAGGAACGATTTGTTA+CGG	0.462981	3.1:-39429283	None:intergenic
CGAATAAAATTCATGATACA+TGG	0.491448	3.1:-39429343	None:intergenic
GGTCTGGCAAAATTATCTTC+AGG	0.500177	3.1:+39429428	MS.gene99848:CDS
ATTCAACTTTGTAGTCATTG+AGG	0.505655	3.1:-39429259	None:intergenic
GACCTCAGCAGTTGCAGCAA+CGG	0.510351	3.1:-39429409	None:intergenic
AGCAGTTGCAGCAACGGTGT+TGG	0.521094	3.1:-39429403	None:intergenic
GTAGCTGCATATGCGGTATG+AGG	0.531391	3.1:+39428800	None:intergenic
CAAACTGAAGACAAAGTGTC+AGG	0.538802	3.1:+39428863	MS.gene99848:CDS
GATGTGCGCAATCTGATCAT+AGG	0.546455	3.1:-39428841	None:intergenic
ATAACAGTAGCTCAAAGCTA+TGG	0.575966	3.1:+39429142	MS.gene99848:CDS
CACCGTTGCTGCAACTGCTG+AGG	0.597496	3.1:+39429407	MS.gene99848:CDS
GAAATATGTCAAATGACGTG+TGG	0.650090	3.1:+39429235	MS.gene99848:CDS
AACTAAAGGAGAAAATACAT+AGG	0.675289	3.1:+39429313	MS.gene99848:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATAACATAACAATAGAATAT+AGG	-	chr3.1:39428988-39429007	None:intergenic	15.0%
!!	CTATAAAAAAGAACAACTAA+AGG	+	chr3.1:39429299-39429318	MS.gene99848:CDS	20.0%
!!!	CTTTAGTTGTTCTTTTTTAT+AGG	-	chr3.1:39429301-39429320	None:intergenic	20.0%
!	AACTAAAGGAGAAAATACAT+AGG	+	chr3.1:39429313-39429332	MS.gene99848:CDS	25.0%
!	ATAAAGAATGATGATAAGAG+AGG	-	chr3.1:39428914-39428933	None:intergenic	25.0%
!	ATTTGACATATTTCCATATC+GGG	-	chr3.1:39429229-39429248	None:intergenic	25.0%
!	CATTTGACATATTTCCATAT+CGG	-	chr3.1:39429230-39429249	None:intergenic	25.0%
!	CGAATAAAATTCATGATACA+TGG	-	chr3.1:39429346-39429365	None:intergenic	25.0%
!	TAAAGAATGATGATAAGAGA+GGG	-	chr3.1:39428913-39428932	None:intergenic	25.0%
!!!	TTTATAGGAACGATTTGTTA+CGG	-	chr3.1:39429286-39429305	None:intergenic	25.0%
	AACAATAGAATATAGGCTAC+AGG	-	chr3.1:39428981-39429000	None:intergenic	30.0%
	ATTCAACTTTGTAGTCATTG+AGG	-	chr3.1:39429262-39429281	None:intergenic	30.0%
	TGCAAAACACTGAATTTAAG+TGG	+	chr3.1:39429370-39429389	MS.gene99848:CDS	30.0%
!	AAAGCTTTAGTTTCTAAGTC+AGG	-	chr3.1:39429168-39429187	None:intergenic	30.0%
	ATAACAGTAGCTCAAAGCTA+TGG	+	chr3.1:39429142-39429161	MS.gene99848:CDS	35.0%
	GAAATATGTCAAATGACGTG+TGG	+	chr3.1:39429235-39429254	MS.gene99848:CDS	35.0%
	TGAGCTACTGTTATCATCAT+TGG	-	chr3.1:39429136-39429155	None:intergenic	35.0%
	TTTAACCAGCAAAGCAACAA+TGG	-	chr3.1:39428958-39428977	None:intergenic	35.0%
	GCATTACTTACATCCCGATA+TGG	+	chr3.1:39429213-39429232	MS.gene99848:CDS	40.0%
!	CAAACTGAAGACAAAGTGTC+AGG	+	chr3.1:39428863-39428882	MS.gene99848:CDS	40.0%
!	TGAAGACAAAGTGTCAGGTT+TGG	+	chr3.1:39428868-39428887	MS.gene99848:intron	40.0%
!!!	GTTTTCCATTGTTGCTTTGC+TGG	+	chr3.1:39428950-39428969	MS.gene99848:intron	40.0%
	GATGTGCGCAATCTGATCAT+AGG	-	chr3.1:39428844-39428863	None:intergenic	45.0%
!!!	TGCAGCAACGGTGTTGGTTT+TGG	-	chr3.1:39429400-39429419	None:intergenic	50.0%
	AGCAGTTGCAGCAACGGTGT+TGG	-	chr3.1:39429406-39429425	None:intergenic	55.0%
	GACCTCAGCAGTTGCAGCAA+CGG	-	chr3.1:39429412-39429431	None:intergenic	55.0%
	TTGCTGCAACTGCTGAGGTC+TGG	+	chr3.1:39429412-39429431	MS.gene99848:CDS	55.0%
	CACCGTTGCTGCAACTGCTG+AGG	+	chr3.1:39429407-39429426	MS.gene99848:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.1	gene	39428804	39429449	39428804	ID=MS.gene99848
chr3.1	mRNA	39428804	39429449	39428804	ID=MS.gene99848.t1;Parent=MS.gene99848
chr3.1	exon	39428804	39428884	39428804	ID=MS.gene99848.t1.exon1;Parent=MS.gene99848.t1
chr3.1	CDS	39428804	39428884	39428804	ID=cds.MS.gene99848.t1;Parent=MS.gene99848.t1
chr3.1	exon	39429126	39429449	39429126	ID=MS.gene99848.t1.exon2;Parent=MS.gene99848.t1
chr3.1	CDS	39429126	39429449	39429126	ID=cds.MS.gene99848.t1;Parent=MS.gene99848.t1

Gene Sequence

>MS.gene99848

CTGCATATGCGGTATGAGGATGAAATATATGTTGTTTCCTATGATCAGATTGCGCACATCAAACTGAAGACAAAGTGTCAGGTTTGGTCGTAACTGACTTGTTCTTCCCTCTCTTATCATCATTCTTTATACTTGTGCTGCTATAAGTTTTCCATTGTTGCTTTGCTGGTTAAACCTGTAGCCTATATTCTATTGTTATGTTATATATGCATTAAGTAGTAATAGTAATAGTAATAGTAATACTAATACTAATACTAATAATAATAACAAAGTCTAATTGCTTCAAAAAAAAACAAAGTCTAATTGCTTATTTTTATGATAGATGACAACCAATGATGATAACAGTAGCTCAAAGCTATGGCCTGACTTAGAAACTAAAGCTTTCATAAATATTATGCTTGATGAAGTTGCATTACTTACATCCCGATATGGAAATATGTCAAATGACGTGTGGACCTCAATGACTACAAAGTTGAATGCCGTAACAAATCGTTCCTATAAAAAAGAACAACTAAAGGAGAAAATACATAGGCTACATGCCATGTATCATGAATTTTATTCGCTCTTGCAAAACACTGAATTTAAGTGGAATGCCAAAACCAACACCGTTGCTGCAACTGCTGAGGTCTGGCAAAATTATCTTCAG

Protein sequence

>MS.gene99848.t1

LHMRYEDEIYVVSYDQIAHIKLKTKCQMTTNDDNSSSKLWPDLETKAFINIMLDEVALLTSRYGNMSNDVWTSMTTKLNAVTNRSYKKEQLKEKIHRLHAMYHEFYSLLQNTEFKWNAKTNTVAATAEVWQNYLQ