Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91676.t1 | KEH30621.1 | 95.2 | 63 | 3 | 0 | 6 | 68 | 307 | 369 | 2.50E-23 | 117.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91676.t1 | Q84P54 | 82.3 | 62 | 11 | 0 | 6 | 67 | 454 | 515 | 3.6e-23 | 108.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91676.t1 | A0A072UMK4 | 95.2 | 63 | 3 | 0 | 6 | 68 | 307 | 369 | 1.8e-23 | 117.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049159 | MS.gene91676 | 0.816579 | 5.14E-52 | -1.69E-46 |
| MS.gene049254 | MS.gene91676 | 0.849631 | 2.86E-60 | -1.69E-46 |
| MS.gene049404 | MS.gene91676 | 0.831093 | 2.03E-55 | -1.69E-46 |
| MS.gene049873 | MS.gene91676 | 0.804923 | 1.71E-49 | -1.69E-46 |
| MS.gene050026 | MS.gene91676 | 0.82257 | 2.21E-53 | -1.69E-46 |
| MS.gene050355 | MS.gene91676 | 0.809547 | 1.79E-50 | -1.69E-46 |
| MS.gene050442 | MS.gene91676 | 0.81453 | 1.47E-51 | -1.69E-46 |
| MS.gene050559 | MS.gene91676 | 0.809121 | 2.21E-50 | -1.69E-46 |
| MS.gene050561 | MS.gene91676 | 0.809607 | 1.74E-50 | -1.69E-46 |
| MS.gene050680 | MS.gene91676 | 0.818187 | 2.23E-52 | -1.69E-46 |
| MS.gene050750 | MS.gene91676 | 0.837795 | 4.21E-57 | -1.69E-46 |
| MS.gene050844 | MS.gene91676 | 0.814739 | 1.32E-51 | -1.69E-46 |
| MS.gene050953 | MS.gene91676 | 0.826334 | 2.87E-54 | -1.69E-46 |
| MS.gene051181 | MS.gene91676 | 0.845987 | 2.88E-59 | -1.69E-46 |
| MS.gene051247 | MS.gene91676 | 0.813925 | 2.00E-51 | -1.69E-46 |
| MS.gene051265 | MS.gene91676 | 0.826099 | 3.27E-54 | -1.69E-46 |
| MS.gene051279 | MS.gene91676 | 0.803182 | 3.94E-49 | -1.69E-46 |
| MS.gene051574 | MS.gene91676 | 0.800039 | 1.74E-48 | -1.69E-46 |
| MS.gene051594 | MS.gene91676 | 0.84142 | 4.80E-58 | -1.69E-46 |
| MS.gene051744 | MS.gene91676 | 0.830921 | 2.24E-55 | -1.69E-46 |
| MS.gene051957 | MS.gene91676 | 0.81357 | 2.39E-51 | -1.69E-46 |
| MS.gene052190 | MS.gene91676 | 0.83508 | 2.07E-56 | -1.69E-46 |
| MS.gene052208 | MS.gene91676 | 0.819964 | 8.81E-53 | -1.69E-46 |
| MS.gene052333 | MS.gene91676 | 0.822906 | 1.84E-53 | -1.69E-46 |
| MS.gene052488 | MS.gene91676 | 0.80196 | 7.04E-49 | -1.69E-46 |
| MS.gene052519 | MS.gene91676 | 0.862663 | 4.32E-64 | -1.69E-46 |
| MS.gene052582 | MS.gene91676 | 0.826186 | 3.11E-54 | -1.69E-46 |
| MS.gene052584 | MS.gene91676 | 0.822752 | 2.00E-53 | -1.69E-46 |
| MS.gene052611 | MS.gene91676 | 0.807986 | 3.87E-50 | -1.69E-46 |
| MS.gene052773 | MS.gene91676 | 0.815751 | 7.87E-52 | -1.69E-46 |
| MS.gene052863 | MS.gene91676 | 0.823708 | 1.20E-53 | -1.69E-46 |
| MS.gene052867 | MS.gene91676 | 0.840618 | 7.80E-58 | -1.69E-46 |
| MS.gene053006 | MS.gene91676 | 0.817967 | 2.51E-52 | -1.69E-46 |
| MS.gene053145 | MS.gene91676 | 0.829242 | 5.74E-55 | -1.69E-46 |
| MS.gene053154 | MS.gene91676 | 0.800211 | 1.60E-48 | -1.69E-46 |
| MS.gene053166 | MS.gene91676 | 0.849991 | 2.27E-60 | -1.69E-46 |
| MS.gene053201 | MS.gene91676 | 0.803669 | 3.12E-49 | -1.69E-46 |
| MS.gene053226 | MS.gene91676 | 0.833304 | 5.76E-56 | -1.69E-46 |
| MS.gene05365 | MS.gene91676 | 0.805304 | 1.42E-49 | -1.69E-46 |
| MS.gene053883 | MS.gene91676 | 0.813423 | 2.58E-51 | -1.69E-46 |
| MS.gene053900 | MS.gene91676 | 0.807968 | 3.90E-50 | -1.69E-46 |
| MS.gene053998 | MS.gene91676 | 0.834661 | 2.63E-56 | -1.69E-46 |
| MS.gene05414 | MS.gene91676 | 0.820968 | 5.18E-53 | -1.69E-46 |
| MS.gene05415 | MS.gene91676 | 0.851019 | 1.17E-60 | -1.69E-46 |
| MS.gene054160 | MS.gene91676 | 0.811638 | 6.34E-51 | -1.69E-46 |
| MS.gene054236 | MS.gene91676 | 0.824578 | 7.48E-54 | -1.69E-46 |
| MS.gene054291 | MS.gene91676 | -0.816397 | 5.65E-52 | -1.69E-46 |
| MS.gene054405 | MS.gene91676 | 0.82589 | 3.66E-54 | -1.69E-46 |
| MS.gene054467 | MS.gene91676 | 0.816911 | 4.33E-52 | -1.69E-46 |
| MS.gene054559 | MS.gene91676 | 0.84455 | 7.05E-59 | -1.69E-46 |
| MS.gene054651 | MS.gene91676 | 0.853529 | 2.25E-61 | -1.69E-46 |
| MS.gene054775 | MS.gene91676 | 0.826819 | 2.20E-54 | -1.69E-46 |
| MS.gene055227 | MS.gene91676 | 0.833736 | 4.49E-56 | -1.69E-46 |
| MS.gene055228 | MS.gene91676 | 0.854892 | 9.09E-62 | -1.69E-46 |
| MS.gene055356 | MS.gene91676 | 0.807193 | 5.70E-50 | -1.69E-46 |
| MS.gene055357 | MS.gene91676 | 0.832767 | 7.83E-56 | -1.69E-46 |
| MS.gene055586 | MS.gene91676 | 0.808351 | 3.23E-50 | -1.69E-46 |
| MS.gene055589 | MS.gene91676 | 0.82272 | 2.04E-53 | -1.69E-46 |
| MS.gene055778 | MS.gene91676 | 0.81264 | 3.83E-51 | -1.69E-46 |
| MS.gene055818 | MS.gene91676 | 0.865368 | 6.21E-65 | -1.69E-46 |
| MS.gene05604 | MS.gene91676 | 0.806251 | 9.01E-50 | -1.69E-46 |
| MS.gene056162 | MS.gene91676 | 0.807695 | 4.46E-50 | -1.69E-46 |
| MS.gene056174 | MS.gene91676 | 0.816343 | 5.81E-52 | -1.69E-46 |
| MS.gene056252 | MS.gene91676 | 0.803245 | 3.82E-49 | -1.69E-46 |
| MS.gene056366 | MS.gene91676 | 0.85383 | 1.84E-61 | -1.69E-46 |
| MS.gene056373 | MS.gene91676 | 0.803486 | 3.41E-49 | -1.69E-46 |
| MS.gene056498 | MS.gene91676 | 0.818886 | 1.55E-52 | -1.69E-46 |
| MS.gene056506 | MS.gene91676 | 0.832833 | 7.54E-56 | -1.69E-46 |
| MS.gene057021 | MS.gene91676 | 0.816965 | 4.21E-52 | -1.69E-46 |
| MS.gene057026 | MS.gene91676 | 0.819051 | 1.42E-52 | -1.69E-46 |
| MS.gene057027 | MS.gene91676 | 0.802092 | 6.61E-49 | -1.69E-46 |
| MS.gene057060 | MS.gene91676 | 0.851604 | 7.96E-61 | -1.69E-46 |
| MS.gene057208 | MS.gene91676 | 0.819076 | 1.40E-52 | -1.69E-46 |
| MS.gene057314 | MS.gene91676 | 0.819916 | 9.03E-53 | -1.69E-46 |
| MS.gene057444 | MS.gene91676 | 0.832978 | 6.94E-56 | -1.69E-46 |
| MS.gene057693 | MS.gene91676 | 0.81332 | 2.72E-51 | -1.69E-46 |
| MS.gene058009 | MS.gene91676 | 0.805204 | 1.49E-49 | -1.69E-46 |
| MS.gene058037 | MS.gene91676 | 0.844733 | 6.30E-59 | -1.69E-46 |
| MS.gene058294 | MS.gene91676 | 0.814315 | 1.64E-51 | -1.69E-46 |
| MS.gene058625 | MS.gene91676 | 0.829173 | 5.97E-55 | -1.69E-46 |
| MS.gene058783 | MS.gene91676 | 0.802223 | 6.21E-49 | -1.69E-46 |
| MS.gene058979 | MS.gene91676 | 0.837993 | 3.74E-57 | -1.69E-46 |
| MS.gene059128 | MS.gene91676 | 0.811566 | 6.57E-51 | -1.69E-46 |
| MS.gene059272 | MS.gene91676 | 0.8092 | 2.13E-50 | -1.69E-46 |
| MS.gene059382 | MS.gene91676 | 0.813011 | 3.18E-51 | -1.69E-46 |
| MS.gene05954 | MS.gene91676 | 0.817432 | 3.31E-52 | -1.69E-46 |
| MS.gene059584 | MS.gene91676 | 0.855648 | 5.48E-62 | -1.69E-46 |
| MS.gene059625 | MS.gene91676 | 0.812704 | 3.71E-51 | -1.69E-46 |
| MS.gene060011 | MS.gene91676 | 0.816006 | 6.91E-52 | -1.69E-46 |
| MS.gene060310 | MS.gene91676 | 0.814369 | 1.60E-51 | -1.69E-46 |
| MS.gene060312 | MS.gene91676 | 0.801638 | 8.19E-49 | -1.69E-46 |
| MS.gene060322 | MS.gene91676 | 0.851675 | 7.61E-61 | -1.69E-46 |
| MS.gene060429 | MS.gene91676 | 0.811466 | 6.90E-51 | -1.69E-46 |
| MS.gene060521 | MS.gene91676 | 0.833447 | 5.31E-56 | -1.69E-46 |
| MS.gene060553 | MS.gene91676 | 0.807841 | 4.15E-50 | -1.69E-46 |
| MS.gene060572 | MS.gene91676 | 0.82072 | 5.91E-53 | -1.69E-46 |
| MS.gene061040 | MS.gene91676 | -0.810348 | 1.21E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91676.t1 | MTR_4g076100 | 95.238 | 63 | 3 | 0 | 6 | 68 | 307 | 369 | 3.84e-36 | 124 |
| MS.gene91676.t1 | MTR_4g076100 | 95.238 | 63 | 3 | 0 | 6 | 68 | 451 | 513 | 9.25e-36 | 125 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91676.t1 | AT3G22200 | 74.603 | 63 | 16 | 0 | 6 | 68 | 450 | 512 | 8.55e-29 | 106 |
| MS.gene91676.t1 | AT3G22200 | 74.603 | 63 | 16 | 0 | 6 | 68 | 441 | 503 | 1.33e-28 | 105 |
Find 17 sgRNAs with CRISPR-Local
Find 48 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCCAGTTAATCAGCATTTA+TGG | 0.145879 | 7.4:+86351297 | MS.gene91676:intron |
| ACAGGAGTAGGTGCCTATTT+TGG | 0.230656 | 7.4:+86350533 | MS.gene91676:CDS |
| TCTTCAGGTGATATAATAAA+TGG | 0.277964 | 7.4:-86350620 | None:intergenic |
| GGGATGCTAGTTCGTGTAGC+TGG | 0.294169 | 7.4:+86350575 | MS.gene91676:CDS |
| CTCGCACTGTGCTCCAAAAT+AGG | 0.327909 | 7.4:-86350546 | None:intergenic |
| GTTACCTCGTCAACTTCTTC+AGG | 0.342007 | 7.4:-86350635 | None:intergenic |
| CCCAGTTAATCAGCATTTAT+GGG | 0.347963 | 7.4:+86351298 | MS.gene91676:intron |
| AACACTATGATATACATTAC+AGG | 0.386223 | 7.4:+86350515 | None:intergenic |
| CGGCTACTTGCGTTGAGACT+TGG | 0.389136 | 7.4:-86351358 | None:intergenic |
| GGATGCTAGTTCGTGTAGCT+GGG | 0.441540 | 7.4:+86350576 | MS.gene91676:CDS |
| TGGGAAAGCTTTAAGAGAGA+CGG | 0.523625 | 7.4:+86351317 | MS.gene91676:CDS |
| ATGATATACATTACAGGAGT+AGG | 0.523797 | 7.4:+86350521 | MS.gene91676:CDS |
| GGAGCACAGTGCGAGAAGCG+TGG | 0.580915 | 7.4:+86350554 | MS.gene91676:CDS |
| GAGCACAGTGCGAGAAGCGT+GGG | 0.583956 | 7.4:+86350555 | MS.gene91676:CDS |
| TCAGGTGATATAATAAATGG+TGG | 0.616932 | 7.4:-86350617 | None:intergenic |
| GATGCTAGTTCGTGTAGCTG+GGG | 0.670876 | 7.4:+86350577 | MS.gene91676:CDS |
| ATCACCTGAAGAAGTTGACG+AGG | 0.716638 | 7.4:+86350631 | MS.gene91676:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTAAATTATTGAATATGAC+CGG | + | chr7.4:86350750-86350769 | MS.gene91676:intron | 15.0% |
| !!! | TTTTCTTAAAATTTAACATC+TGG | + | chr7.4:86350705-86350724 | MS.gene91676:intron | 15.0% |
| !! | AATTGTACATACAAAGTAAT+TGG | + | chr7.4:86350928-86350947 | MS.gene91676:intron | 20.0% |
| !! | CTGAATAATAATATAAACGA+AGG | - | chr7.4:86351202-86351221 | None:intergenic | 20.0% |
| !!! | TAAATACTCTTTTATTTGAG+TGG | + | chr7.4:86350893-86350912 | MS.gene91676:intron | 20.0% |
| ! | CAAAAATTCAATAGAACCAT+AGG | - | chr7.4:86351091-86351110 | None:intergenic | 25.0% |
| ! | CTTTCTTCAATTTAATGAAG+TGG | - | chr7.4:86350685-86350704 | None:intergenic | 25.0% |
| ! | TCTTCAGGTGATATAATAAA+TGG | - | chr7.4:86350623-86350642 | None:intergenic | 25.0% |
| !! | GATACTGCAGATTTTAAATT+TGG | + | chr7.4:86351241-86351260 | MS.gene91676:intron | 25.0% |
| !! | GTTGCAAAATTTTCAAATGA+AGG | + | chr7.4:86350858-86350877 | MS.gene91676:intron | 25.0% |
| ACTAACTAGTCAATCACTTT+GGG | - | chr7.4:86351061-86351080 | None:intergenic | 30.0% | |
| ATCGAAACATACAAGTCAAT+CGG | + | chr7.4:86350979-86350998 | MS.gene91676:intron | 30.0% | |
| ATGATATACATTACAGGAGT+AGG | + | chr7.4:86350521-86350540 | MS.gene91676:CDS | 30.0% | |
| TACTAACTAGTCAATCACTT+TGG | - | chr7.4:86351062-86351081 | None:intergenic | 30.0% | |
| TCAGGTGATATAATAAATGG+TGG | - | chr7.4:86350620-86350639 | None:intergenic | 30.0% | |
| ! | TGATTGACTAGTTAGTATGA+TGG | + | chr7.4:86351065-86351084 | MS.gene91676:intron | 30.0% |
| CCCAGTTAATCAGCATTTAT+GGG | + | chr7.4:86351298-86351317 | MS.gene91676:intron | 35.0% | |
| TATGTTATGCTTGTTTGCAG+AGG | - | chr7.4:86351157-86351176 | None:intergenic | 35.0% | |
| TCCCAGTTAATCAGCATTTA+TGG | + | chr7.4:86351297-86351316 | MS.gene91676:intron | 35.0% | |
| ! | CCCATAAATGCTGATTAACT+GGG | - | chr7.4:86351301-86351320 | None:intergenic | 35.0% |
| ! | TCCCATAAATGCTGATTAAC+TGG | - | chr7.4:86351302-86351321 | None:intergenic | 35.0% |
| ! | TGCTGATTAACTGGGAAAAA+AGG | - | chr7.4:86351293-86351312 | None:intergenic | 35.0% |
| CTAGTTAGTATGATGGCCTA+TGG | + | chr7.4:86351072-86351091 | MS.gene91676:intron | 40.0% | |
| CTCTTCTGTTCAATCATCCT+AGG | + | chr7.4:86350787-86350806 | MS.gene91676:intron | 40.0% | |
| GAAACAAGAGTTTCCCTGAT+AGG | - | chr7.4:86351017-86351036 | None:intergenic | 40.0% | |
| GACTTGTATGTTTCGATGTG+TGG | - | chr7.4:86350976-86350995 | None:intergenic | 40.0% | |
| !! | TGGGAAAGCTTTAAGAGAGA+CGG | + | chr7.4:86351317-86351336 | MS.gene91676:CDS | 40.0% |
| AGGTTCTTTAGCGACCTCAT+TGG | + | chr7.4:86350807-86350826 | MS.gene91676:intron | 45.0% | |
| ATCACCTGAAGAAGTTGACG+AGG | + | chr7.4:86350631-86350650 | MS.gene91676:CDS | 45.0% | |
| ATGAGGTCGCTAAAGAACCT+AGG | - | chr7.4:86350807-86350826 | None:intergenic | 45.0% | |
| CAGAGGAAACATGTGACTGT+TGG | - | chr7.4:86351140-86351159 | None:intergenic | 45.0% | |
| GTTACCTCGTCAACTTCTTC+AGG | - | chr7.4:86350638-86350657 | None:intergenic | 45.0% | |
| TCTTTAGCGACCTCATTGGT+GGG | + | chr7.4:86350811-86350830 | MS.gene91676:intron | 45.0% | |
| TTATCATGTCCCCACCAATG+AGG | - | chr7.4:86350824-86350843 | None:intergenic | 45.0% | |
| TTCTTTAGCGACCTCATTGG+TGG | + | chr7.4:86350810-86350829 | MS.gene91676:intron | 45.0% | |
| ! | AAGAGTGTGTTTCTTCACGC+CGG | - | chr7.4:86350772-86350791 | None:intergenic | 45.0% |
| ! | ACAGGAGTAGGTGCCTATTT+TGG | + | chr7.4:86350533-86350552 | MS.gene91676:CDS | 45.0% |
| !! | GAAAAAAAAAAAAAAAAAAT+TGG | + | chr7.4:86350950-86350969 | MS.gene91676:intron | 5.0% |
| CTCGCACTGTGCTCCAAAAT+AGG | - | chr7.4:86350549-86350568 | None:intergenic | 50.0% | |
| CTTTAGCGACCTCATTGGTG+GGG | + | chr7.4:86350812-86350831 | MS.gene91676:intron | 50.0% | |
| GATGCTAGTTCGTGTAGCTG+GGG | + | chr7.4:86350577-86350596 | MS.gene91676:CDS | 50.0% | |
| GGATGCTAGTTCGTGTAGCT+GGG | + | chr7.4:86350576-86350595 | MS.gene91676:CDS | 50.0% | |
| TCCCTGATAGGAATGTGCAG+CGG | - | chr7.4:86351005-86351024 | None:intergenic | 50.0% | |
| GCCGCTGCACATTCCTATCA+GGG | + | chr7.4:86351001-86351020 | MS.gene91676:intron | 55.0% | |
| GGGATGCTAGTTCGTGTAGC+TGG | + | chr7.4:86350575-86350594 | MS.gene91676:CDS | 55.0% | |
| GGCCGCTGCACATTCCTATC+AGG | + | chr7.4:86351000-86351019 | MS.gene91676:intron | 60.0% | |
| ! | GAGCACAGTGCGAGAAGCGT+GGG | + | chr7.4:86350555-86350574 | MS.gene91676:CDS | 60.0% |
| GGAGCACAGTGCGAGAAGCG+TGG | + | chr7.4:86350554-86350573 | MS.gene91676:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 86350521 | 86351377 | 86350521 | ID=MS.gene91676 |
| chr7.4 | mRNA | 86350521 | 86351377 | 86350521 | ID=MS.gene91676.t1;Parent=MS.gene91676 |
| chr7.4 | exon | 86350521 | 86350652 | 86350521 | ID=MS.gene91676.t1.exon1;Parent=MS.gene91676.t1 |
| chr7.4 | CDS | 86350521 | 86350652 | 86350521 | ID=cds.MS.gene91676.t1;Parent=MS.gene91676.t1 |
| chr7.4 | exon | 86351303 | 86351377 | 86351303 | ID=MS.gene91676.t1.exon2;Parent=MS.gene91676.t1 |
| chr7.4 | CDS | 86351303 | 86351377 | 86351303 | ID=cds.MS.gene91676.t1;Parent=MS.gene91676.t1 |
| Gene Sequence |
| Protein sequence |
