Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068385.t1 | KEH39608.1 | 97.8 | 270 | 6 | 0 | 1 | 270 | 298 | 567 | 1.30E-131 | 479.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068385.t1 | Q9LSB9 | 47.9 | 282 | 129 | 3 | 1 | 270 | 480 | 755 | 1.2e-56 | 221.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068385.t1 | A0A072VDT5 | 97.8 | 270 | 6 | 0 | 1 | 270 | 298 | 567 | 9.2e-132 | 479.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049254 | MS.gene068385 | 0.843631 | 1.24E-58 | -1.69E-46 |
| MS.gene049272 | MS.gene068385 | 0.832718 | 8.06E-56 | -1.69E-46 |
| MS.gene049343 | MS.gene068385 | 0.803468 | 3.44E-49 | -1.69E-46 |
| MS.gene049378 | MS.gene068385 | 0.827066 | 1.92E-54 | -1.69E-46 |
| MS.gene049404 | MS.gene068385 | 0.829642 | 4.59E-55 | -1.69E-46 |
| MS.gene049435 | MS.gene068385 | 0.809711 | 1.65E-50 | -1.69E-46 |
| MS.gene049669 | MS.gene068385 | 0.848865 | 4.67E-60 | -1.69E-46 |
| MS.gene049825 | MS.gene068385 | 0.819199 | 1.32E-52 | -1.69E-46 |
| MS.gene049850 | MS.gene068385 | 0.805445 | 1.33E-49 | -1.69E-46 |
| MS.gene049873 | MS.gene068385 | 0.819646 | 1.04E-52 | -1.69E-46 |
| MS.gene050026 | MS.gene068385 | 0.83768 | 4.51E-57 | -1.69E-46 |
| MS.gene050119 | MS.gene068385 | 0.805294 | 1.43E-49 | -1.69E-46 |
| MS.gene050156 | MS.gene068385 | 0.837721 | 4.40E-57 | -1.69E-46 |
| MS.gene05016 | MS.gene068385 | 0.817123 | 3.88E-52 | -1.69E-46 |
| MS.gene050198 | MS.gene068385 | 0.804055 | 2.60E-49 | -1.69E-46 |
| MS.gene050201 | MS.gene068385 | 0.831517 | 1.60E-55 | -1.69E-46 |
| MS.gene050226 | MS.gene068385 | 0.809787 | 1.59E-50 | -1.69E-46 |
| MS.gene050227 | MS.gene068385 | 0.824573 | 7.50E-54 | -1.69E-46 |
| MS.gene050228 | MS.gene068385 | 0.828465 | 8.85E-55 | -1.69E-46 |
| MS.gene050303 | MS.gene068385 | 0.814298 | 1.65E-51 | -1.69E-46 |
| MS.gene050330 | MS.gene068385 | -0.807455 | 5.01E-50 | -1.69E-46 |
| MS.gene050355 | MS.gene068385 | 0.825376 | 4.85E-54 | -1.69E-46 |
| MS.gene050442 | MS.gene068385 | 0.854213 | 1.43E-61 | -1.69E-46 |
| MS.gene050559 | MS.gene068385 | 0.827317 | 1.67E-54 | -1.69E-46 |
| MS.gene050561 | MS.gene068385 | 0.82633 | 2.88E-54 | -1.69E-46 |
| MS.gene050562 | MS.gene068385 | 0.805741 | 1.15E-49 | -1.69E-46 |
| MS.gene050680 | MS.gene068385 | 0.827425 | 1.58E-54 | -1.69E-46 |
| MS.gene050719 | MS.gene068385 | 0.820759 | 5.79E-53 | -1.69E-46 |
| MS.gene050721 | MS.gene068385 | 0.819448 | 1.16E-52 | -1.69E-46 |
| MS.gene050724 | MS.gene068385 | 0.812294 | 4.56E-51 | -1.69E-46 |
| MS.gene050750 | MS.gene068385 | 0.850183 | 2.00E-60 | -1.69E-46 |
| MS.gene050780 | MS.gene068385 | 0.802674 | 5.02E-49 | -1.69E-46 |
| MS.gene050799 | MS.gene068385 | 0.800619 | 1.32E-48 | -1.69E-46 |
| MS.gene050807 | MS.gene068385 | 0.815518 | 8.87E-52 | -1.69E-46 |
| MS.gene050820 | MS.gene068385 | 0.801192 | 1.01E-48 | -1.69E-46 |
| MS.gene050844 | MS.gene068385 | 0.820862 | 5.48E-53 | -1.69E-46 |
| MS.gene050905 | MS.gene068385 | -0.800941 | 1.14E-48 | -1.69E-46 |
| MS.gene050953 | MS.gene068385 | 0.846971 | 1.55E-59 | -1.69E-46 |
| MS.gene051181 | MS.gene068385 | 0.814806 | 1.28E-51 | -1.69E-46 |
| MS.gene051198 | MS.gene068385 | 0.807359 | 5.25E-50 | -1.69E-46 |
| MS.gene051265 | MS.gene068385 | 0.815419 | 9.33E-52 | -1.69E-46 |
| MS.gene051368 | MS.gene068385 | 0.802835 | 4.65E-49 | -1.69E-46 |
| MS.gene051594 | MS.gene068385 | 0.829386 | 5.30E-55 | -1.69E-46 |
| MS.gene051744 | MS.gene068385 | 0.829399 | 5.26E-55 | -1.69E-46 |
| MS.gene051935 | MS.gene068385 | 0.823083 | 1.68E-53 | -1.69E-46 |
| MS.gene051957 | MS.gene068385 | 0.816523 | 5.29E-52 | -1.69E-46 |
| MS.gene052108 | MS.gene068385 | 0.816811 | 4.56E-52 | -1.69E-46 |
| MS.gene052176 | MS.gene068385 | 0.844107 | 9.28E-59 | -1.69E-46 |
| MS.gene052190 | MS.gene068385 | 0.837558 | 4.84E-57 | -1.69E-46 |
| MS.gene052208 | MS.gene068385 | 0.807625 | 4.61E-50 | -1.69E-46 |
| MS.gene052302 | MS.gene068385 | 0.803577 | 3.26E-49 | -1.69E-46 |
| MS.gene052333 | MS.gene068385 | 0.838722 | 2.43E-57 | -1.69E-46 |
| MS.gene052358 | MS.gene068385 | -0.843049 | 1.78E-58 | -1.69E-46 |
| MS.gene052376 | MS.gene068385 | 0.815347 | 9.69E-52 | -1.69E-46 |
| MS.gene052488 | MS.gene068385 | 0.800939 | 1.14E-48 | -1.69E-46 |
| MS.gene052519 | MS.gene068385 | 0.850917 | 1.25E-60 | -1.69E-46 |
| MS.gene052582 | MS.gene068385 | 0.832036 | 1.19E-55 | -1.69E-46 |
| MS.gene052584 | MS.gene068385 | 0.830058 | 3.64E-55 | -1.69E-46 |
| MS.gene052586 | MS.gene068385 | 0.821173 | 4.65E-53 | -1.69E-46 |
| MS.gene052611 | MS.gene068385 | 0.818628 | 1.78E-52 | -1.69E-46 |
| MS.gene052773 | MS.gene068385 | 0.819487 | 1.13E-52 | -1.69E-46 |
| MS.gene052860 | MS.gene068385 | 0.800851 | 1.19E-48 | -1.69E-46 |
| MS.gene052863 | MS.gene068385 | 0.822595 | 2.18E-53 | -1.69E-46 |
| MS.gene052867 | MS.gene068385 | 0.824918 | 6.22E-54 | -1.69E-46 |
| MS.gene052926 | MS.gene068385 | 0.813444 | 2.55E-51 | -1.69E-46 |
| MS.gene052954 | MS.gene068385 | 0.810867 | 9.31E-51 | -1.69E-46 |
| MS.gene053006 | MS.gene068385 | 0.81773 | 2.83E-52 | -1.69E-46 |
| MS.gene053054 | MS.gene068385 | -0.826333 | 2.87E-54 | -1.69E-46 |
| MS.gene053145 | MS.gene068385 | 0.812816 | 3.51E-51 | -1.69E-46 |
| MS.gene053166 | MS.gene068385 | 0.837321 | 5.57E-57 | -1.69E-46 |
| MS.gene053201 | MS.gene068385 | 0.82838 | 9.28E-55 | -1.69E-46 |
| MS.gene053202 | MS.gene068385 | 0.80373 | 3.03E-49 | -1.69E-46 |
| MS.gene053226 | MS.gene068385 | 0.820699 | 5.97E-53 | -1.69E-46 |
| MS.gene053294 | MS.gene068385 | 0.810222 | 1.28E-50 | -1.69E-46 |
| MS.gene053565 | MS.gene068385 | 0.806941 | 6.44E-50 | -1.69E-46 |
| MS.gene05365 | MS.gene068385 | 0.802398 | 5.72E-49 | -1.69E-46 |
| MS.gene053953 | MS.gene068385 | 0.821593 | 3.72E-53 | -1.69E-46 |
| MS.gene053966 | MS.gene068385 | 0.809543 | 1.80E-50 | -1.69E-46 |
| MS.gene053998 | MS.gene068385 | 0.813135 | 2.98E-51 | -1.69E-46 |
| MS.gene05414 | MS.gene068385 | 0.816886 | 4.39E-52 | -1.69E-46 |
| MS.gene05415 | MS.gene068385 | 0.853098 | 2.99E-61 | -1.69E-46 |
| MS.gene054160 | MS.gene068385 | 0.809344 | 1.98E-50 | -1.69E-46 |
| MS.gene054236 | MS.gene068385 | 0.804404 | 2.20E-49 | -1.69E-46 |
| MS.gene054291 | MS.gene068385 | -0.835947 | 1.25E-56 | -1.69E-46 |
| MS.gene054405 | MS.gene068385 | 0.836858 | 7.31E-57 | -1.69E-46 |
| MS.gene054467 | MS.gene068385 | 0.805141 | 1.54E-49 | -1.69E-46 |
| MS.gene054468 | MS.gene068385 | 0.837525 | 4.94E-57 | -1.69E-46 |
| MS.gene054472 | MS.gene068385 | 0.820165 | 7.92E-53 | -1.69E-46 |
| MS.gene054559 | MS.gene068385 | 0.841411 | 4.83E-58 | -1.69E-46 |
| MS.gene054576 | MS.gene068385 | 0.801234 | 9.91E-49 | -1.69E-46 |
| MS.gene054651 | MS.gene068385 | 0.833705 | 4.57E-56 | -1.69E-46 |
| MS.gene054657 | MS.gene068385 | 0.825855 | 3.73E-54 | -1.69E-46 |
| MS.gene05510 | MS.gene068385 | 0.804476 | 2.12E-49 | -1.69E-46 |
| MS.gene055166 | MS.gene068385 | 0.809441 | 1.89E-50 | -1.69E-46 |
| MS.gene055227 | MS.gene068385 | 0.806361 | 8.54E-50 | -1.69E-46 |
| MS.gene055228 | MS.gene068385 | 0.809576 | 1.77E-50 | -1.69E-46 |
| MS.gene055230 | MS.gene068385 | 0.803436 | 3.49E-49 | -1.69E-46 |
| MS.gene055356 | MS.gene068385 | 0.810376 | 1.19E-50 | -1.69E-46 |
| MS.gene055357 | MS.gene068385 | 0.81149 | 6.82E-51 | -1.69E-46 |
| MS.gene055358 | MS.gene068385 | 0.821969 | 3.04E-53 | -1.69E-46 |
| MS.gene055586 | MS.gene068385 | 0.812664 | 3.78E-51 | -1.69E-46 |
| MS.gene055589 | MS.gene068385 | 0.802018 | 6.85E-49 | -1.69E-46 |
| MS.gene055623 | MS.gene068385 | 0.80102 | 1.10E-48 | -1.69E-46 |
| MS.gene055701 | MS.gene068385 | 0.821495 | 3.92E-53 | -1.69E-46 |
| MS.gene055778 | MS.gene068385 | 0.822756 | 2.00E-53 | -1.69E-46 |
| MS.gene055818 | MS.gene068385 | 0.885842 | 5.74E-72 | -1.69E-46 |
| MS.gene055841 | MS.gene068385 | 0.801622 | 8.26E-49 | -1.69E-46 |
| MS.gene05604 | MS.gene068385 | 0.816771 | 4.66E-52 | -1.69E-46 |
| MS.gene056050 | MS.gene068385 | 0.819944 | 8.90E-53 | -1.69E-46 |
| MS.gene056162 | MS.gene068385 | 0.809557 | 1.78E-50 | -1.69E-46 |
| MS.gene056173 | MS.gene068385 | 0.802343 | 5.87E-49 | -1.69E-46 |
| MS.gene056174 | MS.gene068385 | 0.811321 | 7.42E-51 | -1.69E-46 |
| MS.gene056366 | MS.gene068385 | 0.853714 | 1.99E-61 | -1.69E-46 |
| MS.gene056373 | MS.gene068385 | 0.817378 | 3.40E-52 | -1.69E-46 |
| MS.gene056498 | MS.gene068385 | 0.823793 | 1.14E-53 | -1.69E-46 |
| MS.gene056506 | MS.gene068385 | 0.862016 | 6.83E-64 | -1.69E-46 |
| MS.gene056776 | MS.gene068385 | 0.812397 | 4.33E-51 | -1.69E-46 |
| MS.gene057021 | MS.gene068385 | 0.835985 | 1.22E-56 | -1.69E-46 |
| MS.gene057026 | MS.gene068385 | 0.862424 | 5.12E-64 | -1.69E-46 |
| MS.gene057027 | MS.gene068385 | 0.819683 | 1.02E-52 | -1.69E-46 |
| MS.gene057060 | MS.gene068385 | 0.87434 | 7.29E-68 | -1.69E-46 |
| MS.gene057200 | MS.gene068385 | 0.833448 | 5.30E-56 | -1.69E-46 |
| MS.gene057208 | MS.gene068385 | 0.821944 | 3.08E-53 | -1.69E-46 |
| MS.gene057314 | MS.gene068385 | 0.817591 | 3.05E-52 | -1.69E-46 |
| MS.gene057414 | MS.gene068385 | 0.833148 | 6.30E-56 | -1.69E-46 |
| MS.gene057444 | MS.gene068385 | 0.832891 | 7.30E-56 | -1.69E-46 |
| MS.gene057555 | MS.gene068385 | 0.801136 | 1.04E-48 | -1.69E-46 |
| MS.gene057598 | MS.gene068385 | 0.809619 | 1.73E-50 | -1.69E-46 |
| MS.gene057693 | MS.gene068385 | 0.805543 | 1.27E-49 | -1.69E-46 |
| MS.gene057869 | MS.gene068385 | 0.810479 | 1.13E-50 | -1.69E-46 |
| MS.gene05795 | MS.gene068385 | 0.818584 | 1.82E-52 | -1.69E-46 |
| MS.gene058008 | MS.gene068385 | 0.813819 | 2.11E-51 | -1.69E-46 |
| MS.gene058009 | MS.gene068385 | 0.826452 | 2.69E-54 | -1.69E-46 |
| MS.gene058037 | MS.gene068385 | 0.854838 | 9.43E-62 | -1.69E-46 |
| MS.gene058498 | MS.gene068385 | 0.802436 | 5.61E-49 | -1.69E-46 |
| MS.gene058502 | MS.gene068385 | 0.8188 | 1.62E-52 | -1.69E-46 |
| MS.gene058625 | MS.gene068385 | 0.853287 | 2.64E-61 | -1.69E-46 |
| MS.gene058635 | MS.gene068385 | 0.802232 | 6.19E-49 | -1.69E-46 |
| MS.gene058636 | MS.gene068385 | 0.816327 | 5.85E-52 | -1.69E-46 |
| MS.gene058829 | MS.gene068385 | 0.855577 | 5.75E-62 | -1.69E-46 |
| MS.gene058979 | MS.gene068385 | 0.829231 | 5.78E-55 | -1.69E-46 |
| MS.gene059015 | MS.gene068385 | 0.806497 | 8.00E-50 | -1.69E-46 |
| MS.gene059128 | MS.gene068385 | 0.807198 | 5.68E-50 | -1.69E-46 |
| MS.gene059161 | MS.gene068385 | 0.81099 | 8.76E-51 | -1.69E-46 |
| MS.gene059272 | MS.gene068385 | 0.811088 | 8.34E-51 | -1.69E-46 |
| MS.gene059317 | MS.gene068385 | 0.810168 | 1.32E-50 | -1.69E-46 |
| MS.gene059382 | MS.gene068385 | 0.821481 | 3.95E-53 | -1.69E-46 |
| MS.gene05954 | MS.gene068385 | 0.839798 | 1.28E-57 | -1.69E-46 |
| MS.gene059569 | MS.gene068385 | 0.816737 | 4.74E-52 | -1.69E-46 |
| MS.gene059584 | MS.gene068385 | 0.835797 | 1.36E-56 | -1.69E-46 |
| MS.gene059625 | MS.gene068385 | 0.800064 | 1.72E-48 | -1.69E-46 |
| MS.gene059789 | MS.gene068385 | 0.803837 | 2.88E-49 | -1.69E-46 |
| MS.gene05979 | MS.gene068385 | 0.801523 | 8.65E-49 | -1.69E-46 |
| MS.gene060011 | MS.gene068385 | 0.81072 | 1.00E-50 | -1.69E-46 |
| MS.gene060137 | MS.gene068385 | -0.802299 | 5.99E-49 | -1.69E-46 |
| MS.gene060322 | MS.gene068385 | 0.844016 | 9.82E-59 | -1.69E-46 |
| MS.gene060429 | MS.gene068385 | 0.800062 | 1.72E-48 | -1.69E-46 |
| MS.gene060521 | MS.gene068385 | 0.85164 | 7.78E-61 | -1.69E-46 |
| MS.gene060553 | MS.gene068385 | 0.836809 | 7.53E-57 | -1.69E-46 |
| MS.gene060572 | MS.gene068385 | 0.867076 | 1.79E-65 | -1.69E-46 |
| MS.gene060634 | MS.gene068385 | -0.842448 | 2.57E-58 | -1.69E-46 |
| MS.gene060683 | MS.gene068385 | 0.809801 | 1.58E-50 | -1.69E-46 |
| MS.gene060685 | MS.gene068385 | 0.813448 | 2.55E-51 | -1.69E-46 |
| MS.gene060781 | MS.gene068385 | 0.811247 | 7.70E-51 | -1.69E-46 |
| MS.gene060803 | MS.gene068385 | 0.807562 | 4.76E-50 | -1.69E-46 |
| MS.gene060879 | MS.gene068385 | 0.826581 | 2.51E-54 | -1.69E-46 |
| MS.gene060918 | MS.gene068385 | 0.836743 | 7.82E-57 | -1.69E-46 |
| MS.gene060919 | MS.gene068385 | 0.802531 | 5.37E-49 | -1.69E-46 |
| MS.gene061040 | MS.gene068385 | -0.864326 | 1.32E-64 | -1.69E-46 |
| MS.gene061114 | MS.gene068385 | -0.808762 | 2.64E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068385.t1 | MTR_2g104070 | 97.778 | 270 | 6 | 0 | 1 | 270 | 435 | 704 | 0.0 | 529 |
| MS.gene068385.t1 | MTR_2g104070 | 97.778 | 270 | 6 | 0 | 1 | 270 | 298 | 567 | 0.0 | 526 |
| MS.gene068385.t1 | MTR_3g106770 | 48.387 | 248 | 127 | 1 | 1 | 247 | 336 | 583 | 9.42e-64 | 209 |
| MS.gene068385.t1 | MTR_3g106770 | 48.387 | 248 | 127 | 1 | 1 | 247 | 454 | 701 | 7.80e-63 | 209 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068385.t1 | AT3G15920 | 48.936 | 282 | 126 | 3 | 1 | 270 | 480 | 755 | 1.17e-78 | 253 |
| MS.gene068385.t1 | AT4G32160 | 46.614 | 251 | 127 | 3 | 1 | 248 | 450 | 696 | 2.52e-55 | 189 |
| MS.gene068385.t1 | AT2G25350 | 35.470 | 234 | 120 | 5 | 1 | 231 | 438 | 643 | 4.21e-31 | 122 |
| MS.gene068385.t1 | AT2G25350 | 40.000 | 160 | 96 | 0 | 1 | 160 | 438 | 597 | 1.07e-30 | 120 |
| MS.gene068385.t1 | AT2G25350 | 43.443 | 122 | 69 | 0 | 1 | 122 | 438 | 559 | 9.32e-25 | 103 |
| MS.gene068385.t1 | AT2G25350 | 43.443 | 122 | 69 | 0 | 1 | 122 | 438 | 559 | 9.32e-25 | 103 |
Find 43 sgRNAs with CRISPR-Local
Find 107 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGTGGACAATGCCAGTTTA+AGG | 0.185325 | 2.3:+918967 | MS.gene068385:CDS |
| ATTGACTTGTTTCCTTAAAC+TGG | 0.308319 | 2.3:-918979 | None:intergenic |
| TACTTCACCATCTGATGCTT+TGG | 0.326539 | 2.3:+918268 | MS.gene068385:CDS |
| TACACAAATGCAGTGGGATA+TGG | 0.344319 | 2.3:+917439 | MS.gene068385:CDS |
| TACAAAAGCATCCGTTGTTC+AGG | 0.349368 | 2.3:+917618 | MS.gene068385:CDS |
| GGACAACTAATGAAGAATTA+AGG | 0.355410 | 2.3:+918925 | MS.gene068385:CDS |
| TATACAACTACAGATGATAC+TGG | 0.355565 | 2.3:+918903 | MS.gene068385:CDS |
| TGTTAGCAACATCAGATAAT+CGG | 0.359290 | 2.3:+918294 | MS.gene068385:CDS |
| AGATATGAAGTTGCTAATAA+AGG | 0.405971 | 2.3:+917735 | MS.gene068385:CDS |
| GCAACAGCTCAGCGAATTGA+TGG | 0.429244 | 2.3:+917795 | MS.gene068385:CDS |
| TTCATCTTCCTCATTCTCCT+CGG | 0.464822 | 2.3:-919027 | None:intergenic |
| TTATAATGATTTAGGATGAA+AGG | 0.489862 | 2.3:+917581 | MS.gene068385:intron |
| ATGGGATCAGGCACAGCTCT+TGG | 0.494460 | 2.3:+918842 | MS.gene068385:intron |
| GAGAACTCTTCAGAAGGAAA+AGG | 0.505418 | 2.3:+918121 | MS.gene068385:intron |
| GTTCTAACTGAGAGCTTCTG+AGG | 0.530046 | 2.3:-917768 | None:intergenic |
| TGAAGAATTAAGGAAAATGC+TGG | 0.530259 | 2.3:+918935 | MS.gene068385:CDS |
| ACTCTTGAAGCCTCTTCTGA+AGG | 0.541023 | 2.3:-918199 | None:intergenic |
| AATGAAATTGAAGTTTGAAG+AGG | 0.542626 | 2.3:+917493 | MS.gene068385:CDS |
| CTGCAGAGAAAACATGCAGC+AGG | 0.543507 | 2.3:+917391 | None:intergenic |
| TCAATTTCATTTCTGTCTCG+AGG | 0.553699 | 2.3:-917481 | None:intergenic |
| GCAACATCAGATAATCGGAT+TGG | 0.554769 | 2.3:+918299 | MS.gene068385:CDS |
| TCTCAAATCCGAGGAGAATG+AGG | 0.568189 | 2.3:+919019 | MS.gene068385:CDS |
| TTCTAACTGAGAGCTTCTGA+GGG | 0.594772 | 2.3:-917767 | None:intergenic |
| TATTCAGAGAACTCTTCAGA+AGG | 0.594795 | 2.3:+918115 | MS.gene068385:intron |
| AACAAATCCAAAGCATCAGA+TGG | 0.605868 | 2.3:-918275 | None:intergenic |
| ACAAATGCAGTGGGATATGG+AGG | 0.618627 | 2.3:+917442 | MS.gene068385:CDS |
| AGTGTGCAATCCTTCAGAAG+AGG | 0.619022 | 2.3:+918189 | MS.gene068385:CDS |
| AAAGGTTTACACAAATGCAG+TGG | 0.626560 | 2.3:+917432 | MS.gene068385:CDS |
| AAGGTTTACACAAATGCAGT+GGG | 0.626811 | 2.3:+917433 | MS.gene068385:CDS |
| AGTGGGATATGGAGGAACTT+CGG | 0.629220 | 2.3:+917450 | MS.gene068385:CDS |
| CATTTGCTTCTCCTGAACAA+CGG | 0.635355 | 2.3:-917629 | None:intergenic |
| ATACAACTACAGATGATACT+GGG | 0.636328 | 2.3:+918904 | MS.gene068385:CDS |
| GATTGGTCTCTTGCTTGCAG+AGG | 0.641568 | 2.3:+918316 | MS.gene068385:CDS |
| TCTCCTCGGATTTGAGAGCA+CGG | 0.646545 | 2.3:-919013 | None:intergenic |
| TTAGGATGAAAGGTTGCTAG+CGG | 0.654903 | 2.3:+917591 | MS.gene068385:intron |
| CCGCCGTGCTCTCAAATCCG+AGG | 0.655129 | 2.3:+919010 | MS.gene068385:CDS |
| ACAAGATGTAGAAAATGTTG+TGG | 0.656733 | 2.3:+918866 | MS.gene068385:CDS |
| ACAGCTCAGCGAATTGATGG+AGG | 0.664421 | 2.3:+917798 | MS.gene068385:CDS |
| AATGTTGTGGATGCTGCGGA+AGG | 0.680763 | 2.3:+918879 | MS.gene068385:CDS |
| GGGATATGGAGGAACTTCGG+AGG | 0.692756 | 2.3:+917453 | MS.gene068385:CDS |
| GTTGCAGCAAGAGCTAGATG+TGG | 0.705517 | 2.3:+917651 | MS.gene068385:CDS |
| AGAAAATGTTGTGGATGCTG+CGG | 0.718543 | 2.3:+918875 | MS.gene068385:CDS |
| CCTCGGATTTGAGAGCACGG+CGG | 0.772593 | 2.3:-919010 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TAACAATTTATTATTTTCAT+TGG | + | chr2.3:918520-918539 | MS.gene068385:intron | 10.0% |
| !! | GTGAAAAATTATAATGATTT+AGG | + | chr2.3:917573-917592 | MS.gene068385:intron | 15.0% |
| !!! | AATTGGATTTTCAAATTTTT+AGG | - | chr2.3:918067-918086 | None:intergenic | 15.0% |
| !!! | TCATTATAATTTTTCACTAT+AGG | - | chr2.3:917570-917589 | None:intergenic | 15.0% |
| !!! | TTTGTACGAAAAATATTTTT+CGG | + | chr2.3:918350-918369 | MS.gene068385:intron | 15.0% |
| !!! | TTTTATATCTGCAAATATAT+GGG | + | chr2.3:918824-918843 | MS.gene068385:intron | 15.0% |
| !!! | TTTTTATATCTGCAAATATA+TGG | + | chr2.3:918823-918842 | MS.gene068385:intron | 15.0% |
| !! | AACAAAAACATGAAGTTAAT+TGG | - | chr2.3:918084-918103 | None:intergenic | 20.0% |
| !!! | AGTTCTAAAATAAGTAGAAT+AGG | - | chr2.3:918471-918490 | None:intergenic | 20.0% |
| !!! | AGTTTTAATCTTTACTTTCT+CGG | + | chr2.3:918392-918411 | MS.gene068385:intron | 20.0% |
| !!! | GTTCTAAAATAAGTAGAATA+GGG | - | chr2.3:918470-918489 | None:intergenic | 20.0% |
| !!! | TTAGAAAAGTTTTTTGCAAA+TGG | - | chr2.3:919055-919074 | None:intergenic | 20.0% |
| !!! | TTATAATGATTTAGGATGAA+AGG | + | chr2.3:917581-917600 | MS.gene068385:intron | 20.0% |
| !!! | TTATTTTAGAACTCCTATTT+TGG | + | chr2.3:918478-918497 | MS.gene068385:intron | 20.0% |
| !!! | TTCTAAAATAAGTAGAATAG+GGG | - | chr2.3:918469-918488 | None:intergenic | 20.0% |
| !!! | TTGTACGAAAAATATTTTTC+GGG | + | chr2.3:918351-918370 | MS.gene068385:intron | 20.0% |
| ! | AAATTTCAGAATCTTGTCAT+AGG | - | chr2.3:918717-918736 | None:intergenic | 25.0% |
| ! | AATGAAATTGAAGTTTGAAG+AGG | + | chr2.3:917493-917512 | MS.gene068385:CDS | 25.0% |
| ! | AGATATGAAGTTGCTAATAA+AGG | + | chr2.3:917735-917754 | MS.gene068385:CDS | 25.0% |
| ! | ATAAAAAACTGTGAGTAAAG+AGG | - | chr2.3:918810-918829 | None:intergenic | 25.0% |
| ! | CAAATTATCATAGATTCATG+CGG | - | chr2.3:918630-918649 | None:intergenic | 25.0% |
| ! | TAACATATAAGAACCAGAAA+GGG | - | chr2.3:917847-917866 | None:intergenic | 25.0% |
| ! | TGAAAAAACTCAAATTTGAC+AGG | - | chr2.3:918667-918686 | None:intergenic | 25.0% |
| !! | TAACATCTCTTTTCACATTT+TGG | + | chr2.3:917541-917560 | MS.gene068385:intron | 25.0% |
| !! | TGTAAATTTTCTTGTTGAAG+AGG | + | chr2.3:918226-918245 | MS.gene068385:CDS | 25.0% |
| !! | TGTACGAAAAATATTTTTCG+GGG | + | chr2.3:918352-918371 | MS.gene068385:intron | 25.0% |
| !!! | GGTTTAGATTTTCTGTTTTT+AGG | + | chr2.3:918038-918057 | MS.gene068385:intron | 25.0% |
| !!! | GTTTTAATCTTTACTTTCTC+GGG | + | chr2.3:918393-918412 | MS.gene068385:intron | 25.0% |
| ACAAGATGTAGAAAATGTTG+TGG | + | chr2.3:918866-918885 | MS.gene068385:CDS | 30.0% | |
| ACATTTCTCTAAACTAGACT+GGG | + | chr2.3:917890-917909 | MS.gene068385:intron | 30.0% | |
| AGAGTAACACAATTGATGTA+GGG | + | chr2.3:918584-918603 | MS.gene068385:intron | 30.0% | |
| ATACAACTACAGATGATACT+GGG | + | chr2.3:918904-918923 | MS.gene068385:CDS | 30.0% | |
| ATTCTTGATCCTTCTACTAA+AGG | + | chr2.3:918008-918027 | MS.gene068385:intron | 30.0% | |
| CAGAGTGTAATATCCAAAAT+AGG | - | chr2.3:918494-918513 | None:intergenic | 30.0% | |
| CCTTCTACTAAAGGAAAAAA+TGG | + | chr2.3:918017-918036 | MS.gene068385:intron | 30.0% | |
| GAATCTTGTCATAGGAAAAT+TGG | - | chr2.3:918709-918728 | None:intergenic | 30.0% | |
| GGACAACTAATGAAGAATTA+AGG | + | chr2.3:918925-918944 | MS.gene068385:CDS | 30.0% | |
| GTAACATATAAGAACCAGAA+AGG | - | chr2.3:917848-917867 | None:intergenic | 30.0% | |
| TATACAACTACAGATGATAC+TGG | + | chr2.3:918903-918922 | MS.gene068385:CDS | 30.0% | |
| TCACCTAGCAATTATCATTT+GGG | + | chr2.3:918736-918755 | MS.gene068385:intron | 30.0% | |
| TGAAGAATTAAGGAAAATGC+TGG | + | chr2.3:918935-918954 | MS.gene068385:CDS | 30.0% | |
| TGTTAGCAACATCAGATAAT+CGG | + | chr2.3:918294-918313 | MS.gene068385:CDS | 30.0% | |
| TTCACCTAGCAATTATCATT+TGG | + | chr2.3:918735-918754 | MS.gene068385:intron | 30.0% | |
| ! | TTGTGTAAACCTTTCCTTTT+CGG | - | chr2.3:917426-917445 | None:intergenic | 30.0% |
| !! | AATGCTGGCTAATATTTTTG+TGG | + | chr2.3:918950-918969 | MS.gene068385:CDS | 30.0% |
| !! | ATTGACTTGTTTCCTTAAAC+TGG | - | chr2.3:918982-919001 | None:intergenic | 30.0% |
| !!! | CCATTTTTTCCTTTAGTAGA+AGG | - | chr2.3:918020-918039 | None:intergenic | 30.0% |
| AAAAAGTAGATGTGGAGGTA+TGG | + | chr2.3:917821-917840 | MS.gene068385:intron | 35.0% | |
| AAAGGCAGCTTGTAAATTTG+CGG | - | chr2.3:918432-918451 | None:intergenic | 35.0% | |
| AAAGGTTTACACAAATGCAG+TGG | + | chr2.3:917432-917451 | MS.gene068385:CDS | 35.0% | |
| AACTTAAACACGTAGTCAGA+TGG | - | chr2.3:917934-917953 | None:intergenic | 35.0% | |
| AAGGTTTACACAAATGCAGT+GGG | + | chr2.3:917433-917452 | MS.gene068385:CDS | 35.0% | |
| ATCTGCAAATATATGGGATC+AGG | + | chr2.3:918830-918849 | MS.gene068385:intron | 35.0% | |
| CAACCCAAATGATAATTGCT+AGG | - | chr2.3:918742-918761 | None:intergenic | 35.0% | |
| CAGAGTAACACAATTGATGT+AGG | + | chr2.3:918583-918602 | MS.gene068385:intron | 35.0% | |
| GACATTTCTCTAAACTAGAC+TGG | + | chr2.3:917889-917908 | MS.gene068385:intron | 35.0% | |
| GCTGCCTTTACAATCTATAA+CGG | + | chr2.3:918443-918462 | MS.gene068385:intron | 35.0% | |
| TAAAGAGGACACCATATAGA+AGG | - | chr2.3:918795-918814 | None:intergenic | 35.0% | |
| TATTCAGAGAACTCTTCAGA+AGG | + | chr2.3:918115-918134 | MS.gene068385:intron | 35.0% | |
| TCAATTTCATTTCTGTCTCG+AGG | - | chr2.3:917484-917503 | None:intergenic | 35.0% | |
| TTTCACTAGAACCATGCAAA+AGG | + | chr2.3:918550-918569 | MS.gene068385:intron | 35.0% | |
| ! | AACAAATCCAAAGCATCAGA+TGG | - | chr2.3:918278-918297 | None:intergenic | 35.0% |
| ! | CATCTCTTTTCACATTTTGG+TGG | + | chr2.3:917544-917563 | MS.gene068385:intron | 35.0% |
| ! | CTACATCAATTGTGTTACTC+TGG | - | chr2.3:918585-918604 | None:intergenic | 35.0% |
| !!! | AAAATATTTTTCGGGGCACT+TGG | + | chr2.3:918359-918378 | MS.gene068385:intron | 35.0% |
| !!! | CTGTTTTAACCGAAAAGGAA+AGG | + | chr2.3:917414-917433 | MS.gene068385:CDS | 35.0% |
| ACACCATATAGAAGGCTAAG+GGG | - | chr2.3:918787-918806 | None:intergenic | 40.0% | |
| ACTAGAACCATGCAAAAGGA+CGG | + | chr2.3:918554-918573 | MS.gene068385:intron | 40.0% | |
| AGAAAATGTTGTGGATGCTG+CGG | + | chr2.3:918875-918894 | MS.gene068385:CDS | 40.0% | |
| CATTTGCTTCTCCTGAACAA+CGG | - | chr2.3:917632-917651 | None:intergenic | 40.0% | |
| GACACCATATAGAAGGCTAA+GGG | - | chr2.3:918788-918807 | None:intergenic | 40.0% | |
| GACTGGGAAAGTAATTTGAC+TGG | + | chr2.3:917906-917925 | MS.gene068385:intron | 40.0% | |
| GAGAACTCTTCAGAAGGAAA+AGG | + | chr2.3:918121-918140 | MS.gene068385:intron | 40.0% | |
| GATGGAGGAAAAAGTAGATG+TGG | + | chr2.3:917813-917832 | MS.gene068385:CDS | 40.0% | |
| GCAACATCAGATAATCGGAT+TGG | + | chr2.3:918299-918318 | MS.gene068385:CDS | 40.0% | |
| TACACAAATGCAGTGGGATA+TGG | + | chr2.3:917439-917458 | MS.gene068385:CDS | 40.0% | |
| TACTTCACCATCTGATGCTT+TGG | + | chr2.3:918268-918287 | MS.gene068385:CDS | 40.0% | |
| TAGGAAAATTGGAGTGTGTC+TGG | - | chr2.3:918698-918717 | None:intergenic | 40.0% | |
| TTAGGATGAAAGGTTGCTAG+CGG | + | chr2.3:917591-917610 | MS.gene068385:intron | 40.0% | |
| TTCATCTTCCTCATTCTCCT+CGG | - | chr2.3:919030-919049 | None:intergenic | 40.0% | |
| TTCTAACTGAGAGCTTCTGA+GGG | - | chr2.3:917770-917789 | None:intergenic | 40.0% | |
| TTGTGGACAATGCCAGTTTA+AGG | + | chr2.3:918967-918986 | MS.gene068385:CDS | 40.0% | |
| !! | TACAAAAGCATCCGTTGTTC+AGG | + | chr2.3:917618-917637 | MS.gene068385:CDS | 40.0% |
| ACAAATGCAGTGGGATATGG+AGG | + | chr2.3:917442-917461 | MS.gene068385:CDS | 45.0% | |
| ACTCTTGAAGCCTCTTCTGA+AGG | - | chr2.3:918202-918221 | None:intergenic | 45.0% | |
| AGTGGGATATGGAGGAACTT+CGG | + | chr2.3:917450-917469 | MS.gene068385:CDS | 45.0% | |
| AGTGTGCAATCCTTCAGAAG+AGG | + | chr2.3:918189-918208 | MS.gene068385:CDS | 45.0% | |
| GGACACCATATAGAAGGCTA+AGG | - | chr2.3:918789-918808 | None:intergenic | 45.0% | |
| GGAGGAAAAAGTAGATGTGG+AGG | + | chr2.3:917816-917835 | MS.gene068385:CDS | 45.0% | |
| GGGAAGCAAGCAGTAAAACA+TGG | - | chr2.3:918767-918786 | None:intergenic | 45.0% | |
| GGGGCCGTTATAGATTGTAA+AGG | - | chr2.3:918450-918469 | None:intergenic | 45.0% | |
| GTTCTAACTGAGAGCTTCTG+AGG | - | chr2.3:917771-917790 | None:intergenic | 45.0% | |
| TCTCAAATCCGAGGAGAATG+AGG | + | chr2.3:919019-919038 | MS.gene068385:CDS | 45.0% | |
| ! | CTTCCCCTTAGCCTTCTATA+TGG | + | chr2.3:918781-918800 | MS.gene068385:intron | 45.0% |
| ! | GGAAAATCCGTCCTTTTGCA+TGG | - | chr2.3:918564-918583 | None:intergenic | 45.0% |
| !!! | GCAGGCTGTTTTAACCGAAA+AGG | + | chr2.3:917409-917428 | MS.gene068385:CDS | 45.0% |
| AATGTTGTGGATGCTGCGGA+AGG | + | chr2.3:918879-918898 | MS.gene068385:CDS | 50.0% | |
| ACAGCTCAGCGAATTGATGG+AGG | + | chr2.3:917798-917817 | MS.gene068385:CDS | 50.0% | |
| GCAACAGCTCAGCGAATTGA+TGG | + | chr2.3:917795-917814 | MS.gene068385:CDS | 50.0% | |
| GTTGCAGCAAGAGCTAGATG+TGG | + | chr2.3:917651-917670 | MS.gene068385:CDS | 50.0% | |
| TCTCCTCGGATTTGAGAGCA+CGG | - | chr2.3:919016-919035 | None:intergenic | 50.0% | |
| !! | GATTGGTCTCTTGCTTGCAG+AGG | + | chr2.3:918316-918335 | MS.gene068385:CDS | 50.0% |
| ATGGGATCAGGCACAGCTCT+TGG | + | chr2.3:918842-918861 | MS.gene068385:intron | 55.0% | |
| GGGATATGGAGGAACTTCGG+AGG | + | chr2.3:917453-917472 | MS.gene068385:CDS | 55.0% | |
| ! | TGTGGAGGTATGGCCCTTTC+TGG | + | chr2.3:917831-917850 | MS.gene068385:intron | 55.0% |
| ! | CCTCGGATTTGAGAGCACGG+CGG | - | chr2.3:919013-919032 | None:intergenic | 60.0% |
| CCGCCGTGCTCTCAAATCCG+AGG | + | chr2.3:919010-919029 | MS.gene068385:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.3 | gene | 917404 | 919094 | 917404 | ID=MS.gene068385 |
| chr2.3 | mRNA | 917404 | 919094 | 917404 | ID=MS.gene068385.t1;Parent=MS.gene068385 |
| chr2.3 | exon | 917404 | 917514 | 917404 | ID=MS.gene068385.t1.exon1;Parent=MS.gene068385.t1 |
| chr2.3 | CDS | 917404 | 917514 | 917404 | ID=cds.MS.gene068385.t1;Parent=MS.gene068385.t1 |
| chr2.3 | exon | 917595 | 917837 | 917595 | ID=MS.gene068385.t1.exon2;Parent=MS.gene068385.t1 |
| chr2.3 | CDS | 917595 | 917837 | 917595 | ID=cds.MS.gene068385.t1;Parent=MS.gene068385.t1 |
| chr2.3 | exon | 918122 | 918337 | 918122 | ID=MS.gene068385.t1.exon3;Parent=MS.gene068385.t1 |
| chr2.3 | CDS | 918122 | 918337 | 918122 | ID=cds.MS.gene068385.t1;Parent=MS.gene068385.t1 |
| chr2.3 | exon | 918852 | 919094 | 918852 | ID=MS.gene068385.t1.exon4;Parent=MS.gene068385.t1 |
| chr2.3 | CDS | 918852 | 919094 | 918852 | ID=cds.MS.gene068385.t1;Parent=MS.gene068385.t1 |
| Gene Sequence |
| Protein sequence |
