AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene07344


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene07344.t1 MTR_1g086430 85.484 62 8 1 1 62 38 98 6.75e-33 109
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 11 sgRNAs with CRISPR-Local

Find 11 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAAATGAGTGAAAATATTA+AGG 0.307429 1.2:+65104399 MS.gene07344:CDS
GAGGAAGATGACACGGAAAA+AGG 0.323663 1.2:+65104529 MS.gene07344:CDS
AAAATAAAGTTGTTGGTGTT+TGG 0.337332 1.2:+65104425 MS.gene07344:CDS
AAGGAAGAAAATAAAGTTGT+TGG 0.406793 1.2:+65104418 MS.gene07344:CDS
CTTGAAGATTTCGCTGAAGA+GGG 0.506298 1.2:+65104463 MS.gene07344:CDS
ACTTGAAGATTTCGCTGAAG+AGG 0.542202 1.2:+65104462 MS.gene07344:CDS
TGCGTGAGACACGTGAGCGT+TGG 0.555623 1.2:+65104371 MS.gene07344:CDS
TAACTGTGCTAATATTCTTG+AGG 0.568361 1.2:+65104510 MS.gene07344:CDS
AAATCTTCAAGTTTAAACGT+TGG 0.594153 1.2:-65104451 None:intergenic
GAAGATTTCGCTGAAGAGGG+TGG 0.633634 1.2:+65104466 MS.gene07344:CDS
TATTCTTGAGGAAGATGACA+CGG 0.661009 1.2:+65104522 MS.gene07344:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAAAATGAGTGAAAATATTA+AGG + chr1.2:65104399-65104418 MS.gene07344:CDS 20.0%
! AAGGAAGAAAATAAAGTTGT+TGG + chr1.2:65104418-65104437 MS.gene07344:CDS 25.0%
!! AAAATAAAGTTGTTGGTGTT+TGG + chr1.2:65104425-65104444 MS.gene07344:CDS 25.0%
!! AAATCTTCAAGTTTAAACGT+TGG - chr1.2:65104454-65104473 None:intergenic 25.0%
TAACTGTGCTAATATTCTTG+AGG + chr1.2:65104510-65104529 MS.gene07344:CDS 30.0%
TATTCTTGAGGAAGATGACA+CGG + chr1.2:65104522-65104541 MS.gene07344:CDS 35.0%
ACTTGAAGATTTCGCTGAAG+AGG + chr1.2:65104462-65104481 MS.gene07344:CDS 40.0%
CTTGAAGATTTCGCTGAAGA+GGG + chr1.2:65104463-65104482 MS.gene07344:CDS 40.0%
GAGGAAGATGACACGGAAAA+AGG + chr1.2:65104529-65104548 MS.gene07344:CDS 45.0%
GAAGATTTCGCTGAAGAGGG+TGG + chr1.2:65104466-65104485 MS.gene07344:CDS 50.0%
TGCGTGAGACACGTGAGCGT+TGG + chr1.2:65104371-65104390 MS.gene07344:CDS 60.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 65104370 65104576 65104370 ID=MS.gene07344
chr1.2 mRNA 65104370 65104576 65104370 ID=MS.gene07344.t1;Parent=MS.gene07344
chr1.2 exon 65104370 65104576 65104370 ID=MS.gene07344.t1.exon1;Parent=MS.gene07344.t1
chr1.2 CDS 65104370 65104576 65104370 ID=cds.MS.gene07344.t1;Parent=MS.gene07344.t1
Gene Sequence

>MS.gene07344

ATGCGTGAGACACGTGAGCGTTGGAAGAACAAAATGAGTGAAAATATTAAGGAAGAAAATAAAGTTGTTGGTGTTTGGAAGCCAACGTTTAAACTTGAAGATTTCGCTGAAGAGGGTGGTGCATCTAAGAACAATAATATTAACTGTGCTAATATTCTTGAGGAAGATGACACGGAAAAAGGTATTAACTTGAAGCTCTCACTTTGA

Protein sequence

>MS.gene07344.t1

MRETRERWKNKMSENIKEENKVVGVWKPTFKLEDFAEEGGASKNNNINCANILEEDDTEKGINLKLSL