AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene25151


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene25151.t1 MTR_8g020200 98.246 114 2 0 1 114 1 114 5.25e-76 220
MS.gene25151.t1 MTR_2g041310 71.053 114 33 0 1 114 1 114 6.06e-55 167
MS.gene25151.t1 MTR_4g125590 70.796 113 32 1 1 112 1 113 9.38e-54 164
MS.gene25151.t1 MTR_3g070490 46.250 80 43 0 6 85 36 115 1.41e-21 83.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene25151.t1 AT1G17455 70.796 113 33 0 1 113 1 113 4.47e-53 162
MS.gene25151.t1 AT1G17455 70.796 113 33 0 1 113 1 113 4.47e-53 162
MS.gene25151.t1 AT1G72630 66.667 114 37 1 1 113 5 118 5.50e-49 152
MS.gene25151.t1 AT2G06255 60.870 115 38 3 1 114 1 109 4.65e-42 134
MS.gene25151.t1 AT2G29950 52.113 71 34 0 16 86 34 104 1.36e-18 75.5
MS.gene25151.t1 AT2G40080 47.143 70 37 0 16 85 22 91 1.39e-18 75.1

Find 15 sgRNAs with CRISPR-Local

Find 17 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAATCCTAGACTCATGATTT+TGG 0.224769 8.4:-73221518 None:intergenic
GGTGATATATTTGGAGAATT+AGG 0.240535 8.4:+73221390 MS.gene25151:CDS
AACTTGATCTAATTCTCTTC+TGG 0.282924 8.4:-73221704 None:intergenic
AAAATGGAAGGTGATATATT+TGG 0.290272 8.4:+73221381 None:intergenic
ACATTTCTACTCAGATTATC+AGG 0.344511 8.4:-73221540 None:intergenic
AGACGAAGCTTCCCGCGACT+TGG 0.462270 8.4:-73221637 None:intergenic
AGTGGAACTCTCAAATCTGA+TGG 0.488717 8.4:+73221672 MS.gene25151:CDS
TAAACCAAAATCATGAGTCT+AGG 0.526715 8.4:+73221514 MS.gene25151:CDS
TTATCTGGTTGATTAAAAGC+CGG 0.545205 8.4:-73221494 None:intergenic
TCGCGGGAAGCTTCGTCTGA+AGG 0.551915 8.4:+73221642 MS.gene25151:CDS
GATAATCTGAGTAGAAATGT+TGG 0.559396 8.4:+73221543 MS.gene25151:CDS
GAGTTCGTTTACCAAGTCGC+GGG 0.624410 8.4:+73221626 MS.gene25151:CDS
CGAGTTCGTTTACCAAGTCG+CGG 0.624955 8.4:+73221625 MS.gene25151:CDS
TCGTCTGAAGGAGATTCAAG+TGG 0.640398 8.4:+73221654 MS.gene25151:CDS
GAATAGTAATATCAGAAGAG+TGG 0.647250 8.4:+73221581 MS.gene25151:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAGATATTTTGAATCAAAAC+CGG + chr8.4:73221475-73221494 MS.gene25151:CDS 20.0%
AACTTGATCTAATTCTCTTC+TGG - chr8.4:73221707-73221726 None:intergenic 30.0%
ACATTTCTACTCAGATTATC+AGG - chr8.4:73221543-73221562 None:intergenic 30.0%
GAATAGTAATATCAGAAGAG+TGG + chr8.4:73221581-73221600 MS.gene25151:CDS 30.0%
GATAATCTGAGTAGAAATGT+TGG + chr8.4:73221543-73221562 MS.gene25151:CDS 30.0%
GGTGATATATTTGGAGAATT+AGG + chr8.4:73221390-73221409 MS.gene25151:CDS 30.0%
TAAACCAAAATCATGAGTCT+AGG + chr8.4:73221514-73221533 MS.gene25151:CDS 30.0%
TTATCTGGTTGATTAAAAGC+CGG - chr8.4:73221497-73221516 None:intergenic 30.0%
! AGTAGATAGTAGAGTTTTAC+AGG + chr8.4:73221425-73221444 MS.gene25151:CDS 30.0%
!!! ACTCATGATTTTGGTTTATC+TGG - chr8.4:73221512-73221531 None:intergenic 30.0%
! GAATCCTAGACTCATGATTT+TGG - chr8.4:73221521-73221540 None:intergenic 35.0%
AGTGGAACTCTCAAATCTGA+TGG + chr8.4:73221672-73221691 MS.gene25151:CDS 40.0%
TCGTCTGAAGGAGATTCAAG+TGG + chr8.4:73221654-73221673 MS.gene25151:CDS 45.0%
CGAGTTCGTTTACCAAGTCG+CGG + chr8.4:73221625-73221644 MS.gene25151:CDS 50.0%
GAGTTCGTTTACCAAGTCGC+GGG + chr8.4:73221626-73221645 MS.gene25151:CDS 50.0%
AGACGAAGCTTCCCGCGACT+TGG - chr8.4:73221640-73221659 None:intergenic 60.0%
TCGCGGGAAGCTTCGTCTGA+AGG + chr8.4:73221642-73221661 MS.gene25151:CDS 60.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 73221384 73221728 73221384 ID=MS.gene25151
chr8.4 mRNA 73221384 73221728 73221384 ID=MS.gene25151.t1;Parent=MS.gene25151
chr8.4 exon 73221384 73221728 73221384 ID=MS.gene25151.t1.exon1;Parent=MS.gene25151.t1
chr8.4 CDS 73221384 73221728 73221384 ID=cds.MS.gene25151.t1;Parent=MS.gene25151.t1
Gene Sequence

>MS.gene25151

ATGGAAGGTGATATATTTGGAGAATTAGGTGATACAAGTCAAGTAGATAGTAGAGTTTTACAGGTATTTCAGAAGAGCTTAGTACAAGCACAAGATATTTTGAATCAAAACCGGCTTTTAATCAACCAGATAAACCAAAATCATGAGTCTAGGATTCCTGATAATCTGAGTAGAAATGTTGGTTTGATTAAAGAGCTGAATAGTAATATCAGAAGAGTGGTTGATCTTTATGCTGATCTTTCGAGTTCGTTTACCAAGTCGCGGGAAGCTTCGTCTGAAGGAGATTCAAGTGGAACTCTCAAATCTGATGGAAAAGTTAACCAGAAGAGAATTAGATCAAGTTAA

Protein sequence

>MS.gene25151.t1

MEGDIFGELGDTSQVDSRVLQVFQKSLVQAQDILNQNRLLINQINQNHESRIPDNLSRNVGLIKELNSNIRRVVDLYADLSSSFTKSREASSEGDSSGTLKSDGKVNQKRIRSS