Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44986.t1 | XP_013446424.1 | 86.7 | 490 | 55 | 4 | 1 | 480 | 1 | 490 | 1.80E-200 | 708.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44986.t1 | O64827 | 26.7 | 517 | 266 | 11 | 43 | 448 | 409 | 923 | 8.6e-34 | 146.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44986.t1 | A0A072TTG4 | 86.7 | 490 | 55 | 4 | 1 | 480 | 1 | 490 | 1.3e-200 | 708.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene44986.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049254 | MS.gene44986 | 0.808721 | 2.69E-50 | -1.69E-46 |
| MS.gene049272 | MS.gene44986 | 0.821053 | 4.95E-53 | -1.69E-46 |
| MS.gene049378 | MS.gene44986 | 0.805025 | 1.63E-49 | -1.69E-46 |
| MS.gene049567 | MS.gene44986 | 0.806877 | 6.64E-50 | -1.69E-46 |
| MS.gene049669 | MS.gene44986 | 0.800241 | 1.58E-48 | -1.69E-46 |
| MS.gene049825 | MS.gene44986 | 0.805525 | 1.28E-49 | -1.69E-46 |
| MS.gene049873 | MS.gene44986 | 0.815124 | 1.09E-51 | -1.69E-46 |
| MS.gene050026 | MS.gene44986 | 0.806301 | 8.79E-50 | -1.69E-46 |
| MS.gene050156 | MS.gene44986 | 0.831515 | 1.60E-55 | -1.69E-46 |
| MS.gene050201 | MS.gene44986 | 0.824421 | 8.14E-54 | -1.69E-46 |
| MS.gene050227 | MS.gene44986 | 0.813856 | 2.07E-51 | -1.69E-46 |
| MS.gene050228 | MS.gene44986 | 0.826315 | 2.90E-54 | -1.69E-46 |
| MS.gene050303 | MS.gene44986 | 0.812219 | 4.73E-51 | -1.69E-46 |
| MS.gene050355 | MS.gene44986 | 0.834416 | 3.04E-56 | -1.69E-46 |
| MS.gene050442 | MS.gene44986 | 0.81503 | 1.14E-51 | -1.69E-46 |
| MS.gene050719 | MS.gene44986 | 0.86224 | 5.83E-64 | -1.69E-46 |
| MS.gene050721 | MS.gene44986 | 0.86145 | 1.02E-63 | -1.69E-46 |
| MS.gene050724 | MS.gene44986 | 0.828805 | 7.33E-55 | -1.69E-46 |
| MS.gene050780 | MS.gene44986 | 0.842849 | 2.01E-58 | -1.69E-46 |
| MS.gene050799 | MS.gene44986 | 0.815345 | 9.70E-52 | -1.69E-46 |
| MS.gene050824 | MS.gene44986 | 0.815535 | 8.80E-52 | -1.69E-46 |
| MS.gene050844 | MS.gene44986 | 0.818698 | 1.71E-52 | -1.69E-46 |
| MS.gene050953 | MS.gene44986 | 0.8031 | 4.09E-49 | -1.69E-46 |
| MS.gene051207 | MS.gene44986 | 0.819761 | 9.80E-53 | -1.69E-46 |
| MS.gene051247 | MS.gene44986 | 0.816059 | 6.72E-52 | -1.69E-46 |
| MS.gene051265 | MS.gene44986 | 0.807142 | 5.84E-50 | -1.69E-46 |
| MS.gene051744 | MS.gene44986 | 0.802862 | 4.59E-49 | -1.69E-46 |
| MS.gene051935 | MS.gene44986 | 0.811532 | 6.68E-51 | -1.69E-46 |
| MS.gene052012 | MS.gene44986 | 0.804235 | 2.38E-49 | -1.69E-46 |
| MS.gene052176 | MS.gene44986 | 0.816641 | 4.98E-52 | -1.69E-46 |
| MS.gene052190 | MS.gene44986 | 0.845326 | 4.35E-59 | -1.69E-46 |
| MS.gene052208 | MS.gene44986 | 0.823099 | 1.66E-53 | -1.69E-46 |
| MS.gene052333 | MS.gene44986 | 0.822751 | 2.00E-53 | -1.69E-46 |
| MS.gene052358 | MS.gene44986 | -0.842889 | 1.96E-58 | -1.69E-46 |
| MS.gene052384 | MS.gene44986 | 0.802424 | 5.65E-49 | -1.69E-46 |
| MS.gene052519 | MS.gene44986 | 0.831042 | 2.09E-55 | -1.69E-46 |
| MS.gene052582 | MS.gene44986 | 0.804591 | 2.01E-49 | -1.69E-46 |
| MS.gene052584 | MS.gene44986 | 0.813279 | 2.77E-51 | -1.69E-46 |
| MS.gene052586 | MS.gene44986 | 0.83495 | 2.23E-56 | -1.69E-46 |
| MS.gene052773 | MS.gene44986 | 0.819946 | 8.89E-53 | -1.69E-46 |
| MS.gene052863 | MS.gene44986 | 0.819245 | 1.28E-52 | -1.69E-46 |
| MS.gene052896 | MS.gene44986 | 0.840523 | 8.26E-58 | -1.69E-46 |
| MS.gene053006 | MS.gene44986 | 0.819184 | 1.33E-52 | -1.69E-46 |
| MS.gene053054 | MS.gene44986 | -0.812495 | 4.12E-51 | -1.69E-46 |
| MS.gene053221 | MS.gene44986 | 0.825326 | 4.98E-54 | -1.69E-46 |
| MS.gene053226 | MS.gene44986 | 0.807325 | 5.34E-50 | -1.69E-46 |
| MS.gene053294 | MS.gene44986 | 0.800165 | 1.64E-48 | -1.69E-46 |
| MS.gene05365 | MS.gene44986 | 0.805069 | 1.59E-49 | -1.69E-46 |
| MS.gene053953 | MS.gene44986 | 0.802461 | 5.55E-49 | -1.69E-46 |
| MS.gene053966 | MS.gene44986 | 0.824173 | 9.32E-54 | -1.69E-46 |
| MS.gene05414 | MS.gene44986 | 0.826182 | 3.12E-54 | -1.69E-46 |
| MS.gene05415 | MS.gene44986 | 0.827881 | 1.22E-54 | -1.69E-46 |
| MS.gene054160 | MS.gene44986 | 0.80147 | 8.87E-49 | -1.69E-46 |
| MS.gene054291 | MS.gene44986 | -0.826504 | 2.62E-54 | -1.69E-46 |
| MS.gene054467 | MS.gene44986 | 0.811821 | 5.78E-51 | -1.69E-46 |
| MS.gene054468 | MS.gene44986 | 0.851264 | 9.94E-61 | -1.69E-46 |
| MS.gene054658 | MS.gene44986 | 0.803276 | 3.77E-49 | -1.69E-46 |
| MS.gene055357 | MS.gene44986 | 0.80197 | 7.00E-49 | -1.69E-46 |
| MS.gene055358 | MS.gene44986 | 0.809015 | 2.33E-50 | -1.69E-46 |
| MS.gene055586 | MS.gene44986 | 0.80111 | 1.05E-48 | -1.69E-46 |
| MS.gene055701 | MS.gene44986 | 0.800629 | 1.32E-48 | -1.69E-46 |
| MS.gene055778 | MS.gene44986 | 0.834802 | 2.43E-56 | -1.69E-46 |
| MS.gene055818 | MS.gene44986 | 0.847496 | 1.11E-59 | -1.69E-46 |
| MS.gene056091 | MS.gene44986 | 0.812741 | 3.64E-51 | -1.69E-46 |
| MS.gene056373 | MS.gene44986 | 0.822787 | 1.96E-53 | -1.69E-46 |
| MS.gene056506 | MS.gene44986 | 0.832419 | 9.55E-56 | -1.69E-46 |
| MS.gene05690 | MS.gene44986 | 0.801022 | 1.10E-48 | -1.69E-46 |
| MS.gene057026 | MS.gene44986 | 0.858123 | 1.02E-62 | -1.69E-46 |
| MS.gene057027 | MS.gene44986 | 0.800421 | 1.45E-48 | -1.69E-46 |
| MS.gene057060 | MS.gene44986 | 0.807462 | 4.99E-50 | -1.69E-46 |
| MS.gene057314 | MS.gene44986 | 0.805864 | 1.09E-49 | -1.69E-46 |
| MS.gene057693 | MS.gene44986 | 0.801138 | 1.04E-48 | -1.69E-46 |
| MS.gene057915 | MS.gene44986 | 0.813245 | 2.82E-51 | -1.69E-46 |
| MS.gene05795 | MS.gene44986 | 0.800133 | 1.66E-48 | -1.69E-46 |
| MS.gene057980 | MS.gene44986 | 0.806915 | 6.52E-50 | -1.69E-46 |
| MS.gene058037 | MS.gene44986 | 0.832572 | 8.76E-56 | -1.69E-46 |
| MS.gene058426 | MS.gene44986 | 0.80404 | 2.61E-49 | -1.69E-46 |
| MS.gene058625 | MS.gene44986 | 0.831038 | 2.09E-55 | -1.69E-46 |
| MS.gene058636 | MS.gene44986 | 0.831091 | 2.03E-55 | -1.69E-46 |
| MS.gene058829 | MS.gene44986 | 0.827892 | 1.22E-54 | -1.69E-46 |
| MS.gene058979 | MS.gene44986 | 0.8218 | 3.33E-53 | -1.69E-46 |
| MS.gene059249 | MS.gene44986 | 0.809251 | 2.08E-50 | -1.69E-46 |
| MS.gene059272 | MS.gene44986 | 0.803405 | 3.54E-49 | -1.69E-46 |
| MS.gene05954 | MS.gene44986 | 0.830284 | 3.20E-55 | -1.69E-46 |
| MS.gene059789 | MS.gene44986 | 0.84739 | 1.19E-59 | -1.69E-46 |
| MS.gene060322 | MS.gene44986 | 0.836215 | 1.07E-56 | -1.69E-46 |
| MS.gene060521 | MS.gene44986 | 0.812818 | 3.50E-51 | -1.69E-46 |
| MS.gene060553 | MS.gene44986 | 0.812744 | 3.63E-51 | -1.69E-46 |
| MS.gene060572 | MS.gene44986 | 0.864792 | 9.42E-65 | -1.69E-46 |
| MS.gene060586 | MS.gene44986 | 0.803118 | 4.06E-49 | -1.69E-46 |
| MS.gene060634 | MS.gene44986 | -0.806341 | 8.62E-50 | -1.69E-46 |
| MS.gene060683 | MS.gene44986 | 0.811075 | 8.39E-51 | -1.69E-46 |
| MS.gene060685 | MS.gene44986 | 0.813859 | 2.07E-51 | -1.69E-46 |
| MS.gene060781 | MS.gene44986 | 0.818523 | 1.87E-52 | -1.69E-46 |
| MS.gene060879 | MS.gene44986 | 0.808171 | 3.53E-50 | -1.69E-46 |
| MS.gene061040 | MS.gene44986 | -0.872021 | 4.38E-67 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44986.t1 | MTR_8g078700 | 86.939 | 490 | 54 | 4 | 1 | 480 | 1 | 490 | 0.0 | 866 |
| MS.gene44986.t1 | MTR_8g078490 | 62.039 | 461 | 148 | 7 | 33 | 475 | 387 | 838 | 0.0 | 546 |
| MS.gene44986.t1 | MTR_4g132610 | 58.917 | 314 | 104 | 3 | 39 | 345 | 367 | 662 | 6.16e-113 | 358 |
| MS.gene44986.t1 | MTR_5g018850 | 58.647 | 266 | 99 | 5 | 218 | 475 | 817 | 1079 | 3.77e-92 | 306 |
| MS.gene44986.t1 | MTR_5g018850 | 41.781 | 146 | 78 | 1 | 56 | 194 | 507 | 652 | 5.12e-27 | 115 |
| MS.gene44986.t1 | MTR_8g078505 | 59.350 | 246 | 52 | 2 | 20 | 228 | 6 | 240 | 2.42e-86 | 275 |
| MS.gene44986.t1 | MTR_4g132670 | 41.870 | 246 | 75 | 4 | 78 | 322 | 210 | 388 | 6.42e-49 | 173 |
| MS.gene44986.t1 | MTR_4g132670 | 56.897 | 58 | 25 | 0 | 58 | 115 | 55 | 112 | 6.40e-14 | 73.9 |
| MS.gene44986.t1 | MTR_8g442480 | 50.562 | 89 | 41 | 2 | 63 | 148 | 2 | 90 | 4.10e-19 | 82.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44986.t1 | AT2G23740 | 49.746 | 197 | 97 | 2 | 252 | 448 | 722 | 916 | 3.97e-54 | 196 |
| MS.gene44986.t1 | AT2G23740 | 40.602 | 133 | 71 | 2 | 45 | 169 | 404 | 536 | 3.94e-20 | 94.7 |
| MS.gene44986.t1 | AT2G23740 | 49.746 | 197 | 97 | 2 | 252 | 448 | 729 | 923 | 4.72e-54 | 196 |
| MS.gene44986.t1 | AT2G23740 | 40.602 | 133 | 71 | 2 | 45 | 169 | 411 | 543 | 4.12e-20 | 94.7 |
| MS.gene44986.t1 | AT2G23740 | 49.746 | 197 | 97 | 2 | 252 | 448 | 729 | 923 | 4.72e-54 | 196 |
| MS.gene44986.t1 | AT2G23740 | 40.602 | 133 | 71 | 2 | 45 | 169 | 411 | 543 | 4.12e-20 | 94.7 |
| MS.gene44986.t1 | AT2G23740 | 49.746 | 197 | 97 | 2 | 252 | 448 | 729 | 923 | 4.72e-54 | 196 |
| MS.gene44986.t1 | AT2G23740 | 40.602 | 133 | 71 | 2 | 45 | 169 | 411 | 543 | 4.12e-20 | 94.7 |
| MS.gene44986.t1 | AT2G23740 | 49.746 | 197 | 97 | 2 | 252 | 448 | 729 | 923 | 4.79e-54 | 196 |
| MS.gene44986.t1 | AT2G23740 | 40.602 | 133 | 71 | 2 | 45 | 169 | 411 | 543 | 3.65e-20 | 94.7 |
| MS.gene44986.t1 | AT2G23740 | 49.746 | 197 | 97 | 2 | 252 | 448 | 729 | 923 | 4.79e-54 | 196 |
| MS.gene44986.t1 | AT2G23740 | 40.602 | 133 | 71 | 2 | 45 | 169 | 411 | 543 | 3.65e-20 | 94.7 |
Find 94 sgRNAs with CRISPR-Local
Find 108 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCCTTCAGATAGAGCCTTTC+TGG | 0.146763 | 8.1:-28888577 | None:intergenic |
| TCCAAACCTCTTTCAAATTT+AGG | 0.154205 | 8.1:-28888390 | None:intergenic |
| GGCGAAACGCTCAAGCTTTC+CGG | 0.154625 | 8.1:+28887712 | MS.gene44986:CDS |
| ATTCCTTGTGGTTGTCATTT+TGG | 0.199979 | 8.1:+28888024 | MS.gene44986:CDS |
| TTGATCTCCAATGGACTTTC+CGG | 0.248032 | 8.1:-28887637 | None:intergenic |
| CCAGCCTCATGCAGATAAAT+TGG | 0.273277 | 8.1:+28888506 | MS.gene44986:CDS |
| TTGGAAATATAGAGGAATTA+TGG | 0.274132 | 8.1:+28888043 | MS.gene44986:CDS |
| GGCAAATAAGTTGCTTGAAA+TGG | 0.279485 | 8.1:+28888470 | MS.gene44986:CDS |
| CCTCTTTCAAATTTAGGATT+AGG | 0.281407 | 8.1:-28888384 | None:intergenic |
| TTCTTCGCAAAGAACATCTT+TGG | 0.285228 | 8.1:+28887691 | MS.gene44986:CDS |
| ACGCAAATATGTTCTTCATT+AGG | 0.299340 | 8.1:-28887196 | None:intergenic |
| CTTGACATTGTCGACCCTTT+TGG | 0.303194 | 8.1:-28887303 | None:intergenic |
| TCCTAAATTTGAAAGAGGTT+TGG | 0.365758 | 8.1:+28888389 | MS.gene44986:CDS |
| CCATCATCAAGCTGCTCATA+TGG | 0.373746 | 8.1:+28888278 | MS.gene44986:CDS |
| GCCTGTCCACTGATCATTAC+CGG | 0.388651 | 8.1:-28887731 | None:intergenic |
| GCAGTTGTACCTCCACAAAA+TGG | 0.390067 | 8.1:-28887436 | None:intergenic |
| TCCAATGGTTGCCAAGTGTT+TGG | 0.392168 | 8.1:-28887849 | None:intergenic |
| TGTACGAGATATTAAATTTG+AGG | 0.392916 | 8.1:-28887542 | None:intergenic |
| GCAAATAAGTTGCTTGAAAT+GGG | 0.399729 | 8.1:+28888471 | MS.gene44986:CDS |
| AGTCTTTGCAAATCAGGCTC+TGG | 0.401375 | 8.1:-28887600 | None:intergenic |
| CGATCTAGCCAGCAGGCGCT+TGG | 0.413605 | 8.1:+28888305 | MS.gene44986:CDS |
| ACACCAAGTCTTTGCAAATC+AGG | 0.417723 | 8.1:-28887606 | None:intergenic |
| GGAAGAATCTCAATGCTTAG+AGG | 0.420258 | 8.1:+28888527 | MS.gene44986:CDS |
| AGTTATCGAAAGCTTAAATT+CGG | 0.424504 | 8.1:-28888091 | None:intergenic |
| CCTAATCCTAAATTTGAAAG+AGG | 0.427935 | 8.1:+28888384 | MS.gene44986:CDS |
| GTTTGGAAGAAGCATCAAAT+AGG | 0.428853 | 8.1:+28888406 | MS.gene44986:CDS |
| AGACTTGGTGTTTGTACATC+CGG | 0.429428 | 8.1:+28887618 | MS.gene44986:CDS |
| ATCGAAAGCTTAAATTCGGC+AGG | 0.430592 | 8.1:-28888087 | None:intergenic |
| TTTCATGACTTTCCATCCAA+TGG | 0.430775 | 8.1:-28887864 | None:intergenic |
| CCAATTTATCTGCATGAGGC+TGG | 0.431124 | 8.1:-28888506 | None:intergenic |
| AAGAGACACTATGGGCCGTT+TGG | 0.440817 | 8.1:+28887976 | MS.gene44986:CDS |
| ACTCTTTGCCAAGCGCCTGC+TGG | 0.443750 | 8.1:-28888313 | None:intergenic |
| CAGTGACAGTTCATCTGTAA+TGG | 0.451819 | 8.1:+28887261 | MS.gene44986:CDS |
| GTTCGCGGAAGTTTAGTTGC+AGG | 0.454226 | 8.1:+28888214 | MS.gene44986:CDS |
| GAGATATTAAATTTGAGGTC+TGG | 0.457795 | 8.1:-28887537 | None:intergenic |
| TAATTTCAAGAATAGAAATC+AGG | 0.462085 | 8.1:+28888449 | MS.gene44986:CDS |
| TTCTCCTAAAATGATTGAGC+AGG | 0.465200 | 8.1:+28888146 | MS.gene44986:CDS |
| TGATGATGGCGACCGAGATC+AGG | 0.469915 | 8.1:-28888264 | None:intergenic |
| GTCTTTGCAAATCAGGCTCT+GGG | 0.470035 | 8.1:-28887599 | None:intergenic |
| TAATATCTCGTACATTAAAG+AGG | 0.473802 | 8.1:+28887551 | MS.gene44986:CDS |
| ACCAAACACTTGGCAACCAT+TGG | 0.477256 | 8.1:+28887848 | MS.gene44986:CDS |
| GACTCTTATAGAAAACCAAA+AGG | 0.481717 | 8.1:+28887288 | MS.gene44986:CDS |
| CCATATGAGCAGCTTGATGA+TGG | 0.488631 | 8.1:-28888278 | None:intergenic |
| TGAGAGTGCTGTTAAGTGTA+AGG | 0.490329 | 8.1:+28887801 | MS.gene44986:CDS |
| TTGGATGGAAAGTCATGAAA+AGG | 0.491304 | 8.1:+28887867 | MS.gene44986:CDS |
| TCATCATTGACGATTGACTC+TGG | 0.492547 | 8.1:-28887661 | None:intergenic |
| AGTGAAGGAATCAAAACAAA+AGG | 0.493161 | 8.1:-28887923 | None:intergenic |
| CATGTTCAGAAGAGACACTA+TGG | 0.495523 | 8.1:+28887967 | MS.gene44986:CDS |
| ACATCTGCACAAAGCTGAGA+TGG | 0.500567 | 8.1:+28887513 | MS.gene44986:CDS |
| AGCAAGCAGTGTTCACCAAA+CGG | 0.504892 | 8.1:-28887991 | None:intergenic |
| CAGTGCGTGAGAACCGCAAT+TGG | 0.508352 | 8.1:+28887349 | MS.gene44986:CDS |
| GTTTCGTTTATCTACCAGAA+AGG | 0.508712 | 8.1:+28888563 | MS.gene44986:CDS |
| TATGCTCAGAGACTAAAATC+TGG | 0.515386 | 8.1:+28888348 | MS.gene44986:CDS |
| GAGCCTGATTTGCAAAGACT+TGG | 0.521361 | 8.1:+28887603 | MS.gene44986:CDS |
| TTCAAATTTAGGATTAGGAC+GGG | 0.522702 | 8.1:-28888379 | None:intergenic |
| ATTAGCTTTAGCCCTTCTCC+TGG | 0.523241 | 8.1:-28888430 | None:intergenic |
| TCCGGTAATGATCAGTGGAC+AGG | 0.523358 | 8.1:+28887730 | MS.gene44986:CDS |
| TGGAGCGCGATCTAGCCAGC+AGG | 0.523628 | 8.1:+28888298 | MS.gene44986:CDS |
| AGCAGGCGCTTGGCAAAGAG+TGG | 0.523735 | 8.1:+28888315 | MS.gene44986:CDS |
| ACAAATCAAGGAGCAGCTTA+CGG | 0.524363 | 8.1:+28887172 | MS.gene44986:CDS |
| GACTCTGGATTGATCTCCAA+TGG | 0.529884 | 8.1:-28887646 | None:intergenic |
| CTGAGAATCGAAGTGGTTCG+CGG | 0.530378 | 8.1:+28888199 | MS.gene44986:CDS |
| GTGTATATTGTTACCAATTG+CGG | 0.530995 | 8.1:-28887362 | None:intergenic |
| TGTACATCCGGAAAGTCCAT+TGG | 0.533385 | 8.1:+28887630 | MS.gene44986:CDS |
| CATCAAATAGGACCAGGAGA+AGG | 0.534319 | 8.1:+28888418 | MS.gene44986:CDS |
| TTTGATTTACTACCTGATCT+CGG | 0.536142 | 8.1:+28888252 | MS.gene44986:CDS |
| AACACTTGGCAACCATTGGA+TGG | 0.536290 | 8.1:+28887852 | MS.gene44986:CDS |
| TTTCAAATTTAGGATTAGGA+CGG | 0.543514 | 8.1:-28888380 | None:intergenic |
| ATCAAATAGGACCAGGAGAA+GGG | 0.546017 | 8.1:+28888419 | MS.gene44986:CDS |
| TTTATCTGCATGAGGCTGGA+TGG | 0.546716 | 8.1:-28888502 | None:intergenic |
| TCTTCCAATTTATCTGCATG+AGG | 0.563398 | 8.1:-28888510 | None:intergenic |
| GATTGCTCTGAGACAAATCA+AGG | 0.567116 | 8.1:+28887160 | MS.gene44986:CDS |
| GACAGTTCATCTGTAATGGA+AGG | 0.570153 | 8.1:+28887265 | MS.gene44986:CDS |
| ACCAGAAAGGCTCTATCTGA+AGG | 0.579442 | 8.1:+28888576 | MS.gene44986:CDS |
| CATCTGCACAAAGCTGAGAT+GGG | 0.579947 | 8.1:+28887514 | MS.gene44986:CDS |
| ACTCTTATAGAAAACCAAAA+GGG | 0.580021 | 8.1:+28887289 | MS.gene44986:CDS |
| TGGACAGGCTCTCCTAGCTA+TGG | 0.583120 | 8.1:+28887745 | MS.gene44986:CDS |
| TTATGATCATTACCATAGCT+AGG | 0.583706 | 8.1:-28887757 | None:intergenic |
| TGTGGAGGTACAACTGCTGC+TGG | 0.586893 | 8.1:+28887442 | MS.gene44986:CDS |
| TCTCCTAAAATGATTGAGCA+GGG | 0.608885 | 8.1:+28888147 | MS.gene44986:CDS |
| GAAGCACAGTGGTTGTTCAG+AGG | 0.608970 | 8.1:+28887889 | MS.gene44986:CDS |
| GATAATTCTGAGAATCGAAG+TGG | 0.614192 | 8.1:+28888192 | MS.gene44986:CDS |
| AGCTTTCCGGTAATGATCAG+TGG | 0.616263 | 8.1:+28887725 | MS.gene44986:CDS |
| CATATATCGAAGACAAGGGA+AGG | 0.617551 | 8.1:+28887326 | MS.gene44986:CDS |
| GAGCAACTAACATTGTCTAC+CGG | 0.626990 | 8.1:+28888117 | MS.gene44986:CDS |
| TCAAGCATATATCGAAGACA+AGG | 0.630094 | 8.1:+28887321 | MS.gene44986:CDS |
| ACAGTTCATCTGTAATGGAA+GGG | 0.632090 | 8.1:+28887266 | MS.gene44986:CDS |
| AAGAAGCATCAAATAGGACC+AGG | 0.634670 | 8.1:+28888412 | MS.gene44986:CDS |
| ATGTTCAGAAGAGACACTAT+GGG | 0.654163 | 8.1:+28887968 | MS.gene44986:CDS |
| TCGAAAGCTTAAATTCGGCA+GGG | 0.657128 | 8.1:-28888086 | None:intergenic |
| AAAGCTGAACAAAATTGCAG+TGG | 0.668916 | 8.1:+28887574 | MS.gene44986:CDS |
| CAAGCATATATCGAAGACAA+GGG | 0.692685 | 8.1:+28887322 | MS.gene44986:CDS |
| TTTCCAAAATGACAACCACA+AGG | 0.715599 | 8.1:-28888027 | None:intergenic |
| GTCATGAAAAGGAAGCACAG+TGG | 0.767507 | 8.1:+28887878 | MS.gene44986:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTTCTTAAGTAAAAAAGAA+AGG | + | chr8.1:28887395-28887414 | MS.gene44986:CDS | 15.0% |
| !! | TAATTTCAAGAATAGAAATC+AGG | + | chr8.1:28888449-28888468 | MS.gene44986:CDS | 20.0% |
| !! | TTCTTACAAAAAGATAATCT+GGG | - | chr8.1:28887493-28887512 | None:intergenic | 20.0% |
| !! | TTTCTTACAAAAAGATAATC+TGG | - | chr8.1:28887494-28887513 | None:intergenic | 20.0% |
| ! | ACTCTTATAGAAAACCAAAA+GGG | + | chr8.1:28887289-28887308 | MS.gene44986:CDS | 25.0% |
| ! | AGTTATCGAAAGCTTAAATT+CGG | - | chr8.1:28888094-28888113 | None:intergenic | 25.0% |
| ! | CTTCACTAACAAAAAACTAT+TGG | + | chr8.1:28887939-28887958 | MS.gene44986:CDS | 25.0% |
| ! | TAATATCTCGTACATTAAAG+AGG | + | chr8.1:28887551-28887570 | MS.gene44986:CDS | 25.0% |
| ! | TGTACGAGATATTAAATTTG+AGG | - | chr8.1:28887545-28887564 | None:intergenic | 25.0% |
| ! | TTGGAAATATAGAGGAATTA+TGG | + | chr8.1:28888043-28888062 | MS.gene44986:CDS | 25.0% |
| !! | TTTCAAATTTAGGATTAGGA+CGG | - | chr8.1:28888383-28888402 | None:intergenic | 25.0% |
| !!! | CAATAGTTTTTTGTTAGTGA+AGG | - | chr8.1:28887941-28887960 | None:intergenic | 25.0% |
| !!! | TTGTCATTTTGGAAATATAG+AGG | + | chr8.1:28888035-28888054 | MS.gene44986:CDS | 25.0% |
| ACAAAAAGATAATCTGGGAA+GGG | - | chr8.1:28887488-28887507 | None:intergenic | 30.0% | |
| ACGCAAATATGTTCTTCATT+AGG | - | chr8.1:28887199-28887218 | None:intergenic | 30.0% | |
| AGTGAAGGAATCAAAACAAA+AGG | - | chr8.1:28887926-28887945 | None:intergenic | 30.0% | |
| CCTAATCCTAAATTTGAAAG+AGG | + | chr8.1:28888384-28888403 | MS.gene44986:CDS | 30.0% | |
| GACTCTTATAGAAAACCAAA+AGG | + | chr8.1:28887288-28887307 | MS.gene44986:CDS | 30.0% | |
| GCAAATAAGTTGCTTGAAAT+GGG | + | chr8.1:28888471-28888490 | MS.gene44986:CDS | 30.0% | |
| GTGTATATTGTTACCAATTG+CGG | - | chr8.1:28887365-28887384 | None:intergenic | 30.0% | |
| TACAAAAAGATAATCTGGGA+AGG | - | chr8.1:28887489-28887508 | None:intergenic | 30.0% | |
| TCCAAACCTCTTTCAAATTT+AGG | - | chr8.1:28888393-28888412 | None:intergenic | 30.0% | |
| TCCTAAATTTGAAAGAGGTT+TGG | + | chr8.1:28888389-28888408 | MS.gene44986:CDS | 30.0% | |
| TTATGATCATTACCATAGCT+AGG | - | chr8.1:28887760-28887779 | None:intergenic | 30.0% | |
| ! | CCTCTTTCAAATTTAGGATT+AGG | - | chr8.1:28888387-28888406 | None:intergenic | 30.0% |
| ! | CTTTTTCTATGTATTCCTTG+TGG | + | chr8.1:28888012-28888031 | MS.gene44986:CDS | 30.0% |
| ! | GAGATATTAAATTTGAGGTC+TGG | - | chr8.1:28887540-28887559 | None:intergenic | 30.0% |
| ! | TTCAAATTTAGGATTAGGAC+GGG | - | chr8.1:28888382-28888401 | None:intergenic | 30.0% |
| ! | TTTGATTTACTACCTGATCT+CGG | + | chr8.1:28888252-28888271 | MS.gene44986:CDS | 30.0% |
| ! | TTTTCTGATAACCAAACACT+TGG | + | chr8.1:28887838-28887857 | MS.gene44986:CDS | 30.0% |
| AAAGCTGAACAAAATTGCAG+TGG | + | chr8.1:28887574-28887593 | MS.gene44986:CDS | 35.0% | |
| ATGTTCAGAAGAGACACTAT+GGG | + | chr8.1:28887968-28887987 | MS.gene44986:CDS | 35.0% | |
| CAAGCATATATCGAAGACAA+GGG | + | chr8.1:28887322-28887341 | MS.gene44986:CDS | 35.0% | |
| GGCAAATAAGTTGCTTGAAA+TGG | + | chr8.1:28888470-28888489 | MS.gene44986:CDS | 35.0% | |
| GTTTCGTTTATCTACCAGAA+AGG | + | chr8.1:28888563-28888582 | MS.gene44986:CDS | 35.0% | |
| TATGCTCAGAGACTAAAATC+TGG | + | chr8.1:28888348-28888367 | MS.gene44986:CDS | 35.0% | |
| TCAAGCATATATCGAAGACA+AGG | + | chr8.1:28887321-28887340 | MS.gene44986:CDS | 35.0% | |
| TCTCCTAAAATGATTGAGCA+GGG | + | chr8.1:28888147-28888166 | MS.gene44986:CDS | 35.0% | |
| TCTTCCAATTTATCTGCATG+AGG | - | chr8.1:28888513-28888532 | None:intergenic | 35.0% | |
| TTCTCCTAAAATGATTGAGC+AGG | + | chr8.1:28888146-28888165 | MS.gene44986:CDS | 35.0% | |
| TTCTTCGCAAAGAACATCTT+TGG | + | chr8.1:28887691-28887710 | MS.gene44986:CDS | 35.0% | |
| TTGGATGGAAAGTCATGAAA+AGG | + | chr8.1:28887867-28887886 | MS.gene44986:CDS | 35.0% | |
| TTTCATGACTTTCCATCCAA+TGG | - | chr8.1:28887867-28887886 | None:intergenic | 35.0% | |
| TTTCCAAAATGACAACCACA+AGG | - | chr8.1:28888030-28888049 | None:intergenic | 35.0% | |
| ! | AAAGCAATCACTCCATTTTG+TGG | + | chr8.1:28887424-28887443 | MS.gene44986:CDS | 35.0% |
| ! | ACAGTTCATCTGTAATGGAA+GGG | + | chr8.1:28887266-28887285 | MS.gene44986:CDS | 35.0% |
| ! | GATAATTCTGAGAATCGAAG+TGG | + | chr8.1:28888192-28888211 | MS.gene44986:CDS | 35.0% |
| ! | TTTCCCTGCTCAATCATTTT+AGG | - | chr8.1:28888153-28888172 | None:intergenic | 35.0% |
| !! | ATTCCTTGTGGTTGTCATTT+TGG | + | chr8.1:28888024-28888043 | MS.gene44986:CDS | 35.0% |
| !! | CATTTTAGGAGAATCATCAC+CGG | - | chr8.1:28888139-28888158 | None:intergenic | 35.0% |
| !! | GTTTGGAAGAAGCATCAAAT+AGG | + | chr8.1:28888406-28888425 | MS.gene44986:CDS | 35.0% |
| ACAAATCAAGGAGCAGCTTA+CGG | + | chr8.1:28887172-28887191 | MS.gene44986:CDS | 40.0% | |
| ACACCAAGTCTTTGCAAATC+AGG | - | chr8.1:28887609-28887628 | None:intergenic | 40.0% | |
| ATCAAATAGGACCAGGAGAA+GGG | + | chr8.1:28888419-28888438 | MS.gene44986:CDS | 40.0% | |
| ATCGAAAGCTTAAATTCGGC+AGG | - | chr8.1:28888090-28888109 | None:intergenic | 40.0% | |
| CAGTGACAGTTCATCTGTAA+TGG | + | chr8.1:28887261-28887280 | MS.gene44986:CDS | 40.0% | |
| CATATATCGAAGACAAGGGA+AGG | + | chr8.1:28887326-28887345 | MS.gene44986:CDS | 40.0% | |
| CATGTTCAGAAGAGACACTA+TGG | + | chr8.1:28887967-28887986 | MS.gene44986:CDS | 40.0% | |
| GAGCAACTAACATTGTCTAC+CGG | + | chr8.1:28888117-28888136 | MS.gene44986:CDS | 40.0% | |
| GGAAGAATCTCAATGCTTAG+AGG | + | chr8.1:28888527-28888546 | MS.gene44986:CDS | 40.0% | |
| TCGAAAGCTTAAATTCGGCA+GGG | - | chr8.1:28888089-28888108 | None:intergenic | 40.0% | |
| ! | GACAGTTCATCTGTAATGGA+AGG | + | chr8.1:28887265-28887284 | MS.gene44986:CDS | 40.0% |
| ! | TCATCATTGACGATTGACTC+TGG | - | chr8.1:28887664-28887683 | None:intergenic | 40.0% |
| ! | TTGATCTCCAATGGACTTTC+CGG | - | chr8.1:28887640-28887659 | None:intergenic | 40.0% |
| !! | AAGAAGCATCAAATAGGACC+AGG | + | chr8.1:28888412-28888431 | MS.gene44986:CDS | 40.0% |
| !! | AGACTTGGTGTTTGTACATC+CGG | + | chr8.1:28887618-28887637 | MS.gene44986:CDS | 40.0% |
| !! | GATTGCTCTGAGACAAATCA+AGG | + | chr8.1:28887160-28887179 | MS.gene44986:CDS | 40.0% |
| !! | TGAGAGTGCTGTTAAGTGTA+AGG | + | chr8.1:28887801-28887820 | MS.gene44986:CDS | 40.0% |
| AACACTTGGCAACCATTGGA+TGG | + | chr8.1:28887852-28887871 | MS.gene44986:CDS | 45.0% | |
| ACATCTGCACAAAGCTGAGA+TGG | + | chr8.1:28887513-28887532 | MS.gene44986:CDS | 45.0% | |
| ACCAAACACTTGGCAACCAT+TGG | + | chr8.1:28887848-28887867 | MS.gene44986:CDS | 45.0% | |
| ACCAGAAAGGCTCTATCTGA+AGG | + | chr8.1:28888576-28888595 | MS.gene44986:CDS | 45.0% | |
| AGCAAGCAGTGTTCACCAAA+CGG | - | chr8.1:28887994-28888013 | None:intergenic | 45.0% | |
| AGCTTTCCGGTAATGATCAG+TGG | + | chr8.1:28887725-28887744 | MS.gene44986:CDS | 45.0% | |
| AGTCTTTGCAAATCAGGCTC+TGG | - | chr8.1:28887603-28887622 | None:intergenic | 45.0% | |
| CATCAAATAGGACCAGGAGA+AGG | + | chr8.1:28888418-28888437 | MS.gene44986:CDS | 45.0% | |
| CATCTGCACAAAGCTGAGAT+GGG | + | chr8.1:28887514-28887533 | MS.gene44986:CDS | 45.0% | |
| CCAATTTATCTGCATGAGGC+TGG | - | chr8.1:28888509-28888528 | None:intergenic | 45.0% | |
| CCAGCCTCATGCAGATAAAT+TGG | + | chr8.1:28888506-28888525 | MS.gene44986:CDS | 45.0% | |
| CCATATGAGCAGCTTGATGA+TGG | - | chr8.1:28888281-28888300 | None:intergenic | 45.0% | |
| CCATCATCAAGCTGCTCATA+TGG | + | chr8.1:28888278-28888297 | MS.gene44986:CDS | 45.0% | |
| GACTCTGGATTGATCTCCAA+TGG | - | chr8.1:28887649-28887668 | None:intergenic | 45.0% | |
| GCAGTTGTACCTCCACAAAA+TGG | - | chr8.1:28887439-28887458 | None:intergenic | 45.0% | |
| GTCTTTGCAAATCAGGCTCT+GGG | - | chr8.1:28887602-28887621 | None:intergenic | 45.0% | |
| TCCAATGGTTGCCAAGTGTT+TGG | - | chr8.1:28887852-28887871 | None:intergenic | 45.0% | |
| TGTACATCCGGAAAGTCCAT+TGG | + | chr8.1:28887630-28887649 | MS.gene44986:CDS | 45.0% | |
| TTTATCTGCATGAGGCTGGA+TGG | - | chr8.1:28888505-28888524 | None:intergenic | 45.0% | |
| ! | CTTGACATTGTCGACCCTTT+TGG | - | chr8.1:28887306-28887325 | None:intergenic | 45.0% |
| ! | GAGCCTGATTTGCAAAGACT+TGG | + | chr8.1:28887603-28887622 | MS.gene44986:CDS | 45.0% |
| ! | GCAATCACTCCATTTTGTGG+AGG | + | chr8.1:28887427-28887446 | MS.gene44986:CDS | 45.0% |
| ! | GTCATGAAAAGGAAGCACAG+TGG | + | chr8.1:28887878-28887897 | MS.gene44986:CDS | 45.0% |
| !! | ATTAGCTTTAGCCCTTCTCC+TGG | - | chr8.1:28888433-28888452 | None:intergenic | 45.0% |
| AAGAGACACTATGGGCCGTT+TGG | + | chr8.1:28887976-28887995 | MS.gene44986:CDS | 50.0% | |
| CTGAGAATCGAAGTGGTTCG+CGG | + | chr8.1:28888199-28888218 | MS.gene44986:CDS | 50.0% | |
| GAAGCACAGTGGTTGTTCAG+AGG | + | chr8.1:28887889-28887908 | MS.gene44986:CDS | 50.0% | |
| GCCTGTCCACTGATCATTAC+CGG | - | chr8.1:28887734-28887753 | None:intergenic | 50.0% | |
| GCCTTCAGATAGAGCCTTTC+TGG | - | chr8.1:28888580-28888599 | None:intergenic | 50.0% | |
| GTTCGCGGAAGTTTAGTTGC+AGG | + | chr8.1:28888214-28888233 | MS.gene44986:CDS | 50.0% | |
| TCCGGTAATGATCAGTGGAC+AGG | + | chr8.1:28887730-28887749 | MS.gene44986:CDS | 50.0% | |
| CAGTGCGTGAGAACCGCAAT+TGG | + | chr8.1:28887349-28887368 | MS.gene44986:CDS | 55.0% | |
| GGCGAAACGCTCAAGCTTTC+CGG | + | chr8.1:28887712-28887731 | MS.gene44986:CDS | 55.0% | |
| TGGACAGGCTCTCCTAGCTA+TGG | + | chr8.1:28887745-28887764 | MS.gene44986:CDS | 55.0% | |
| ! | TGTGGAGGTACAACTGCTGC+TGG | + | chr8.1:28887442-28887461 | MS.gene44986:CDS | 55.0% |
| !! | TGATGATGGCGACCGAGATC+AGG | - | chr8.1:28888267-28888286 | None:intergenic | 55.0% |
| ACTCTTTGCCAAGCGCCTGC+TGG | - | chr8.1:28888316-28888335 | None:intergenic | 60.0% | |
| AGCAGGCGCTTGGCAAAGAG+TGG | + | chr8.1:28888315-28888334 | MS.gene44986:CDS | 60.0% | |
| CGATCTAGCCAGCAGGCGCT+TGG | + | chr8.1:28888305-28888324 | MS.gene44986:CDS | 65.0% | |
| TGGAGCGCGATCTAGCCAGC+AGG | + | chr8.1:28888298-28888317 | MS.gene44986:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 28887142 | 28888617 | 28887142 | ID=MS.gene44986 |
| chr8.1 | mRNA | 28887142 | 28888617 | 28887142 | ID=MS.gene44986.t1;Parent=MS.gene44986 |
| chr8.1 | exon | 28887142 | 28888617 | 28887142 | ID=MS.gene44986.t1.exon1;Parent=MS.gene44986.t1 |
| chr8.1 | CDS | 28887142 | 28888617 | 28887142 | ID=cds.MS.gene44986.t1;Parent=MS.gene44986.t1 |
| Gene Sequence |
| Protein sequence |
