AlfalfaGEDB | MS.gene80770

M. sativa cultivar XinJiangDaYe / MS.gene80770

KEGG: -
GO: GO:0003674 GO:0005488 GO:0043167 GO:0043169 GO:0046872
PFAM: PF01439.21

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene80770.t1	XP_013455002.1	94.7	75	4	0	1	75	1	75	5.00E-25	123.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene80770.t1	P20830	92.0	75	6	0	1	75	1	75	6.6e-26	117.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene80770.t1	A0A072UT35	94.7	75	4	0	1	75	1	75	3.6e-25	123.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049378	MS.gene80770	0.824528	7.69E-54	-1.69E-46
MS.gene049452	MS.gene80770	0.812213	4.75E-51	-1.69E-46
MS.gene049669	MS.gene80770	0.807485	4.94E-50	-1.69E-46
MS.gene050026	MS.gene80770	0.807676	4.50E-50	-1.69E-46
MS.gene050105	MS.gene80770	0.827747	1.32E-54	-1.69E-46
MS.gene050227	MS.gene80770	0.805118	1.56E-49	-1.69E-46
MS.gene050228	MS.gene80770	0.815527	8.83E-52	-1.69E-46
MS.gene050680	MS.gene80770	0.854327	1.33E-61	-1.69E-46
MS.gene050750	MS.gene80770	0.838319	3.09E-57	-1.69E-46
MS.gene050953	MS.gene80770	0.842937	1.91E-58	-1.69E-46
MS.gene051368	MS.gene80770	0.84827	6.82E-60	-1.69E-46
MS.gene051924	MS.gene80770	-0.825083	5.69E-54	-1.69E-46
MS.gene051926	MS.gene80770	-0.82981	4.18E-55	-1.69E-46
MS.gene05216	MS.gene80770	0.819669	1.03E-52	-1.69E-46
MS.gene052176	MS.gene80770	0.818615	1.79E-52	-1.69E-46
MS.gene052190	MS.gene80770	0.862634	4.41E-64	-1.69E-46
MS.gene052333	MS.gene80770	0.863934	1.75E-64	-1.69E-46
MS.gene052389	MS.gene80770	0.805071	1.59E-49	-1.69E-46
MS.gene052519	MS.gene80770	0.800525	1.38E-48	-1.69E-46
MS.gene052586	MS.gene80770	0.847449	1.15E-59	-1.69E-46
MS.gene052674	MS.gene80770	0.816035	6.81E-52	-1.69E-46
MS.gene052675	MS.gene80770	0.81663	5.01E-52	-1.69E-46
MS.gene052677	MS.gene80770	0.820064	8.36E-53	-1.69E-46
MS.gene052867	MS.gene80770	0.806366	8.52E-50	-1.69E-46
MS.gene052954	MS.gene80770	0.837026	6.63E-57	-1.69E-46
MS.gene052965	MS.gene80770	-0.8237	1.20E-53	-1.69E-46
MS.gene053006	MS.gene80770	0.817584	3.06E-52	-1.69E-46
MS.gene053166	MS.gene80770	0.859945	2.91E-63	-1.69E-46
MS.gene053202	MS.gene80770	0.800665	1.30E-48	-1.69E-46
MS.gene05415	MS.gene80770	0.838006	3.71E-57	-1.69E-46
MS.gene054160	MS.gene80770	0.813379	2.64E-51	-1.69E-46
MS.gene054236	MS.gene80770	0.804028	2.63E-49	-1.69E-46
MS.gene054291	MS.gene80770	-0.851297	9.73E-61	-1.69E-46
MS.gene054405	MS.gene80770	0.839209	1.82E-57	-1.69E-46
MS.gene054468	MS.gene80770	0.815832	7.55E-52	-1.69E-46
MS.gene054479	MS.gene80770	0.82124	4.48E-53	-1.69E-46
MS.gene054559	MS.gene80770	0.84251	2.47E-58	-1.69E-46
MS.gene05510	MS.gene80770	0.816509	5.33E-52	-1.69E-46
MS.gene055227	MS.gene80770	0.825217	5.29E-54	-1.69E-46
MS.gene055228	MS.gene80770	0.809583	1.76E-50	-1.69E-46
MS.gene055230	MS.gene80770	0.813156	2.95E-51	-1.69E-46
MS.gene055356	MS.gene80770	0.812097	5.03E-51	-1.69E-46
MS.gene055367	MS.gene80770	0.811099	8.29E-51	-1.69E-46
MS.gene055701	MS.gene80770	0.888288	6.74E-73	-1.69E-46
MS.gene055818	MS.gene80770	0.816941	4.27E-52	-1.69E-46
MS.gene055841	MS.gene80770	0.81931	1.24E-52	-1.69E-46
MS.gene05595	MS.gene80770	0.819647	1.04E-52	-1.69E-46
MS.gene056173	MS.gene80770	0.832256	1.05E-55	-1.69E-46
MS.gene056366	MS.gene80770	0.800255	1.57E-48	-1.69E-46
MS.gene056480	MS.gene80770	0.810743	9.90E-51	-1.69E-46
MS.gene056506	MS.gene80770	0.847372	1.21E-59	-1.69E-46
MS.gene056779	MS.gene80770	0.802304	5.98E-49	-1.69E-46
MS.gene057026	MS.gene80770	0.819555	1.09E-52	-1.69E-46
MS.gene057060	MS.gene80770	0.813827	2.10E-51	-1.69E-46
MS.gene057156	MS.gene80770	-0.858675	7.00E-63	-1.69E-46
MS.gene057208	MS.gene80770	0.821296	4.35E-53	-1.69E-46
MS.gene057598	MS.gene80770	0.8065	7.98E-50	-1.69E-46
MS.gene05761	MS.gene80770	0.820229	7.66E-53	-1.69E-46
MS.gene058146	MS.gene80770	0.808916	2.45E-50	-1.69E-46
MS.gene058223	MS.gene80770	-0.805573	1.25E-49	-1.69E-46
MS.gene058595	MS.gene80770	0.809628	1.72E-50	-1.69E-46
MS.gene058979	MS.gene80770	0.830139	3.48E-55	-1.69E-46
MS.gene059128	MS.gene80770	0.810285	1.24E-50	-1.69E-46
MS.gene059382	MS.gene80770	0.818381	2.02E-52	-1.69E-46
MS.gene059584	MS.gene80770	0.806223	9.13E-50	-1.69E-46
MS.gene060011	MS.gene80770	0.839118	1.92E-57	-1.69E-46
MS.gene060310	MS.gene80770	0.822085	2.86E-53	-1.69E-46
MS.gene060322	MS.gene80770	0.818895	1.54E-52	-1.69E-46
MS.gene060572	MS.gene80770	0.813106	3.03E-51	-1.69E-46
MS.gene060634	MS.gene80770	-0.807621	4.62E-50	-1.69E-46
MS.gene060683	MS.gene80770	0.825985	3.48E-54	-1.69E-46
MS.gene060685	MS.gene80770	0.828764	7.50E-55	-1.69E-46
MS.gene060879	MS.gene80770	0.834905	2.29E-56	-1.69E-46
MS.gene060998	MS.gene80770	0.822121	2.81E-53	-1.69E-46
MS.gene061040	MS.gene80770	-0.827359	1.63E-54	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene80770.t1	MTR_4g021730	93.333	75	5	0	1	75	1	75	4.95e-45	139
MS.gene80770.t1	MTR_4g021725	82.667	75	13	0	1	75	1	75	5.74e-40	126
MS.gene80770.t1	MTR_7g061018	53.623	69	29	2	8	75	12	78	1.26e-12	57.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 21 sgRNAs with CRISPR-Local

Find 43 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGCACCTTCAAATTGGATTT+TGG	0.180739	4.4:-80133798	None:intergenic
AACCACAGAAACCGTGATTT+TGG	0.206196	4.4:+80133763	MS.gene80770:CDS
CCATTTCAGCACCTTCAAAT+TGG	0.262642	4.4:-80133805	None:intergenic
CCAATTTGAAGGTGCTGAAA+TGG	0.351001	4.4:+80133805	MS.gene80770:CDS
GGTTTCTGTGGTTTCACCTT+CGG	0.379271	4.4:-80133753	None:intergenic
GCACTTCATTTGCAGTTGCA+AGG	0.406733	4.4:-80133881	None:intergenic
CAATTTGAAGGTGCTGAAAT+GGG	0.457247	4.4:+80133806	MS.gene80770:CDS
CACTTCATTTGCAGTTGCAA+GGG	0.477731	4.4:-80133880	None:intergenic
GCCCAAAATCACGGTTTCTG+TGG	0.518698	4.4:-80133765	None:intergenic
CCAGCCAAAATCCAATTTGA+AGG	0.533386	4.4:+80133794	MS.gene80770:CDS
AATATGTCTAGCTGTGGTTG+TGG	0.535056	4.4:+80132802	None:intergenic
GAGGATGGTGGCTGCAAGTG+TGG	0.564753	4.4:+80133839	MS.gene80770:CDS
TGGACCGACGCCCAAAATCA+CGG	0.582883	4.4:-80133774	None:intergenic
AGATGCAACACGAGGTCTAG+TGG	0.601156	4.4:+80133719	MS.gene80770:intron
TGTGGAAGCAGTTGCAACTG+TGG	0.645986	4.4:+80132820	MS.gene80770:CDS
TCTGAAAATATGTCTAGCTG+TGG	0.657510	4.4:+80132796	None:intergenic
TACTTGGCAGATGCAACACG+AGG	0.684945	4.4:+80133711	MS.gene80770:intron
AGTGGATTGAGCTATGCCGA+AGG	0.688325	4.4:+80133737	MS.gene80770:CDS
ATGGGCGTTACATCTGAGGA+TGG	0.692405	4.4:+80133824	MS.gene80770:CDS
TGAAATGGGCGTTACATCTG+AGG	0.695359	4.4:+80133820	MS.gene80770:CDS
GGCGTTACATCTGAGGATGG+TGG	0.710714	4.4:+80133827	MS.gene80770:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TAATTTTTTTTTTTTTTAAA+AGG	+	chr4.4:80133347-80133366	MS.gene80770:intron	0.0%
!!!	ATATTTATATTTCTTTTATG+AGG	+	chr4.4:80133211-80133230	MS.gene80770:intron	10.0%
!!!	TATAATTGATTTTTATTACT+TGG	+	chr4.4:80133695-80133714	MS.gene80770:intron	10.0%
!!!	TATTTATATTTCTTTTATGA+GGG	+	chr4.4:80133212-80133231	MS.gene80770:intron	10.0%
!!	AATACTCATATAATTATGTT+TGG	-	chr4.4:80132895-80132914	None:intergenic	15.0%
!!	GATCATTAAATTATTATACT+TGG	+	chr4.4:80133141-80133160	MS.gene80770:intron	15.0%
!!!	TGTGTATAATTGATTTTTAA+AGG	+	chr4.4:80133656-80133675	MS.gene80770:intron	15.0%
!!!	TTTTAATTTATGATGATTCT+TGG	+	chr4.4:80132931-80132950	MS.gene80770:intron	15.0%
!!	AAAGCAATAACAAAAAGTTA+AGG	-	chr4.4:80132872-80132891	None:intergenic	20.0%
!!!	AAATTTGATTTGTGTATAGA+AGG	+	chr4.4:80133186-80133205	MS.gene80770:intron	20.0%
!!!	AATTTGATTTGTGTATAGAA+GGG	+	chr4.4:80133187-80133206	MS.gene80770:intron	20.0%
!!!	TAATTCATCGTTATCAATTT+TGG	+	chr4.4:80133498-80133517	MS.gene80770:intron	20.0%
!!!	TTTTCATTACTCTTTTTGTT+GGG	+	chr4.4:80132968-80132987	MS.gene80770:intron	20.0%
!	CAATTATACACATGAAACAT+TGG	-	chr4.4:80133683-80133702	None:intergenic	25.0%
!!	GTTTTCATTACTCTTTTTGT+TGG	+	chr4.4:80132967-80132986	MS.gene80770:intron	25.0%
!!	TTTTTCTTCTCTATCTTCAT+AGG	+	chr4.4:80133008-80133027	MS.gene80770:intron	25.0%
	ATAAGATATACACAACGACT+TGG	+	chr4.4:80133400-80133419	MS.gene80770:intron	30.0%
	GCAATAACAAAAAGTTAAGG+TGG	-	chr4.4:80132869-80132888	None:intergenic	30.0%
!!	AACTCTTTACCATTGACTTA+AGG	-	chr4.4:80133434-80133453	None:intergenic	30.0%
!!	AAGCACAATCATTTAGTTTG+AGG	-	chr4.4:80133577-80133596	None:intergenic	30.0%
!!	AGCACAATCATTTAGTTTGA+GGG	-	chr4.4:80133576-80133595	None:intergenic	30.0%
!	AGCACCTTCAAATTGGATTT+TGG	-	chr4.4:80133801-80133820	None:intergenic	35.0%
!!	CAATTTGAAGGTGCTGAAAT+GGG	+	chr4.4:80133806-80133825	MS.gene80770:CDS	35.0%
	CCAGCCAAAATCCAATTTGA+AGG	+	chr4.4:80133794-80133813	MS.gene80770:CDS	40.0%
	CCATTTCAGCACCTTCAAAT+TGG	-	chr4.4:80133808-80133827	None:intergenic	40.0%
!	AACCACAGAAACCGTGATTT+TGG	+	chr4.4:80133763-80133782	MS.gene80770:CDS	40.0%
!	ACCACAGAAACCGTGATTTT+GGG	+	chr4.4:80133764-80133783	MS.gene80770:CDS	40.0%
!!	CCAATTTGAAGGTGCTGAAA+TGG	+	chr4.4:80133805-80133824	MS.gene80770:CDS	40.0%
!!	CCTTCAAATTGGATTTTGGC+TGG	-	chr4.4:80133797-80133816	None:intergenic	40.0%
	GGTTTCTGTGGTTTCACCTT+CGG	-	chr4.4:80133756-80133775	None:intergenic	45.0%
	GTGCGTACTCCTTAAGTCAA+TGG	+	chr4.4:80133422-80133441	MS.gene80770:intron	45.0%
	TGAAATGGGCGTTACATCTG+AGG	+	chr4.4:80133820-80133839	MS.gene80770:CDS	45.0%
	TGTGGTGATAGCTGCAAGTA+AGG	+	chr4.4:80132838-80132857	MS.gene80770:intron	45.0%
	AGATGCAACACGAGGTCTAG+TGG	+	chr4.4:80133719-80133738	MS.gene80770:intron	50.0%
	ATGGGCGTTACATCTGAGGA+TGG	+	chr4.4:80133824-80133843	MS.gene80770:CDS	50.0%
	GCCCAAAATCACGGTTTCTG+TGG	-	chr4.4:80133768-80133787	None:intergenic	50.0%
	TACTTGGCAGATGCAACACG+AGG	+	chr4.4:80133711-80133730	MS.gene80770:intron	50.0%
	TGTGGAAGCAGTTGCAACTG+TGG	+	chr4.4:80132820-80132839	MS.gene80770:CDS	50.0%
!	GAAACCGTGATTTTGGGCGT+CGG	+	chr4.4:80133770-80133789	MS.gene80770:CDS	50.0%
!!	AGTGGATTGAGCTATGCCGA+AGG	+	chr4.4:80133737-80133756	MS.gene80770:CDS	50.0%
	GGCGTTACATCTGAGGATGG+TGG	+	chr4.4:80133827-80133846	MS.gene80770:CDS	55.0%
	TGGACCGACGCCCAAAATCA+CGG	-	chr4.4:80133777-80133796	None:intergenic	55.0%
!	GAGGATGGTGGCTGCAAGTG+TGG	+	chr4.4:80133839-80133858	MS.gene80770:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.4	gene	80132805	80133898	80132805	ID=MS.gene80770
chr4.4	mRNA	80132805	80133898	80132805	ID=MS.gene80770.t1;Parent=MS.gene80770
chr4.4	exon	80132805	80132854	80132805	ID=MS.gene80770.t1.exon1;Parent=MS.gene80770.t1
chr4.4	CDS	80132805	80132854	80132805	ID=cds.MS.gene80770.t1;Parent=MS.gene80770.t1
chr4.4	exon	80133721	80133898	80133721	ID=MS.gene80770.t1.exon2;Parent=MS.gene80770.t1
chr4.4	CDS	80133721	80133898	80133721	ID=cds.MS.gene80770.t1;Parent=MS.gene80770.t1

Gene Sequence

>MS.gene80770

ATGTCTAGCTGTGGTTGTGGAAGCAGTTGCAACTGTGGTGATAGCTGCAAGTAAGGATCGACCACCTTAACTTTTTGTTATTGCTTTCCAAACATAATTATATGAGTATTTTAATTTGTGTTTATTTTTTAATTTATGATGATTCTTGGATATTTTGTTTGTGTTTTCATTACTCTTTTTGTTGGGTTCTATGAATTTGATGCTTTTTCTTCTCTATCTTCATAGGATTTCTAGAGTTGAGTTTTAGTCTTTTTCATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTTAATACTTTGATCATTAAATTATTATACTTGGTCACTAGCAGATGATTTTGATGAAATTTGATTTGTGTATAGAAGGGAATATTTATATTTCTTTTATGAGGGTTTAAAAAAAAAACTTGAAAAACTAGATCATGAATCATAATATATTCGTGCTTGTGTTTCAACTTTATTTATGTAAATTTCTAAAACTTGTCAATAGAAACAAGTATGAGCTTAATTTTTTTTTTTTTTAAAAGGCTTAAATAGTCTATTAAAAGTGTCTTTCTAATAAGATATACACAACGACTTGGTGCGTACTCCTTAAGTCAATGGTAAAGAGTTAAATATTAGACTTTAGTGTGTGAAGAAAATTAAGTGTACAATAATAATTCATCGTTATCAATTTTGGTTAATTCTTCTTAAAAAAATAAGTGTACAATAATATAGTTATTAAATCTGATCCCTCAAACTAAATGATTGTGCTTAAAGTTTATTCTTTCTTTGATATTTATCATGTAAACAATTATTTGCAGTGTATAATTGATGTGTATAATTGATTTTTAAAGGTCCAATGTTTCATGTGTATAATTGATTTTTATTACTTGGCAGATGCAACACGAGGTCTAGTGGATTGAGCTATGCCGAAGGTGAAACCACAGAAACCGTGATTTTGGGCGTCGGTCCAGCCAAAATCCAATTTGAAGGTGCTGAAATGGGCGTTACATCTGAGGATGGTGGCTGCAAGTGTGGAGATAACTGCACATGTGACCCTTGCAACTGCAAATGA

Protein sequence

>MS.gene80770.t1

MSSCGCGSSCNCGDSCKCNTRSSGLSYAEGETTETVILGVGPAKIQFEGAEMGVTSEDGGCKCGDNCTCDPCNCK