AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene70586


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene70586.t1 MTR_3g078590 100.000 90 0 0 1 90 1 90 2.56e-63 186
MS.gene70586.t1 MTR_3g085890 69.318 88 27 0 1 88 1 88 1.66e-43 136
MS.gene70586.t1 MTR_8g080060 69.318 88 27 0 1 88 1 88 4.04e-43 135
MS.gene70586.t1 MTR_7g016060 51.220 82 40 0 5 86 3 84 3.47e-29 100
MS.gene70586.t1 MTR_3g085890 63.934 61 22 0 27 87 1 61 1.80e-25 90.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene70586.t1 AT3G48140 74.118 85 22 0 4 88 3 87 1.26e-44 139
MS.gene70586.t1 AT3G29970 51.220 82 40 0 5 86 3 84 8.05e-30 101
MS.gene70586.t1 AT3G29970 47.059 68 36 0 18 85 1 68 2.24e-20 77.4
MS.gene70586.t1 AT3G29970 47.059 68 36 0 18 85 1 68 2.24e-20 77.4

Find 28 sgRNAs with CRISPR-Local

Find 75 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAACATCAGTACCTGACTTC+AGG 0.347160 3.1:+64388285 None:intergenic
TACCCTCTGTTTGCTGCGGT+TGG 0.396092 3.1:-64388360 MS.gene70586:CDS
CTGCGGTTGGAGCTGCTGTT+GGG 0.406881 3.1:-64388347 MS.gene70586:CDS
GCTGCGGTTGGAGCTGCTGT+TGG 0.426937 3.1:-64388348 MS.gene70586:CDS
TTGAGGCCGGGCATAATCTC+TGG 0.440854 3.1:+64387306 None:intergenic
AACATCAGTACCTGACTTCA+GGG 0.462667 3.1:+64388286 None:intergenic
AACAAGCAGTCTCGAGCTGC+AGG 0.464020 3.1:-64387402 MS.gene70586:CDS
AGTGAAGAAGCTGTTGAGGC+CGG 0.469984 3.1:+64387293 None:intergenic
GTCTCGAGCTGCAGGAGTGT+TGG 0.483795 3.1:-64387394 MS.gene70586:CDS
GGGTCTGATCCAGCGATTGG+TGG 0.513757 3.1:+64388474 None:intergenic
AAATAACTTAGTTGCGACTT+GGG 0.516597 3.1:+64387268 None:intergenic
TTCTGTGTACTTCTCTCCCT+CGG 0.519035 3.1:+64387362 None:intergenic
CTCGGGTCTGATCCAGCGAT+TGG 0.519677 3.1:+64388471 None:intergenic
GTGAAGAAGCTGTTGAGGCC+GGG 0.521542 3.1:+64387294 None:intergenic
TATCCATGGCCACCAATCGC+TGG 0.522115 3.1:-64388483 None:intergenic
TCTGCATCAACCCTGAAGTC+AGG 0.524860 3.1:-64388296 MS.gene70586:intron
AAAATAACTTAGTTGCGACT+TGG 0.526355 3.1:+64387267 None:intergenic
GATGTTACGAACAAGTGAAA+AGG 0.535731 3.1:+64388317 None:intergenic
AGATCACCAGAGATTATGCC+CGG 0.537901 3.1:-64387312 MS.gene70586:CDS
GATCCAGCGATTGGTGGCCA+TGG 0.584846 3.1:+64388480 None:intergenic
CTCCAACCGCAGCAAACAGA+GGG 0.612181 3.1:+64388358 None:intergenic
GTGTTGGAGAACTTTGCCGA+GGG 0.614956 3.1:-64387378 MS.gene70586:CDS
GGTATACCCTCTGTTTGCTG+CGG 0.632424 3.1:-64388364 MS.gene70586:CDS
AGTGTTGGAGAACTTTGCCG+AGG 0.642190 3.1:-64387379 MS.gene70586:CDS
GGTCAGTGAAGAAGCTGTTG+AGG 0.648101 3.1:+64387289 None:intergenic
GCTCCAACCGCAGCAAACAG+AGG 0.651727 3.1:+64388357 None:intergenic
CAATCGCTGGATCAGACCCG+AGG 0.698764 3.1:-64388470 MS.gene70586:intron
AGTACACAGAACATTTGCTG+AGG 0.706764 3.1:-64387350 MS.gene70586:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGATATTAACAAAATTTAAT+GGG - chr3.1:64387706-64387725 MS.gene70586:intron 10.0%
!! TAGATATTAACAAAATTTAA+TGG - chr3.1:64387705-64387724 MS.gene70586:intron 10.0%
!! ATTAATCTCTAATATCAAAT+AGG + chr3.1:64387957-64387976 None:intergenic 15.0%
!! TTAATCTCTAATATCAAATA+GGG + chr3.1:64387956-64387975 None:intergenic 15.0%
!!! AGAGATTAATTTTTATTCAT+TGG - chr3.1:64387967-64387986 MS.gene70586:intron 15.0%
!!! GAATATTCCTTTTTTATTTT+TGG - chr3.1:64387626-64387645 MS.gene70586:intron 15.0%
!! AATTAAATAACTCATGTATC+TGG - chr3.1:64387778-64387797 MS.gene70586:intron 20.0%
!! ACAAATTGTGAAAATTTAAG+CGG + chr3.1:64387682-64387701 None:intergenic 20.0%
!! AGGAAAACCAAAAATAAAAA+AGG + chr3.1:64387636-64387655 None:intergenic 20.0%
!! CTAATCGGATAATTAATTAA+TGG + chr3.1:64387595-64387614 None:intergenic 20.0%
!! GATACATGAGTTATTTAATT+TGG + chr3.1:64387778-64387797 None:intergenic 20.0%
!! TATACCTATAACAAAAAAGA+TGG + chr3.1:64387375-64387394 None:intergenic 20.0%
!!! GATTAATTTTTATTCATTGG+AGG - chr3.1:64387970-64387989 MS.gene70586:intron 20.0%
!!! TCCTAAAAATCACTTTTTTA+AGG + chr3.1:64387826-64387845 None:intergenic 20.0%
! ATTCCATCATGTTTACTAAT+CGG + chr3.1:64387610-64387629 None:intergenic 25.0%
! TGAGATATAACTTAAAAGAG+TGG - chr3.1:64388046-64388065 MS.gene70586:intron 25.0%
!! AATGAAAACTTTTATCTCAC+AGG - chr3.1:64388236-64388255 MS.gene70586:intron 25.0%
!! ATGAAAACTTTTATCTCACA+GGG - chr3.1:64388237-64388256 MS.gene70586:intron 25.0%
!! ATGTATGTTTGTTCTTTTGA+TGG - chr3.1:64388318-64388337 MS.gene70586:CDS 25.0%
!! GCCTTAAAAAAGTGATTTTT+AGG - chr3.1:64387822-64387841 MS.gene70586:intron 25.0%
!! TGTATGTTTGTTCTTTTGAT+GGG - chr3.1:64388319-64388338 MS.gene70586:CDS 25.0%
!!! AATGGTGATTTTAAAGTTGT+TGG - chr3.1:64387927-64387946 MS.gene70586:intron 25.0%
!!! TGATTGATTTTTCTCTTCTT+TGG - chr3.1:64387521-64387540 MS.gene70586:intron 25.0%
!!! TTCACCATCTTTTTTGTTAT+AGG - chr3.1:64387368-64387387 MS.gene70586:CDS 25.0%
!!! TTGTTGTTTAATTTTTAGCC+AGG - chr3.1:64388269-64388288 MS.gene70586:intron 25.0%
!!! TTTAATTTTTAGCCAGGATT+TGG - chr3.1:64388275-64388294 MS.gene70586:intron 25.0%
ACGAAATGCAAAGTAGTAAA+TGG + chr3.1:64387334-64387353 None:intergenic 30.0%
CAATCATCGGATTATTATCA+TGG + chr3.1:64387858-64387877 None:intergenic 30.0%
CATCATATCATTTCTCAGAT+AGG + chr3.1:64387656-64387675 None:intergenic 30.0%
TATCCGATTAGTAAACATGA+TGG - chr3.1:64387604-64387623 MS.gene70586:intron 30.0%
!!! TTGTTCTTTTGATGGGTTTA+GGG - chr3.1:64388326-64388345 MS.gene70586:CDS 30.0%
!!! TTTGTTCTTTTGATGGGTTT+AGG - chr3.1:64388325-64388344 MS.gene70586:CDS 30.0%
AAATCCATGATATGTTTGCC+TGG - chr3.1:64388134-64388153 MS.gene70586:intron 35.0%
AACTTAAAAGAGTGGCATCA+TGG - chr3.1:64388054-64388073 MS.gene70586:intron 35.0%
ACTCATGTATCTGGACTAAA+TGG - chr3.1:64387787-64387806 MS.gene70586:intron 35.0%
GACAAATCAGCTTCAATCAT+CGG + chr3.1:64387871-64387890 None:intergenic 35.0%
GATGTTACGAACAAGTGAAA+AGG + chr3.1:64387439-64387458 None:intergenic 35.0%
TGCATTTCGTAAGAGAGTTA+GGG - chr3.1:64387344-64387363 MS.gene70586:CDS 35.0%
TGGCAAAATACAGATCGAAA+AGG + chr3.1:64387314-64387333 None:intergenic 35.0%
TTGCATTTCGTAAGAGAGTT+AGG - chr3.1:64387343-64387362 MS.gene70586:CDS 35.0%
TTTGCACAAATAACGTGGAA+TGG - chr3.1:64387909-64387928 MS.gene70586:intron 35.0%
! CGAATTTTGCACAAATAACG+TGG - chr3.1:64387904-64387923 MS.gene70586:intron 35.0%
! GAGTTTGACTTATCTCAGAT+CGG - chr3.1:64387543-64387562 MS.gene70586:intron 35.0%
! TGGTGTTTGAAGGAGTATAA+TGG - chr3.1:64388295-64388314 MS.gene70586:intron 35.0%
AAACATCAGTACCTGACTTC+AGG + chr3.1:64387471-64387490 None:intergenic 40.0%
AACATCAGTACCTGACTTCA+GGG + chr3.1:64387470-64387489 None:intergenic 40.0%
AAGTCGCTTATAGAGAGAGT+AGG + chr3.1:64387747-64387766 None:intergenic 40.0%
CAACTTCATTTCACTCAACC+AGG + chr3.1:64388155-64388174 None:intergenic 40.0%
CTGGTTGAGTGAAATGAAGT+TGG - chr3.1:64388153-64388172 MS.gene70586:intron 40.0%
TCAACCAGGCAAACATATCA+TGG + chr3.1:64388141-64388160 None:intergenic 40.0%
! AGTACACAGAACATTTGCTG+AGG - chr3.1:64388403-64388422 MS.gene70586:intron 40.0%
AGATCACCAGAGATTATGCC+CGG - chr3.1:64388441-64388460 MS.gene70586:intron 45.0%
AGATCGAAAAGGACTCACCT+CGG + chr3.1:64387303-64387322 None:intergenic 45.0%
CTCCTTCAAACACCAAATCC+TGG + chr3.1:64388290-64388309 None:intergenic 45.0%
TTCTGTGTACTTCTCTCCCT+CGG + chr3.1:64388394-64388413 None:intergenic 45.0%
!! AGCCAGGATTTGGTGTTTGA+AGG - chr3.1:64388285-64388304 MS.gene70586:intron 45.0%
AGTGAAGAAGCTGTTGAGGC+CGG + chr3.1:64388463-64388482 None:intergenic 50.0%
GATCGAAAAGGACTCACCTC+GGG + chr3.1:64387302-64387321 None:intergenic 50.0%
GGTCAGTGAAGAAGCTGTTG+AGG + chr3.1:64388467-64388486 None:intergenic 50.0%
GTGTTGGAGAACTTTGCCGA+GGG - chr3.1:64388375-64388394 MS.gene70586:intron 50.0%
TCTGCATCAACCCTGAAGTC+AGG - chr3.1:64387457-64387476 MS.gene70586:intron 50.0%
! AGTGTTGGAGAACTTTGCCG+AGG - chr3.1:64388374-64388393 MS.gene70586:intron 50.0%
! GGTATACCCTCTGTTTGCTG+CGG - chr3.1:64387389-64387408 MS.gene70586:CDS 50.0%
AACAAGCAGTCTCGAGCTGC+AGG - chr3.1:64388351-64388370 MS.gene70586:CDS 55.0%
CTCCAACCGCAGCAAACAGA+GGG + chr3.1:64387398-64387417 None:intergenic 55.0%
GTGAAGAAGCTGTTGAGGCC+GGG + chr3.1:64388462-64388481 None:intergenic 55.0%
TTGAGGCCGGGCATAATCTC+TGG + chr3.1:64388450-64388469 None:intergenic 55.0%
! TACCCTCTGTTTGCTGCGGT+TGG - chr3.1:64387393-64387412 MS.gene70586:CDS 55.0%
CAATCGCTGGATCAGACCCG+AGG - chr3.1:64387283-64387302 MS.gene70586:CDS 60.0%
CTCGGGTCTGATCCAGCGAT+TGG + chr3.1:64387285-64387304 None:intergenic 60.0%
CTGCGGTTGGAGCTGCTGTT+GGG - chr3.1:64387406-64387425 MS.gene70586:CDS 60.0%
GCTCCAACCGCAGCAAACAG+AGG + chr3.1:64387399-64387418 None:intergenic 60.0%
GGGTCTGATCCAGCGATTGG+TGG + chr3.1:64387282-64387301 None:intergenic 60.0%
! GTCTCGAGCTGCAGGAGTGT+TGG - chr3.1:64388359-64388378 MS.gene70586:CDS 60.0%
GCTGCGGTTGGAGCTGCTGT+TGG - chr3.1:64387405-64387424 MS.gene70586:CDS 65.0%


Chromosome Type Strat End Strand Name
chr3.1 gene 64387275 64388500 64387275 ID=MS.gene70586
chr3.1 mRNA 64387275 64388500 64387275 ID=MS.gene70586.t1;Parent=MS.gene70586
chr3.1 exon 64388471 64388500 64388471 ID=MS.gene70586.t1.exon1;Parent=MS.gene70586.t1
chr3.1 CDS 64388471 64388500 64388471 ID=cds.MS.gene70586.t1;Parent=MS.gene70586.t1
chr3.1 exon 64388297 64388385 64388297 ID=MS.gene70586.t1.exon2;Parent=MS.gene70586.t1
chr3.1 CDS 64388297 64388385 64388297 ID=cds.MS.gene70586.t1;Parent=MS.gene70586.t1
chr3.1 exon 64387275 64387428 64387275 ID=MS.gene70586.t1.exon3;Parent=MS.gene70586.t1
chr3.1 CDS 64387275 64387428 64387275 ID=cds.MS.gene70586.t1;Parent=MS.gene70586.t1
Gene Sequence

>MS.gene70586

ATGGCCACCAATCGCTGGATCAGACCCGAGGTGAGTCCTTTTCGATCTGTATTTTGCCATTTACTACTTTGCATTTCGTAAGAGAGTTAGGGTTTCACCATCTTTTTTGTTATAGGTATACCCTCTGTTTGCTGCGGTTGGAGCTGCTGTTGGGATCTGTGCCTTTTCACTTGTTCGTAACATCTGCATCAACCCTGAAGTCAGGTACTGATGTTTTTTATTTTTTTATTTTATCTTTGAGATTATTGATTGATTTTTCTCTTCTTTGGAGTTTGACTTATCTCAGATCGGTGATTAATACTGCTAATAATTGCTTTCCATTAATTAATTATCCGATTAGTAAACATGATGGAATATTCCTTTTTTATTTTTGGTTTTCCTATCTGAGAAATGATATGATGTATCCGCTTAAATTTTCACAATTTGTTTCTAGATATTAACAAAATTTAATGGGATTCTATTTTAGATACCTACTCTCTCTATAAGCGACTTTTTTTTTTCCAAATTAAATAACTCATGTATCTGGACTAAATGGATTTGATTAACTGCCTTAAAAAAGTGATTTTTAGGAGATCTTTAACCATGATAATAATCCGATGATTGAAGCTGATTTGTCAAATTTATTAAAGCGAATTTTGCACAAATAACGTGGAATGGTGATTTTAAAGTTGTTGGCTACCCTATTTGATATTAGAGATTAATTTTTATTCATTGGAGGTGTAAATTTTGTTTTACACGTGCATCTAATCAAATCACGTCAAACTGATATTTTGAGATATAACTTAAAAGAGTGGCATCATGGTGTGATTTTATTGAATGCATCTGTAAAAAAGTTTTACACTGTCAGTGTATACAAGTTAAATCCATGATATGTTTGCCTGGTTGAGTGAAATGAAGTTGGAGATATTCAATTGCTTGAGATTATTTAAATTATGTCTTGACAGAACTGCTATCTATTGAAAATGAAAACTTTTATCTCACAGGGATGTAGCTATTGTTGTTTAATTTTTAGCCAGGATTTGGTGTTTGAAGGAGTATAATGGATGTATGTTTGTTCTTTTGATGGGTTTAGGGTGAACAAGCAGTCTCGAGCTGCAGGAGTGTTGGAGAACTTTGCCGAGGGAGAGAAGTACACAGAACATTTGCTGAGGAAGTTTTCACGCAACAGATCACCAGAGATTATGCCCGGCCTCAACAGCTTCTTCACTGACCCAAGTCGCAACTAA

Protein sequence

>MS.gene70586.t1

MATNRWIRPEVYPLFAAVGAAVGICAFSLVRNICINPEVRVNKQSRAAGVLENFAEGEKYTEHLLRKFSRNRSPEIMPGLNSFFTDPSRN