AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene25913


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene25913.t1 MTR_3g078590 100.000 90 0 0 1 90 1 90 2.56e-63 186
MS.gene25913.t1 MTR_3g085890 69.318 88 27 0 1 88 1 88 1.66e-43 136
MS.gene25913.t1 MTR_8g080060 69.318 88 27 0 1 88 1 88 4.04e-43 135
MS.gene25913.t1 MTR_7g016060 51.220 82 40 0 5 86 3 84 3.47e-29 100
MS.gene25913.t1 MTR_3g085890 63.934 61 22 0 27 87 1 61 1.80e-25 90.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene25913.t1 AT3G48140 74.118 85 22 0 4 88 3 87 1.26e-44 139
MS.gene25913.t1 AT3G29970 51.220 82 40 0 5 86 3 84 8.05e-30 101
MS.gene25913.t1 AT3G29970 47.059 68 36 0 18 85 1 68 2.24e-20 77.4
MS.gene25913.t1 AT3G29970 47.059 68 36 0 18 85 1 68 2.24e-20 77.4

Find 28 sgRNAs with CRISPR-Local

Find 73 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAACATCAGTACCTGACTTC+AGG 0.347160 3.2:+69345181 None:intergenic
TACCCTCTGTTTGCTGCGGT+TGG 0.396092 3.2:-69345256 MS.gene25913:CDS
CTGCGGTTGGAGCTGCTGTT+GGG 0.406881 3.2:-69345243 MS.gene25913:CDS
GCTGCGGTTGGAGCTGCTGT+TGG 0.426937 3.2:-69345244 MS.gene25913:CDS
TTGAGGCCGGGCATAATCTC+TGG 0.440854 3.2:+69344209 None:intergenic
AACATCAGTACCTGACTTCA+GGG 0.462667 3.2:+69345182 None:intergenic
AACAAGCAGTCTCGAGCTGC+AGG 0.464020 3.2:-69344305 MS.gene25913:CDS
AGTGAAGAAGCTGTTGAGGC+CGG 0.469984 3.2:+69344196 None:intergenic
GTCTCGAGCTGCAGGAGTGT+TGG 0.483795 3.2:-69344297 MS.gene25913:CDS
GGGTCTGATCCAGCGATTGG+TGG 0.513757 3.2:+69345370 None:intergenic
AAATAACTTAGTTGCGACTT+GGG 0.516597 3.2:+69344171 None:intergenic
TTCTGTGTACTTCTCTCCCT+CGG 0.519035 3.2:+69344265 None:intergenic
CTCGGGTCTGATCCAGCGAT+TGG 0.519677 3.2:+69345367 None:intergenic
GTGAAGAAGCTGTTGAGGCC+GGG 0.521542 3.2:+69344197 None:intergenic
TATCCATGGCCACCAATCGC+TGG 0.522115 3.2:-69345379 None:intergenic
TCTGCATCAACCCTGAAGTC+AGG 0.524860 3.2:-69345192 MS.gene25913:intron
AAAATAACTTAGTTGCGACT+TGG 0.526355 3.2:+69344170 None:intergenic
GATGTTACGAACAAGTGAAA+AGG 0.535731 3.2:+69345213 None:intergenic
AGATCACCAGAGATTATGCC+CGG 0.537901 3.2:-69344215 MS.gene25913:CDS
GATCCAGCGATTGGTGGCCA+TGG 0.584846 3.2:+69345376 None:intergenic
CTCCAACCGCAGCAAACAGA+GGG 0.612181 3.2:+69345254 None:intergenic
GTGTTGGAGAACTTTGCCGA+GGG 0.614956 3.2:-69344281 MS.gene25913:CDS
GGTATACCCTCTGTTTGCTG+CGG 0.632424 3.2:-69345260 MS.gene25913:CDS
AGTGTTGGAGAACTTTGCCG+AGG 0.642190 3.2:-69344282 MS.gene25913:CDS
GGTCAGTGAAGAAGCTGTTG+AGG 0.648101 3.2:+69344192 None:intergenic
GCTCCAACCGCAGCAAACAG+AGG 0.651727 3.2:+69345253 None:intergenic
CAATCGCTGGATCAGACCCG+AGG 0.698764 3.2:-69345366 MS.gene25913:intron
AGTACACAGAACATTTGCTG+AGG 0.706764 3.2:-69344253 MS.gene25913:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGATATTAACAAAATTTAAT+GGG - chr3.2:69344609-69344628 MS.gene25913:intron 10.0%
!! TAGATATTAACAAAATTTAA+TGG - chr3.2:69344608-69344627 MS.gene25913:intron 10.0%
!! ATTAATCTCTAATATCAAAT+AGG + chr3.2:69344853-69344872 None:intergenic 15.0%
!! TTAATCTCTAATATCAAATA+GGG + chr3.2:69344852-69344871 None:intergenic 15.0%
!!! AGAGATTAATTTTTATTCAT+TGG - chr3.2:69344863-69344882 MS.gene25913:intron 15.0%
!!! GAATATTCCTTTTTTATTTT+TGG - chr3.2:69344529-69344548 MS.gene25913:intron 15.0%
!! AATTAAATAACTCATGTATC+TGG - chr3.2:69344683-69344702 MS.gene25913:intron 20.0%
!! ACAAATTGTGAAAATTTAAG+CGG + chr3.2:69344585-69344604 None:intergenic 20.0%
!! AGGAAAACCAAAAATAAAAA+AGG + chr3.2:69344539-69344558 None:intergenic 20.0%
!! CTAATCGGATAATTAATTAA+TGG + chr3.2:69344498-69344517 None:intergenic 20.0%
!! GATACATGAGTTATTTAATT+TGG + chr3.2:69344683-69344702 None:intergenic 20.0%
!! TATACCTATAACAAAAAAGA+TGG + chr3.2:69344278-69344297 None:intergenic 20.0%
!!! GATTAATTTTTATTCATTGG+AGG - chr3.2:69344866-69344885 MS.gene25913:intron 20.0%
!!! TGATTAACTAAGTGATTTTT+AGG - chr3.2:69344718-69344737 MS.gene25913:intron 20.0%
! ATTCCATCATGTTTACTAAT+CGG + chr3.2:69344513-69344532 None:intergenic 25.0%
! TGAGATATAACTTAAAAGAG+TGG - chr3.2:69344942-69344961 MS.gene25913:intron 25.0%
! TTTGCTCAAATAACGTTTAA+TGG - chr3.2:69344805-69344824 MS.gene25913:intron 25.0%
!! AATGAAAACTTTTATCTCAC+AGG - chr3.2:69345132-69345151 MS.gene25913:intron 25.0%
!! ATGAAAACTTTTATCTCACA+GGG - chr3.2:69345133-69345152 MS.gene25913:intron 25.0%
!! ATGTATGTTTGTTCTTTTGA+TGG - chr3.2:69345214-69345233 MS.gene25913:CDS 25.0%
!! TGTATGTTTGTTCTTTTGAT+GGG - chr3.2:69345215-69345234 MS.gene25913:CDS 25.0%
!!! AATGGTGATTTTAAAGTTGT+TGG - chr3.2:69344823-69344842 MS.gene25913:intron 25.0%
!!! TGATTGATTTTTCTCTTCTT+TGG - chr3.2:69344424-69344443 MS.gene25913:intron 25.0%
!!! TTCACCATCTTTTTTGTTAT+AGG - chr3.2:69344271-69344290 MS.gene25913:CDS 25.0%
!!! TTGTTGTTTAATTTTTAGCC+AGG - chr3.2:69345165-69345184 MS.gene25913:intron 25.0%
!!! TTTAATTTTTAGCCAGGATT+TGG - chr3.2:69345171-69345190 MS.gene25913:intron 25.0%
ACGAAATGCAAAGTAGTAAA+TGG + chr3.2:69344237-69344256 None:intergenic 30.0%
CATCATATCATTTCTCAGAT+AGG + chr3.2:69344559-69344578 None:intergenic 30.0%
TATCCGATTAGTAAACATGA+TGG - chr3.2:69344507-69344526 MS.gene25913:intron 30.0%
!!! TTGTTCTTTTGATGGGTTTA+GGG - chr3.2:69345222-69345241 MS.gene25913:CDS 30.0%
!!! TTTGTTCTTTTGATGGGTTT+AGG - chr3.2:69345221-69345240 MS.gene25913:CDS 30.0%
AAATCCATGATATGTTTGCC+TGG - chr3.2:69345030-69345049 MS.gene25913:intron 35.0%
AACTTAAAAGAGTGGCATCA+TGG - chr3.2:69344950-69344969 MS.gene25913:intron 35.0%
ACTCATGTATCTGGACTAAA+TGG - chr3.2:69344692-69344711 MS.gene25913:intron 35.0%
GACAAATCAGCTTCAATCAT+CGG + chr3.2:69344767-69344786 None:intergenic 35.0%
GATGTTACGAACAAGTGAAA+AGG + chr3.2:69344342-69344361 None:intergenic 35.0%
TGCATTTCGTAAGAGAGTTA+GGG - chr3.2:69344247-69344266 MS.gene25913:CDS 35.0%
TGGCAAAATACAGATCGAAA+AGG + chr3.2:69344217-69344236 None:intergenic 35.0%
TTGCATTTCGTAAGAGAGTT+AGG - chr3.2:69344246-69344265 MS.gene25913:CDS 35.0%
! GAGTTTGACTTATCTCAGAT+CGG - chr3.2:69344446-69344465 MS.gene25913:intron 35.0%
AAACATCAGTACCTGACTTC+AGG + chr3.2:69344374-69344393 None:intergenic 40.0%
AACATCAGTACCTGACTTCA+GGG + chr3.2:69344373-69344392 None:intergenic 40.0%
AAGTCGCTTATAGAGAGAGT+AGG + chr3.2:69344651-69344670 None:intergenic 40.0%
CTGGTTGAGTGAAATGAAGT+CGG - chr3.2:69345049-69345068 MS.gene25913:intron 40.0%
TCAACCAGGCAAACATATCA+TGG + chr3.2:69345037-69345056 None:intergenic 40.0%
TGGTGTTTGAAGGAGGATAA+TGG - chr3.2:69345191-69345210 MS.gene25913:intron 40.0%
! AGTACACAGAACATTTGCTG+AGG - chr3.2:69345299-69345318 MS.gene25913:intron 40.0%
AGATCACCAGAGATTATGCC+CGG - chr3.2:69345337-69345356 MS.gene25913:intron 45.0%
AGATCGAAAAGGACTCACCT+CGG + chr3.2:69344206-69344225 None:intergenic 45.0%
CGACTTCATTTCACTCAACC+AGG + chr3.2:69345051-69345070 None:intergenic 45.0%
CTCCTTCAAACACCAAATCC+TGG + chr3.2:69345186-69345205 None:intergenic 45.0%
TTCTGTGTACTTCTCTCCCT+CGG + chr3.2:69345290-69345309 None:intergenic 45.0%
!! AGCCAGGATTTGGTGTTTGA+AGG - chr3.2:69345181-69345200 MS.gene25913:intron 45.0%
!! CAGGATTTGGTGTTTGAAGG+AGG - chr3.2:69345184-69345203 MS.gene25913:intron 45.0%
AGTGAAGAAGCTGTTGAGGC+CGG + chr3.2:69345359-69345378 None:intergenic 50.0%
GATCGAAAAGGACTCACCTC+GGG + chr3.2:69344205-69344224 None:intergenic 50.0%
GGTCAGTGAAGAAGCTGTTG+AGG + chr3.2:69345363-69345382 None:intergenic 50.0%
GTGTTGGAGAACTTTGCCGA+GGG - chr3.2:69345271-69345290 MS.gene25913:intron 50.0%
TCTGCATCAACCCTGAAGTC+AGG - chr3.2:69344360-69344379 MS.gene25913:intron 50.0%
! AGTGTTGGAGAACTTTGCCG+AGG - chr3.2:69345270-69345289 MS.gene25913:intron 50.0%
! GGTATACCCTCTGTTTGCTG+CGG - chr3.2:69344292-69344311 MS.gene25913:CDS 50.0%
AACAAGCAGTCTCGAGCTGC+AGG - chr3.2:69345247-69345266 MS.gene25913:CDS 55.0%
CTCCAACCGCAGCAAACAGA+GGG + chr3.2:69344301-69344320 None:intergenic 55.0%
GTGAAGAAGCTGTTGAGGCC+GGG + chr3.2:69345358-69345377 None:intergenic 55.0%
TTGAGGCCGGGCATAATCTC+TGG + chr3.2:69345346-69345365 None:intergenic 55.0%
! TACCCTCTGTTTGCTGCGGT+TGG - chr3.2:69344296-69344315 MS.gene25913:CDS 55.0%
CAATCGCTGGATCAGACCCG+AGG - chr3.2:69344186-69344205 MS.gene25913:CDS 60.0%
CTCGGGTCTGATCCAGCGAT+TGG + chr3.2:69344188-69344207 None:intergenic 60.0%
CTGCGGTTGGAGCTGCTGTT+GGG - chr3.2:69344309-69344328 MS.gene25913:CDS 60.0%
GCTCCAACCGCAGCAAACAG+AGG + chr3.2:69344302-69344321 None:intergenic 60.0%
GGGTCTGATCCAGCGATTGG+TGG + chr3.2:69344185-69344204 None:intergenic 60.0%
! GTCTCGAGCTGCAGGAGTGT+TGG - chr3.2:69345255-69345274 MS.gene25913:CDS 60.0%
GCTGCGGTTGGAGCTGCTGT+TGG - chr3.2:69344308-69344327 MS.gene25913:CDS 65.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 69344178 69345396 69344178 ID=MS.gene25913
chr3.2 mRNA 69344178 69345396 69344178 ID=MS.gene25913.t1;Parent=MS.gene25913
chr3.2 exon 69345367 69345396 69345367 ID=MS.gene25913.t1.exon1;Parent=MS.gene25913.t1
chr3.2 CDS 69345367 69345396 69345367 ID=cds.MS.gene25913.t1;Parent=MS.gene25913.t1
chr3.2 exon 69345193 69345281 69345193 ID=MS.gene25913.t1.exon2;Parent=MS.gene25913.t1
chr3.2 CDS 69345193 69345281 69345193 ID=cds.MS.gene25913.t1;Parent=MS.gene25913.t1
chr3.2 exon 69344178 69344331 69344178 ID=MS.gene25913.t1.exon3;Parent=MS.gene25913.t1
chr3.2 CDS 69344178 69344331 69344178 ID=cds.MS.gene25913.t1;Parent=MS.gene25913.t1
Gene Sequence

>MS.gene25913

ATGGCCACCAATCGCTGGATCAGACCCGAGGTGAGTCCTTTTCGATCTGTATTTTGCCATTTACTACTTTGCATTTCGTAAGAGAGTTAGGGTTTCACCATCTTTTTTGTTATAGGTATACCCTCTGTTTGCTGCGGTTGGAGCTGCTGTTGGGATCTGTGCCTTTTCACTTGTTCGTAACATCTGCATCAACCCTGAAGTCAGGTACTGATGTTTTTTATTTTTTTATTTTATCTTTGAGATTATTGATTGATTTTTCTCTTCTTTGGAGTTTGACTTATCTCAGATCGGTGATTAATACTGCTAATAATTGCTTTCCATTAATTAATTATCCGATTAGTAAACATGATGGAATATTCCTTTTTTATTTTTGGTTTTCCTATCTGAGAAATGATATGATGTATCCGCTTAAATTTTCACAATTTGTTTGTAGATATTAACAAAATTTAATGGGATTCTATTTTTTGATACCTACTCTCTCTATAAGCGACTTTTTTTTTCTCCAAATTAAATAACTCATGTATCTGGACTAAATGGATTTGATTAACTAAGTGATTTTTAGGAGATCTTTAACTATGATAATAATCCGATGATTGAAGCTGATTTGTCAAATTTATTAAAGCGAATTTTGCTCAAATAACGTTTAATGGTGATTTTAAAGTTGTTGGCTACCCTATTTGATATTAGAGATTAATTTTTATTCATTGGAGGTGTAAATTTTGTTTTACACGTGCATCTAATCAAATCACGTCAAACTGATATTTTGAGATATAACTTAAAAGAGTGGCATCATGGTGTGATTTTATTGAATGCATCTGTAAAAATGTTTTACACTGTCAGTGTATACAAGTTAAATCCATGATATGTTTGCCTGGTTGAGTGAAATGAAGTCGGAGATATTCAATTGCTTGAGATTATTTAAATTATGTCTTGACAGAACTGCTATCTATTGAAAATGAAAACTTTTATCTCACAGGGATGTAGCTATTGTTGTTTAATTTTTAGCCAGGATTTGGTGTTTGAAGGAGGATAATGGATGTATGTTTGTTCTTTTGATGGGTTTAGGGTGAACAAGCAGTCTCGAGCTGCAGGAGTGTTGGAGAACTTTGCCGAGGGAGAGAAGTACACAGAACATTTGCTGAGGAAGTTTTCACGCAACAGATCACCAGAGATTATGCCCGGCCTCAACAGCTTCTTCACTGACCCAAGTCGCAACTAA

Protein sequence

>MS.gene25913.t1

MATNRWIRPEVYPLFAAVGAAVGICAFSLVRNICINPEVRVNKQSRAAGVLENFAEGEKYTEHLLRKFSRNRSPEIMPGLNSFFTDPSRN