AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene80557


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 25 sgRNAs with CRISPR-Local

Find 33 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGGAGACAGCAATTAATAT+TGG 0.277027 8.1:-15480768 MS.gene80557:CDS
GAGAAGGATTTGATTCTTCT+TGG 0.277424 8.1:-15481013 MS.gene80557:CDS
GGTATTGACCGGGGATAAAA+TGG 0.293752 8.1:-15480787 MS.gene80557:CDS
GCTAGTTTGTCAATGCATTC+AGG 0.326547 8.1:+15480831 None:intergenic
CACAGGCTGGGATTAAGTTA+TGG 0.366620 8.1:-15480809 MS.gene80557:CDS
TGGAGACAGCAATTAATATT+GGG 0.418327 8.1:-15480767 MS.gene80557:intron
ACAGGCTGGGATTAAGTTAT+GGG 0.432432 8.1:-15480808 MS.gene80557:CDS
AGTGTCATGTTTGAACTACT+TGG 0.440058 8.1:-15481236 MS.gene80557:CDS
ATTAAGTTATGGGTATTGAC+CGG 0.516370 8.1:-15480798 MS.gene80557:CDS
TTGAAGATAAACTACAAGAC+GGG 0.516777 8.1:-15480979 MS.gene80557:CDS
ATTAATGAATATGCTGACTC+TGG 0.524118 8.1:-15481172 MS.gene80557:CDS
AATATGCTGACTCTGGTTTG+AGG 0.527796 8.1:-15481165 MS.gene80557:CDS
ATTGACAAACTAGCACAGGC+TGG 0.548641 8.1:-15480822 MS.gene80557:CDS
TTGACAAACTAGCACAGGCT+GGG 0.579621 8.1:-15480821 MS.gene80557:CDS
GCTTGTAGTTTGCTTAGACA+AGG 0.583824 8.1:-15480652 MS.gene80557:CDS
TTAAGTTATGGGTATTGACC+GGG 0.584387 8.1:-15480797 MS.gene80557:CDS
CATATTGCAAAACATTGAGA+AGG 0.586625 8.1:-15481029 MS.gene80557:CDS
ATGCATTGACAAACTAGCAC+AGG 0.590563 8.1:-15480826 MS.gene80557:CDS
TTCCTCATCGAGTTCACGAT+AGG 0.605431 8.1:+15481129 None:intergenic
CGCCTATCGTGAACTCGATG+AGG 0.615127 8.1:-15481131 MS.gene80557:CDS
GTTGAAGATAAACTACAAGA+CGG 0.620454 8.1:-15480980 MS.gene80557:CDS
TTGTAGTTTATCTTCAACTG+CGG 0.627689 8.1:+15480985 None:intergenic
AGATCAAGAGCAGATTGTAG+AGG 0.657379 8.1:-15481053 MS.gene80557:CDS
TAAGTTATGGGTATTGACCG+GGG 0.665514 8.1:-15480796 MS.gene80557:CDS
TGAAGATAAACTACAAGACG+GGG 0.696798 8.1:-15480978 MS.gene80557:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TTGAACTACTTGGAAAAAAA+TGG - chr8.1:15480638-15480657 MS.gene80557:CDS 25.0%
!! ATAATAACATGTCACTGATT+AGG - chr8.1:15481165-15481184 MS.gene80557:CDS 25.0%
!!! AATAGTTCTATGTGTTGTTT+AGG - chr8.1:15481008-15481027 MS.gene80557:CDS 25.0%
ACTACTTGGAAAAAAATGGA+AGG - chr8.1:15480642-15480661 MS.gene80557:CDS 30.0%
ATGGAGACAGCAATTAATAT+TGG - chr8.1:15481096-15481115 MS.gene80557:CDS 30.0%
CATATTGCAAAACATTGAGA+AGG - chr8.1:15480835-15480854 MS.gene80557:CDS 30.0%
CTACTTGGAAAAAAATGGAA+GGG - chr8.1:15480643-15480662 MS.gene80557:CDS 30.0%
GTTGAAGATAAACTACAAGA+CGG - chr8.1:15480884-15480903 MS.gene80557:intron 30.0%
TGGAGACAGCAATTAATATT+GGG - chr8.1:15481097-15481116 MS.gene80557:CDS 30.0%
TTGAAGATAAACTACAAGAC+GGG - chr8.1:15480885-15480904 MS.gene80557:intron 30.0%
TTGTAGTTTATCTTCAACTG+CGG + chr8.1:15480882-15480901 None:intergenic 30.0%
! CTGCTTGAAAAATTTGAAAC+CGG + chr8.1:15480925-15480944 None:intergenic 30.0%
!! ATTAAGTTATGGGTATTGAC+CGG - chr8.1:15481066-15481085 MS.gene80557:CDS 30.0%
!! ATTAATGAATATGCTGACTC+TGG - chr8.1:15480692-15480711 MS.gene80557:intron 30.0%
ACGGGGTATGTTATAAATAC+CGG - chr8.1:15480903-15480922 MS.gene80557:intron 35.0%
GAGAAGGATTTGATTCTTCT+TGG - chr8.1:15480851-15480870 MS.gene80557:intron 35.0%
TGAAGATAAACTACAAGACG+GGG - chr8.1:15480886-15480905 MS.gene80557:intron 35.0%
!! TTAAGTTATGGGTATTGACC+GGG - chr8.1:15481067-15481086 MS.gene80557:CDS 35.0%
ACAGGCTGGGATTAAGTTAT+GGG - chr8.1:15481056-15481075 MS.gene80557:CDS 40.0%
AGATCAAGAGCAGATTGTAG+AGG - chr8.1:15480811-15480830 MS.gene80557:CDS 40.0%
! ATGCATTGACAAACTAGCAC+AGG - chr8.1:15481038-15481057 MS.gene80557:CDS 40.0%
! GCTAGTTTGTCAATGCATTC+AGG + chr8.1:15481036-15481055 None:intergenic 40.0%
!! AATATGCTGACTCTGGTTTG+AGG - chr8.1:15480699-15480718 MS.gene80557:intron 40.0%
!! GCTTGTAGTTTGCTTAGACA+AGG - chr8.1:15481212-15481231 MS.gene80557:CDS 40.0%
!! TAAGTTATGGGTATTGACCG+GGG - chr8.1:15481068-15481087 MS.gene80557:CDS 40.0%
ACAAGCAAATCTGCAGTCAC+AGG + chr8.1:15481198-15481217 None:intergenic 45.0%
ATTGACAAACTAGCACAGGC+TGG - chr8.1:15481042-15481061 MS.gene80557:CDS 45.0%
CACAGGCTGGGATTAAGTTA+TGG - chr8.1:15481055-15481074 MS.gene80557:CDS 45.0%
TTCCTCATCGAGTTCACGAT+AGG + chr8.1:15480738-15480757 None:intergenic 45.0%
TTGACAAACTAGCACAGGCT+GGG - chr8.1:15481043-15481062 MS.gene80557:CDS 45.0%
! TGCTGTCTCCATTTTATCCC+CGG + chr8.1:15481088-15481107 None:intergenic 45.0%
!! GGTATTGACCGGGGATAAAA+TGG - chr8.1:15481077-15481096 MS.gene80557:CDS 45.0%
CGCCTATCGTGAACTCGATG+AGG - chr8.1:15480733-15480752 MS.gene80557:intron 55.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 15480630 15481256 15480630 ID=MS.gene80557
chr8.1 mRNA 15480630 15481256 15480630 ID=MS.gene80557.t1;Parent=MS.gene80557
chr8.1 exon 15480979 15481256 15480979 ID=MS.gene80557.t1.exon1;Parent=MS.gene80557.t1
chr8.1 CDS 15480979 15481256 15480979 ID=cds.MS.gene80557.t1;Parent=MS.gene80557.t1
chr8.1 exon 15480768 15480856 15480768 ID=MS.gene80557.t1.exon2;Parent=MS.gene80557.t1
chr8.1 CDS 15480768 15480856 15480768 ID=cds.MS.gene80557.t1;Parent=MS.gene80557.t1
chr8.1 exon 15480630 15480678 15480630 ID=MS.gene80557.t1.exon3;Parent=MS.gene80557.t1
chr8.1 CDS 15480630 15480678 15480630 ID=cds.MS.gene80557.t1;Parent=MS.gene80557.t1
Gene Sequence

>MS.gene80557

TGTCATGTTTGAACTACTTGGAAAAAAATGGAAGGGAGTTTGAAGAGCAGACTAAGTATCACATTAATGAATATGCTGACTCTGGTTTGAGGACTTTGATACTCGCCTATCGTGAACTCGATGAGGAAGAGTACAAGCAATTTAATAAAGAGTTGACAGACGCTAAAAACTTAGTTAGTGCAGATCAAGAGCAGATTGTAGAGGACATATTGCAAAACATTGAGAAGGATTTGATTCTTCTTGGTGCTACCGCAGTTGAAGATAAACTACAAGACGGGGTATGTTATAAATACCGGTTTCAAATTTTTCAAGCAGTTATTTATTTATTATGAATTTTGATATTAGCATTGTTATATTATATTGTAAAAAACTTGTGCTAATAGTTCTATGTGTTGTTTAGGTTCCTGAATGCATTGACAAACTAGCACAGGCTGGGATTAAGTTATGGGTATTGACCGGGGATAAAATGGAGACAGCAATTAATATTGGGTAAGTTTAAAGATTATATACATAATTTTTGATATTGTATTTGCAAATAATAACATGTCACTGATTAGGTTATTTTCCTGTGACTGCAGATTTGCTTGTAGTTTGCTTAGACAAGGAATGAAACAAATTATCATCTAA

Protein sequence

>MS.gene80557.t1

CHVUTTWKKMEGSLKSRLSITLMNMLTLVUGLUYSPIVNSMRKSTSNLIKSUQTLKTULVQIKSRLURTYCKTLRRIUFFLVLPQLKINYKTGFLNALTNUHRLGLSYGYUPGIKWRQQLILDLLVVCLDKEUNKLSS