AlfalfaGEDB | MS.gene54912

M. sativa cultivar XinJiangDaYe / MS.gene54912

KEGG: K02925
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0019538 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576
PFAM: PF00297.25

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene54912.t1	XP_024642001.1	97.2	389	11	0	1	389	1	389	7.60E-218	766.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene54912.t1	P17094	86.0	387	54	0	1	387	1	387	1.7e-197	689.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene54912.t1	G7IBY1	97.2	389	11	0	1	389	1	389	5.5e-218	766.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene060432	MS.gene54912	0.839846	1.24E-57	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene54912	MS.gene028300	PPI
MS.gene54912	MS.gene072064	PPI
MS.gene54912	MS.gene96312	PPI
MS.gene54912	MS.gene40020	PPI
MS.gene54912	MS.gene047421	PPI
MS.gene92970	MS.gene54912	PPI
MS.gene54912	MS.gene70551	PPI
MS.gene54912	MS.gene047734	PPI
MS.gene54912	MS.gene061905	PPI
MS.gene059846	MS.gene54912	PPI
MS.gene54912	MS.gene000480	PPI
MS.gene54912	MS.gene067072	PPI
MS.gene54912	MS.gene018651	PPI
MS.gene54912	MS.gene029415	PPI
MS.gene54912	MS.gene59319	PPI
MS.gene54912	MS.gene57660	PPI
MS.gene54912	MS.gene90360	PPI
MS.gene54912	MS.gene065189	PPI
MS.gene54912	MS.gene95472	PPI
MS.gene54912	MS.gene32091	PPI
MS.gene54912	MS.gene75537	PPI
MS.gene54912	MS.gene39294	PPI
MS.gene54912	MS.gene95471	PPI
MS.gene069038	MS.gene54912	PPI
MS.gene54912	MS.gene065146	PPI
MS.gene54912	MS.gene010453	PPI
MS.gene54912	MS.gene01172	PPI
MS.gene54912	MS.gene004704	PPI
MS.gene95644	MS.gene54912	PPI
MS.gene54912	MS.gene77392	PPI
MS.gene54912	MS.gene96780	PPI
MS.gene54912	MS.gene45646	PPI
MS.gene54912	MS.gene56525	PPI
MS.gene018553	MS.gene54912	PPI
MS.gene54912	MS.gene09845	PPI
MS.gene54912	MS.gene40019	PPI
MS.gene54912	MS.gene36414	PPI
MS.gene54912	MS.gene031484	PPI
MS.gene54912	MS.gene022752	PPI
MS.gene28749	MS.gene54912	PPI
MS.gene54912	MS.gene045180	PPI
MS.gene006800	MS.gene54912	PPI
MS.gene54912	MS.gene031406	PPI
MS.gene54912	MS.gene065652	PPI
MS.gene54912	MS.gene071916	PPI
MS.gene54912	MS.gene028377	PPI
MS.gene54912	MS.gene96949	PPI
MS.gene54912	MS.gene020215	PPI
MS.gene54912	MS.gene43350	PPI
MS.gene068781	MS.gene54912	PPI
MS.gene073324	MS.gene54912	PPI
MS.gene54912	MS.gene023154	PPI
MS.gene54912	MS.gene002204	PPI
MS.gene54912	MS.gene004705	PPI
MS.gene54912	MS.gene35157	PPI
MS.gene54912	MS.gene037902	PPI
MS.gene54912	MS.gene41329	PPI
MS.gene54912	MS.gene035194	PPI
MS.gene54912	MS.gene059878	PPI
MS.gene53720	MS.gene54912	PPI
MS.gene54912	MS.gene23437	PPI
MS.gene54912	MS.gene041557	PPI
MS.gene54912	MS.gene64512	PPI
MS.gene54912	MS.gene002038	PPI
MS.gene54912	MS.gene36492	PPI
MS.gene54912	MS.gene012331	PPI
MS.gene54912	MS.gene060923	PPI
MS.gene54912	MS.gene047206	PPI
MS.gene54912	MS.gene036487	PPI
MS.gene54912	MS.gene031378	PPI
MS.gene54912	MS.gene012333	PPI
MS.gene54912	MS.gene029547	PPI
MS.gene54912	MS.gene035331	PPI
MS.gene54912	MS.gene32092	PPI
MS.gene54912	MS.gene059880	PPI
MS.gene54912	MS.gene028376	PPI
MS.gene35076	MS.gene54912	PPI
MS.gene54912	MS.gene00612	PPI
MS.gene54912	MS.gene99779	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene54912.t1	MTR_1g098540	97.172	389	11	0	1	389	1	389	0.0	778
MS.gene54912.t1	MTR_4g094398	88.432	389	45	0	1	389	1	389	0.0	707

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	bit score
MS.gene54912.t1	AT1G43170	86.047	387	54	1	387	1	387	686
MS.gene54912.t1	AT1G43170	86.047	387	54	1	387	1	387	686
MS.gene54912.t1	AT1G43170	86.047	387	54	1	387	1	387	686
MS.gene54912.t1	AT1G43170	86.047	387	54	1	387	1	387	686
MS.gene54912.t1	AT1G43170	86.047	387	54	1	387	1	387	686
MS.gene54912.t1	AT1G43170	86.047	387	54	1	387	1	387	686
MS.gene54912.t1	AT1G43170	86.047	387	54	1	387	1	387	686
MS.gene54912.t1	AT1G43170	86.047	387	54	1	387	1	387	686
MS.gene54912.t1	AT1G61580	83.938	386	62	1	386	1	386	674
MS.gene54912.t1	AT1G43170	84.354	294	46	94	387	11	304	531

Find 126 sgRNAs with CRISPR-Local

Find 129 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TAACTTGAATTTCCATGATA+TGG	0.153600	4.4:-66269321	None:intergenic
CGTCAATCATTTCATCCTTC+TGG	0.256903	4.4:-66269420	None:intergenic
GGCTTGGTTGGGTCATCCTT+AGG	0.263169	4.4:-66268881	None:intergenic
ATCGATACCTCTTCCAAGTT+TGG	0.285033	4.4:+66269871	MS.gene54912:CDS
ACCCAAGCTAACTGCCTTCT+TGG	0.286054	4.4:+66268904	MS.gene54912:CDS
GCACCCAAGACACGGTTCTT+TGG	0.287363	4.4:+66268805	MS.gene54912:CDS
CACCCAAGACACGGTTCTTT+GGG	0.292688	4.4:+66268806	MS.gene54912:CDS
TGACGAGCAGCACGCTTTCT+TGG	0.308401	4.4:-66268836	None:intergenic
GTTAGCTTGGGTGGCTTGGT+TGG	0.310280	4.4:-66268893	None:intergenic
TCAGAGAAGCAAAAGTTCTA+TGG	0.364679	4.4:+66269916	MS.gene54912:CDS
TTCTTGTGAAGCTTGGATCC+TGG	0.364837	4.4:-66268971	None:intergenic
AACTTGAATTTCCATGATAT+GGG	0.365910	4.4:-66269320	None:intergenic
TTAGCTTGGGTGGCTTGGTT+GGG	0.372233	4.4:-66268892	None:intergenic
GGCAGTTAGCTTGGGTGGCT+TGG	0.374534	4.4:-66268897	None:intergenic
ACTTCTTCAGTTTCTCAAGC+TGG	0.375617	4.4:-66269228	None:intergenic
CAGAGTAATAACCCTCTTCT+TGG	0.375680	4.4:-66269788	None:intergenic
TTTGCTTCTCTGATGTTGTC+TGG	0.376238	4.4:-66269906	None:intergenic
GCTTGGTTGGGTCATCCTTA+GGG	0.388678	4.4:-66268880	None:intergenic
CTTCTTCAGTTTCTCAAGCT+GGG	0.407032	4.4:-66269227	None:intergenic
TTGAGGAAGGTTGCATGTAT+TGG	0.407927	4.4:+66269529	MS.gene54912:CDS
GACGAGCAGCACGCTTTCTT+GGG	0.410830	4.4:-66268835	None:intergenic
AACTGCCTTCTTGGGTTACA+AGG	0.417572	4.4:+66268913	MS.gene54912:CDS
ATCATGGAAATTCAAGTTAA+CGG	0.420034	4.4:+66269325	MS.gene54912:CDS
TCTTTCACTGTTGCAAGAGC+TGG	0.422143	4.4:+66269574	MS.gene54912:CDS
TCCGAACATTGAACACTGTC+TGG	0.427828	4.4:+66269080	MS.gene54912:CDS
TACCATATAATTAAGCTTTG+AGG	0.428051	4.4:-66269942	None:intergenic
CCCAAGCTAACTGCCTTCTT+GGG	0.428836	4.4:+66268905	MS.gene54912:CDS
ATTTACAAGCTCGGCAAAGT+TGG	0.433680	4.4:+66269637	MS.gene54912:CDS
ACCCAAGAAGGCAGTTAGCT+TGG	0.433816	4.4:-66268906	None:intergenic
ATCCCAAAGAACCGTGTCTT+GGG	0.433824	4.4:-66268808	None:intergenic
ATGAAGGTGTTGTTACTCGT+TGG	0.437276	4.4:+66269470	MS.gene54912:CDS
ATGGTTGTTGTCGGTGTTGT+GGG	0.440477	4.4:+66269034	MS.gene54912:CDS
GAAACGACCATGTCCAAACT+TGG	0.447320	4.4:-66269884	None:intergenic
AGTCTCCTTCTTGTGAAGCT+TGG	0.450717	4.4:-66268978	None:intergenic
CATTGGAGTGACCAAGGGTA+AGG	0.450805	4.4:+66269444	MS.gene54912:CDS
GATGAAATGATTGACGTCAT+TGG	0.450828	4.4:+66269427	MS.gene54912:CDS
GCCTTCTTGGGTTACAAGGC+TGG	0.450941	4.4:+66268917	MS.gene54912:CDS
TGAAGGTGTTGTTACTCGTT+GGG	0.451002	4.4:+66269471	MS.gene54912:CDS
ATACAGTTTCTTTGAGAAGC+AGG	0.454598	4.4:+66269378	MS.gene54912:CDS
CGAACTCAGTCTGGCCGCCA+TGG	0.455821	4.4:-66269669	None:intergenic
GTCTTCCTCGTAAGACTCAC+AGG	0.464950	4.4:+66269503	MS.gene54912:CDS
TGAGAAAGACATTACACCTA+TGG	0.467448	4.4:+66269699	MS.gene54912:CDS
GTAAGACTCACAGGGGTTTG+AGG	0.468820	4.4:+66269512	MS.gene54912:CDS
CCCAAGAAGGCAGTTAGCTT+GGG	0.469247	4.4:-66268905	None:intergenic
CAGTCCTATCGAACTCAGTC+TGG	0.471066	4.4:-66269678	None:intergenic
TCCAGACAGTGTTCAATGTT+CGG	0.473376	4.4:-66269081	None:intergenic
GACTTCTCGTCTTGCTCTTG+AGG	0.480840	4.4:+66269831	MS.gene54912:CDS
TATGGTTGTTGTCGGTGTTG+TGG	0.481177	4.4:+66269033	MS.gene54912:CDS
TGTTGGCTCACACTCAGATC+AGG	0.484616	4.4:+66269269	MS.gene54912:CDS
CACTCAGATCAGGAAAATGA+AGG	0.485840	4.4:+66269279	MS.gene54912:CDS
TGTTCAGCTCAGTTCTGTGA+TGG	0.485951	4.4:-66269609	None:intergenic
AATCCCAAAGAACCGTGTCT+TGG	0.489699	4.4:-66268809	None:intergenic
AACAAGAAAATTTACAAGCT+CGG	0.491496	4.4:+66269628	MS.gene54912:CDS
AGAGTAATAACCCTCTTCTT+GGG	0.492275	4.4:-66269787	None:intergenic
GACGAGAAGTCTGCTTAAGA+AGG	0.502167	4.4:-66269819	None:intergenic
ACCCCTCCTATGGTTGTTGT+CGG	0.504742	4.4:+66269025	MS.gene54912:CDS
GCAGAAGAAAGCCCATATCA+TGG	0.507648	4.4:+66269309	MS.gene54912:CDS
GGAAGAAGAAGATGTCTCAC+AGG	0.511229	4.4:+66268774	None:intergenic
GTCAGAGAAGTCGAGAAGCC+AGG	0.515408	4.4:+66268953	MS.gene54912:CDS
ACCTTCATAACCCTTACCCT+TGG	0.517932	4.4:-66269455	None:intergenic
AATCATTGAGACCCCTCCTA+TGG	0.520054	4.4:+66269015	MS.gene54912:CDS
ATGATCAAGGGAGGCTGTGT+TGG	0.526401	4.4:+66269763	MS.gene54912:CDS
ACCAAGGGTAAGGGTTATGA+AGG	0.530593	4.4:+66269454	MS.gene54912:CDS
GAACTCAGTCTGGCCGCCAT+GGG	0.538841	4.4:-66269668	None:intergenic
GTTCAATGTTCGGAGACCAC+GGG	0.540332	4.4:-66269071	None:intergenic
AAGCAGTATAAAAGTGATGA+GGG	0.545940	4.4:+66269187	MS.gene54912:CDS
ACCAGCCTTGTAACCCAAGA+AGG	0.552644	4.4:-66268918	None:intergenic
TATACTGCTTTGAGTAATTG+CGG	0.553884	4.4:-66269174	None:intergenic
CGGTGGTACCATCGCACAGA+AGG	0.555485	4.4:+66269345	MS.gene54912:CDS
AGGATCCAAGCTTCACAAGA+AGG	0.558613	4.4:+66268973	MS.gene54912:CDS
AAGAAGGCAGTTAGCTTGGG+TGG	0.559172	4.4:-66268902	None:intergenic
ACACCGACAACAACCATAGG+AGG	0.565736	4.4:-66269028	None:intergenic
GACTCACAGGGGTTTGAGGA+AGG	0.568790	4.4:+66269516	MS.gene54912:CDS
AAAGCAGTATAAAAGTGATG+AGG	0.570008	4.4:+66269186	MS.gene54912:CDS
AAGTCTGCTTAAGAAGGGAC+TGG	0.577488	4.4:-66269813	None:intergenic
TGTTCAATGTTCGGAGACCA+CGG	0.577689	4.4:-66269072	None:intergenic
AAAAGTGAAGGCTTTCCCTA+AGG	0.579121	4.4:+66268865	MS.gene54912:CDS
ACCTCTTCCAAGTTTGGACA+TGG	0.582976	4.4:+66269877	MS.gene54912:CDS
TCTTCCTCGTAAGACTCACA+GGG	0.584859	4.4:+66269504	MS.gene54912:CDS
ACAACACCGACAACAACCAT+AGG	0.591726	4.4:-66269031	None:intergenic
ATTGGAGTGACCAAGGGTAA+GGG	0.593223	4.4:+66269445	MS.gene54912:CDS
GCGGCCAGACTGAGTTCGAT+AGG	0.593362	4.4:+66269674	MS.gene54912:CDS
TGGTACCATCGCACAGAAGG+TGG	0.595874	4.4:+66269348	MS.gene54912:CDS
GAGAAAGACATTACACCTAT+GGG	0.596080	4.4:+66269700	MS.gene54912:CDS
TGACGTCATTGGAGTGACCA+AGG	0.598559	4.4:+66269438	MS.gene54912:CDS
AGGTTGCATGTATTGGTGCT+TGG	0.600568	4.4:+66269536	MS.gene54912:CDS
ACCATGTCCAAACTTGGAAG+AGG	0.600841	4.4:-66269878	None:intergenic
ACAGTGAAAGAAACTCTAGC+AGG	0.607261	4.4:-66269562	None:intergenic
CACCGACAACAACCATAGGA+GGG	0.607553	4.4:-66269027	None:intergenic
ATGGGTGGTTTCCCACACTA+CGG	0.607609	4.4:+66269718	MS.gene54912:CDS
CTGTGTTGGTCCCAAGAAGA+GGG	0.610053	4.4:+66269777	MS.gene54912:CDS
TGCAACTGTTATCCGTGTGT+TGG	0.610763	4.4:+66269252	MS.gene54912:CDS
TATCGATGCTGTGTTCCAGA+AGG	0.612569	4.4:+66269405	MS.gene54912:CDS
ACGAGAAGTCTGCTTAAGAA+GGG	0.612938	4.4:-66269818	None:intergenic
GCTGTGTTGGTCCCAAGAAG+AGG	0.613945	4.4:+66269776	MS.gene54912:CDS
AAGTTCGAGCACCCAAGACA+CGG	0.616675	4.4:+66268797	MS.gene54912:CDS
CCTTGACAATACCGTAGTGT+GGG	0.616873	4.4:-66269729	None:intergenic
GTTGCAAGAGCTGGTCAGAA+CGG	0.617265	4.4:+66269583	MS.gene54912:CDS
CTGGTGCAAGTCCATGCAGA+AGG	0.618228	4.4:+66269147	MS.gene54912:CDS
CAAGCTCGGCAAAGTTGGAG+AGG	0.619055	4.4:+66269642	MS.gene54912:CDS
CCCACACTACGGTATTGTCA+AGG	0.620478	4.4:+66269729	MS.gene54912:CDS
GAAGGTGTTGTTACTCGTTG+GGG	0.623077	4.4:+66269472	MS.gene54912:CDS
TTCTGGAACACAGCATCGAT+AGG	0.625976	4.4:-66269403	None:intergenic
TAGTGTGGGAAACCACCCAT+AGG	0.626093	4.4:-66269715	None:intergenic
GACGTCATTGGAGTGACCAA+GGG	0.628824	4.4:+66269439	MS.gene54912:CDS
ATTGCGGAAAGCCTTCTGCA+TGG	0.629972	4.4:-66269158	None:intergenic
AAAGTCCACCTTCTGTGCGA+TGG	0.631954	4.4:-66269353	None:intergenic
ACTCAGATCAGGAAAATGAA+GGG	0.633414	4.4:+66269280	MS.gene54912:CDS
AAAGTTGGAGAGGAGACCCA+TGG	0.636220	4.4:+66269652	MS.gene54912:CDS
ACCGACAACAACCATAGGAG+GGG	0.637572	4.4:-66269026	None:intergenic
CTGGACTCAGCATTTGAGTG+AGG	0.638592	4.4:+66269099	MS.gene54912:CDS
AGGGGTCTCAATGATTGTCA+CGG	0.647331	4.4:-66269008	None:intergenic
TCCTTGACAATACCGTAGTG+TGG	0.648748	4.4:-66269730	None:intergenic
AAAGACATTACACCTATGGG+TGG	0.665025	4.4:+66269703	MS.gene54912:CDS
TCGTCACAGAGGAAAAGTGA+AGG	0.670298	4.4:+66268853	MS.gene54912:CDS
AAACCCCTGTGAGTCTTACG+AGG	0.675290	4.4:-66269508	None:intergenic
TCTGAGTGTGAGCCAACACA+CGG	0.681973	4.4:-66269264	None:intergenic
GGCTATGTGAAAACTCCCCG+TGG	0.687655	4.4:+66269055	MS.gene54912:CDS
AGCAGTATAAAAGTGATGAG+GGG	0.688880	4.4:+66269188	MS.gene54912:CDS
TTCAATGTTCGGAGACCACG+GGG	0.689564	4.4:-66269070	None:intergenic
TGAGTGAGGAAATTAAGCGT+CGG	0.691049	4.4:+66269113	MS.gene54912:CDS
AAGCGTGCTGCTCGTCACAG+AGG	0.692287	4.4:+66268842	MS.gene54912:CDS
ACACTACGGTATTGTCAAGG+AGG	0.722752	4.4:+66269732	MS.gene54912:CDS
ATGGAAATTCAAGTTAACGG+TGG	0.728022	4.4:+66269328	MS.gene54912:CDS
CTTCCTCGTAAGACTCACAG+GGG	0.755519	4.4:+66269505	MS.gene54912:CDS
GTTGGAGAGGAGACCCATGG+CGG	0.788297	4.4:+66269655	MS.gene54912:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AACAAGAAAATTTACAAGCT+CGG	+	chr4.4:66269628-66269647	MS.gene54912:CDS	25.0%
!	AACTTGAATTTCCATGATAT+GGG	-	chr4.4:66269323-66269342	None:intergenic	25.0%
!	TAACTTGAATTTCCATGATA+TGG	-	chr4.4:66269324-66269343	None:intergenic	25.0%
!!	ATCATGGAAATTCAAGTTAA+CGG	+	chr4.4:66269325-66269344	MS.gene54912:CDS	25.0%
	AAAGCAGTATAAAAGTGATG+AGG	+	chr4.4:66269186-66269205	MS.gene54912:CDS	30.0%
	AAGCAGTATAAAAGTGATGA+GGG	+	chr4.4:66269187-66269206	MS.gene54912:CDS	30.0%
!	TATACTGCTTTGAGTAATTG+CGG	-	chr4.4:66269177-66269196	None:intergenic	30.0%
	ACTCAGATCAGGAAAATGAA+GGG	+	chr4.4:66269280-66269299	MS.gene54912:CDS	35.0%
	AGAGTAATAACCCTCTTCTT+GGG	-	chr4.4:66269790-66269809	None:intergenic	35.0%
	AGCAGTATAAAAGTGATGAG+GGG	+	chr4.4:66269188-66269207	MS.gene54912:CDS	35.0%
	GAGAAAGACATTACACCTAT+GGG	+	chr4.4:66269700-66269719	MS.gene54912:CDS	35.0%
	GATGAAATGATTGACGTCAT+TGG	+	chr4.4:66269427-66269446	MS.gene54912:CDS	35.0%
	TCAGAGAAGCAAAAGTTCTA+TGG	+	chr4.4:66269916-66269935	MS.gene54912:CDS	35.0%
	TGAGAAAGACATTACACCTA+TGG	+	chr4.4:66269699-66269718	MS.gene54912:CDS	35.0%
!	ATACAGTTTCTTTGAGAAGC+AGG	+	chr4.4:66269378-66269397	MS.gene54912:CDS	35.0%
!	ATGGAAATTCAAGTTAACGG+TGG	+	chr4.4:66269328-66269347	MS.gene54912:CDS	35.0%
!	GAGGATTTTCTGATGATCAA+GGG	+	chr4.4:66269751-66269770	MS.gene54912:CDS	35.0%
!!!	AATTAAGCGTCGGTTTTACA+CGG	+	chr4.4:66269123-66269142	MS.gene54912:CDS	35.0%
	AAAAGTGAAGGCTTTCCCTA+AGG	+	chr4.4:66268865-66268884	MS.gene54912:CDS	40.0%
	AAAGACATTACACCTATGGG+TGG	+	chr4.4:66269703-66269722	MS.gene54912:CDS	40.0%
	ACAGTGAAAGAAACTCTAGC+AGG	-	chr4.4:66269565-66269584	None:intergenic	40.0%
	ACGAGAAGTCTGCTTAAGAA+GGG	-	chr4.4:66269821-66269840	None:intergenic	40.0%
	ACTTCTTCAGTTTCTCAAGC+TGG	-	chr4.4:66269231-66269250	None:intergenic	40.0%
	ATCGATACCTCTTCCAAGTT+TGG	+	chr4.4:66269871-66269890	MS.gene54912:CDS	40.0%
	ATTTACAAGCTCGGCAAAGT+TGG	+	chr4.4:66269637-66269656	MS.gene54912:CDS	40.0%
	CACTCAGATCAGGAAAATGA+AGG	+	chr4.4:66269279-66269298	MS.gene54912:CDS	40.0%
	CAGAGTAATAACCCTCTTCT+TGG	-	chr4.4:66269791-66269810	None:intergenic	40.0%
	CGTCAATCATTTCATCCTTC+TGG	-	chr4.4:66269423-66269442	None:intergenic	40.0%
	CTTCTTCAGTTTCTCAAGCT+GGG	-	chr4.4:66269230-66269249	None:intergenic	40.0%
	TCCAGACAGTGTTCAATGTT+CGG	-	chr4.4:66269084-66269103	None:intergenic	40.0%
	TGAGTGAGGAAATTAAGCGT+CGG	+	chr4.4:66269113-66269132	MS.gene54912:CDS	40.0%
	TTGAGGAAGGTTGCATGTAT+TGG	+	chr4.4:66269529-66269548	MS.gene54912:CDS	40.0%
	TTTGCTTCTCTGATGTTGTC+TGG	-	chr4.4:66269909-66269928	None:intergenic	40.0%
!	GATTTTCTGATGATCAAGGG+AGG	+	chr4.4:66269754-66269773	MS.gene54912:CDS	40.0%
!	GGAGGATTTTCTGATGATCA+AGG	+	chr4.4:66269750-66269769	MS.gene54912:CDS	40.0%
!!	ATGAAGGTGTTGTTACTCGT+TGG	+	chr4.4:66269470-66269489	MS.gene54912:CDS	40.0%
!!	TGAAGGTGTTGTTACTCGTT+GGG	+	chr4.4:66269471-66269490	MS.gene54912:CDS	40.0%
	AACTGCCTTCTTGGGTTACA+AGG	+	chr4.4:66268913-66268932	MS.gene54912:CDS	45.0%
	AAGTCTGCTTAAGAAGGGAC+TGG	-	chr4.4:66269816-66269835	None:intergenic	45.0%
	AATCATTGAGACCCCTCCTA+TGG	+	chr4.4:66269015-66269034	MS.gene54912:CDS	45.0%
	AATCCCAAAGAACCGTGTCT+TGG	-	chr4.4:66268812-66268831	None:intergenic	45.0%
	ACAACACCGACAACAACCAT+AGG	-	chr4.4:66269034-66269053	None:intergenic	45.0%
	ACACTACGGTATTGTCAAGG+AGG	+	chr4.4:66269732-66269751	MS.gene54912:CDS	45.0%
	ACCAAGGGTAAGGGTTATGA+AGG	+	chr4.4:66269454-66269473	MS.gene54912:CDS	45.0%
	ACCATGTCCAAACTTGGAAG+AGG	-	chr4.4:66269881-66269900	None:intergenic	45.0%
	ACCTCTTCCAAGTTTGGACA+TGG	+	chr4.4:66269877-66269896	MS.gene54912:CDS	45.0%
	ACCTTCATAACCCTTACCCT+TGG	-	chr4.4:66269458-66269477	None:intergenic	45.0%
	AGGATCCAAGCTTCACAAGA+AGG	+	chr4.4:66268973-66268992	MS.gene54912:CDS	45.0%
	AGGGGTCTCAATGATTGTCA+CGG	-	chr4.4:66269011-66269030	None:intergenic	45.0%
	AGTCTCCTTCTTGTGAAGCT+TGG	-	chr4.4:66268981-66269000	None:intergenic	45.0%
	ATCCCAAAGAACCGTGTCTT+GGG	-	chr4.4:66268811-66268830	None:intergenic	45.0%
	ATTGGAGTGACCAAGGGTAA+GGG	+	chr4.4:66269445-66269464	MS.gene54912:CDS	45.0%
	CCTTGACAATACCGTAGTGT+GGG	-	chr4.4:66269732-66269751	None:intergenic	45.0%
	GAAACGACCATGTCCAAACT+TGG	-	chr4.4:66269887-66269906	None:intergenic	45.0%
	GACGAGAAGTCTGCTTAAGA+AGG	-	chr4.4:66269822-66269841	None:intergenic	45.0%
	GCAGAAGAAAGCCCATATCA+TGG	+	chr4.4:66269309-66269328	MS.gene54912:CDS	45.0%
	TATCGATGCTGTGTTCCAGA+AGG	+	chr4.4:66269405-66269424	MS.gene54912:CDS	45.0%
	TCCGAACATTGAACACTGTC+TGG	+	chr4.4:66269080-66269099	MS.gene54912:CDS	45.0%
	TCCTTGACAATACCGTAGTG+TGG	-	chr4.4:66269733-66269752	None:intergenic	45.0%
	TCGTCACAGAGGAAAAGTGA+AGG	+	chr4.4:66268853-66268872	MS.gene54912:CDS	45.0%
	TCTTCCTCGTAAGACTCACA+GGG	+	chr4.4:66269504-66269523	MS.gene54912:CDS	45.0%
	TCTTTCACTGTTGCAAGAGC+TGG	+	chr4.4:66269574-66269593	MS.gene54912:CDS	45.0%
	TGCAACTGTTATCCGTGTGT+TGG	+	chr4.4:66269252-66269271	MS.gene54912:CDS	45.0%
	TGTTCAATGTTCGGAGACCA+CGG	-	chr4.4:66269075-66269094	None:intergenic	45.0%
	TGTTCAGCTCAGTTCTGTGA+TGG	-	chr4.4:66269612-66269631	None:intergenic	45.0%
	TTCTTGTGAAGCTTGGATCC+TGG	-	chr4.4:66268974-66268993	None:intergenic	45.0%
!	AGGTTGCATGTATTGGTGCT+TGG	+	chr4.4:66269536-66269555	MS.gene54912:CDS	45.0%
!!	ATGGTTGTTGTCGGTGTTGT+GGG	+	chr4.4:66269034-66269053	MS.gene54912:CDS	45.0%
!!	GAAGGTGTTGTTACTCGTTG+GGG	+	chr4.4:66269472-66269491	MS.gene54912:CDS	45.0%
!!	TATGGTTGTTGTCGGTGTTG+TGG	+	chr4.4:66269033-66269052	MS.gene54912:CDS	45.0%
!!	TTCTGGAACACAGCATCGAT+AGG	-	chr4.4:66269406-66269425	None:intergenic	45.0%
	AAACCCCTGTGAGTCTTACG+AGG	-	chr4.4:66269511-66269530	None:intergenic	50.0%
	AAAGTCCACCTTCTGTGCGA+TGG	-	chr4.4:66269356-66269375	None:intergenic	50.0%
	AAAGTTGGAGAGGAGACCCA+TGG	+	chr4.4:66269652-66269671	MS.gene54912:CDS	50.0%
	AAGTTCGAGCACCCAAGACA+CGG	+	chr4.4:66268797-66268816	MS.gene54912:CDS	50.0%
	ACACCGACAACAACCATAGG+AGG	-	chr4.4:66269031-66269050	None:intergenic	50.0%
	ACCCAAGAAGGCAGTTAGCT+TGG	-	chr4.4:66268909-66268928	None:intergenic	50.0%
	ACCCAAGCTAACTGCCTTCT+TGG	+	chr4.4:66268904-66268923	MS.gene54912:CDS	50.0%
	ACCCCTCCTATGGTTGTTGT+CGG	+	chr4.4:66269025-66269044	MS.gene54912:CDS	50.0%
	ACCGACAACAACCATAGGAG+GGG	-	chr4.4:66269029-66269048	None:intergenic	50.0%
	ATGATCAAGGGAGGCTGTGT+TGG	+	chr4.4:66269763-66269782	MS.gene54912:CDS	50.0%
	ATGGGTGGTTTCCCACACTA+CGG	+	chr4.4:66269718-66269737	MS.gene54912:CDS	50.0%
	ATTGCGGAAAGCCTTCTGCA+TGG	-	chr4.4:66269161-66269180	None:intergenic	50.0%
	CACCCAAGACACGGTTCTTT+GGG	+	chr4.4:66268806-66268825	MS.gene54912:CDS	50.0%
	CACCGACAACAACCATAGGA+GGG	-	chr4.4:66269030-66269049	None:intergenic	50.0%
	CAGTCCTATCGAACTCAGTC+TGG	-	chr4.4:66269681-66269700	None:intergenic	50.0%
	CATTGGAGTGACCAAGGGTA+AGG	+	chr4.4:66269444-66269463	MS.gene54912:CDS	50.0%
	CCCAAGCTAACTGCCTTCTT+GGG	+	chr4.4:66268905-66268924	MS.gene54912:CDS	50.0%
	CCCACACTACGGTATTGTCA+AGG	+	chr4.4:66269729-66269748	MS.gene54912:CDS	50.0%
	CTGGACTCAGCATTTGAGTG+AGG	+	chr4.4:66269099-66269118	MS.gene54912:CDS	50.0%
	CTTCCTCGTAAGACTCACAG+GGG	+	chr4.4:66269505-66269524	MS.gene54912:CDS	50.0%
	GACGTCATTGGAGTGACCAA+GGG	+	chr4.4:66269439-66269458	MS.gene54912:CDS	50.0%
	GACTTCTCGTCTTGCTCTTG+AGG	+	chr4.4:66269831-66269850	MS.gene54912:CDS	50.0%
	GCTTGGTTGGGTCATCCTTA+GGG	-	chr4.4:66268883-66268902	None:intergenic	50.0%
	GTAAGACTCACAGGGGTTTG+AGG	+	chr4.4:66269512-66269531	MS.gene54912:CDS	50.0%
	GTCTTCCTCGTAAGACTCAC+AGG	+	chr4.4:66269503-66269522	MS.gene54912:CDS	50.0%
	GTTCAATGTTCGGAGACCAC+GGG	-	chr4.4:66269074-66269093	None:intergenic	50.0%
	GTTGCAAGAGCTGGTCAGAA+CGG	+	chr4.4:66269583-66269602	MS.gene54912:CDS	50.0%
	TAGTGTGGGAAACCACCCAT+AGG	-	chr4.4:66269718-66269737	None:intergenic	50.0%
	TCTGAGTGTGAGCCAACACA+CGG	-	chr4.4:66269267-66269286	None:intergenic	50.0%
	TGACGTCATTGGAGTGACCA+AGG	+	chr4.4:66269438-66269457	MS.gene54912:CDS	50.0%
	TTCAATGTTCGGAGACCACG+GGG	-	chr4.4:66269073-66269092	None:intergenic	50.0%
!	AAGAAGGCAGTTAGCTTGGG+TGG	-	chr4.4:66268905-66268924	None:intergenic	50.0%
!	ACCAGCCTTGTAACCCAAGA+AGG	-	chr4.4:66268921-66268940	None:intergenic	50.0%
!	CCCAAGAAGGCAGTTAGCTT+GGG	-	chr4.4:66268908-66268927	None:intergenic	50.0%
!	CTGTGTTGGTCCCAAGAAGA+GGG	+	chr4.4:66269777-66269796	MS.gene54912:CDS	50.0%
!!	TGTTGGCTCACACTCAGATC+AGG	+	chr4.4:66269269-66269288	MS.gene54912:CDS	50.0%
!!	TTAGCTTGGGTGGCTTGGTT+GGG	-	chr4.4:66268895-66268914	None:intergenic	50.0%
	CAAGCTCGGCAAAGTTGGAG+AGG	+	chr4.4:66269642-66269661	MS.gene54912:CDS	55.0%
	CTGGTGCAAGTCCATGCAGA+AGG	+	chr4.4:66269147-66269166	MS.gene54912:CDS	55.0%
	GACGAGCAGCACGCTTTCTT+GGG	-	chr4.4:66268838-66268857	None:intergenic	55.0%
	GACTCACAGGGGTTTGAGGA+AGG	+	chr4.4:66269516-66269535	MS.gene54912:CDS	55.0%
	GCACCCAAGACACGGTTCTT+TGG	+	chr4.4:66268805-66268824	MS.gene54912:CDS	55.0%
	GCCTTCTTGGGTTACAAGGC+TGG	+	chr4.4:66268917-66268936	MS.gene54912:CDS	55.0%
	GGCTATGTGAAAACTCCCCG+TGG	+	chr4.4:66269055-66269074	MS.gene54912:CDS	55.0%
	GTCAGAGAAGTCGAGAAGCC+AGG	+	chr4.4:66268953-66268972	MS.gene54912:CDS	55.0%
	TGACGAGCAGCACGCTTTCT+TGG	-	chr4.4:66268839-66268858	None:intergenic	55.0%
	TGGTACCATCGCACAGAAGG+TGG	+	chr4.4:66269348-66269367	MS.gene54912:CDS	55.0%
!	GCTGTGTTGGTCCCAAGAAG+AGG	+	chr4.4:66269776-66269795	MS.gene54912:CDS	55.0%
!	GGCTTGGTTGGGTCATCCTT+AGG	-	chr4.4:66268884-66268903	None:intergenic	55.0%
!!	GTTAGCTTGGGTGGCTTGGT+TGG	-	chr4.4:66268896-66268915	None:intergenic	55.0%
!!!	AGCGTCGGTTTTACACGGAC+TGG	+	chr4.4:66269128-66269147	MS.gene54912:CDS	55.0%
	CGGTGGTACCATCGCACAGA+AGG	+	chr4.4:66269345-66269364	MS.gene54912:CDS	60.0%
	GAACTCAGTCTGGCCGCCAT+GGG	-	chr4.4:66269671-66269690	None:intergenic	60.0%
	GCGGCCAGACTGAGTTCGAT+AGG	+	chr4.4:66269674-66269693	MS.gene54912:CDS	60.0%
	GTTGGAGAGGAGACCCATGG+CGG	+	chr4.4:66269655-66269674	MS.gene54912:CDS	60.0%
!	AAGCGTGCTGCTCGTCACAG+AGG	+	chr4.4:66268842-66268861	MS.gene54912:CDS	60.0%
!	GGCAGTTAGCTTGGGTGGCT+TGG	-	chr4.4:66268900-66268919	None:intergenic	60.0%
	CGAACTCAGTCTGGCCGCCA+TGG	-	chr4.4:66269672-66269691	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.4	gene	66268785	66269954	66268785	ID=MS.gene54912
chr4.4	mRNA	66268785	66269954	66268785	ID=MS.gene54912.t1;Parent=MS.gene54912
chr4.4	exon	66268785	66269954	66268785	ID=MS.gene54912.t1.exon1;Parent=MS.gene54912.t1
chr4.4	CDS	66268785	66269954	66268785	ID=cds.MS.gene54912.t1;Parent=MS.gene54912.t1

Gene Sequence

>MS.gene54912

ATGTCTCACAGGAAGTTCGAGCACCCAAGACACGGTTCTTTGGGATTTCTCCCAAGAAAGCGTGCTGCTCGTCACAGAGGAAAAGTGAAGGCTTTCCCTAAGGATGACCCAACCAAGCCACCCAAGCTAACTGCCTTCTTGGGTTACAAGGCTGGTATGACACACATTGTCAGAGAAGTCGAGAAGCCAGGATCCAAGCTTCACAAGAAGGAGACTTGTGAGTCCGTGACAATCATTGAGACCCCTCCTATGGTTGTTGTCGGTGTTGTGGGCTATGTGAAAACTCCCCGTGGTCTCCGAACATTGAACACTGTCTGGACTCAGCATTTGAGTGAGGAAATTAAGCGTCGGTTTTACACGGACTGGTGCAAGTCCATGCAGAAGGCTTTCCGCAATTACTCAAAGCAGTATAAAAGTGATGAGGGGAAGAAAAACATTCAGACCCAGCTTGAGAAACTGAAGAAGTATGCAACTGTTATCCGTGTGTTGGCTCACACTCAGATCAGGAAAATGAAGGGTTTGAAGCAGAAGAAAGCCCATATCATGGAAATTCAAGTTAACGGTGGTACCATCGCACAGAAGGTGGACTTTGCATACAGTTTCTTTGAGAAGCAGGTTCCTATCGATGCTGTGTTCCAGAAGGATGAAATGATTGACGTCATTGGAGTGACCAAGGGTAAGGGTTATGAAGGTGTTGTTACTCGTTGGGGTGTTACTCGTCTTCCTCGTAAGACTCACAGGGGTTTGAGGAAGGTTGCATGTATTGGTGCTTGGCATCCTGCTAGAGTTTCTTTCACTGTTGCAAGAGCTGGTCAGAACGGATACCATCACAGAACTGAGCTGAACAAGAAAATTTACAAGCTCGGCAAAGTTGGAGAGGAGACCCATGGCGGCCAGACTGAGTTCGATAGGACTGAGAAAGACATTACACCTATGGGTGGTTTCCCACACTACGGTATTGTCAAGGAGGATTTTCTGATGATCAAGGGAGGCTGTGTTGGTCCCAAGAAGAGGGTTATTACTCTGCGCCAGTCCCTTCTTAAGCAGACTTCTCGTCTTGCTCTTGAGGATATCAAACTCAAGTTTATCGATACCTCTTCCAAGTTTGGACATGGTCGTTTCCAGACAACATCAGAGAAGCAAAAGTTCTATGGACGCCTCAAAGCTTAA

Protein sequence

>MS.gene54912.t1

MSHRKFEHPRHGSLGFLPRKRAARHRGKVKAFPKDDPTKPPKLTAFLGYKAGMTHIVREVEKPGSKLHKKETCESVTIIETPPMVVVGVVGYVKTPRGLRTLNTVWTQHLSEEIKRRFYTDWCKSMQKAFRNYSKQYKSDEGKKNIQTQLEKLKKYATVIRVLAHTQIRKMKGLKQKKAHIMEIQVNGGTIAQKVDFAYSFFEKQVPIDAVFQKDEMIDVIGVTKGKGYEGVVTRWGVTRLPRKTHRGLRKVACIGAWHPARVSFTVARAGQNGYHHRTELNKKIYKLGKVGEETHGGQTEFDRTEKDITPMGGFPHYGIVKEDFLMIKGGCVGPKKRVITLRQSLLKQTSRLALEDIKLKFIDTSSKFGHGRFQTTSEKQKFYGRLKA