Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062005.t1 | XP_013453854.1 | 98.6 | 74 | 1 | 0 | 1 | 74 | 285 | 358 | 7.10E-32 | 146 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062005.t1 | D4N502 | 52.1 | 71 | 34 | 0 | 1 | 71 | 290 | 360 | 5.1e-15 | 81.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062005.t1 | A0A072UPY1 | 98.6 | 74 | 1 | 0 | 1 | 74 | 285 | 358 | 5.1e-32 | 146.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049222 | MS.gene062005 | -0.803813 | 2.91E-49 | -1.69E-46 |
| MS.gene04932 | MS.gene062005 | 0.813772 | 2.16E-51 | -1.69E-46 |
| MS.gene049549 | MS.gene062005 | 0.829309 | 5.53E-55 | -1.69E-46 |
| MS.gene050170 | MS.gene062005 | -0.826228 | 3.04E-54 | -1.69E-46 |
| MS.gene050323 | MS.gene062005 | -0.804662 | 1.94E-49 | -1.69E-46 |
| MS.gene050381 | MS.gene062005 | 0.812172 | 4.85E-51 | -1.69E-46 |
| MS.gene050504 | MS.gene062005 | -0.807071 | 6.05E-50 | -1.69E-46 |
| MS.gene050647 | MS.gene062005 | 0.842767 | 2.11E-58 | -1.69E-46 |
| MS.gene050868 | MS.gene062005 | -0.817617 | 3.01E-52 | -1.69E-46 |
| MS.gene050871 | MS.gene062005 | -0.821614 | 3.68E-53 | -1.69E-46 |
| MS.gene051127 | MS.gene062005 | 0.80624 | 9.06E-50 | -1.69E-46 |
| MS.gene051138 | MS.gene062005 | 0.818947 | 1.50E-52 | -1.69E-46 |
| MS.gene051651 | MS.gene062005 | 0.801948 | 7.07E-49 | -1.69E-46 |
| MS.gene051941 | MS.gene062005 | -0.805823 | 1.11E-49 | -1.69E-46 |
| MS.gene052164 | MS.gene062005 | -0.822731 | 2.03E-53 | -1.69E-46 |
| MS.gene052211 | MS.gene062005 | 0.823819 | 1.13E-53 | -1.69E-46 |
| MS.gene05240 | MS.gene062005 | 0.837337 | 5.52E-57 | -1.69E-46 |
| MS.gene05244 | MS.gene062005 | 0.80211 | 6.55E-49 | -1.69E-46 |
| MS.gene052606 | MS.gene062005 | 0.833936 | 4.00E-56 | -1.69E-46 |
| MS.gene053031 | MS.gene062005 | 0.83174 | 1.41E-55 | -1.69E-46 |
| MS.gene053033 | MS.gene062005 | 0.817723 | 2.84E-52 | -1.69E-46 |
| MS.gene053203 | MS.gene062005 | -0.809444 | 1.89E-50 | -1.69E-46 |
| MS.gene053403 | MS.gene062005 | 0.803514 | 3.36E-49 | -1.69E-46 |
| MS.gene053406 | MS.gene062005 | 0.845551 | 3.78E-59 | -1.69E-46 |
| MS.gene053420 | MS.gene062005 | 0.83086 | 2.31E-55 | -1.69E-46 |
| MS.gene053421 | MS.gene062005 | 0.800063 | 1.72E-48 | -1.69E-46 |
| MS.gene054146 | MS.gene062005 | 0.812657 | 3.80E-51 | -1.69E-46 |
| MS.gene054181 | MS.gene062005 | 0.85073 | 1.41E-60 | -1.69E-46 |
| MS.gene054706 | MS.gene062005 | 0.816234 | 6.14E-52 | -1.69E-46 |
| MS.gene05470 | MS.gene062005 | -0.817796 | 2.74E-52 | -1.69E-46 |
| MS.gene054742 | MS.gene062005 | 0.812864 | 3.42E-51 | -1.69E-46 |
| MS.gene055158 | MS.gene062005 | 0.832944 | 7.08E-56 | -1.69E-46 |
| MS.gene055159 | MS.gene062005 | 0.803967 | 2.71E-49 | -1.69E-46 |
| MS.gene055202 | MS.gene062005 | 0.808016 | 3.81E-50 | -1.69E-46 |
| MS.gene055240 | MS.gene062005 | 0.833555 | 4.98E-56 | -1.69E-46 |
| MS.gene055741 | MS.gene062005 | -0.800657 | 1.30E-48 | -1.69E-46 |
| MS.gene055744 | MS.gene062005 | -0.800549 | 1.37E-48 | -1.69E-46 |
| MS.gene05589 | MS.gene062005 | -0.841208 | 5.46E-58 | -1.69E-46 |
| MS.gene055959 | MS.gene062005 | 0.817538 | 3.13E-52 | -1.69E-46 |
| MS.gene056040 | MS.gene062005 | -0.810499 | 1.12E-50 | -1.69E-46 |
| MS.gene056166 | MS.gene062005 | -0.808205 | 3.47E-50 | -1.69E-46 |
| MS.gene056218 | MS.gene062005 | 0.853881 | 1.78E-61 | -1.69E-46 |
| MS.gene056298 | MS.gene062005 | 0.826438 | 2.71E-54 | -1.69E-46 |
| MS.gene056387 | MS.gene062005 | -0.823584 | 1.28E-53 | -1.69E-46 |
| MS.gene056504 | MS.gene062005 | -0.82953 | 4.89E-55 | -1.69E-46 |
| MS.gene056590 | MS.gene062005 | 0.820058 | 8.38E-53 | -1.69E-46 |
| MS.gene056591 | MS.gene062005 | 0.820695 | 5.99E-53 | -1.69E-46 |
| MS.gene056694 | MS.gene062005 | 0.803805 | 2.93E-49 | -1.69E-46 |
| MS.gene056748 | MS.gene062005 | 0.809931 | 1.48E-50 | -1.69E-46 |
| MS.gene056856 | MS.gene062005 | 0.804086 | 2.56E-49 | -1.69E-46 |
| MS.gene057019 | MS.gene062005 | -0.810448 | 1.15E-50 | -1.69E-46 |
| MS.gene057544 | MS.gene062005 | 0.814072 | 1.86E-51 | -1.69E-46 |
| MS.gene057768 | MS.gene062005 | 0.825173 | 5.41E-54 | -1.69E-46 |
| MS.gene058099 | MS.gene062005 | 0.816681 | 4.88E-52 | -1.69E-46 |
| MS.gene058109 | MS.gene062005 | 0.806185 | 9.30E-50 | -1.69E-46 |
| MS.gene058171 | MS.gene062005 | 0.84365 | 1.23E-58 | -1.69E-46 |
| MS.gene058173 | MS.gene062005 | 0.833005 | 6.84E-56 | -1.69E-46 |
| MS.gene058175 | MS.gene062005 | 0.843634 | 1.24E-58 | -1.69E-46 |
| MS.gene05837 | MS.gene062005 | -0.823804 | 1.14E-53 | -1.69E-46 |
| MS.gene058838 | MS.gene062005 | -0.816879 | 4.40E-52 | -1.69E-46 |
| MS.gene059037 | MS.gene062005 | 0.806266 | 8.94E-50 | -1.69E-46 |
| MS.gene059045 | MS.gene062005 | 0.814664 | 1.37E-51 | -1.69E-46 |
| MS.gene059328 | MS.gene062005 | 0.806735 | 7.12E-50 | -1.69E-46 |
| MS.gene059402 | MS.gene062005 | 0.822541 | 2.24E-53 | -1.69E-46 |
| MS.gene059667 | MS.gene062005 | -0.81697 | 4.20E-52 | -1.69E-46 |
| MS.gene059865 | MS.gene062005 | -0.809724 | 1.64E-50 | -1.69E-46 |
| MS.gene059953 | MS.gene062005 | 0.809168 | 2.16E-50 | -1.69E-46 |
| MS.gene059955 | MS.gene062005 | 0.806475 | 8.08E-50 | -1.69E-46 |
| MS.gene06000 | MS.gene062005 | -0.836101 | 1.14E-56 | -1.69E-46 |
| MS.gene060087 | MS.gene062005 | 0.800983 | 1.12E-48 | -1.69E-46 |
| MS.gene060372 | MS.gene062005 | -0.817903 | 2.59E-52 | -1.69E-46 |
| MS.gene060593 | MS.gene062005 | 0.821168 | 4.66E-53 | -1.69E-46 |
| MS.gene060595 | MS.gene062005 | 0.811027 | 8.60E-51 | -1.69E-46 |
| MS.gene060762 | MS.gene062005 | -0.80889 | 2.48E-50 | -1.69E-46 |
| MS.gene060778 | MS.gene062005 | -0.816236 | 6.14E-52 | -1.69E-46 |
| MS.gene06077 | MS.gene062005 | -0.818757 | 1.66E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 12 sgRNAs with CRISPR-Local
Find 16 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGAGTAATAAGGCTTGGTAC+TGG | 0.450966 | 5.1:-37470102 | None:intergenic |
| TATTTCTCACGCCAGCTTGA+AGG | 0.471903 | 5.1:+37470177 | MS.gene062005:CDS |
| CTAATGTCGTTGAACACTGC+AGG | 0.481272 | 5.1:-37470132 | None:intergenic |
| AGTGTGGAAGATTTCTTCAA+AGG | 0.508864 | 5.1:+37470153 | MS.gene062005:CDS |
| TAATGTCGTTGAACACTGCA+GGG | 0.582551 | 5.1:-37470131 | None:intergenic |
| CAATCTAGCACTGTGAAATG+TGG | 0.587201 | 5.1:-37470064 | None:intergenic |
| CGATTAACTCAGAGAAGGAG+AGG | 0.590103 | 5.1:+37470031 | MS.gene062005:CDS |
| AGCACTGTGAAATGTGGCAA+TGG | 0.625118 | 5.1:-37470058 | None:intergenic |
| AGCAACGATTAACTCAGAGA+AGG | 0.647001 | 5.1:+37470026 | MS.gene062005:CDS |
| AAATGTACATTGATGACATG+AGG | 0.655321 | 5.1:+37470202 | MS.gene062005:CDS |
| AATGTCGTTGAACACTGCAG+GGG | 0.691919 | 5.1:-37470130 | None:intergenic |
| AGTGTTCAACGACATTAGTG+TGG | 0.716232 | 5.1:+37470137 | MS.gene062005:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAATGTACATTGATGACATG+AGG | + | chr5.1:37470202-37470221 | MS.gene062005:CDS | 30.0% |
| AGTGTGGAAGATTTCTTCAA+AGG | + | chr5.1:37470153-37470172 | MS.gene062005:CDS | 35.0% | |
| ! | TGTACATTTTTCCTTCAAGC+TGG | - | chr5.1:37470191-37470210 | None:intergenic | 35.0% |
| !!! | GTTTTAGGAGTAATAAGGCT+TGG | - | chr5.1:37470111-37470130 | None:intergenic | 35.0% |
| AGCAACGATTAACTCAGAGA+AGG | + | chr5.1:37470026-37470045 | MS.gene062005:CDS | 40.0% | |
| AGTGTTCAACGACATTAGTG+TGG | + | chr5.1:37470137-37470156 | MS.gene062005:CDS | 40.0% | |
| CAATCTAGCACTGTGAAATG+TGG | - | chr5.1:37470067-37470086 | None:intergenic | 40.0% | |
| TAATGTCGTTGAACACTGCA+GGG | - | chr5.1:37470134-37470153 | None:intergenic | 40.0% | |
| !!! | CAGGGGTTTTAGGAGTAATA+AGG | - | chr5.1:37470116-37470135 | None:intergenic | 40.0% |
| AATGTCGTTGAACACTGCAG+GGG | - | chr5.1:37470133-37470152 | None:intergenic | 45.0% | |
| AGCACTGTGAAATGTGGCAA+TGG | - | chr5.1:37470061-37470080 | None:intergenic | 45.0% | |
| CGATTAACTCAGAGAAGGAG+AGG | + | chr5.1:37470031-37470050 | MS.gene062005:CDS | 45.0% | |
| CTAATGTCGTTGAACACTGC+AGG | - | chr5.1:37470135-37470154 | None:intergenic | 45.0% | |
| TATTTCTCACGCCAGCTTGA+AGG | + | chr5.1:37470177-37470196 | MS.gene062005:CDS | 45.0% | |
| ! | GGAGTAATAAGGCTTGGTAC+TGG | - | chr5.1:37470105-37470124 | None:intergenic | 45.0% |
| ! | TTGAACACTGCAGGGGTTTT+AGG | - | chr5.1:37470126-37470145 | None:intergenic | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 37470014 | 37470239 | 37470014 | ID=MS.gene062005 |
| chr5.1 | mRNA | 37470014 | 37470239 | 37470014 | ID=MS.gene062005.t1;Parent=MS.gene062005 |
| chr5.1 | exon | 37470014 | 37470239 | 37470014 | ID=MS.gene062005.t1.exon1;Parent=MS.gene062005.t1 |
| chr5.1 | CDS | 37470014 | 37470239 | 37470014 | ID=cds.MS.gene062005.t1;Parent=MS.gene062005.t1 |
| Gene Sequence |
| Protein sequence |
