AlfalfaGEDB | MS.gene063119

M. sativa cultivar XinJiangDaYe / MS.gene063119

KEGG: K19033
GO: GO:0005575 GO:0005840 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0110165
PFAM: PF17067.8

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene063119.t1	KEH36378.1	95.1	81	3	1	1	81	1	80	3.00E-32	147.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene063119.t1	P47909	57.7	71	27	1	11	81	4	71	4.5e-13	75.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene063119.t1	A0A072V4Y3	95.1	81	3	1	1	81	1	80	2.1e-32	147.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049255	MS.gene063119	0.844203	8.74E-59	-1.69E-46
MS.gene049341	MS.gene063119	0.815964	7.06E-52	-1.69E-46
MS.gene049382	MS.gene063119	0.828845	7.17E-55	-1.69E-46
MS.gene049756	MS.gene063119	0.834751	2.50E-56	-1.69E-46
MS.gene04984	MS.gene063119	0.817206	3.72E-52	-1.69E-46
MS.gene050277	MS.gene063119	0.869166	3.80E-66	-1.69E-46
MS.gene050386	MS.gene063119	0.800307	1.53E-48	-1.69E-46
MS.gene050437	MS.gene063119	0.803223	3.86E-49	-1.69E-46
MS.gene050586	MS.gene063119	0.831942	1.25E-55	-1.69E-46
MS.gene050623	MS.gene063119	-0.810056	1.39E-50	-1.69E-46
MS.gene050689	MS.gene063119	0.816145	6.43E-52	-1.69E-46
MS.gene050721	MS.gene063119	0.801924	7.16E-49	-1.69E-46
MS.gene050724	MS.gene063119	0.800901	1.16E-48	-1.69E-46
MS.gene050807	MS.gene063119	0.81774	2.82E-52	-1.69E-46
MS.gene050815	MS.gene063119	0.844892	5.70E-59	-1.69E-46
MS.gene050838	MS.gene063119	0.817312	3.52E-52	-1.69E-46
MS.gene051063	MS.gene063119	0.803043	4.21E-49	-1.69E-46
MS.gene051241	MS.gene063119	0.800415	1.46E-48	-1.69E-46
MS.gene051243	MS.gene063119	0.867013	1.87E-65	-1.69E-46
MS.gene051245	MS.gene063119	0.851863	6.73E-61	-1.69E-46
MS.gene051328	MS.gene063119	0.861939	7.21E-64	-1.69E-46
MS.gene051349	MS.gene063119	0.812111	5.00E-51	-1.69E-46
MS.gene05134	MS.gene063119	0.825076	5.71E-54	-1.69E-46
MS.gene051577	MS.gene063119	0.830468	2.89E-55	-1.69E-46
MS.gene051633	MS.gene063119	0.835388	1.73E-56	-1.69E-46
MS.gene051746	MS.gene063119	0.804891	1.74E-49	-1.69E-46
MS.gene051748	MS.gene063119	0.812451	4.21E-51	-1.69E-46
MS.gene051757	MS.gene063119	0.815395	9.45E-52	-1.69E-46
MS.gene051789	MS.gene063119	0.821685	3.54E-53	-1.69E-46
MS.gene051903	MS.gene063119	0.812352	4.43E-51	-1.69E-46
MS.gene052133	MS.gene063119	0.811161	8.04E-51	-1.69E-46
MS.gene052286	MS.gene063119	0.837674	4.52E-57	-1.69E-46
MS.gene052302	MS.gene063119	0.81598	7.00E-52	-1.69E-46
MS.gene052376	MS.gene063119	0.817939	2.54E-52	-1.69E-46
MS.gene052397	MS.gene063119	0.806863	6.69E-50	-1.69E-46
MS.gene052665	MS.gene063119	0.826035	3.38E-54	-1.69E-46
MS.gene052856	MS.gene063119	0.817695	2.89E-52	-1.69E-46
MS.gene052860	MS.gene063119	0.807069	6.05E-50	-1.69E-46
MS.gene052947	MS.gene063119	0.815136	1.08E-51	-1.69E-46
MS.gene053008	MS.gene063119	0.807312	5.38E-50	-1.69E-46
MS.gene053058	MS.gene063119	0.81161	6.42E-51	-1.69E-46
MS.gene053074	MS.gene063119	0.80801	3.82E-50	-1.69E-46
MS.gene053109	MS.gene063119	0.821659	3.59E-53	-1.69E-46
MS.gene053216	MS.gene063119	0.804247	2.37E-49	-1.69E-46
MS.gene053630	MS.gene063119	0.804077	2.57E-49	-1.69E-46
MS.gene054823	MS.gene063119	0.809926	1.49E-50	-1.69E-46
MS.gene055133	MS.gene063119	0.831852	1.32E-55	-1.69E-46
MS.gene055174	MS.gene063119	0.801534	8.61E-49	-1.69E-46
MS.gene055207	MS.gene063119	0.819234	1.29E-52	-1.69E-46
MS.gene055441	MS.gene063119	0.82231	2.54E-53	-1.69E-46
MS.gene055512	MS.gene063119	0.801226	9.95E-49	-1.69E-46
MS.gene056069	MS.gene063119	-0.818106	2.33E-52	-1.69E-46
MS.gene056432	MS.gene063119	0.823916	1.07E-53	-1.69E-46
MS.gene056580	MS.gene063119	0.83783	4.12E-57	-1.69E-46
MS.gene056631	MS.gene063119	0.842939	1.90E-58	-1.69E-46
MS.gene056760	MS.gene063119	0.847891	8.68E-60	-1.69E-46
MS.gene056787	MS.gene063119	0.801833	7.47E-49	-1.69E-46
MS.gene057057	MS.gene063119	0.854034	1.61E-61	-1.69E-46
MS.gene057140	MS.gene063119	0.865863	4.34E-65	-1.69E-46
MS.gene057234	MS.gene063119	0.851492	8.57E-61	-1.69E-46
MS.gene057754	MS.gene063119	0.818936	1.51E-52	-1.69E-46
MS.gene05795	MS.gene063119	0.802017	6.85E-49	-1.69E-46
MS.gene058055	MS.gene063119	0.837548	4.87E-57	-1.69E-46
MS.gene058084	MS.gene063119	0.822901	1.85E-53	-1.69E-46
MS.gene058182	MS.gene063119	0.823712	1.20E-53	-1.69E-46
MS.gene058238	MS.gene063119	0.835342	1.77E-56	-1.69E-46
MS.gene058399	MS.gene063119	0.807515	4.87E-50	-1.69E-46
MS.gene058646	MS.gene063119	0.826656	2.41E-54	-1.69E-46
MS.gene058654	MS.gene063119	0.810739	9.92E-51	-1.69E-46
MS.gene058906	MS.gene063119	0.878899	1.93E-69	-1.69E-46
MS.gene05978	MS.gene063119	0.802617	5.15E-49	-1.69E-46
MS.gene059898	MS.gene063119	0.817154	3.82E-52	-1.69E-46
MS.gene06001	MS.gene063119	0.845452	4.03E-59	-1.69E-46
MS.gene060084	MS.gene063119	0.803457	3.45E-49	-1.69E-46
MS.gene060165	MS.gene063119	0.803996	2.67E-49	-1.69E-46
MS.gene060297	MS.gene063119	0.804228	2.39E-49	-1.69E-46
MS.gene060429	MS.gene063119	0.808627	2.82E-50	-1.69E-46
MS.gene060465	MS.gene063119	0.806729	7.14E-50	-1.69E-46
MS.gene060803	MS.gene063119	0.806408	8.35E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene063119.t1	MTR_3g117570	93.902	82	4	1	1	82	1	81	2.05e-49	152
MS.gene063119.t1	MTR_3g117570	95.062	81	3	1	1	81	1	80	6.40e-49	151

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 26 sgRNAs with CRISPR-Local

Find 67 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACTCACAAATACAGATTGTT+TGG	0.302653	3.4:-99885880	MS.gene063119:intron
ATAACTACTTCTCTTGGTTA+TGG	0.376607	3.4:-99885820	MS.gene063119:CDS
TCGATCATTTGTTCTCTCTT+CGG	0.404252	3.4:+99886555	None:intergenic
AAAAGATTCAAGGGCTCGTA+TGG	0.464506	3.4:-99886588	MS.gene063119:CDS
AAAGAAAGGGAAAAGATTCA+AGG	0.481880	3.4:-99886598	MS.gene063119:CDS
ACGCCTTGTGCTTCTTCTGC+CGG	0.494602	3.4:-99886666	MS.gene063119:CDS
GAGTGCATAACTACTTCTCT+TGG	0.501421	3.4:-99885826	MS.gene063119:CDS
GCTGCACGGTGGTTGATAAG+AGG	0.503001	3.4:-99886702	MS.gene063119:CDS
GATCGAACGAATCAAAGATA+AGG	0.533385	3.4:-99886538	MS.gene063119:CDS
GCTCCGATAGTGTGCGGACG+CGG	0.535798	3.4:-99886633	MS.gene063119:CDS
TCACCGCGTCCGCACACTAT+CGG	0.538358	3.4:+99886630	None:intergenic
TATCAACCACCGTGCAGCTA+CGG	0.541002	3.4:+99886707	None:intergenic
AAGATAAGGTTGAAGTTCCC+AGG	0.546325	3.4:-99886524	MS.gene063119:intron
AGGAATTTCGATAAAAGAAG+TGG	0.550885	3.4:-99885797	MS.gene063119:CDS
TATCGGAGCAGCTGTTACGC+CGG	0.576176	3.4:+99886647	None:intergenic
AAGAAAGGGAAAAGATTCAA+GGG	0.576778	3.4:-99886597	MS.gene063119:CDS
ACTACTTCTCTTGGTTATGG+AGG	0.586505	3.4:-99885817	MS.gene063119:CDS
AGCGGTGCACCGTAGCTGCA+CGG	0.586905	3.4:-99886716	MS.gene063119:CDS
GTTGATAAGAGGAGCGTCGT+CGG	0.589779	3.4:-99886691	MS.gene063119:CDS
AAAGATTCAAGGGCTCGTAT+GGG	0.635720	3.4:-99886587	MS.gene063119:CDS
GTGGCGCTAGTGTGATGCAG+CGG	0.656151	3.4:-99886734	MS.gene063119:CDS
AAATTGTGTATGGCGAGTGG+TGG	0.695087	3.4:-99886753	None:intergenic
ACAGCTGCTCCGATAGTGTG+CGG	0.696198	3.4:-99886639	MS.gene063119:CDS
AGAAAATTGTGTATGGCGAG+TGG	0.703620	3.4:-99886756	None:intergenic
ACGCCGGCAGAAGAAGCACA+AGG	0.711011	3.4:+99886663	None:intergenic
GGTGCACCGTAGCTGCACGG+TGG	0.772757	3.4:-99886713	MS.gene063119:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AATTGTTCATATAGATATAT+AGG	-	chr3.4:99886545-99886564	MS.gene063119:CDS	15.0%
!!!	ATTTAAAATCGAAATCCATA+AGG	-	chr3.4:99886358-99886377	MS.gene063119:intron	20.0%
!!!	TTTTCTGTTGAAATAGAAAA+TGG	+	chr3.4:99886115-99886134	None:intergenic	20.0%
!	GTGATAAAAAGACAAAGAAA+GGG	-	chr3.4:99885907-99885926	MS.gene063119:intron	25.0%
!	TAGTTTGAGAATACTACTAA+CGG	-	chr3.4:99886445-99886464	MS.gene063119:intron	25.0%
!	TTTGATGCATTATGTTATGT+TGG	-	chr3.4:99886141-99886160	MS.gene063119:intron	25.0%
!!	AAGTATTTGAAGTGAATTAG+AGG	+	chr3.4:99886328-99886347	None:intergenic	25.0%
!!	AGTATTTGAAGTGAATTAGA+GGG	+	chr3.4:99886327-99886346	None:intergenic	25.0%
!!	TCTTGCAAATTTTCCAAAAT+GGG	-	chr3.4:99886491-99886510	MS.gene063119:intron	25.0%
!!!	TCATTTCATTCCCATTTTTA+GGG	-	chr3.4:99886055-99886074	MS.gene063119:intron	25.0%
!!!	TTCATTTCATTCCCATTTTT+AGG	-	chr3.4:99886054-99886073	MS.gene063119:intron	25.0%
	AAACAATCTGTATTTGTGAG+TGG	+	chr3.4:99886639-99886658	None:intergenic	30.0%
	AAAGAAAGGGAAAAGATTCA+AGG	-	chr3.4:99885920-99885939	MS.gene063119:intron	30.0%
	AAATGCTAGTAATGTTTGTG+GGG	+	chr3.4:99886417-99886436	None:intergenic	30.0%
	AAGAAAGGGAAAAGATTCAA+GGG	-	chr3.4:99885921-99885940	MS.gene063119:intron	30.0%
	ACTCACAAATACAGATTGTT+TGG	-	chr3.4:99886638-99886657	MS.gene063119:CDS	30.0%
	AGAAATGCTAGTAATGTTTG+TGG	+	chr3.4:99886419-99886438	None:intergenic	30.0%
	AGGAATTTCGATAAAAGAAG+TGG	-	chr3.4:99886721-99886740	MS.gene063119:CDS	30.0%
	ATAACTACTTCTCTTGGTTA+TGG	-	chr3.4:99886698-99886717	MS.gene063119:CDS	30.0%
	GAAATGCTAGTAATGTTTGT+GGG	+	chr3.4:99886418-99886437	None:intergenic	30.0%
	GGTGATAAAAAGACAAAGAA+AGG	-	chr3.4:99885906-99885925	MS.gene063119:intron	30.0%
	TGAAATCAAATGAGCTTGAA+AGG	+	chr3.4:99886038-99886057	None:intergenic	30.0%
!	AAAATGGCAATGAAAGTACA+TGG	+	chr3.4:99886099-99886118	None:intergenic	30.0%
!	GTCTTGCAAATTTTCCAAAA+TGG	-	chr3.4:99886490-99886509	MS.gene063119:intron	30.0%
!	TAAATTTGTGACTTGCAAGT+TGG	+	chr3.4:99886390-99886409	None:intergenic	30.0%
!!!	GACTTTATTTGTTTTGTGAG+TGG	+	chr3.4:99886473-99886492	None:intergenic	30.0%
	AATTCCACAGTGTTGTGTTT+GGG	-	chr3.4:99886175-99886194	MS.gene063119:intron	35.0%
	AGACGAGAAAACCCTAAAAA+TGG	+	chr3.4:99886069-99886088	None:intergenic	35.0%
	ATATAGGACACACAGATTCA+TGG	-	chr3.4:99886561-99886580	MS.gene063119:CDS	35.0%
	GACGAGAAAACCCTAAAAAT+GGG	+	chr3.4:99886068-99886087	None:intergenic	35.0%
	GATCGAACGAATCAAAGATA+AGG	-	chr3.4:99885980-99885999	MS.gene063119:intron	35.0%
	TAATTCCACAGTGTTGTGTT+TGG	-	chr3.4:99886174-99886193	MS.gene063119:intron	35.0%
	TCCTTACTCAAAGTTAGTGA+GGG	-	chr3.4:99886590-99886609	MS.gene063119:CDS	35.0%
	TCGATCATTTGTTCTCTCTT+CGG	+	chr3.4:99885966-99885985	None:intergenic	35.0%
!	GTAAAATAGCATGCCCATTT+TGG	+	chr3.4:99886507-99886526	None:intergenic	35.0%
!!!	AAATGGGCATGCTATTTTAC+TGG	-	chr3.4:99886507-99886526	MS.gene063119:intron	35.0%
!!!	AATGGGCATGCTATTTTACT+GGG	-	chr3.4:99886508-99886527	MS.gene063119:intron	35.0%
	AAAACCCAAACACAACACTG+TGG	+	chr3.4:99886182-99886201	None:intergenic	40.0%
	AAAAGATTCAAGGGCTCGTA+TGG	-	chr3.4:99885930-99885949	MS.gene063119:intron	40.0%
	AAAGATTCAAGGGCTCGTAT+GGG	-	chr3.4:99885931-99885950	MS.gene063119:intron	40.0%
	AAGATAAGGTTGAAGTTCCC+AGG	-	chr3.4:99885994-99886013	MS.gene063119:intron	40.0%
	ACTACTTCTCTTGGTTATGG+AGG	-	chr3.4:99886701-99886720	MS.gene063119:CDS	40.0%
	CAAATGAGCTTGAAAGGAAG+AGG	+	chr3.4:99886032-99886051	None:intergenic	40.0%
	CCTTACTCAAAGTTAGTGAG+GGG	-	chr3.4:99886591-99886610	MS.gene063119:CDS	40.0%
	CTTACTCAAAGTTAGTGAGG+GGG	-	chr3.4:99886592-99886611	MS.gene063119:CDS	40.0%
	GAGTGCATAACTACTTCTCT+TGG	-	chr3.4:99886692-99886711	MS.gene063119:CDS	40.0%
	GTCCTTACTCAAAGTTAGTG+AGG	-	chr3.4:99886589-99886608	MS.gene063119:CDS	40.0%
!!	GCAAGTTGGTTTCAACCTTA+TGG	+	chr3.4:99886376-99886395	None:intergenic	40.0%
	CCCCTCACTAACTTTGAGTA+AGG	+	chr3.4:99886594-99886613	None:intergenic	45.0%
	AAGTTCCCAGGTCTACTCCT+TGG	-	chr3.4:99886006-99886025	MS.gene063119:intron	50.0%
	AGCTTGAAAGGAAGAGGCCA+AGG	+	chr3.4:99886026-99886045	None:intergenic	50.0%
	GGGGTCATTCTTGGTTGATC+TGG	-	chr3.4:99886611-99886630	MS.gene063119:CDS	50.0%
	GTTAGTGAGGGGGTCATTCT+TGG	-	chr3.4:99886602-99886621	MS.gene063119:CDS	50.0%
	TATCAACCACCGTGCAGCTA+CGG	+	chr3.4:99885814-99885833	None:intergenic	50.0%
!!	GTTGATAAGAGGAGCGTCGT+CGG	-	chr3.4:99885827-99885846	MS.gene063119:CDS	50.0%
	AAGAGGCCAAGGAGTAGACC+TGG	+	chr3.4:99886015-99886034	None:intergenic	55.0%
	ACAGCTGCTCCGATAGTGTG+CGG	-	chr3.4:99885879-99885898	MS.gene063119:intron	55.0%
	ACGCCTTGTGCTTCTTCTGC+CGG	-	chr3.4:99885852-99885871	MS.gene063119:CDS	55.0%
	AGAGGCCAAGGAGTAGACCT+GGG	+	chr3.4:99886014-99886033	None:intergenic	55.0%
	TATCGGAGCAGCTGTTACGC+CGG	+	chr3.4:99885874-99885893	None:intergenic	55.0%
!!	GCTGCACGGTGGTTGATAAG+AGG	-	chr3.4:99885816-99885835	MS.gene063119:CDS	55.0%
	GTGGCGCTAGTGTGATGCAG+CGG	-	chr3.4:99885784-99885803	MS.gene063119:CDS	60.0%
	TCACCGCGTCCGCACACTAT+CGG	+	chr3.4:99885891-99885910	None:intergenic	60.0%
!	ACGCCGGCAGAAGAAGCACA+AGG	+	chr3.4:99885858-99885877	None:intergenic	60.0%
	AGCGGTGCACCGTAGCTGCA+CGG	-	chr3.4:99885802-99885821	MS.gene063119:CDS	65.0%
	GCTCCGATAGTGTGCGGACG+CGG	-	chr3.4:99885885-99885904	MS.gene063119:intron	65.0%
	GGTGCACCGTAGCTGCACGG+TGG	-	chr3.4:99885805-99885824	MS.gene063119:CDS	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.4	gene	99885774	99886766	99885774	ID=MS.gene063119
chr3.4	mRNA	99885774	99886766	99885774	ID=MS.gene063119.t1;Parent=MS.gene063119
chr3.4	exon	99886525	99886766	99886525	ID=MS.gene063119.t1.exon1;Parent=MS.gene063119.t1
chr3.4	CDS	99886525	99886766	99886525	ID=cds.MS.gene063119.t1;Parent=MS.gene063119.t1
chr3.4	exon	99885774	99885888	99885774	ID=MS.gene063119.t1.exon2;Parent=MS.gene063119.t1
chr3.4	CDS	99885774	99885888	99885774	ID=cds.MS.gene063119.t1;Parent=MS.gene063119.t1

Gene Sequence

>MS.gene063119

ATGGCGAGTGGTGGCGCTAGTGTGATGCAGCGGTGCACCGTAGCTGCACGGTGGTTGATAAGAGGAGCGTCGTCGGAGACGCCTTGTGCTTCTTCTGCCGGCGTAACAGCTGCTCCGATAGTGTGCGGACGCGGTGATAAAAAGACAAAGAAAGGGAAAAGATTCAAGGGCTCGTATGGGAAATCTCGTCCGAAGAGAGAACAAATGATCGAACGAATCAAAGATAAGGTTGAAGTTCCCAGGTCTACTCCTTGGCCTCTTCCTTTCAAGCTCATTTGATTTCATTTCATTCCCATTTTTAGGGTTTTCTCGTCTGAACAATCCATGTACTTTCATTGCCATTTTCTATTTCAACAGAAAATTTTATTTTGATGCATTATGTTATGTTGGTTGCTCATTGTAATTCCACAGTGTTGTGTTTGGGTTTTATCAATCATTACAAACAAATTAATAAATTTGCTGTGCAATATTGTTGTTTATGCTAATAGATTAATCATTTCACTCTCACAGACACTTGTATAGAAAAATATCGTAAATATGAAAAGTAAAGCCCTCTAATTCACTTCAAATACTTAATCAGATTGATTTAAAATCGAAATCCATAAGGTTGAAACCAACTTGCAAGTCACAAATTTAAGAGCCCCACAAACATTACTAGCATTTCTAGAAAATAGTTTGAGAATACTACTAACGGAACCACTCACAAAACAAATAAAGTCTTGCAAATTTTCCAAAATGGGCATGCTATTTTACTGGGATTCGTTTTTTAGAAATTGTTCATATAGATATATAGGACACACAGATTCATGGACACAGTCCTTACTCAAAGTTAGTGAGGGGGTCATTCTTGGTTGATCTGGTTCCACTCACAAATACAGATTGTTTGGAATGTGCACTGCAGCATTTGCAGAGAAATTAGAGTGCATAACTACTTCTCTTGGTTATGGAGGAATTTCGATAAAAGAAGTGGACAGTTCTTGTATAAAATGCTAA

Protein sequence

>MS.gene063119.t1

MASGGASVMQRCTVAARWLIRGASSETPCASSAGVTAAPIVCGRGDKKTKKGKRFKGSYGKSRPKREQMIERIKDKVEVPRLFGMCTAAFAEKLECITTSLGYGGISIKEVDSSCIKC