AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene069487


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene069487.t1 MTR_6g078280 96.226 212 8 0 1 212 1 212 8.50e-155 427
MS.gene069487.t1 MTR_6g078260 69.154 201 42 5 15 212 16 199 5.82e-95 275
MS.gene069487.t1 MTR_6g078140 59.701 201 73 5 15 211 16 212 2.04e-74 224
MS.gene069487.t1 MTR_6g078120 59.701 201 73 5 15 211 16 212 1.62e-73 221
MS.gene069487.t1 MTR_6g078290 52.558 215 92 6 1 210 1 210 5.10e-68 207
MS.gene069487.t1 MTR_6g078250 53.023 215 87 5 1 212 1 204 1.56e-65 201
MS.gene069487.t1 MTR_7g037410 44.444 189 90 5 26 211 25 201 5.08e-50 161
MS.gene069487.t1 MTR_6g078100 38.462 221 109 10 1 211 1 204 1.16e-35 125
MS.gene069487.t1 MTR_6g078070 37.156 218 114 9 1 211 1 202 2.55e-32 116
MS.gene069487.t1 MTR_0211s0080 29.577 213 119 10 9 211 6 197 1.47e-13 67.0
MS.gene069487.t1 MTR_6g009563 30.097 206 121 7 3 203 6 193 5.20e-13 65.5
MS.gene069487.t1 MTR_3g106620 31.111 225 111 13 6 212 1 199 5.30e-13 65.9
MS.gene069487.t1 MTR_8g461050 30.189 212 123 10 9 211 6 201 6.22e-13 65.5
MS.gene069487.t1 MTR_0100s0150 29.108 213 123 10 9 211 6 200 7.20e-13 65.1
MS.gene069487.t1 MTR_0084s0030 30.374 214 120 12 9 211 6 201 8.96e-13 64.7
MS.gene069487.t1 MTR_6g009650 31.053 190 107 7 23 203 18 192 7.52e-12 62.4
MS.gene069487.t1 MTR_6g066190 30.168 179 105 6 30 203 29 192 1.73e-11 61.6
MS.gene069487.t1 MTR_3g106585 29.302 215 119 12 5 212 9 197 1.98e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene069487.t1 AT1G17860 37.963 216 107 9 1 211 1 194 3.08e-39 134
MS.gene069487.t1 AT1G73325 32.889 225 114 11 1 211 1 202 4.56e-22 90.1
MS.gene069487.t1 AT1G73260 31.280 211 122 11 8 212 12 205 6.93e-20 84.3
MS.gene069487.t1 AT1G73330 30.317 221 116 13 1 212 1 192 6.06e-15 70.9

Find 51 sgRNAs with CRISPR-Local

Find 51 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCTGAAACTTAACTTTAA+AGG 0.154767 6.4:+53324097 None:intergenic
CATCTTCATACTTCTCAATT+TGG 0.163418 6.4:+53324234 None:intergenic
ACAAAGCCACATATTGTAAA+TGG 0.283069 6.4:+53324557 None:intergenic
AATGTTTCTTCTTTAGAAAA+AGG 0.285974 6.4:+53324314 None:intergenic
TTCTGAAACTTAACTTTAAA+GGG 0.298093 6.4:+53324098 None:intergenic
AGCCGGAATGATGTAGTAAT+TGG 0.358723 6.4:+53324583 None:intergenic
AACCATGTATGCAAAGATAT+TGG 0.371437 6.4:-53324170 MS.gene069487:CDS
AACGCCTTTCTTGTTGTTAA+CGG 0.386453 6.4:+53324427 None:intergenic
GTGACTACTAATGGTGTATT+GGG 0.404413 6.4:-53324290 MS.gene069487:CDS
ACGCCTTTCTTGTTGTTAAC+GGG 0.404872 6.4:+53324428 None:intergenic
TTCCAATATCTTTGCATACA+TGG 0.418385 6.4:+53324168 None:intergenic
GAAACATTTGTGACTACTAA+TGG 0.419288 6.4:-53324299 MS.gene069487:CDS
TGCATACATGGTTGCAAGTA+GGG 0.429484 6.4:+53324180 None:intergenic
GCTAAGCCATTTACAATATG+TGG 0.430563 6.4:-53324563 MS.gene069487:CDS
TGTGACTACTAATGGTGTAT+TGG 0.442930 6.4:-53324291 MS.gene069487:CDS
TTGTCTTCGCAACCACTACT+TGG 0.447050 6.4:-53324668 MS.gene069487:CDS
TACCAATTACTACATCATTC+CGG 0.457107 6.4:-53324585 MS.gene069487:CDS
TAAGACTAGGGAAATGCGTT+TGG 0.471251 6.4:-53324135 MS.gene069487:CDS
TTTGTTAGTTGTTTCAATAG+TGG 0.473075 6.4:-53324539 MS.gene069487:CDS
GTTCAAATACAATCCACAAT+TGG 0.476445 6.4:-53324259 MS.gene069487:CDS
CATATTGTAAATGGCTTAGC+CGG 0.479615 6.4:+53324566 None:intergenic
TCTCAATTTGGAACCAATTG+TGG 0.486271 6.4:+53324246 None:intergenic
CGATGTGTCAATGACTTGAT+CGG 0.502159 6.4:+53324628 None:intergenic
GAACTTTATGTTGAGATCAG+TGG 0.505826 6.4:+53324394 None:intergenic
TTGCAAGTAGGGCACACATT+AGG 0.510934 6.4:+53324191 None:intergenic
TTGCATACATGGTTGCAAGT+AGG 0.520717 6.4:+53324179 None:intergenic
GTAGTTGTGAAACATAATCA+AGG 0.526996 6.4:-53324470 MS.gene069487:CDS
AGGATCAATCTTCCACACTA+AGG 0.534325 6.4:+53324334 None:intergenic
TTGTGGATTGTATTTGAACC+CGG 0.536118 6.4:+53324263 None:intergenic
GTATGAAGATGCTTATAAGT+TGG 0.537055 6.4:-53324222 MS.gene069487:CDS
GAATTTACGTGTATAAGACT+AGG 0.540719 6.4:-53324148 MS.gene069487:CDS
ATAATCAAGGTTTACCGTTG+AGG 0.542322 6.4:-53324457 MS.gene069487:CDS
TTCCACACTAAGGAATGGTT+AGG 0.542536 6.4:+53324344 None:intergenic
GTTAGTTGTTTCAATAGTGG+AGG 0.542635 6.4:-53324536 MS.gene069487:CDS
GATGTGTCAATGACTTGATC+GGG 0.551157 6.4:+53324629 None:intergenic
TAATGGTGTATTGGGAAACC+CGG 0.551726 6.4:-53324282 MS.gene069487:CDS
CTTTAAAGTTAAGTTTCAGA+AGG 0.553919 6.4:-53324096 MS.gene069487:CDS
GTCCTAACCATTCCTTAGTG+TGG 0.567409 6.4:-53324346 MS.gene069487:CDS
ACACCCGTTAACAACAAGAA+AGG 0.576147 6.4:-53324431 MS.gene069487:CDS
AATTTACGTGTATAAGACTA+GGG 0.580702 6.4:-53324147 MS.gene069487:CDS
GATCAAGTCATTGACACATC+GGG 0.588958 6.4:-53324626 MS.gene069487:CDS
TTCACAACTACTACATCAAG+AGG 0.592646 6.4:+53324482 None:intergenic
GGCATTGCTCTTGAAACCGT+CGG 0.595900 6.4:-53324515 MS.gene069487:CDS
CAATCTTCCACACTAAGGAA+TGG 0.596624 6.4:+53324339 None:intergenic
TGTGGATTGTATTTGAACCC+GGG 0.605984 6.4:+53324264 None:intergenic
GCTTCAGTTGATCCAAGTAG+TGG 0.616203 6.4:+53324656 None:intergenic
CGATCAAGTCATTGACACAT+CGG 0.616565 6.4:-53324627 MS.gene069487:CDS
TTAACGGGTGTGAACCTCAA+CGG 0.621698 6.4:+53324443 None:intergenic
AAGAGGGCAAGATTCTCCGA+CGG 0.655925 6.4:+53324499 None:intergenic
AATGGTGTATTGGGAAACCC+GGG 0.666072 6.4:-53324281 MS.gene069487:CDS
TCACAACTACTACATCAAGA+GGG 0.703659 6.4:+53324483 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATGTTTCTTCTTTAGAAAA+AGG + chr6.4:53324487-53324506 None:intergenic 20.0%
!!! TTCTGAAACTTAACTTTAAA+GGG + chr6.4:53324703-53324722 None:intergenic 20.0%
! AATTTACGTGTATAAGACTA+GGG - chr6.4:53324651-53324670 MS.gene069487:CDS 25.0%
!! TTTGTTAGTTGTTTCAATAG+TGG - chr6.4:53324259-53324278 MS.gene069487:CDS 25.0%
!!! CTTCTGAAACTTAACTTTAA+AGG + chr6.4:53324704-53324723 None:intergenic 25.0%
!!! CTTTAAAGTTAAGTTTCAGA+AGG - chr6.4:53324702-53324721 MS.gene069487:CDS 25.0%
AACCATGTATGCAAAGATAT+TGG - chr6.4:53324628-53324647 MS.gene069487:CDS 30.0%
ACAAAGCCACATATTGTAAA+TGG + chr6.4:53324244-53324263 None:intergenic 30.0%
CATCTTCATACTTCTCAATT+TGG + chr6.4:53324567-53324586 None:intergenic 30.0%
GAAACATTTGTGACTACTAA+TGG - chr6.4:53324499-53324518 MS.gene069487:CDS 30.0%
GAATTTACGTGTATAAGACT+AGG - chr6.4:53324650-53324669 MS.gene069487:CDS 30.0%
GTAGTTGTGAAACATAATCA+AGG - chr6.4:53324328-53324347 MS.gene069487:CDS 30.0%
GTATGAAGATGCTTATAAGT+TGG - chr6.4:53324576-53324595 MS.gene069487:CDS 30.0%
GTTCAAATACAATCCACAAT+TGG - chr6.4:53324539-53324558 MS.gene069487:CDS 30.0%
TACCAATTACTACATCATTC+CGG - chr6.4:53324213-53324232 MS.gene069487:CDS 30.0%
TTCCAATATCTTTGCATACA+TGG + chr6.4:53324633-53324652 None:intergenic 30.0%
ATAATCAAGGTTTACCGTTG+AGG - chr6.4:53324341-53324360 MS.gene069487:CDS 35.0%
CATATTGTAAATGGCTTAGC+CGG + chr6.4:53324235-53324254 None:intergenic 35.0%
GAACTTTATGTTGAGATCAG+TGG + chr6.4:53324407-53324426 None:intergenic 35.0%
GCTAAGCCATTTACAATATG+TGG - chr6.4:53324235-53324254 MS.gene069487:CDS 35.0%
GTGACTACTAATGGTGTATT+GGG - chr6.4:53324508-53324527 MS.gene069487:CDS 35.0%
TCACAACTACTACATCAAGA+GGG + chr6.4:53324318-53324337 None:intergenic 35.0%
TGTGACTACTAATGGTGTAT+TGG - chr6.4:53324507-53324526 MS.gene069487:CDS 35.0%
TTCACAACTACTACATCAAG+AGG + chr6.4:53324319-53324338 None:intergenic 35.0%
! GTTAGTTGTTTCAATAGTGG+AGG - chr6.4:53324262-53324281 MS.gene069487:CDS 35.0%
! TCTCAATTTGGAACCAATTG+TGG + chr6.4:53324555-53324574 None:intergenic 35.0%
! TTGTGGATTGTATTTGAACC+CGG + chr6.4:53324538-53324557 None:intergenic 35.0%
!! AACGCCTTTCTTGTTGTTAA+CGG + chr6.4:53324374-53324393 None:intergenic 35.0%
ACACCCGTTAACAACAAGAA+AGG - chr6.4:53324367-53324386 MS.gene069487:CDS 40.0%
AGCCGGAATGATGTAGTAAT+TGG + chr6.4:53324218-53324237 None:intergenic 40.0%
AGGATCAATCTTCCACACTA+AGG + chr6.4:53324467-53324486 None:intergenic 40.0%
CAATCTTCCACACTAAGGAA+TGG + chr6.4:53324462-53324481 None:intergenic 40.0%
CGATCAAGTCATTGACACAT+CGG - chr6.4:53324171-53324190 MS.gene069487:CDS 40.0%
CGATGTGTCAATGACTTGAT+CGG + chr6.4:53324173-53324192 None:intergenic 40.0%
GATCAAGTCATTGACACATC+GGG - chr6.4:53324172-53324191 MS.gene069487:CDS 40.0%
GATGTGTCAATGACTTGATC+GGG + chr6.4:53324172-53324191 None:intergenic 40.0%
TAAGACTAGGGAAATGCGTT+TGG - chr6.4:53324663-53324682 MS.gene069487:CDS 40.0%
TAATGGTGTATTGGGAAACC+CGG - chr6.4:53324516-53324535 MS.gene069487:CDS 40.0%
TGCATACATGGTTGCAAGTA+GGG + chr6.4:53324621-53324640 None:intergenic 40.0%
TTCCACACTAAGGAATGGTT+AGG + chr6.4:53324457-53324476 None:intergenic 40.0%
TTGCATACATGGTTGCAAGT+AGG + chr6.4:53324622-53324641 None:intergenic 40.0%
! TGTGGATTGTATTTGAACCC+GGG + chr6.4:53324537-53324556 None:intergenic 40.0%
!! ACGCCTTTCTTGTTGTTAAC+GGG + chr6.4:53324373-53324392 None:intergenic 40.0%
AATGGTGTATTGGGAAACCC+GGG - chr6.4:53324517-53324536 MS.gene069487:CDS 45.0%
GCTTCAGTTGATCCAAGTAG+TGG + chr6.4:53324145-53324164 None:intergenic 45.0%
GTCCTAACCATTCCTTAGTG+TGG - chr6.4:53324452-53324471 MS.gene069487:CDS 45.0%
TTGCAAGTAGGGCACACATT+AGG + chr6.4:53324610-53324629 None:intergenic 45.0%
TTGTCTTCGCAACCACTACT+TGG - chr6.4:53324130-53324149 MS.gene069487:CDS 45.0%
! TTAACGGGTGTGAACCTCAA+CGG + chr6.4:53324358-53324377 None:intergenic 45.0%
AAGAGGGCAAGATTCTCCGA+CGG + chr6.4:53324302-53324321 None:intergenic 50.0%
GGCATTGCTCTTGAAACCGT+CGG - chr6.4:53324283-53324302 MS.gene069487:CDS 50.0%


Chromosome Type Strat End Strand Name
chr6.4 gene 53324091 53324729 53324091 ID=MS.gene069487
chr6.4 mRNA 53324091 53324729 53324091 ID=MS.gene069487.t1;Parent=MS.gene069487
chr6.4 exon 53324091 53324729 53324091 ID=MS.gene069487.t1.exon1;Parent=MS.gene069487.t1
chr6.4 CDS 53324091 53324729 53324091 ID=cds.MS.gene069487.t1;Parent=MS.gene069487.t1
Gene Sequence

>MS.gene069487

ATGAAGAGTACAATGCTAGCATTTCTTCTTCTCTTTGCATTGTCTTCGCAACCACTACTTGGATCAACTGAAGCTTCACCCGATCAAGTCATTGACACATCGGGCAAGAAGCTTCGAGCTGATACCAATTACTACATCATTCCGGCTAAGCCATTTACAATATGTGGCTTTGTTAGTTGTTTCAATAGTGGAGGCATTGCTCTTGAAACCGTCGGAGAATCTTGCCCTCTTGATGTAGTAGTTGTGAAACATAATCAAGGTTTACCGTTGAGGTTCACACCCGTTAACAACAAGAAAGGCGTTATTCGTGTCTCCACTGATCTCAACATAAAGTTCTCTAATGATGCTTATGATTCTAGATGTCCTAACCATTCCTTAGTGTGGAAGATTGATCCTTTTTCTAAAGAAGAAACATTTGTGACTACTAATGGTGTATTGGGAAACCCGGGTTCAAATACAATCCACAATTGGTTCCAAATTGAGAAGTATGAAGATGCTTATAAGTTGGTCTATTGTCCTAATGTGTGCCCTACTTGCAACCATGTATGCAAAGATATTGGAATTTACGTGTATAAGACTAGGGAAATGCGTTTGGCTCTCACTAATGTTCCCTTTAAAGTTAAGTTTCAGAAGGTTTGA

Protein sequence

>MS.gene069487.t1

MKSTMLAFLLLFALSSQPLLGSTEASPDQVIDTSGKKLRADTNYYIIPAKPFTICGFVSCFNSGGIALETVGESCPLDVVVVKHNQGLPLRFTPVNNKKGVIRVSTDLNIKFSNDAYDSRCPNHSLVWKIDPFSKEETFVTTNGVLGNPGSNTIHNWFQIEKYEDAYKLVYCPNVCPTCNHVCKDIGIYVYKTREMRLALTNVPFKVKFQKV