Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070553.t1 | XP_013454279.1 | 95.2 | 880 | 42 | 0 | 1 | 880 | 1 | 880 | 0.00E+00 | 1463.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070553.t1 | Q9C895 | 52.4 | 903 | 404 | 5 | 1 | 880 | 1 | 900 | 4.0e-215 | 749.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070553.t1 | A0A072UR29 | 95.2 | 880 | 42 | 0 | 1 | 880 | 1 | 880 | 0.0e+00 | 1463.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049159 | MS.gene070553 | 0.82935 | 5.41E-55 | -1.69E-46 |
| MS.gene049230 | MS.gene070553 | 0.805167 | 1.52E-49 | -1.69E-46 |
| MS.gene049378 | MS.gene070553 | 0.83801 | 3.71E-57 | -1.69E-46 |
| MS.gene049435 | MS.gene070553 | 0.805806 | 1.12E-49 | -1.69E-46 |
| MS.gene050226 | MS.gene070553 | 0.84881 | 4.84E-60 | -1.69E-46 |
| MS.gene050227 | MS.gene070553 | 0.856016 | 4.28E-62 | -1.69E-46 |
| MS.gene050228 | MS.gene070553 | 0.860376 | 2.16E-63 | -1.69E-46 |
| MS.gene050438 | MS.gene070553 | 0.801918 | 7.18E-49 | -1.69E-46 |
| MS.gene050442 | MS.gene070553 | 0.840232 | 9.85E-58 | -1.69E-46 |
| MS.gene050605 | MS.gene070553 | 0.825118 | 5.58E-54 | -1.69E-46 |
| MS.gene050623 | MS.gene070553 | -0.873405 | 1.51E-67 | -1.69E-46 |
| MS.gene050642 | MS.gene070553 | 0.828446 | 8.95E-55 | -1.69E-46 |
| MS.gene050719 | MS.gene070553 | 0.807457 | 5.01E-50 | -1.69E-46 |
| MS.gene050721 | MS.gene070553 | 0.805918 | 1.06E-49 | -1.69E-46 |
| MS.gene050724 | MS.gene070553 | 0.807311 | 5.38E-50 | -1.69E-46 |
| MS.gene050762 | MS.gene070553 | 0.826982 | 2.01E-54 | -1.69E-46 |
| MS.gene050763 | MS.gene070553 | 0.828524 | 8.57E-55 | -1.69E-46 |
| MS.gene050780 | MS.gene070553 | 0.821643 | 3.62E-53 | -1.69E-46 |
| MS.gene051210 | MS.gene070553 | 0.813551 | 2.42E-51 | -1.69E-46 |
| MS.gene051860 | MS.gene070553 | 0.833105 | 6.45E-56 | -1.69E-46 |
| MS.gene051983 | MS.gene070553 | 0.845943 | 2.96E-59 | -1.69E-46 |
| MS.gene052012 | MS.gene070553 | 0.821024 | 5.03E-53 | -1.69E-46 |
| MS.gene052077 | MS.gene070553 | 0.801139 | 1.04E-48 | -1.69E-46 |
| MS.gene053008 | MS.gene070553 | 0.806081 | 9.78E-50 | -1.69E-46 |
| MS.gene054379 | MS.gene070553 | 0.825606 | 4.28E-54 | -1.69E-46 |
| MS.gene054433 | MS.gene070553 | 0.804027 | 2.63E-49 | -1.69E-46 |
| MS.gene054467 | MS.gene070553 | 0.805006 | 1.64E-49 | -1.69E-46 |
| MS.gene054468 | MS.gene070553 | 0.824892 | 6.31E-54 | -1.69E-46 |
| MS.gene054559 | MS.gene070553 | 0.81175 | 5.99E-51 | -1.69E-46 |
| MS.gene054657 | MS.gene070553 | 0.802997 | 4.30E-49 | -1.69E-46 |
| MS.gene054658 | MS.gene070553 | 0.812204 | 4.77E-51 | -1.69E-46 |
| MS.gene05510 | MS.gene070553 | 0.815517 | 8.88E-52 | -1.69E-46 |
| MS.gene055356 | MS.gene070553 | 0.805011 | 1.64E-49 | -1.69E-46 |
| MS.gene055358 | MS.gene070553 | 0.822896 | 1.85E-53 | -1.69E-46 |
| MS.gene055701 | MS.gene070553 | 0.846167 | 2.57E-59 | -1.69E-46 |
| MS.gene05604 | MS.gene070553 | 0.801281 | 9.70E-49 | -1.69E-46 |
| MS.gene056506 | MS.gene070553 | 0.819119 | 1.37E-52 | -1.69E-46 |
| MS.gene056552 | MS.gene070553 | 0.824649 | 7.20E-54 | -1.69E-46 |
| MS.gene056979 | MS.gene070553 | 0.841886 | 3.62E-58 | -1.69E-46 |
| MS.gene057026 | MS.gene070553 | 0.81873 | 1.68E-52 | -1.69E-46 |
| MS.gene057200 | MS.gene070553 | 0.804743 | 1.87E-49 | -1.69E-46 |
| MS.gene058146 | MS.gene070553 | 0.833724 | 4.52E-56 | -1.69E-46 |
| MS.gene058435 | MS.gene070553 | 0.804315 | 2.29E-49 | -1.69E-46 |
| MS.gene058636 | MS.gene070553 | 0.814194 | 1.74E-51 | -1.69E-46 |
| MS.gene059128 | MS.gene070553 | 0.800328 | 1.52E-48 | -1.69E-46 |
| MS.gene059161 | MS.gene070553 | 0.817551 | 3.11E-52 | -1.69E-46 |
| MS.gene059609 | MS.gene070553 | 0.838724 | 2.43E-57 | -1.69E-46 |
| MS.gene059725 | MS.gene070553 | 0.814601 | 1.42E-51 | -1.69E-46 |
| MS.gene060322 | MS.gene070553 | 0.814682 | 1.36E-51 | -1.69E-46 |
| MS.gene060572 | MS.gene070553 | 0.806825 | 6.82E-50 | -1.69E-46 |
| MS.gene060637 | MS.gene070553 | 0.804745 | 1.86E-49 | -1.69E-46 |
| MS.gene060683 | MS.gene070553 | 0.836203 | 1.07E-56 | -1.69E-46 |
| MS.gene060685 | MS.gene070553 | 0.835941 | 1.25E-56 | -1.69E-46 |
| MS.gene060802 | MS.gene070553 | 0.839344 | 1.68E-57 | -1.69E-46 |
| MS.gene060803 | MS.gene070553 | 0.853723 | 1.98E-61 | -1.69E-46 |
| MS.gene060942 | MS.gene070553 | 0.805194 | 1.50E-49 | -1.69E-46 |
| MS.gene060998 | MS.gene070553 | 0.835591 | 1.54E-56 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070553.t1 | MTR_5g085010 | 96.364 | 880 | 32 | 0 | 1 | 880 | 1 | 880 | 0.0 | 1722 |
| MS.gene070553.t1 | MTR_1g068830 | 89.432 | 880 | 93 | 0 | 1 | 880 | 1 | 880 | 0.0 | 1495 |
| MS.gene070553.t1 | MTR_7g046250 | 31.058 | 879 | 598 | 6 | 4 | 880 | 5 | 877 | 5.98e-123 | 393 |
| MS.gene070553.t1 | MTR_7g046250 | 31.704 | 716 | 482 | 5 | 4 | 718 | 5 | 714 | 2.00e-94 | 313 |
| MS.gene070553.t1 | MTR_3g060950 | 73.203 | 153 | 24 | 3 | 711 | 861 | 61 | 198 | 2.21e-60 | 204 |
| MS.gene070553.t1 | MTR_5g065520 | 40.299 | 335 | 98 | 9 | 275 | 593 | 1 | 249 | 6.70e-46 | 171 |
| MS.gene070553.t1 | MTR_8g064230 | 82.143 | 84 | 15 | 0 | 252 | 335 | 2 | 85 | 2.81e-38 | 137 |
| MS.gene070553.t1 | MTR_5g065540 | 85.106 | 47 | 7 | 0 | 47 | 93 | 12 | 58 | 1.74e-19 | 84.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070553.t1 | AT1G55250 | 54.308 | 882 | 400 | 3 | 1 | 880 | 1 | 881 | 0.0 | 915 |
| MS.gene070553.t1 | AT1G55250 | 53.104 | 902 | 398 | 6 | 1 | 880 | 1 | 899 | 0.0 | 897 |
| MS.gene070553.t1 | AT1G55250 | 52.824 | 903 | 400 | 5 | 1 | 880 | 1 | 900 | 0.0 | 894 |
| MS.gene070553.t1 | AT1G55250 | 57.895 | 380 | 159 | 1 | 502 | 880 | 4 | 383 | 2.22e-141 | 424 |
| MS.gene070553.t1 | AT1G55250 | 55.838 | 394 | 159 | 2 | 502 | 880 | 4 | 397 | 3.24e-137 | 414 |
| MS.gene070553.t1 | AT2G44950 | 30.385 | 882 | 596 | 11 | 8 | 880 | 6 | 878 | 1.52e-116 | 376 |
| MS.gene070553.t1 | AT2G44950 | 29.552 | 670 | 455 | 10 | 8 | 669 | 6 | 666 | 3.64e-72 | 251 |
Find 159 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCCCTCCACAGAATCTTTC+TGG | 0.139617 | 5.4:+67130569 | None:intergenic |
| GATCTTGTATAGCCTGTTTC+TGG | 0.173211 | 5.4:+67131091 | None:intergenic |
| GAAGGAATTGAATTCTAAAT+TGG | 0.246983 | 5.4:-67125151 | MS.gene070553:CDS |
| CAGAATTCAGCTCAGCAATT+TGG | 0.247609 | 5.4:+67120386 | None:intergenic |
| GCTGAACAAGCTGCTGATTC+TGG | 0.251145 | 5.4:+67131124 | None:intergenic |
| TCAACATTGGATTGCTGAAT+TGG | 0.266671 | 5.4:-67125363 | MS.gene070553:CDS |
| TTTGTAGCTGGTTGATGATA+TGG | 0.269147 | 5.4:-67130824 | MS.gene070553:intron |
| CTTTGCGTCGGTTATCTACT+AGG | 0.292056 | 5.4:-67130504 | MS.gene070553:CDS |
| ACCTTTCATTTGCATCAAAC+TGG | 0.292093 | 5.4:+67122782 | None:intergenic |
| GTTACAGTTTGAAAAGTTAC+AGG | 0.298097 | 5.4:-67123843 | MS.gene070553:intron |
| GATTGATGATATGACGAAAA+AGG | 0.308345 | 5.4:-67130232 | MS.gene070553:CDS |
| TCAGGAGTTAGAATTTGTTT+TGG | 0.321414 | 5.4:-67123813 | MS.gene070553:CDS |
| AAAAGATTCTTTGAAAACTT+TGG | 0.324595 | 5.4:-67130773 | MS.gene070553:CDS |
| AGCACTCGTCTATATAATTC+TGG | 0.335894 | 5.4:+67130142 | None:intergenic |
| TCTGACATAAAACAACTTAC+AGG | 0.344586 | 5.4:-67130108 | MS.gene070553:intron |
| TTCCCACCTGGCAAACTCTA+AGG | 0.353582 | 5.4:+67121181 | None:intergenic |
| TTTCTGAGCTTCAAGATTCC+CGG | 0.355151 | 5.4:-67125605 | MS.gene070553:CDS |
| CTTTCAGATTCTCTAACTTC+TGG | 0.356059 | 5.4:+67122949 | None:intergenic |
| CTCTAGACCATGTTCATCTA+AGG | 0.361421 | 5.4:+67122891 | None:intergenic |
| AAAGTTACAGGAGGAGAATC+AGG | 0.371139 | 5.4:-67123831 | MS.gene070553:CDS |
| AACCTCGATTTCAGATATAT+AGG | 0.375681 | 5.4:+67122372 | None:intergenic |
| CATCCCCATTTCTTTAGATA+AGG | 0.377185 | 5.4:+67124872 | None:intergenic |
| AGATGAGGCTTCTTCTTGTC+TGG | 0.380963 | 5.4:+67132196 | None:intergenic |
| GCTCTCTCTGCAGGCTTCTC+AGG | 0.381182 | 5.4:+67125771 | None:intergenic |
| CAAAGGATCAACTTCAACAT+TGG | 0.382751 | 5.4:-67125376 | MS.gene070553:CDS |
| TGCAAACAAATGTGCTGAAC+AGG | 0.382951 | 5.4:-67124137 | MS.gene070553:CDS |
| AAAGATATCAAAGCTGAGTT+TGG | 0.385405 | 5.4:-67124909 | MS.gene070553:CDS |
| TTCAGCCTTATCTAAAGAAA+TGG | 0.386599 | 5.4:-67124877 | MS.gene070553:CDS |
| TTCCTTTCCTTCGTGTCCTC+CGG | 0.387864 | 5.4:-67130617 | MS.gene070553:CDS |
| CGAGATTACTAATTATGTTA+AGG | 0.396727 | 5.4:-67130536 | MS.gene070553:CDS |
| CAGGACTAACATTGCAAAAC+GGG | 0.396999 | 5.4:-67125175 | MS.gene070553:CDS |
| AGAGTTTGCCAGGTGGGAAT+TGG | 0.397987 | 5.4:-67121177 | MS.gene070553:CDS |
| GCTGCAGAAGCTGAAATAGA+AGG | 0.400466 | 5.4:-67122814 | MS.gene070553:CDS |
| AAAGCAATTTGAGGAACTTC+AGG | 0.401444 | 5.4:-67125562 | MS.gene070553:intron |
| CACTTCCCGAGTATACTCTT+TGG | 0.403272 | 5.4:-67125399 | MS.gene070553:CDS |
| CCTGCCTGTGGAACAGCATT+TGG | 0.404398 | 5.4:-67119870 | MS.gene070553:CDS |
| AAGCTGAAATAGAAGGGTTA+AGG | 0.408442 | 5.4:-67122807 | MS.gene070553:CDS |
| GTTGATGATATGGCTCTTCT+CGG | 0.410027 | 5.4:-67130814 | MS.gene070553:CDS |
| TCTTCTACTCTCTTCAAGTT+CGG | 0.410715 | 5.4:+67125878 | None:intergenic |
| TCAGCCTTATCTAAAGAAAT+GGG | 0.420197 | 5.4:-67124876 | MS.gene070553:CDS |
| GAAGCTTGAGGAAGAACTAA+TGG | 0.422228 | 5.4:-67120419 | MS.gene070553:CDS |
| ACACAGACCAGTATCTTGTT+TGG | 0.429741 | 5.4:+67125008 | None:intergenic |
| TGCTTCCTCCAGGCTGATGC+AGG | 0.438735 | 5.4:-67131159 | MS.gene070553:intron |
| CAGCCGCCTTAGAGTTTGCC+AGG | 0.439108 | 5.4:-67121187 | MS.gene070553:CDS |
| AGATACTGTTGATGATCATA+TGG | 0.440228 | 5.4:-67130461 | MS.gene070553:CDS |
| GCAGACAGAAGTCCAGAAAC+AGG | 0.440620 | 5.4:-67131103 | MS.gene070553:CDS |
| AGCTGAAATAGAAGGGTTAA+GGG | 0.445074 | 5.4:-67122806 | MS.gene070553:CDS |
| GCTCTTCTCGGGATACAAGC+TGG | 0.449889 | 5.4:-67130802 | MS.gene070553:CDS |
| CTCTTTAGCTTTAATAGCCT+CGG | 0.449984 | 5.4:+67122408 | None:intergenic |
| CAAATGTGCTGAACAGGATT+CGG | 0.450852 | 5.4:-67124131 | MS.gene070553:CDS |
| TCAAGATTTCTTTGTATGCA+TGG | 0.451174 | 5.4:+67119912 | None:intergenic |
| AATATTGACAAAGCAATTTG+AGG | 0.451416 | 5.4:-67125571 | MS.gene070553:CDS |
| ATCTAAAGAAATGGGGATGA+TGG | 0.454064 | 5.4:-67124868 | MS.gene070553:CDS |
| AATTACAAATAGTGAAGAAC+AGG | 0.462184 | 5.4:-67121365 | MS.gene070553:intron |
| GTTCAGCACATTTGTTTGCA+AGG | 0.462913 | 5.4:+67124140 | None:intergenic |
| CGTGAGACGCGGGAAGAAAC+GGG | 0.463036 | 5.4:+67132166 | None:intergenic |
| AATGCAAAAGGATGCAGCTA+TGG | 0.467843 | 5.4:-67125838 | MS.gene070553:CDS |
| TCCGTTCACTGCATCAGCAT+TGG | 0.477019 | 5.4:+67125803 | None:intergenic |
| CCTTTCATTTGCATCAAACT+GGG | 0.477201 | 5.4:+67122783 | None:intergenic |
| TCGTGAGACGCGGGAAGAAA+CGG | 0.486950 | 5.4:+67132165 | None:intergenic |
| CTACATAGAAGTAGATCATC+CGG | 0.493035 | 5.4:+67130598 | None:intergenic |
| TTATATCATGCAGGAGTTCA+CGG | 0.493537 | 5.4:-67120444 | MS.gene070553:intron |
| CGCCTTAGAGTTTGCCAGGT+GGG | 0.494648 | 5.4:-67121183 | MS.gene070553:CDS |
| TTCCACAGGCAGGACATTTG+CGG | 0.497983 | 5.4:+67119880 | None:intergenic |
| GAAGCCTGCAGAGAGAGCTA+TGG | 0.498135 | 5.4:-67125766 | MS.gene070553:CDS |
| ATGGAAAGAATTGAGGATAT+AGG | 0.501506 | 5.4:-67130442 | MS.gene070553:CDS |
| AGGCAGGACATTTGCGGTGA+CGG | 0.501630 | 5.4:+67119886 | None:intergenic |
| CGAGGCTATTAAAGCTAAAG+AGG | 0.502369 | 5.4:-67122407 | MS.gene070553:CDS |
| CTGTCCAAATGCTGTTCCAC+AGG | 0.503225 | 5.4:+67119866 | None:intergenic |
| AAGTACCTTCAGGATTATCA+AGG | 0.503978 | 5.4:+67130746 | None:intergenic |
| CTCTTCTCGGGATACAAGCT+GGG | 0.505460 | 5.4:-67130801 | MS.gene070553:CDS |
| CCTTTGCCAAAGAGTATACT+CGG | 0.508295 | 5.4:+67125393 | None:intergenic |
| GCAGGACTAACATTGCAAAA+CGG | 0.510569 | 5.4:-67125176 | MS.gene070553:CDS |
| AATCACTTCACGAAAATTGT+CGG | 0.513242 | 5.4:+67130206 | None:intergenic |
| CCCAGTTTGATGCAAATGAA+AGG | 0.514393 | 5.4:-67122783 | MS.gene070553:intron |
| CCAAATGCTGTTCCACAGGC+AGG | 0.515641 | 5.4:+67119870 | None:intergenic |
| GTTCACTGCATCAGCATTGG+AGG | 0.516207 | 5.4:+67125806 | None:intergenic |
| GCCTGGAGGATTATCAGAAA+AGG | 0.516683 | 5.4:-67123781 | MS.gene070553:intron |
| GAAGAGGCCAAACAAGATAC+TGG | 0.517357 | 5.4:-67125015 | MS.gene070553:intron |
| AAAAGGAATTGGATAGTGAA+AGG | 0.522583 | 5.4:-67121061 | MS.gene070553:intron |
| TTCTGAGCTTCAAGATTCCC+GGG | 0.523846 | 5.4:-67125604 | MS.gene070553:CDS |
| ATCATTGACAGAGTCTCTGC+AGG | 0.528509 | 5.4:-67125330 | MS.gene070553:intron |
| AGTCAGTCTGAAAATGCAAA+AGG | 0.529585 | 5.4:-67125850 | MS.gene070553:CDS |
| AAGAATTGAGGATATAGGTC+AGG | 0.532464 | 5.4:-67130437 | MS.gene070553:CDS |
| TGCAAAAGTGTATTAATGAA+AGG | 0.535858 | 5.4:-67125059 | MS.gene070553:CDS |
| TTTGCGTCGGTTATCTACTA+GGG | 0.536181 | 5.4:-67130503 | MS.gene070553:CDS |
| CATATGTATTATACCTTCCG+TGG | 0.537093 | 5.4:+67130396 | None:intergenic |
| AAGCCTGCAGAGAGAGCTAT+GGG | 0.539173 | 5.4:-67125765 | MS.gene070553:CDS |
| AATCAACCAACACTGGGATC+AGG | 0.540393 | 5.4:-67131007 | MS.gene070553:intron |
| TAATTGCAATCAACCAACAC+TGG | 0.541368 | 5.4:-67131014 | MS.gene070553:CDS |
| AAACCCATAGCTCTCTCTGC+AGG | 0.543797 | 5.4:+67125762 | None:intergenic |
| ATGCAAAAGGATGCAGCTAT+GGG | 0.546927 | 5.4:-67125837 | MS.gene070553:CDS |
| TATTAAAGCTAAAGAGGCTG+AGG | 0.549699 | 5.4:-67122401 | MS.gene070553:CDS |
| TCCTAGAGTCAGAAATGTCA+TGG | 0.550226 | 5.4:+67125103 | None:intergenic |
| GGAATTGGCAGATGCTGAGA+AGG | 0.550271 | 5.4:-67121162 | MS.gene070553:CDS |
| TAGATAACCGACGCAAAGCA+AGG | 0.550352 | 5.4:+67130509 | None:intergenic |
| ATCCAGAAAGATTCTGTGGA+GGG | 0.551161 | 5.4:-67130571 | MS.gene070553:CDS |
| GCTTGAATTTCGTGAGACGC+GGG | 0.552167 | 5.4:+67132156 | None:intergenic |
| CTCGGGATACAAGCTGGGAG+AGG | 0.556620 | 5.4:-67130796 | MS.gene070553:CDS |
| TTGATGATATGGCTCTTCTC+GGG | 0.557494 | 5.4:-67130813 | MS.gene070553:CDS |
| CAAAGCTGAGTTTGGTGTGA+TGG | 0.562082 | 5.4:-67124901 | MS.gene070553:CDS |
| TGATCATATGGAAAGAATTG+AGG | 0.563216 | 5.4:-67130449 | MS.gene070553:CDS |
| TCCAATGCTGATGCAGTGAA+CGG | 0.564453 | 5.4:-67125804 | MS.gene070553:CDS |
| GATTACCTTGATAATCCTGA+AGG | 0.565357 | 5.4:-67130751 | MS.gene070553:intron |
| TAACATTGCAAAACGGGAGA+AGG | 0.566402 | 5.4:-67125169 | MS.gene070553:CDS |
| AGCATCTGCCAATTCCCACC+TGG | 0.566723 | 5.4:+67121169 | None:intergenic |
| TCCATGACATTTCTGACTCT+AGG | 0.566902 | 5.4:-67125104 | MS.gene070553:CDS |
| CACGAAGATTTATCCACGGA+AGG | 0.568556 | 5.4:-67130409 | MS.gene070553:intron |
| CTGAAGCTGCTTGTGAACAA+AGG | 0.572664 | 5.4:-67122843 | MS.gene070553:CDS |
| GAGGGAGGACTGTAATATCA+AGG | 0.573477 | 5.4:-67122188 | MS.gene070553:intron |
| AGGAAGCCCTTGCTTTGCGT+CGG | 0.576073 | 5.4:-67130516 | MS.gene070553:CDS |
| TTCAATTGAATTCTTCAACT+CGG | 0.577121 | 5.4:+67125737 | None:intergenic |
| CCAACGTCTATTACAGCAAG+TGG | 0.577654 | 5.4:-67122215 | MS.gene070553:CDS |
| CCGAGTATACTCTTTGGCAA+AGG | 0.579298 | 5.4:-67125393 | MS.gene070553:CDS |
| TCTTGAAATCAAGATGGAAG+AGG | 0.579606 | 5.4:-67125031 | MS.gene070553:CDS |
| ATCCGGAGGACACGAAGGAA+AGG | 0.581923 | 5.4:+67130615 | None:intergenic |
| ATTACAGCAAGTGGCTGAGA+GGG | 0.584569 | 5.4:-67122206 | MS.gene070553:CDS |
| AATAAAGTCGTCAAAAGCAA+TGG | 0.585648 | 5.4:-67124107 | MS.gene070553:intron |
| GAATGATCTTGAAATCAAGA+TGG | 0.585926 | 5.4:-67125037 | MS.gene070553:CDS |
| CTGCAGAAGCTGAAATAGAA+GGG | 0.587571 | 5.4:-67122813 | MS.gene070553:CDS |
| TGCTTGAATTTCGTGAGACG+CGG | 0.591340 | 5.4:+67132155 | None:intergenic |
| GAAACGGGAGTGAGAAGATG+AGG | 0.598522 | 5.4:+67132181 | None:intergenic |
| CGCTAAGAGAGAAATTGAGT+GGG | 0.598649 | 5.4:-67124779 | MS.gene070553:CDS |
| AACCTATATATCTGAAATCG+AGG | 0.599571 | 5.4:-67122374 | MS.gene070553:intron |
| GAATTCAATTGAAGAAGCGA+AGG | 0.600369 | 5.4:-67125727 | MS.gene070553:intron |
| TGGAAACACAGTTGAAGCGA+TGG | 0.602141 | 5.4:-67124848 | MS.gene070553:CDS |
| ATTAATCCAGAAAGATTCTG+TGG | 0.603677 | 5.4:-67130575 | MS.gene070553:CDS |
| CAATAACCTGATCCCAGTGT+TGG | 0.604541 | 5.4:+67131001 | None:intergenic |
| TGGAGTCTGCGGTTAATGCA+AGG | 0.604681 | 5.4:-67125131 | MS.gene070553:CDS |
| AGATAACCGACGCAAAGCAA+GGG | 0.606298 | 5.4:+67130510 | None:intergenic |
| CGAGCAGATTCAGAAAGATG+TGG | 0.606867 | 5.4:-67121093 | MS.gene070553:CDS |
| TCGCTAAGAGAGAAATTGAG+TGG | 0.608930 | 5.4:-67124780 | MS.gene070553:CDS |
| CACCGCAAATGTCCTGCCTG+TGG | 0.609399 | 5.4:-67119882 | MS.gene070553:CDS |
| AAACACAAGGAGTATACTGT+TGG | 0.609461 | 5.4:-67130168 | MS.gene070553:CDS |
| CTGTTCTGACCGTCCAAAGG+AGG | 0.610548 | 5.4:-67120290 | MS.gene070553:intron |
| GAATTCTAAATTGGAGTCTG+CGG | 0.618680 | 5.4:-67125142 | MS.gene070553:CDS |
| ACAGCAAGTGGCTGAGAGGG+AGG | 0.618824 | 5.4:-67122203 | MS.gene070553:CDS |
| CTTTGGACGGTCAGAACAGA+CGG | 0.621473 | 5.4:+67120294 | None:intergenic |
| AAGAGAGAAATTGAGTGGGA+AGG | 0.622883 | 5.4:-67124775 | MS.gene070553:intron |
| AGGAGAGTTTGACGAAATCG+TGG | 0.625770 | 5.4:-67125901 | MS.gene070553:CDS |
| CGTCTGTTCTGACCGTCCAA+AGG | 0.628492 | 5.4:-67120293 | MS.gene070553:CDS |
| AATTGCAATCAACCAACACT+GGG | 0.631357 | 5.4:-67131013 | MS.gene070553:CDS |
| GAGTTCACGGAAGAAGCTTG+AGG | 0.633563 | 5.4:-67120431 | MS.gene070553:CDS |
| ACAGTTTGAAAAGTTACAGG+AGG | 0.634527 | 5.4:-67123840 | MS.gene070553:intron |
| CAGAAAGATTCTGTGGAGGG+AGG | 0.638260 | 5.4:-67130568 | MS.gene070553:CDS |
| TTTGGACATGTATGGCCTGG+AGG | 0.642076 | 5.4:-67123795 | MS.gene070553:CDS |
| CAGCCTTATCTAAAGAAATG+GGG | 0.644025 | 5.4:-67124875 | MS.gene070553:CDS |
| AATCCAGAAAGATTCTGTGG+AGG | 0.648780 | 5.4:-67130572 | MS.gene070553:CDS |
| TATTACAGCAAGTGGCTGAG+AGG | 0.658845 | 5.4:-67122207 | MS.gene070553:CDS |
| CCACTTGCTGTAATAGACGT+TGG | 0.660621 | 5.4:+67122215 | None:intergenic |
| AGATCATCCGGAGGACACGA+AGG | 0.660758 | 5.4:+67130610 | None:intergenic |
| TACTCTCCATGCAAAACACA+AGG | 0.662712 | 5.4:-67130181 | MS.gene070553:CDS |
| GCTTGTATCAGAAAGCGTGA+AGG | 0.670954 | 5.4:-67121482 | MS.gene070553:CDS |
| CTTACACGAAGATTTATCCA+CGG | 0.672505 | 5.4:-67130413 | MS.gene070553:CDS |
| CTTTGCCAAAGAGTATACTC+GGG | 0.675113 | 5.4:+67125394 | None:intergenic |
| CAAACTCTAAGGCGGCTGCG+AGG | 0.683577 | 5.4:+67121192 | None:intergenic |
| CCGCCTTAGAGTTTGCCAGG+TGG | 0.688256 | 5.4:-67121184 | MS.gene070553:CDS |
| CCACCTGGCAAACTCTAAGG+CGG | 0.716860 | 5.4:+67121184 | None:intergenic |
| CATAGAAGTAGATCATCCGG+AGG | 0.734057 | 5.4:+67130601 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 67119839 | 67132242 | 67119839 | ID=MS.gene070553 |
| chr5.4 | mRNA | 67119839 | 67132242 | 67119839 | ID=MS.gene070553.t1;Parent=MS.gene070553 |
| chr5.4 | exon | 67132132 | 67132242 | 67132132 | ID=MS.gene070553.t1.exon1;Parent=MS.gene070553.t1 |
| chr5.4 | CDS | 67132132 | 67132242 | 67132132 | ID=cds.MS.gene070553.t1;Parent=MS.gene070553.t1 |
| chr5.4 | exon | 67131008 | 67131169 | 67131008 | ID=MS.gene070553.t1.exon2;Parent=MS.gene070553.t1 |
| chr5.4 | CDS | 67131008 | 67131169 | 67131008 | ID=cds.MS.gene070553.t1;Parent=MS.gene070553.t1 |
| chr5.4 | exon | 67130752 | 67130839 | 67130752 | ID=MS.gene070553.t1.exon3;Parent=MS.gene070553.t1 |
| chr5.4 | CDS | 67130752 | 67130839 | 67130752 | ID=cds.MS.gene070553.t1;Parent=MS.gene070553.t1 |
| chr5.4 | exon | 67130410 | 67130640 | 67130410 | ID=MS.gene070553.t1.exon4;Parent=MS.gene070553.t1 |
| chr5.4 | CDS | 67130410 | 67130640 | 67130410 | ID=cds.MS.gene070553.t1;Parent=MS.gene070553.t1 |
| chr5.4 | exon | 67130109 | 67130276 | 67130109 | ID=MS.gene070553.t1.exon5;Parent=MS.gene070553.t1 |
| chr5.4 | CDS | 67130109 | 67130276 | 67130109 | ID=cds.MS.gene070553.t1;Parent=MS.gene070553.t1 |
| chr5.4 | exon | 67125728 | 67125921 | 67125728 | ID=MS.gene070553.t1.exon6;Parent=MS.gene070553.t1 |
| chr5.4 | CDS | 67125728 | 67125921 | 67125728 | ID=cds.MS.gene070553.t1;Parent=MS.gene070553.t1 |
| chr5.4 | exon | 67125563 | 67125646 | 67125563 | ID=MS.gene070553.t1.exon7;Parent=MS.gene070553.t1 |
| chr5.4 | CDS | 67125563 | 67125646 | 67125563 | ID=cds.MS.gene070553.t1;Parent=MS.gene070553.t1 |
| chr5.4 | exon | 67125331 | 67125450 | 67125331 | ID=MS.gene070553.t1.exon8;Parent=MS.gene070553.t1 |
| chr5.4 | CDS | 67125331 | 67125450 | 67125331 | ID=cds.MS.gene070553.t1;Parent=MS.gene070553.t1 |
| chr5.4 | exon | 67125016 | 67125202 | 67125016 | ID=MS.gene070553.t1.exon9;Parent=MS.gene070553.t1 |
| chr5.4 | CDS | 67125016 | 67125202 | 67125016 | ID=cds.MS.gene070553.t1;Parent=MS.gene070553.t1 |
| chr5.4 | exon | 67124776 | 67124936 | 67124776 | ID=MS.gene070553.t1.exon10;Parent=MS.gene070553.t1 |
| chr5.4 | CDS | 67124776 | 67124936 | 67124776 | ID=cds.MS.gene070553.t1;Parent=MS.gene070553.t1 |
| chr5.4 | exon | 67124108 | 67124179 | 67124108 | ID=MS.gene070553.t1.exon11;Parent=MS.gene070553.t1 |
| chr5.4 | CDS | 67124108 | 67124179 | 67124108 | ID=cds.MS.gene070553.t1;Parent=MS.gene070553.t1 |
| chr5.4 | exon | 67123782 | 67123858 | 67123782 | ID=MS.gene070553.t1.exon12;Parent=MS.gene070553.t1 |
| chr5.4 | CDS | 67123782 | 67123858 | 67123782 | ID=cds.MS.gene070553.t1;Parent=MS.gene070553.t1 |
| chr5.4 | exon | 67122784 | 67122975 | 67122784 | ID=MS.gene070553.t1.exon13;Parent=MS.gene070553.t1 |
| chr5.4 | CDS | 67122784 | 67122975 | 67122784 | ID=cds.MS.gene070553.t1;Parent=MS.gene070553.t1 |
| chr5.4 | exon | 67122375 | 67122447 | 67122375 | ID=MS.gene070553.t1.exon14;Parent=MS.gene070553.t1 |
| chr5.4 | CDS | 67122375 | 67122447 | 67122375 | ID=cds.MS.gene070553.t1;Parent=MS.gene070553.t1 |
| chr5.4 | exon | 67122189 | 67122275 | 67122189 | ID=MS.gene070553.t1.exon15;Parent=MS.gene070553.t1 |
| chr5.4 | CDS | 67122189 | 67122275 | 67122189 | ID=cds.MS.gene070553.t1;Parent=MS.gene070553.t1 |
| chr5.4 | exon | 67121366 | 67121503 | 67121366 | ID=MS.gene070553.t1.exon16;Parent=MS.gene070553.t1 |
| chr5.4 | CDS | 67121366 | 67121503 | 67121366 | ID=cds.MS.gene070553.t1;Parent=MS.gene070553.t1 |
| chr5.4 | exon | 67121062 | 67121264 | 67121062 | ID=MS.gene070553.t1.exon17;Parent=MS.gene070553.t1 |
| chr5.4 | CDS | 67121062 | 67121264 | 67121062 | ID=cds.MS.gene070553.t1;Parent=MS.gene070553.t1 |
| chr5.4 | exon | 67120291 | 67120453 | 67120291 | ID=MS.gene070553.t1.exon18;Parent=MS.gene070553.t1 |
| chr5.4 | CDS | 67120291 | 67120453 | 67120291 | ID=cds.MS.gene070553.t1;Parent=MS.gene070553.t1 |
| chr5.4 | exon | 67119839 | 67119970 | 67119839 | ID=MS.gene070553.t1.exon19;Parent=MS.gene070553.t1 |
| chr5.4 | CDS | 67119839 | 67119970 | 67119839 | ID=cds.MS.gene070553.t1;Parent=MS.gene070553.t1 |
| Gene Sequence |
| Protein sequence |
