Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070982.t1 | XP_013469722.1 | 93.3 | 150 | 8 | 1 | 1 | 148 | 113 | 262 | 1.20E-70 | 275.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070982.t1 | A0A072W0C2 | 93.3 | 150 | 8 | 1 | 1 | 148 | 113 | 262 | 8.5e-71 | 275.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049171 | MS.gene070982 | -0.819869 | 9.26E-53 | -1.69E-46 |
| MS.gene049217 | MS.gene070982 | -0.823108 | 1.65E-53 | -1.69E-46 |
| MS.gene049281 | MS.gene070982 | 0.825321 | 5.00E-54 | -1.69E-46 |
| MS.gene049285 | MS.gene070982 | -0.835209 | 1.92E-56 | -1.69E-46 |
| MS.gene049313 | MS.gene070982 | 0.819298 | 1.25E-52 | -1.69E-46 |
| MS.gene049320 | MS.gene070982 | -0.848009 | 8.05E-60 | -1.69E-46 |
| MS.gene049483 | MS.gene070982 | 0.842796 | 2.08E-58 | -1.69E-46 |
| MS.gene049609 | MS.gene070982 | -0.808067 | 3.72E-50 | -1.69E-46 |
| MS.gene049627 | MS.gene070982 | -0.802386 | 5.75E-49 | -1.69E-46 |
| MS.gene05010 | MS.gene070982 | -0.811718 | 6.08E-51 | -1.69E-46 |
| MS.gene050129 | MS.gene070982 | 0.829409 | 5.23E-55 | -1.69E-46 |
| MS.gene050163 | MS.gene070982 | -0.821519 | 3.87E-53 | -1.69E-46 |
| MS.gene050165 | MS.gene070982 | -0.815543 | 8.76E-52 | -1.69E-46 |
| MS.gene050363 | MS.gene070982 | 0.809925 | 1.49E-50 | -1.69E-46 |
| MS.gene050381 | MS.gene070982 | 0.809049 | 2.29E-50 | -1.69E-46 |
| MS.gene050504 | MS.gene070982 | -0.839749 | 1.32E-57 | -1.69E-46 |
| MS.gene050518 | MS.gene070982 | -0.802104 | 6.57E-49 | -1.69E-46 |
| MS.gene050612 | MS.gene070982 | -0.820778 | 5.73E-53 | -1.69E-46 |
| MS.gene050675 | MS.gene070982 | -0.806428 | 8.26E-50 | -1.69E-46 |
| MS.gene050699 | MS.gene070982 | -0.834754 | 2.50E-56 | -1.69E-46 |
| MS.gene050772 | MS.gene070982 | -0.812255 | 4.65E-51 | -1.69E-46 |
| MS.gene050986 | MS.gene070982 | -0.836181 | 1.09E-56 | -1.69E-46 |
| MS.gene051127 | MS.gene070982 | 0.806648 | 7.43E-50 | -1.69E-46 |
| MS.gene05141 | MS.gene070982 | 0.806714 | 7.19E-50 | -1.69E-46 |
| MS.gene051810 | MS.gene070982 | -0.810162 | 1.32E-50 | -1.69E-46 |
| MS.gene051946 | MS.gene070982 | 0.810604 | 1.06E-50 | -1.69E-46 |
| MS.gene052164 | MS.gene070982 | -0.825664 | 4.14E-54 | -1.69E-46 |
| MS.gene052486 | MS.gene070982 | -0.803565 | 3.28E-49 | -1.69E-46 |
| MS.gene053203 | MS.gene070982 | -0.802427 | 5.64E-49 | -1.69E-46 |
| MS.gene053213 | MS.gene070982 | -0.823932 | 1.06E-53 | -1.69E-46 |
| MS.gene053286 | MS.gene070982 | -0.820414 | 6.95E-53 | -1.69E-46 |
| MS.gene053420 | MS.gene070982 | 0.807925 | 3.98E-50 | -1.69E-46 |
| MS.gene053549 | MS.gene070982 | 0.825514 | 4.50E-54 | -1.69E-46 |
| MS.gene053561 | MS.gene070982 | 0.805425 | 1.34E-49 | -1.69E-46 |
| MS.gene054616 | MS.gene070982 | 0.833847 | 4.21E-56 | -1.69E-46 |
| MS.gene054642 | MS.gene070982 | -0.819791 | 9.65E-53 | -1.69E-46 |
| MS.gene05470 | MS.gene070982 | -0.812279 | 4.59E-51 | -1.69E-46 |
| MS.gene054954 | MS.gene070982 | -0.833391 | 5.48E-56 | -1.69E-46 |
| MS.gene054959 | MS.gene070982 | 0.802128 | 6.50E-49 | -1.69E-46 |
| MS.gene055154 | MS.gene070982 | 0.816075 | 6.66E-52 | -1.69E-46 |
| MS.gene055362 | MS.gene070982 | -0.85098 | 1.20E-60 | -1.69E-46 |
| MS.gene055502 | MS.gene070982 | -0.839263 | 1.76E-57 | -1.69E-46 |
| MS.gene055577 | MS.gene070982 | -0.810294 | 1.24E-50 | -1.69E-46 |
| MS.gene056083 | MS.gene070982 | -0.802531 | 5.37E-49 | -1.69E-46 |
| MS.gene056408 | MS.gene070982 | 0.807043 | 6.13E-50 | -1.69E-46 |
| MS.gene056504 | MS.gene070982 | -0.81188 | 5.61E-51 | -1.69E-46 |
| MS.gene056550 | MS.gene070982 | 0.831947 | 1.25E-55 | -1.69E-46 |
| MS.gene056657 | MS.gene070982 | -0.826175 | 3.13E-54 | -1.69E-46 |
| MS.gene056692 | MS.gene070982 | -0.80446 | 2.14E-49 | -1.69E-46 |
| MS.gene05837 | MS.gene070982 | -0.803225 | 3.86E-49 | -1.69E-46 |
| MS.gene058419 | MS.gene070982 | -0.833832 | 4.25E-56 | -1.69E-46 |
| MS.gene058544 | MS.gene070982 | -0.810794 | 9.65E-51 | -1.69E-46 |
| MS.gene058773 | MS.gene070982 | -0.829203 | 5.87E-55 | -1.69E-46 |
| MS.gene059022 | MS.gene070982 | -0.867341 | 1.47E-65 | -1.69E-46 |
| MS.gene059023 | MS.gene070982 | -0.827149 | 1.84E-54 | -1.69E-46 |
| MS.gene059677 | MS.gene070982 | -0.84679 | 1.74E-59 | -1.69E-46 |
| MS.gene059718 | MS.gene070982 | -0.844012 | 9.84E-59 | -1.69E-46 |
| MS.gene059865 | MS.gene070982 | -0.814091 | 1.84E-51 | -1.69E-46 |
| MS.gene060703 | MS.gene070982 | -0.824713 | 6.95E-54 | -1.69E-46 |
| MS.gene060762 | MS.gene070982 | -0.81205 | 5.15E-51 | -1.69E-46 |
| MS.gene06077 | MS.gene070982 | -0.849976 | 2.29E-60 | -1.69E-46 |
| MS.gene06092 | MS.gene070982 | -0.804641 | 1.96E-49 | -1.69E-46 |
| MS.gene061150 | MS.gene070982 | -0.808049 | 3.75E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070982.t1 | MTR_1g102510 | 94.667 | 150 | 6 | 1 | 1 | 148 | 113 | 262 | 5.25e-93 | 270 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070982.t1 | AT5G51680 | 41.096 | 146 | 78 | 4 | 7 | 148 | 202 | 343 | 6.91e-14 | 67.8 |
Find 33 sgRNAs with CRISPR-Local
Find 44 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGAAAAGTTAAGTTCAAGTT+TGG | 0.340393 | 1.4:-79286789 | MS.gene070982:CDS |
| ACTGGAAGGTCAAGTTTAAC+TGG | 0.341492 | 1.4:-79286887 | MS.gene070982:CDS |
| ACCACGAGAAAGCAAGCTTT+TGG | 0.370273 | 1.4:-79286320 | MS.gene070982:CDS |
| AAGCAACTCAAATGAACAAA+TGG | 0.378323 | 1.4:-79286652 | MS.gene070982:CDS |
| CCTTTGTTTCCGCCAAAGTC+TGG | 0.387487 | 1.4:-79286839 | MS.gene070982:CDS |
| TTCAAGTTTGGTGGAACTAA+AGG | 0.388380 | 1.4:-79286777 | MS.gene070982:CDS |
| AGTATAAGCAGCTATGAAAC+TGG | 0.406907 | 1.4:-79286905 | MS.gene070982:CDS |
| ATGGAACAAGTTACCTTTGG+TGG | 0.430078 | 1.4:+79286626 | None:intergenic |
| TCAAGTTTGGTGGAACTAAA+GGG | 0.439502 | 1.4:-79286776 | MS.gene070982:CDS |
| AAAGTTAAGTTCAAGTTTGG+TGG | 0.448826 | 1.4:-79286786 | MS.gene070982:CDS |
| CTCTCCAGCTTCGTCGGAGA+TGG | 0.451790 | 1.4:-79286933 | MS.gene070982:CDS |
| CTTTCAAGAAAACCAGACTT+TGG | 0.454747 | 1.4:+79286827 | None:intergenic |
| CTTTAGACTAGTTTCAGCCT+AGG | 0.480571 | 1.4:+79286236 | None:intergenic |
| CTATCCATCTCCGACGAAGC+TGG | 0.483008 | 1.4:+79286929 | None:intergenic |
| TTTAGACTAGTTTCAGCCTA+GGG | 0.498911 | 1.4:+79286237 | None:intergenic |
| AGCAACTCAAATGAACAAAT+GGG | 0.509084 | 1.4:-79286651 | MS.gene070982:CDS |
| TCAAGAAAACCAGACTTTGG+CGG | 0.522040 | 1.4:+79286830 | None:intergenic |
| ACAAGCAGCAGTATCATTGA+AGG | 0.522761 | 1.4:-79286281 | MS.gene070982:CDS |
| TCCGACGAAGCTGGAGAGGA+TGG | 0.522845 | 1.4:+79286938 | None:intergenic |
| CCAGACTTTGGCGGAAACAA+AGG | 0.526444 | 1.4:+79286839 | None:intergenic |
| ATAATTAACTCTCCAAGTGT+TGG | 0.527351 | 1.4:+79286704 | None:intergenic |
| GAGGATGGAGTTTGCTGTAG+TGG | 0.531543 | 1.4:+79286953 | None:intergenic |
| TCCATCCTCTCCAGCTTCGT+CGG | 0.537624 | 1.4:-79286939 | MS.gene070982:CDS |
| TCAAGGAAGCAACCAACACT+TGG | 0.542435 | 1.4:-79286716 | MS.gene070982:CDS |
| TTGCTTCCTTGACATATCAC+AGG | 0.543111 | 1.4:+79286727 | None:intergenic |
| TCCAAAAGCTTGCTTTCTCG+TGG | 0.546204 | 1.4:+79286319 | None:intergenic |
| ATTATACCTGTGATATGTCA+AGG | 0.573360 | 1.4:-79286733 | MS.gene070982:CDS |
| TGAAGAGAAAGTACTACCCT+AGG | 0.596271 | 1.4:-79286253 | MS.gene070982:CDS |
| CATCTCCGACGAAGCTGGAG+AGG | 0.596381 | 1.4:+79286934 | None:intergenic |
| TGGAGTTTGCTGTAGTGGCA+TGG | 0.598354 | 1.4:+79286958 | None:intergenic |
| GAACAAATGGGATCCACCAA+AGG | 0.609336 | 1.4:-79286639 | MS.gene070982:intron |
| TAAGCAGCTATGAAACTGGA+AGG | 0.618972 | 1.4:-79286901 | MS.gene070982:CDS |
| CAAGCAGCAGTATCATTGAA+GGG | 0.643098 | 1.4:-79286280 | MS.gene070982:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AACGTTTATTGACATAAAAA+TGG | + | chr1.4:79286590-79286609 | None:intergenic | 20.0% |
| !! | ATTCAAATTAGCATCTAAAT+TGG | - | chr1.4:79286749-79286768 | MS.gene070982:CDS | 20.0% |
| !!! | AATTGGTTACTTTTTAAGTA+TGG | - | chr1.4:79286766-79286785 | MS.gene070982:CDS | 20.0% |
| ! | ATAATCTCATCGAGAAAAAT+TGG | + | chr1.4:79286731-79286750 | None:intergenic | 25.0% |
| ! | CTAAAACGTTGAATTAAGAT+CGG | - | chr1.4:79286648-79286667 | MS.gene070982:CDS | 25.0% |
| ! | TATGTCAATAAACGTTTCTT+TGG | - | chr1.4:79286594-79286613 | MS.gene070982:intron | 25.0% |
| !! | TGAAAAGTTAAGTTCAAGTT+TGG | - | chr1.4:79286405-79286424 | MS.gene070982:intron | 25.0% |
| AAAATGGAACAAGTTACCTT+TGG | + | chr1.4:79286574-79286593 | None:intergenic | 30.0% | |
| AAGCAACTCAAATGAACAAA+TGG | - | chr1.4:79286542-79286561 | MS.gene070982:intron | 30.0% | |
| AGCAACTCAAATGAACAAAT+GGG | - | chr1.4:79286543-79286562 | MS.gene070982:intron | 30.0% | |
| ATAATTAACTCTCCAAGTGT+TGG | + | chr1.4:79286493-79286512 | None:intergenic | 30.0% | |
| ATTATACCTGTGATATGTCA+AGG | - | chr1.4:79286461-79286480 | MS.gene070982:intron | 30.0% | |
| TGAAGCAAAAGAGTAAATGT+TGG | + | chr1.4:79286843-79286862 | None:intergenic | 30.0% | |
| ! | AAAGTTAAGTTCAAGTTTGG+TGG | - | chr1.4:79286408-79286427 | MS.gene070982:intron | 30.0% |
| ! | GACTTTGTATTTGGAATACT+TGG | + | chr1.4:79286698-79286717 | None:intergenic | 30.0% |
| !!! | AAAGTCTGGTTTTCTTGAAA+GGG | - | chr1.4:79286369-79286388 | MS.gene070982:intron | 30.0% |
| !!! | TTTAACTGGTTCTGCTTTTT+TGG | - | chr1.4:79286321-79286340 | MS.gene070982:CDS | 30.0% |
| AGTATAAGCAGCTATGAAAC+TGG | - | chr1.4:79286289-79286308 | MS.gene070982:CDS | 35.0% | |
| CTTTCAAGAAAACCAGACTT+TGG | + | chr1.4:79286370-79286389 | None:intergenic | 35.0% | |
| TCGTAGTCTGACTTTGTATT+TGG | + | chr1.4:79286707-79286726 | None:intergenic | 35.0% | |
| ! | GGTTTTCTTGAAAGGGATAT+TGG | - | chr1.4:79286376-79286395 | MS.gene070982:intron | 35.0% |
| ! | TCAAGTTTGGTGGAACTAAA+GGG | - | chr1.4:79286418-79286437 | MS.gene070982:intron | 35.0% |
| ! | TTCAAGTTTGGTGGAACTAA+AGG | - | chr1.4:79286417-79286436 | MS.gene070982:intron | 35.0% |
| !!! | CAAAGTCTGGTTTTCTTGAA+AGG | - | chr1.4:79286368-79286387 | MS.gene070982:intron | 35.0% |
| ACAAGCAGCAGTATCATTGA+AGG | - | chr1.4:79286913-79286932 | MS.gene070982:CDS | 40.0% | |
| ACTGGAAGGTCAAGTTTAAC+TGG | - | chr1.4:79286307-79286326 | MS.gene070982:CDS | 40.0% | |
| ATGGAACAAGTTACCTTTGG+TGG | + | chr1.4:79286571-79286590 | None:intergenic | 40.0% | |
| CAAGCAGCAGTATCATTGAA+GGG | - | chr1.4:79286914-79286933 | MS.gene070982:CDS | 40.0% | |
| TAAGCAGCTATGAAACTGGA+AGG | - | chr1.4:79286293-79286312 | MS.gene070982:CDS | 40.0% | |
| TCAAGAAAACCAGACTTTGG+CGG | + | chr1.4:79286367-79286386 | None:intergenic | 40.0% | |
| TTGCTTCCTTGACATATCAC+AGG | + | chr1.4:79286470-79286489 | None:intergenic | 40.0% | |
| !! | TGAAGAGAAAGTACTACCCT+AGG | - | chr1.4:79286941-79286960 | MS.gene070982:CDS | 40.0% |
| GAACAAATGGGATCCACCAA+AGG | - | chr1.4:79286555-79286574 | MS.gene070982:intron | 45.0% | |
| TCAAGGAAGCAACCAACACT+TGG | - | chr1.4:79286478-79286497 | MS.gene070982:intron | 45.0% | |
| ! | ACCACGAGAAAGCAAGCTTT+TGG | - | chr1.4:79286874-79286893 | MS.gene070982:CDS | 45.0% |
| ! | TCCAAAAGCTTGCTTTCTCG+TGG | + | chr1.4:79286878-79286897 | None:intergenic | 45.0% |
| CCAGACTTTGGCGGAAACAA+AGG | + | chr1.4:79286358-79286377 | None:intergenic | 50.0% | |
| ! | CCTTTGTTTCCGCCAAAGTC+TGG | - | chr1.4:79286355-79286374 | MS.gene070982:intron | 50.0% |
| !! | GAGGATGGAGTTTGCTGTAG+TGG | + | chr1.4:79286244-79286263 | None:intergenic | 50.0% |
| CTATCCATCTCCGACGAAGC+TGG | + | chr1.4:79286268-79286287 | None:intergenic | 55.0% | |
| TCCATCCTCTCCAGCTTCGT+CGG | - | chr1.4:79286255-79286274 | MS.gene070982:CDS | 55.0% | |
| CATCTCCGACGAAGCTGGAG+AGG | + | chr1.4:79286263-79286282 | None:intergenic | 60.0% | |
| CTCTCCAGCTTCGTCGGAGA+TGG | - | chr1.4:79286261-79286280 | MS.gene070982:CDS | 60.0% | |
| TCCGACGAAGCTGGAGAGGA+TGG | + | chr1.4:79286259-79286278 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 79286238 | 79286978 | 79286238 | ID=MS.gene070982 |
| chr1.4 | mRNA | 79286238 | 79286978 | 79286238 | ID=MS.gene070982.t1;Parent=MS.gene070982 |
| chr1.4 | exon | 79286640 | 79286978 | 79286640 | ID=MS.gene070982.t1.exon1;Parent=MS.gene070982.t1 |
| chr1.4 | CDS | 79286640 | 79286978 | 79286640 | ID=cds.MS.gene070982.t1;Parent=MS.gene070982.t1 |
| chr1.4 | exon | 79286238 | 79286345 | 79286238 | ID=MS.gene070982.t1.exon2;Parent=MS.gene070982.t1 |
| chr1.4 | CDS | 79286238 | 79286345 | 79286238 | ID=cds.MS.gene070982.t1;Parent=MS.gene070982.t1 |
| Gene Sequence |
| Protein sequence |
